Nicolas Leulliot

Summary

Country: France

Publications

  1. ncbi A structural genomics initiative on yeast proteins
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    J Synchrotron Radiat 10:4-8. 2003
  2. pmc Crystal structure of yeast YER010Cp, a knotable member of the RraA protein family
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    Protein Sci 14:2751-8. 2005
  3. pmc The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    Nucleic Acids Res 36:629-39. 2008
  4. ncbi The box H/ACA RNP assembly factor Naf1p contains a domain homologous to Gar1p mediating its interaction with Cbf5p
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    J Mol Biol 371:1338-53. 2007
  5. ncbi Structure of the yeast tRNA m7G methylation complex
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, IFR115, Orsay Cedex, France
    Structure 16:52-61. 2008
  6. pmc Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll fold
    Cong Zhao Zhou
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8619, Universite Paris Sud, 91405 Orsay, France
    Protein Sci 14:209-15. 2005
  7. ncbi Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    Mol Cell 23:413-24. 2006
  8. ncbi Cloning, production, and purification of proteins for a medium-scale structural genomics project
    Sophie Quevillon-Cheruel
    Institut de Biochimie et Biophysique Moleculaire et Cellulaire, Universite de Paris Sud, Orsay, France
    Methods Mol Biol 363:21-37. 2007
  9. doi Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, CNRS UMR8619, Universite de Paris Sud, Bat 430, 91405 Orsay Cedex, France
    J Mol Biol 398:641-6. 2010
  10. ncbi Structure-based functional annotation: yeast ymr099c codes for a D-hexose-6-phosphate mutarotase
    Marc Graille
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, CNRS UMR8619, F 91405 Orsay cedex, France
    J Biol Chem 281:30175-85. 2006

Collaborators

Detail Information

Publications38

  1. ncbi A structural genomics initiative on yeast proteins
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    J Synchrotron Radiat 10:4-8. 2003
    ..Experimental strategies, first results and identified bottlenecks are presented. The actual or potential contributions to the structural genomics of several experimental structure-determination methods are discussed...
  2. pmc Crystal structure of yeast YER010Cp, a knotable member of the RraA protein family
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    Protein Sci 14:2751-8. 2005
    ..A tunnel at the interface between two monomers, lined with conserved charged residues, has unassigned residual electron density and may constitute an active site for a yet unknown activity...
  3. pmc The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferases
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    Nucleic Acids Res 36:629-39. 2008
    ..We conclude that the catalytic activity of Emg1 is not essential and that the presence of the protein is both necessary and sufficient for ribosome biogenesis...
  4. ncbi The box H/ACA RNP assembly factor Naf1p contains a domain homologous to Gar1p mediating its interaction with Cbf5p
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    J Mol Biol 371:1338-53. 2007
    ..The structural homology with Gar1 suggests that the function of Naf1 involves preventing non-cognate RNAs from being loaded during transport of the particle by inducing a non-productive conformation of Cbf5...
  5. ncbi Structure of the yeast tRNA m7G methylation complex
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, IFR115, Orsay Cedex, France
    Structure 16:52-61. 2008
    ....
  6. pmc Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll fold
    Cong Zhao Zhou
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8619, Universite Paris Sud, 91405 Orsay, France
    Protein Sci 14:209-15. 2005
    ..The presence of a hydrophobic ligand in a well conserved pocket inside the barrel and local sequence similarity with bacterial epimerases may suggest a biochemical function for this protein...
  7. ncbi Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
    Mol Cell 23:413-24. 2006
    ..Structure-guided mutational studies showed that in vivo PTPA activity is influenced by mutations on the surface of the peptide binding pocket, the same mutations that also influenced the in vitro activation of PP2Ai and PPIase activity...
  8. ncbi Cloning, production, and purification of proteins for a medium-scale structural genomics project
    Sophie Quevillon-Cheruel
    Institut de Biochimie et Biophysique Moleculaire et Cellulaire, Universite de Paris Sud, Orsay, France
    Methods Mol Biol 363:21-37. 2007
    ..A limited proteolysis protocol, developed to localize flexible regions in proteins that could hinder crystallization, is also described...
  9. doi Crystal structure of yeast FAD synthetase (Fad1) in complex with FAD
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, CNRS UMR8619, Universite de Paris Sud, Bat 430, 91405 Orsay Cedex, France
    J Mol Biol 398:641-6. 2010
    ....
  10. ncbi Structure-based functional annotation: yeast ymr099c codes for a D-hexose-6-phosphate mutarotase
    Marc Graille
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, CNRS UMR8619, F 91405 Orsay cedex, France
    J Biol Chem 281:30175-85. 2006
    ..The metabolic role of a hexose-6-phosphate mutarotase is discussed. This work illustrates that structural information has been crucial to assign YMR099cp to the orphan EC activity: hexose-phosphate mutarotase...
  11. pmc The H/ACA RNP assembly factor SHQ1 functions as an RNA mimic
    Hélène Walbott
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite de Paris Sud, Orsay Cedex, France
    Genes Dev 25:2398-408. 2011
    ..We propose that Shq1p operates as a Cbf5p chaperone during RNP assembly by acting as an RNA placeholder, thereby preventing Cbf5p from nonspecific RNA binding before association with an H/ACA RNA and the other core RNP proteins...
  12. ncbi Structural characterization of Set1 RNA recognition motifs and their role in histone H3 lysine 4 methylation
    Lionel Trésaugues
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619 CNRS, Universite de Paris Sud, Orsay, France
    J Mol Biol 359:1170-81. 2006
    ..Taken together, our results suggest that Set1 RRMs bind RNA, but Set1 RNA-binding activity is not linked to H3K4 methylation...
  13. ncbi Structure of protein phosphatase methyltransferase 1 (PPM1), a leucine carboxyl methyltransferase involved in the regulation of protein phosphatase 2A activity
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Bat 430, 91405 Orsay, France
    J Biol Chem 279:8351-8. 2004
    ..The structure of PPM1 in a second crystal form provides clues to the dynamic nature of the PPM1/PP2A interaction...
  14. pmc Prp43p contains a processive helicase structural architecture with a specific regulatory domain
    Hélène Walbott
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite de Paris Sud, CNRS UMR8619, IFR115, Orsay Cedex, France
    EMBO J 29:2194-204. 2010
    ..We propose that the C-terminal domain, specific to DEAH/RHA helicases, is a central player in the regulation of helicase activity by binding both RNA and G-patch domain proteins...
  15. pmc Crystal structure of AFV1-102, a protein from the acidianus filamentous virus 1
    Jenny Keller
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, UMR8619 CNRS, 91405 Orsay, France
    Protein Sci 18:845-9. 2009
    ..Although no function can be proposed from structural analysis, tight binding of the gateway tag peptide in a groove between the two motifs suggests AFV1-102 is involved in protein protein interactions...
  16. ncbi Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate
    Cong Zhao Zhou
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS Unité Mixte de Recherche 8619, Universite Paris Sud, Bat 430, 91405 Orsay, France
    J Biol Chem 278:50371-6. 2003
    ..The structure of the free and bound protein are globally similar but show some significant differences in a region containing a polyproline peptide and a putative membrane attachment site...
  17. pmc Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses
    Jenny Keller
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, CNRS UMR 8619, Université Paris 11, IFR115, Batiment 430, 91405 Orsay, France
    Virol J 4:12. 2007
    ..Fluorescence quenching titrations also showed that AFV3-109 interacts with dsDNA. Genomic sequence analysis revealed bacterial homologs of AFV3-109 as a part of a putative previously unidentified prophage sequences in some Firmicutes...
  18. doi Evf, a virulence factor produced by the Drosophila pathogen Erwinia carotovora, is an S-palmitoylated protein with a new fold that binds to lipid vesicles
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, UMR8619 CNRS, IFR115, F 91405 Orsay, France
    J Biol Chem 284:3552-62. 2009
    ..Fluorescence studies of the sole tryptophan residue (Trp94) demonstrated that Evf was indeed able to bind to model membranes containing negatively charged phospholipids and to promote their aggregation...
  19. pmc The family X DNA polymerase from Deinococcus radiodurans adopts a non-standard extended conformation
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite de Paris Sud, CNRS, UMR8619, IFR115, Bat 430, Orsay 91405 Cedex, France
    J Biol Chem 284:11992-9. 2009
    ....
  20. pmc An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitro
    Arnaud Hecker
    Institut de Genetique et Microbiologie, Univ Paris Sud, IFR115, UMR8621 CNRS, 91405 Orsay, France
    Nucleic Acids Res 35:6042-51. 2007
    ..Kae1 is thus a novel and atypical universal DNA interacting protein whose importance could rival those of RecA (RadA/Rad51) in the maintenance of genome integrity in all living cells...
  21. ncbi High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    Acta Crystallogr D Biol Crystallogr 61:664-70. 2005
    ..This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples...
  22. pmc The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)-methyltransferase provides insights into its tRNA specificity
    Hélène Walbott
    Enzymology and Structural Biochemistry Laboratory, CNRS, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Nucleic Acids Res 36:4929-40. 2008
    ..We also predict that a large TPsiC loop conformational change has to occur for the flipping of the target uridine into the (Pab)TrmU54 active site during catalysis...
  23. pmc Crystal structure of the yeast His6 enzyme suggests a reaction mechanism
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, 91405 Orsay, France
    Protein Sci 15:1516-21. 2006
    ..Asp9 is conserved in yeast His6 and bacterial or archaeal HisA sequences, and Asp134 has equivalents in both HisA and TrpF, but they occur at a different position in the protein sequence...
  24. ncbi Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiae
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    J Biol Chem 279:619-25. 2004
    ..This hypothesis is supported by previously published spectroscopic measurements implying that the enzyme undergoes considerable structural changes upon binding of both FMN and EPSP...
  25. ncbi Crystal structure of the YGR205w protein from Saccharomyces cerevisiae: close structural resemblance to E. coli pantothenate kinase
    Ines Li de La Sierra-Gallay
    Laboratoire d Enzymologie et Biochimie Structurales CNRS UPR 9063, Bat 34, 1 Av de la Terrasse, 91198 Gif sur Yvette, France
    Proteins 54:776-83. 2004
    ..The nature of the phosphate accepting substrate remains to be determined...
  26. ncbi Crystal structure of the YDR533c S. cerevisiae protein, a class II member of the Hsp31 family
    Marc Graille
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR 9063, Batiment 34, 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
    Structure 12:839-47. 2004
    ..Detailed structural analysis strongly suggests that the YDR533c protein crystal structure is the first one of a class II member of the Hsp31 family...
  27. ncbi Crystal structure and functional characterization of yeast YLR011wp, an enzyme with NAD(P)H-FMN and ferric iron reductase activities
    Dominique Liger
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS Unité Mixte de Recherche UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    J Biol Chem 279:34890-7. 2004
    ..OY1-2 azoreductase. We show that YLR011wp has an NAD(P)H-dependent FMN reductase and a strong ferricyanide reductase activity. We further demonstrate a weak but specific reductive activity on azo dyes and nitrocompounds...
  28. pmc A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse III
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Orsay, France
    EMBO J 23:2468-77. 2004
    ..The results suggest that this helix allows the Rnt1p dsRBD to bind to short RNA stem-loops by modulating the conformation of helix alpha1, a key RNA-recognition element of the dsRBD...
  29. ncbi The Paris-Sud yeast structural genomics pilot-project: from structure to function
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    Biochimie 86:617-23. 2004
    ..Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation...
  30. pmc Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase family
    Sophie Quevillon-Cheruel
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    Protein Sci 14:1350-6. 2005
    ..This finding was further strengthened by the unexpected presence of a compound covalently bound to the catalytic serine in the active site. Apparently, the enzyme was trapped with a reactive compound during the purification process...
  31. ncbi Crystal structure and confirmation of the alanine:glyoxylate aminotransferase activity of the YFL030w yeast protein
    Philippe Meyer
    Laboratoire d Enzymologie et Biochimie Structurales CNRS UPR 9063 Bât 34, 1, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France
    Biochimie 87:1041-7. 2005
    ..44), an enzyme involved in the hereditary kidney stone disease primary hyperoxaluria type 1. In this paper we show that YFL030w codes for an alanine:glyoxylate aminotransferase, highly specific for its amino donor and acceptor substrates...
  32. ncbi Structure of the human multidrug resistance protein 1 nucleotide binding domain 1 bound to Mg2+/ATP reveals a non-productive catalytic site
    Odile Ramaen
    Institut de Chimie des Substances Naturelles, UPR 2301, Centre National de la Recherche Scientifique, Avenue de la Terrasse, 91190 Gif sur Yvette, France
    J Mol Biol 359:940-9. 2006
    ....
  33. ncbi HalX: an open-source LIMS (Laboratory Information Management System) for small- to large-scale laboratories
    Jaime Prilusky
    Israel Structural Proteomics Center, Weizmann Institute of Science, Rehovot 76100, Israel
    Acta Crystallogr D Biol Crystallogr 61:671-8. 2005
    ..This should lead to much improved management and tracking of structural genomics experimental data...
  34. ncbi Production and crystallization of protein domains: how useful are disorder predictions ?
    S Quevillon-Cheruel
    Structural Genomics Laboratory, IBBMC, CNRS UMR 8619, IFR 115, Universite Paris Sud, Orsay, France
    Curr Protein Pept Sci 8:151-60. 2007
    ..In this paper, we analyze the results of the over-expression in E. coli and crystallization of these constructs, and compare these with the predictions that can be obtained from our software and from others...
  35. ncbi Crystal structure of YHI9, the yeast member of the phenazine biosynthesis PhzF enzyme superfamily
    Dominique Liger
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Orsay, France
    Proteins 60:778-86. 2005
    ..Evolution of this protein by gene duplication, gene fusion and domain swapping from an ancestral gene containing the "hot dog" fold, identifies the protein as a "kinked double hot dog" fold...
  36. ncbi Crystal structure of yeast allantoicase reveals a repeated jelly roll motif
    Nicolas Leulliot
    Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS Unité Mixte de Recherche 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
    J Biol Chem 279:23447-52. 2004
    ..The hexameric packing concentrates these patches into conserved pockets that probably constitute the active site...
  37. pmc Crystallization and preliminary X-ray diffraction analysis of protein 14 from Sulfolobus islandicus filamentous virus (SIFV)
    Adeline Goulet
    Architecture et Fonction des Macromolecules Biologiques, CNRS and Universités d Aix Marseille I et II, UMR 6098, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:884-6. 2006
    ..1, c = 132.4 A. Diffraction data were collected to a maximum acceptable resolution of 2.95 and 3.20 A for the SeMet-labelled and native protein, respectively...
  38. pmc The solution structure of an essential stem-loop of human telomerase RNA
    Thomas Leeper
    Department of Biochemistry, University of Washington, Seattle WA 98195 1700, USA
    Nucleic Acids Res 31:2614-21. 2003
    ..The functional role of this stem-loop appears to be conserved in even distantly related organisms such as yeast and ciliates...