Alain Prochiantz

Summary

Country: France

Publications

  1. ncbi request reprint Protein transduction: from physiology to technology and vice versa
    Alain Prochiantz
    École Normale Supérieure and CNRS, 46, rue d Ulm, 75230 Paris Cedex 05, France
    Adv Drug Deliv Rev 57:491-3. 2005
  2. ncbi request reprint Protein and peptide transduction, twenty years later a happy birthday
    Alain Prochiantz
    Development and Neuropharmacology, UMR CNRS 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Adv Drug Deliv Rev 60:448-51. 2008
  3. ncbi request reprint Can transcription factors function as cell-cell signalling molecules?
    Alain Prochiantz
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 4:814-9. 2003
  4. ncbi request reprint [Messenger proteins]
    A Prochiantz
    CNRS UMR 8542 Ecole normale supérieure, 46, rue d Ulm, 75230 Paris
    J Soc Biol 194:119-23. 2000
  5. ncbi request reprint A peptide fusion a day keeps the aggregates away
    Alain Prochiantz
    CNRS UMR 8542, Ecole Normale Superieure, Paris, France
    Mol Ther 15:226-7. 2007
  6. ncbi request reprint Homeodomain-derived peptides. In and out of the cells
    A Prochiantz
    Ecole Normale Superieure, CNRS UMR 8542, Paris, France
    Ann N Y Acad Sci 886:172-9. 1999
  7. ncbi request reprint A short region of its homeodomain is necessary for engrailed nuclear export and secretion
    A Maizel
    CNRS UMR 8542 and CNRS UMR 8541, Ecole Normale Superieure, Paris Cedex 05, France
    Development 126:3183-90. 1999
  8. ncbi request reprint Getting hydrophilic compounds into cells: lessons from homeopeptides
    A Prochiantz
    CNRS URA 1414, Ecole, Normale, Superieure, Paris, France
    Curr Opin Neurobiol 6:629-34. 1996
  9. doi request reprint Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1: a new genetic model for Parkinson's disease?
    Gwenaëlle Le Pen
    INSERM U894, Laboratory of Pathophysiology of Psychiatric Diseases, University Paris Descartes, Faculty of Medecine Paris Descartes, Sainte Anne Hospital, Paris, France
    Parkinsonism Relat Disord 14:S107-11. 2008
  10. ncbi request reprint Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors
    Isabelle Foucher
    CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Development 130:1867-76. 2003

Collaborators

Detail Information

Publications51

  1. ncbi request reprint Protein transduction: from physiology to technology and vice versa
    Alain Prochiantz
    École Normale Supérieure and CNRS, 46, rue d Ulm, 75230 Paris Cedex 05, France
    Adv Drug Deliv Rev 57:491-3. 2005
  2. ncbi request reprint Protein and peptide transduction, twenty years later a happy birthday
    Alain Prochiantz
    Development and Neuropharmacology, UMR CNRS 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Adv Drug Deliv Rev 60:448-51. 2008
    ..Among these points are bio-disponibility, toxicity and specific addressing to body regions, cell types and intracellular compartments. In brief, beyond this happy birthday, there is still plenty of home work!..
  3. ncbi request reprint Can transcription factors function as cell-cell signalling molecules?
    Alain Prochiantz
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Nat Rev Mol Cell Biol 4:814-9. 2003
    ..But the real excitement stems from the physiological and developmental implications of this mode of signal transduction...
  4. ncbi request reprint [Messenger proteins]
    A Prochiantz
    CNRS UMR 8542 Ecole normale supérieure, 46, rue d Ulm, 75230 Paris
    J Soc Biol 194:119-23. 2000
    ..In addition, the discovery of this type of intercellular signalling has opened the way to the development of widely used peptidic vectors...
  5. ncbi request reprint A peptide fusion a day keeps the aggregates away
    Alain Prochiantz
    CNRS UMR 8542, Ecole Normale Superieure, Paris, France
    Mol Ther 15:226-7. 2007
  6. ncbi request reprint Homeodomain-derived peptides. In and out of the cells
    A Prochiantz
    Ecole Normale Superieure, CNRS UMR 8542, Paris, France
    Ann N Y Acad Sci 886:172-9. 1999
    ..The existence of specific mechanisms for homeoprotein internalization and secretion suggests that this class of transcription factors may have important signaling properties...
  7. ncbi request reprint A short region of its homeodomain is necessary for engrailed nuclear export and secretion
    A Maizel
    CNRS UMR 8542 and CNRS UMR 8541, Ecole Normale Superieure, Paris Cedex 05, France
    Development 126:3183-90. 1999
    ..The present study thus strongly suggests that the regulation of Engrailed nuclear export could play a role not only in Engrailed transcriptional activity but also in its ability to gain access to a secretory compartment...
  8. ncbi request reprint Getting hydrophilic compounds into cells: lessons from homeopeptides
    A Prochiantz
    CNRS URA 1414, Ecole, Normale, Superieure, Paris, France
    Curr Opin Neurobiol 6:629-34. 1996
    ..e. other peptides and oligonucleotides) into the cytoplasm and nucleus of all cell types. This article describes our present understanding of this phenomenon and discusses its potential applications in cell biology...
  9. doi request reprint Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1: a new genetic model for Parkinson's disease?
    Gwenaëlle Le Pen
    INSERM U894, Laboratory of Pathophysiology of Psychiatric Diseases, University Paris Descartes, Faculty of Medecine Paris Descartes, Sainte Anne Hospital, Paris, France
    Parkinsonism Relat Disord 14:S107-11. 2008
    ..Further validation is needed, but these first results suggest that En1+/- mice could provide a promising model for the study of PD...
  10. ncbi request reprint Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factors
    Isabelle Foucher
    CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Development 130:1867-76. 2003
    ..Based on the involvement of conserved domains in both classes of proteins, it is proposed that the interaction between Forkhead box transcription factors and homeoproteins is a general phenomenon...
  11. pmc The transcription factor Engrailed-2 guides retinal axons
    Isabelle Brunet
    CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Nature 438:94-8. 2005
    ..The differential guidance of nasal and temporal axons reported here suggests that En-2 may participate directly in topographic map formation in the vertebrate visual system...
  12. pmc Emx2 homeodomain transcription factor interacts with eukaryotic translation initiation factor 4E (eIF4E) in the axons of olfactory sensory neurons
    Stephane Nedelec
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Neuropharmacology and Development, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:10815-20. 2004
    ..Finally, we show that two other brain-expressed homeoproteins, Otx2 and Engrailed 2, also bind eIF4E, indicating that several homeoproteins may modulate eIF4E functions in the developing and adult nervous system...
  13. pmc Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish
    Brigitte Lesaffre
    Development and Neuropharmacology, CNRS UMR 8542 and Ecole Normale Supérieure, rue d Ulm, 75230 Paris Cedex 05, France
    Neural Dev 2:2. 2007
    ..We have thus developed an approach aimed at blocking Pax6 only in the extracellular milieu of developing zebrafish embryos...
  14. doi request reprint Engrailed protects mouse midbrain dopaminergic neurons against mitochondrial complex I insults
    Daniel Alvarez-Fischer
    Centre de Recherche de l Institut du Cerveau et de la Moelle Epinière University Pierre and Marie Curie Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 975, Paris, France
    Nat Neurosci 14:1260-6. 2011
    ..Therefore, Engrailed is both a survival factor for adult mDA neurons and a regulator of their physiological activity...
  15. ncbi request reprint Dendritic localization and activity-dependent translation of Engrailed1 transcription factor
    Ariel A Di Nardo
    CNRS UMR 8542, Développement et Neuropharmacologie, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
    Mol Cell Neurosci 35:230-6. 2007
    ..Together, these results suggest an activity-dependent role for Engrailed in midbrain dopaminergic neuron physiology...
  16. ncbi request reprint Identification of a signal peptide for unconventional secretion
    Edmond Dupont
    Homeoprotein Cell Biology Group, CNRS UMR 8542, Ecole Normale Superieure, 75230 Paris Cedex 05, France
    J Biol Chem 282:8994-9000. 2007
    ..We have analyzed key mechanisms and demonstrated that this peptide can efficiently cross a tight junction epithelium...
  17. ncbi request reprint The topological role of homeoproteins in the developing central nervous system
    Isabelle Brunet
    Unité Mixte de Recherche 8542, Development and Evolution of the Nervous System Development and Neuropharmacology Group, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
    Trends Neurosci 30:260-7. 2007
    ..Based on these examples, we propose that homeoproteins are not only morphogenetic transcription factors, but also morphogens themselves...
  18. pmc Neurotrophic activity of the Antennapedia homeodomain depends on its specific DNA-binding properties
    I Le Roux
    Centre National de la Recherche Scientifique Unité Recherche Associée 1414, Ecole Normale Superieure, Paris, France
    Proc Natl Acad Sci U S A 90:9120-4. 1993
    ..We propose that homeoproteins might regulate important events in the morphological differentiation of the postmitotic neuron...
  19. doi request reprint Homeoprotein signaling in development, health, and disease: a shaking of dogmas offers challenges and promises from bench to bed
    Julien Spatazza
    Development and Neuropharmacology Group, College de France, Centre for Interdisciplinary Research in Biology, CNRS UMR 7241 INSERM U1050, Labex Memolife, PSL Research University, Paris, France
    Pharmacol Rev 65:90-104. 2013
    ..In conclusion, this review synthesizes 20 years of work on the fundamental and potentially translational aspects of homeoprotein signaling...
  20. pmc Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOP
    Arianna De Toni
    UMR CNRS 8542, ENS, rue d Ulm, 75005 Paris, France
    Neural Dev 3:13. 2008
    ..Here, we have analyzed the function of HOP (Homeodomain only protein) in this stem cell niche and in human glioblastomas...
  21. ncbi request reprint [Protein transduction, from technology to physiology]
    Alain Prochiantz
    CNRS UMR 8542, Ecole Normale Superieure, 46, rue d Ulm, 75230 Paris Cedex 05, France
    J Soc Biol 200:209-12. 2006
    ..Secretion is Golgi independent and requires a small sequence also present in the homeodomain but distinct from the Penetratin sequence. The consequences of this novel signaling mechanism are briefly discussed...
  22. ncbi request reprint Antennapedia homeobox as a signal for the cellular internalization and nuclear addressing of a small exogenous peptide
    F Perez
    CNRS URA 1414, Ecole Normale Superieure, Paris, France
    J Cell Sci 102:717-22. 1992
    ..Beyond its theoretical implications for our current views on cellular interactions, this finding could have technical repercussions on the development of drugs with intracellular targets...
  23. ncbi request reprint Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independent
    D Derossi
    CNRS URA 1414, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    J Biol Chem 271:18188-93. 1996
    ..1995) J. Neurosci. 15, 7158-7167). Therefore, in addition to their physiological implications (Prochiantz, A., and Théodore, L.(1995) BioEssays 17, 39-45), the present results open the way to the molecular design of cellular vectors...
  24. pmc Antennapedia homeobox peptide regulates neural morphogenesis
    A Joliot
    Centre National de la Recherche Scientifique URA 1414, Departement de Biologie, Ecole Normale Superieure, Paris, France
    Proc Natl Acad Sci U S A 88:1864-8. 1991
    ....
  25. ncbi request reprint Homeoproteins as natural Penetratin cargoes with signaling properties
    Alain Joliot
    Homeoprotein Cell Biology Group, UMR CNRS 8542, Ecole Normale Superieure, 46 rue d Ulm 75230 Paris Cedex 05, France
    Adv Drug Deliv Rev 60:608-13. 2008
    ..Although very academic, these two issues are of practical interest for the rational design of new vectors and the identification of unforeseen pathological mechanisms and pharmacological targets...
  26. ncbi request reprint Introduction of exogenous antigens into the MHC class I processing and presentation pathway by Drosophila antennapedia homeodomain primes cytotoxic T cells in vivo
    M P Schutze-Redelmeier
    AIDS Retrovirus Department, Antiviral Cellular Immunity Unit, Pasteur Institute, Paris, France
    J Immunol 157:650-5. 1996
    ..The strategy outlined in this paper provides a new approach for introducing molecules into the MHC class I Ag-presenting pathway. This approach has clear relevance to the design of synthetic peptide-based vaccines...
  27. ncbi request reprint Promoter-specific regulation of gene expression by an exogenously added homedomain that promotes neurite growth
    I Le Roux
    CNRS URA 1414, Ecole Normale Superieure, Paris, France
    FEBS Lett 368:311-4. 1995
    ..Externally added pAntp specifically down-regulates the expression of the reporter gene, suggesting that the neurotrophic effects observed previously are mediated by direct binding of pAntp to homeoprotein target sites...
  28. doi request reprint Penetratin story: an overview
    Edmond Dupont
    Homeoprotein Cell Biology, Ecole normale supérieure and Collège de France, Paris, France
    Methods Mol Biol 683:21-9. 2011
    ..The identification of the Penetratin peptide, summarized in this review, is intimately linked to the study of its parental natural protein...
  29. ncbi request reprint Association of Engrailed homeoproteins with vesicles presenting caveolae-like properties
    A Joliot
    CNRS URA 1414, Ecole Normale Superieure, Paris, France
    Development 124:1865-75. 1997
    ....
  30. ncbi request reprint Messenger proteins: homeoproteins, TAT and others
    A Prochiantz
    CNRS UMR 8542, Ecole Normale Superieure, Paris Cedex 5, 75230, France
    Curr Opin Cell Biol 12:400-6. 2000
    ..These same proteins are often secreted, despite the absence of a signal peptide. Recent studies of this unexpected mode of intercellular signaling have opened the way for biotechnological developments...
  31. ncbi request reprint The third helix of the Antennapedia homeodomain translocates through biological membranes
    D Derossi
    Centre National de la Recherche Scientifique Unité de Recherche Associée 1414, Ecole Normale Superieure, Paris, France
    J Biol Chem 269:10444-50. 1994
    ..Finally, substitution of two tryptophans by two phenylalanines strongly diminishes translocation, raising the possibility that the internalization of the third helix is not solely based on its general hydrophobicity...
  32. ncbi request reprint Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1
    Laure Sonnier
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Development and Evolution of the Nervous System, Group Development and Neuropharmacology, Ecole Normale Superieure, 75230 Paris Cedex 05, France
    J Neurosci 27:1063-71. 2007
    ..They also suggest that mice lacking only one En1 allele could provide a novel model for the study of diseases associated with progressive DA cell death...
  33. ncbi request reprint Hoxa5 overexpression correlates with IGFBP1 upregulation and postnatal dwarfism: evidence for an interaction between Hoxa5 and Forkhead box transcription factors
    Isabelle Foucher
    CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
    Development 129:4065-74. 2002
    ....
  34. ncbi request reprint Transduction peptides: from technology to physiology
    Alain Joliot
    Homeoprotein Cell Biology Group, CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris, Cedex 05 France
    Nat Cell Biol 6:189-96. 2004
    ....
  35. ncbi request reprint Soluble form of amyloid precursor protein regulates proliferation of progenitors in the adult subventricular zone
    Isabelle Caille
    CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
    Development 131:2173-81. 2004
    ..These results reveal a new function for sAPP as a regulator of SVZ progenitor proliferation in the adult central nervous system...
  36. doi request reprint Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptides
    Alain Prochiantz
    Ecole normale supérieure and Collège de France, Paris, France
    Methods Mol Biol 683:249-57. 2011
    ..In this chapter, I describe some of these functions and propose that this class of signaling molecules, in particular homeoproteins, may also be used as therapeutic agents...
  37. pmc Otx2 binding to perineuronal nets persistently regulates plasticity in the mature visual cortex
    Marine Beurdeley
    College de France, Center for Interdisciplinary Research in Biology, F 75005 Paris, France
    J Neurosci 32:9429-37. 2012
    ..The pharmacological use of the Otx2 GAG binding domain offers a novel, potent therapeutic tool with which to restore cortical plasticity in the mature brain...
  38. doi request reprint Cell non-autonomous functions of homeoproteins in neuroprotection in the brain
    Rajiv L Joshi
    Development and Neuropharmacology Group, Centre for Interdisciplinary Research in Biology CIRB, CNRS UMR 7241, INSERM U 1050, College de France, Paris, France
    FEBS Lett 585:1573-8. 2011
    ..The homeoprotein Otx2 promotes the survival of injured adult RGCs both in vitro and in vivo. These examples raise the possibility that homeoproteins may provide neuroprotection to neurons vulnerable in other neurodegenerative diseases...
  39. ncbi request reprint [Transduction peptides, the useful face of a new signaling mechanism]
    Alain Joliot
    Equipe biologie cellulaire des homéoprotéines, CNRS UMR 8542, Ecole Normale Superieure, 46, rue d Ulm, 75230 Paris Cedex 05, France
    Med Sci (Paris) 21:309-14. 2005
    ....
  40. ncbi request reprint SET protein (TAF1beta, I2PP2A) is involved in neuronal apoptosis induced by an amyloid precursor protein cytoplasmic subdomain
    A Madeira
    INSERM U 573, Centre Paul Broca, Paris, France
    FASEB J 19:1905-7. 2005
    ..Taken together, these results suggest that SET is part of a neuronal apoptotic pathway related to Alzheimer's disease and provides a new entry in the analysis of this pathology...
  41. pmc Evolution of the nervous system: a critical evaluation of how genetic changes translate into morphological changes
    Alain Prochiantz
    College de France, Centre National de la Recherche Scientifique, Ecole Normale Superieure, Paris, France
    Dialogues Clin Neurosci 12:457-62. 2010
    ....
  42. ncbi request reprint The neuronal microtubule-associated protein 1B is under homeoprotein transcriptional control
    M L Montesinos
    , , 75230 Paris, Cedex 05 France
    J Neurosci 21:3350-9. 2001
    ..It is thus proposed that MAP1B, a cytoskeleton protein involved in neuronal growth and regeneration, is under homeoprotein transcriptional regulation...
  43. ncbi request reprint A short cytoplasmic domain of the amyloid precursor protein induces apoptosis in vitro and in vivo
    E Bertrand
    Centre National de la Recherche de Scientifique UMR 8542, , Paris, France
    Mol Cell Neurosci 18:503-11. 2001
    ..Taken together, this report suggests the existence of a new mechanism contributing to Alzheimer's Disease-associated cell death...
  44. ncbi request reprint The amyloid precursor protein interacts with Go heterotrimeric protein within a cell compartment specialized in signal transduction
    E Brouillet
    Centre National de la Recherche Scientifique, Unité de Recherche Associée 1414, Ecole Normale Superieure, 75230 Paris Cedex 05, France
    J Neurosci 19:1717-27. 1999
    ..The regulation of Galphao GTPase activity by betaAPP in a compartment specialized in signaling may have important consequences for our understanding of the physiopathological functions of betaAPP...
  45. ncbi request reprint Tryptophan fluorescence study of the interaction of penetratin peptides with model membranes
    Bart Christiaens
    Laboratory for Lipoprotein Chemistry, Ghent University, Belgium
    Eur J Biochem 269:2918-26. 2002
    ....
  46. ncbi request reprint For protein transduction, chemistry can win over biology
    Alain Prochiantz
    Nat Methods 4:119-20. 2007
  47. ncbi request reprint Membrane interaction and cellular internalization of penetratin peptides
    Bart Christiaens
    Department of Biochemistry of the Ghent University, Belgium
    Eur J Biochem 271:1187-97. 2004
    ..None of the penetratin variants was haemolytic or cytotoxic...
  48. ncbi request reprint Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by Pax6
    Alexander von Holst
    Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, D 44780 Bochum, Germany
    J Biol Chem 282:9172-81. 2007
    ..These findings suggest a functional significance for individual Tnc isoforms in neural stem/progenitor cells...
  49. ncbi request reprint Engrailed homeoprotein secretion is a regulated process
    Alexis Maizel
    Biologie cellulaire des homéoprotéines, CNRS UMR8542 Ecole Normale Supérieure 46 rue d Ulm F 75005 Paris, France
    Development 129:3545-53. 2002
    ..This indicates that intercellular transfer of Engrailed 2 is a highly regulated process...
  50. doi request reprint Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticity
    Sayaka Sugiyama
    Lab for Neuronal Circuit Development, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
    Cell 134:508-20. 2008
    ..Thus, the experience-dependent transfer of a homeoprotein may establish the physiological milieu for postnatal plasticity of a neural circuit...
  51. ncbi request reprint Morphogenetic gradients and the stability of boundaries between neighboring morphogenetic regions
    Victor Kasatkin
    Department of Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel
    Bull Math Biol 70:156-78. 2008
    ..This formula can be used to study the noise intrinsic effect on the boundary position between morphogenetic regions, which can be at the origin of interindividual variations...