Improved ethanol precipitation of DNARosa Fregel
Area de Genetica, Departamento de Parasitologia, Ecologia y Genetica, Facultad de Biologia, Universidad de La Laguna, Avenida Astrofísico Francisco Sánchez, Tenerife, Spain
Electrophoresis 31:1350-2. 2010
..It uses a mixture of 70% ethanol, 75 mM ammonium acetate and different concentrations of different carriers to perform DNA precipitation and washing in only one step...
- HaploSearch: a tool for haplotype-sequence two-way transformation
Rosa Fregel
Department of Genetics, Faculty of Biology, University of La Laguna, La Laguna, Tenerife, Spain
Mitochondrion 11:366-7. 2011
..Although designed for mitochondrial DNA, HaploSearch could be used with any kind of DNA type. HaploSearch program, detailed software instructions and example files are freely available on the web http://www.haplosite.com/haplosearch/...
Demographic history of Canary Islands male gene-pool: replacement of native lineages by EuropeanRosa Fregel
Department of Genetics, University of La Laguna, Avda Astrofísico Fco, Sánchez, La Laguna, 38271 Santa Cruz de Tenerife, Spain
BMC Evol Biol 9:181. 2009
....
- Temporal evolution of the ABO allele frequencies in the Canary Islands: the impact of the European colonization
Rosa Fregel
Department of Genetics, University of La Laguna, Avda Astrofísico Fco Sánchez, S N, 38271 La Laguna, Tenerife, Spain
Immunogenetics 61:603-10. 2009
..The historical sample shows a congruent intermediate position testifying already a strong European influence that would go in augment since then to present times, affecting all the islands with the important exception of La Gomera...
- The maternal aborigine colonization of La Palma (Canary Islands)
Rosa Fregel
Departamento de Genetica, Facultad de Biologia, Universidad de La Laguna, Tenerife, Spain
Eur J Hum Genet 17:1314-24. 2009
..Our data better fit to an island model with frequent migrations between islands...
- The loss of functional caspase-12 in Europe is a pre-neolithic event
Montserrat Hervella
Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, Leioa, Spain
PLoS ONE 7:e37022. 2012
..CONCLUSIONS/SIGNIFICANCE: We demonstrate that the loss of caspase-12 in Europe predates animal domestication and that consequently CASP12 loss is unlikely to be related to the impact of zoonotic infections transmitted by livestock...
- Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
Patricia Marrero
Grupo de Ecología y Evolución en Islas, IPNA CSIC, La Laguna E 38206, Tenerife, Islas Canarias, Spain
Biol Res 42:147-51. 2009
..Moreover, DNA of two bird species was identified from seeds expelled by regurgitation. Therefore, this method may be suitable for future ecological studies of birds, and other vertebrate groups...
Description of a simple multiplex PCR-SSCP method for AB0 genotyping and its application to the peopling of the Canary IslandsRosa Fregel
, , Universidad de La Laguna, Tenerife, Spain
Immunogenetics 57:572-8. 2005
..Similar to previous autosomal studies, admixture estimations point to a major Iberian contribution (82 +/- 0.5%) to the Canary Islands, although, in some islands as La Gomera, the NW African component raised to 62 +/- 4.3%...
- Mitochondrial DNA diversity in 17th-18th century remains from Tenerife (Canary Islands)
Nicole Maca-Meyer
, Universidad de La Laguna, 38271 La Laguna, Tenerife, Spain
Am J Phys Anthropol 127:418-26. 2005
..59%), has also decreased due to European input. Finally, the presence of Amerindian lineages (1.5%) reveals that the Canary Islands have also received genetic flow from America...
- Ancient DNA from hunter-gatherer and farmer groups from Northern Spain supports a random dispersion model for the Neolithic expansion into Europe
Montserrat Hervella
Department of Genetics, Physical Anthropology and Animal Physiology, University of the Basque Country, Bizkaia, Spain
PLoS ONE 7:e34417. 2012
..This random dispersion had a different impact on the various geographic regions, and thus contradicts the more simplistic total acculturation and replacement models proposed so far to explain Neolithisation...
