Research Topics
| I J SchalkSummaryCountry: France Publications
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Publications
Recycling of pyoverdin on the FpvA receptor after ferric pyoverdin uptake and dissociation in Pseudomonas aeruginosaIsabelle J Schalk
Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
Biochemistry 41:1663-71. 2002..The loading status of FpvA (PaA versus PaA-Fe) was shown to depend on the relative concentration of the two forms of pyoverdin in the growth medium...- Identification of residues of FpvA involved in the different steps of Pvd-Fe uptake in Pseudomonas aeruginosaMirella Nader
Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS and University Louis Pasteur, ESBS, F 67413 Illkirch, Strasbourg, France
Biochemistry 46:11707-17. 2007..Surprisingly, the S603C mutation in loop L7 increased the amount of Pvd-Fe transported. Our results suggest that W434 (L4), S603 (L7), and G701 (L9) are involved in the mechanism of Pvd-Fe uptake... - Copurification of the FpvA ferric pyoverdin receptor of Pseudomonas aeruginosa with its iron-free ligand: implications for siderophore-mediated iron transportI J Schalk
Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Strasbourg, France
Biochemistry 38:9357-65. 1999..This suggests a similar function for FpvA. The mechanism of iron transport in P. aeruginosa via the pyoverdin pathway is discussed in the light of all these new findings... - Pseudomonas fluorescens CHA0 produces enantio-pyochelin, the optical antipode of the Pseudomonas aeruginosa siderophore pyochelinZeb A Youard
Département de Microbiologie Fondamentale, Universite de Lausanne, Bâtiment Biophore, Quartier UNIL Sorge, CH 1015 Lausanne, Suisse
J Biol Chem 282:35546-53. 2007..aeruginosa siderophore pyochelin. Enantio-pyochelin was chemically synthesized and confirmed to be active in CHA0. Its potential biosynthetic pathway in CHA0 is discussed... 
Metal trafficking via siderophores in Gram-negative bacteria: specificities and characteristics of the pyoverdine pathwayIsabelle J Schalk
Université Strasbourg 1, Institut Gilbert Laustriat, CNRS UMR 7175, ESBS, 67412 Illkirch Cedex, France
J Inorg Biochem 102:1159-69. 2008..These data are discussed and compared with the mechanisms of siderophore-Fe uptake reported for other Gram-negative bacteria...- Structure-function relationships in the bifunctional ferrisiderophore FpvA receptor from Pseudomonas aeruginosaIsabelle J Schalk
Département Récepteurs et Protéines Membranaires, Ecole Superieure de Biotechnologie de Strasbourg, Institut Gilbert Laustriat UMR CNRS Université Strasbourg I, Illkirch, France
Biometals 22:671-8. 2009..It is likely that conclusions drawn for FpvA will be relevant to other TonB-dependent ferrisiderophore transport and signalling proteins... - Recognition of iron-free siderophores by TonB-dependent iron transportersIsabelle J Schalk
Département des Récepteurs et Protéines Membranaires, UPR 9050, CNRS, ESBS, Bld Sébastien Brant, F 67 400 Illkirch, Strasbourg, France
Mol Microbiol 54:14-22. 2004.... - Iron-free pyoverdin binds to its outer membrane receptor FpvA in Pseudomonas aeruginosa: a new mechanism for membrane iron transportI J Schalk
Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
Mol Microbiol 39:351-60. 2001..The mechanism of iron uptake in P. aeruginosa via the pyoverdin pathway is discussed in view of these new findings... - A new mechanism for membrane iron transport in Pseudomonas aeruginosaI J Schalk
Département des Récepteurs et Protéines Membranaires UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67400 Illkirch, Strasbourg, France
Biochem Soc Trans 30:702-5. 2002..One of the key features of this process is the binding of iron-free pyoverdin to the outer membrane receptor FpvA in conditions of iron deficiency... - The structure-activity relationship of ferric pyoverdine bound to its outer membrane transporter: implications for the mechanism of iron uptakeV Schons
UMR7100 CNRS, ESBS, , F-67 412 Illkirch, Strasbourg, France
Biochemistry 44:14069-79. 2005..All these data have serious implications for the specificity and mechanism of PvdI-mediated iron transport in P. aeruginosa... - Real time fluorescent resonance energy transfer visualization of ferric pyoverdine uptake in Pseudomonas aeruginosa. A role for ferrous ironJason Greenwald
Métaux et Microorganismes Chimie, Biologie, et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS Université Louis Pasteur, ESBS, Boulevard Sebastien Brant, F 67413 Illkirch, Strasbourg, France
J Biol Chem 282:2987-95. 2007..Finally, in vivo fluorescence microscopy revealed patches of PvdI, suggesting that uptake occurs via macromolecular assemblies on the cell surface... 
Pyoverdine-mediated iron uptake in Pseudomonas aeruginosa: the Tat system is required for PvdN but not for FpvA transportRome Voulhoux
IBSM LISM, CNRS, Marseille Cedex, France
J Bacteriol 188:3317-23. 2006..Our results further support the idea that a critical step of the Pvd biogenesis pathway involving PvdN occurs at the periplasmic side of the cytoplasmic membrane...- Bacterial siderophores: the solution stoichiometry and coordination of the Fe(III) complexes of pyochelin and related compoundsChiu-Fou Tseng
Institute of Chemistry, Academia Sinica, 128 Academia Road Section 2, Nankang, Taipei 115, Taiwan, Republic of China
J Biol Inorg Chem 11:419-32. 2006..These results are consistent with the role of pyochelin in the uptake of iron by the FptA receptor in the outer membrane of Pseudomonas aeruginosa and in several gram-negative bacteria... - Binding properties of pyochelin and structurally related molecules to FptA of Pseudomonas aeruginosaGaëtan L A Mislin
Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS, Université Louis Pasteur Strasbourg 1, ESBS, Bld Sébastien Brant, F 67413 Illkirch, Strasbourg, France
J Mol Biol 357:1437-48. 2006..All these findings improve our understanding of specificity of the interaction between FptA and its siderophore... - Binding of iron-free siderophore, a common feature of siderophore outer membrane transporters of Escherichia coli and Pseudomonas aeruginosaFrançoise Hoegy
Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brant, F 67413 Illkirch, Strasbourg, France
J Biol Chem 280:20222-30. 2005..These findings improved our understanding of the iron uptake mechanism via siderophores in Gram-negative bacteria... - The crystal structure of the pyoverdine outer membrane receptor FpvA from Pseudomonas aeruginosa at 3.6 angstroms resolutionDavid Cobessi
Département Récepteurs et Protéines Membranaires, UPR9050 CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, 67412 Illkirch, France
J Mol Biol 347:121-34. 2005..coli TonB-dependent transporters reveals a high structural homology and suggests that a common sensing mechanism exists for the iron-loading status in all bacterial iron siderophore transporters... - The binding mechanism of pyoverdin with the outer membrane receptor FpvA in Pseudomonas aeruginosa is dependent on its iron-loaded statusEmilie Clément
Département des Récepteurs et Protéines Membranaires, UPR 9050 CNRS, ESBS, Bld Sébastien Brandt, BP 10412, F 67413 Illkirch, Strasbourg, France
Biochemistry 43:7954-65. 2004..Apparently, under iron-limited conditions, TonB activates the FpvA receptor, resulting in a fast release of iron-free Pvd and generating an unloaded FpvA receptor, competent for binding extracellular Pvd-Fe... - The interaction between pyoverdin and its outer membrane receptor in Pseudomonas aeruginosa leads to different conformers: a time-resolved fluorescence studyNicolas Folschweiller
ESBS, , UPR CNRS 9050, , 67400 Illkirch, France
Biochemistry 41:14591-601. 2002..In addition, the Förster resonance energy transfer kinetics from FpvA tryptophan residues to the PaA chromophore differs from one complex to the other, revealing differences in one or more of the donor-acceptor topologies... - Synthesis of pyochelin-norfloxacin conjugatesFreddy Rivault
Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat CNRS Université Louis Pasteur Strasbourg I, ESBS, Boulevard Sebastien Brant, F 67400 Illkirch, France
Bioorg Med Chem Lett 17:640-4. 2007....