P Roingeard

Summary

Country: France

Publications

  1. ncbi request reprint Hepatitis C virus ultrastructure and morphogenesis
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire et Laboratoire de Virologie, Faculté de Médecine ed Centre Hospitalier Universitaire, Tours, France
    Biol Cell 96:103-8. 2004
  2. ncbi request reprint Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles
    Julien Burlaud-Gaillard
    Plate Forme RIO des Microscopies, PPF ASB, Université François Rabelais and CHRU de Tours, Tours, France
    PLoS ONE 9:e93573. 2014
  3. pmc Viral sequence variation in chronic carriers of hepatitis C virus has a low impact on liver steatosis
    Marion Depla
    INSERM U966, Université François Rabelais and CHRU de Tours, Tours, France
    PLoS ONE 7:e33749. 2012
  4. pmc Nuclear lipid droplets identified by electron microscopy of serial sections
    Rustem Uzbekov
    Plateforme des Microscopies, Université François Rabelais and CHRU de Tours, Tours, France
    BMC Res Notes 6:386. 2013
  5. doi request reprint [The lipid droplet: a new organelle?]
    Philippe Roingeard
    INSERM U966, Laboratoire de Biologie Cellulaire, université François Rabelais et CHU de Tours, 10, boulevard Tonnelle, 37032 Tours, France
    Med Sci (Paris) 29:495-500. 2013
  6. doi request reprint Hepatitis C virus diversity and hepatic steatosis
    P Roingeard
    INSERM U966, Université François Rabelais and CHRU de Tours, Tours, France
    J Viral Hepat 20:77-84. 2013
  7. pmc Centrosomal pre-integration latency of HIV-1 in quiescent cells
    Alessia Zamborlini
    CNRS UMR7151, Universite Paris 7, Hopital Saint Louis, Paris, France
    Retrovirology 4:63. 2007
  8. ncbi request reprint Hepatitis C virus core protein, lipid droplets and steatosis
    P Roingeard
    INSERM ERI 19, Université François Rabelais and CHRU de Tours, Tours, France
    J Viral Hepat 15:157-64. 2008
  9. doi request reprint Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopy
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, INSERM ERI 19, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours Cedex, France
    Histochem Cell Biol 130:561-6. 2008
  10. doi request reprint Viral detection by electron microscopy: past, present and future
    Philippe Roingeard
    INSERM ERI 19 and Electron Microscopy Facility, Université François Rabelais and CHRU de Tours, Tours, France
    Biol Cell 100:491-501. 2008

Collaborators

  • D Brand
  • S Lebigot
  • B Verrier
  • Catherine Gaudy-Graffin
  • Jean Christophe Meunier
  • Beatrice Labrosse
  • Camille Sureau
  • Francis Barin
  • Jean Dubuisson
  • Takaji Wakita
  • Boyan Grigorov
  • David Durantel
  • Alessia Zamborlini
  • Birke Bartosch
  • Fabien Zoulim
  • Delphine Muriaux
  • Christian Trepo
  • Christophe Hourioux
  • Romuald Patient
  • Emmanuelle Blanchard
  • Rustem Uzbekov
  • Marion Depla
  • Eric Piver
  • Cynthia Chapel
  • Malika Ait-Goughoulte
  • Sylvie Trassard
  • Jean Christophe Pages
  • Alain Moreau
  • Julien Burlaud-Gaillard
  • Amélie Le Gouge
  • Pauline Ferraris
  • Emmanuelle Roch
  • Antoine Corbin
  • C Hourioux
  • Marie Lambelé
  • Jacqueline Lehmann-Che
  • Raymond A Dwek
  • Nicole Zitzmann
  • Céline Garcia
  • Yves Rouille
  • Pierre Yves Sizaret
  • Fabien Dorange
  • Sonia Georgeault
  • Jean Marc Guillaume
  • Claude Lebos
  • Caroline Sellin
  • Louis d'Alteroche
  • Yannick Bacq
  • Aurélie Avargues
  • Farhad Kazemi
  • Julien Gaillard
  • Anne De Muret
  • Bruno Giraudeau
  • Ludovic Gillet
  • Pascal Vaudin
  • Jean Luc Darlix
  • B Giraudeau
  • Emmanuel Clave
  • Ali Saib
  • R Patient
  • Noemie Renault
  • Francois Loic Cosset
  • Joelle Tobaly-Tapiero
  • Annette Martin
  • A Morin
  • Hugues De The
  • Patricia Bittoun
  • Marie Lou Giron
  • Fabrizio Mammano
  • E Blanchard
  • Fabienne Arcanger-Doudet
  • S Trassard
  • Antoine Toubert
  • A Moreau
  • Delphine Fouquenet
  • Geert Maertens
  • Cécile Voisset
  • David Delgrange
  • Arvind H Patel
  • François Helle
  • Czeslaw Wychowski
  • Jane McKeating
  • Sandrine Belouzard
  • Thierry Bru
  • Christine Collin
  • Frederic Dubois
  • Alain Goudeau

Detail Information

Publications35

  1. ncbi request reprint Hepatitis C virus ultrastructure and morphogenesis
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire et Laboratoire de Virologie, Faculté de Médecine ed Centre Hospitalier Universitaire, Tours, France
    Biol Cell 96:103-8. 2004
    ..The HCV-LP model opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions, which may make it possible to establish the long-awaited in vitro culture system for HCV...
  2. ncbi request reprint Correlative scanning-transmission electron microscopy reveals that a chimeric flavivirus is released as individual particles in secretory vesicles
    Julien Burlaud-Gaillard
    Plate Forme RIO des Microscopies, PPF ASB, Université François Rabelais and CHRU de Tours, Tours, France
    PLoS ONE 9:e93573. 2014
    ....
  3. pmc Viral sequence variation in chronic carriers of hepatitis C virus has a low impact on liver steatosis
    Marion Depla
    INSERM U966, Université François Rabelais and CHRU de Tours, Tours, France
    PLoS ONE 7:e33749. 2012
    ..Thus, individual host factors seem to play a much greater role than viral factors in the development of severe steatosis in patients chronically infected with HCV, including those infected with genotype 3 viruses...
  4. pmc Nuclear lipid droplets identified by electron microscopy of serial sections
    Rustem Uzbekov
    Plateforme des Microscopies, Université François Rabelais and CHRU de Tours, Tours, France
    BMC Res Notes 6:386. 2013
    ..The resolution of fluorescence confocal microscopy is not sufficiently high to exclude this hypothesis...
  5. doi request reprint [The lipid droplet: a new organelle?]
    Philippe Roingeard
    INSERM U966, Laboratoire de Biologie Cellulaire, université François Rabelais et CHU de Tours, 10, boulevard Tonnelle, 37032 Tours, France
    Med Sci (Paris) 29:495-500. 2013
    ..Nevertheless, they represent important cellular structures for which dysfunctions may lead to human diseases such as lypodystrophies or neurodegenerative diseases...
  6. doi request reprint Hepatitis C virus diversity and hepatic steatosis
    P Roingeard
    INSERM U966, Université François Rabelais and CHRU de Tours, Tours, France
    J Viral Hepat 20:77-84. 2013
    ..It is therefore difficult to distinguish between viral and metabolic steatosis unambiguously, and host and viral factors are probably involved in both HCV genotype 3 and nongenotype 3 steatosis...
  7. pmc Centrosomal pre-integration latency of HIV-1 in quiescent cells
    Alessia Zamborlini
    CNRS UMR7151, Universite Paris 7, Hopital Saint Louis, Paris, France
    Retrovirology 4:63. 2007
    ..Upon cell activation, viral replication resumes leading to viral gene expression. Thus, HIV-1 can persist in quiescent cells as a stable, centrosome-associated, pre-integration intermediate...
  8. ncbi request reprint Hepatitis C virus core protein, lipid droplets and steatosis
    P Roingeard
    INSERM ERI 19, Université François Rabelais and CHRU de Tours, Tours, France
    J Viral Hepat 15:157-64. 2008
    ..The association of HCV core protein with lipid droplets seems to play a central role in HCV pathogenesis and morphogenesis, suggesting that virus-induced steatosis may be essential for the viral life cycle...
  9. doi request reprint Hepatitis C virus budding at lipid droplet-associated ER membrane visualized by 3D electron microscopy
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, INSERM ERI 19, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours Cedex, France
    Histochem Cell Biol 130:561-6. 2008
    ..This may have important implications for our understanding of the complex relationship between HCV and lipids and may make easier to dissect out the HCV life cycle...
  10. doi request reprint Viral detection by electron microscopy: past, present and future
    Philippe Roingeard
    INSERM ERI 19 and Electron Microscopy Facility, Université François Rabelais and CHRU de Tours, Tours, France
    Biol Cell 100:491-501. 2008
    ..Recent examples of different viral assembly models illustrate the value of TEM for improving our understanding of virus-cell interactions...
  11. doi request reprint The birth and life of lipid droplets: learning from the hepatitis C virus
    Philippe Roingeard
    Laboratoire de Biologie Cellulaire, INSERM U966, Faculte de Medecine, Université François Rabelais and CHRU de Tours, 10 boulevard Tonnelle, 37032 Tours, France
    Biol Cell 103:223-31. 2011
    ..Recent studies in this model have raised the hypothesis that the HCV core protein induces the redistribution of LDs through the regression and regeneration of these organelles in specific intracellular domains...
  12. ncbi request reprint Antigenic properties of recombinant envelope glycoproteins derived from T-cell-line-adapted isolates or primary human immunodeficiency virus isolates and their relationship to immunogenicity
    D Brand
    Unité de virologie, Universite Francois Rabelais, Tours, France
    Virology 271:350-62. 2000
    ..Thus, it may be difficult to design an effective vaccine based on native recombinant PI envelopes...
  13. pmc The genotype 3-specific hepatitis C virus core protein residue phenylalanine 164 increases steatosis in an in vitro cellular model
    C Hourioux
    Universite Francois Rabelais, INSERM ERI 19 and CHRU de Tours, 10 boulevard Tonnelle, F 37032 Tours cedex, France
    Gut 56:1302-8. 2007
    ..An in vitro cellular model was used to investigate whether an HCV core protein with residues specific to genotype 3 increased this phenomenon...
  14. pmc Hepatitis C virus-like particle morphogenesis
    Emmanuelle Blanchard
    Laboratoire de Virologie, E3M EA3250, IFR 82, Faculte de Medecine et Centre Hospitalier Universitaire, Tours, France
    J Virol 76:4073-9. 2002
    ..This system opens up new possibilities for the investigation of viral morphogenesis and virus-host cell interactions...
  15. pmc Core protein cleavage by signal peptide peptidase is required for hepatitis C virus-like particle assembly
    Malika Ait-Goughoulte
    INSERM ESPRI 3856, IFR 136, Universite Francois Rabelais, Faculté de Médecine and CHU, 10 boulevard Tonnelle, 37032 Tours, France
    J Gen Virol 87:855-60. 2006
    ..These data shed light on the HCV core residues involved in SPP cleavage and suggest that this cleavage is essential for HCV assembly...
  16. doi request reprint The cell biology of hepatitis C virus (HCV) lipid addiction: molecular mechanisms and its potential importance in the clinic
    Eric Piver
    INSERM U966, Universite Francois Rabelais de Tours, Faculte de Medecine, 10 Bd Tonnellé, 37000 Tours, France piver
    Int J Biochem Cell Biol 42:869-79. 2010
    ..In the present review we summarise the current knowledge establishing the interplay between the molecular features of HCV replication, the cellular lipid biology and the lipid profiles observed in the serum of infected patients...
  17. pmc Impact of natural polymorphism within the gp41 cytoplasmic tail of human immunodeficiency virus type 1 on the intracellular distribution of envelope glycoproteins and viral assembly
    Marie Lambelé
    Universite Francois Rabelais, Tours, France
    J Virol 81:125-40. 2007
    ..Thus, polymorphisms in primary HIV-1 gp41 CTs at the quasispecies or subtype level can influence the intracellular distribution of Env, its incorporation into virions, and viral replication capacity...
  18. pmc Morphogenesis of hepatitis B virus and its subviral envelope particles
    Romuald Patient
    Universite Francois Rabelais, INSERM U 966, Tours, France
    Cell Microbiol 11:1561-70. 2009
    ..However, recent data clearly suggest that two different processes, including constitutive Golgi pathway or cellular machinery that generates internal vesicles of multivesicular bodies (MVB), independently form these two viral entities...
  19. doi request reprint Ultrastructural and quantitative analysis of the lipid droplet clustering induced by hepatitis C virus core protein
    Marion Depla
    INSERM U966, Faculte de Medecine, Universite Francois Rabelais, CHRU de Tours, 10 boulevard Tonnelle, 37032, Tours Cedex, France
    Cell Mol Life Sci 67:3151-61. 2010
    ..These observations provide useful information for further studies of the mechanisms involved in HCV-induced steatosis...
  20. doi request reprint Ultrastructural and biochemical analyses of hepatitis C virus-associated host cell membranes
    Pauline Ferraris
    INSERM U966, Université François Rabelais and CHRU de Tours, France
    J Gen Virol 91:2230-7. 2010
    ..More detailed characterization of these virus-cell interactions may facilitate the development of new treatments active against HCV and other RNA viruses that are dependent on newly synthesized cellular membranes for replication...
  21. pmc Hepatitis B virus subviral envelope particle morphogenesis and intracellular trafficking
    Romuald Patient
    Universite Francois Rabelais, INSERM ERI 19, Laboratoire de Biologie Cellulaire, Faculte de Medecine de Tours, 10 boulevard Tonnelle, F 37032 Tours cedex, France
    J Virol 81:3842-51. 2007
    ..These findings shed light on an important step in the HBV infectious cycle, as the intracellular accumulation of HBV subviral filaments may be directly linked to viral pathogenesis...
  22. doi request reprint Chimeric hepatitis B and C viruses envelope proteins can form subviral particles: implications for the design of new vaccine strategies
    Romuald Patient
    INSERM U966, Université François Rabelais and CHRU de Tours, France
    N Biotechnol 25:226-34. 2009
    ....
  23. ncbi request reprint Vesicular stomatitis virus glycoprotein: a transducing coat for SFV-based RNA vectors
    Fabien Dorange
    D3M, The Vector Group, Faculte de Medecine de Tours, 2 Bd Tonnelle, 37000 Tours, France
    J Gene Med 6:1014-22. 2004
    ..However, despite several modifications in the production process, a risk of contamination with replication-competent, or partially recombined, virus has remained...
  24. ncbi request reprint The transmembrane protein of HIV-1 primary isolates modulates cell surface expression of their envelope glycoproteins
    S Lebigot
    Unité de virologie, Département de Microbiologie Médicale et Moléculaire, EA3250 IFR 273, Universite Francois Rabelais, 37032 Tours, France
    Virology 290:136-42. 2001
    ..Identification of this signal may be important for the design of PI envelope-derived immunogens and may increase our understanding of the mechanisms by which HIV-1 escapes from the immune system...
  25. ncbi request reprint [Hepatitis C virus and lipid droplets]
    Philippe Roingeard
    Med Sci (Paris) 23:461-3. 2007
  26. pmc Endogenous virus and hepatitis C virus-like particle budding in BHK-21 cells
    Emmanuelle Blanchard
    J Virol 77:3888-9; author reply 3889. 2003
  27. pmc Hepatitis C virus-like particle budding: role of the core protein and importance of its Asp111
    Emmanuelle Blanchard
    Laboratoire de Virologie, Faculte de Medecine et Centre Hospitalier Universitaire, 2 bis Boulevard Tonnelle, 37032 Tours, France
    J Virol 77:10131-8. 2003
    ..In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis...
  28. ncbi request reprint Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines
    Boyan Grigorov
    LaboRetro Unité de virologie humaine INSERM U758, IFR128 Biosciences Lyon Gerland, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69 364 Lyon, France
    J Mol Biol 359:848-62. 2006
    ....
  29. ncbi request reprint Antiviral effect of alpha-glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particles
    Cynthia Chapel
    INSERM U271, Laboratoire des virus hépatiques et pathologies associées, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France
    J Gen Virol 87:861-71. 2006
    ..These alpha-glucosidase inhibitors may prove to be useful molecules to fight HCV infection in combination protocols...
  30. ncbi request reprint Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitors
    Cynthia Chapel
    INSERM, U871, Universite Lyon 1, et IFR62 Laennec, Lyon, France
    J Gen Virol 88:1133-43. 2007
    ..These properties suggest the potential usefulness of DNJ derivatives in combating HCV infection...
  31. pmc Centrosomal latency of incoming foamy viruses in resting cells
    Jacqueline Lehmann-Che
    Universite Paris 7, Paris, France
    PLoS Pathog 3:e74. 2007
    ..Incoming foamy retroviruses can stably persist at the centrosome, awaiting cell stimulation to initiate capsid cleavage, nuclear import, and viral gene expression...
  32. ncbi request reprint Vacuolization in hepatitis B virus-infected hepatocytes
    Philippe Roingeard
    Hepatology 37:1223-4; author reply 1224. 2003
  33. pmc Core protein domains involved in hepatitis C virus-like particle assembly and budding at the endoplasmic reticulum membrane
    Christophe Hourioux
    Universite Francois Rabelais de Tours, INSERM ERI 19 Tours, France
    Cell Microbiol 9:1014-27. 2007
    ....
  34. doi request reprint [Revisiting HIV-1 assembly]
    Antoine Corbin
    Laborétro Unité de virologie humaine, INSERM U758, IFR128, Ecole Normale Superieure de Lyon, 46 allee d Italie, Lyon, France
    Med Sci (Paris) 24:49-55. 2008
    ..Release of virions in the form of bursts would be triggered during cell-cell interactions, through a specialized structure called the virological synapse...
  35. pmc Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus
    Yves Rouille
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:2832-41. 2006
    ..In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle...