Alain Pruvost

Summary

Country: France

Publications

  1. ncbi Pilot pharmacokinetic study of human immunodeficiency virus-infected patients receiving tenofovir disoproxil fumarate (TDF): investigation of systemic and intracellular interactions between TDF and abacavir, lamivudine, or lopinavir-ritonavir
    Alain Pruvost
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, PC no 18, F 91191 Gif sur Yvette, France
    Antimicrob Agents Chemother 53:1937-43. 2009
  2. ncbi Sensitive and specific LC-ESI-MS/MS method for the determination of a styrylquinoline, BA011FZ041, a potent HIV anti-integrase agent, in rat plasma
    Alain Pruvost
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Chromatogr B Analyt Technol Biomed Life Sci 850:259-66. 2007
  3. ncbi Specificity enhancement with LC-positive ESI-MS/MS for the measurement of nucleotides: application to the quantitative determination of carbovir triphosphate, lamivudine triphosphate and tenofovir diphosphate in human peripheral blood mononuclear cells
    Alain Pruvost
    CEA, iBiTecS, SPI, Laboratoire d Etude du Métabolisme des Médicaments, Gif sur Yvette, F 91191, France
    J Mass Spectrom 43:224-33. 2008
  4. ncbi Measurement of intracellular didanosine and tenofovir phosphorylated metabolites and possible interaction of the two drugs in human immunodeficiency virus-infected patients
    Alain Pruvost
    CEA, Service de Pharmacologie et d'Immunologie, DSV/DRM, CEA/Saclay, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 49:1907-14. 2005
  5. ncbi Evidence and possible consequences of the phosphorylation of nucleoside reverse transcriptase inhibitors in human red blood cells
    Lucie Durand Gasselin
    Service de Pharmacologie et d immunoanalyse, Batiment 136, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 51:2105-11. 2007
  6. ncbi A strategy for liquid chromatography/tandem mass spectrometric assays of intracellular drugs: application to the validation of the triphosphorylated anabolite of antiretrovirals in peripheral blood mononuclear cells
    François Becher
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Mass Spectrom 38:879-90. 2003
  7. ncbi Development and validation of a liquid chromatographic/tandem mass spectrometric assay for the quantitation of nucleoside HIV reverse transcriptase inhibitors in biological matrices
    Séverine Compain
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Mass Spectrom 40:9-18. 2005
  8. ncbi Liquid chromatography-tandem mass spectrometry assays for intracellular deoxyribonucleotide triphosphate competitors of nucleoside antiretrovirals
    Gaëlle Henneré
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    J Chromatogr B Analyt Technol Biomed Life Sci 789:273-81. 2003
  9. ncbi High levels of zidovudine (AZT) and its intracellular phosphate metabolites in AZT- and AZT-lamivudine-treated newborns of human immunodeficiency virus-infected mothers
    Lucie Durand-Gasselin
    Service de Pharmacologie et d immunoanalyse, CEA, iBiTecS, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 52:2555-63. 2008
  10. ncbi Emtricitabine: Inhibitor and substrate of multidrug resistance associated protein
    Laurence Bousquet
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette Cedex, France
    Eur J Pharm Sci 35:247-56. 2008

Collaborators

Detail Information

Publications18

  1. ncbi Pilot pharmacokinetic study of human immunodeficiency virus-infected patients receiving tenofovir disoproxil fumarate (TDF): investigation of systemic and intracellular interactions between TDF and abacavir, lamivudine, or lopinavir-ritonavir
    Alain Pruvost
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, PC no 18, F 91191 Gif sur Yvette, France
    Antimicrob Agents Chemother 53:1937-43. 2009
    ..A trend for a gender effect was observed for TFV-DP at the intracellular level, with higher and C(trough) values for women...
  2. ncbi Sensitive and specific LC-ESI-MS/MS method for the determination of a styrylquinoline, BA011FZ041, a potent HIV anti-integrase agent, in rat plasma
    Alain Pruvost
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Chromatogr B Analyt Technol Biomed Life Sci 850:259-66. 2007
    ..Intra- and inter-assay precision and accuracy values were within the regulatory limits. The method was successfully applied to the determination of BA011FZ041 in rat plasma and PBMCs after i.v. dosing...
  3. ncbi Specificity enhancement with LC-positive ESI-MS/MS for the measurement of nucleotides: application to the quantitative determination of carbovir triphosphate, lamivudine triphosphate and tenofovir diphosphate in human peripheral blood mononuclear cells
    Alain Pruvost
    CEA, iBiTecS, SPI, Laboratoire d Etude du Métabolisme des Médicaments, Gif sur Yvette, F 91191, France
    J Mass Spectrom 43:224-33. 2008
    ..The use of positive ESI, offering a better specificity and a slightly better sensitivity than the negative ESI mode for these compounds, resulted in specificity enhancement and more robust assay methods...
  4. ncbi Measurement of intracellular didanosine and tenofovir phosphorylated metabolites and possible interaction of the two drugs in human immunodeficiency virus-infected patients
    Alain Pruvost
    CEA, Service de Pharmacologie et d'Immunologie, DSV/DRM, CEA/Saclay, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 49:1907-14. 2005
    ..5 days). This characteristic is certainly interesting in terms of efficacy but could have some drawbacks in terms of virus resistance for patients discontinuing these drugs...
  5. ncbi Evidence and possible consequences of the phosphorylation of nucleoside reverse transcriptase inhibitors in human red blood cells
    Lucie Durand Gasselin
    Service de Pharmacologie et d immunoanalyse, Batiment 136, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 51:2105-11. 2007
    ..Thus, we recommend removing RBC during PBMC preparation by using an ammonium chloride solution to enhance both the accuracy and the precision of intracellular drug monitoring...
  6. ncbi A strategy for liquid chromatography/tandem mass spectrometric assays of intracellular drugs: application to the validation of the triphosphorylated anabolite of antiretrovirals in peripheral blood mononuclear cells
    François Becher
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Mass Spectrom 38:879-90. 2003
    ....
  7. ncbi Development and validation of a liquid chromatographic/tandem mass spectrometric assay for the quantitation of nucleoside HIV reverse transcriptase inhibitors in biological matrices
    Séverine Compain
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    J Mass Spectrom 40:9-18. 2005
    ....
  8. ncbi Liquid chromatography-tandem mass spectrometry assays for intracellular deoxyribonucleotide triphosphate competitors of nucleoside antiretrovirals
    Gaëlle Henneré
    CEA, Service de Pharmacologie et d Immunologie, DSV DRM, CEA Saclay, 91191 Gif sur Yvette, Cedex, France
    J Chromatogr B Analyt Technol Biomed Life Sci 789:273-81. 2003
    ..The determination of the intracellular ratio of NRTI-TP/dNTP in HIV-infected patients now involves use of the same chromatographic system for simultaneous assay of several analytes...
  9. ncbi High levels of zidovudine (AZT) and its intracellular phosphate metabolites in AZT- and AZT-lamivudine-treated newborns of human immunodeficiency virus-infected mothers
    Lucie Durand-Gasselin
    Service de Pharmacologie et d immunoanalyse, CEA, iBiTecS, 91191 Gif sur Yvette Cedex, France
    Antimicrob Agents Chemother 52:2555-63. 2008
    ..8-fold [P = 0.0415]) than those in males. Unexpectedly, newborns on AZT monotherapy whose mothers' treatment included 3TC displayed residual plasma 3TC and intracellular 3TC-TP levels up to 1 week after birth...
  10. ncbi Emtricitabine: Inhibitor and substrate of multidrug resistance associated protein
    Laurence Bousquet
    CEA, iBiTec S, Service de Pharmacologie et d immunoanalyse, Gif sur Yvette Cedex, France
    Eur J Pharm Sci 35:247-56. 2008
    ..FTC increases intracellular calcein and [(3)H]-vincristine accumulation. Emtricitabine has both inhibitor and substrate characteristics with MRP1 in PBMCs in vitro, and does not interact with PI accumulation...
  11. ncbi Development of a direct assay for measuring intracellular AZT triphosphate in humans peripheral blood mononuclear cells
    François Becher
    Pharmacology and Immunology Unit, CEA, DSV DRM, CEA Saclay, Gif sur Yvette, France
    Anal Chem 74:4220-7. 2002
    ..More than 150 samples/week can be analyzed by one analyst, making this method suitable for routine analysis during clinical studies...
  12. ncbi Combination of tenofovir and emtricitabine plus efavirenz: in vitro modulation of ABC transporter and intracellular drug accumulation
    Laurence Bousquet
    CEA, iBiTecS, Service de Pharmacologie et d immunoanalyse, Laboratoire d Etudes du Métabolisme des Médicaments, Equipe Médicaments et Neuropharmacologie, Gif sur Yvette, France
    Antimicrob Agents Chemother 53:896-902. 2009
    ..This in vitro study reveals a benefit of the combination FTC-TFV-EFV in terms of the intracellular FTC and TFV concentrations and highlights the pharmacological mechanisms that lead to this effect...
  13. ncbi Improved method for the simultaneous determination of d4T, 3TC and ddl intracellular phosphorylated anabolites in human peripheral-blood mononuclear cells using high-performance liquid chromatography/tandem mass spectrometry
    François Becher
    CEA, Pharmacology and Immunology Unit, DSV DRM, CEA Saclay, 91191 Gif sur Yvette Cedex, France
    Rapid Commun Mass Spectrom 16:555-65. 2002
    ..This method can be used simply and routinely on approximately 200 samples per week, using commercially available instruments and with a simple cell lysis as sample treatment...
  14. ncbi Peripheral blood mononuclear cell counting using a DNA-detection-based method
    Henri Benech
    CEA, Service de Pharmacologie et d'Immunologie, DSV/DRM, CEA/Saclay, 91191 Gif-sur-Yvette Cedex, France
    Anal Biochem 330:172-4. 2004
  15. ncbi Direct analysis of valsartan or candesartan in human plasma and urines by on-line solid phase extraction coupled to electrospray tandem mass spectrometry
    Mikael Levi
    CEA, iBiTecS, Service de Pharmacologie et d immunoanalyse, Gif Sur Yvette F 91191, France
    J Chromatogr B Analyt Technol Biomed Life Sci 877:919-26. 2009
    ..2-111% and 0.9-16.9% RSD. Total run time was 4.5min. This on-line clean-up-MS method was found to be robust for the analysis of a high number of samples with unvarying performance...
  16. ncbi Unexpected CD4 cell count decline in patients receiving didanosine and tenofovir-based regimens despite undetectable viral load
    Eugenia Negredo
    Lluita contra la SIDA and Irsicaixa Foundations Germans Trias i Pujol Hospital, Badalona, Spain
    AIDS 18:459-63. 2004
    ..We recently observed a significant CD4 cell count decline in patients receiving didanosine (ddI) 400 mg, tenofovir (TDF) and nevirapine (NVP), despite virological suppression...
  17. ncbi Is stavudine triphosphate a natural metabolite of zidovudine?
    Henri Benech
    Antimicrob Agents Chemother 50:2899; author reply 2899-901. 2006
  18. ncbi Interferon-ribavirin in association with stavudine has no impact on plasma human immunodeficiency virus (HIV) type 1 level in patients coinfected with HIV and hepatitis C virus: a CORIST-ANRS HC1 trial
    Dominique Salmon-Ceron
    Department of Internal Medicine, Cochin Saint Vincent de Paul Hospital, Assistance Publique, Hopitaux de Paris, 75014 Paris France
    Clin Infect Dis 36:1295-304. 2003
    ....