- Hepatitis C virus ultrastructure and morphogenesisPhilippe Roingeard
Laboratoire de Biologie Cellulaire et Laboratoire de Virologie, Faculté de Médecine ed Centre Hospitalier Universitaire, Tours, France
Biol Cell 96:103-8. 2004..The HCV-LP model opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions, which may make it possible to establish the long-awaited in vitro culture system for HCV...
- [The lipid droplet: a new organelle?]Philippe Roingeard
INSERM U966, Laboratoire de Biologie Cellulaire, université François Rabelais et CHU de Tours, 10, boulevard Tonnelle, 37032 Tours, France
Med Sci (Paris) 29:495-500. 2013..Nevertheless, they represent important cellular structures for which dysfunctions may lead to human diseases such as lypodystrophies or neurodegenerative diseases...
- Hepatitis C virus diversity and hepatic steatosisP Roingeard
INSERM U966, Université François Rabelais and CHRU de Tours, Tours, France
J Viral Hepat 20:77-84. 2013..It is therefore difficult to distinguish between viral and metabolic steatosis unambiguously, and host and viral factors are probably involved in both HCV genotype 3 and nongenotype 3 steatosis...
- Centrosomal pre-integration latency of HIV-1 in quiescent cellsAlessia Zamborlini
CNRS UMR7151, Universite Paris 7, Hopital Saint Louis, Paris, France
Retrovirology 4:63. 2007..Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate...
- The birth and life of lipid droplets: learning from the hepatitis C virusPhilippe Roingeard
Laboratoire de Biologie Cellulaire, INSERM U966, Faculte de Medecine, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours, France
Biol Cell 103:223-31. 2011..Recent studies in this model have raised the hypothesis that the HCV core protein induces the redistribution of LDs through the regression and regeneration of these organelles in specific intracellular domains...
- Viral detection by electron microscopy: past, present and futurePhilippe Roingeard
INSERM ERI 19 and Electron Microscopy Facility, Université François Rabelais and CHRU de Tours, Tours, France
Biol Cell 100:491-501. 2008..Recent examples of different viral assembly models illustrate the value of TEM for improving our understanding of virus-cell interactions...
- Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopyPhilippe Roingeard
Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, INSERM ERI 19, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours Cedex, France
Histochem Cell Biol 130:561-6. 2008..This may have important implications for our understanding of the complex relationship between HCV and lipids and may make easier to dissect out the HCV life cycle...
- Hepatitis C virus core protein, lipid droplets and steatosisP Roingeard
INSERM ERI 19, Université François Rabelais and CHRU de Tours, Tours, France
J Viral Hepat 15:157-64. 2008..The association of HCV core protein with lipid droplets seems to play a central role in HCV pathogenesis and morphogenesis, suggesting that virus-induced steatosis may be essential for the viral life cycle...
- Antigenic properties of recombinant envelope glycoproteins derived from T-cell-line-adapted isolates or primary human immunodeficiency virus isolates and their relationship to immunogenicityD Brand
Unité de virologie, Universite Francois Rabelais, Tours, France
Virology 271:350-62. 2000..Thus, it may be difficult to design an effective vaccine based on native recombinant PI envelopes...
- The genotype 3-specific hepatitis C virus core protein residue phenylalanine 164 increases steatosis in an in vitro cellular modelC Hourioux
Universite Francois Rabelais, INSERM ERI 19 and CHRU de Tours, 10 boulevard Tonnelle, F 37032 Tours cedex, France
Gut 56:1302-8. 2007..An in vitro cellular model was used to investigate whether an HCV core protein with residues specific to genotype 3 increased this phenomenon...
- Core protein cleavage by signal peptide peptidase is required for hepatitis C virus-like particle assemblyMalika Ait-Goughoulte
INSERM ESPRI 3856, IFR 136, Universite Francois Rabelais, Faculté de Médecine and CHU, 10 boulevard Tonnelle, 37032 Tours, France
J Gen Virol 87:855-60. 2006..These data shed light on the HCV core residues involved in SPP cleavage and suggest that this cleavage is essential for HCV assembly...
- Hepatitis C virus-like particle morphogenesisEmmanuelle Blanchard
Laboratoire de Virologie, E3M EA3250, IFR 82, Faculte de Medecine et Centre Hospitalier Universitaire, Tours, France
J Virol 76:4073-9. 2002..This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions...
- The cell biology of hepatitis C virus (HCV) lipid addiction: molecular mechanisms and its potential importance in the clinicEric Piver
INSERM U966, Universite Francois Rabelais de Tours, Faculte de Medecine, 10 Bd Tonnellé, 37000 Tours, France piver
Int J Biochem Cell Biol 42:869-79. 2010..In the present review we summarise the current knowledge establishing the interplay between the molecular features of HCV replication, the cellular lipid biology and the lipid profiles observed in the serum of infected patients...
- Morphogenesis of hepatitis B virus and its subviral envelope particlesRomuald Patient
Universite Francois Rabelais, INSERM U 966, Tours, France
Cell Microbiol 11:1561-70. 2009..However, recent data clearly suggest that two different processes, including constitutive Golgi pathway or cellular machinery that generates internal vesicles of multivesicular bodies (MVB), independently form these two viral entities...
- Ultrastructural and quantitative analysis of the lipid droplet clustering induced by hepatitis C virus core proteinMarion Depla
INSERM U966, Faculte de Medecine, Universite Francois Rabelais, CHRU de Tours, 10 boulevard Tonnelle, 37032, Tours Cedex, France
Cell Mol Life Sci 67:3151-61. 2010..These observations provide useful information for further studies of the mechanisms involved in HCV-induced steatosis...
- Hepatitis B virus subviral envelope particle morphogenesis and intracellular traffickingRomuald Patient
Universite Francois Rabelais, INSERM ERI 19, Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, 10 boulevard Tonnelle, F 37032 Tours cedex, France
J Virol 81:3842-51. 2007..These findings shed light on an important step in the HBV infectious cycle, as the intracellular accumulation of HBV subviral filaments may be directly linked to viral pathogenesis...
- Ultrastructural and biochemical analyses of hepatitis C virus-associated host cell membranesPauline Ferraris
INSERM U966, Université François Rabelais and CHRU de Tours, France
J Gen Virol 91:2230-7. 2010..More detailed characterization of these virus-cell interactions may facilitate the development of new treatments active against HCV and other RNA viruses that are dependent on newly synthesized cellular membranes for replication...
- Impact of natural polymorphism within the gp41 cytoplasmic tail of human immunodeficiency virus type 1 on the intracellular distribution of envelope glycoproteins and viral assemblyMarie Lambelé
Universite Francois Rabelais, Tours, France
J Virol 81:125-40. 2007..Thus, polymorphisms in primary HIV-1 gp41 CTs at the quasispecies or subtype level can influence the intracellular distribution of Env, its incorporation into virions, and viral replication capacity...
- Vesicular stomatitis virus glycoprotein: a transducing coat for SFV-based RNA vectorsFabien Dorange
D3M, The Vector Group, Faculte de Medecine de Tours, 2 Bd Tonnelle, 37000 Tours, France
J Gene Med 6:1014-22. 2004..However, despite several modifications in the production process, a risk of contamination with replication-competent, or partially recombined, virus has remained...
- Chimeric hepatitis B and C viruses envelope proteins can form subviral particles: implications for the design of new vaccine strategiesRomuald Patient
INSERM U966, Université François Rabelais and CHRU de Tours, France
N Biotechnol 25:226-34. 2009....
- The transmembrane protein of HIV-1 primary isolates modulates cell surface expression of their envelope glycoproteinsS Lebigot
Unité de virologie, Département de Microbiologie Médicale et Moléculaire, EA3250 IFR 273, Universite Francois Rabelais, 37032 Tours, France
Virology 290:136-42. 2001..Identification of this signal may be important for the design of PI envelope-derived immunogens and may increase our understanding of the mechanisms by which HIV-1 escapes from the immune system...
- Endogenous virus and hepatitis C virus-like particle budding in BHK-21 cellsEmmanuelle Blanchard
J Virol 77:3888-9; author reply 3889. 2003
- Antiviral effect of alpha-glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particlesCynthia Chapel
INSERM U271, Laboratoire des virus hépatiques et pathologies associées, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France
J Gen Virol 87:861-71. 2006..These alpha-glucosidase inhibitors may prove to be useful molecules to fight HCV infection in combination protocols...
- Hepatitis C virus-like particle budding: role of the core protein and importance of its Asp111Emmanuelle Blanchard
Laboratoire de Virologie, Faculte de Medecine et Centre Hospitalier Universitaire, 2 bis Boulevard Tonnelle, 37032 Tours, France
J Virol 77:10131-8. 2003..In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis...
- Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell linesBoyan Grigorov
LaboRetro Unité de virologie humaine INSERM U758, IFR128 Biosciences Lyon Gerland, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69 364 Lyon, France
J Mol Biol 359:848-62. 2006....
- [Hepatitis C virus and lipid droplets]Philippe Roingeard
Med Sci (Paris) 23:461-3. 2007
- Vacuolization in hepatitis B virus-infected hepatocytesPhilippe Roingeard
Hepatology 37:1223-4; author reply 1224. 2003
- Core protein domains involved in hepatitis C virus-like particle assembly and budding at the endoplasmic reticulum membraneChristophe Hourioux
Universite Francois Rabelais de Tours, INSERM ERI 19 Tours, France
Cell Microbiol 9:1014-27. 2007....
- Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virusYves Rouille
CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
J Virol 80:2832-41. 2006..In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle...
- Centrosomal latency of incoming foamy viruses in resting cellsJacqueline Lehmann-Che
Universite Paris 7, Paris, France
PLoS Pathog 3:e74. 2007..Incoming foamy retroviruses can stably persist at the centrosome, awaiting cell stimulation to initiate capsid cleavage, nuclear import, and viral gene expression...
- [Revisiting HIV-1 assembly]Antoine Corbin
Laborétro Unité de virologie humaine, INSERM U758, IFR128, Ecole Normale Superieure de Lyon, 46 allee d Italie, Lyon, France
Med Sci (Paris) 24:49-55. 2008..Release of virions in the form of bursts would be triggered during cell-cell interactions, through a specialized structure called the virological synapse...
- Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitorsCynthia Chapel
INSERM, U871, Universite Lyon 1, et IFR62 Laennec, Lyon, France
J Gen Virol 88:1133-43. 2007..These properties suggest the potential usefulness of DNJ derivatives in combating HCV infection...