Claude Prigent

Summary

Country: France

Publications

  1. ncbi request reprint Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure
    Claude Prigent
    Groupe Cycle Cellulaire, UMR6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR97 Génomique Fonctionnelle et Santé, 35043 Rennes Cedex, France
    Exp Cell Res 303:1-13. 2005
  2. pmc FBXW7/hCDC4 controls glioma cell proliferation in vitro and is a prognostic marker for survival in glioblastoma patients
    Martin Hagedorn
    INSERM, E0113, Mécanismes Moléculaires de l Angiogenèse, Talence, F 33405, France
    Cell Div 2:9. 2007
  3. pmc Regulation of the Aurora-A gene following topoisomerase I inhibition: implication of the Myc transcription factor
    Sandy Courapied
    Cancer Center Paul Papin, INSERM U892, Angers, France
    Mol Cancer 9:205. 2010
  4. ncbi request reprint Phosphorylation of serine 10 in histone H3, what for?
    Claude Prigent
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS, 250 Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    J Cell Sci 116:3677-85. 2003
  5. ncbi request reprint Two mammalian mitotic aurora kinases: who's who?
    S Descamps
    the Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    Sci STKE 2001:pe1. 2001
  6. ncbi request reprint Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
    Jean Guy Delcros
    CNRS UMR 6061 Génétique et Développement, Groupe Cycle Cellulaire, Faculte de Medecine, IFR 140 Génomique Fonctionnelle et Santé, Universite de Rennes I, 2 Avenue du Pr Leon Bernard, CS 34317, F 35043 Rennes Cedex, France
    J Cell Sci 119:4431-41. 2006
  7. pmc The PITSLRE/CDK11p58 protein kinase promotes centrosome maturation and bipolar spindle formation
    Clotilde Petretti
    CNRS UMR 6061 Université de Rennes I, Equipe labellisée Ligue Nationale Contre le Cancer, IFR140 GFAS, Faculte de Medecine, France
    EMBO Rep 7:418-24. 2006
  8. ncbi request reprint Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation
    Gaetan Pascreau
    Groupe Cycle Cellulaire, UMR6061 Génétique et Développement, CNRS, Universite de Rennes 1, IFR97 Génomique Fonctionnelle, Faculte de Medecine, 2 Av du Pr Leon Bernard, 35043 Rennes Cedex, France
    J Biol Chem 280:13415-23. 2005
  9. doi request reprint Aurora-A kinase Ser349 phosphorylation is required during Xenopus laevis oocyte maturation
    Gaetan Pascreau
    CNRS UMR6061 Génétique et Développement, Groupe Cycle Cellulaire, IFR 140 GFAS, Faculte de Medecine, Universite Rennes 1, 2 Av du Pr Leon Bernard, 35043 Rennes Cedex, France
    Dev Biol 317:523-30. 2008
  10. ncbi request reprint Aurora-A overexpression leads to override of the microtubule-kinetochore attachment checkpoint
    Stéphanie Dutertre
    Groupe Cycle Cellulaire, UMR6061 CNRS Université de Rennes 1, Faculte de Medecine, 2 Avenue du Pr Léon Bernard CS 34317, 35043 Rennes Cedex, France
    Mol Interv 3:127-30. 2003

Collaborators

Detail Information

Publications44

  1. ncbi request reprint Drosophila Nek2 protein kinase knockdown leads to centrosome maturation defects while overexpression causes centrosome fragmentation and cytokinesis failure
    Claude Prigent
    Groupe Cycle Cellulaire, UMR6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR97 Génomique Fonctionnelle et Santé, 35043 Rennes Cedex, France
    Exp Cell Res 303:1-13. 2005
    ..We discuss potential roles for DmNek2 in maintaining centrosome integrity in mitosis, during cytokinesis, and consequently for the fidelity of chromosome segregation...
  2. pmc FBXW7/hCDC4 controls glioma cell proliferation in vitro and is a prognostic marker for survival in glioblastoma patients
    Martin Hagedorn
    INSERM, E0113, Mécanismes Moléculaires de l Angiogenèse, Talence, F 33405, France
    Cell Div 2:9. 2007
    ..Any of the identified targets, Cyclin E, c-Myc, c-Jun, Notch1/4 and Aurora-A may have oncogenic properties when accumulated in tumors with FBXW7 loss...
  3. pmc Regulation of the Aurora-A gene following topoisomerase I inhibition: implication of the Myc transcription factor
    Sandy Courapied
    Cancer Center Paul Papin, INSERM U892, Angers, France
    Mol Cancer 9:205. 2010
    ..We therefore propose the hypothesis that the coordinated overexpression of Myc and Aurora-A, together with a downregulation of Mad and Miz-1 should be tested as a prognosis signature of poor responses to topoisomerase I inhibitors...
  4. ncbi request reprint Phosphorylation of serine 10 in histone H3, what for?
    Claude Prigent
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS, 250 Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    J Cell Sci 116:3677-85. 2003
    ..What is the kinase and under what circumstances does Ser10 becomes phosphorylated? Most importantly, what are the consequences of phosphorylation of this residue?..
  5. ncbi request reprint Two mammalian mitotic aurora kinases: who's who?
    S Descamps
    the Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    Sci STKE 2001:pe1. 2001
    ..The Perspective by Descamps and Prigent delves into the latest observations on aurora kinases in humans and the specific roles of each kinase within the process of mitosis...
  6. ncbi request reprint Dynactin targets Pavarotti-KLP to the central spindle during anaphase and facilitates cytokinesis in Drosophila S2 cells
    Jean Guy Delcros
    CNRS UMR 6061 Génétique et Développement, Groupe Cycle Cellulaire, Faculte de Medecine, IFR 140 Génomique Fonctionnelle et Santé, Universite de Rennes I, 2 Avenue du Pr Leon Bernard, CS 34317, F 35043 Rennes Cedex, France
    J Cell Sci 119:4431-41. 2006
    ..The contribution of the dynein-dynactin complex to synchronous chromosome segregation and cytokinesis is discussed...
  7. pmc The PITSLRE/CDK11p58 protein kinase promotes centrosome maturation and bipolar spindle formation
    Clotilde Petretti
    CNRS UMR 6061 Université de Rennes I, Equipe labellisée Ligue Nationale Contre le Cancer, IFR140 GFAS, Faculte de Medecine, France
    EMBO Rep 7:418-24. 2006
    ..This work demonstrates for the first time the role of CDK11(p58) in centrosome maturation and bipolar spindle morphogenesis...
  8. ncbi request reprint Phosphorylation of maskin by Aurora-A participates in the control of sequential protein synthesis during Xenopus laevis oocyte maturation
    Gaetan Pascreau
    Groupe Cycle Cellulaire, UMR6061 Génétique et Développement, CNRS, Universite de Rennes 1, IFR97 Génomique Fonctionnelle, Faculte de Medecine, 2 Av du Pr Leon Bernard, 35043 Rennes Cedex, France
    J Biol Chem 280:13415-23. 2005
    ..This result strongly suggests that phosphorylation of maskin by Aurora-A prevents meiosis II proteins from being produced during meiosis I...
  9. doi request reprint Aurora-A kinase Ser349 phosphorylation is required during Xenopus laevis oocyte maturation
    Gaetan Pascreau
    CNRS UMR6061 Génétique et Développement, Groupe Cycle Cellulaire, IFR 140 GFAS, Faculte de Medecine, Universite Rennes 1, 2 Av du Pr Leon Bernard, 35043 Rennes Cedex, France
    Dev Biol 317:523-30. 2008
    ..Since phosphorylation of Ser349 is responsible for a decrease in kinase activity, our results suggest that a down-regulation of Aurora-A activity involving Ser349 phosphorylation is required in the process of maturation...
  10. ncbi request reprint Aurora-A overexpression leads to override of the microtubule-kinetochore attachment checkpoint
    Stéphanie Dutertre
    Groupe Cycle Cellulaire, UMR6061 CNRS Université de Rennes 1, Faculte de Medecine, 2 Avenue du Pr Léon Bernard CS 34317, 35043 Rennes Cedex, France
    Mol Interv 3:127-30. 2003
    ..Thus, the design of new drugs that specifically target aurora-A rather than other checkpoint proteins might alleviate the resistance to Taxol-like clinical therapeutics observed in some tumors...
  11. doi request reprint Aurora-C interacts with and phosphorylates the transforming acidic coiled-coil 1 protein
    Jean Charles Gabillard
    CNRS UMR 6061, Institut Génétique et Développement, IFR 140, UEB Université Rennes 1, 2 Avenue du Pr Leon Bernard, Rennes Cedex, France
    Biochem Biophys Res Commun 408:647-53. 2011
    ....
  12. ncbi request reprint Aurora kinases, aneuploidy and cancer, a coincidence or a real link?
    Régis Giet
    CNRS UMR6061 Université de Rennes 1, Groupe Cycle Cellulaire, Equipe Labellisée LNCC, Universite de Rennes 1, IFR140 GFAS, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 3417, Rennes Cedex, France
    Trends Cell Biol 15:241-50. 2005
    ....
  13. doi request reprint [Centrosomes, mitotic spindle and cancer: find the odd one out!]
    Pierre Romé
    Institut de Génétique et Développement de Rennes, UMR 6061, Faculte de Medecine, professeur Léon Bernard, CS 34317, 35043 Rennes Cedex, France
    Med Sci (Paris) 26:377-83. 2010
    ..It is thus understandable why any quantitative or qualitative dysfunction of centrioles contributes to genomic -instability and thus -tumorigenesis...
  14. ncbi request reprint The non-catalytic domain of the Xenopus laevis auroraA kinase localises the protein to the centrosome
    R Giet
    Groupe Cycle Cellulaire, , , , , , CS 34317, 35043 Rennes Cedex, France
    J Cell Sci 114:2095-104. 2001
    ....
  15. ncbi request reprint The absence of p53 aggravates polyploidy and centrosome number abnormality induced by Aurora-C overexpression
    Stéphanie Dutertre
    CNRS UMR6061, Genetique et Developpement, Universite de Rennes 1, IFR140, Groupe Cycle Cellulaire, Equipe labellisée Ligue Nationale Contre le Cancer, Faculte de Medecine, Rennes, France
    Cell Cycle 4:1783-7. 2005
    ....
  16. pmc Overexpression of active Aurora-C kinase results in cell transformation and tumour formation
    Jabbar Khan
    CNRS, UMR 6061, Institut Génétique et Développement de Rennes, Rennes, France
    PLoS ONE 6:e26512. 2011
    ..Interestingly enough tumor aggressiveness was positively correlated with the quantity of active kinase, making Aurora-C a potential anti-cancer therapeutic target...
  17. ncbi request reprint Aurora A and mitotic commitment
    Claude Prigent
    CNRS UMR6061 University of Rennes I, Equipe labellisée Ligue Nationale Contre le Cancer, 2 Avenue du Pr Leon Bernard, 35043 cedex, Rennes, France
    Cell 114:531-2. 2003
    ..A remarkable study published in this issue of Cell reveals a key role of Aurora A protein kinase in G2/M progression. To achieve this role, Aurora A acts in conjunction with the LIM protein Ajuba, which functions as an activating factor...
  18. ncbi request reprint Spatio-temporal expression patterns of aurora kinases a, B, and C and cytoplasmic polyadenylation-element-binding protein in bovine oocytes during meiotic maturation
    Svetlana Uzbekova
    INRA, UMR85 Physiologie de Reproduction et des Comportements, CNRS, UMR6175, Universite de Tours, Haras Nationaux, 37380 Nouzilly, France
    Biol Reprod 78:218-33. 2008
    ....
  19. ncbi request reprint Phosphorylation of histone and histone-like proteins by aurora kinases during mitosis
    Gaetan Pascreau
    Groupe Cycle Cellulaire, UMR6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes, France
    Prog Cell Cycle Res 5:369-74. 2003
    ..This finding has stimulated functional studies of the role(s) of Aurora kinases and H3 phosphorylation during mitosis, which are reviewed in this chapter...
  20. ncbi request reprint Identification of a functional destruction box in the Xenopus laevis aurora-A kinase pEg2
    Y Arlot-Bonnemains
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    FEBS Lett 508:149-52. 2001
    ..The sequence RxxL corresponding to the pEg2 functional destruction box has been conserved throughout evolution in all aurora kinases including aurora-A, -B and -C...
  21. ncbi request reprint The Protein Kinase Resource: everything you always wanted to know about protein kinases but were afraid to ask
    Clotilde Petretti
    Groupe Cycle Cellulaire, Equipe labellisée Ligue Nationale Contre le Cancer, UMR 6061 Génétique et Développement, CNRS, Universite de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 35043 Rennes Cedex, France
    Biol Cell 97:113-8. 2005
    ..This review describes such a web resource, 'The Protein Kinase Resource' (http://pkr.sdsc.edu/html/index.shtml), which serves as a repository for cellular and molecular data on protein kinases...
  22. ncbi request reprint Cell cycle. A trigger for centrosome duplication
    Yannick Arlot-Bonnemains
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, CS 34317, 35043 Rennes Cedex, France
    Science 295:455-6. 2002
  23. ncbi request reprint Centrosome separation: respective role of microtubules and actin filaments
    Rustem Uzbekov
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR 97 génomique et santé Faculté de médecine, 2, avenue du professeur Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    Biol Cell 94:275-88. 2002
    ..Our results clearly show that the actin microfilaments participate in centrosome separation at the G2/M transition and work in synergy with the microtubules to accelerate centrosome separation during mitosis...
  24. ncbi request reprint On the role of aurora-A in centrosome function
    Stéphanie Dutertre
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Université de Rennes I, IFR 97 Génomique Fonctionnelle et Santé, Faculte de Medecine, 2 Avenue du Pr Leon Bernard, CS 34317, 35043 Rennes Cedex, France
    Oncogene 21:6175-83. 2002
    ..Those transformed cells form tumours when implanted in immunodeficient mice, indicating that the kinase is an oncogene...
  25. ncbi request reprint Preparation and characterization of a human aurora-A kinase monoclonal antibody
    Jean Yves Cremet
    Groupe Cycle Cellulaire, UMR 6061 Génétique et Développement, CNRS Universite de Rennes 1, IFR 97 Génomique et Santé, Faculte de Medecine, Rennes Cedex, France
    Mol Cell Biochem 243:123-31. 2003
    ....
  26. ncbi request reprint Aurora/Ipl1p-related kinases, a new oncogenic family of mitotic serine-threonine kinases
    R Giet
    CNRS UPR41 Université de Rennes I, Groupe Cycle Cellulaire, Faculte de Medecine, CS 34317, France
    J Cell Sci 112:3591-601. 1999
    ..Because of the myriad of kinases identified, we suggest a generic name: Aurora/Ipl1p-related kinase (AIRK). We denote AIRKs with a species prefix and a number, e.g. HsAIRK1...
  27. ncbi request reprint Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter
    M Bernard
    Departement de Biologie et Genetique du Developpement, Groupe Cycle Cellulaire, CNRS UPR 41, Rennes Cedex, 35042, France
    Genomics 53:406-9. 1998
    ..The localization of STK13 on human chromosome 19 was confirmed by fluorescence in situ hybridization (FISH) using a genomic clone containing STK13 as a probe...
  28. ncbi request reprint [Control by centrosome of asymmetric repartition of cellular components]
    Régis Giet
    Med Sci (Paris) 19:656-8. 2003
  29. pmc Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules
    Régis Giet
    Department of Genetics, Cancer Research Campaign Cell Cycle Genetics Group, University of Cambridge, Cambridge CB2 3EH, UK
    J Cell Biol 156:437-51. 2002
    ....
  30. pmc APC/Fizzy-Related targets Aurora-A kinase for proteolysis
    Anna Castro
    Centre de Recherche de Biochimie Macromoleculaire, CNRS UPR 1086, 1919 Route de Mende, F 34293 Montpellier cedex 5, France
    EMBO Rep 3:457-62. 2002
    ..Aurora-A is thus the first protein, at least in our assay system, that undergoes a D-Box-dependent degradation mediated by APC/Fizzy-Related but not by APC/Fizzy...
  31. ncbi request reprint CDC25B phosphorylation by Aurora-A occurs at the G2/M transition and is inhibited by DNA damage
    Martine Cazales
    LBCMCP CNRS UMR5088 IFR109, Institut d Exploration Fonctionnelle des Génomes, Universite Paul Sabatier, Toulouse, France
    Cell Cycle 4:1233-8. 2005
    ..These results strongly suggest that Aurora-A-mediated phosphorylation of CDC25B at the centrosome is an important step contributing to the earliest events inducing mitosis, upstream of CDK1-cyclin B1 activation...
  32. pmc PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores
    Emilie Montembault
    Centre National de la Recherche Scientifique, Unité Mixte de Recherche 6061, Universite de Rennes I, Institut de Génétique et Développement, 35043 Rennes, France
    J Cell Biol 179:601-9. 2007
    ..Our data clearly identify PRP4 as a previously unrecognized kinetochore component that is necessary to establish a functional SAC...
  33. ncbi request reprint ASAP is a novel substrate of the oncogenic mitotic kinase Aurora-A: phosphorylation on Ser625 is essential to spindle formation and mitosis
    Magali Venoux
    Groupe Microtubules et Cycle Cellulaire, Institut de Genetique Humaine, CNRS UPR 1142, rue de la Cardonille, 34396 Montpellier Cedex 5, France
    Hum Mol Genet 17:215-24. 2008
    ..All together, these results suggest that we have identified a novel AurA substrate, pointing out ASAP as a new potential target for antitumoral drugs...
  34. ncbi request reprint Aurora B -TACC1 protein complex in cytokinesis
    Benedicte Delaval
    Department of Molecular Oncology, U119 INSERM, Institut Paoli Calmettes, IFR57, Marseille, France
    Oncogene 23:4516-22. 2004
    ..We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody - and consequently affects TACC1 localization at this site - and leads to abnormal cell division and multinucleated cells...
  35. pmc The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-A
    Anna Castro
    Centre de Recherche de Biochimie Macromoleculaire, CNRS UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
    EMBO Rep 3:1209-14. 2002
    ..The first one, which we designated D-Box-activating domain, within the N-terminal domain of Aurora-A confers the functionality to the second, a silent D-Box, present within the C-terminus of the kinase...
  36. ncbi request reprint Cdc2-cyclin B triggers H3 kinase activation of Aurora-A in Xenopus oocytes
    Gilliane Maton
    Laboratoire de Biologie du Developpement, Unite Mixte de Recherche CNRS 7622, Universite Pierre et Marie Curie, Boite 24, 4 place Jussieu, Paris 75252 Cedex 05, France
    J Biol Chem 278:21439-49. 2003
    ..Therefore, Aurora-A is under the control of Cdc2 in the Xenopus oocyte and could be involved in meiotic spindle establishment...
  37. ncbi request reprint TACC1-chTOG-Aurora A protein complex in breast cancer
    Nathalie Conte
    Department of Molecular Oncology, U119 INSERM, Institut Paoli Calmettes, IFR57, Marseille, France
    Oncogene 22:8102-16. 2003
    ....
  38. ncbi request reprint Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition
    Stéphanie Dutertre
    Groupe Cycle Cellulaire CNRS UMR6061 IFR97, Génomique Fonctionnelle et Santé, Universite de Rennes I, 35043 Rennes, France
    J Cell Sci 117:2523-31. 2004
    ..This phosphorylation might locally participate in the control of the onset of mitosis. These findings re-emphasise the role of the centrosome as a functional integrator of the pathways contributing to the triggering of mitosis...
  39. ncbi request reprint A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly
    Ming Ying Tsai
    Department of Embryology, Carnegie Institution of Washington Howard Hughes Medical Institute, Baltimore, MD 21210, USA
    Nat Cell Biol 5:242-8. 2003
    ....
  40. pmc Spindle pole regulation by a discrete Eg5-interacting domain in TPX2
    Frank Eckerdt
    Howard Hughes Medical Institute, Department of Pharmacology, University of Colorado School of Medicine, Aurora, Colorado 80045, USA
    Curr Biol 18:519-25. 2008
    ..Importantly, injection of Eg5 into TPX2-CT-arrested blastomeres causes resumption of cleavage. These results define a discrete domain within the C terminus of TPX2 that exerts a novel Eg5-dependent function in spindle pole segregation...
  41. pmc Ca2+ homeostasis regulates Xenopus oocyte maturation
    Lu Sun
    Department of Physiology and Biophysics, and the Arkansas Cancer Research Center, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
    Biol Reprod 78:726-35. 2008
    ..These findings argue that Ca2+ homeostasis is important in establishing the oocyte's competence to undergo maturation in preparation for fertilization and embryonic development...
  42. ncbi request reprint Oocyte maturation and cell cycle control: a farewell symposium for Pr Marcel Dorée
    Claude Prigent
    Biol Cell 96:181-5. 2004
    ....
  43. ncbi request reprint Expression of Aurora kinases in human thyroid carcinoma cell lines and tissues
    Salvatore Ulisse
    Department of Experimental Medicine and Pathology, University of Rome La Sapienza, Rome, Italy
    Int J Cancer 119:275-82. 2006
    ..These results suggest that the Aurora kinases may play a relevant role in malignant thyroid cancers, and may represent a putative therapeutic target for thyroid neoplasms...
  44. pmc Cdk1, Plks, Auroras, and Neks: the mitotic bodyguards
    Patrick Salaun
    Universite Rennes Fac de Medecine, Rennes, France
    Adv Exp Med Biol 617:41-56. 2008