Daniel Rochu

Summary

Country: France

Publications

  1. Rochu D, Cléry Barraud C, Renault F, Chevalier A, Bon C, Masson P. Capillary electrophoresis versus differential scanning calorimetry for the analysis of free enzyme versus enzyme-ligand complexes: in the search of the ligand-free status of cholinesterases. Electrophoresis. 2006;27:442-51 pubmed
    ..Because inhibitors used in purification of ChEs by affinity chromatography are charged, CE remains a leading method to estimate enzyme stability and detect the presence of bound hidden ligands...
  2. Rochu D, Renault F, Cléry Barraud C, Chabriere E, Masson P. Stability of highly purified human paraoxonase (PON1): association with human phosphate binding protein (HPBP) is essential for preserving its active conformation(s). Biochim Biophys Acta. 2007;1774:874-83 pubmed
    ..A hybrid recombinant PON1 was shown more thermostable than the human enzyme, and its stability was unaffected by the presence of HPBP. Altogether, the results strongly encourage further study of the human enzyme. ..

Detail Information

Publications2

  1. Rochu D, Cléry Barraud C, Renault F, Chevalier A, Bon C, Masson P. Capillary electrophoresis versus differential scanning calorimetry for the analysis of free enzyme versus enzyme-ligand complexes: in the search of the ligand-free status of cholinesterases. Electrophoresis. 2006;27:442-51 pubmed
    ..Because inhibitors used in purification of ChEs by affinity chromatography are charged, CE remains a leading method to estimate enzyme stability and detect the presence of bound hidden ligands...
  2. Rochu D, Renault F, Cléry Barraud C, Chabriere E, Masson P. Stability of highly purified human paraoxonase (PON1): association with human phosphate binding protein (HPBP) is essential for preserving its active conformation(s). Biochim Biophys Acta. 2007;1774:874-83 pubmed
    ..A hybrid recombinant PON1 was shown more thermostable than the human enzyme, and its stability was unaffected by the presence of HPBP. Altogether, the results strongly encourage further study of the human enzyme. ..