I J Schalk

Summary

Country: France

Publications

  1. ncbi request reprint Recycling of pyoverdin on the FpvA receptor after ferric pyoverdin uptake and dissociation in Pseudomonas aeruginosa
    Isabelle J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
    Biochemistry 41:1663-71. 2002
  2. ncbi request reprint Identification of residues of FpvA involved in the different steps of Pvd-Fe uptake in Pseudomonas aeruginosa
    Mirella Nader
    Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS and University Louis Pasteur, ESBS, F 67413 Illkirch, Strasbourg, France
    Biochemistry 46:11707-17. 2007
  3. ncbi request reprint Copurification of the FpvA ferric pyoverdin receptor of Pseudomonas aeruginosa with its iron-free ligand: implications for siderophore-mediated iron transport
    I J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Strasbourg, France
    Biochemistry 38:9357-65. 1999
  4. ncbi request reprint Pseudomonas fluorescens CHA0 produces enantio-pyochelin, the optical antipode of the Pseudomonas aeruginosa siderophore pyochelin
    Zeb A Youard
    Département de Microbiologie Fondamentale, Universite de Lausanne, Bâtiment Biophore, Quartier UNIL Sorge, CH 1015 Lausanne, Suisse
    J Biol Chem 282:35546-53. 2007
  5. doi request reprint Metal trafficking via siderophores in Gram-negative bacteria: specificities and characteristics of the pyoverdine pathway
    Isabelle J Schalk
    Université Strasbourg 1, Institut Gilbert Laustriat, CNRS UMR 7175, ESBS, 67412 Illkirch Cedex, France
    J Inorg Biochem 102:1159-69. 2008
  6. doi request reprint Structure-function relationships in the bifunctional ferrisiderophore FpvA receptor from Pseudomonas aeruginosa
    Isabelle J Schalk
    Département Récepteurs et Protéines Membranaires, Ecole Superieure de Biotechnologie de Strasbourg, Institut Gilbert Laustriat UMR CNRS Université Strasbourg I, Illkirch, France
    Biometals 22:671-8. 2009
  7. ncbi request reprint Recognition of iron-free siderophores by TonB-dependent iron transporters
    Isabelle J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
    Mol Microbiol 54:14-22. 2004
  8. ncbi request reprint Iron-free pyoverdin binds to its outer membrane receptor FpvA in Pseudomonas aeruginosa: a new mechanism for membrane iron transport
    I J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
    Mol Microbiol 39:351-60. 2001
  9. ncbi request reprint A new mechanism for membrane iron transport in Pseudomonas aeruginosa
    I J Schalk
    Département des Récepteurs et Protéines Membranaires UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
    Biochem Soc Trans 30:702-5. 2002
  10. ncbi request reprint The structure-activity relationship of ferric pyoverdine bound to its outer membrane transporter: implications for the mechanism of iron uptake
    V Schons
    UMR7100 CNRS, ESBS, Bld Sébastien Brant, F 67 412 Illkirch, Strasbourg, France
    Biochemistry 44:14069-79. 2005

Collaborators

Detail Information

Publications19

  1. ncbi request reprint Recycling of pyoverdin on the FpvA receptor after ferric pyoverdin uptake and dissociation in Pseudomonas aeruginosa
    Isabelle J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
    Biochemistry 41:1663-71. 2002
    ..The loading status of FpvA (PaA versus PaA-Fe) was shown to depend on the relative concentration of the two forms of pyoverdin in the growth medium...
  2. ncbi request reprint Identification of residues of FpvA involved in the different steps of Pvd-Fe uptake in Pseudomonas aeruginosa
    Mirella Nader
    Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS and University Louis Pasteur, ESBS, F 67413 Illkirch, Strasbourg, France
    Biochemistry 46:11707-17. 2007
    ..Surprisingly, the S603C mutation in loop L7 increased the amount of Pvd-Fe transported. Our results suggest that W434 (L4), S603 (L7), and G701 (L9) are involved in the mechanism of Pvd-Fe uptake...
  3. ncbi request reprint Copurification of the FpvA ferric pyoverdin receptor of Pseudomonas aeruginosa with its iron-free ligand: implications for siderophore-mediated iron transport
    I J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Strasbourg, France
    Biochemistry 38:9357-65. 1999
    ..This suggests a similar function for FpvA. The mechanism of iron transport in P. aeruginosa via the pyoverdin pathway is discussed in the light of all these new findings...
  4. ncbi request reprint Pseudomonas fluorescens CHA0 produces enantio-pyochelin, the optical antipode of the Pseudomonas aeruginosa siderophore pyochelin
    Zeb A Youard
    Département de Microbiologie Fondamentale, Universite de Lausanne, Bâtiment Biophore, Quartier UNIL Sorge, CH 1015 Lausanne, Suisse
    J Biol Chem 282:35546-53. 2007
    ..aeruginosa siderophore pyochelin. Enantio-pyochelin was chemically synthesized and confirmed to be active in CHA0. Its potential biosynthetic pathway in CHA0 is discussed...
  5. doi request reprint Metal trafficking via siderophores in Gram-negative bacteria: specificities and characteristics of the pyoverdine pathway
    Isabelle J Schalk
    Université Strasbourg 1, Institut Gilbert Laustriat, CNRS UMR 7175, ESBS, 67412 Illkirch Cedex, France
    J Inorg Biochem 102:1159-69. 2008
    ..These data are discussed and compared with the mechanisms of siderophore-Fe uptake reported for other Gram-negative bacteria...
  6. doi request reprint Structure-function relationships in the bifunctional ferrisiderophore FpvA receptor from Pseudomonas aeruginosa
    Isabelle J Schalk
    Département Récepteurs et Protéines Membranaires, Ecole Superieure de Biotechnologie de Strasbourg, Institut Gilbert Laustriat UMR CNRS Université Strasbourg I, Illkirch, France
    Biometals 22:671-8. 2009
    ..It is likely that conclusions drawn for FpvA will be relevant to other TonB-dependent ferrisiderophore transport and signalling proteins...
  7. ncbi request reprint Recognition of iron-free siderophores by TonB-dependent iron transporters
    Isabelle J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
    Mol Microbiol 54:14-22. 2004
    ....
  8. ncbi request reprint Iron-free pyoverdin binds to its outer membrane receptor FpvA in Pseudomonas aeruginosa: a new mechanism for membrane iron transport
    I J Schalk
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
    Mol Microbiol 39:351-60. 2001
    ..The mechanism of iron uptake in P. aeruginosa via the pyoverdin pathway is discussed in view of these new findings...
  9. ncbi request reprint A new mechanism for membrane iron transport in Pseudomonas aeruginosa
    I J Schalk
    Département des Récepteurs et Protéines Membranaires UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
    Biochem Soc Trans 30:702-5. 2002
    ..One of the key features of this process is the binding of iron-free pyoverdin to the outer membrane receptor FpvA in conditions of iron deficiency...
  10. ncbi request reprint The structure-activity relationship of ferric pyoverdine bound to its outer membrane transporter: implications for the mechanism of iron uptake
    V Schons
    UMR7100 CNRS, ESBS, Bld Sébastien Brant, F 67 412 Illkirch, Strasbourg, France
    Biochemistry 44:14069-79. 2005
    ..All these data have serious implications for the specificity and mechanism of PvdI-mediated iron transport in P. aeruginosa...
  11. ncbi request reprint Real time fluorescent resonance energy transfer visualization of ferric pyoverdine uptake in Pseudomonas aeruginosa. A role for ferrous iron
    Jason Greenwald
    Métaux et Microorganismes Chimie, Biologie, et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS Université Louis Pasteur, ESBS, Boulevard Sebastien Brant, F 67413 Illkirch, Strasbourg, France
    J Biol Chem 282:2987-95. 2007
    ..Finally, in vivo fluorescence microscopy revealed patches of PvdI, suggesting that uptake occurs via macromolecular assemblies on the cell surface...
  12. pmc Pyoverdine-mediated iron uptake in Pseudomonas aeruginosa: the Tat system is required for PvdN but not for FpvA transport
    Rome Voulhoux
    IBSM LISM, CNRS, Marseille Cedex, France
    J Bacteriol 188:3317-23. 2006
    ..Our results further support the idea that a critical step of the Pvd biogenesis pathway involving PvdN occurs at the periplasmic side of the cytoplasmic membrane...
  13. ncbi request reprint Bacterial siderophores: the solution stoichiometry and coordination of the Fe(III) complexes of pyochelin and related compounds
    Chiu Fou Tseng
    Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 115, Taiwan, Republic of China
    J Biol Inorg Chem 11:419-32. 2006
    ..These results are consistent with the role of pyochelin in the uptake of iron by the FptA receptor in the outer membrane of Pseudomonas aeruginosa and in several gram-negative bacteria...
  14. ncbi request reprint Binding properties of pyochelin and structurally related molecules to FptA of Pseudomonas aeruginosa
    Gaëtan L A Mislin
    Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS, Université Louis Pasteur Strasbourg 1, ESBS, Bld Sébastien Brant, F 67413 Illkirch, Strasbourg, France
    J Mol Biol 357:1437-48. 2006
    ..All these findings improve our understanding of specificity of the interaction between FptA and its siderophore...
  15. ncbi request reprint Binding of iron-free siderophore, a common feature of siderophore outer membrane transporters of Escherichia coli and Pseudomonas aeruginosa
    Françoise Hoegy
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67413 Illkirch, Strasbourg, France
    J Biol Chem 280:20222-30. 2005
    ..These findings improved our understanding of the iron uptake mechanism via siderophores in Gram-negative bacteria...
  16. ncbi request reprint The crystal structure of the pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa at 3.6 angstroms resolution
    David Cobessi
    Département Récepteurs et Protéines Membranaires, UPR9050 CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, 67412 Illkirch, France
    J Mol Biol 347:121-34. 2005
    ..coli TonB-dependent transporters reveals a high structural homology and suggests that a common sensing mechanism exists for the iron-loading status in all bacterial iron siderophore transporters...
  17. ncbi request reprint The binding mechanism of pyoverdin with the outer membrane receptor FpvA in Pseudomonas aeruginosa is dependent on its iron-loaded status
    Emilie Clément
    Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brandt, BP 10412, F 67413 Illkirch, Strasbourg, France
    Biochemistry 43:7954-65. 2004
    ..Apparently, under iron-limited conditions, TonB activates the FpvA receptor, resulting in a fast release of iron-free Pvd and generating an unloaded FpvA receptor, competent for binding extracellular Pvd-Fe...
  18. ncbi request reprint The interaction between pyoverdin and its outer membrane receptor in Pseudomonas aeruginosa leads to different conformers: a time-resolved fluorescence study
    Nicolas Folschweiller
    ESBS, Ecole Superieure de Biotechnologie de Strasbourg, UPR CNRS 9050, Bld Sébastien Brant, 67400 Illkirch, France
    Biochemistry 41:14591-601. 2002
    ..In addition, the Förster resonance energy transfer kinetics from FpvA tryptophan residues to the PaA chromophore differs from one complex to the other, revealing differences in one or more of the donor-acceptor topologies...
  19. ncbi request reprint Synthesis of pyochelin-norfloxacin conjugates
    Freddy Rivault
    Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat CNRS Université Louis Pasteur Strasbourg I, ESBS, Boulevard Sebastien Brant, F 67400 Illkirch, France
    Bioorg Med Chem Lett 17:640-4. 2007
    ....