Rafal Fudala

Summary

Publications

  1. pmc Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys peptide
    Rafal Fudala
    Center of Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107, USA
    Curr Pharm Biotechnol 12:834-8. 2011
  2. pmc Fluorescence detection of hyaluronidase
    Rafal Fudala
    Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 104:473-7. 2011
  3. pmc Lifetime-based sensing of the hyaluronidase using fluorescein labeled hyaluronic acid
    Rafal Fudala
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 106:69-73. 2012
  4. pmc Long-lived bright red emitting azaoxa-triangulenium fluorophores
    Badri P Maliwal
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States of America
    PLoS ONE 8:e63043. 2013
  5. pmc Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAngulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC)
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Anal Bioanal Chem 405:4887-94. 2013
  6. pmc Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Anal Bioanal Chem 405:2065-75. 2013
  7. pmc Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 116:7-12. 2012
  8. ncbi FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer
    Rahul Chib
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Curr Pharm Biotechnol 14:470-4. 2013
  9. doi Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores
    Sangram Raut
    Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, Texas 76107, USA
    Nanoscale 6:385-91. 2014
  10. pmc Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain
    Krishna Midde
    Department of Cell Biology and Anatomy, University of North Texas, Health Science Center, Fort Worth, Texas 76107, USA
    J Biol Chem 288:7012-23. 2013

Collaborators

Detail Information

Publications14

  1. pmc Fluorescence detection of MMP-9. I. MMP-9 selectively cleaves Lys-Gly-Pro-Arg-Ser-Leu-Ser-Gly-Lys peptide
    Rafal Fudala
    Center of Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, UNTHSC, Fort Worth, TX 76107, USA
    Curr Pharm Biotechnol 12:834-8. 2011
    ..The control MMP-2 enzyme does not cause any changes in either 5-FAM or Cy5 fluorescence. We believe that our observation will help in early detection of elevated MMP-9 levels under disease conditions...
  2. pmc Fluorescence detection of hyaluronidase
    Rafal Fudala
    Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 104:473-7. 2011
    ..This in turn, can be utilized for the construction of a robust but reliable HA-ase sensing device...
  3. pmc Lifetime-based sensing of the hyaluronidase using fluorescein labeled hyaluronic acid
    Rafal Fudala
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 106:69-73. 2012
    ..We demonstrate that the calibration of the sensing response is the same for the average lifetime as for a single exponential decay approximation, which significantly simplifies the analysis of the sensing measurements...
  4. pmc Long-lived bright red emitting azaoxa-triangulenium fluorophores
    Badri P Maliwal
    Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, Texas, United States of America
    PLoS ONE 8:e63043. 2013
    ..The fluorescence lifetime facilitated gating of cellular nanosecond autofluorescence should be of considerable help in in vitro and in vivo applications...
  5. pmc Elimination of autofluorescence in fluorescence correlation spectroscopy using the AzaDiOxaTriAngulenium (ADOTA) fluorophore in combination with time-correlated single-photon counting (TCSPC)
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Anal Bioanal Chem 405:4887-94. 2013
    ..We then show that both time gating and FLCS may be used successfully with ADOTA-labeled HA to detect the presence of hyaluronidase, the overexpression of which has been observed in many types of cancer...
  6. pmc Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) fluorophore
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Anal Bioanal Chem 405:2065-75. 2013
    ..This time-gating eliminates over 96 % of autofluorescence. Analysis using a variable time-gate may enable quantitative determination of the bound probe without the contributions from the background...
  7. pmc Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy
    Ryan M Rich
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    J Photochem Photobiol B 116:7-12. 2012
    ..The method we present provides a sensitive measure of hyaluronidase activity and requires extremely small amounts of the HA substrate...
  8. ncbi FRET Based Ratio-Metric Sensing of Hyaluronidase in Synthetic Urine as a Biomarker for Bladder and Prostate Cancer
    Rahul Chib
    Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Curr Pharm Biotechnol 14:470-4. 2013
    ..Our fluorescence intensity results are also supported with time resolved fluorescence decay data. This assay can be used to develop a non-invasive technique for the detection of bladder and/or prostate cancer progression. ..
  9. doi Resonance energy transfer between fluorescent BSA protected Au nanoclusters and organic fluorophores
    Sangram Raut
    Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, 3500 Camp Bowie Blvd, Fort Worth, Texas 76107, USA
    Nanoscale 6:385-91. 2014
    ..Moreover, transferring energy from BSA Au clusters to Dy750 will result in a RET probe with a narrow emission spectrum and long lifetime component which can be utilized in imaging applications. ..
  10. pmc Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain
    Krishna Midde
    Department of Cell Biology and Anatomy, University of North Texas, Health Science Center, Fort Worth, Texas 76107, USA
    J Biol Chem 288:7012-23. 2013
    ..A simple theory was developed to account for this fact...
  11. pmc Properties of coatings on RFID p-Chips that support plasmonic fluorescence enhancement in bioassays
    Ryan Rich
    Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107, USA
    Anal Bioanal Chem 404:2223-31. 2012
    ..The analysis provides a possible mechanism by which the SIF/polymer-coated p-Chips allow a highly sensitive immunoassay and, as a result, leads to an improved bioassay platform...
  12. pmc Lenticular mitoprotection. Part A: Monitoring mitochondrial depolarization with JC-1 and artifactual fluorescence by the glycogen synthase kinase-3β inhibitor, SB216763
    Morgan M Brooks
    Department of Cell Biology and Anatomy, University of North Texas Health Science Center at Fort Worth, Fort Worth, TX, USA
    Mol Vis 19:1406-12. 2013
    ..We demonstrate the novel use of spectral deconvolution to negate the interfering contributing fluorescence by SB216763, thus allowing an unfettered analysis of the JC-1 dye to determine the mitochondrial membrane potential...
  13. pmc Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors
    Badri P Maliwal
    University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Center for Commercialization of Fluorescence Technologies, 3500 Camp Bowie Boulevard, Fort Worth, Texas 76106, USA
    J Biomed Opt 17:011006. 2012
    ....
  14. pmc Fluorescent protein-labeled glucocorticoid receptor alpha isoform trafficking in cultured human trabecular meshwork cells
    Adnan Dibas
    Department of Pharmacology and Neuroscience, University of North Texas Health Science Center at Fort Worth, 3500 Camp Bowie Boulevard, Fort Worth, TX 76107, USA
    Invest Ophthalmol Vis Sci 53:2938-50. 2012
    ..To characterize the roles of the cytoskeleton and heat shock protein 90 (HSP90) in steroid-induced glucocorticoid receptor alpha (GRα) translocation in cultured human trabecular meshwork cells...