Michel Salzet

Summary

Country: France

Publications

  1. ncbi request reprint Innate immunity in lophotrochozoans: the annelids
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Curr Pharm Des 12:3043-50. 2006
  2. pmc Construction of a medicinal leech transcriptome database and its application to the identification of leech homologs of neural and innate immune genes
    Eduardo R Macagno
    Division of Biological Sciences, University of California, San Diego, CA, USA
    BMC Genomics 11:407. 2010
  3. ncbi request reprint Theromin, a novel leech thrombin inhibitor
    M Salzet
    Laboratoire d Endocrinologie des Annélides, Unité Propre de la Recherche Supérieure Associée au CNRS 8017 CNRS, SN3, Université des Sciences et Technologie de Lille, F 59655 Villeneuve d Ascq Cedex, France
    J Biol Chem 275:30774-80. 2000
  4. ncbi request reprint Chromacin-like peptide in leeches
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq Cedex, France
    Neuro Endocrinol Lett 24:227-32. 2003
  5. ncbi request reprint Neuroimmunology of opioids from invertebrates to human
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, UPRESA CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Neuro Endocrinol Lett 22:467-74. 2001
  6. ncbi request reprint Cellular localization of a renin-like enzyme in leeches
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, ESA CNRS 8017, SN3, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Neuro Endocrinol Lett 22:453-9. 2001
  7. ncbi request reprint The angiotensin system elements in invertebrates
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A 8017 CNRS, SN3, Université des Sciences et Technologie de Lille, F 59655, Villeneuve d Ascq Cedex, France
    Brain Res Brain Res Rev 36:35-45. 2001
  8. ncbi request reprint Anticoagulants and inhibitors of platelet aggregation derived from leeches
    M Salzet
    Laboratoire d Endocrinologie et Immunité des Annélides, UPRESA CNRS 8017, Universite des Sciences et Technologies de Lille, SN3, F 59655 Cedex, Villeneuve d Ascq, France
    FEBS Lett 492:187-92. 2001
  9. ncbi request reprint Involvement of pro-enkephalin-derived peptides in immunity
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRESA CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq Cedex, France
    Dev Comp Immunol 25:177-85. 2001
  10. ncbi request reprint Molecular crosstalk in host-parasite relationships: schistosome- and leech-host interactions
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, Cedex, France
    Parasitol Today 16:536-40. 2000

Collaborators

Detail Information

Publications78

  1. ncbi request reprint Innate immunity in lophotrochozoans: the annelids
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Curr Pharm Des 12:3043-50. 2006
    ..In the present review, we will emphasize the different immunodefense strategies that adaptation has taken during the course of evolution of two classes of annelids i.e. oligochaetes and achaetes...
  2. pmc Construction of a medicinal leech transcriptome database and its application to the identification of leech homologs of neural and innate immune genes
    Eduardo R Macagno
    Division of Biological Sciences, University of California, San Diego, CA, USA
    BMC Genomics 11:407. 2010
    ..To begin to address this problem, we constructed whole embryo and adult central nervous system (CNS) EST libraries and created a clustered sequence database of the Hirudo transcriptome that is available to the scientific community...
  3. ncbi request reprint Theromin, a novel leech thrombin inhibitor
    M Salzet
    Laboratoire d Endocrinologie des Annélides, Unité Propre de la Recherche Supérieure Associée au CNRS 8017 CNRS, SN3, Université des Sciences et Technologie de Lille, F 59655 Villeneuve d Ascq Cedex, France
    J Biol Chem 275:30774-80. 2000
    ..In summary, this potent thrombin inhibitor promises to have high biomedical significance...
  4. ncbi request reprint Chromacin-like peptide in leeches
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq Cedex, France
    Neuro Endocrinol Lett 24:227-32. 2003
    ..The level of this peptide decreased significantly 4 hours after LPS exposure. This report is the first discovery of a chromogranin derived like peptide in invertebrates...
  5. ncbi request reprint Neuroimmunology of opioids from invertebrates to human
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, UPRESA CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Neuro Endocrinol Lett 22:467-74. 2001
    ..This demonstrates that the equilibrium between the stimulation and the inhibition of the immune response has evolved sooner that it can be thought...
  6. ncbi request reprint Cellular localization of a renin-like enzyme in leeches
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, ESA CNRS 8017, SN3, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Neuro Endocrinol Lett 22:453-9. 2001
    ..The purpose of this study was to localize in leeches the renin-like enzyme previously characterized as well as the leech angiotensin-converting like enzyme (ACE)...
  7. ncbi request reprint The angiotensin system elements in invertebrates
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A 8017 CNRS, SN3, Université des Sciences et Technologie de Lille, F 59655, Villeneuve d Ascq Cedex, France
    Brain Res Brain Res Rev 36:35-45. 2001
    ..As the elements of this hormone-enzymatic system also exist in invertebrates, it appears that the RAS originated very early in evolution...
  8. ncbi request reprint Anticoagulants and inhibitors of platelet aggregation derived from leeches
    M Salzet
    Laboratoire d Endocrinologie et Immunité des Annélides, UPRESA CNRS 8017, Universite des Sciences et Technologies de Lille, SN3, F 59655 Cedex, Villeneuve d Ascq, France
    FEBS Lett 492:187-92. 2001
    ..Moreover, clinical trials confirm their potential to treat cardiovascular diseases...
  9. ncbi request reprint Involvement of pro-enkephalin-derived peptides in immunity
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRESA CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq Cedex, France
    Dev Comp Immunol 25:177-85. 2001
    ..The focus of this review is to describe how the pro-enkephalin derived peptides participate in immune processes...
  10. ncbi request reprint Molecular crosstalk in host-parasite relationships: schistosome- and leech-host interactions
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, Cedex, France
    Parasitol Today 16:536-40. 2000
    ..Here, Michel Salzet, André Capron and George Stefano review recent work on the interaction of common signaling mechanisms in ..
  11. ncbi request reprint Neuropeptide-derived antimicrobial peptides from invertebrates for biomedical applications
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, IFR18, SN3, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    Curr Med Chem 12:3055-61. 2005
    ..e. dipeptides and anionic peptides been given. Moreover, these molecules are also present in human and may serve in the innate immune response as an important survival strategy...
  12. ncbi request reprint PLGamide characterization and role in osmoregulation in leech brain
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    Brain Res Mol Brain Res 76:161-9. 2000
    ..PLGamide did not bind to mu-alkaloid opioid receptors and did not stimulate NO release...
  13. ncbi request reprint Invertebrate molecular neuroimmune processes
    M Salzet
    Laboratoire d Endocrinologie des Annélides, UPRES A CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Cedex, Villeneuve d Ascq, France
    Brain Res Brain Res Rev 34:69-79. 2000
    ..Parasites use these immunosuppressors with magnificence in their crosstalk with their host...
  14. pmc Interaction of HmC1q with leech microglial cells: involvement of C1qBP-related molecule in the induction of cell chemotaxis
    Muriel Tahtouh
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée EA4550, Microglial activation group, Universite Lille Nord de France, Universite Lille 1, IFR 147, Batiment SN3, 59655 Villeneuve d Ascq, France
    J Neuroinflammation 9:37. 2012
    ..In this study, we evaluated the chemotactic activities of a recombinant form of HmC1q and its interaction with a newly characterized leech C1qBP that acts as its potential ligand...
  15. doi request reprint Deciphering the immune function and regulation by a TLR of the cytokine EMAPII in the lesioned central nervous system using a leech model
    David Schikorski
    Laboratoire de Neuroimmunologie des Annelides, FRE Centre National de la Recherche Scientifique 2933, Groupe Signaux de Danger, Voies de Signalisation et Effecteurs, Universite de Lille 1, 59655 Villeneuve d Ascq, France
    J Immunol 183:7119-28. 2009
    ....
  16. pmc Cathepsin L and cystatin B gene expression discriminates immune coelomic cells in the leech Theromyzon tessulatum
    Christophe Lefebvre
    Universite de Lille 1, Laboratoire de Neuroimmunologie des Annelides, FRE 2933 Centre National de Recherche Scientifique CNRS, IFR147, Batiment SN3, Université de Lille1, 59655 Villeneuve d Ascq cedex, France
    Dev Comp Immunol 32:795-807. 2008
    ..Finally, results evidenced that cathepsin L immunopositive granular amoebocytes are chemoattracted to the site of injury and phagocyte bacteria...
  17. doi request reprint The C-terminal fragment of the immunoproteasome PA28S (Reg alpha) as an early diagnosis and tumor-relapse biomarker: evidence from mass spectrometry profiling
    Rémi Longuespée
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée, Université Nord de France, EA 4550, Universite de Lille 1, Cite Scientifique, 59650 Villeneuve d Ascq, France
    Histochem Cell Biol 138:141-54. 2012
    ..5 % in stage I and 81.25 % ± 1.06 in stage III-IV of OVC. The correlation value for the different OVC stages is p < 0.001 of 0.998. Taken together, this report constitutes the first evidence of a novel OVC-specific marker...
  18. ncbi request reprint Up-regulation of neurohemerythrin expression in the central nervous system of the medicinal leech, Hirudo medicinalis, following septic injury
    David Vergote
    Laboratoire de Neuroimmunologie des Annelides, UMR 8017 CNRS, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    J Biol Chem 279:43828-37. 2004
    ..We consider potential roles of neurohemerythrin, associated with its ability to bind oxygen and iron, in the innate immune response of the leech nervous system to bacterial invasion...
  19. doi request reprint Characterization and immune function of two intracellular sensors, HmTLR1 and HmNLR, in the injured CNS of an invertebrate
    Virginie Cuvillier-Hot
    Laboratoire de Neuroimmunologie et Neurochimie Evolutive, Université Nord de France, CNRS FRE 3249, Cite Scientifique, 59655 Villeneuve d Ascq, France
    Dev Comp Immunol 35:214-26. 2011
    ..This work constitutes the first demonstration in an invertebrate of (i) an intracellular TLR and (ii) a cytosolic PRR related to the NLR family...
  20. pmc Multiple changes in peptide and lipid expression associated with regeneration in the nervous system of the medicinal leech
    Céline Mériaux
    Universite Lille Nord de France, Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée FABMS, EA 4550, Universite Lille 1, Villeneuve d Ascq, France
    PLoS ONE 6:e18359. 2011
    ..Moreover, the hypothesis that neural regeneration involves some molecular factors initially employed during embryonic neural development is tested...
  21. pmc Characterization of the first non-insect invertebrate functional angiotensin-converting enzyme (ACE): leech TtACE resembles the N-domain of mammalian ACE
    Guillaume Rivière
    Laboratoire de Neuroendocrinologie du Developpement, UPRES EA 2701, Bat SN4 2e étage, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Biochem J 382:565-73. 2004
    ..Although the precise roles and endogenous substrates of TtACE remain to be identified, characterization of this ancestral peptidase will help to clarify its physiological roles in non-insect invertebrate species...
  22. doi request reprint Evidence for a novel chemotactic C1q domain-containing factor in the leech nerve cord
    Muriel Tahtouh
    Université de Lille 1 USTL, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 2933, IFR 147, F59655 Villeneuve d Ascq, France
    Mol Immunol 46:523-31. 2009
    ..Its chemoattractant activity on microglia highlights a new investigation field leading to better understand leech CNS repair mechanisms...
  23. doi request reprint A homologous form of human interleukin 16 is implicated in microglia recruitment following nervous system injury in leech Hirudo medicinalis
    Françoise Croq
    Universite Lille Nord de France, Universite Lille 1, Laboratoire de Neuroimmunologie des Annelides, Centre National de la Recherche Scientifique, FRE 3249, IFR 147, F59655 Villeneuve d Ascq, France
    Glia 58:1649-62. 2010
    ..The ability of HmIL-16 to recruit microglial cells to sites of CNS injury suggests a role for HmIL-16 in the crosstalk between neurons and microglia in the leech CNS repair...
  24. ncbi request reprint Molecular characterization of two novel antibacterial peptides inducible upon bacterial challenge in an annelid, the leech Theromyzon tessulatum
    Aurélie Tasiemski
    Centre National de la Recherche Scientifique, Laboratoire de Neuroimmunologie UMR 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    J Biol Chem 279:30973-82. 2004
    ....
  25. doi request reprint Ovarian cancer molecular pathology
    Rémi Longuespée
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée, Université Nord de France, EA 4550, Universite de Lille 1, Cite Scientifique, 59650 Villeneuve d Ascq, France
    Cancer Metastasis Rev 31:713-32. 2012
    ..In the present review, we will focus on such proteomic strategies in regards to OVC signaling pathways, OVC development and escape from the immune response...
  26. doi request reprint Morphological and functional characterization of leech circulating blood cells: role in immunity and neural repair
    Céline Boidin-Wichlacz
    Laboratoire de Neuroimmunologie et Neurochimie Evolutive, CNRS, FRE3249, Universite de Lille 1, Villeneuve d Ascq, France
    Cell Mol Life Sci 69:1717-31. 2012
    ..Understanding the basic function of the peripheral circulating cells and their interactions with lesioned CNS in the leech would allow us to acquire insights into the complexity of the neuroimmune response of the injured mammalian brain...
  27. ncbi request reprint New developments in MALDI imaging for pathology proteomic studies
    Maxence Wisztorski
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, Equipe Imagerie MALDI, Cite Scientifique, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, Quebec, J1H 5N4, Canada
    Curr Pharm Des 13:3317-24. 2007
    ..Disease/health states will thus be closely molecularly monitored at protein and nucleic acids levels, with a single technique. Taken together, MALDI imaging will become a key tool for pathology proteomic studies...
  28. pmc MALDI imaging mass spectrometry: state of the art technology in clinical proteomics
    Julien Franck
    MALDI Imaging Team, Laboratoire de Neuroimmunologie des Annelides, IFR 147, CNR FRE 2933, University of Lille1, 59655 Villeneuve d Ascq, France
    Mol Cell Proteomics 8:2023-33. 2009
    ....
  29. doi request reprint Specific MALDI-MSI: Tag-Mass
    Jonathan Stauber
    Laboratoire de Neuroimmunologie et Neurochimie Evolutives, FRE CNRS 3249, MALDI Imaging Team, Universite Lille Nord de France, Universite Lille 1, Villeneuve d Ascq, France
    Methods Mol Biol 656:339-61. 2010
    ..Here, we describe how we used this technique to detect huge proteins and mRNA by MALDI imaging in rat brain and in a model for regeneration; the leech...
  30. pmc Microbial challenge promotes the regenerative process of the injured central nervous system of the medicinal leech by inducing the synthesis of antimicrobial peptides in neurons and microglia
    David Schikorski
    Centre National de la Recherche Scientifique FRE2933, Laboratoire de Neuroimmunologie des Annelides, Universite de Lille 1, Villeneuve d Ascq, France
    J Immunol 181:1083-95. 2008
    ..Thus, the leech CNS appears as an excellent model for studying the implication of immune molecules in neural repair...
  31. doi request reprint Microproteomics by liquid extraction surface analysis: Application to FFPE tissue to study the fimbria region of tubo-ovarian cancer
    Maxence Wisztorski
    Laboratoire LSMBFA, Spectrométrie de Masse Biologique, Fondamentale et Appliquée, EA 4550, Universite Lille 1, Cite Scientifique, Villeneuve d Ascq, France
    Proteomics Clin Appl 7:234-40. 2013
    ..This method combines advantages of direct tissue MS analysis keeping histological information and conventional proteomics approaches for confident identification of proteins in complex sample...
  32. ncbi request reprint Presence of chromogranin-derived antimicrobial peptides in plasma during coronary artery bypass surgery and evidence of an immune origin of these peptides
    Aurélie Tasiemski
    Laboratoire de Neuroimmunologie des Annelides, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Blood 100:553-9. 2002
    ..Because IL-6 release is known to be strongly enhanced during CPB, we suggest a possible relationship between IL-6 and the increased level of secretolytin in patients undergoing CPB...
  33. doi request reprint MITICS (MALDI Imaging Team Imaging Computing System): a new open source mass spectrometry imaging software
    Olivia Jardin-Mathé
    University of Lille 1, CNRS FRE 2933, MALDI Imaging Team, Laboratoire de Neuroimmunologie des Annelides, Villeneuve d Ascq, France
    J Proteomics 71:332-45. 2008
    ..It also offers image reconstruction base either on the maximum peak intensity or the peak area. Image reconstruction is possible for single ions but also by summing signals of different ions. MITICS was validated on biological cases...
  34. doi request reprint Improving tissue preparation for matrix-assisted laser desorption ionization mass spectrometry imaging. Part 1: using microspotting
    Julien Franck
    Université de Lille1, CNRS, Team, Laboratoire de Neuroimmunologie des Annelides, MALDI Imaging, F 59655 Villeneuve d Ascq Cedex, France
    Anal Chem 81:8193-202. 2009
    ..We have shown that microspotting is an interesting approach for on tissue digestion. Peptides, proteins, and lipids were studied under this specific preparation strategy to improve imaging performances for this class of molecules...
  35. ncbi request reprint Antibacterial properties of hemerythrin of the sand worm Nereis diversicolor
    Laurence Deloffre
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, IFR 118, SN3, Universite des Sciences et Technologies de Lille, F 59655 Villeneuve d Ascq Cedex, France
    Neuro Endocrinol Lett 24:39-45. 2003
    ..To investigate the immune defense of the annelid Nereis diversicolor and the key role of a oxygen-binding protein, the metalloprotein MPII animals were subjected to bacteria infection...
  36. pmc Transcriptomic analysis in the leech Theromyzon tessulatum: involvement of cystatin B in innate immunity
    Christophe Lefebvre
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    Biochem J 380:617-25. 2004
    ....
  37. doi request reprint Epithelial-mesenchymal transition in ovarian cancer
    Daniele Vergara
    CNFS FRE, IFR, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Cancer Lett 291:59-66. 2010
    ..Alterations in these cellular pathways candidate them as useful target for ovarian cancer treatment...
  38. ncbi request reprint Elements of angiotensin system are involved in leeches and mollusks immune response modulation
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, ESA CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Cedex, Villeneuve d Ascq, France
    Brain Res Mol Brain Res 94:137-47. 2001
    ..This is the first report in invertebrates and of a co-action between cytokines like substances and neuropeptides in an immune process and the involvement of the RAS in modulation of the immune response...
  39. doi request reprint Ionic matrices pre-spotted matrix-assisted laser desorption/ionization plates for patient maker following in course of treatment, drug titration, and MALDI mass spectrometry imaging
    David Bonnel
    Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée FABMS, Universite Lille 1, 59655 Villeneuve d Ascq, France
    Anal Biochem 434:187-98. 2013
    ..We show that these pre-spotted ionic matrices plates can be used for relative drug quantification, high mass protein detection, and MALDI mass spectrometry imaging...
  40. doi request reprint MALDI direct analysis and imaging of frozen versus FFPE tissues: what strategy for which sample?
    Maxence Wisztorski
    Laboratoire de Neuroimmunologie et Neurochimie Evolutives, FRE CNRS 3249, MALDI Imaging Team, Universite Lille Nord de France, Universite Lille 1, Villeneuve d Ascq Cedex, France
    Methods Mol Biol 656:303-22. 2010
    ..We present methods and protocols for FFPE samples. These strategies have proven to be valuable for all tested FFPE samples and have opened archived tissues from hospital banks to MALDI-MSI...
  41. doi request reprint Molecular MALDI imaging: an emerging technology for neuroscience studies
    Maxence Wisztorski
    Equipe Imagerie MALDI, Cite Scientifique, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    Dev Neurobiol 68:845-58. 2008
    ....
  42. doi request reprint Multivariate analyses for biomarkers hunting and validation through on-tissue bottom-up or in-source decay in MALDI-MSI: application to prostate cancer
    David Bonnel
    MALDI Imaging Team, Laboratoire de Spectrométrie de Masse Biologique Fondamentale et Appliquée FABMS, EA 4550, Université Nord de France, Universite Lille 1, Villeneuve d Ascq, France
    Anal Bioanal Chem 401:149-65. 2011
    ..We present an example from a prostate cancer study using formalin-fixed paraffin-embedded tissue...
  43. doi request reprint On tissue protein identification improvement by N-terminal peptide derivatization
    Julien Franck
    Laboratoire de Neuroimmunologie et Neurochimie Evolutives, FRE CNRS 3249, MALDI Imaging Team, Universite Lille Nord de France, Universite Lille 1, Villeneuve d Ascq, France
    Methods Mol Biol 656:323-38. 2010
    ..This provides a complete strategy for protein identification in a bottom-up MALDI-MSI approach and opens the way for novel biomarker identification...
  44. ncbi request reprint Angiotensin-converting enzyme inhibition studies by natural leech inhibitors by capillary electrophoresis and competition assay
    Laurence Deloffre
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    Eur J Biochem 271:2101-6. 2004
    ..These results showed that capillary zonal electrophoresis is a useful technique for following enzymatic processes with small amounts of products and constitutes the first evidence of a natural ACE inhibitor in invertebrates...
  45. ncbi request reprint Oxytocin-like peptide: a novel epitope colocalized with the FMRFamide-like peptide in the supernumerary neurons of the sex segmental ganglia of leeches--morphological and biochemical characterization; putative anti-diuretic function
    M Salzet
    , ERS CNRS 20, , Villeneuve d Ascq, France
    Brain Res 601:173-84. 1993
    ..tessulatum, indicated that MIF (the epitope found in the sex SG) and sex SG have the same anti-diuretic effect on the leeches injected. These results pointed to an anti-diuretic role of the leech OT-like substance...
  46. doi request reprint Proteomic analyses of serous and endometrioid epithelial ovarian cancers - cases studies - molecular insights of a possible histological etiology of serous ovarian cancer
    Rémi Longuespée
    Université Nord de France, LSMBFA, MALDI Imaging Team, EA 4550, Universite de Lille 1, SIRIC ONCOLILLE, Cite Scientifique, Villeneuve d Ascq, France
    Proteomics Clin Appl 7:337-54. 2013
    ..In this study, MALDI MS profiling technology was used to provide molecular insights regarding these potentially different mechanisms...
  47. ncbi request reprint [Endocrine markers of cellular immunity: defining the endocrine phenotype]
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, IFR 118, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq, France
    J Soc Biol 197:97-101. 2003
    ....
  48. ncbi request reprint Leech immunity: from brain to peripheral responses
    Aurélie Tasiemski
    Universite Lille Nord de France, CNRS, Laboratoire de Neuroimmunologie et Neurochimie Evolutives, Universite Lille 1, France
    Adv Exp Med Biol 708:80-104. 2010
    ..These points that the neuroimmune and immune responses evolved sooner than can be expected...
  49. ncbi request reprint Leech immunocytes contain proopiomelanocortin: nitric oxide mediates hemolymph proopiomelanocortin processing
    M Salzet
    Centre de Biologie Cellulaire, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    J Immunol 159:5400-11. 1997
    ..Leech alpha-MSH had the same activity as authentic alpha-MSH in two bioasssay systems. Taken together, the study demonstrates that POMC is present in invertebrates and its immunoregulatory actions have been conserved during evolution...
  50. ncbi request reprint Prodynorphin in invertebrates
    M Salzet
    Laboratoire de Phylogénie Moléculaire des Annélides EA DRED 1027, Universite des Sciences et Technologies de Lille, France
    Brain Res Mol Brain Res 52:46-52. 1997
    ..Leech prodynorphin is distinguished from that found in mammals in that the N-terminus is shorter. This report constitutes the first complete biochemical characterization of a prodynorphin in invertebrates...
  51. ncbi request reprint Leech egg-laying-like hormone: structure, neuronal distribution and phylogeny
    M Salzet
    Centre de Biologie Cellulaire, Laboratoire de Phylogénie Moléculaire des Annélides, EA DRED 1027, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Brain Res Mol Brain Res 49:211-21. 1997
    ..This finding constitutes the first biochemical characterization of an egg-laying hormone in other invertebrates than mollusks...
  52. ncbi request reprint A renin-like enzyme in the leech Theromyzon tessulatum
    M Salzet
    Centre de Biologie Cellulaire, Laboratoire de Phylogénie Moléculaire des Annélides, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Mol Cell Endocrinol 131:1-8. 1997
    ..This is the first biochemical characterization of a renin-like enzyme in invertebrates and non-mammalian vertebrates...
  53. ncbi request reprint Isolation and characterization of a leech neuropeptide in rat brains: coupling to nitric oxide release in leech, rat and human tissues
    M Salzet
    Centre de Biologie Cellulaire, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Brain Res Mol Brain Res 55:173-9. 1998
    ..The study demonstrates that LORF, and its function in relation to NO release, has been conserved over more than 400 million years of evolution...
  54. ncbi request reprint Biochemical evidence of the sodium influx stimulating related peptide in the brain of the leech Theromyzon tessulatum
    M Salzet
    Centre de Biologie Cellulaire, EA DRED 1027, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Neurosci Lett 213:161-4. 1996
    ..Moreover, results of injections of anti-SISP (10-19) preabsorbed or not with its homologous antigen to Theromyzon tessulatum confirmed the SIS-like substance involvement in osmoregulation by an anti-diuretic function...
  55. doi request reprint Tissue imaging using MALDI-MS: a new frontier of histopathology proteomics
    Isabelle Fournier
    Laboratoire de Neurobiologie des Annélides, FRE CNRS 2933, MALDI Imaging Team, University of Lille 1, F 59655 Villeneuve d Ascq Cedex, France
    Expert Rev Proteomics 5:413-24. 2008
    ..In this review, MALDI imaging technology is presented as well as applications of such technology in cancer or neurodegenerative diseases...
  56. ncbi request reprint Cellular localization of a chromogranin B-like derived peptides in leeches
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, IFR 118, Université des Sciences et Technologies de Lille 59650 Villeneuve d Ascq, France
    Neuro Endocrinol Lett 23:209-12. 2002
    ..Finally, the whole of the data showed for the first time the presence in leeches of CGB like derived peptides...
  57. ncbi request reprint [MALDI imaging: a new technology to discover and validate new biomarkers]
    Maxence Wisztorski
    Laboratoire de Neuro Immunologie des Annélides, FRE CNRS 2933, Equipe Imagerie MALDI, Batiment SN3, Cite Scientifique, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    Med Sci (Paris) 23:31-6. 2007
    ....
  58. ncbi request reprint Annelid neuroimmune system
    Christophe Lefebvre
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Curr Pharm Des 9:149-58. 2003
    ..e. implicating prohormone convertase enzymes...
  59. ncbi request reprint Direct analysis of neuropeptides by in situ MALDI-TOF mass spectrometry in the rat brain
    Isabelle Fournier
    Laboratoire de Neuroimmunologie des Annelides, UMR CNRS 8017, SN3, Universite des Sciences et Technologies de Lille, F 59655 Villeneuve d Ascq Cedex, France
    Neuro Endocrinol Lett 24:9-14. 2003
    ..MALDI-MS on brain slides is a novel complementary technique for neurobiologists and endocrinologists in order to investigate the dynamic and regional distribution of neuropeptides during physiological events...
  60. ncbi request reprint The endocannabinoid system in invertebrates
    M Salzet
    Laboratoire de Neuroimmunologie des Annelides, LIMR CNRS 8017, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Prostaglandins Leukot Essent Fatty Acids 66:353-61. 2002
    ..We propose that since this system worked so well, it was retained during evolution, and that invertebrates can serve as a model to study endogenous cannabinoid signaling...
  61. ncbi request reprint Specific MALDI imaging and profiling for biomarker hunting and validation: fragment of the 11S proteasome activator complex, Reg alpha fragment, is a new potential ovary cancer biomarker
    Remi Lemaire
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, MALDI Imaging Team, Cite Scientifique, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    J Proteome Res 6:4127-34. 2007
    ..Taken together these studies reflect that direct tissue analysis and specific MALDI imaging strategies facilitate biomarker hunting and validation which can be named pathological proteomics...
  62. ncbi request reprint Cloning, expression and pharmacological characterization of a vasopressin-related receptor in an annelid, the leech Theromyzon tessulatum
    A Levoye
    Laboratoire de Neuroimmunologie des Annelides, UMR 8017 CNRS, SN3, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    J Endocrinol 184:277-89. 2005
    ..This study describes the characterization of a novel AVP/OT superfamily receptor in annelids, which are considered the most distant group of coelomate metazoans possessing a functional AVP/OT-related endocrine system...
  63. ncbi request reprint MALDI-MS direct tissue analysis of proteins: Improving signal sensitivity using organic treatments
    R Lemaire
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, Bat SN3, 1 étage, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    Anal Chem 78:7145-53. 2006
    ....
  64. ncbi request reprint Structural characterization of osmoregulator peptides from the brain of the leech Theromyzon tessulatum: IPEPYVWD and IPEPYVWD-amide
    M Salzet
    Centre de Biologie Cellulalaire, EA DRED 1027, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Brain Res Mol Brain Res 43:301-10. 1996
    ..These peptides differ from peptides to the oxytocin/vasopressin family and are unique in the animal kingdom. Confirmation of their action on the hydric balance and their distribution in the CNS were presented...
  65. ncbi request reprint Leech thrombin inhibitors
    Michel Salzet
    Laboratoire de Neuroimmunologie des Annelides, ESA CNRS 8017, SN3, IFR 17 INSERM, Universite des Sciences et Technologies de Lille, Villeneuve, 59655 Villeneuve d Ascq cedex, France
    Curr Pharm Des 8:493-503. 2002
    ..In this review, we focus our attention on thrombin inhitors...
  66. ncbi request reprint Direct analysis and MALDI imaging of formalin-fixed, paraffin-embedded tissue sections
    R Lemaire
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, MALDI Imaging Team, Cite Scientifique, Universite des Sciences et Technologies de Lille, 59650 Villeneuve d Ascq, France
    J Proteome Res 6:1295-305. 2007
    ..These combined approaches, along with FFPE tissue analysis provide access to massive amounts of archived samples in the clinical pathology setting...
  67. ncbi request reprint Solid ionic matrixes for direct tissue analysis and MALDI imaging
    R Lemaire
    Laboratoire de Neuroimmunologie des Annelides, Equipe Imagerie MALDI, UMR CNRS 8017, Batiment SN3, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Anal Chem 78:809-19. 2006
    ..These results give a new outlook on peptide tissue profiling by MS, characterization of compounds from tissue slices, and MALDI-MS high-quality imaging...
  68. pmc Tag-mass: specific molecular imaging of transcriptome and proteome by mass spectrometry based on photocleavable tag
    R Lemaire
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, MALDI Imaging Team, Universite des Sciences et Technologies de Lille, 59655 Villeneuve d Ascq cedex, France
    J Proteome Res 6:2057-67. 2007
    ..Tag-Mass development is the key for a rapid, sensitive, and accurate approach to correlate levels of expression of different mRNA or proteins in diseases...
  69. doi request reprint On-tissue N-terminal peptide derivatizations for enhancing protein identification in MALDI mass spectrometric imaging strategies
    J Franck
    MALDI Imaging Team, Laboratoire de Neuroimmunologie des Annelides, CNRS FRE 2933, Université de Lille1, IFR 147, F 59655 Villeneuve d Ascq Cedex, France
    Anal Chem 81:8305-17. 2009
    ..This derivatization can be performed at room temperature, which greatly facilitates the automation of the process. The TMPP derivatization therefore yields an advantageous new generation of derivatives suited for use in tissue...
  70. doi request reprint MALDI imaging of formalin-fixed paraffin-embedded tissues: application to model animals of Parkinson disease for biomarker hunting
    J Stauber
    Universite des Sciences et Technologies de Lille, FRE CNRS 2933, MALDI Imaging Team, Laboratoire de Neuroimmunologie des Annelides, IFR 147, Cite Scientifique, 59655 Villeneuve d Ascq cedex, France
    J Proteome Res 7:969-78. 2008
    ..The access to archived FFPE tissue using MALDI profiling and imaging opens a whole new area in clinical studies and biomarker discovery from hospital biopsy libraries...
  71. ncbi request reprint Lipopolysaccharide mediated regulation of neuroendocrine associated proprotein convertases and neuropeptide precursor processing in the rat spleen
    Guillaume Lansac
    Departement de Pharmacologie, Faculte de Medecine, Universite de Sherbrooke, Sherbrooke, Quebec, Canada, J1H 5N4
    J Neuroimmunol 171:57-71. 2006
    ..Collectively, these data clearly implicate PCs in immune responses, both innate and acquired...
  72. ncbi request reprint Proteome modifications of the medicinal leech nervous system under bacterial challenge
    David Vergote
    Laboratoire de Neuroimmunologie des Annelides, FRE CNRS 2933, Universite des Sciences et Technologies de Lille, Villeneuve d Ascq, France
    Proteomics 6:4817-25. 2006
    ..Moreover, several of these proteins are specifically expressed in glial cells, suggesting an important role for glial cells in the immune response of the leech nervous system, similar to what has been observed in vertebrates...
  73. ncbi request reprint The nervous system and innate immunity: the neuropeptide connection
    Kim A Brogden
    Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52252, USA
    Nat Immunol 6:558-64. 2005
    ..As examples, we have chosen five well described, locally produced neuropeptides that may serve a previously unrecognized, direct antimicrobial, innate immune function in four anatomical sites...
  74. ncbi request reprint Presence and regulation of the endocannabinoid system in human dendritic cells
    Isabel Matias
    Endocannabinoid Research Group, Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Comprensorio Olivetti, Pozzuoli Napoli, Italy
    Eur J Biochem 269:3771-8. 2002
    ..These findings demonstrate for the first time that the endogenous cannabinoid system is present in human dendritic cells and can be regulated by cell activation...
  75. ncbi request reprint The neuroendocrine phenotype, cellular plasticity, and the search for genetic switches: redefining the diffuse neuroendocrine system
    Robert Day
    Department de Pharmacologie, Institut de Pharmacologie de Sherbrooke, Universite de Sherbrooke, 3001, 12e Avenue Nord, Sherbrooke, Quebec, Canada J1H5N4
    Neuro Endocrinol Lett 23:447-51. 2002
    ....
  76. ncbi request reprint Antimicrobial peptides from animals: focus on invertebrates
    Jacopo Vizioli
    Trends Pharmacol Sci 23:494-6. 2002
  77. ncbi request reprint Trypsin and chymotrypsin inhibitors in insects and gut leeches
    Liliane Schoofs
    Laboratory of Developmental Physiology and Molecular Biology, K U Leuven, Naamsestraat 59, Leuven, B 3000 Leuven, Belgium
    Curr Pharm Des 8:483-91. 2002
    ..Also other invertebrates such as insects, which evolved from the annelids, harvest potential interesting molecules, such as serine protease inhibitors that can be exploited by the medical industry...
  78. ncbi request reprint Antimicrobial peptides versus parasitic infections?
    Jacopo Vizioli
    Trends Parasitol 18:475-6. 2002
    ..In vitro antiparasitic assays suggest that antimicrobial peptides could represent a powerful tool for the development of novel drugs to fight the parasite in the vertebrate host, or to complement current therapeutic strategies...