Research Topics
| C A MarquetteSummaryCountry: France Publications
| Collaborators
|
Detail Information
Publications
Design of luminescent biochips based on enzyme, antibody, or DNA composite layersChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5013 CNRS, Université Claude Bernard Lyon 1 Bât CPE, 43 Bd 11 Nov 1918, 69622, Villeurbanne Cedex, France
Anal Bioanal Chem 377:922-8. 2003..In a similar way, the use of oligonucleotide-immobilised beads enabled the realisation of DNA-sensitive biochips which could be used to detect a biotin-labelled sequence al a level of 5x10(8) molecules...- State of the art and recent advances in immunoanalytical systemsChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5013, CNRS Universite Claude Bernard Lyon 1, Bat CPE, Villeurbanne, France
Biosens Bioelectron 21:1424-33. 2006..Homogeneous immunosensors, heterogeneous immunosensors, integrated immunosensors and biochip format immunosensors are presented, based on optical, electrochemical, magnetic or mechanical detection/transduction systems... - DNA covalent immobilization onto screen-printed electrode networks for direct label-free hybridization detection of p53 sequencesC A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR CNRS 5013 Bat CPE Université Claude Bernard Lyon 1, Villeurbanne, France
Anal Chem 78:959-64. 2006..Discrimination between complementary, noncomplementary, and single-nucleotide mismatch targets was easily accomplished... - Electro-chemiluminescent biosensingChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Villeurbanne, France
Anal Bioanal Chem 390:155-68. 2008..The mechanism, principle, and experimental conditions of these two reactions are described. Then, for each category of analytical tools, experimental set-ups and performances are presented and discussed... 
Microfluidic biochip for chemiluminescent detection of allergen-specific antibodiesKevin A Heyries
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Lyon 1, CNRS 5246 ICBMS, Bat CPE, Villeurbanne Cedex, France
Biosens Bioelectron 23:1812-8. 2008..Finally, sera from allergic patients were assayed using the microfluidic device modified with apple hazelnut and pollen allergen. The results obtained compared favourably with those obtained with the classical Pharmacia CAP system...- Recent advances in multiplex immunoassaysChristophe A Marquette
Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires GEMBAS, Universite Lyon 1 CNRS 5246 ICBMS, Batiment CPE 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Bioanalysis 4:927-36. 2012..Finally, the degree of integration in a complete system, incorporating fluid handling and detection was also taken into account... - Applications of the luminol chemiluminescent reaction in analytical chemistryChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5013 EMB2, CNRS Universite Claude Bernard Lyon 1, Bat CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Anal Bioanal Chem 385:546-54. 2006..Chemiluminescent and electrochemiluminescent reactions were also found to merge in biochip and microarray development as a possible substitute to the well-established but hardly quantitative fluorescent detections... - Electroaddressed immobilization of recombinant HIV-1 P24 capsid protein onto screen-printed arrays for serological testingChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, EMB2 UMR 5013 CNRS Université Lyon 1, Batiment CPE 43, bd du 11 novembre 1918 69622 Villeurbanne, Cedex, France
Anal Biochem 340:14-23. 2005..The biochip was used to perform an HIV-1 serological test in human sera. HIV-1 seropositive and seronegative sera were easily discriminated using serum dilutions greater than 1/10,000... - Latex bead immobilisation in PDMS matrix for the detection of p53 gene point mutation and anti-HIV-1 capsid protein antibodiesChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, EMB2 UMR 5013 CNRS, Universite Lyon 1, Batiment CPE 43, Bd du 11 Novembre, 1918 69622, Villeurbanne, France
Anal Bioanal Chem 381:1019-24. 2005..The positivity of the sera was easily discriminated at serum dilutions below 1:1,000... - Conducting elastomer surface texturing: a path to electrode spotting. Application to the biochip productionChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Claude Bernard Lyon 1, UMR 5013 EMB2 CNRS, Bat CPE, Building 43, 11 November 1918, Villeurbanne 69622, Cedex, France
Biosens Bioelectron 20:197-203. 2004..Detection limits of 10 microM for lactate and choline and 20 microM for glucose were found, with detection ranging over two decades at least... - Disposable screen-printed chemiluminescent biochips for the simultaneous determination of four point-of-care relevant proteinsC A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Lyon 1 CNRS 5246 ICBMS, Bat CPE, 43 Bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France
Anal Bioanal Chem 393:1191-8. 2009..The four different assays were also validated in spiked 40-times-diluted human sera, using LowCross buffer, and were shown to work simultaneously in this complex medium... - "Macromolecules to PDMS transfer" as a general route for PDMS biochipsKevin A Heyries
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Lyon 1, CNRS 5246 ICBMS, Bat CPE 43, Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
Biosens Bioelectron 24:1146-52. 2009..The successful attachment of HeLa and BALB/3T3 cells was demonstrated with optical microscopy and specific staining of actin and vinculin... - Chemiluminescent choline biosensor using histidine-modified peroxidase immobilised on metal-chelate substituted beads and choline oxidase immobilised on anion-exchanger beads co-entrapped in a photocrosslinkable polymerV C Tsafack
, UPRESA CNRS 5013, , Villeurbanne, France
Biosens Bioelectron 15:125-33. 2000..5 pmol and the working range was 0.35 pmol-10 nmol. Moreover, the cycle time was only 2.5 min with the new sensing layer, and a long operational stability of 150 successive assays was found, with a variation coefficient of 2.6%... - Chelating Langmuir-Blodgett film: a new versatile chemiluminescent sensing layer for biosensor applicationsAurélie A M Santafé
Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, CNRS, CPE Lyon, INSA de Lyon, UMR 5246, Universite Lyon 1, Villeurbanne, F 69622, Lyon, France
Langmuir 26:2160-6. 2010..This signal variation was shown to be directly correlated to the histamine concentration with a limit of detection of 2 microg/mL... - Straightforward protein immobilization on Sylgard 184 PDMS microarray surfaceKevin A Heyries
Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Bâtiment CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Langmuir 23:4523-7. 2007..The proteins were shown to be easily recognized by their specific antibodies, giving antibody detection limits in the fmol range... - Biochips: non-conventional strategies for biosensing elements immobilizationChristophe A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5246 ICBMS, CNRS Universite Claude Bernard Lyon 1, Bat CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Front Biosci 13:382-400. 2008..Finally, bead-assisted biomolecules immobilization will be presented as an open field of research for biochip developments... - Multiplexed immunoassay for the rapid detection of anti-tumor-associated antigens antibodiesC Desmet
Equipe Génie Enzymatique, Membranes Biomimetiques et Assemblages Supramoleculaires, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Villeurbanne Cedex, France
Analyst 136:2918-24. 2011..This protein subpanel was proven to be able to detect 72.2% of the cancer patients tested (within a 34 panel of 18 patients and 16 healthy donors)... - Straightforward protein immobilization using redox-initiated poly(methyl methacrylate) polymerizationKevin A Heyries
Laboratoire de Genie Enzymatique et Biomoleculaire, Universite Lyon 1 CNRS 5246 ICBMS, Bat CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Langmuir 25:661-4. 2009.... - A versatile method for direct and covalent immobilization of DNA and proteins on biochipsBenjamin P Corgier
, UMR 5246, , , Villeurbanne, France
Angew Chem Int Ed Engl 46:4108-10. 2007 - 1-Ethyl-3-methylimidazolium ethylsulfate/copper catalyst for the enhancement of glucose chemiluminescent detection: effects on light emission and enzyme activityAurélie A M Santafé
Laboratoire de Genie Enzymatique, Membranes Biomimetiques et Assemblages Supramoleculaires, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Batiment CPE 43, Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
Anal Chem 82:2401-4. 2010..Interesting effects of [Emim][EtSO(4)] on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 microM) at pH 8.0... - "Print-n-Shrink" technology for the rapid production of microfluidic chips and protein microarraysKevin Sollier
Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moléculaire et Supramoléculaire, Université Lyon1 CNRS 5246 ICBMS, Bat CPE 43 Bd du 11 Nov, 69622 Villeurbanne, France
Lab Chip 9:3489-94. 2009.... - Diazonium-protein adducts for graphite electrode microarrays modification: direct and addressed electrochemical immobilizationBenjamin P Corgier
, UMR 5013 EMB2, , , 43, Boulevard du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
J Am Chem Soc 127:18328-32. 2005..A promising strategy to immobilize markedly different biological entities was then presented, providing an excellent spatial specificity of the electro-addressing... - Protein microarrays enhanced performance using nanobeads arraying and polymer coatingC A Marquette
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR CNRS 5013 Bat CPE, Universite Claude Bernard Lyon 1, 43 Bd du 11 Nov 1918, 69622 Villeurbanne Cedex, France
Talanta 71:1312-8. 2007..Interesting results were obtained, with a RF detection over the 5.3-485IU/ml range and without measurable matrix effect or non-specific signal... - On-chip chemiluminescent signal enhancement using nanostructured gold-modified carbon microarraysBenjamin P Corgier
Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires, Universite Lyon 1 CNRS 5246 ICBMS, Batiment CPE 43, bd du 11 novembre 1918 69622 Villeurbanne, Cedex, France
Langmuir 23:8619-23. 2007..For free PSA and target oligonucleotide detection, enhanced performances were obtained, giving detection limits of 5 ng/mL and 0.1 nM, respectively... - Direct electrochemical addressing of immunoglobulins: immuno-chip on screen-printed microarrayBenjamin P Corgier
Laboratoire de Genie Enzymatique et Biomoleculaire, UMR 5013 EMB2, CNRS Universite Claude Bernard Lyon 1, Bat CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Biosens Bioelectron 22:1522-6. 2007..The absence of non-specific signal or interference problem enabled the detection of RF values in complex samples in the 5.3-485 IU/ml range with a good correlation with the standard Auraflex ELISA test method... - Adhesive microarrays for multipurpose diagnostic toolsBenjamin P Corgier
Laboratoire de Genie Enzymatique, Membranes Biomimetiques et Assemblages Supramoleculaires, Institut de Chimie et Biochimie Moléculaire et Supramoléculaire, Université Lyon1, CNRS 5246 ICBMS, Bat CPE, 43 Bd du 11 Nov, 69622 Villeurbanne, France
Lab Chip 11:3006-10. 2011..The analytical performances of the system were demonstrated for sandwich protein detection (C-reactive protein) and hybridization assays, both in classical 96-well microplate format and microfluidic environment... - Protein-diazonium adduct direct electrografting onto SPRi-biochipBenjamin P Corgier
Laboratoire de Genie Enzymatique et Biomoleculaire, Institut de Chimie et Biochimie Moleculaires et Supramoleculaires Universite Lyon 1, CNRS 5246 ICBMS Batiment CPE, 43, Bd du 11 Novembre 1918, 69622 Villeurbanne, Cedex, France
Langmuir 25:9619-23. 2009..e., 2 nm)... - Electrochemiluminescent biosensors array for the concomitant detection of choline, glucose, glutamate, lactate, lysine and urateChristophe A Marquette
Laboratoire de Génie Enzymatique et Biomoléculaire UMR 5013 CNRS Université Claude Bernard Lyon 1 Bât CPE 43 Bd 11 Nov 1918, 69622 Villeurbanne Cedex, France
Biosens Bioelectron 19:433-9. 2003..Good correlations between measured and expected values were found without the need of internal calibration of the sample, demonstrating the potentiality of the ECL multifunctional bio-sensing chip... - Cylinder-shaped conducting polypyrrole for labelless electrochemical multidetection of DNAAurélie Bouchet
UMR 2714 CNRS bioMérieux, ENS, IFR 128 Biosciences Lyon Gerland, 46 allee d Italie, Lyon, France
Biosens Bioelectron 23:735-40. 2007..It was indeed possible to directly follow the hybridization. Complementary DNA detection limit reached 100 pM (3 fmol in 30 microL), which represents a good performance for such a practical, labelless and real-time sensor...