Jean Jacques Lacapere

Summary

Country: France

Publications

  1. ncbi request reprint Determining membrane protein structures: still a challenge!
    Jean Jacques Lacapere
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Faculte de Medecine X Bichat, Universite Paris 7, BP 416, F 75018, Paris, France
    Trends Biochem Sci 32:259-70. 2007
  2. ncbi request reprint Peripheral-type benzodiazepine receptor: structure and function of a cholesterol-binding protein in steroid and bile acid biosynthesis
    Jean Jacques Lacapere
    Unité INSERM U410, Faculte de Medecine Xavier Bichat, 16 rue Henri Huchard, 75870 Paris Cedex 18, France
    Steroids 68:569-85. 2003
  3. pmc Transconformations of the SERCA1 Ca-ATPase: a normal mode study
    Nathalie Reuter
    U410 INSERM Faculté de médecine Xavier Bichat, Paris Cedex 18, France
    Biophys J 85:2186-97. 2003
  4. pmc Overexpression of translocator protein in inflammatory bowel disease: potential diagnostic and treatment value
    Mariano A Ostuni
    Unité INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Paris 7 Denis Diderot, Paris, France
    Inflamm Bowel Dis 16:1476-87. 2010
  5. ncbi request reprint Identification of residues within human glycoprotein VI involved in the binding to collagen: evidence for the existence of distinct binding sites
    Christelle Lecut
    INSERM, E348, Faculte de Medecine Xavier Bichat, Paris Cedex 18, France
    J Biol Chem 279:52293-9. 2004
  6. ncbi request reprint Peptide agonist docking in the N-terminal ectodomain of a class II G protein-coupled receptor, the VPAC1 receptor. Photoaffinity, NMR, and molecular modeling
    Yossan Var Tan
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, BP 416, F 75018, Paris, the Université Paris 7 Denis Diderot, site Bichat, BP 416, F 75018, Paris
    J Biol Chem 281:12792-8. 2006
  7. ncbi request reprint The human VPAC1 receptor: identification of the N-terminal ectodomain as a major VIP-binding site by photoaffinity labeling and 3D modeling
    Alain Couvineau
    INSERM U773, Faculte de Medecine X Bichat, 16 rue Henri Huchard, 75018 Paris, France
    Ann N Y Acad Sci 1070:205-9. 2006
  8. ncbi request reprint The vasoactive intestinal peptide (VIP) alpha-Helix up to C terminus interacts with the N-terminal ectodomain of the human VIP/Pituitary adenylate cyclase-activating peptide 1 receptor: photoaffinity, molecular modeling, and dynamics
    Emilie Ceraudo
    Institut National de la Santé et de la Recherche Médicale Unité 773, Centre de recherche Biomedicale Bichat Beaujon, CRB3, Faculte de Medecine Xavier Bichat, 75018, Paris, France
    Mol Endocrinol 22:147-55. 2008
  9. ncbi request reprint Effect of translocator protein (18 kDa)-ligand binding on neurotransmitter-induced salivary secretion in rat submandibular glands
    Mariano A Ostuni
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, université Paris VII Denis Diderot, Paris, France
    Biol Cell 100:427-39. 2008
  10. ncbi request reprint Identification of cytoplasmic domains of hVPAC1 receptor required for activation of adenylyl cyclase. Crucial role of two charged amino acids strictly conserved in class II G protein-coupled receptors
    Alain Couvineau
    INSERM U410 Neuroendocrinologie et Biologie Cellulaire Digestives, Institut National de la Sante et de la Recherche Medicale, Faculte de Medecine Xavier Bichat, F 75018 Paris, France
    J Biol Chem 278:24759-66. 2003

Collaborators

Detail Information

Publications25

  1. ncbi request reprint Determining membrane protein structures: still a challenge!
    Jean Jacques Lacapere
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Faculte de Medecine X Bichat, Universite Paris 7, BP 416, F 75018, Paris, France
    Trends Biochem Sci 32:259-70. 2007
    ..Progress in this field will improve knowledge of ligand-induced activation and inhibition of membrane proteins in addition to aiding the design of membrane-protein-targeted drugs...
  2. ncbi request reprint Peripheral-type benzodiazepine receptor: structure and function of a cholesterol-binding protein in steroid and bile acid biosynthesis
    Jean Jacques Lacapere
    Unité INSERM U410, Faculte de Medecine Xavier Bichat, 16 rue Henri Huchard, 75870 Paris Cedex 18, France
    Steroids 68:569-85. 2003
    ..Taking into account the widespread presence of PBR in many tissues, a more general role of PBR in intracellular cholesterol transport and compartmentalization might be considered...
  3. pmc Transconformations of the SERCA1 Ca-ATPase: a normal mode study
    Nathalie Reuter
    U410 INSERM Faculté de médecine Xavier Bichat, Paris Cedex 18, France
    Biophys J 85:2186-97. 2003
    ..Subsequently, we discuss the potential contribution of the remaining low-frequency normal modes to the transconformations occurring within the overall calcium transport cycle...
  4. pmc Overexpression of translocator protein in inflammatory bowel disease: potential diagnostic and treatment value
    Mariano A Ostuni
    Unité INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Paris 7 Denis Diderot, Paris, France
    Inflamm Bowel Dis 16:1476-87. 2010
    ..In addition, we evaluated the potential use of TSPO drug ligands in diagnosing and treating IBD...
  5. ncbi request reprint Identification of residues within human glycoprotein VI involved in the binding to collagen: evidence for the existence of distinct binding sites
    Christelle Lecut
    INSERM, E348, Faculte de Medecine Xavier Bichat, Paris Cedex 18, France
    J Biol Chem 279:52293-9. 2004
    ..Site-directed mutagenesis revealed that valine 34 and leucine 36 are critical for GPVI interaction with collagen and CRP. The loop might thus be part of a collagen/CRP-binding site...
  6. ncbi request reprint Peptide agonist docking in the N-terminal ectodomain of a class II G protein-coupled receptor, the VPAC1 receptor. Photoaffinity, NMR, and molecular modeling
    Yossan Var Tan
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, BP 416, F 75018, Paris, the Université Paris 7 Denis Diderot, site Bichat, BP 416, F 75018, Paris
    J Biol Chem 281:12792-8. 2006
    ..The data provide a structural rationale to the proposed two-step activation mechanism of VPAC receptor and more generally of class II G protein-coupled receptors...
  7. ncbi request reprint The human VPAC1 receptor: identification of the N-terminal ectodomain as a major VIP-binding site by photoaffinity labeling and 3D modeling
    Alain Couvineau
    INSERM U773, Faculte de Medecine X Bichat, 16 rue Henri Huchard, 75018 Paris, France
    Ann N Y Acad Sci 1070:205-9. 2006
    ..A VIP-binding site was identified on the side of the structured core of the N-terminal ectodomain of the receptor...
  8. ncbi request reprint The vasoactive intestinal peptide (VIP) alpha-Helix up to C terminus interacts with the N-terminal ectodomain of the human VIP/Pituitary adenylate cyclase-activating peptide 1 receptor: photoaffinity, molecular modeling, and dynamics
    Emilie Ceraudo
    Institut National de la Santé et de la Recherche Médicale Unité 773, Centre de recherche Biomedicale Bichat Beaujon, CRB3, Faculte de Medecine Xavier Bichat, 75018, Paris, France
    Mol Endocrinol 22:147-55. 2008
    ..These structural studies provide a detailed molecular understanding of the VIP-receptor interaction...
  9. ncbi request reprint Effect of translocator protein (18 kDa)-ligand binding on neurotransmitter-induced salivary secretion in rat submandibular glands
    Mariano A Ostuni
    INSERM, U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, université Paris VII Denis Diderot, Paris, France
    Biol Cell 100:427-39. 2008
    ..In present study, we investigated the localization and function of TSPO in salivary glands...
  10. ncbi request reprint Identification of cytoplasmic domains of hVPAC1 receptor required for activation of adenylyl cyclase. Crucial role of two charged amino acids strictly conserved in class II G protein-coupled receptors
    Alain Couvineau
    INSERM U410 Neuroendocrinologie et Biologie Cellulaire Digestives, Institut National de la Sante et de la Recherche Medicale, Faculte de Medecine Xavier Bichat, F 75018 Paris, France
    J Biol Chem 278:24759-66. 2003
    ..Because these charged residues are absolutely conserved in class II receptors for peptides, which are all mediating AC activation, they may play a general role in coupling of class II receptors with the Gs protein...
  11. ncbi request reprint Translocator protein (18 kDa) ligand PK 11195 induces transient mitochondrial Ca2+ release leading to transepithelial Cl- secretion in HT-29 human colon cancer cells
    Mariano A Ostuni
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Paris 7 Denis Diderot, F 75018 Paris, France
    Biol Cell 99:639-47. 2007
    ..TSPO has been reported to be over-expressed in human colorectal tumours and cancer cell lines, but its function is not well characterized...
  12. ncbi request reprint Fluorometric studies of ligand-induced conformational changes of CD38
    Jean Jacques Lacapere
    INSERM U410, Faculte de Medecine Xavier Bichat, 16 rue Henri Huchard, 75018 Paris, France
    Biochim Biophys Acta 1652:17-26. 2003
    ..Together, these data demonstrate that CD38 undergoes a reversible conformational change after substrate binding, and suggest a mechanism by which this change could alter interactions with different cell-surface partners...
  13. ncbi request reprint Chimeric Fc receptors identify ligand binding regions in human glycoprotein VI
    Bénédicte Dumont
    Institut National de la Sante et de la Recherche Medicale, INSERM, U698, Universite Paris 7, Hopital Bichat, France
    J Mol Biol 361:877-87. 2006
    ....
  14. doi request reprint Genetic variation at KIT locus may predispose to melanoma
    Agnes Bourillon
    Département de génétique, Hopital Bichat Claude Bernard, APHP, Paris, France
    Pigment Cell Melanoma Res 26:88-96. 2013
    ..Our data strongly suggest that rare KIT substitutions predispose to melanoma and that common variants at KIT locus may also impact nevus count and melanoma risk...
  15. ncbi request reprint Bacterial overexpressed membrane proteins: an example: the TSPO
    Jean Claude Robert
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Faculte de Medecine X, Bichat, Universite Paris 7, Paris, France
    Methods Mol Biol 654:29-45. 2010
    ..Small-scale production at lower cost for isotopically labelled recombinant TSPO and/or detergent is also presented...
  16. ncbi request reprint Characterization of membrane protein preparations: measurement of detergent content and ligand binding after proteoliposomes reconstitution
    Mariano A Ostuni
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Faculte de Medecine X, Bichat, Universite Paris 7, Paris, France
    Methods Mol Biol 654:3-18. 2010
    ..These protocols are useful to characterize functionality and detergent content of membrane protein, both key factors for further structural studies...
  17. ncbi request reprint Electron microscope tomography of native membranes
    Gabriel Péranzi
    INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Faculte de Medecine X, Bichat, Universite Paris 7, Paris, France
    Methods Mol Biol 654:221-35. 2010
    ..In pathological conditions, other mitochondrial morphologies have been described, for instance, vesicular structures for inner boundary membrane have been observed...
  18. ncbi request reprint Characterization of the new photoaffinity probe (Bz2-K24)-VIP
    Yossan Var Tan
    INSERM U773, Faculté de médecine Bichat, 16 rue Henri Huchard, 75018 Paris, France
    Ann N Y Acad Sci 1070:575-80. 2006
    ..The N-terminal ectodomain of the VPAC1 receptor is therefore an affinity trap for the central part of VIP, at least between residues 6 and 24...
  19. ncbi request reprint [A new role for peripheral benzodiazepine receptor?]
    Mariano A Ostuni
    Inserm U 683, Faculte de Medecine Xavier Bichat, 16, rue Henri Huchard, 75870 Paris Cedex 18, France
    Med Sci (Paris) 21:240-2. 2005
  20. doi request reprint Mouse TSPO in a lipid environment interacting with a functionalized monolayer
    David Teboul
    Universite Paris, Denis Diderot, Paris, France
    Biochim Biophys Acta 1818:2791-800. 2012
    ....
  21. ncbi request reprint Distribution, pharmacological characterization and function of the 18 kDa translocator protein in rat small intestine
    Mariano Anibal Ostuni
    Unité INSERM U773, Centre de Recherche Biomedicale Bichat Beaujon CRB3, Universite Paris 7 Denis Diderot, BP 416, F 75018 Paris, France
    Biol Cell 101:573-86. 2009
    ..Here, we investigate the localization and functions of TSPO in the small intestine...
  22. doi request reprint Subtle conformational changes between CX3CR1 genetic variants as revealed by resonance energy transfer assays
    Kévin Darbandi-Tehrani
    Laboratoire Immunité et Infection, INSERM UMR S 945, Paris, France
    FASEB J 24:4585-98. 2010
    ....
  23. ncbi request reprint Association between endothelin receptor B nonsynonymous variants and melanoma risk
    Nadem Soufir
    Laboratoire de Biochimie hormonale et génétique, Hopital Bichat Claude Bernard, AP HP, Faculte de Medecine, Paris VII, Paris, France
    J Natl Cancer Inst 97:1297-301. 2005
    ..9, 95% CI = 1.34 to 296.2; P = .03). Our data strongly suggest that EDNRB is involved in predisposition for two different multigenic disorders, HSCR and melanoma...
  24. ncbi request reprint Design and humanization of a murine scFv that blocks human platelet glycoprotein VI in vitro
    Julien Muzard
    Museum national d histoire naturelle, CNRS FRE 3206, Paris, France
    FEBS J 276:4207-22. 2009
    ..This is a simple, efficient and straightforward method that could also be used for humanizing other antibodies...
  25. doi request reprint Production and purification of large quantities of the functional N-terminal ectodomain of human VPAC1 receptor
    Alain Couvineau
    INSERM U773, Institut National de la Sante et de la Recherche Medicale, Centre de recherche Biomedicale Bichat Beaujon, CRB3, Faculte de Medecine Xavier Bichat, 75018, Paris, France
    J Mol Neurosci 36:249-53. 2008
    ..About 5-10 mg of functional purified protein/liter of bacterial culture is currently produced. This is a crucial step to determine the structure of functional human VPAC1 receptor N-ted by nuclear magnetic resonance...