Sebastien Leon

Summary

Country: France

Publications

  1. doi Ubiquitin ligase adaptors: regulators of ubiquitylation and endocytosis of plasma membrane proteins
    Sebastien Leon
    Institut Jacques Monod, UMR 7592 Centre National de la Recherche Scienti fi que, Universités Paris 6 et 7, Paris, France
    Exp Cell Res 315:1574-83. 2009
  2. pmc A conserved cysteine residue of Pichia pastoris Pex20p is essential for its recycling from the peroxisome to the cytosol
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego UCSD, La Jolla, California 92093 0322, USA
    J Biol Chem 282:7424-30. 2007
  3. pmc Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies
    Sebastien Leon
    Institut Jacques Monod, Unité Mixte de Recherche 7592 Centre National de la Recherche Scientifique, Universités Paris 6 et 7, 75251 Paris, France
    Mol Biol Cell 19:2379-88. 2008
  4. pmc Two independent pathways traffic the intraperoxisomal peroxin PpPex8p into peroxisomes: mechanism and evolutionary implications
    Lan Zhang
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Mol Biol Cell 17:690-9. 2006
  5. pmc Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093
    J Cell Biol 172:67-78. 2006
  6. pmc A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting
    Zoi Erpapazoglou
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 7592, Universite Paris Diderot, Sorbonne Paris Cité, Paris, France
    Mol Biol Cell 23:2170-83. 2012
  7. doi Versatile role of the yeast ubiquitin ligase Rsp5p in intracellular trafficking
    Naïma Belgareh-Touzé
    Institut Jacques Monod, CNRS, Universités Paris Diderot Paris 7 and UPMC Paris 6, 2 place Jussieu, 75005 Paris, France
    Biochem Soc Trans 36:791-6. 2008
  8. pmc A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis
    Michel Becuwe
    Institut Jacques Monod, Centre National de la Recherche Scientifique, UMR 7592, Universite Paris Diderot, Sorbonne Paris Cité, F 75205 Paris, France
    J Cell Biol 196:247-59. 2012
  9. pmc A deubiquitylating complex required for neosynthesis of a yeast mitochondrial ATP synthase subunit
    Sophie Kanga
    Institut Jacques Monod, CNRS, UMR7592, Univ Paris Diderot, Sorbonne Paris Cité, Paris, France
    PLoS ONE 7:e38071. 2012
  10. ncbi Uniqueness of the mechanism of protein import into the peroxisome matrix: transport of folded, co-factor-bound and oligomeric proteins by shuttling receptors
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University California, Room 3230 Bonner Hall, 9500 Gilman Drive, UC San Diego, La Jolla, CA 92093 0322, USA
    Biochim Biophys Acta 1763:1552-64. 2006

Collaborators

  • Suresh Subramani
  • Lan Zhang
  • Titia K Sixma
  • Ivan Tarassov
  • Joel M Goodman
  • Graca Raposo
  • Fulvio Reggiori
  • Margarida Casal
  • M Veenhuis
  • Olivier Vincent
  • Jean Claude Farre
  • Ravi Manjithaya
  • Taras Y Nazarko
  • Rosine Haguenauer-Tsapis
  • Michel Becuwe
  • Zoi Erpapazoglou
  • Mingda Yan
  • Changle Ma
  • Naganand Rayapuram
  • Uwe Schumann
  • Richard D Mathewson
  • Andriy A Sibirny
  • Sophie Kanga
  • Daniele Urban-Grimal
  • Michael E Baker
  • Naïma Belgareh-Touzé
  • Andriy S Polupanov
  • James M Cregg
  • Oleh V Stasyk
  • Geoffrey Paul Lin-Cereghino
  • Partha P Hazra
  • Muriel Mari
  • Sandra Paiva
  • Jéssica Gomes-Rezende
  • Francesca Mattiroli
  • Delphine Bernard
  • Antonio Herrador
  • Manel Dhaoui
  • Carina Soares-Cunha
  • Marina Pantazopoulou
  • David Lara
  • Francesca Giordano
  • Neide Vieira
  • Anne Marie Mager-Heckel
  • Christophe Anjard
  • Shveta Jain
  • William F Loomis
  • Saurabh Joshi
  • Marta Stawiecka-Mirota
  • Richard A Rachubinski
  • Dorian A Rachubinski
  • Keith Booher
  • Kanae Shirahama-Noda
  • Ravi R Manjithaya
  • Jason Vidal
  • Samone Khuongsathiene
  • Volodymyr Y Nazarko
  • Olena G Stasyk
  • Olena S Krasovska
  • Joan Lin-Cereghino
  • Sabrina Johnson
  • Ilya Tolstorukov
  • Laurie Godfrey
  • Bernard J de la Cruz
  • Ivet Suriapranata
  • William B Snyder

Detail Information

Publications35

  1. doi Ubiquitin ligase adaptors: regulators of ubiquitylation and endocytosis of plasma membrane proteins
    Sebastien Leon
    Institut Jacques Monod, UMR 7592 Centre National de la Recherche Scienti fi que, Universités Paris 6 et 7, Paris, France
    Exp Cell Res 315:1574-83. 2009
    ..Finally, because these adaptors represent an additional layer of specificity in the ubiquitylation cascade, and can themselves be subject to a complex regulation, they are essential actors in the fine-tuning of endocytosis...
  2. pmc A conserved cysteine residue of Pichia pastoris Pex20p is essential for its recycling from the peroxisome to the cytosol
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego UCSD, La Jolla, California 92093 0322, USA
    J Biol Chem 282:7424-30. 2007
    ..In addition, expression of this mutant protein in wild-type cells confers a dominant-negative, oleate-specific growth defect, which is a useful tool for a better understanding of peroxisomal receptor recycling...
  3. pmc Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies
    Sebastien Leon
    Institut Jacques Monod, Unité Mixte de Recherche 7592 Centre National de la Recherche Scientifique, Universités Paris 6 et 7, 75251 Paris, France
    Mol Biol Cell 19:2379-88. 2008
    ..Therefore, Ear1p/Ssh4p recruit Rsp5p and assist it in its function at MVBs by directing the ubiquitylation of specific cargoes...
  4. pmc Two independent pathways traffic the intraperoxisomal peroxin PpPex8p into peroxisomes: mechanism and evolutionary implications
    Lan Zhang
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Mol Biol Cell 17:690-9. 2006
    ..These data suggest a direct interaction between PpPex8p and Pex20p. Our studies shed light on the mechanism and evolution of the dual import pathways for PpPex8p...
  5. pmc Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093
    J Cell Biol 172:67-78. 2006
    ..Our data are consistent with a model in which Pex20p enters peroxisomes and recycles back to the cytosol in an ubiquitin-dependent manner...
  6. pmc A dual role for K63-linked ubiquitin chains in multivesicular body biogenesis and cargo sorting
    Zoi Erpapazoglou
    Institut Jacques Monod, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 7592, Universite Paris Diderot, Sorbonne Paris Cité, Paris, France
    Mol Biol Cell 23:2170-83. 2012
    ..A failure to generate K63Ub chains in yeast leads to an MVB ultrastructure alteration. Our work thus unravels a double function of K63Ub chains in cargo sorting and MVB biogenesis...
  7. doi Versatile role of the yeast ubiquitin ligase Rsp5p in intracellular trafficking
    Naïma Belgareh-Touzé
    Institut Jacques Monod, CNRS, Universités Paris Diderot Paris 7 and UPMC Paris 6, 2 place Jussieu, 75005 Paris, France
    Biochem Soc Trans 36:791-6. 2008
    ..In the present review, we discuss recent examples illustrating the role of Rsp5p in membrane protein trafficking and providing new insights into the regulation of this E3 by adaptor proteins...
  8. pmc A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis
    Michel Becuwe
    Institut Jacques Monod, Centre National de la Recherche Scientifique, UMR 7592, Universite Paris Diderot, Sorbonne Paris Cité, F 75205 Paris, France
    J Cell Biol 196:247-59. 2012
    ....
  9. pmc A deubiquitylating complex required for neosynthesis of a yeast mitochondrial ATP synthase subunit
    Sophie Kanga
    Institut Jacques Monod, CNRS, UMR7592, Univ Paris Diderot, Sorbonne Paris Cité, Paris, France
    PLoS ONE 7:e38071. 2012
    ..The defect appears at the level of ATP9 mRNA translation, while its maturation remained unchanged in the mutants. This study describes a new role of the ubiquitin system in mitochondrial biogenesis...
  10. ncbi Uniqueness of the mechanism of protein import into the peroxisome matrix: transport of folded, co-factor-bound and oligomeric proteins by shuttling receptors
    Sebastien Leon
    Section of Molecular Biology, Division of Biological Sciences, University California, Room 3230 Bonner Hall, 9500 Gilman Drive, UC San Diego, La Jolla, CA 92093 0322, USA
    Biochim Biophys Acta 1763:1552-64. 2006
    ....
  11. ncbi Characterization of protein-protein interactions: application to the understanding of peroxisome biogenesis
    Sebastien Leon
    Section of Molecular Biology, University of California San Diego, La Jolla, CA, USA
    Methods Mol Biol 389:219-38. 2007
    ..This chapter discusses methods that have been used for the study of such interactions among both soluble and membrane-associated proteins in peroxisome biogenesis. The procedures are equally applicable to other cellular processes...
  12. pmc Ubiquitin-mediated regulation of endocytosis by proteins of the arrestin family
    Michel Becuwe
    Institut Jacques Monod, Centre National de la Recherche Scientifique, UMR 7592, Universite Paris Diderot, Sorbonne Paris Cité, 75205 Paris, France
    Biochem Res Int 2012:242764. 2012
    ..In this paper, we discuss recent data on the intricate connections between arrestins and the ubiquitin pathway in the control of endocytosis...
  13. pmc The peroxisomal matrix import of Pex8p requires only PTS receptors and Pex14p
    Changle Ma
    University of California, San Diego, La Jolla, 92093 0322, USA
    Mol Biol Cell 20:3680-9. 2009
    ..Our data support the idea that PpPex8p is a special cargo whose translocation into peroxisomes depends only on the PTS receptors and Pex14p and not on intraperoxisomal Pex8p, the RING subcomplex, or the receptor-recycling machinery...
  14. pmc Unconventional secretion of Pichia pastoris Acb1 is dependent on GRASP protein, peroxisomal functions, and autophagosome formation
    Ravi Manjithaya
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 188:537-46. 2010
    ..Moreover, yeast Acb1 and several proteins required for its secretion are necessary for sporulation in P. pastoris. Our findings implicate currently unknown, evolutionarily conserved pathways in unconventional secretion...
  15. pmc PpAtg30 tags peroxisomes for turnover by selective autophagy
    Jean Claude Farre
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, San Diego, CA 92093 0322, USA
    Dev Cell 14:365-76. 2008
    ..Therefore, PpAtg30 is a key player in the selection of peroxisomes as cargo and in their delivery to the autophagy machinery for pexophagy...
  16. pmc Dysferlin domain-containing proteins, Pex30p and Pex31p, localized to two compartments, control the number and size of oleate-induced peroxisomes in Pichia pastoris
    Mingda Yan
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Mol Biol Cell 19:885-98. 2008
    ..The growth environment-specific role of these peroxins, their dual localization, and the function of their dysferlin domains provide novel insights into peroxisome morphogenesis...
  17. ncbi Peroxisome remnants in pex3delta cells and the requirement of Pex3p for interactions between the peroxisomal docking and translocation subcomplexes
    Partha P Hazra
    Section of Molecular Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Traffic 3:560-74. 2002
    ..We discuss roles for Pex3p in the assembly of specific peroxisomal membrane protein subcomplexes whose formation is necessary for matrix protein import...
  18. ncbi Autophagy-related pathways and specific role of sterol glucoside in yeasts
    Taras Y Nazarko
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0322, USA
    Autophagy 3:263-5. 2007
    ..Therefore, we speculate that SG might facilitate elongation of any double membrane from the PAS and this enhancer function of SG becomes essential when extremely large double membranes are formed...
  19. ncbi Peroxisome turnover by micropexophagy: an autophagy-related process
    Jean Claude Farre
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Trends Cell Biol 14:515-23. 2004
    ....
  20. ncbi A cytoplasm to vacuole targeting pathway in P. pastoris
    Jean Claude Farre
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093 0322, USA
    Autophagy 3:230-4. 2007
    ..The discovery of a Cvt pathway in P. pastoris makes it an excellent model system for the dissection of autophagy-related pathways in a single organism and for the discovery of new Cvt pathway components...
  21. pmc Peroxisome size provides insights into the function of autophagy-related proteins
    Taras Y Nazarko
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Mol Biol Cell 20:3828-39. 2009
    ..As a rule, the larger the cargo, the more cargo-specific Atg proteins become essential for its sequestration...
  22. ncbi The importomer--a peroxisomal membrane complex involved in protein translocation into the peroxisome matrix
    Naganand Rayapuram
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, Room 3230 Bonner Hall, 9500 Gilman Drive, UC San Diego, La Jolla, CA 92093 0322, USA
    Biochim Biophys Acta 1763:1613-9. 2006
    ..We provide below a brief historical perspective regarding the importomer and its role in peroxisome biogenesis. We also identify areas in which further work is needed to uncover the physiological role of the importomer...
  23. ncbi Localization of proteins and organelles using fluorescence microscopy
    Jean Claude Farre
    Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA
    Methods Mol Biol 389:239-50. 2007
    ..Also included are protocols for the labeling of different subcellular organelles with vital stains...
  24. pmc Roles of Pichia pastoris Uvrag in vacuolar protein sorting and the phosphatidylinositol 3-kinase complex in phagophore elongation in autophagy pathways
    Jean Claude Farre
    Section of Molecular Biology, Division of Biological Sciences, University California, San Diego, CA, USA
    Autophagy 6:86-99. 2010
    ..Rather, the defects in all autophagic pathways observed when PpUvrag was overexpressed support its presence in a complex that competes with the PI3K complex required for autophagy...
  25. pmc Hitchhiking fads en route to peroxisomes
    Suresh Subramani
    Section of Molecular Biology, Division of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 156:415-7. 2002
    ..A paper in this issue addresses, in a very thorough fashion, the assembly, cofactor binding, and import of an oligomeric protein, acyl-CoA oxidase (Aox), into the peroxisome matrix (Titorenko et al., 2002, this issue)...
  26. pmc Peroxisome matrix and membrane protein biogenesis
    Changle Ma
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    IUBMB Life 61:713-22. 2009
    ..In this manuscript we review the recent progress made in our understanding of the mechanisms by which matrix and membrane proteins are sorted to and assembled into peroxisomes...
  27. pmc The requirement of sterol glucoside for pexophagy in yeast is dependent on the species and nature of peroxisome inducers
    Taras Y Nazarko
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    Mol Biol Cell 18:106-18. 2007
    ....
  28. ncbi A ubiquitin-like protein involved in membrane fusion
    Suresh Subramani
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, CA 92093, USA
    Cell 130:18-20. 2007
    ..2007) show that Atg8 conjugated to PE mediates tethering between adjacent membranes and stimulates membrane hemifusion, an event that may mimic expansion of the autophagosomal membrane during autophagy...
  29. ncbi The control of peroxisome number and size during division and proliferation
    Mingda Yan
    Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA 92093 0322, USA
    Curr Opin Cell Biol 17:376-83. 2005
    ..Related DRPs also function in the division of mitochondria and chloroplasts. Many other proteins and signals regulate peroxisome division and proliferation, but their modes of action are still being studied...
  30. pmc A yeast MAPK cascade regulates pexophagy but not other autophagy pathways
    Ravi Manjithaya
    Section of Molecular Biology, University of California, San Diego, La Jolla, CA 92093, USA
    J Cell Biol 189:303-10. 2010
    ..We propose that pexophagy requires the simultaneous activation of this MAPK pathway and a hexose-sensing mechanism acting through protein kinase A and cyclic adenosine monophosphate...
  31. pmc Molecular mechanism and physiological role of pexophagy
    Ravi Manjithaya
    Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093 0322, USA
    FEBS Lett 584:1367-73. 2010
    ..These insights and additional observations in the literature provide a framework for an understanding of the physiological role(s) of pexophagy...
  32. pmc Special delivery from mitochondria to peroxisomes
    Uwe Schumann
    Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA
    Trends Cell Biol 18:253-6. 2008
    ..We discuss the potential role of this vesicle population in the context of pathways for the exchange of metabolites and/or macromolecules between these compartments...
  33. pmc Mxr1p, a key regulator of the methanol utilization pathway and peroxisomal genes in Pichia pastoris
    Geoffrey Paul Lin-Cereghino
    Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, 2000 N W Walker Road, Beaverton, Oregon 97006, USA
    Mol Cell Biol 26:883-97. 2006
    ..We propose that MXR1 is the P. pastoris homologue of S. cerevisiae ADR1 but that it has gained new functions and lost others through evolution as a result of changes in the spectrum of genes that it controls...
  34. ncbi Atg28, a novel coiled-coil protein involved in autophagic degradation of peroxisomes in the methylotrophic yeast Pichia pastoris
    Oleh V Stasyk
    Institute of Cell Biology, National Academy of Sciences of Ukraine, Lviv, Ukraine
    Autophagy 2:30-8. 2006
    ..The identification of ATG28 suggests that pexophagy may involve species-specific components, since this gene appears to have only weak homologues in other yeasts...
  35. doi Activity versus peroxisomal targeting of PerCR
    Michael E Baker
    Structure 16:331-2. 2008