Research Topics
Genomes and GenesSpecies
| Didier RaoultSummaryCountry: France Publications
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Detail Information
Publications
Molecular identification of a collection of spotted Fever group rickettsiae obtained from patients and ticks from RussiaStanislav N Shpynov
Unite des Rickettsies, Faculte de Medecine, Marseille, France
Am J Trop Med Hyg 74:440-3. 2006..Clinicians should be aware that several spotted fever rickettsioses with different prognoses coexist in Russia in areas where NATT was the only previously recognized rickettsiosis...
Tropheryma whipplei Twist: a human pathogenic Actinobacteria with a reduced genomeDidier Raoult
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Genome Res 13:1800-9. 2003..The analysis of the genome sequence of this previously largely unknown human pathogen is now guiding the development of molecular diagnostic tools and more convenient culture conditions...
Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticksPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 56:1673-5. 2006..Such genotypic and phenotypic characteristics warrant its classification as a representative of a novel species, for which the name Rickettsia tamurae sp. nov. is proposed, with the type strain AT-1(T) (=CSUR R1(T))...
Analysis of the Rickettsia africae genome reveals that virulence acquisition in Rickettsia species may be explained by genome reductionPierre Edouard Fournier
Unite des Rickettsies, IFR 48 CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, Marseille Cedex 05, France
BMC Genomics 10:166. 2009..prowazekii, which causes epidemic typhus and kills its arthropod host, to the mild pathogen R. africae, the agent of African tick-bite fever, which does not affect the fitness of its tick vector...
Genomic analysis of an emerging multiresistant Staphylococcus aureus strain rapidly spreading in cystic fibrosis patients revealed the presence of an antibiotic inducible bacteriophageJean Marc Rolain
URMITE CNRS IRD UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Biol Direct 4:1. 2009..We also conducted a retrospective epidemiological analysis on all S. aureus isolated from 2002 to 2007 in CF patients from our institution...
rpoB gene sequence-based characterization of emerging non-tuberculous mycobacteria with descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. novToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, 27, Boulevard Jean Moulin, Université de la Méditerranée and Assistance Publique Hôpitaux de Marseille Timone, Fédération de Microbiologie Clinique, Marseille, France
Int J Syst Evol Microbiol 56:133-43. 2006....
Molecular detection of Bartonella spp. in the dental pulp of stray cats buried for a yearGerard Aboudharam
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27, Boulevard Jean Moulin, Marseille Cedex 05 13385, France
Microb Pathog 38:47-51. 2005..grahamii (one cat). Our study indicates that dental pulp from the teeth of cats, especially the canine teeth, may be used for the PCR detection of Bartonella in animals buried for a year...
Rapid comparative genomic analysis for clinical microbiology: the Francisella tularensis paradigmBernard La Scola
Unite des Rickettsies CNRS UMR 6020, IFR 48, Faculte de Medecine de Marseille, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Genome Res 18:742-50. 2008..In the bioterrorism context, it allows the rapid detection of strain manipulation, including intentionally added virulence genes and genes that support antibiotic resistance...
Outbreak of Corynebacterium pseudodiphtheriticum infection in cystic fibrosis patients, FranceFadi Bittar
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 16:1231-6. 2010..Our study reemphasizes the conclusion that correctly identifying bacteria at the species level facilitates detection of an outbreak of new or emerging infections in humans...
Evidence for louse-transmitted diseases in soldiers of Napoleon's Grand Army in VilniusDidier Raoult
Unite des Rickettsies, Centre National de la Recherche Scientifique CNRS Unite Mixte de Recherche UMR 6020, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
J Infect Dis 193:112-20. 2006..Because soldiers were commonly infested with body lice, it has been speculated that louse-borne infectious diseases, such as epidemic typhus (caused by Rickettsia prowazekii), were common...
Rickettsia conorii transcriptional response within inoculation escharPatricia Renesto
Unite des Rickettsies, IRD CNRS UMR 6236, Faculte de Medecine, Marseille, France
PLoS ONE 3:e3681. 2008..The skin thus constitutes an important barrier for the entry and propagation of R. conorii. Given this, analysis of the survival strategies used by the bacterium within infected skin is critical for our understanding of rickettsiosis...
Nanobacteria are mineralo fetuin complexesDidier Raoult
Unite des Rickettsies, Centre National de la Recherche Scientifique UMR 6020, IFR 48, Faculte de Medecine, Marseille, France
PLoS Pathog 4:e41. 2008..quot; The presence of fetuin within renal calculi was also evidenced, suggesting its role as a hydroxyapatite nucleating factor...
Persistent Coxiella burnetii infection in mice overexpressing IL-10: an efficient model for chronic Q fever pathogenesisSoraya Meghari
Unite des Rickettsies, CNRS UMR 6020, Institut Federatif de Recherche 48, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
PLoS Pathog 4:e23. 2008..To our knowledge, this is the first efficient model for chronic Q fever pathogenesis...
Detection of a rickettsia closely related to Rickettsia aeschlimannii, "Rickettsia heilongjiangensis," Rickettsia sp. strain RpA4, and Ehrlichia muris in ticks collected in Russia and KazakhstanStanislav Shpynov
Unite des Rickettsies, IFR48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 5, France
J Clin Microbiol 42:2221-3. 2004..In Kazakhstan, we detected Rickettsia sp. strain RpA4 and a rickettsia closely related to Rickettsia aeschlimannii. These agents should be considered in a differential diagnosis of tick-borne infections in these areas...
A murine model of infection with Rickettsia prowazekii: implications for pathogenesis of epidemic typhusYassina Bechah
Unite des Rickettsies, CNRS UMR 6020, Universite de la Mediterranee, Faculte de Medecine, 27 Bld J Moulin, 13385 Marseille Cedex 05, France
Microbes Infect 9:898-906. 2007..We concluded that the BALB/c mouse strain provides a useful model for studying the pathogenic mechanisms of epidemic typhus and its control by the immune system...
Identification of two putative rickettsial adhesins by proteomic analysisPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
Res Microbiol 157:605-12. 2006..Both proteins are highly conserved within the Rickettsia genus and might play a critical role in their pathogenicity. These data may have important implications for the development of future vaccines against rickettsial infections...
MALDI-TOF-mass spectrometry applications in clinical microbiologyPiseth Seng
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille et URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Future Microbiol 5:1733-54. 2010..We will also discuss the application of MALDI-TOF-MS tools in the identification of Archaea, eukaryotes and viruses. Pathogenic identification from colony-cultured, blood-cultured, urine and environmental samples is also reviewed...
Value of Tropheryma whipplei quantitative polymerase chain reaction assay for the diagnosis of Whipple disease: usefulness of saliva and stool specimens for first-line screeningFlorence Fenollar
Unite des Rickettsies, Universite de la Mediterranee, Centre National de Recherche Scientifique, Marseille, France
Clin Infect Dis 47:659-67. 2008..Whipple disease (WD) is a chronic infectious disease caused by Tropheryma whipplei. WD DNA has been found in stool and saliva specimens from patients and asymptomatic carriers...
The transcriptional programme of human heart valves reveals the natural history of infective endocarditisMarie Benoit
Unité de Recherche sur les Maladies Infectieuses Transmissibles et Emergentes, Faculte de Medecine, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6236, Universite de la Mediterranee, Marseille, France
PLoS ONE 5:e8939. 2010..Some parameters identified in this work could become tools for measuring the disease activity and should be tested as biomarkers for diagnosis or prognosis assessment in future studies...
Deleterious effect of ciprofloxacin on Rickettsia conorii-infected cells is linked to toxin-antitoxin module up-regulationElisabeth Botelho-Nevers
URMITE UMR 6236, CNRS IRD, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
J Antimicrob Chemother 67:1677-82. 2012..To confirm and better understand the deleterious effect of fluoroquinolones reported during Rickettsia conorii infection in humans...
Tropheryma whipplei: a common bacterium in rural SenegalAlpha Kabinet Keita
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE CNRS IRD 198 UMR 6236, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
PLoS Negl Trop Dis 5:e1403. 2011....
Isolation of a rickettsia related to Astrakhan fever rickettsia from a patient in ChadPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseilles Cedex 05, France
Ann N Y Acad Sci 990:152-7. 2003..Although Astrakhan fever rickettsia is transmitted by Rhipicephalus ticks in Europe, further studies are indicated to identify its vector in Africa where these ticks are also prevalent...
Intracellular life of Coxiella burnetii in macrophagesEric Ghigo
URMITE CNRS UMR 6236 IRD 3R198, Institut Federatif de Recherche 48, Universite de la Mediterranee, Marseille, France
Ann N Y Acad Sci 1166:55-66. 2009..burnetii also depends on the functional state of myeloid cells. This review describes the mechanisms used by C. burnetii to circumvent uptake and trafficking events, and the role of cytokines on C. burnetii survival in myeloid cells...
Looking for Tropheryma whipplei source and reservoir in rural SenegalAlpha Kabinet Keita
Aix Marseille Universite, Unite des Rickettsies, Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, 13005 Marseille, France
Am J Trop Med Hyg 88:339-43. 2013..Humans are currently the predominant identified reservoir and source of T. whipplei in these populations. Limited access to toilets and exposure to human feces facilitate the fecal-oral transmission of T. whipplei...
Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometryBernard La Scola
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE UMR CNRS IRD 6236, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Intervirology 53:344-53. 2010..We attempted herein to isolate new Acanthamoeba-growing giant viruses from environmental samples...
The 1.2-megabase genome sequence of MimivirusDidier Raoult
Unite des Rickettsies, Faculte de Medecine, CNRS UMR6020, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
Science 306:1344-50. 2004..This new sequence data might help shed a new light on the origin of DNA viruses and their role in the early evolution of eukaryotes...
Yersinia massiliensis sp. nov., isolated from fresh waterVicky Merhej
AP HM Timone, Fédération de Microbiologie Clinique and Unité des Rickettsies CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Int J Syst Evol Microbiol 58:779-84. 2008..The name Yersinia massiliensis sp. nov. is proposed for this new taxon (type strain 50640T=CIP 109351T=CCUG 53443T; isolate 823=CIP 109352=CCUG 53444)...
Development of a method for recovering rickettsial RNA from infected cells to analyze gene expression profiling of obligate intracellular bacteriaMy Van La
Unite des Rickettsies, CNRS UMR6020, IFR48, Faculte de Medecine, 27, Bd Jean Moulin, Marseille, France
J Microbiol Methods 71:292-7. 2007..We conclude that this approach has great potential for the study of mechanisms behind the virulence and intracellular survival of members of the genus Rickettsia...
Heterogeneity of susceptibility to fluoroquinolones in Bartonella isolates from Australia reveals a natural mutation in gyrAEmmanouil Angelakis
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine et de Pharmacie Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
J Antimicrob Chemother 61:1252-5. 2008..Our objective was to evaluate in vitro antibiotic susceptibilities of 20 new Bartonella isolates from animals in Australia...
Multispacer typing to study the genotypic distribution of Bartonella henselae populationsWenjun Li
, IFR 48, CNRS UMR 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 44:2499-506. 2006..Finally, we provide a free-access MST-Rick online software program (http://ifr48.timone.univ-mrs.fr/MST_BHenselae/mst) that investigators may use to compare their own MST sequences to our database...
Actinomyces massiliensis sp. nov., isolated from a patient blood cultureAurelie Renvoise
Laboratoire de Bacteriologie virologie, Hopital de la Timone, CNRS UMR 6236, CNRS IRD, IFR48, 264 rue Saint Pierre, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 59:540-4. 2009..On the basis of phenotypic data and phylogenetic inference, the novel species Actinomyces massiliensis sp. nov. is proposed; the type strain is 4401292(T) (=CSUR P18(T)=CCUG 53522(T))...
Investigation of blood culture-negative early prosthetic valve endocarditis reveals high prevalence of fungiFranck Thuny
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS IRD UMR 6236, Faculte de Medecine, Universite de la Mediterranee, 27 Blvd Jean Moulin, 13385 Marseille Cedex 05, France
Heart 96:743-7. 2010..Therefore, fungi should be investigated particularly by molecular methods on surgical specimens and an antifungal drug might be added to the empiric treatment...
The uptake of apoptotic cells drives Coxiella burnetii replication and macrophage polarization: a model for Q fever endocarditisMarie Benoit
Unité de Recherche sur les Maladies Infectieuses Transmissibles et Emergentes, CNRS UMR 6236, Institut Federatif de Recherche 48, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
PLoS Pathog 4:e1000066. 2008..burnetii. We suggest that leukocyte apoptosis associated with valvulopathy may be critical for the pathogenesis of Q fever endocarditis by deactivating immune cells and creating a favorable environment for bacterial persistence...
Gene repertoire of amoeba-associated giant virusesPhilippe Colson
URMITE UMR CNRS 6236 IRD 198, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
Intervirology 53:330-43. 2010..Altogether, the data so far accumulated on amoeba-associated giant viruses are a powerful incentive to isolate and study additional strains to gain better understanding of their pangenome...
A novel alpha-Proteobacterium, Nordella oligomobilis gen. nov., sp. nov., isolated by using amoebal co-culturesBernard La Scola
Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterrannee, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Res Microbiol 155:47-51. 2004..nov., sp. nov. The genus Nordella forms a well separated taxon in the order Rhizobiales within the alpha-2 subgroup of Proteobacteria. Its close relatives are environmental uncultured bacteria...
Detection of Acinetobacter baumannii in human head and body lice from Ethiopia and identification of new genotypesMarie Kempf
URMITE, UMR IRD 198 CNRS 6236, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Int J Infect Dis 16:e680-3. 2012..The objectives of this study were to determine the presence of this bacterium in head and body lice collected from healthy individuals in Ethiopia by molecular methods and to characterize the genotype...
Description of Mycobacterium conceptionense sp. nov., a Mycobacterium fortuitum group organism isolated from a posttraumatic osteitis inflammationToidi Adekambi
Unite des Rickettsies, Faculte de Medecine, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 44:1268-73. 2006..porcinum. We propose the name Mycobacterium conceptionense sp. nov. for this new species associated with posttraumatic osteitis. The type strain is D16(T) (equivalent to CIP 108544(T) and CCUG 50187(T))...
Bartonella and Rickettsia in arthropods from the Lao PDR and from Borneo, MalaysiaTahar Kernif
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes URMITE, Universite de la Mediterranee, Marseille, France
Comp Immunol Microbiol Infect Dis 35:51-7. 2012..felis and B. clarridgeiae. Further investigations are needed in order to isolate these agents and to determine their epidemiology and aetiological role in unknown fever in patients from these areas...
Paenibacillus provencensis sp. nov., isolated from human cerebrospinal fluid, and Paenibacillus urinalis sp. nov., isolated from human urineVeronique Roux
Laboratoire de Bacteriologie virologie, Hopital de la Timone, CNRS UMR 6020, IFR48, 264 rue Saint Pierre, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 58:682-7. 2008..nov. (type strain 5402403(T) =CIP 109357(T) =CCUG 53521(T)) and Paenibacillus provencensis sp. nov. (type strain 4401170(T) =CIP 109358(T) =CCUG 53519(T))...
Tropheryma whipplei in children with gastroenteritisDidier Raoult
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 16:776-82. 2010..002). High incidence in patients and evidence of clonal circulation suggests that some cases of gastroenteritis are caused or exacerbated by T. whipplei, which may be co-transmitted with other intestinal pathogens...
The effect of a single dose of oral ivermectin on pruritus in the homelessSekene Badiaga
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 13185 Marseille, France
J Antimicrob Chemother 62:404-9. 2008..Homeless people commonly present with ectoparasite-based pruritus. We evaluated the efficacy of a single dose of ivermectin to reduce the pruritus prevalence in a homeless population...
Lipopolysaccharide from Coxiella burnetii is involved in bacterial phagocytosis, filamentous actin reorganization, and inflammatory responses through Toll-like receptor 4Amélie Honstettre
Unite des Rickettsies, Centre National de la Recherche Scientifique Unité Mixte de Recherche 6020, Institut Federatif de Recherche 48 Université de la Méditerranée, Marseille, France
J Immunol 172:3695-703. 2004..Taken together, these results demonstrate that TLR4 controls early events of C. burnetii infection such as macrophage phagocytosis, granuloma formation, and cytokine production...
Altitude-dependent Bartonella quintana genotype C in head lice, EthiopiaEmmanouil Angelakis
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 17:2357-9. 2011..B. quintana was found in 19 (7%) genotype C head lice and in 76 (18%) genotype A body lice. B. quintana in head lice was positively linked to altitude (p = 0.014)...
Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia speciesMaxime Ngwamidiba
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
BMC Microbiol 6:12. 2006....
Tropheryma whipplei glycosylation in the pathophysiologic profile of Whipple's diseaseCyrille J Bonhomme
Unite des Rickettsies, Centre National de la Recherche Scientifique 6236, Universite de la Mediterranee, Marseille, France
J Infect Dis 199:1043-52. 2009..Tropheryma whipplei is a bacterium commonly found in people with Whipple's disease, a rare systemic chronic infection. In the present study, we hypothesized that bacterium glycosylation may impair the immune response...
The virophage as a unique parasite of the giant mimivirusBernard La Scola
URMITE, Centre National de la Recherche Scientifique UMR IRD 6236, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France
Nature 455:100-4. 2008..Considering its functional analogy with bacteriophages, we classify this virus as a virophage. The virophage could be a vehicle mediating lateral gene transfer between giant viruses...
False positive PCR detection of Tropheryma whipplei in the saliva of healthy peopleJean Marc Rolain
Universite de la Mediterranee, Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Marseille Cedex 05, France
BMC Microbiol 7:48. 2007..whipplei in a series of 57 saliva from healthy people...
Prevalence of asymptomatic Tropheryma whipplei carriage among humans and nonhuman primatesFlorence Fenollar
Universite de la Mediterranee, Unite des Rickettsies, CNRS UMR 6020, Faculte de Medecine, Marseille Cedex, France
J Infect Dis 197:880-7. 2008..The reservoir of the agent of Whipple disease is unknown. Asymptomatic carriage of Tropheryma whipplei in human stool and saliva is controversial...
Coxiella burnetii infection in C57BL/6 mice aged 1 or 14 monthsMarc Leone
Unite des Rickettsies, Centre National de la Recherche Scientifique, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
FEMS Immunol Med Microbiol 50:396-400. 2007..In addition, macrophage microbicidal competence was similar in mature and young mice. These results suggest the importance of individual host factors in the pathophysiology of an infectious disease such as Q fever...
The giant Cafeteria roenbergensis virus that infects a widespread marine phagocytic protist is a new member of the fourth domain of LifePhilippe Colson
Centre National de la Recherche Scientifique Unité Mixte de Recherche 6236, Institut de Recherche pour le Développement 3R198, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
PLoS ONE 6:e18935. 2011..Recently, the genome of a new NCLDV, Cafeteria roenbergensis virus (CroV), was released. The present work aimed to determine if CroV supports the fourth domain of Life hypothesis...
Tropheryma whipplei bacteremia during fever in rural West AfricaFlorence Fenollar
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Universite de la Mediterranee, Faculte de Medecine, 1Marseille, France
Clin Infect Dis 51:515-21. 2010..We investigated the role of T. whipplei in febrile patients from rural Senegal who had a negative test result for malaria...
Bacterial strain typing in the genomic eraWenjun Li
Unité des Rickettsies et pathogènes émergents, CNRS UMR 6236, IRD 198, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
FEMS Microbiol Rev 33:892-916. 2009....
Legionella drancourtii sp. nov., a strictly intracellular amoebal pathogenBernard La Scola
, CNRS UPRESA 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 54:699-703. 2004..Taken together, these results support the proposal of LLAP12(T) as the type strain of Legionella drancourtii sp. nov...
Immuno-PCR--a new tool for paleomicrobiology: the plague paradigmNada Malou
Aix Marseille Universite, URMITE, UMR CNRS 6236 IRD 198, Faculte de Medecine, Marseille, France
PLoS ONE 7:e31744. 2012..However, the degradation of DNA limits the ability to detect ancient infections...
rpoB sequence-based identification of Mycobacterium avium complex speciesIskandar Ben Salah
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UMR, CNRS IRD 6236, IFR 48 Faculté de Médecine, Universite de la Mediterranee, Marseille, France
Microbiology 154:3715-23. 2008..Partial rpoB gene sequencing using the Myco-F/Myco-R primers permits one-step identification of MAC isolates at the species level and the detection of potentially novel MAC species...
Experimental model to evaluate the human body louse as a vector of plagueLinda Houhamdi
Unite des Rickettsies, Institut Federatif de Recherche 48, Centre National de Recherche Scientifique, Unite Mixte de Recherche 6020, Universite de la Mediterranee, Marseille Cedex 5 13385, France
J Infect Dis 194:1589-96. 2006..Infections were transmitted to naive lice that were fed on these rabbits, showing that lice can be vectors of Y. pestis in an experimental model...
Corpuscular antigenic microarray for the serodiagnosis of blood culture-negative endocarditisLaurent Samson
, CNRS UMR 6020, IFR 48, , , 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Ann N Y Acad Sci 1078:595-6. 2006..Preliminary results indicate that this new serologic test is valuable for the rapid, automated serological diagnosis of blood culture-negative endocarditis...
Ultrastructural characterization of the giant volcano-like virus factory of Acanthamoeba polyphaga MimivirusMarie Suzan-Monti
Unite des Rickettsies, Centre National de la Recherche Scientifique CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
PLoS ONE 2:e328. 2007....
Yersinia pestis Orientalis in remains of ancient plague patientsMichel Drancourt
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 13:332-3. 2007..We have now sequenced Y pestis glpD gene in such remains, showing a 93-bp deletion specific for biotype Orientalis. These data show that only Orientalis type caused the 3 plague pandemics...
Genotyping of human lice suggests multiple emergencies of body lice from local head louse populationsWenjun Li
URMITE, UMR CNRS 6236, IRD 198, Universite de la Mediterranee, Faculte de Medecine, Marseille, France
PLoS Negl Trop Dis 4:e641. 2010..Recent studies suggest that body lice arose several times from head louse populations...
Identification of rickettsial isolates at the species level using multi-spacer typingPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, Marseille Cedex 05, France
BMC Microbiol 7:72. 2007....
Genotyping reveals a wide heterogeneity of Tropheryma whippleiWenjun Li
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, Marseille Cedex 05, France
Microbiology 154:521-7. 2008..whipplei strains that is apparently independent of geographical distribution and unrelated to bacterial pathogenicity. Genotyping in Whipple's disease may, however, be useful in epidemiological studies...
Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and Methanogens in anorexic patientsFabrice Armougom
URMITE UMR CNRS 6236, IRD 3R198, Universite de la Mediterranee, Faculte de Medecine, and Nutrition and Diabetology Department, University Hospital Sainte Marguerite, Marseille, France
PLoS ONE 4:e7125. 2009..The roles of the archaeon Methanobrevibacter smithii and Lactobacillus species have not been described in these studies...
Molecular detection of multiple emerging pathogens in sputa from cystic fibrosis patientsFadi Bittar
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS IRD, UMR 6236, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
PLoS ONE 3:e2908. 2008..There is strong evidence that culture-based methods detect only a small proportion of bacteria present in the respiratory tracts of cystic fibrosis (CF) patients...
Validation of partial rpoB gene sequence analysis for the identification of clinically important and emerging Acinetobacter speciesVijay A K B Gundi
Unite des Rickettsies, CNRS UMR 6236, Faculte de Medecine de Marseille, 13385 Marseille Cedex 05, France
Microbiology 155:2333-41. 2009..Our data emphasize the recognition of A. schindleri as an emerging cause of Acinetobacter-related infection and confirm that A. gen. sp. 3 is the second most commonly isolated Acinetobacter species after A. baumannii in patients...
Emended description of Rickettsia felis (Bouyer et al. 2001), a temperature-dependent cultured bacteriumBernard La Scola
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille 05, France
Int J Syst Evol Microbiol 52:2035-41. 2002..felis and those observed for the isolate in this study indicated some differences, although concurrent analysis of the two was not possible as no extant isolates of the first isolate of R. felis exist...
Rickettsia conorii and R. prowazekii proteome analysis by 2DE-MS: a step toward functional analysis of rickettsial genomesPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, Marseille, France
Ann N Y Acad Sci 1063:90-3. 2005..This analysis reveals protein spots that were either unique to or common to both strains, some of them being identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry...
Analysis of 525 samples to determine the usefulness of PCR amplification and sequencing of the 16S rRNA gene for diagnosis of bone and joint infectionsFlorence Fenollar
, CNRS UMR 6020, IFR 48, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 44:1018-28. 2006..Seven organisms representing new potential species were also detected. PCR followed by cloning and sequencing may help to identify new pathogens involved in mixed bone infection...
Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometryPatricia Renesto
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille, France
FEMS Microbiol Lett 245:231-8. 2005..conorii, to compare protein profiles of different isolates or of bacteria maintained under different experimental conditions and to identify immunogenic proteins as potential vaccine targets...
Proposal to create subspecies of Rickettsia conorii based on multi-locus sequence typing and an emended description of Rickettsia conoriiYong Zhu
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
BMC Microbiol 5:11. 2005..Then, we selected a representative of each MLST genotype and used multi-spacer typing (MST) and mouse serotyping to estimate their degree of taxonomic relatedness...
Genetic identification of rickettsiae isolated from ticks in JapanPierre Edouard Fournier
Unite des Rickettsies, Faculte de Medecine, CNRS UMR 6020 27, Universite de la Mediterranee, 13385 Marseille, Cedex 05, France
J Clin Microbiol 40:2176-81. 2002..Finally, we identified a new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks...
Q FeverEmmanouil Angelakis
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Université de Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Vet Microbiol 140:297-309. 2010..Vaccination of animals in infected flocks, as well as in uninfected ones close to them, with an efficient vaccine can prevent abortions and shedding of the bacteria...
Blood culture-negative endocarditis in a reference center: etiologic diagnosis of 348 casesPierre Houpikian
, , , CNRS UPRES A 6020, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Medicine (Baltimore) 84:162-73. 2005..Further investigations should be focused on this group to identify new agents of infective endocarditis...
Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)Bernard La Scola
, CNRS UPRESA 6020, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 53:15-20. 2003..For treatment of infections caused by these novel bacteria, doxycycline appears to be the sole antibacterial compound with a consistently low MIC value...
Value of microimmunofluorescence for diagnosis and follow-up of Bartonella endocarditisPierre-Edouard Fournier
, IFR 48 CNRS, , , 13385 Marseille Cedex 05, France
Clin Diagn Lab Immunol 9:795-801. 2002..We propose that this criterion, which may also help with the detection of late relapses, be included as a major criterion in the Duke criteria for the diagnosis of infective endocarditis...
Bartonella quintana in a 4000-year-old human toothMichel Drancourt
Unite des Rickettsies, CNR UMR 6020, IFR 48, Marseille, France
J Infect Dis 191:607-11. 2005..Humans are the reservoir of B. quintana, the louse-borne agent of trench fever. We detected DNA of B. quintana in the dental pulp of a person who died 4000 years ago...
Bacillus massiliensis sp. nov., isolated from cerebrospinal fluidOlga O Glazunova
, , CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille Cedex 05, France
Int J Syst Evol Microbiol 56:1485-8. 2006..The major fatty acid was iso-C(15 : 0) (48 %). The name Bacillus massiliensis sp. nov. is proposed for the novel isolate, with strain 4400831(T) (=CIP 108446(T)=CCUG 49529(T)) as the type strain...
Gene sequence-based criteria for identification of new rickettsia isolates and description of Rickettsia heilongjiangensis sp. novPierre Edouard Fournier
Unite des Rickettsies, IFR 48, CNRS UMR 6020, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
J Clin Microbiol 41:5456-65. 2003..3% for the ompA and ompB genes and gene D, respectively. By use of our classification scheme, "Rickettsia heilongjiangii" belongs to a new species for which we officially propose the name Rickettsia heilongjiangensis sp. nov...
Regulation of whole bacterial pathogen transcription within infected hostsMy Van La
Unite des Rickettsies, Faculte de Medecine, 27 Boulevard Jean Moulin, Marseille, France
FEMS Microbiol Rev 32:440-60. 2008....
Usefulness of rpoB gene sequencing for identification of Afipia and Bosea species, including a strategy for choosing discriminative partial sequencesAtieh Khamis
Unite des Rickettsies, CNRS UPRESA 6020, Faculte de Medecine, Universite de la Mediterrannee, 13385 Marseille Cedex 05, France
Appl Environ Microbiol 69:6740-9. 2003....
"Candidatus Bartonella thailandensis": a new genotype of Bartonella identified from rodentsWatcharee Saisongkorh
Universite de la Mediterranee, Unite des Rickettsies, URMITE CNRS IRD, UMR 6236, Faculte de Medecine et de Pharmacie, Marseille 13385, France
Vet Microbiol 139:197-201. 2009..To the best of our knowledge, these Bartonella species have been detected for the first time in Thailand...
A bioinformatic approach to understanding antibiotic resistance in intracellular bacteria through whole genome analysisSilpak Biswas
URMITE UMR 6236, CNRS IRD, Faculte de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Int J Antimicrob Agents 32:207-20. 2008..In the future, stable mutants should be obtained through transformation methods in order to demonstrate experimentally the determinants of resistance in intracellular bacteria...
The rpoB gene as a tool for clinical microbiologistsToidi Adekambi
Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, CNRS IRD UMR 6236 IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Trends Microbiol 17:37-45. 2009..New identification tools targeting a rpoB gene fragment located between positions 2300 and 3300 have been developed recently. Therefore, inclusion of the rpoB gene sequence would be useful when describing new bacterial species...
Global transcriptome analysis of Tropheryma whipplei in response to temperature stressesNicolas Crapoulet
, CNRS UMR6020, IFR48, , 27, Boulevard Jean Moulin, 13385 Marseille, France
J Bacteriol 188:5228-39. 2006..whipplei exhibits an adaptive response to thermal stresses which is consistent with its specific environmental origin and could allow survival under cold conditions...
Amoebal coculture of "Mycobacterium massiliense" sp. nov. from the sputum of a patient with hemoptoic pneumoniaToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Université de la Méditteranée, Marseille, France
J Clin Microbiol 42:5493-501. 2004..The type strain has been deposited in the Collection Institut Pasteur as CIP 108297(T) and in Culture Collection of the University of Goteborg, Goteborg, Sweden, as CCUG 48898(T)...
Comparison between rpoB and 16S rRNA gene sequencing for molecular identification of 168 clinical isolates of CorynebacteriumAtieh Khamis
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterrannee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 43:1934-6. 2005..This method is thus a simple, molecular-analysis-based method for identification of corynebacteria, but it should be used in conjunction with other tests for definitive identification...
Correlation between ratio of serum doxycycline concentration to MIC and rapid decline of antibody levels during treatment of Q fever endocarditisJean-Marc Rolain
, CNRS UMR 6020, IFR 48, , , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
Antimicrob Agents Chemother 49:2673-6. 2005..burnetii cultured from this patient was resistant to doxycycline (MIC = 8 microg/ml). The ratio of serum doxycycline concentration to MIC should be monitored during the course of therapy in patients with Q fever endocarditis...
Genetic classification and differentiation of Bartonella species based on comparison of partial ftsZ gene sequencesZaher Zeaiter
, CNRS UMR 6020 IFR 48, , 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
J Clin Microbiol 40:3641-7. 2002..Our results show the usefulness of the portion of the ftsZ gene encoding the C-terminal region for diagnosis of Bartonella infections. More samples should be tested to study its usefulness for epidemiological investigations...
Phylogenetic classification of Bartonella species by comparing groEL sequencesZaher Zeaiter
Unite des Rickettsies, CNRS UPRES A 6020, Faculte de Medecine, Marseille, France
Int J Syst Evol Microbiol 52:165-71. 2002..In addition, the groEL gene was shown to be useful in subtyping six B. henselae isolates into three variants: Houston, Marseille and Fizz...
First detection of Wolbachia spp., including a new genotype, in sand flies collected in Marseille, FranceKotaro Matsumoto
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Marseille, France
J Med Entomol 45:466-9. 2008..These findings were confirmed by amplification, sequencing, and phylogenetic analysis of the wsp gene of the genus Wolbachia. The Wolbachia species from Sergentomyia minuta has been named "Wolbachia sp. detected in Se. minuta"...
Western-blot detection of RickA within spotted fever group rickettsiae using a specific monoclonal antibodyPremanand Balraj
Unite des Rickettsies, URMITE IRD CNRS 6236, Faculte de Medecine, Marseille, France
FEMS Microbiol Lett 286:257-62. 2008....
Cardiac valves in patients with Whipple endocarditis: microbiological, molecular, quantitative histologic, and immunohistochemical studies of 5 patientsHubert Lepidi
Unite des Rickettsies, Centre National de la Recherche Scientifique, Unite de Mixte de Recherche 6020, Institut Federatif Recherche 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
J Infect Dis 190:935-45. 2004..We report, for the first time, the immunodetection of T. whipplei in a surgically removed arterial embolus. Pathological and immunohistologic analyses may contribute to the diagnosis of Whipple endocarditis...
The expansion of the microbiological spectrum of brain abscesses with use of multiple 16S ribosomal DNA sequencingMouhamad Al Masalma
Pôle des Maladies Infectieuses, Assistance Publique Hôpitaux de Marseille and URMITE, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 6236, Institut pour la Recherche et le Développement 198, Universite de la Mediterranee, France
Clin Infect Dis 48:1169-78. 2009..Mycoplasma species are common and should be detected in this situation. These findings led us to question the accuracy of the current empirical treatment of brain abscess...
Cowpox virus transmission from pet rats to humans, FranceLaetitia Ninove
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 15:781-4. 2009..Pet rats, originating from the same pet store, were shown to be infected by a unique virus strain. Infection was then transmitted to humans who purchased or had contact with pet rats...
Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineationToidi Adekambi
Unite des Rickettsies, CNRS UMR 6020 IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
Int J Syst Evol Microbiol 58:1807-14. 2008..rpoB gene sequence similarity provides an efficient supplement to DDH and ANI measurements to delineate bacterial species and genera, including delineation of as-yet-uncultivated, non-sequenced organisms...
Endocarditis after acute Q fever in patients with previously undiagnosed valvulopathiesFlorence Fenollar
Unite des Rickettsies, Universite de la Mediterranee, Marseille, France
Clin Infect Dis 42:818-21. 2006..We conclude that, to prevent endocarditis, these minor valvulopathies must be actively searched for with echocardiography after diagnosis of acute Q fever...
Prevalence of Bartonella clarridgeiae and Bartonella henselae in domestic cats from France and detection of the organisms in erythrocytes by immunofluorescenceJean-Marc Rolain
, IFR 48, , , 13385 Marseille Cedex 05, France
Clin Diagn Lab Immunol 11:423-5. 2004..1% (8 of 99 cats). The intraerythrocytic location of Bartonella clarridgeiae is shown for the first time, and we show that immunofluorescence detection of the organism in erythrocytes correlates with the number of bacteria in blood...
Palaeomicrobiology: current issues and perspectivesMichel Drancourt
Unite des Rickettsies, CNRS UMR 6020, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
Nat Rev Microbiol 3:23-35. 2005....
Specific recognition of the major capsid protein of Acanthamoeba polyphaga mimivirus by sera of patients infected by Francisella tularensisNicolas Pelletier
URMITE, CNRS UMR IRD 6236, IFR 48, Faculte de Medecine, Marseille, France
FEMS Microbiol Lett 297:117-23. 2009..Furthermore, this cross-reactivity was confirmed with the recombinant capsid protein expressed in Escherichia coli. This emphasizes the pitfalls of a serological diagnosis of pneumonia...
Genetic diversity of Bartonella henselae in human infection detected with multispacer typingWenjun Li
Universite de la Mediterranee, Marseille, France
Emerg Infect Dis 13:1178-83. 2007..However, the diversity is not significantly different from that of cat strains. MST is thus a suitable genotyping tool for evaluating the genetic heterogeneity of B. henselae among isolates obtained from human patients...
Natural history of Bartonella infections (an exception to Koch's postulate)V Jacomo
, CNRS, UPRESA 6020, , , 27 Blvd. Jean Moulin, 13385 Marseille Cedex 05, France
Clin Diagn Lab Immunol 9:8-18. 2002
