Research Topics
Genomes and Genes
| Alain ProchiantzSummaryCountry: France Publications
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Publications
Protein and peptide transduction, twenty years later a happy birthdayAlain Prochiantz
Development and Neuropharmacology, UMR CNRS 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
Adv Drug Deliv Rev 60:448-51. 2008..Among these points are bio-disponibility, toxicity and specific addressing to body regions, cell types and intracellular compartments. In brief, beyond this happy birthday, there is still plenty of home work!..- Protein transduction: from physiology to technology and vice versaAlain Prochiantz
, 46, rue d'Ulm, 75230 Paris Cedex 05, France
Adv Drug Deliv Rev 57:491-3. 2005 - A peptide fusion a day keeps the aggregates awayAlain Prochiantz
CNRS UMR 8542, Ecole Normale Superieure, Paris, France
Mol Ther 15:226-7. 2007 - [Messenger proteins]A Prochiantz
CNRS UMR 8542 Ecole normale supérieure, 46, rue d Ulm, 75230 Paris
J Soc Biol 194:119-23. 2000..In addition, the discovery of this type of intercellular signalling has opened the way to the development of widely used peptidic vectors... - Can transcription factors function as cell-cell signalling molecules?Alain Prochiantz
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
Nat Rev Mol Cell Biol 4:814-9. 2003..But the real excitement stems from the physiological and developmental implications of this mode of signal transduction... - Homeodomain-derived peptides. In and out of the cellsA Prochiantz
Ecole Normale Superieure, CNRS UMR 8542, Paris, France
Ann N Y Acad Sci 886:172-9. 1999..The existence of specific mechanisms for homeoprotein internalization and secretion suggests that this class of transcription factors may have important signaling properties... - A short region of its homeodomain is necessary for engrailed nuclear export and secretionA Maizel
CNRS UMR 8542 and CNRS UMR 8541, Ecole Normale Superieure, Paris Cedex 05, France
Development 126:3183-90. 1999..The present study thus strongly suggests that the regulation of Engrailed nuclear export could play a role not only in Engrailed transcriptional activity but also in its ability to gain access to a secretory compartment... - Getting hydrophilic compounds into cells: lessons from homeopeptidesA Prochiantz
CNRS URA 1414, Ecole, Normale, Superieure, Paris, France
Curr Opin Neurobiol 6:629-34. 1996..e. other peptides and oligonucleotides) into the cytoplasm and nucleus of all cell types. This article describes our present understanding of this phenomenon and discusses its potential applications in cell biology... 
Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1: a new genetic model for Parkinson's disease?Gwenaëlle Le Pen
INSERM U894, Laboratory of Pathophysiology of Psychiatric Diseases, University Paris Descartes, Faculty of Medecine Paris Descartes, Sainte Anne Hospital, Paris, France
Parkinsonism Relat Disord 14:S107-11. 2008..Further validation is needed, but these first results suggest that En1+/- mice could provide a promising model for the study of PD...- Joint regulation of the MAP1B promoter by HNF3beta/Foxa2 and Engrailed is the result of a highly conserved mechanism for direct interaction of homeoproteins and Fox transcription factorsIsabelle Foucher
CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
Development 130:1867-76. 2003..Based on the involvement of conserved domains in both classes of proteins, it is proposed that the interaction between Forkhead box transcription factors and homeoproteins is a general phenomenon... - The transcription factor Engrailed-2 guides retinal axonsIsabelle Brunet
CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
Nature 438:94-8. 2005..The differential guidance of nasal and temporal axons reported here suggests that En-2 may participate directly in topographic map formation in the vertebrate visual system... 
Emx2 homeodomain transcription factor interacts with eukaryotic translation initiation factor 4E (eIF4E) in the axons of olfactory sensory neuronsStephane Nedelec
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Neuropharmacology and Development, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
Proc Natl Acad Sci U S A 101:10815-20. 2004..Finally, we show that two other brain-expressed homeoproteins, Otx2 and Engrailed 2, also bind eIF4E, indicating that several homeoproteins may modulate eIF4E functions in the developing and adult nervous system...
Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafishBrigitte Lesaffre
Development and Neuropharmacology, CNRS UMR 8542 and Ecole Normale Supérieure, rue d Ulm, 75230 Paris Cedex 05, France
Neural Dev 2:2. 2007..We have thus developed an approach aimed at blocking Pax6 only in the extracellular milieu of developing zebrafish embryos...- Engrailed protects mouse midbrain dopaminergic neurons against mitochondrial complex I insultsDaniel Alvarez-Fischer
Centre de Recherche de l Institut du Cerveau et de la Moelle Epinière University Pierre and Marie Curie Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche S 975, Paris, France
Nat Neurosci 14:1260-6. 2011..Therefore, Engrailed is both a survival factor for adult mDA neurons and a regulator of their physiological activity... - Dendritic localization and activity-dependent translation of Engrailed1 transcription factorAriel A Di Nardo
CNRS UMR 8542, Développement et Neuropharmacologie, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
Mol Cell Neurosci 35:230-6. 2007..Together, these results suggest an activity-dependent role for Engrailed in midbrain dopaminergic neuron physiology... - Identification of a signal peptide for unconventional secretionEdmond Dupont
Homeoprotein Cell Biology Group, CNRS UMR 8542, Ecole Normale Superieure, 75230 Paris Cedex 05, France
J Biol Chem 282:8994-9000. 2007..We have analyzed key mechanisms and demonstrated that this peptide can efficiently cross a tight junction epithelium... - The topological role of homeoproteins in the developing central nervous systemIsabelle Brunet
Unité Mixte de Recherche 8542, Development and Evolution of the Nervous System Development and Neuropharmacology Group, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
Trends Neurosci 30:260-7. 2007..Based on these examples, we propose that homeoproteins are not only morphogenetic transcription factors, but also morphogens themselves... - Neurotrophic activity of the Antennapedia homeodomain depends on its specific DNA-binding propertiesI Le Roux
Centre National de la Recherche Scientifique Unité Recherche Associée 1414, Ecole Normale Superieure, Paris, France
Proc Natl Acad Sci U S A 90:9120-4. 1993..We propose that homeoproteins might regulate important events in the morphological differentiation of the postmitotic neuron... - [Protein transduction, from technology to physiology]Alain Prochiantz
CNRS UMR 8542, Ecole Normale Superieure, 46, rue d Ulm, 75230 Paris Cedex 05, France
J Soc Biol 200:209-12. 2006..Secretion is Golgi independent and requires a small sequence also present in the homeodomain but distinct from the Penetratin sequence. The consequences of this novel signaling mechanism are briefly discussed... - Regulation of survival in adult hippocampal and glioblastoma stem cell lineages by the homeodomain-only protein HOPArianna De Toni
UMR CNRS 8542, ENS, rue d Ulm, 75005 Paris, France
Neural Dev 3:13. 2008..Here, we have analyzed the function of HOP (Homeodomain only protein) in this stem cell niche and in human glioblastomas... - Antennapedia homeobox as a signal for the cellular internalization and nuclear addressing of a small exogenous peptideF Perez
CNRS URA 1414, Ecole Normale Superieure, Paris, France
J Cell Sci 102:717-22. 1992..Beyond its theoretical implications for our current views on cellular interactions, this finding could have technical repercussions on the development of drugs with intracellular targets... - Cell internalization of the third helix of the Antennapedia homeodomain is receptor-independentD Derossi
CNRS URA 1414, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
J Biol Chem 271:18188-93. 1996..1995) J. Neurosci. 15, 7158-7167). Therefore, in addition to their physiological implications (Prochiantz, A., and Théodore, L.(1995) BioEssays 17, 39-45), the present results open the way to the molecular design of cellular vectors... - Antennapedia homeobox peptide regulates neural morphogenesisA Joliot
Centre National de la Recherche Scientifique URA 1414, Departement de Biologie, Ecole Normale Superieure, Paris, France
Proc Natl Acad Sci U S A 88:1864-8. 1991.... - Homeoproteins as natural Penetratin cargoes with signaling propertiesAlain Joliot
Homeoprotein Cell Biology Group, UMR CNRS 8542, Ecole Normale Superieure, 46 rue d Ulm 75230 Paris Cedex 05, France
Adv Drug Deliv Rev 60:608-13. 2008..Although very academic, these two issues are of practical interest for the rational design of new vectors and the identification of unforeseen pathological mechanisms and pharmacological targets... - Introduction of exogenous antigens into the MHC class I processing and presentation pathway by Drosophila antennapedia homeodomain primes cytotoxic T cells in vivoM P Schutze-Redelmeier
AIDS Retrovirus Department, Antiviral Cellular Immunity Unit, Pasteur Institute, Paris, France
J Immunol 157:650-5. 1996..The strategy outlined in this paper provides a new approach for introducing molecules into the MHC class I Ag-presenting pathway. This approach has clear relevance to the design of synthetic peptide-based vaccines... - Promoter-specific regulation of gene expression by an exogenously added homedomain that promotes neurite growthI Le Roux
CNRS URA 1414, Ecole Normale Superieure, Paris, France
FEBS Lett 368:311-4. 1995..Externally added pAntp specifically down-regulates the expression of the reporter gene, suggesting that the neurotrophic effects observed previously are mediated by direct binding of pAntp to homeoprotein target sites... - Penetratin story: an overviewEdmond Dupont
Homeoprotein Cell Biology, Ecole normale supérieure and Collège de France, Paris, France
Methods Mol Biol 683:21-9. 2011..The identification of the Penetratin peptide, summarized in this review, is intimately linked to the study of its parental natural protein... - Messenger proteins: homeoproteins, TAT and othersA Prochiantz
CNRS UMR 8542, Ecole Normale Superieure, Paris Cedex 5, 75230, France
Curr Opin Cell Biol 12:400-6. 2000..These same proteins are often secreted, despite the absence of a signal peptide. Recent studies of this unexpected mode of intercellular signaling have opened the way for biotechnological developments... - Association of Engrailed homeoproteins with vesicles presenting caveolae-like propertiesA Joliot
CNRS URA 1414, Ecole Normale Superieure, Paris, France
Development 124:1865-75. 1997.... - The third helix of the Antennapedia homeodomain translocates through biological membranesD Derossi
Centre National de la Recherche Scientifique Unité de Recherche Associée 1414, Ecole Normale Superieure, Paris, France
J Biol Chem 269:10444-50. 1994..Finally, substitution of two tryptophans by two phenylalanines strongly diminishes translocation, raising the possibility that the internalization of the third helix is not solely based on its general hydrophobicity... - Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1Laure Sonnier
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Development and Evolution of the Nervous System, Group Development and Neuropharmacology, Ecole Normale Superieure, 75230 Paris Cedex 05, France
J Neurosci 27:1063-71. 2007..They also suggest that mice lacking only one En1 allele could provide a novel model for the study of diseases associated with progressive DA cell death... - Homeoprotein intercellular transfer, the hidden face of cell-penetrating peptidesAlain Prochiantz
Ecole normale supérieure and Collège de France, Paris, France
Methods Mol Biol 683:249-57. 2011..In this chapter, I describe some of these functions and propose that this class of signaling molecules, in particular homeoproteins, may also be used as therapeutic agents... - Soluble form of amyloid precursor protein regulates proliferation of progenitors in the adult subventricular zoneIsabelle Caille
CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75005 Paris, France
Development 131:2173-81. 2004..These results reveal a new function for sAPP as a regulator of SVZ progenitor proliferation in the adult central nervous system... - Hoxa5 overexpression correlates with IGFBP1 upregulation and postnatal dwarfism: evidence for an interaction between Hoxa5 and Forkhead box transcription factorsIsabelle Foucher
CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris Cedex 05, France
Development 129:4065-74. 2002.... - Transduction peptides: from technology to physiologyAlain Joliot
Homeoprotein Cell Biology Group, CNRS UMR 8542, Ecole Normale Superieure, 46 rue d Ulm, 75230 Paris, Cedex 05 France
Nat Cell Biol 6:189-96. 2004.... - Otx2 binding to perineuronal nets persistently regulates plasticity in the mature visual cortexMarine Beurdeley
College de France, Center for Interdisciplinary Research in Biology, F 75005 Paris, France
J Neurosci 32:9429-37. 2012..The pharmacological use of the Otx2 GAG binding domain offers a novel, potent therapeutic tool with which to restore cortical plasticity in the mature brain... - Cell non-autonomous functions of homeoproteins in neuroprotection in the brainRajiv L Joshi
Development and Neuropharmacology Group, Centre for Interdisciplinary Research in Biology CIRB, CNRS UMR 7241, INSERM U 1050, College de France, Paris, France
FEBS Lett 585:1573-8. 2011..The homeoprotein Otx2 promotes the survival of injured adult RGCs both in vitro and in vivo. These examples raise the possibility that homeoproteins may provide neuroprotection to neurons vulnerable in other neurodegenerative diseases... - [Transduction peptides, the useful face of a new signaling mechanism]Alain Joliot
Equipe biologie cellulaire des homéoprotéines, CNRS UMR 8542, Ecole Normale Superieure, 46, rue d Ulm, 75230 Paris Cedex 05, France
Med Sci (Paris) 21:309-14. 2005.... - SET protein (TAF1beta, I2PP2A) is involved in neuronal apoptosis induced by an amyloid precursor protein cytoplasmic subdomainA Madeira
INSERM U 573, Centre Paul Broca, Paris, France
FASEB J 19:1905-7. 2005..Taken together, these results suggest that SET is part of a neuronal apoptotic pathway related to Alzheimer's disease and provides a new entry in the analysis of this pathology... - Evolution of the nervous system: a critical evaluation of how genetic changes translate into morphological changesAlain Prochiantz
College de France, Centre National de la Recherche Scientifique, Ecole Normale Superieure, Paris, France
Dialogues Clin Neurosci 12:457-62. 2010.... - The neuronal microtubule-associated protein 1B is under homeoprotein transcriptional controlM L Montesinos
, , 75230 Paris, Cedex 05 France
J Neurosci 21:3350-9. 2001..It is thus proposed that MAP1B, a cytoskeleton protein involved in neuronal growth and regeneration, is under homeoprotein transcriptional regulation... - A short cytoplasmic domain of the amyloid precursor protein induces apoptosis in vitro and in vivoE Bertrand
Centre National de la Recherche de Scientifique UMR 8542, , Paris, France
Mol Cell Neurosci 18:503-11. 2001..Taken together, this report suggests the existence of a new mechanism contributing to Alzheimer's Disease-associated cell death... - The amyloid precursor protein interacts with Go heterotrimeric protein within a cell compartment specialized in signal transductionE Brouillet
Centre National de la Recherche Scientifique, Unité de Recherche Associée 1414, Ecole Normale Superieure, 75230 Paris Cedex 05, France
J Neurosci 19:1717-27. 1999..The regulation of Galphao GTPase activity by betaAPP in a compartment specialized in signaling may have important consequences for our understanding of the physiopathological functions of betaAPP... - For protein transduction, chemistry can win over biologyAlain Prochiantz
Nat Methods 4:119-20. 2007 - Tryptophan fluorescence study of the interaction of penetratin peptides with model membranesBart Christiaens
Laboratory for Lipoprotein Chemistry, Ghent University, Belgium
Eur J Biochem 269:2918-26. 2002.... - Morphogenetic gradients and the stability of boundaries between neighboring morphogenetic regionsVictor Kasatkin
Department of Mathematics, Weizmann Institute of Science, Rehovot 76100, Israel
Bull Math Biol 70:156-78. 2008..This formula can be used to study the noise intrinsic effect on the boundary position between morphogenetic regions, which can be at the origin of interindividual variations... - Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by Pax6Alexander von Holst
Department of Cell Morphology and Molecular Neurobiology, Ruhr University Bochum, D 44780 Bochum, Germany
J Biol Chem 282:9172-81. 2007..These findings suggest a functional significance for individual Tnc isoforms in neural stem/progenitor cells... - Engrailed homeoprotein secretion is a regulated processAlexis Maizel
, , France
Development 129:3545-53. 2002..This indicates that intercellular transfer of Engrailed 2 is a highly regulated process... - Membrane interaction and cellular internalization of penetratin peptidesBart Christiaens
Department of Biochemistry of the Ghent University, Belgium
Eur J Biochem 271:1187-97. 2004..None of the penetratin variants was haemolytic or cytotoxic... - Experience-dependent transfer of Otx2 homeoprotein into the visual cortex activates postnatal plasticitySayaka Sugiyama
Lab for Neuronal Circuit Development, RIKEN Brain Science Institute, 2 1 Hirosawa, Wako Shi, Saitama 351 0198, Japan
Cell 134:508-20. 2008..Thus, the experience-dependent transfer of a homeoprotein may establish the physiological milieu for postnatal plasticity of a neural circuit...