Jean Marie Pages

Summary

Country: France

Publications

  1. pmc Efflux pump, the masked side of beta-lactam resistance in Klebsiella pneumoniae clinical isolates
    Jean Marie Pages
    UMR MD 1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, IFR88, Marseille, France
    PLoS ONE 4:e4817. 2009
  2. doi request reprint Mechanisms of drug efflux and strategies to combat them: challenging the efflux pump of Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
    Biochim Biophys Acta 1794:826-33. 2009
  3. pmc Antibacterial and antibiotic-potentiation activities of the methanol extract of some cameroonian spices against Gram-negative multi-drug resistant phenotypes
    Igor K Voukeng
    Department of Biochemistry, Faculty of Science, University of Dschang, P, O, Box 67, Dschang, Cameroon, Africa
    BMC Res Notes 5:299. 2012
  4. pmc Antibacterial activities of selected Cameroonian spices and their synergistic effects with antibiotics against multidrug-resistant phenotypes
    Aimé G Fankam
    Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon
    BMC Complement Altern Med 11:104. 2011
  5. ncbi request reprint An original deal for new molecule: reversal of efflux pump activity, a rational strategy to combat gram-negative resistant bacteria
    J M Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Curr Med Chem 18:2969-80. 2011
  6. ncbi request reprint Efflux pumps of gram-negative bacteria, a new target for new molecules
    Jean Marie Pages
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Curr Top Med Chem 10:1848-57. 2010
  7. doi request reprint The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultés de Médecine et de harmacie, Universite de la Mediterranee, Marseille, 13385, France
    Nat Rev Microbiol 6:893-903. 2008
  8. ncbi request reprint Production of the cryptic EefABC efflux pump in Enterobacter aerogenes chloramphenicol-resistant mutants
    Muriel Masi
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Université de la Méditerranée 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    J Antimicrob Chemother 57:1223-6. 2006
  9. pmc Omp35, a new Enterobacter aerogenes porin involved in selective susceptibility to cephalosporins
    Charleric Bornet
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 48:2153-8. 2004
  10. pmc mar Operon involved in multidrug resistance of Enterobacter aerogenes
    Renaud Chollet
    Enveloppe Bacterienne, Antibiotiques et Colonisation, CJF 9606 EA 2197, Facultés de Médecine et Pharmacie, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 46:1093-7. 2002

Collaborators

Detail Information

Publications64

  1. pmc Efflux pump, the masked side of beta-lactam resistance in Klebsiella pneumoniae clinical isolates
    Jean Marie Pages
    UMR MD 1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, IFR88, Marseille, France
    PLoS ONE 4:e4817. 2009
    ..pneumoniae isolates that are non susceptible to cefoxitin (FOX) and susceptible to amoxicillin+clavulanic acid in our hospital. Assessing the role of efflux pumps in this beta-lactam phenotype was the aim of this study...
  2. doi request reprint Mechanisms of drug efflux and strategies to combat them: challenging the efflux pump of Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
    Biochim Biophys Acta 1794:826-33. 2009
    ....
  3. pmc Antibacterial and antibiotic-potentiation activities of the methanol extract of some cameroonian spices against Gram-negative multi-drug resistant phenotypes
    Igor K Voukeng
    Department of Biochemistry, Faculty of Science, University of Dschang, P, O, Box 67, Dschang, Cameroon, Africa
    BMC Res Notes 5:299. 2012
    ....
  4. pmc Antibacterial activities of selected Cameroonian spices and their synergistic effects with antibiotics against multidrug-resistant phenotypes
    Aimé G Fankam
    Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon
    BMC Complement Altern Med 11:104. 2011
    ..The present study was designed to evaluate the antibacterial activities of the methanol extracts of eleven Cameroonian spices on a panel of twenty nine Gram negative bacteria including MDR strains...
  5. ncbi request reprint An original deal for new molecule: reversal of efflux pump activity, a rational strategy to combat gram-negative resistant bacteria
    J M Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Curr Med Chem 18:2969-80. 2011
    ..This new family of molecules represents the first antibacterial class of compound specifically targeting active transport in the bacterial cell...
  6. ncbi request reprint Efflux pumps of gram-negative bacteria, a new target for new molecules
    Jean Marie Pages
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Curr Top Med Chem 10:1848-57. 2010
    ....
  7. doi request reprint The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria
    Jean Marie Pages
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultés de Médecine et de harmacie, Universite de la Mediterranee, Marseille, 13385, France
    Nat Rev Microbiol 6:893-903. 2008
    ....
  8. ncbi request reprint Production of the cryptic EefABC efflux pump in Enterobacter aerogenes chloramphenicol-resistant mutants
    Muriel Masi
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Université de la Méditerranée 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    J Antimicrob Chemother 57:1223-6. 2006
    ..AcrAB-TolC is the major tripartite multidrug efflux pump in Enterobacter aerogenes while EefABC is a cryptic efflux system. This study was conducted to identify and characterize E. aerogenes mutants producing the EefABC efflux pump...
  9. pmc Omp35, a new Enterobacter aerogenes porin involved in selective susceptibility to cephalosporins
    Charleric Bornet
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 48:2153-8. 2004
    ....
  10. pmc mar Operon involved in multidrug resistance of Enterobacter aerogenes
    Renaud Chollet
    Enveloppe Bacterienne, Antibiotiques et Colonisation, CJF 9606 EA 2197, Facultés de Médecine et Pharmacie, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 46:1093-7. 2002
    ..aerogenes MarA induces a multidrug resistance phenotype in a susceptible strain, demonstrated by a noticeable resistance to various antibiotics, a decrease in immunodetected porins, and active efflux of norfloxacin...
  11. pmc An early response to environmental stress involves regulation of OmpX and OmpF, two enterobacterial outer membrane pore-forming proteins
    Myrielle Dupont
    UMR MD1, Faculte de Medecine, 27 Blvd Jean Moulin, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 51:3190-8. 2007
    ..Consequently, the early control of ompX and ompF induced by external stresses may represent a preliminary response to antibiotics, thus triggering the initial bacterial line of defense against antibiotherapy...
  12. pmc Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region
    Jérôme Bredin
    CJF9606 EA2197, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem J 363:521-8. 2002
    ..These results suggest that the Lys-16 residue plays an important role in the process of diffusion through the OmpF lumen...
  13. ncbi request reprint Identification of an OprD homologue in Acinetobacter baumannii
    Myrielle Dupont
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, Marseille Cedex 05, France
    J Proteome Res 4:2386-90. 2005
    ..By electrophoresis and MALDI-MS analyses, the 43 kDa OMP was identified as a protein belonging to the OprD family, a basic amino acid and imipenem porin...
  14. ncbi request reprint Chloroquinolines block antibiotic efflux pumps in antibiotic-resistant Enterobacter aerogenes isolates
    Didier Ghisalberti
    EA2197, IFR48, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Int J Antimicrob Agents 27:565-9. 2006
    ..Some of the molecules tested in this work are able to inhibit the main efflux pump (AcrAB-TolC), which is involved in E. aerogenes antibiotic resistance...
  15. ncbi request reprint Membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens
    Anne Davin-Regli
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Curr Drug Targets 9:750-9. 2008
    ..These effectors activate the expression of specific global regulators, marA, ramA, or target other genes located downstream in the regulation cascade...
  16. pmc Modification of outer membrane protein profile and evidence suggesting an active drug pump in Enterobacter aerogenes clinical strains
    Stéphane Gayet
    EA2197 IFR 48, Faculte de Medecine, 13385 Marseille Cedex 05 UMR 5048, CNRS, U554 INSERM, 34090 Montpellier, France
    Antimicrob Agents Chemother 47:1555-9. 2003
    ..The resistance strategies used by these isolates appear to be similar to that induced by stress in Escherichia coli cells...
  17. ncbi request reprint Enterobacter aerogenes OmpX, a cation-selective channel mar- and osmo-regulated
    Myrielle Dupont
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
    FEBS Lett 569:27-30. 2004
    ..aerogenes ATCC 13048 strain. In addition, OmpX synthesis increased under conditions in which the expression of the E. aerogenes major non-specific porins, Omp36 and Omp35, decreased...
  18. pmc Inhibitors of antibiotic efflux in resistant Enterobacter aerogenes and Klebsiella pneumoniae strains
    Jacqueline Chevalier
    EA2197, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 48:1043-6. 2004
    ..aerogenes and K. pneumoniae isolates...
  19. pmc Colicins, spermine and cephalosporins: a competitive interaction with the OmpF eyelet
    Jérôme Bredin
    EA2197, IFR48, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem J 376:245-52. 2003
    ..D113 appeared to play an essential role for colicin A activity, whereas colicin N activity was more dependent on D121 than on D113...
  20. pmc The AcrAB-TolC efflux pump contributes to multidrug resistance in the nosocomial pathogen Enterobacter aerogenes
    Elizabeth Pradel
    INSERM CJF9606 EA2197, IFR48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 46:2640-3. 2002
    ..Both derivatives were more susceptible to antibiotics than the parental strain. Sequence analysis and complementation experiments revealed that the multidrug-resistant isolate is an acrR mutant...
  21. pmc Alkylaminoquinolines inhibit the bacterial antibiotic efflux pump in multidrug-resistant clinical isolates
    Monique Malléa
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem J 376:801-5. 2003
    ..Inhibitory activity of this alkylaminoquinoline is observed on clinical isolates showing different resistance phenotypes...
  22. ncbi request reprint Imipenem and expression of multidrug efflux pump in Enterobacter aerogenes
    Charleric Bornet
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 05, Marseille Cedex, France
    Biochem Biophys Res Commun 301:985-90. 2003
    ..The overexpression of marA, involved in the genetic control of membrane permeability via porin and efflux pump expression, indicated the activation of the resistance genetic cascade in imipenem resistant variants...
  23. pmc The AcrAB-TolC pump is involved in macrolide resistance but not in telithromycin efflux in Enterobacter aerogenes and Escherichia coli
    Renaud Chollet
    Enveloppe Bacterienne, Perméabilité et Antibiotique, Faculte de Medecine, Universite de la Mediterranee, Marseille, France
    Antimicrob Agents Chemother 48:3621-4. 2004
    ..aerogenes acrAB or tolC derivatives compared to that in the parental strain. Two independent efflux pumps, inhibited by phenylalanine arginine beta-naphthylamide, expel macrolides and telithromycin in E. aerogenes...
  24. doi request reprint An alkylaminoquinazoline restores antibiotic activity in Gram-negative resistant isolates
    Abdallah Mahamoud
    UMR MD 1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Microbiology 157:566-71. 2011
    ..A molecule exhibiting a morpholine functional group and a propyl extension of the side chain was more active...
  25. ncbi request reprint Quinoline derivatives as promising inhibitors of antibiotic efflux pump in multidrug resistant Enterobacter aerogenes isolates
    Abdallah Mahamoud
    EA2197, IFR48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France
    Curr Drug Targets 7:843-7. 2006
    ..Here, we discuss the respective properties of this molecular family, taking into account the recent insights into the structural data of AcrB pump...
  26. pmc Identification and evolution of drug efflux pump in clinical Enterobacter aerogenes strains isolated in 1995 and 2003
    Jacqueline Chevalier
    UMR MD1, IFR 48, Facultes de Medecine et de Pharmacie, Marseille, France
    PLoS ONE 3:e3203. 2008
    ....
  27. pmc Use of the omp50 gene for identification of Campylobacter species by PCR
    Luc Dedieu
    EA 2197 and IFR 48, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 5, France
    J Clin Microbiol 42:2301-5. 2004
    ..lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species...
  28. ncbi request reprint Molecular basis of macrolide resistance in Campylobacter: role of efflux pumps and target mutations
    Laurent Mamelli
    Universite de la Mediterranee, Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, 27 Bd Jean Moulin 13385, Marseille Cedex 05, France
    J Antimicrob Chemother 56:491-7. 2005
    ..Erythromycin is the drug of choice to treat human campylobacteriosis. Campylobacter isolates exhibit two different phenotypes with regard to erythromycin resistance: high-level resistant strains (HLR) and low-level resistant strains (LLR)...
  29. pmc The eefABC multidrug efflux pump operon is repressed by H-NS in Enterobacter aerogenes
    Muriel Masi
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, Faculte de Medecine, Marseille, France
    J Bacteriol 187:3894-7. 2005
    ..However, increased transcription from Peef was observed in an E. coli hns mutant. In addition, EefA was detected in E. aerogenes expressing a dominant negative E. coli hns allele...
  30. ncbi request reprint Chloramphenicol and expression of multidrug efflux pump in Enterobacter aerogenes
    Didier Ghisalberti
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem Biophys Res Commun 328:1113-8. 2005
    ..MDR in the chloramphenicol-resistant variant is linked to the overexpression of the major AcrAB-TolC efflux system. This overexpression seems unrelated to the global Mar and the local AcrR regulatory pathways...
  31. doi request reprint Quinazoline derivatives are efficient chemosensitizers of antibiotic activity in Enterobacter aerogenes, Klebsiella pneumoniae and Pseudomonas aeruginosa resistant strains
    Jacqueline Chevalier
    UMR MD1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, IFR88, Marseille, France
    Int J Antimicrob Agents 36:164-8. 2010
    ..Quinazoline molecules exhibiting a nitro functional group are more active, and structure-activity relationship studies may be undertaken to identify the pharmacophoric group involved in the AcrB and MexB affinity sites...
  32. ncbi request reprint Expression and purification of native and truncated forms of CadF, an outer membrane protein of Campylobacter
    Laurent Mamelli
    EA 2197, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    Int J Biol Macromol 39:135-40. 2006
    ..5 microg/mg of bacterial dry weight) and of the native CadF protein (3.5 microg/mg of bacterial dry weight) for further studies...
  33. pmc Environmental regulation of Campylobacter jejuni major outer membrane protein porin expression in Escherichia coli monitored by using green fluorescent protein
    Luc Dedieu
    CJF 96 06 INSERM, EA 2197 and IFR 48, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 5, France
    Appl Environ Microbiol 68:4209-15. 2002
    ..These findings are a step towards determining the role of outer membrane proteins in the adaptation of C. jejuni to its environment...
  34. ncbi request reprint Resistance to imipenem, cefepime, and cefpirome associated with mutation in Omp36 osmoporin of Enterobacter aerogenes
    Aurélie Thiolas
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem Biophys Res Commun 317:851-6. 2004
    ..Microbiol. 41 (2001) 189]. This is the first genetic identification of impermeability-mediated resistance to beta-lactams in various clinical E. aerogenes strains...
  35. pmc Successive emergence of Enterobacter aerogenes strains resistant to imipenem and colistin in a patient
    Aurélie Thiolas
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, IFR48, Faculte de Medecine, 27 Blvd Jean Moulin, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 49:1354-8. 2005
    ..The involved resistance mechanisms exhibited by the successive isolates were associated with alterations of the outer membrane that caused a porin decrease and lipopolysaccharide modifications...
  36. doi request reprint An adaptive response of Enterobacter aerogenes to imipenem: regulation of porin balance in clinical isolates
    Jean Philippe Lavigne
    UMR MD1, Aix Marseille Universite, IRBA, Facultes de Medecine et de Pharmacie, Marseille, France
    Int J Antimicrob Agents 41:130-6. 2013
    ..This study indicates that the alteration in porin expression, including the shift of porin expression and lack of porins, contribute to the E. aerogenes adaptive response to IPM treatment...
  37. pmc Occurrence of efflux mechanism and cephalosporinase variant in a population of Enterobacter aerogenes and Klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases
    Que Tien Tran
    Facultés de Médecine et Pharmacie, Transporteurs Membranaires, Chimioresistance et Drug Design, UMR MD1, Universite de la Mediterranee, Marseille, France
    Antimicrob Agents Chemother 53:1652-6. 2009
    ..AcrA-TolC overproduction in E. aerogenes isolates was noted. An analysis of four E. aerogenes isolates for which cefepime MICs were high revealed no modification in porin expression but a new specific mutation in the AmpC beta-lactamase...
  38. pmc How beta-lactam antibiotics enter bacteria: a dialogue with the porins
    Chloe E James
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, IFR 88, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e5453. 2009
    ..Clinical reports have linked the MDR phenotype to altered membrane permeability including porin modification and efflux pump expression...
  39. ncbi request reprint A phenylalanine-arginine beta-naphthylamide sensitive multidrug efflux pump involved in intrinsic and acquired resistance of Campylobacter to macrolides
    Laurent Mamelli
    EA 2197, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Int J Antimicrob Agents 22:237-41. 2003
    ..To conclude, besides mutations, erythromycin resistance in Campylobacter may also be due to an efflux mechanism sensitive to PAbetaN...
  40. ncbi request reprint Chromosomal His-tagging: an alternative approach to membrane protein purification
    Laurent Mamelli
    UMR MD1, IFR 48, Faculte de Medecine, Universite de la Mediterranee, Marseille Cedex, France
    Proteomics 7:399-402. 2007
    ..CadF, an E. coli OmpA-like protein, facilitates the binding of C. jejuni to the extracellular matrix component, fibronectin...
  41. ncbi request reprint Beta-lactam screening by specific residues of the OmpF eyelet
    Stephanie Vidal
    GERCTOP UMR6178 CNRS, IFR48, Faculte de Pharmacie, Universite de la Mediterranee, 13385 Marseille Cedex 05, France
    J Med Chem 48:1395-400. 2005
    ..Molecular modeling suggests that the charges harbored by the beta-lactam molecules interact with the charged residues located inside the porin eyelet...
  42. doi request reprint Essential oils from Moroccan plants as potential chemosensitisers restoring antibiotic activity in resistant Gram-negative bacteria
    Mariam Fadli
    UMR MD1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Int J Antimicrob Agents 38:325-30. 2011
    ..These results demonstrate that these essential oils can alter efflux pump activity and may be attractive candidates to develop new drugs for chemosensitising multidrug-resistant strains to clinically used antibiotics...
  43. ncbi request reprint Overexpression and purification of the three components of the Enterobacter aerogenes AcrA-AcrB-TolC multidrug efflux pump
    Muriel Masi
    EA2197, INSERM CJF9606, Enveloppe Bacterienne, Perméabilité et Antibiotiques, Faculte de Medecine Timone, 27 Bd Jean Moulin, 13385 cedex 05, Marseille, France
    J Chromatogr B Analyt Technol Biomed Life Sci 786:197-205. 2003
    ..After ultracentrifugation, the pellet was treated with 5% Triton X-100, 5 mM EDTA to solubilize the outer membrane proteins. Approximately 5 mg of 95% pure TolC-6His trimers per liter of culture was purified by INAC...
  44. ncbi request reprint Computer simulation of spermine-porin channel interactions
    Stephanie Vidal
    GERCTOP, UMR CNRS 6009, Universite de la Mediterranee, 13385 Marseille, France
    In Vivo 16:111-6. 2002
    ..This interaction introduces several changes in the internal morphology of the channel which are responsible for the inhibition of antibiotic uptake using the porin route...
  45. pmc Geraniol restores antibiotic activities against multidrug-resistant isolates from gram-negative species
    Vannina Lorenzi
    Universite de la Mediterranee, UMR MD1, IFR88, Faculte de Medecine, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 53:2209-11. 2009
    ..Geraniol, a component of one fraction, significantly increased the efficacy of beta-lactams, quinolones, and chloramphenicol...
  46. ncbi request reprint Antibiotic efflux pumps in Gram-negative bacteria: the inhibitor response strategy
    Abdallah Mahamoud
    UMR MD 1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, F 13385 Marseille cedex 05, France
    J Antimicrob Chemother 59:1223-9. 2007
    ....
  47. doi request reprint The omp50 gene is transcriptionally controlled by a temperature-dependent mechanism conserved among thermophilic Campylobacter species
    Luc Dedieu
    UMR MD 1, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, Marseille Cedex 5, France
    Res Microbiol 159:270-8. 2008
    ..coli. The same regulation was also observed in wild-type C. lari strains and in a C. coli strain supplemented by the plasmid, suggesting that omp50 expression is controlled by a mechanism conserved among Campylobacter species...
  48. ncbi request reprint Chemosensitizers in drug transport mechanisms involved in protozoan resistance
    Bruno Pradines
    Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Medecine Tropicale du Service de Sante des Armees, Marseille, France
    Curr Drug Targets Infect Disord 5:411-31. 2005
    ..Here, are summarized the molecular bases of antiparasitic resistance emphasizing recent developments with compounds acting on trans-membrane proteins involved in drug efflux or uptake...
  49. pmc Functional refolding of the Campylobacter jejuni MOMP (major outer membrane protein) porin by GroEL from the same species
    Florence Goulhen
    EA 2197, IFR 48, Faculte de Medecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem J 378:851-6. 2004
    ..Under these conditions, 95% of denatured MOMP was refolded after a 15 min incubation. This approach represents an alternative method in studies of membrane protein refolding...
  50. ncbi request reprint [Bacterial porin and antibiotic susceptibility]
    Jean Marie Pages
    EA2197, Enveloppe Bacterienne, Perméabilité et Antibiotiques, 27, Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Med Sci (Paris) 20:346-51. 2004
    ..All these aspects play a key role in both membrane permeability and efficiency of the antibiotic resistance process...
  51. pmc RamA is an alternate activator of the multidrug resistance cascade in Enterobacter aerogenes
    Renaud Chollet
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA 2197, IFR 48, Facultés de Médecine et Pharmacie, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Antimicrob Agents Chemother 48:2518-23. 2004
    ..We demonstrate here that RamA is a transcriptional activator of the Mar regulon and is also a self-governing activator of the MDR cascade...
  52. ncbi request reprint Inhibitors of efflux pumps in Gram-negative bacteria
    Jean Marie Pages
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille, France
    Trends Mol Med 11:382-9. 2005
    ..Current progress for the clinical use of efflux-pump inhibitors and new strategies to combat the drug-efflux mechanisms will be discussed...
  53. ncbi request reprint Inhibitors of antibiotic efflux pump in resistant Enterobacter aerogenes strains
    Monique Malléa
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Biochem Biophys Res Commun 293:1370-3. 2002
    ....
  54. doi request reprint Polyamino geranic derivatives as new chemosensitizers to combat antibiotic resistant gram-negative bacteria
    Jean Michel Brunel
    Centre de Recherche en Cancérologie CRCM, CNRS, UMR7258, Marseille, France
    Bioorg Med Chem 21:1174-9. 2013
    ..These compounds induced a significant decrease of antibiotic resistance in two Gram-negative bacterial MDR strains. Our data suggested that their mechanism of action is closely associated with the inhibition of the efflux pumps...
  55. doi request reprint New peptide deformylase inhibitors and cooperative interaction: a combination to improve antibacterial activity
    Emilie Goemaere
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Aix Marseille Universite, IRBA, Marseille, France
    J Antimicrob Chemother 67:1392-400. 2012
    ....
  56. pmc Squalamine: an appropriate strategy against the emergence of multidrug resistant gram-negative bacteria?
    Chanaz Salmi
    UMR MD1, Facultes de Medecine et de Pharmacie, Marseille, France
    PLoS ONE 3:e2765. 2008
    ....
  57. doi request reprint Strategies for bypassing the membrane barrier in multidrug resistant Gram-negative bacteria
    Jean Michel Bolla
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    FEBS Lett 585:1682-90. 2011
    ..Different strategies could be proposed to bypass the bacterial membrane barrier, comprising influx and efflux mechanisms, in order to restore the activity of antibiotics against resistant bacteria...
  58. ncbi request reprint The Enterobacter aerogenes outer membrane efflux proteins TolC and EefC have different channel properties
    Muriel Masi
    UMR MD 1, IFR48, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, Marseille, France
    Biochim Biophys Acta 1768:2559-67. 2007
    ..Another characteristic of the TolC and EefC channels was the systematic closure induced by acidic pH. These results are discussed in respect to the physiological functions and structural models of TolC and EefC...
  59. ncbi request reprint Propyl paraben induces potassium efflux in Escherichia coli
    Jérôme Bredin
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, IFR 48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    J Antimicrob Chemother 55:1013-5. 2005
    ..Parabens are currently used as antibacterial preservatives in pharmaceutical, cosmetic and food products but there are no precise data concerning their activity on bacterial membranes...
  60. pmc Involvement of the efflux pumps in chloramphenicol selected strains of Burkholderia thailandensis: proteomic and mechanistic evidence
    Fabrice V Biot
    UMR MD 1, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, IFR88, Marseille, France
    PLoS ONE 6:e16892. 2011
    ..Antibiotics known to easily induce overexpression of these efflux pumps should be used with discernment in the treatment of Burkholderia infections...
  61. ncbi request reprint Efflux mechanism, an attractive target to combat multidrug resistant Plasmodium falciparum and Pseudomonas aeruginosa
    Sandrine Alibert-Franco
    UMR MD 1, Transporteurs Membranaires, Chimioresistance et Drug Design, Faculte de Medecine, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France
    Curr Med Chem 16:301-17. 2009
    ..The efflux mechanisms and the development of efflux pump inhibitors (EPIs) are discussed regarding these two pathogens...
  62. pmc New antibiotic molecules: bypassing the membrane barrier of gram negative bacteria increases the activity of peptide deformylase inhibitors
    Laurent Mamelli
    UMR MD1, Transporteurs Membranaires, Chimioresistance et Drug Design, Facultes de Medecine et de Pharmacie, IFR 88, Universite de la Mediterranee, Marseille, France
    PLoS ONE 4:e6443. 2009
    ..The bacterial susceptibility to such a cytoplasmic target primarily depends on the capacity of the compound to reach and accumulate in the cytosol...
  63. ncbi request reprint Thanatin activity on multidrug resistant clinical isolates of Enterobacter aerogenes and Klebsiella pneumoniae
    Jean Marie Pages
    EA2197, IFR48, Faculte de Medecine, Universite de la Mediterranee, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France
    Int J Antimicrob Agents 22:265-9. 2003
    ..In addition, thanatin was able to improve the activity of structurally unrelated antibiotics (norfloxacin, chloramphenicol, tetracycline) on a multidrug- resistant E. aerogenes clinical isolate...
  64. ncbi request reprint Crystallization and preliminary crystallographic studies of MOMP (major outer membrane protein) from Campylobacter jejuni
    Jean Michel Bolla
    Enveloppe Bacterienne, Perméabilité et Antibiotiques, EA2197, Faculte de Medecine, Universite de la Mediterranee, Boulevard Jean Moulin, 13385 Marseille, France
    Acta Crystallogr D Biol Crystallogr 60:2349-51. 2004
    ..4 A Da(-1)). The other form exhibits trigonal symmetry (space group R3) with hexagonal unit-cell parameters a = b = 94.2, c = 161.2 A, but diffracts X-rays poorly to about 4 A with significant anisotropy...