- Photogenotoxicity of mammalian cells: a review of the different assays for in vitro testingJean Roch Meunier
L OREAL Advanced Research, Life Sciences Research, Aulnay sous Bois, France
Photochem Photobiol 75:437-47. 2002..Ultimately, it can lead to the induction of hereditary DNA modification. A variety of assays are proposed to specifically address all these particular consequences of UV-induced toxicity...
- Importance of UVA photoprotection as shown by genotoxic related endpoints: DNA damage and p53 statusLaurent Marrot
L OREAL Research, Life Sciences Research, Phototoxicology Unit, Genotoxicity Group 1, av E Schueller, 93600 Aulnay sous Bois, France
Mutat Res 571:175-84. 2005..These data demonstrate that the loss of absorbing efficiency within UVA wavelengths due to photounstability may have detrimental consequences leading to impairments implicated in genotoxic events...
- TRP-2 specifically decreases WM35 cell sensitivity to oxidative stressQuentin Michard
L Oreal Recherche, 90 rue du General Roguet, 92583 Clichy Cedex, France
Free Radic Biol Med 44:1023-31. 2008..Nevertheless, these properties appeared to depend on a particular cell environment because expression of TRP-2 failed to rescue HEK epithelial cells exposed to similar treatments...
- Comet assay combined with p53 detection as a sensitive approach for DNA photoprotection assessment in vitroLaurent Marrot
Genotoxicity Group, L OREAL Research, 1 Avenue E Schueller, 96300 Aulnay sous Bois, France
Exp Dermatol 11:33-6. 2002..As a consequence, such a formulation was not as protective as a photostable one at the genomic level...
- The significance of Nrf2 pathway in (photo)-oxidative stress response in melanocytes and keratinocytes of the human epidermisLaurent Marrot
Department of Safety Research, Phototoxicity Unit, L OREAL Research, Aulnay sous Bois, France
Pigment Cell Melanoma Res 21:79-88. 2008..The results hereby presented should contribute to a better understanding of the molecular mechanisms involved in skin adaptation to environmental stress...
- Molecular responses to photogenotoxic stress induced by the antibiotic lomefloxacin in human skin cells: from DNA damage to apoptosisLaurent Marrot
Genotoxicity Group, L OREAL Research, 1 avenue Eugene Schueller, 93600 Aulnay sous Bois, France
J Invest Dermatol 121:596-606. 2003..These data show that the phototoxicity of a given drug can be driven by different mechanisms and that its biologic impact varies according to cell type...
- Molecular responses to stress induced in normal human caucasian melanocytes in culture by exposure to simulated solar UVLaurent Marrot
L Oreal Recherche, Aulnay sous Bois, France
Photochem Photobiol 81:367-75. 2005..They provide new data related to gene expression and suggest that melanin in light skin could contribute to sunlight-induced genotoxicity and maybe to melanocyte transformation...
- Skin DNA photodamage and its biological consequencesLaurent Marrot
L OREAL Advanced Research, Life Science Research, Investigative Toxicology Department, Genotoxicity Group, Aulnay sous Bois, France
J Am Acad Dermatol 58:S139-48. 2008..Besides the biological consequences of DNA photolesions, this article also deals with technologies used for their detection and shows how helpful such approaches can be to assess photoprotection provided by sunscreens...
- The EpiSkin phototoxicity assay (EPA): development of an in vitro tiered strategy using 17 reference chemicals to predict phototoxic potencyDamien Lelièvre
L OREAL Research, Life Sciences, 1 avenue Eugene Schueller, 93 600 Aulnay Sous Bois, France
Toxicol In Vitro 21:977-95. 2007..1%. The performances of the EPA showed that the EpiSkin(LM) model is an interesting tool able to integrate decision-making processes to address the question of phototoxicity linked to the application site...
- Development of genotoxicity test procedures with Episkin, a reconstructed human skin model: towards new tools for in vitro risk assessment of dermally applied compounds?Nicole Flamand
L Oreal, Advanced Research, Life Sciences, Safety Research Department, Phototoxicity Genotoxicity Carcinogenicity Group, 1, avenue E Schueller, F 93600 Aulnay Sous Bois, France
Mutat Res 606:39-51. 2006..This second way of using human reconstructed skin for genotoxicity testing aims at improving the relevance of exposure conditions in in vitro genotoxicity assays for dermally applied compounds...
- Increased melanogenesis is a risk factor for oxidative DNA damage--study on cultured melanocytes and atypical nevus cellsNico P M Smit
Department of Clinical Chemistry, Leiden University Medical Center, Leiden, The Netherlands
Photochem Photobiol 84:550-5. 2008....