Research Topics
Genomes and GenesSpecies | Thierry LorcaSummaryCountry: France Publications
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Publications
The Greatwall kinase: a new pathway in the control of the cell cycleT Lorca
Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, Universités Montpellier 2 et 1, Montpellier, France
Oncogene 32:537-43. 2013..These new findings support the view that Gwl, Arpp19 and ENSA could function as the core of a new signalization pathway that, by targeting different final substrates, could participate in a variety of physiological functions...
Constant regulation of both the MPF amplification loop and the Greatwall-PP2A pathway is required for metaphase II arrest and correct entry into the first embryonic cell cycleThierry Lorca
Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
J Cell Sci 123:2281-91. 2010....
The substrate of Greatwall kinase, Arpp19, controls mitosis by inhibiting protein phosphatase 2AAicha Gharbi-Ayachi
Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
Science 330:1673-7. 2010..Although both proteins can inhibit PP2A, endogenous Arpp19, but not α-Endosulfine, is responsible for PP2A inhibition at mitotic entry in Xenopus egg extracts...
Xkid is degraded in a D-box, KEN-box, and A-box-independent pathwayAnna Castro
Centre de Recherche de Biochimie Macromoleculaire, CNRS UPR 1086, 34293 Montpellier Cedex 5, France
Mol Cell Biol 23:4126-38. 2003..Finally, we demonstrate that both a D-box and a GXEN-containing peptides completely block APC-dependent degradation of cyclin B and Xkid, indicating that the GXEN domain might mediate the recognition and association of Xkid with the APC...
Greatwall maintains mitosis through regulation of PP2ASuzanne Vigneron
Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, Labellisée Ligue Nationale Contre le Cancer, Universités Montpellier 2 et 1, Montpellier, France
EMBO J 28:2786-93. 2009..These results suggest that mitotic entry and maintenance is not only mediated by the activation of cyclin B-Cdc2 but also by the regulation of PP2A by GW...
Pin1 stabilizes Emi1 during G2 phase by preventing its association with SCF(betatrcp)Cyril Bernis
Centre de Recherche de Biochimie Macromoleculaire, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
EMBO Rep 8:91-8. 2007..We propose that S- and M-phase entries are mediated by the accumulation of cyclin A and cyclin B through a Pin1-dependent stabilization of Emi1 during G2...
Loss of human Greatwall results in G2 arrest and multiple mitotic defects due to deregulation of the cyclin B-Cdc2/PP2A balanceAndrew Burgess
Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique UMR 5237, 34293 Montpellier Cedex 5, France
Proc Natl Acad Sci U S A 107:12564-9. 2010..These results suggest that the balance between cyclin B-Cdc2 and PP2A must be tightly regulated for correct mitotic entry and exit and that Gwl is crucial for mediating this regulation in somatic human cells...
Kinetochore localization of spindle checkpoint proteins: who controls whom?Suzanne Vigneron
Centre de Recherche de Biochimie Macromoleculaire, Centre National de la Recherche Scientifique Formation de Recherche en Evolution 2593, 34293 Montpellier Cedex 5, France
Mol Biol Cell 15:4584-96. 2004..Thus, at the last step of the spindle checkpoint cascade, Mad1/Mad2, Cdc20, and APC are recruited at the kinetochores independently from each other...
Characterization of the mechanisms controlling Greatwall activitySuzanne Vigneron
Universités Montpellier 2 et 1, Centre de Recherche de Biochimie Macromoleculaire, CNRS UMR 5237, IFR 122, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
Mol Cell Biol 31:2262-75. 2011..In summary, our results identify Gwl as a member of the AGC family of kinases that appears to be regulated by unique mechanisms and that differs from the other members of this family...
The anaphase-promoting complex: a key factor in the regulation of cell cycleAnna Castro
Centre de Recherche de Biochimie Macromoleculaire, CNRS FRE 2593 1919 Route de Mende, 34293 Montpellier Cedex 5, France
Oncogene 24:314-25. 2005..We pay particular attention to the latest results concerning APC structure, APC regulation and substrate recognition, and we discuss the implication of these findings in the understanding the APC function...
The D-Box-activating domain (DAD) is a new proteolysis signal that stimulates the silent D-Box sequence of Aurora-AAnna Castro
, CNRS UPR 1086, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
EMBO Rep 3:1209-14. 2002..The first one, which we designated D-Box-activating domain, within the N-terminal domain of Aurora-A confers the functionality to the second, a silent D-Box, present within the C-terminus of the kinase...
Involvement of Aurora A kinase during meiosis I-II transition in Xenopus oocytesAnna Castro
, UPR 1086 CNRS, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
J Biol Chem 278:2236-41. 2003..Moreover, these oocytes are able to undergo parthenogenetic activation. We conclude that Aurora A and Eg5 are involved in meiosis I to meiosis II transition in Xenopus oocytes...
MyoD undergoes a distinct G2/M-specific regulation in muscle cellsSabrina Batonnet-Pichon
Laboratoire de Génomique Fonctionnelle et Myogenèse, UMR 866 Différenciation, Cellulaire et Croissance, INRA UM II, Campus INRA ENSA, 2 Place Pierre Viala, 34060, Montpellier, Cedex 1, France
Exp Cell Res 312:3999-4010. 2006....
Bovine papillomavirus replicative helicase E1 is a target of the ubiquitin ligase APCFrancisca Mechali
, CNRS FRE 2593, IFR 122, 34 293 Montpellier Cedex 5, France
J Virol 78:2615-9. 2004..These findings involve APC as the ubiquitin ligase that controls E1 levels to maintain a constant low copy number of the viral genome during latent infection...
Ubiquitin-mediated protein degradation in Xenopus egg extractsAnna Castro
, Montpellier, France
Methods Mol Biol 322:223-34. 2006..Finally, the Notes provide a series of practical details that explain the different problems that can occur and the possible solutions used to overcome them...
APC/Fizzy-Related targets Aurora-A kinase for proteolysisAnna Castro
, CNRS UPR 1086, 1919 Route de Mende, F-34293 Montpellier Cedex 5, France
EMBO Rep 3:457-62. 2002..Aurora-A is thus the first protein, at least in our assay system, that undergoes a D-Box-dependent degradation mediated by APC/Fizzy-Related but not by APC/Fizzy...
Multiple phosphorylation events control mitotic degradation of the muscle transcription factor Myf5Christine Doucet
Centre de Recherches de Biochimie Macromoleculaire, CNRS FRE 2593, Montpellier, France
BMC Biochem 6:27. 2005..To this aim, we undertook the biochemical analysis of the mechanisms governing Myf5 mitotic degradation, using heterologous systems...
Exploring meiotic division in Cargèse. Meeting on meiotic divisions and checkpointsAnna Castro
, CNRS FRE 2593, 1919 Route de Mende, 34293 Montpellier Cedex 5, France
EMBO Rep 6:821-5. 2005
CDK-dependent potentiation of MPS1 kinase activity is essential to the mitotic checkpointVioleta Morin
Universite Montpellier, CRBM, 34293 Montpellier, France
Curr Biol 22:289-95. 2012....
