Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys peptideRafal Fudala
Center of Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107, USA
Curr Pharm Biotechnol 12:834-8. 2011
..The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions...
- Fluorescence detection of hyaluronidase
Rafal Fudala
Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
J Photochem Photobiol B 104:473-7. 2011
..This in turn, can be utilized for the construction of a robust but reliable HA-ase sensing device...
- Lifetime-based sensing of the hyaluronidase using fluorescein labeled hyaluronic acid
Rafal Fudala
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
J Photochem Photobiol B 106:69-73. 2012
..We demonstrate that the calibration of the sensing response is the same for the average lifetime as for a single exponential decay approximation, which significantly simplifies the analysis of the sensing measurements...
Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAngulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC)Ryan M Rich
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA
Anal Bioanal Chem 405:4887-94. 2013
..We then show that both time gating and FLCS may be used successfully with ADOTA-labeled HA to detect the presence of hyaluronidase, the overexpression of which has been observed in many types of cancer...
- Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy
Ryan M Rich
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
J Photochem Photobiol B 116:7-12. 2012
..The method we present provides a sensitive measure of hyaluronidase activity and requires extremely small amounts of the HA substrate...
- Long-lived bright red emitting azaoxa-triangulenium fluorophores
Badri P Maliwal
Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States of America
PLoS ONE 8:e63043. 2013
..The fluorescence lifetime facilitated gating of cellular nanosecond autofluorescence should be of considerable help in in vitro and in vivo applications...
- Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore
Ryan M Rich
Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA
Anal Bioanal Chem 405:2065-75. 2013
..This time-gating eliminates over 96 % of autofluorescence. Analysis using a variable time-gate may enable quantitative determination of the bound probe without the contributions from the background...
- Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain
Krishna Midde
Department of Cell Biology and Anatomy, University of North Texas, Health Science Center, Fort Worth, Texas 76107, USA
J Biol Chem 288:7012-23. 2013
..A simple theory was developed to account for this fact...
- Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays
Ryan Rich
Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
Anal Bioanal Chem 404:2223-31. 2012
..The analysis provides a possible mechanism by which the SIF/polymer-coated p-Chips allow a highly sensitive immunoassay and, as a result, leads to an improved bioassay platform...
- Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors
Badri P Maliwal
University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, 3500 Camp Bowie Boulevard, Fort Worth, Texas 76106, USA
J Biomed Opt 17:011006. 2012
....
- Fluorescent protein-labeled glucocorticoid receptor alpha isoform trafficking in cultured human trabecular meshwork cells
Adnan Dibas
Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA
Invest Ophthalmol Vis Sci 53:2938-50. 2012
..To characterize the roles of the cytoskeleton and heat shock protein 90 (HSP90) in steroid-induced glucocorticoid receptor alpha (GRα) translocation in cultured human trabecular meshwork cells...
