Emmanuel Lemichez

Summary

Country: France

Publications

  1. ncbi request reprint E. coli CNF1 toxin: a two-in-one system for host-cell invasion
    Luce Landraud
    INSERM U452, Faculté de Médecine de Nice, Nice, France
    Int J Med Microbiol 293:513-8. 2004
  2. pmc Induction of transient macroapertures in endothelial cells through RhoA inhibition by Staphylococcus aureus factors
    Laurent Boyer
    Toxines Bactériennes dans la Relation Hôte Pathogènes, U627, Faculte de Medecine, Institut National de la Sante et de la Recherche Medicale, INSERM, 06107 Nice Cedex 2, France
    J Cell Biol 173:809-19. 2006
  3. doi request reprint Cholera toxin notches epithelial junctions
    Emmanuel Lemichez
    INSERM U1065, Centre Méditerranéen de Médecine Moléculaire, C3M, Universite de Nice Sophia Antipolis, 151 Route St Antoine de Ginestière, BP 2 3194, 06204 Nice Cedex, France Electronic address
    Cell Host Microbe 14:227-9. 2013
  4. doi request reprint Hijacking of Rho GTPases during bacterial infection
    Emmanuel Lemichez
    INSERM U1065, Equipe Labellisée Ligue contre le Cancer, Centre Méditerranéen de Médecine Moléculaire C3M, Universite de Nice Sophia Antipolis, 06204 Cedex 3 Nice, France
    Exp Cell Res 319:2329-36. 2013
  5. pmc General aspects and recent advances on bacterial protein toxins
    Emmanuel Lemichez
    INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire, Microbial Toxins in Host Pathogen Interactions, C3M, Universite de Nice Sophia Antipolis, UFR Medecine, 06204 Nice, France
    Cold Spring Harb Perspect Med 3:a013573. 2013
  6. ncbi request reprint Response from Emmanuel Lemichez: A new turn in Rho GTPase activation by Escherichia coli cytotoxic necrotizing factors
    Emmanuel Lemichez
    INSERM U452, IFR50, Faculty of Medicine, 28 Avenue de Valombrose, 06107 Nice, France
    Trends Microbiol 11:156. 2003
  7. doi request reprint Breaking the wall: targeting of the endothelium by pathogenic bacteria
    Emmanuel Lemichez
    INSERM, U895, Toxines Microbiennes dans la Relation Hôte Pathogènes, Centre Méditerranéen de Médecine Moléculaire, C3M, Nice, 06204, Cedex 3, France
    Nat Rev Microbiol 8:93-104. 2010
  8. ncbi request reprint Activation and proteasomal degradation of rho GTPases by cytotoxic necrotizing factor-1 elicit a controlled inflammatory response
    Patrick Munro
    INSERM, U627, Faculte de Medecine, 28 Ave de Valombrose, 06107 Nice Cedex 2, France
    J Biol Chem 279:35849-57. 2004
  9. ncbi request reprint CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion
    Anne Doye
    INSERM U452, IFR 50, Faculte de Medecine, 28 Avenue de Valombrose, 06107, Nice, France
    Cell 111:553-64. 2002
  10. doi request reprint The E3 ubiquitin-ligase HACE1 catalyzes the ubiquitylation of active Rac1
    Stéphanie Torrino
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes, 06204 Cedex 3 Nice, France
    Dev Cell 21:959-65. 2011

Collaborators

Detail Information

Publications41

  1. ncbi request reprint E. coli CNF1 toxin: a two-in-one system for host-cell invasion
    Luce Landraud
    INSERM U452, Faculté de Médecine de Nice, Nice, France
    Int J Med Microbiol 293:513-8. 2004
    ..Finally, we have reported that ubiquitylation and degradation of Rac is lost in HEp-2 carcinoma cells, thereby suggesting a possible link between Rho protein ubiquitylation and tumor progression...
  2. pmc Induction of transient macroapertures in endothelial cells through RhoA inhibition by Staphylococcus aureus factors
    Laurent Boyer
    Toxines Bactériennes dans la Relation Hôte Pathogènes, U627, Faculte de Medecine, Institut National de la Sante et de la Recherche Medicale, INSERM, 06107 Nice Cedex 2, France
    J Cell Biol 173:809-19. 2006
    ..Intoxication of endothelial monolayers by EDIN produces a loss of barrier function and provides direct access of the endothelium basement membrane to S. aureus...
  3. doi request reprint Cholera toxin notches epithelial junctions
    Emmanuel Lemichez
    INSERM U1065, Centre Méditerranéen de Médecine Moléculaire, C3M, Universite de Nice Sophia Antipolis, 151 Route St Antoine de Ginestière, BP 2 3194, 06204 Nice Cedex, France Electronic address
    Cell Host Microbe 14:227-9. 2013
    ..2013) report that CT compromises intestinal epithelium barrier function via cyclic AMP (cAMP)-induced disruption of Rab11- and exocyst-dependent delivery of endocytic recycling cargo to cell-cell junctions...
  4. doi request reprint Hijacking of Rho GTPases during bacterial infection
    Emmanuel Lemichez
    INSERM U1065, Equipe Labellisée Ligue contre le Cancer, Centre Méditerranéen de Médecine Moléculaire C3M, Universite de Nice Sophia Antipolis, 06204 Cedex 3 Nice, France
    Exp Cell Res 319:2329-36. 2013
    ..Finally, a number of studies indicate that toxins targeting Rho proteins have great potential in the development of new therapeutic tools...
  5. pmc General aspects and recent advances on bacterial protein toxins
    Emmanuel Lemichez
    INSERM, U1065, Centre Méditerranéen de Médecine Moléculaire, Microbial Toxins in Host Pathogen Interactions, C3M, Universite de Nice Sophia Antipolis, UFR Medecine, 06204 Nice, France
    Cold Spring Harb Perspect Med 3:a013573. 2013
    ..Understanding the mechanisms of toxin action provides a basis to develop vaccines and therapies to combat bacterial pathogens and to develop new strategies to use toxin derivatives for the treatment of human disease...
  6. ncbi request reprint Response from Emmanuel Lemichez: A new turn in Rho GTPase activation by Escherichia coli cytotoxic necrotizing factors
    Emmanuel Lemichez
    INSERM U452, IFR50, Faculty of Medicine, 28 Avenue de Valombrose, 06107 Nice, France
    Trends Microbiol 11:156. 2003
  7. doi request reprint Breaking the wall: targeting of the endothelium by pathogenic bacteria
    Emmanuel Lemichez
    INSERM, U895, Toxines Microbiennes dans la Relation Hôte Pathogènes, Centre Méditerranéen de Médecine Moléculaire, C3M, Nice, 06204, Cedex 3, France
    Nat Rev Microbiol 8:93-104. 2010
    ....
  8. ncbi request reprint Activation and proteasomal degradation of rho GTPases by cytotoxic necrotizing factor-1 elicit a controlled inflammatory response
    Patrick Munro
    INSERM, U627, Faculte de Medecine, 28 Ave de Valombrose, 06107 Nice Cedex 2, France
    J Biol Chem 279:35849-57. 2004
    ..Consistently, we assume that the CNF1-induced ubiquitin-mediated proteasomal degradation of activated Rho proteins may limit the amplitude of the host cell immune responses...
  9. ncbi request reprint CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion
    Anne Doye
    INSERM U452, IFR 50, Faculte de Medecine, 28 Avenue de Valombrose, 06107, Nice, France
    Cell 111:553-64. 2002
    ..CNF1 toxicity thus results from a restricted activation of Rho GTPases through hijacking the host cell proteasomal machinery...
  10. doi request reprint The E3 ubiquitin-ligase HACE1 catalyzes the ubiquitylation of active Rac1
    Stéphanie Torrino
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes, 06204 Cedex 3 Nice, France
    Dev Cell 21:959-65. 2011
    ..Together, these data establish the role of the HACE1 E3 ubiquitin-ligase in controlling Rac1 ubiquitylation and activity...
  11. ncbi request reprint Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin
    Anne Doye
    INSERM, Faculte de Medecine Nice, France
    Methods Enzymol 406:447-56. 2006
    ..Collectively, these results indicate that association with the cellular membranes is a necessary step for activated-Rac1 ubiquitylation...
  12. doi request reprint Transcriptome dysregulation by anthrax lethal toxin plays a key role in induction of human endothelial cell cytotoxicity
    Monica Rolando
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Nice, 06204 Cedex 3, France
    Cell Microbiol 12:891-905. 2010
    ..Together our data establish that the modulation of the cell transcriptome by LT plays a key role in triggering human endothelial cell toxicity...
  13. pmc CNF1-induced ubiquitylation and proteasome destruction of activated RhoA is impaired in Smurf1-/- cells
    Laurent Boyer
    Faculte de Medecine, Institut National de la Sante et de la Recherche Medicale, U627, 06107 Nice Cedex 2, France
    Mol Biol Cell 17:2489-97. 2006
    ..Thus, both CNF1 and transforming growth factor-beta trigger activated RhoA ubiquitylation through Smurf1 ubiquitin-ligase...
  14. doi request reprint Escherichia coli producing CNF1 toxin hijacks Tollip to trigger Rac1-dependent cell invasion
    Orane Visvikis
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes, Nice, F 06204 Cedex 3, France
    Traffic 12:579-90. 2011
    ..Collectively, these findings reveal a new link between Rac1 and Tollip, Tom1 and clathrin membrane trafficking components hijacked by pathogenic bacteria to allow their efficient invasion of host cells...
  15. pmc The Staphylococcus aureus epidermal cell differentiation inhibitor toxin promotes formation of infection foci in a mouse model of bacteremia
    Patrick Munro
    INSERM, U895, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes, Universite de Nice Sophia Antipolis, UFR Medecine, IFR50, Nice 06204, France
    Infect Immun 78:3404-11. 2010
    ..These results suggest that EDIN is a risk factor for S. aureus dissemination through the vasculature by virtue of its ability to promote the formation of infection foci in deep-seated tissues...
  16. pmc Injection of Staphylococcus aureus EDIN by the Bacillus anthracis protective antigen machinery induces vascular permeability
    Monica Rolando
    INSERM, U895, Equipe 6, C3M, Microbial Toxins in Host Pathogen Interactions, Nice F 06204, France
    Infect Immun 77:3596-601. 2009
    ..This suggests new ways to design therapeutic drugs against anthrax directed toward vascular permeability...
  17. doi request reprint Laminin-binding integrins induce Dll4 expression and Notch signaling in endothelial cells
    Soline Estrach
    INSERM, U634, Nice F 06107, France
    Circ Res 109:172-82. 2011
    ..Because angiogenesis requires a tight coordination of these cellular processes, we hypothesized that adhesion, vascular endothelial growth factor, and Notch signaling pathways are interconnected...
  18. ncbi request reprint Targeting of host-cell ubiquitin and ubiquitin-like pathways by bacterial factors
    Laurent Boyer
    Institut National de la Sante et de la Recherche Medicale INSERM, U627, Faculte de Medecine, 28 Avenue de Valombrose, Nice, 06107 Cedex 2, France
    Nat Rev Microbiol 2:779-88. 2004
  19. pmc Rac GTPase instructs nuclear factor-kappaB activation by conveying the SCF complex and IkBalpha to the ruffling membranes
    Laurent Boyer
    Institut National de la Santé et de la Recherche Médicale U452, IFR50, Faculte de Medecine, 06107 Nice, France
    Mol Biol Cell 15:1124-33. 2004
    ..Consequently, this points to the role of Rac as a docking molecule in NF-kappaB activation...
  20. pmc Modification of Salmonella Typhimurium motility by the probiotic yeast strain Saccharomyces boulardii
    Rodolphe Pontier-Bres
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire C3M, Equipe Inflammation, Cancer, Cellules Souches Cancéreuses, Nice, France
    PLoS ONE 7:e33796. 2012
    ..In case of Salmonella infection, S.b-B has been shown to decrease ST invasion of T84 colon cell line. The present study was designed to investigate the impact of S.b-B on ST motility...
  21. ncbi request reprint Bacteria and the ubiquitin pathway
    Patrick Munro
    INSERM, U627, Faculte de Medecine, 28 Avenue de Valombrose, 06107, Nice, Cedex 2, France
    Curr Opin Microbiol 10:39-46. 2007
    ....
  22. ncbi request reprint Specificity of immunomodulator secretion in urinary samples in response to infection by alpha-hemolysin and CNF1 bearing uropathogenic Escherichia coli
    Jean Michel Real
    INSERM, U627, Faculte de Medecine, 28 Avenue de Valombrose, F 06107 Nice cedex 2, France
    Cytokine 37:22-5. 2007
    ..In addition, we observed that MCP-1, and IL-8 to a lower extent, is produced in urine at higher concentrations in UTIs caused by UPECs carrying alpha-hly/cnf1...
  23. ncbi request reprint The Rho GTPase activators CNF1 and DNT bacterial toxins have mucosal adjuvant properties
    Patrick Munro
    INSERM, U627, Faculte de Medecine, 28 Avenue de Valombrose, F 06107 Nice cedex 2, France
    Vaccine 23:2551-6. 2005
    ..Manipulation of Rho proteins thus provides a possible new approach for the development of effective mucosal immunoadjuvants...
  24. ncbi request reprint Intranasal immunization with tetanus toxoid and CNF1 as a new mucosal adjuvant protects BALB/c mice against lethal challenge
    Patrick Munro
    Institut National de la Sante et de la Recherche Medicale, INSERM, U627, Universite de Nice Sophia Antipolis, Faculte de Medecine, 28 Avenue de Valombrose, F 06107 Nice cedex 2, France
    Vaccine 25:8702-6. 2007
    ..Immunized mice were protected against a challenge with lethal doses of tetanus toxin (10 x LD(50)). The Rho GTPase activating toxin CNF1 is thus an attractive mucosal adjuvant candidate for nasal vaccines...
  25. ncbi request reprint Bacterial virulence factors targeting Rho GTPases: parasitism or symbiosis?
    Patrice Boquet
    INSERM U452, IFR50, Faculty of Medicine 28 Avenue de Valombrose, 06107 Nice, France
    Trends Cell Biol 13:238-46. 2003
    ....
  26. doi request reprint Assessing ubiquitylation of Rho GTPases in mammalian cells
    Anne Doye
    Faculte de Medecine, INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Institut Signalisation et Pathologie, IFR50, Universite de Nice Sophia Antipolis, Nice, France
    Methods Mol Biol 827:77-86. 2012
    ..Here, we describe how to assess the level of ubiquitylation of Rho proteins in cells, taking Rac1 as an example...
  27. doi request reprint cAMP signaling by anthrax edema toxin induces transendothelial cell tunnels, which are resealed by MIM via Arp2/3-driven actin polymerization
    Madhavi P Maddugoda
    INSERM, U, Universite de Nice Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire, Nice, France
    Cell Host Microbe 10:464-74. 2011
    ..Thus, the balance between ET-induced TEM formation and resealing likely determines the integrity of the host endothelium barrier...
  28. pmc Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro
    Gregory Michel
    Universite de Nice Sophia Antipolis, Faculte de Medecine, Nice, France
    PLoS Negl Trop Dis 5:e1323. 2011
    ..This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection...
  29. ncbi request reprint Rho GTPase-activating bacterial toxins: from bacterial virulence regulation to eukaryotic cell biology
    Marc Lemonnier
    INSERM U627, UNSA, Faculte de Medecine, 28 Avenue de Valombrose, 06107 Nice Cedex 2, France
    FEMS Microbiol Rev 31:515-34. 2007
    ....
  30. doi request reprint Autophagy is required for CSF-1-induced macrophagic differentiation and acquisition of phagocytic functions
    Arnaud Jacquel
    INSERM U1065, Centre Méditerranéen de Médecine Moléculaire, Nice, France
    Blood 119:4527-31. 2012
    ..Collectively, these findings highlight an unexpected and essential role of autophagy during monocyte differentiation and acquisition of macrophage functions...
  31. doi request reprint alpha2beta1 integrin controls association of Rac with the membrane and triggers quiescence of endothelial cells
    Laurence Cailleteau
    INSERM, U634, Faculte de Medecine, 27 avenue de Valombrose, Nice, F 06107, France
    J Cell Sci 123:2491-501. 2010
    ..Altogether, our data establish that alpha2beta1 integrin controls endothelial cell commitment towards quiescence by triggering a CD9-dependent dominant signaling...
  32. doi request reprint Direct modifications of Rho proteins: deconstructing GTPase regulation
    Orane Visvikis
    INSERM, U895, Centre Méditerranéen de Médecine Moléculaire, C3M, Toxines Microbiennes dans la Relation Hôte Pathogènes, Nice, F 06204 Cedex 3, France
    Biol Cell 102:377-89. 2010
    ..We review and compare these unconventional ways in which both host cells and bacterial pathogens regulate Rho proteins...
  33. doi request reprint Characterization of a cluster of oncogenic mutations in E6 of a human papillomavirus 83 variant isolated from a high-grade squamous intraepithelial lesion
    Isabelle Cannavo
    Laboratoire de Virologie, Centre Hospitalier Universitaire de Nice, Hopital Archet 2, Nice, France
    J Gen Virol 92:2428-36. 2011
    ..Importantly, our data define the importance of a cluster of mutations in the second zinc finger of E6m in increasing the oncogenic potential of HPV83...
  34. ncbi request reprint Transcellular tunnel dynamics: Control of cellular dewetting by actomyosin contractility and I-BAR proteins
    Emmanuel Lemichez
    INSERM, U1065, Universite de Nice Sophia Antipolis, Centre Méditerranéen de Médecine Moléculaire, C3M, Nice 06204, France
    Biol Cell 105:109-17. 2013
    ....
  35. pmc Interaction of Saccharomyces boulardii with Salmonella enterica serovar Typhimurium protects mice and modifies T84 cell response to the infection
    Flaviano S Martins
    Team 4 Inflammation, Cancer, Cancer Stem Cells, Unité INSERM U895, Centre Méditerranéen de Médecine Moléculaire, Nice, France
    PLoS ONE 5:e8925. 2010
    ..Saccharomyces boulardii is a non-pathogenic yeast used as a probiotic in infectious diarrhea...
  36. pmc Ubiquitylation of active Rac1 by the E3 ubiquitin-ligase HACE1
    Amel Mettouchi
    INSERM, U634, Faculté de Médecine de Nice, Nice, France
    Small Gtpases 3:102-6. 2012
    ..We here review these findings in parallel with the regulation of RhoA by the ubiquitin and proteasome system (UPS) and discuss the impact of these regulations on the capacity of Rho GTPases to signal...
  37. pmc To be helped or not helped, that is the question
    Emmanuel Lemichez
    INSERM U452, Faculty of Medicine, 28 Avenue de Valombrose, 06107 Nice Cedex 2, France
    J Cell Biol 160:991-2. 2003
    ....
  38. ncbi request reprint Cross-talk between RhoGTPases and stress activated kinases for matrix metalloproteinase-9 induction in response to keratinocytes injury
    Laurent Turchi
    INSERM U 385, Nice, France
    J Invest Dermatol 121:1291-300. 2003
    ..The first involves Rac1 and/or Cdc42 that control the activation of p38[MAPK]. The second depends on RhoA activation that is required for stimulation of JNK...
  39. ncbi request reprint [Action of cytotoxic necrotizing factor from E. coli]
    Luce Landraud
    Med Sci (Paris) 19:403-5. 2003
  40. ncbi request reprint Urinary interleukin-8 is elevated in urinary tract infections independently of the causative germs
    Olivier Oregioni
    Laboratoire de Bacteriologie, CHU de Nice, France
    Cytokine 31:415-8. 2005
    ..We suggest that the high negative predictive value of the IL-8 urine assay could be useful to eliminate UTIs in routine screenings...
  41. doi request reprint Isoform-specific interaction of C-RAF with mitochondria
    Antoine Galmiche
    Institut fur Medizinische Strahlenkunde und Zellforschung, University of Wurzburg, Wurzburg, Germany
    J Biol Chem 283:14857-66. 2008
    ....