Caroline Smet

Summary

Affiliation: University of Lille 1
Location: Villeneuve dAscq, France
Summary:
Assistant Professor

Publications

  1. pmc SUMO-1 regulates the conformational dynamics of thymine-DNA Glycosylase regulatory domain and competes with its DNA binding activity
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, Université de Lille1 Université de Lille2 CNRS USR3078, Parc de la Haute Borne, 50 avenue de Halley, 59658 Villeneuve d Ascq, France
    BMC Biochem 12:4. 2011
  2. pmc SUMO-1 possesses DNA binding activity
    Sebastian Eilebrecht
    Institut des Hautes Etudes Scientifiques, 35 route de Chartres, 91440 Bures sur Yvette, France
    BMC Res Notes 3:146. 2010
  3. ncbi request reprint Pin1: a therapeutic target in Alzheimer neurodegeneration
    Malika Hamdane
    INSERM U422, IMPRT, Place de Verdun, Lille, France
    J Mol Neurosci 19:275-87. 2002
  4. doi request reprint Identification of O-GlcNAc sites within peptides of the Tau protein and their impact on phosphorylation
    Caroline Smet-Nocca
    Unite de Glycobiologie Structurale et Fonctionnelle, CNRS UMR8576, IFR 147, Université de Lille1, 59655 Villeneuve d Ascq cedex, France
    Mol Biosyst 7:1420-9. 2011
  5. doi request reprint NMR spectroscopy of the neuronal tau protein: normal function and implication in Alzheimer's disease
    Isabelle Landrieu
    CNRS UMR 8576 IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d Ascq cedex, France
    Biochem Soc Trans 38:1006-11. 2010
  6. doi request reprint NMR-based detection of acetylation sites in peptides
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, CNRS USR3078, Université de Lille1, Parc de la Haute Borne, 50 avenue de Halley, 59658 Villeneuve d Ascq Cedex, France
    J Pept Sci 16:414-23. 2010
  7. doi request reprint Structure and plasticity of the peptidyl-prolyl isomerase Par27 of Bordetella pertussis revealed by X-ray diffraction and small-angle X-ray scattering
    Bernard Clantin
    UMR8161 CNRS, Institut de Biologie de Lille, Institut Pasteur de Lille, Université de Lille 1 Université de Lille 2, 1 rue Calmette, 59021 Lille Cedex, France
    J Struct Biol 169:253-65. 2010
  8. doi request reprint The thymine-DNA glycosylase regulatory domain: residual structure and DNA binding
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, USR CNRS 3078, Universite de Lille 1, 1 rue du Professeur Calmette, 59021 Lille Cedex, France
    Biochemistry 47:6519-30. 2008
  9. doi request reprint The peptidyl-prolyl isomerase and chaperone Par27 of Bordetella pertussis as the prototype for a new group of parvulins
    Hélène Hodak
    INSERM U629, Lille, France
    J Mol Biol 376:414-26. 2008
  10. ncbi request reprint Molecular mechanisms of the phospho-dependent prolyl cis/trans isomerase Pin1
    G Lippens
    CNRS UMR 8576 Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1 59655, Villeneuve d Ascq, France
    FEBS J 274:5211-22. 2007

Collaborators

Detail Information

Publications19

  1. pmc SUMO-1 regulates the conformational dynamics of thymine-DNA Glycosylase regulatory domain and competes with its DNA binding activity
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, Université de Lille1 Université de Lille2 CNRS USR3078, Parc de la Haute Borne, 50 avenue de Halley, 59658 Villeneuve d Ascq, France
    BMC Biochem 12:4. 2011
    ..Regulation of TDG activity by SUMO-1 conjugation was shown to act on both functions. Furthermore, TDG can interact with SUMO-1 in a non-covalent manner...
  2. pmc SUMO-1 possesses DNA binding activity
    Sebastian Eilebrecht
    Institut des Hautes Etudes Scientifiques, 35 route de Chartres, 91440 Bures sur Yvette, France
    BMC Res Notes 3:146. 2010
    ..The precise molecular mechanisms by which SUMO conjugation and association act are, however, not understood...
  3. ncbi request reprint Pin1: a therapeutic target in Alzheimer neurodegeneration
    Malika Hamdane
    INSERM U422, IMPRT, Place de Verdun, Lille, France
    J Mol Neurosci 19:275-87. 2002
    ..The involvement of Pin1 in the G0/G1 transition in neurons points to its function as a good target for the development of new therapeutic strategies in neurodegenerative disorders...
  4. doi request reprint Identification of O-GlcNAc sites within peptides of the Tau protein and their impact on phosphorylation
    Caroline Smet-Nocca
    Unite de Glycobiologie Structurale et Fonctionnelle, CNRS UMR8576, IFR 147, Université de Lille1, 59655 Villeneuve d Ascq cedex, France
    Mol Biosyst 7:1420-9. 2011
    ..Reciprocally, S400 O-GlcNAcylation reduces S404 phosphorylation by the CDK2/cyclinA3 kinase and interrupts the GSK3β-mediated sequential phosphorylation process...
  5. doi request reprint NMR spectroscopy of the neuronal tau protein: normal function and implication in Alzheimer's disease
    Isabelle Landrieu
    CNRS UMR 8576 IFR 147, Lille 1 Science and Technology University, 59655 Villeneuve d Ascq cedex, France
    Biochem Soc Trans 38:1006-11. 2010
    ..The phosphorylated tau can next be use for functional assays or interaction assays with phospho-dependent protein partners, such as the prolyl cis-trans isomerase Pin1...
  6. doi request reprint NMR-based detection of acetylation sites in peptides
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, CNRS USR3078, Université de Lille1, Parc de la Haute Borne, 50 avenue de Halley, 59658 Villeneuve d Ascq Cedex, France
    J Pept Sci 16:414-23. 2010
    ..Furthermore, resonance changes upon acetylation are restricted to residues nearby the acetylation site, indicating that acetylation does not modify the overall peptide conformation...
  7. doi request reprint Structure and plasticity of the peptidyl-prolyl isomerase Par27 of Bordetella pertussis revealed by X-ray diffraction and small-angle X-ray scattering
    Bernard Clantin
    UMR8161 CNRS, Institut de Biologie de Lille, Institut Pasteur de Lille, Université de Lille 1 Université de Lille 2, 1 rue Calmette, 59021 Lille Cedex, France
    J Struct Biol 169:253-65. 2010
    ....
  8. doi request reprint The thymine-DNA glycosylase regulatory domain: residual structure and DNA binding
    Caroline Smet-Nocca
    Institut de Recherche Interdisciplinaire, USR CNRS 3078, Universite de Lille 1, 1 rue du Professeur Calmette, 59021 Lille Cedex, France
    Biochemistry 47:6519-30. 2008
    ..T mismatch processing activity of TDG...
  9. doi request reprint The peptidyl-prolyl isomerase and chaperone Par27 of Bordetella pertussis as the prototype for a new group of parvulins
    Hélène Hodak
    INSERM U629, Lille, France
    J Mol Biol 376:414-26. 2008
    ..Par27 displays both PPIase and chaperone activities in vitro. In vivo, Par27 might function as a general periplasmic chaperone in B. pertussis...
  10. ncbi request reprint Molecular mechanisms of the phospho-dependent prolyl cis/trans isomerase Pin1
    G Lippens
    CNRS UMR 8576 Unité de Glycobiologie Structurale et Fonctionnelle, Université des Sciences et Technologies de Lille 1 59655, Villeneuve d Ascq, France
    FEBS J 274:5211-22. 2007
    ..After considering the available work, it seems that further analysis is required to determine whether binding or catalysis is the primary mechanism through which Pin1 affects cell cycle progression...
  11. ncbi request reprint Exploring the molecular function of PIN1 by nuclear magnetic resonance
    Isabelle Landrieu
    CNRS UMR 8576, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    Curr Protein Pept Sci 7:179-94. 2006
    ..Finally, isomerization catalysis is thought to regulate the (de)phosphorylation of specific pThr/pSer-Pro motifs, exemplified by the stimulation of the dephosphorylation of pThr231 of Tau by the PP2A phosphatase...
  12. ncbi request reprint Studying the natively unfolded neuronal Tau protein by solution NMR spectroscopy
    Guy Lippens
    CNRS Université de Lille2 UMR 8525, Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP 245, 59019 Lille Cedex France
    Protein Pept Lett 13:235-46. 2006
    ..Preliminary NMR data on the fibers show that the assignment enables a precise mapping of the rigid core. Future NMR experiments should allow one to gain more insight into the conformational aspects of this intriguing protein...
  13. ncbi request reprint Control of protein-protein interactions: structure-based discovery of low molecular weight inhibitors of the interactions between Pin1 WW domain and phosphopeptides
    Caroline Smet
    CNRS University of Lille 2 UMR 8525, Institut Pasteur de Lille, Molecular Drug Discovery, 3 rue du Professeur Laguesse, 59000 Lille, France
    J Med Chem 48:4815-23. 2005
    ..Our structure-based strategy implies the synthesis of analogues of the Ac-Thr(PO(3)H(2))-Pro-NH(2) dipeptide and relies on high resolution NMR spectroscopy to accurately measure the affinity constants even in the high micromolar range...
  14. ncbi request reprint Regulation of Pin1 peptidyl-prolyl cis/trans isomerase activity by its WW binding module on a multi-phosphorylated peptide of Tau protein
    Caroline Smet
    UMR CNRS 8525 Structure et fonction des Biomolecules, Institut de Biologie de Lille, Institut Pasteur de Lille, BP 245 F 59019 Lille Cedex, France
    FEBS Lett 579:4159-64. 2005
    ..We therefore conclude that the WW domain can act as a negative regulator of enzymatic activity when multiple phosphorylation is present, thereby suggesting a subtle mechanism of its functional regulation...
  15. ncbi request reprint Accepting its random coil nature allows a partial NMR assignment of the neuronal Tau protein
    Caroline Smet
    CNRS Université de Lille 2, UMR 8525, Institut Pasteur de Lille, B P 245, 59019 Lille, France
    Chembiochem 5:1639-46. 2004
    ..The obtained assignments equally confirm the absence of regular secondary structure in the isolated protein...
  16. ncbi request reprint The peptidyl prolyl cis/trans-isomerase Pin1 recognizes the phospho-Thr212-Pro213 site on Tau
    Caroline Smet
    Institut de Biologie de Lille, Institut Pasteur de Lille, UMR CNRS 8525, BP 245, F 59019 Lille cedex, France
    Biochemistry 43:2032-40. 2004
    ....
  17. ncbi request reprint Proline-directed random-coil chemical shift values as a tool for the NMR assignment of the tau phosphorylation sites
    Guy Lippens
    CNRS Université de Lille 2 UMR 8525, Institut Pasteur de Lille, BP 245 59019 Lille Cedex, France
    Chembiochem 5:73-8. 2004
    ..This is a first step toward the study of the phosphorylation of Tau by NMR spectroscopy...
  18. ncbi request reprint Neurofibrillary degeneration of the Alzheimer-type: an alternate pathway to neuronal apoptosis?
    Malika Hamdane
    INSERM U422, Institut de Médecine Prédictive et Recherche Thérapeutique, Place de Verdun, F 59045 Lille Cedex, France
    Biochem Pharmacol 66:1619-25. 2003
    ....
  19. doi request reprint Unraveling a phosphorylation event in a folded protein by NMR spectroscopy: phosphorylation of the Pin1 WW domain by PKA
    Caroline Smet-Nocca
    Institut Fédératif de Recherches 147, CNRS UMR 8576, Université de Lille Nord de France, Villeneuve d Ascq, France
    J Biomol NMR 55:323-37. 2013
    ..Additionally, a structural model of the phospho-WW obtained by molecular dynamics simulation and energy minimization suggests that the phosphate moiety of phospho-S16 could compete with the phospho-substrate...