patrick legembre


Affiliation: Universite de Rennes-1
Location: Rennes, France
Researcher in Fas signaling


  1. Barnhart B, Legembre P, Pietras E, Bubici C, Franzoso G, Peter M. CD95 ligand induces motility and invasiveness of apoptosis-resistant tumor cells. EMBO J. 2004;23:3175-85 pubmed
    ..This could become highly relevant during chemotherapy, which can cause upregulation of CD95 ligand by both tumor and nontumor cells. ..
  2. Khadra N, Bresson Bepoldin L, Penna A, Chaigne Delalande B, Segui B, Levade T, et al. CD95 triggers Orai1-mediated localized Ca2+ entry, regulates recruitment of protein kinase C (PKC) ?2, and prevents death-inducing signaling complex formation. Proc Natl Acad Sci U S A. 2011;108:19072-7 pubmed publisher
    ..We suggest that these localized events provide a time of decision to prevent accidental cell death. ..
  3. Tauzin S, Debure L, Moreau J, Legembre P. CD95-mediated cell signaling in cancer: mutations and post-translational modulations. Cell Mol Life Sci. 2012;69:1261-77 pubmed publisher
    ..The focus of this review is to discuss these apparent contradictions of the known function(s) of CD95. ..
  4. Tauzin S, Chaigne Delalande B, Selva E, Khadra N, Daburon S, Contin Bordes C, et al. The naturally processed CD95L elicits a c-yes/calcium/PI3K-driven cell migration pathway. PLoS Biol. 2011;9:e1001090 pubmed publisher
  5. Pizon M, Rampanarivo H, Tauzin S, Chaigne Delalande B, Daburon S, Castroviejo M, et al. Actin-independent exclusion of CD95 by PI3K/AKT signalling: implications for apoptosis. Eur J Immunol. 2011;41:2368-78 pubmed publisher
    ..Overall, these findings highlight that the oncogenic PI3K/Akt signalling pathway participates in maintaining cells in a type II phenotype by excluding CD95 from lipid rafts. ..
  6. Leon Bollotte L, Subramaniam S, Cauvard O, Plenchette Colas S, Paul C, Godard C, et al. S-nitrosylation of the death receptor fas promotes fas ligand-mediated apoptosis in cancer cells. Gastroenterology. 2011;140:2009-18, 2018.e1-4 pubmed publisher
    ..S-nitrosylation at cysteine 304 promotes redistribution of Fas to lipid rafts, formation of the death-inducing signal complex, and induction of cell death. ..
  7. Chaigne Delalande B, Guidicelli G, Couzi L, Merville P, Mahfouf W, Bouchet S, et al. The immunosuppressor mycophenolic acid kills activated lymphocytes by inducing a nonclassical actin-dependent necrotic signal. J Immunol. 2008;181:7630-8 pubmed
    ..In addition to its medical interest, this study sheds light on a novel and atypical molecular mechanism leading to necrotic cell death. ..
  8. Beneteau M, Pizon M, Chaigne Delalande B, Daburon S, Moreau P, De Giorgi F, et al. Localization of Fas/CD95 into the lipid rafts on down-modulation of the phosphatidylinositol 3-kinase signaling pathway. Mol Cancer Res. 2008;6:604-13 pubmed publisher
    ..These findings indicate that the antitumoral agent edelfosine down-modulates the PI3K signal to sensitize tumor cells to death through the redistribution of Fas into large platform of membrane rafts. ..
  9. Legembre P, Barnhart B, Zheng L, Vijayan S, Straus S, Puck J, et al. Induction of apoptosis and activation of NF-kappaB by CD95 require different signalling thresholds. EMBO Rep. 2004;5:1084-9 pubmed
    ..Mutations in CD95 may eliminate the tumour-suppressive function of CD95, at the same time allowing induction of survival or proliferative pathways, which could contribute to the increased risk for lymphoma seen in ALPS type Ia patients. ..

Detail Information


  1. Chaigne Delalande B, Mahfouf W, Daburon S, Moreau J, Legembre P. CD95 engagement mediates actin-independent and -dependent apoptotic signals. Cell Death Differ. 2009;16:1654-64 pubmed publisher
    ..In conclusion, the addition of APO1-3 on type I cells triggers an actin-dependent apoptotic signal, which is absent or marginal in cells (both types I and II) treated with CD95L. ..
  2. Schembri L, Dalibart R, Tomasello F, Legembre P, Ichas F, De Giorgi F. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007;4:107-8 pubmed
  3. Legembre P, Daburon S, Moreau P, Moreau J, Taupin J. Modulation of Fas-mediated apoptosis by lipid rafts in T lymphocytes. J Immunol. 2006;176:716-20 pubmed
    ..Moreover, our results demonstrate that Fas-induced apoptosis can be controlled at the level of the cytoplasmic membrane. ..
  4. Fouqué A, Jean M, Weghe P, Legembre P. Review of PI3K/mTOR Inhibitors Entering Clinical Trials to Treat Triple Negative Breast Cancers. Recent Pat Anticancer Drug Discov. 2016;11:283-96 pubmed
    ..Drugs inhibiting the PI3K/mTOR signaling pathway may represent tailored anti-tumor agents, paving the way for their clinical application in different tumor types. ..
  5. Poissonnier A, Sanséau D, Le Gallo M, Malleter M, Levoin N, Viel R, et al. CD95-Mediated Calcium Signaling Promotes T Helper 17 Trafficking to Inflamed Organs in Lupus-Prone Mice. Immunity. 2016;45:209-23 pubmed publisher
    ..Therefore, neutralizing the CD95 non-apoptotic signaling pathway could be an attractive therapeutic approach for SLE treatment. ..
  6. Le Gallo M, Legembre P. CD95 Stimulation with CD95L and DISC Analysis. Methods Mol Biol. 2017;1557:11-18 pubmed publisher
    ..The goal of this chapter is to describe a method used to immunoprecipitate CD95 and analyze its associated protein complex in cells stimulated with a cytotoxic CD95L (i.e., Ig-CD95L). ..
  7. Fouqué A, Legembre P. Study of the CD95-Mediated Non-apoptotic Signaling Pathway: PI3K. Methods Mol Biol. 2017;1557:103-110 pubmed publisher
    ..Cancer Res 73(22):6711-6721, 2013; Tauzin et al., PLoS Biol 9(6):e1001090, 2011; Kleber et al., Cancer Cell 13(3):235-248, 2008). This chapter describes how to immunoprecipitate CD95 to characterize MISC involved in PI3K activation. ..
  8. Poissonnier A, Legembre P. Boyden Chamber Assay to Study of Cell Migration Induced by Metalloprotease Cleaved-CD95L. Methods Mol Biol. 2017;1557:117-123 pubmed publisher
    ..PLoS Biol 9:e1001090, 2011). This chapter aims at describing the methodology used to measure the chemoattractive effect of cl-CD95L on human cancer cells and lymphocytes. ..
  9. Thomas M, Legembre P. Proximity Ligation Assay (PLA) to Evaluate DISC and MISC Composition. Methods Mol Biol. 2017;1557:41-48 pubmed publisher
    ..We here describe PLA used to confirm CD95/FADD interaction, a protocol that may serve to highlight other CD95 partners. ..