Jorg Tost


Country: France


  1. Tost J. A translational perspective on epigenetics in allergic diseases. J Allergy Clin Immunol. 2018;142:715-726 pubmed publisher
  2. Quigley D, Fiorito E, Nord S, Van Loo P, Alnæs G, Fleischer T, et al. The 5p12 breast cancer susceptibility locus affects MRPS30 expression in estrogen-receptor positive tumors. Mol Oncol. 2014;8:273-84 pubmed publisher
  3. Alnaes G, Ronneberg J, Kristensen V, Tost J. Heterogeneous DNA Methylation Patterns in the GSTP1 Promoter Lead to Discordant Results between Assay Technologies and Impede Its Implementation as Epigenetic Biomarkers in Breast Cancer. Genes (Basel). 2015;6:878-900 pubmed publisher
  4. Kernaleguen M, Daviaud C, Shen Y, Bonnet E, Renault V, Deleuze J, et al. Whole-Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution. Methods Mol Biol. 2018;1767:311-349 pubmed publisher
    ..We also provide a step-by-step description of the data analysis using publicly available bioinformatic tools. The described protocols have been successfully applied to different human samples and yield robust and reproducible results. ..
  5. Mauger F, How Kit A, Tost J. COLD-PCR Technologies in the Area of Personalized Medicine: Methodology and Applications. Mol Diagn Ther. 2017;21:269-283 pubmed publisher
    ..In this review, we describe in detail the different COLD-PCR approaches, highlighting their specificities, advantages, and inconveniences and demonstrating their use in different fields of biological and biomedical research. ..
  6. Andersen G, Hager H, Hansen L, Tost J. Improved reproducibility in genome-wide DNA methylation analysis for PAXgene-fixed samples compared with restored formalin-fixed and paraffin-embedded DNA. Anal Biochem. 2015;468:50-8 pubmed publisher
    ..In addition, DNA from PAXgene tissue can be directly used on the array without prior restoration, rendering the analytical process significantly more time- and cost-effective. ..
  7. Nielsen H, How Kit A, Guerin C, Castinetti F, Vollan H, De Micco C, et al. Copy number variations alter methylation and parallel IGF2 overexpression in adrenal tumors. Endocr Relat Cancer. 2015;22:953-67 pubmed publisher
    ..Our data provide conclusive evidence that loss of the maternal allele correlates with IGF2 overexpression in adrenal tumors and that hypermethylation of the H19 ICR is a consequence thereof. ..
  8. Bjaanæs M, Fleischer T, Halvorsen A, Daunay A, Busato F, Solberg S, et al. Genome-wide DNA methylation analyses in lung adenocarcinomas: Association with EGFR, KRAS and TP53 mutation status, gene expression and prognosis. Mol Oncol. 2016;10:330-43 pubmed publisher
    ..CpGs in the HOX B and HOX C gene clusters were represented in the prognostic signature. Methylation differences mirror biologically important features in the etiology of lung adenocarcinomas and influence prognosis. ..
  9. request reprint
    Tost J. Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns. Adv Exp Med Biol. 2016;945:343-430 pubmed

More Information


  1. Sefrioui D, Mauger F, Leclere L, Beaussire L, Di Fiore F, Deleuze J, et al. Comparison of the quantification of KRAS mutations by digital PCR and E-ice-COLD-PCR in circulating-cell-free DNA from metastatic colorectal cancer patients. Clin Chim Acta. 2017;465:1-4 pubmed publisher
    ..E-ice-COLD-PCR can accurately detect and quantify low-abundant mutations in ccfDNA and has a shorter time to results making it compatible with the requirements of analyses in a clinical setting without the loss of quantitative accuracy. ..
  2. Fogel O, Richard Miceli C, Tost J. Epigenetic Changes in Chronic Inflammatory Diseases. Adv Protein Chem Struct Biol. 2017;106:139-189 pubmed publisher
    ..We also discuss important parameters of meaningful epigenetic studies such as the use of well defined, disease-relevant cell populations, and elude on the potential future of engineering of the epigenome in inflammatory diseases. ..
  3. Eftang L, Klajic J, Kristensen V, Tost J, Esbensen Q, Blom G, et al. GFRA3 promoter methylation may be associated with decreased postoperative survival in gastric cancer. BMC Cancer. 2016;16:225 pubmed publisher
    ..The GFRA3 promoter region showed marked hypermethylation in almost all tumors, and its correlation with survival and other clinicopathological parameters may have important prognostic significance. ..
  4. Tost J, Hamzaoui H, Busato F, Neyret A, Mourah S, Dupont J, et al. Methylation of specific CpG sites in the P2 promoter of parathyroid hormone-related protein determines the invasive potential of breast cancer cell lines. Epigenetics. 2011;6:1035-46 pubmed publisher
    ..Methylation of the PTHrP P2 is a potential marker of breast cancer progression and might be used to evaluate the metastatic potential of breast tumors. ..
  5. Andersen G, Tost J. A Summary of the Biological Processes, Disease-Associated Changes, and Clinical Applications of DNA Methylation. Methods Mol Biol. 2018;1708:3-30 pubmed publisher
    ..As a stable nucleic acid-based modification that is technically easy to handle and which can be analyzed with great reproducibility and accuracy by different laboratories, DNA methylation is a promising biomarker for many applications. ..
  6. Daviaud C, Renault V, Mauger F, Deleuze J, Tost J. Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq Protocol. Methods Mol Biol. 2018;1708:83-104 pubmed publisher
    ..The described protocol has been successfully applied to different human samples as well as DNA extracted from plant tissues and yields robust and reproducible results. ..
  7. Busato F, Dejeux E, El abdalaoui H, Gut I, Tost J. Quantitative DNA Methylation Analysis at Single-Nucleotide Resolution by Pyrosequencing®. Methods Mol Biol. 2018;1708:427-445 pubmed publisher
    ..Advantages of the Pyrosequencing technology are the ease of its implementation, the high quality and the quantitative nature of the results, and its ability to identify differentially methylated positions in close proximity. ..