Thierry Rabilloud

Summary

Country: France

Publications

  1. ncbi request reprint Oxidative stress response: a proteomic view
    Thierry Rabilloud
    DRDC ICH, INSERM U 548, CEA Laboratoire d Immunochimie, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Expert Rev Proteomics 2:949-56. 2005
  2. ncbi request reprint Keynotes on membrane proteomics
    Thierry Rabilloud
    INSERM U548, France
    Subcell Biochem 43:3-11. 2007
  3. ncbi request reprint Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
    Thierry Rabilloud
    Laboratoire d Immunologie, CEA, Grenoble, France
    Methods Mol Biol 355:111-9. 2007
  4. ncbi request reprint Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach
    Sarah Triboulet
    Univ Grenoble Alpes, Laboratory of Chemistry and Biology of Metals, UMR CNRS CEA UJF 5249, iRTSV LCBM, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Nanoscale 6:6102-14. 2014
  5. doi request reprint The whereabouts of 2D gels in quantitative proteomics
    Thierry Rabilloud
    CEA DSV iRTSV CBM and UMR CNRS UJF 5249, CEA Grenoble, Grenoble, France
    Methods Mol Biol 893:25-35. 2012
  6. doi request reprint Silver staining of 2D electrophoresis gels
    Thierry Rabilloud
    CEA DSV iRTSV CBM and UMR CNRS UJF 5249, CEA Grenoble, Grenoble, France
    Methods Mol Biol 893:61-73. 2012
  7. doi request reprint Two-dimensional gel electrophoresis in proteomics: Past, present and future
    Thierry Rabilloud
    CNRS UMR5092, Biochemistry and Biophysics of Integrated Systems, CEA Grenoble, iRTSV BSBBSI, Grenoble, France
    J Proteomics 73:2064-77. 2010
  8. doi request reprint Membrane proteins and proteomics: love is possible, but so difficult
    Thierry Rabilloud
    CEA DSV iRTSV LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA Grenoble, Grenoble, France
    Electrophoresis 30:S174-80. 2009
  9. doi request reprint Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update
    Thierry Rabilloud
    CEA DSV iRTSV LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA Grenoble, Grenoble, France
    Proteomics 8:3965-73. 2008
  10. doi request reprint Two-dimensional gel electrophoresis in proteomics: a tutorial
    Thierry Rabilloud
    CNRS UMR5249, Chemistry and Biology of Metals, CEA Grenoble, iRTSV BSBBSI, Grenoble Cedex 9, France
    J Proteomics 74:1829-41. 2011

Collaborators

Detail Information

Publications50

  1. ncbi request reprint Oxidative stress response: a proteomic view
    Thierry Rabilloud
    DRDC ICH, INSERM U 548, CEA Laboratoire d Immunochimie, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Expert Rev Proteomics 2:949-56. 2005
    ..The input of proteomics in the study of oxidative stress response and in the evidence of an oxidative stress component in biologic phenomena is reviewed in this paper...
  2. ncbi request reprint Keynotes on membrane proteomics
    Thierry Rabilloud
    INSERM U548, France
    Subcell Biochem 43:3-11. 2007
  3. ncbi request reprint Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
    Thierry Rabilloud
    Laboratoire d Immunologie, CEA, Grenoble, France
    Methods Mol Biol 355:111-9. 2007
    ..These solutions deal mainly with chaotropes and new detergents, which are both able to enhance protein solubility. The input of these compounds in proteomics analysis of membrane proteins is discussed, as well as future directions...
  4. ncbi request reprint Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach
    Sarah Triboulet
    Univ Grenoble Alpes, Laboratory of Chemistry and Biology of Metals, UMR CNRS CEA UJF 5249, iRTSV LCBM, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Nanoscale 6:6102-14. 2014
    ..Conversely, glutathione levels and phagocytosis appear unaffected at moderately toxic zinc concentrations. ..
  5. doi request reprint The whereabouts of 2D gels in quantitative proteomics
    Thierry Rabilloud
    CEA DSV iRTSV CBM and UMR CNRS UJF 5249, CEA Grenoble, Grenoble, France
    Methods Mol Biol 893:25-35. 2012
    ....
  6. doi request reprint Silver staining of 2D electrophoresis gels
    Thierry Rabilloud
    CEA DSV iRTSV CBM and UMR CNRS UJF 5249, CEA Grenoble, Grenoble, France
    Methods Mol Biol 893:61-73. 2012
    ..Both features are discussed in this chapter, and optimized silver staining protocols are proposed...
  7. doi request reprint Two-dimensional gel electrophoresis in proteomics: Past, present and future
    Thierry Rabilloud
    CNRS UMR5092, Biochemistry and Biophysics of Integrated Systems, CEA Grenoble, iRTSV BSBBSI, Grenoble, France
    J Proteomics 73:2064-77. 2010
    ..Taking support of these advantages (robustness, resolution, and ability to separate entire, intact proteins), possible future applications of this technique in proteomics are also mentioned...
  8. doi request reprint Membrane proteins and proteomics: love is possible, but so difficult
    Thierry Rabilloud
    CEA DSV iRTSV LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA Grenoble, Grenoble, France
    Electrophoresis 30:S174-80. 2009
    ..Last but not least, this review also critically examines the alternate approaches that have been used for the proteomic analysis of membrane proteins...
  9. doi request reprint Fully denaturing two-dimensional electrophoresis of membrane proteins: a critical update
    Thierry Rabilloud
    CEA DSV iRTSV LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA Grenoble, Grenoble, France
    Proteomics 8:3965-73. 2008
    ..e., electrophoresis in the presence of cationic or anionic detergents. The overall performances and fields of application of both types of method is critically examined, as are future prospects for this field...
  10. doi request reprint Two-dimensional gel electrophoresis in proteomics: a tutorial
    Thierry Rabilloud
    CNRS UMR5249, Chemistry and Biology of Metals, CEA Grenoble, iRTSV BSBBSI, Grenoble Cedex 9, France
    J Proteomics 74:1829-41. 2011
    ..This is illustrated by a detailed example taken from the literature and commented in detail. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP 2)...
  11. doi request reprint Power and limitations of electrophoretic separations in proteomics strategies
    Thierry Rabilloud
    CEA, iRTSV, LBBSI, 38054 Grenoble, France
    Mass Spectrom Rev 28:816-43. 2009
    ..e., protein detection after electrophoresis, and the process leading from a gel piece to a solution of peptides) are also discussed...
  12. doi request reprint Variations on a theme: changes to electrophoretic separations that can make a difference
    Thierry Rabilloud
    CNRS UMR5092, Biochemistry and Biophysics of Integrated Systems, CEA Grenoble, iRTSV BSBBSI, Grenoble, France
    J Proteomics 73:1562-72. 2010
    ....
  13. doi request reprint Is a gene-centric human proteome project the best way for proteomics to serve biology?
    Thierry Rabilloud
    Biochemistry and Biophysics of Integrated Systems, UMR CNRS CEA UJF 5092, CEA Grenoble, iRTSV BSBBSI, Grenoble, France
    Proteomics 10:3067-72. 2010
    ..In this paper, we try to shed light on what these definitions mean, how large-scale proteomics such as a HPP can insert into the larger omics chorus, and what we can reasonably expect from a HPP in the way it has been proposed so far...
  14. pmc Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA: A proteomic view
    Mireille Chevallet
    CEA, Laboratoire d Immunochimie, DRDC ICH, INSERM U 548, CEA Grenoble, Grenoble, France
    Electrophoresis 27:1574-83. 2006
    ..Examples include a stomatin-like protein and a protein sharing homologies with bacterial proteins implicated in tyrosine catabolism. Proteins involved in apoptosis control are also found modulated in Rho-0 mitochondria...
  15. ncbi request reprint Regeneration of peroxiredoxins during recovery after oxidative stress: only some overoxidized peroxiredoxins can be reduced during recovery after oxidative stress
    Mireille Chevallet
    Commissariat à l Energie Atomique Laboratoire de Bioénergétique Cellulaire et Pathologique, EA 2943, Département Réponses et Dynamique Cellulaire BioEnergétique Cellulaire et Pathologique Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    J Biol Chem 278:37146-53. 2003
    ..This demonstrates that the overoxidation can be reversible under certain conditions. The pathway of this retroreduction and the reasons explaining the various regeneration speeds of the peroxiredoxins remain to be elucidated...
  16. doi request reprint Improved proteomic analysis of nuclear proteins, as exemplified by the comparison of two myeloid cell lines nuclear proteomes
    Cécile Lelong
    Laboratoire de Chimie et Biologie des Metaux, Universite Joseph Fourier Grenoble, iRTSV LCBM, CEA Grenoble, 17 Avenue des Martyrs, 38054 Grenoble Cedex 9, France
    J Proteomics 77:577-602. 2012
    ....
  17. pmc Improved mass spectrometry compatibility is afforded by ammoniacal silver staining
    Mireille Chevallet
    CEA Laboratoire d Immunochimie, DRDC ICH, INSERM U 548, CEA Grenoble, Grenoble, France
    Proteomics 6:2350-4. 2006
    ..This influences in turn the sequence coverage and thus the detail of the analysis provided by the mass spectrometer...
  18. pmc A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins
    Christophe Tastet
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, Grenoble, France
    Electrophoresis 24:1787-94. 2003
    ..Coupled with limited variation in the acrylamide concentration, this electrophoresis system allows to tailor the resolution in the 6-200 kDa M(r) range, with minimal difficulties in the post electrophoretic identification processes...
  19. ncbi request reprint Progress in the definition of a reference human mitochondrial proteome
    Pierre Lescuyer
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, DBMS BECP, Grenoble, France
    Proteomics 3:157-67. 2003
    ....
  20. doi request reprint Preparing protein extracts for quantitative two-dimensional gel comparison
    Mireille Chevallet
    Départment Résponse et Dynamique, Cellulaire BioEnergetique Cellulaire et Pathologique, Commissariat a l Energie Atomique, Grenoble, France
    Curr Protoc Protein Sci . 2004
    ..These solubilization procedures take into account the constraints brought by two-dimensional electrophoresis and are thus well suited for proteomic approaches...
  21. ncbi request reprint Ultrafast coelectrophoretic fluorescent staining of proteins with carbocyanines
    Sylvie Luche
    CEA Laboratoire de Biochimie et Biophysique des systèmes Intégrés, iRTSV BBSI, CEA Grenoble, Grenoble, France
    Proteomics 7:3234-44. 2007
    ..Excellent sequence coverage in subsequent MS analysis of proteolytic peptides is also achieved with this detection method...
  22. ncbi request reprint Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis
    Sylvie Luche
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, EA 2943, DRDC BECP, CEA Grenoble, France
    Proteomics 3:249-53. 2003
    ..They complement the more classical sulfobetaine detergents to widen the scope of useful detergents for the solubilization of membrane proteins in proteomics...
  23. doi request reprint Sweet silver: a formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry
    Mireille Chevallet
    CEA Laboratoire de Biochimie et Biophysique des systèmes Intégrés, iRTSV BBSI, CEA Grenoble, Grenoble, France
    Proteomics 8:4853-61. 2008
    ..While keeping the benefits of silver staining, this method is shown to afford a much better performance in terms of compatibility with MS, both in PMF by MALDI and in LC/ESI/MS/MS...
  24. doi request reprint Zinc adaptation and resistance to cadmium toxicity in mammalian cells: molecular insight by proteomic analysis
    Estelle Rousselet
    CEA, DSV, iRTSV, Laboratoire de Chimie et Biologie des Metaux, Grenoble, France
    Proteomics 8:2244-55. 2008
    ..Inhibition of HPPD decreased the resistance of HZR cells against cadmium, but not that of HeLa cells, suggesting that adaptation to zinc overload and increased HPP removal are linked in HZR cells...
  25. doi request reprint Organelle proteomics
    Pierre Lescuyer
    CEA Grenoble, Grenoble, France
    Curr Protoc Protein Sci . 2006
    ..A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided...
  26. doi request reprint Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method
    Catherine Aude-Garcia
    CEA DSV iRTSV LBBSI, Biophysique et Biochimie des Systèmes Intégrés, CEA Grenoble, Grenoble, France
    Proteomics 11:324-8. 2011
    ..We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method...
  27. doi request reprint Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis
    Thierry Rabilloud
    Laboratoire, d immunologie, INSERM, Grenoble, France
    Methods Mol Biol 528:259-67. 2009
    ....
  28. doi request reprint Silver staining of proteins in 2DE gels
    Cécile Lelong
    CEA DSV iRTSV LBBSI and UMR CNRS 5092, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Methods Mol Biol 519:339-50. 2009
    ..Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 day after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks...
  29. doi request reprint Dual roles for MEF2A and MEF2D during human macrophage terminal differentiation and c-Jun expression
    Catherine Aude-Garcia
    Commissariat a l Energie Atomique, DSV, iRTSV, Laboratoire BBSI, Grenoble, France
    Biochem J 430:237-44. 2010
    ..Nevertheless, these data highlight for the first time the possible dual roles of MEF2A and MEF2D in human macrophages, as activators or as repressors of gene transcription...
  30. ncbi request reprint [Concepts and therapeutic perspectives of proteomics]
    Pierre Lescuyer
    Laboratoire de Bioénergétique Cellulaire et Pathologique, DRDC BECP, CEA Grenoble, 17, rue des Martyrs, 38054 Grenoble 9, France
    Med Sci (Paris) 20:587-92. 2004
    ..Their performances and limitations are outlined, as well as their potential applications in biomedicine, to give an reasoned view of the current state of the art...
  31. doi request reprint Mitochondrial proteomics : analysis of a whole mitochondrial extract with two-dimensional electrophoresis
    Thierry Rabilloud
    CEA DSV IRTSV BBSI, UMR CNRS 5092, Biologie et Biophysique des systèmes intégrés, CEA Grenoble, 17 rue des Martyrs, Grenoble, France
    Methods Mol Biol 432:83-100. 2008
    ..This covers both the protein solubilization, the electrophoretic part per se, and the protein detection on the gels, which makes the interface with the protein identification part relying on mass spectrometry...
  32. ncbi request reprint A survey of the plant mitochondrial proteome in relation to development
    Juliette Bardel
    Laboratoire de Physiologie Cellulaire Végétale, DRDC, CEA Grenoble, Grenoble, France
    Proteomics 2:880-98. 2002
    ....
  33. ncbi request reprint Proteomics analysis of cellular response to oxidative stress. Evidence for in vivo overoxidation of peroxiredoxins at their active site
    Thierry Rabilloud
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, EA UJF 2943, DRDC BECP, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    J Biol Chem 277:19396-401. 2002
    ....
  34. pmc Toward a better analysis of secreted proteins: the example of the myeloid cells secretome
    Mireille Chevallet
    CEA DSV DRDC ICH, Laboratoire d Immunochimie, CEA Grenoble, Grenoble, France
    Proteomics 7:1757-70. 2007
    ....
  35. pmc Silver staining of proteins in polyacrylamide gels
    Mireille Chevallet
    CEA Grenoble, DRDC ICH, 38054 Grenoble, Cedex 9, France
    Nat Protoc 1:1852-8. 2006
    ..Several variants of silver staining are described here, which can be completed in a time range from 2 h to 1 d after the end of the electrophoretic separation. Once completed, the stain is stable for several weeks...
  36. doi request reprint Two-dimensional SDS-PAGE fractionation of biological samples for biomarker discovery
    Thierry Rabilloud
    Chemistry and Biology of Metals Grenoble, CEA Grenoble, Grenoble, France
    Methods Mol Biol 1002:151-65. 2013
    ....
  37. doi request reprint Solubilization of proteins in 2DE: an outline
    Thierry Rabilloud
    CEA DSV iRTSV LBBSI and UMR CNRS 5092, CEA Grenoble, 17 rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Methods Mol Biol 519:19-30. 2009
    ..The constraints brought in this process by the peculiar features of isoelectric focusing are discussed, as well as their consequences in terms of possible solutions and limits for the solubilization process...
  38. ncbi request reprint How to use 2D gel electrophoresis in plant proteomics
    Thierry Rabilloud
    Chemistry and Biology of Metals, CNRS UMR 5249, Grenoble, France
    Methods Mol Biol 1072:43-50. 2014
    ..These features are detailed in this chapter, as is the rationale for selecting or not 2D gel-based proteomics as a proteomic strategy. ..
  39. ncbi request reprint Comparative proteomics as a new tool for exploring human mitochondrial tRNA disorders
    Thierry Rabilloud
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, UA 2019 DBMS BECP, 17, rue des Martyrs, F 38054 Grenoble Cedex 9, France
    Biochemistry 41:144-50. 2002
    ..It opens new routes toward a large-scale exploration of potential proteic partners involved in the genotype-phenotype correlation of mitochondrial disorders...
  40. ncbi request reprint Structure-efficiency relationships of zwitterionic detergents as protein solubilizers in two-dimensional electrophoresis
    Christophe Tastet
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, DRDC BECP, CEA Grenoble, Grenoble, France
    Proteomics 3:111-21. 2003
    ....
  41. doi request reprint The proteomic to biology inference, a frequently overlooked concern in the interpretation of proteomic data: a plea for functional validation
    Thierry Rabilloud
    CNRS UMR 5249, Laboratory of Chemistry and Biology of Metals, Grenoble, France Univ Grenoble Alpes, Laboratory of Chemistry and Biology of Metals, Grenoble, France CEA Grenoble, iRTSV CBM, Laboratory of Chemistry and Biology of Metals, Grenoble, France
    Proteomics 14:157-61. 2014
    ..e. not merely the confirmation of protein abundance changes by other methods, but a functional appraisal of the biological consequences of the protein level changes highlighted by the proteomic screens. ..
  42. ncbi request reprint Two-dimensional gel electrophoresis in proteomics: old, old fashioned, but it still climbs up the mountains
    Thierry Rabilloud
    CEA Laboratoire de Bioénergétique Cellulaire et Pathologique, Grenoble, France
    Proteomics 2:3-10. 2002
    ..The purpose of this paper is thus to highlight the strengths and weaknesses of all the proteomics approaches proposed to date and to try to deduce the respective niches in proteomics that these approaches will have in the future...
  43. pmc A method for detection of overoxidation of cysteines: peroxiredoxins are oxidized in vivo at the active-site cysteine during oxidative stress
    Elsa Wagner
    Laboratoire de Spectrometrie de Masse Bio Organique, UMR CNRS 7509, ECPM, 25 rue Becquerel, 67087 Strasbourg Cedex 2, France
    Biochem J 366:777-85. 2002
    ..Thus, this acidic variant represents an inactivation form of the peroxiredoxins, and provides a useful marker of oxidative damage to the cells...
  44. ncbi request reprint Membrane proteins ride shotgun
    Thierry Rabilloud
    Nat Biotechnol 21:508-10. 2003
  45. ncbi request reprint Proteomic analysis identifies novel proteins of the Maurer's clefts, a secretory compartment delivering Plasmodium falciparum proteins to the surface of its host cell
    Laetitia Vincensini
    Unité de Biologie des Interactions Hôte Parasite, CNRS URA 2581, Institut Pasteur, 25 28 Rue du Dr Roux, 75724 Paris Cedex 15, France
    Mol Cell Proteomics 4:582-93. 2005
    ..Our study sheds new light on the biological function of the Maurer's clefts, which are central to the pathogenesis and to the intraerythrocytic development of P. falciparum...
  46. ncbi request reprint Guidelines for the next 10 years of proteomics
    Marc R Wilkins
    Department of Biotechnology and Biomolecular Biosciences, University of New South Wales, Sydney, NSW 2052, Australia
    Proteomics 6:4-8. 2006
    ..These guidelines will serve as a set of criteria which editors of PROTEOMICS will use for assessment of future submissions to the Journal...
  47. ncbi request reprint Protein arginylation in rat brain cytosol: a proteomic analysis
    María Belén Decca
    Centro de Investigaciones en Quimica Biologica de Cordoba, CIQUIBIC, UNC CONICET, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, 5000, Cordoba, Argentina
    Neurochem Res 31:401-9. 2006
    ..Therefore, we postulate that the substrates for arginylation could reach the cytosol by retro-translocation and be then arginylated...
  48. ncbi request reprint How shall we use the proteomics toolbox for biomarker discovery?
    Pierre Lescuyer
    Department of Structural Biology and Bioinformatics, Faculty of Medicine and Pharmacy Section, Faculty of Sciences, Geneva University, Geneva, Switzerland
    J Proteome Res 6:3371-6. 2007
    ..Two major strategies are analyzed, namely, the switch from body fluids to cell and tissues for the initial biomarker discovery step or, if body fluids must be analyzed, the implementation of highly selective protein selection strategies...
  49. ncbi request reprint The Crl-RpoS regulon of Escherichia coli
    Cécile Lelong
    Laboratoire Adaptation et Pathogenie des Microorganismes, Institut Jean Roget, Campus de la Merci, Universite Joseph Fourier, BP170, 38042 Grenoble Cedex 9, France
    Mol Cell Proteomics 6:648-59. 2007
    ..Crl may thus be considered a new environmental sensor via the indole concentration...
  50. doi request reprint Guidelines for reporting the use of gel electrophoresis in proteomics
    Frank Gibson
    Nat Biotechnol 26:863-4. 2008