Andrey S Klymchenko

Summary

Country: France

Publications

  1. ncbi request reprint Fluorescent probe based on intramolecular proton transfer for fast ratiometric measurement of cellular transmembrane potential
    Andrey S Klymchenko
    UMR 7175 CNRS, Département Pharmacologie et Physicochimie, Université Louis Pasteur Strasbourg 1, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    J Phys Chem B 110:13624-32. 2006
  2. doi request reprint Excited-state intramolecular proton transfer distinguishes microenvironments in single- and double-stranded DNA
    Andrey S Klymchenko
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401, Illkirch, France
    J Phys Chem B 112:12050-5. 2008
  3. doi request reprint Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone
    Andrey S Klymchenko
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Biochim Biophys Acta 1788:495-9. 2009
  4. doi request reprint 2D analogues of the inverted hexagonal phase self-assembled from 4,6-dialkoxylated isophthalic acids at solid-liquid interfaces
    Andrey S Klymchenko
    Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
    Nanoscale 2:1773-80. 2010
  5. ncbi request reprint Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes
    Vasyl V Shynkar
    Laboratoire de pharmacologie et physicochimie des interactions cellulaires et moléculaires, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Biochim Biophys Acta 1712:128-36. 2005
  6. doi request reprint Detection of apoptosis through the lipid order of the outer plasma membrane leaflet
    Zeinab Darwich
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Biochim Biophys Acta 1818:3048-54. 2012
  7. doi request reprint Lactose-ornithine bolaamphiphiles for efficient gene delivery in vitro
    Namrata Jain
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Int J Pharm 423:392-400. 2012
  8. ncbi request reprint Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects
    Gora M'Baye
    Laboratoire de Pharmacologie et Physicochimie des Interactions Moléculaires et Cellulaires, Equipe Photophysique des Interactions Biomoléculaires, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, BP 60024, 67401, Illkirch, France
    J Fluoresc 16:35-42. 2006
  9. doi request reprint Fluorescent environment-sensitive dyes as reporters of biomolecular interactions
    Andrey S Klymchenko
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
    Prog Mol Biol Transl Sci 113:35-58. 2013
  10. ncbi request reprint Liquid ordered phase in cell membranes evidenced by a hydration-sensitive probe: effects of cholesterol depletion and apoptosis
    Sule Oncul
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
    Biochim Biophys Acta 1798:1436-43. 2010

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Fluorescent probe based on intramolecular proton transfer for fast ratiometric measurement of cellular transmembrane potential
    Andrey S Klymchenko
    UMR 7175 CNRS, Département Pharmacologie et Physicochimie, Université Louis Pasteur Strasbourg 1, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    J Phys Chem B 110:13624-32. 2006
    ..Moreover, the new design principles proposed in the present work should allow further improvement of the probe sensitivity...
  2. doi request reprint Excited-state intramolecular proton transfer distinguishes microenvironments in single- and double-stranded DNA
    Andrey S Klymchenko
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401, Illkirch, France
    J Phys Chem B 112:12050-5. 2008
    ..Consequently, ESIPT-based dyes are promising for monitoring the interaction of polycationic molecules with DNA and probing the microenvironment of their DNA binding sites...
  3. doi request reprint Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavone
    Andrey S Klymchenko
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Biochim Biophys Acta 1788:495-9. 2009
    ....
  4. doi request reprint 2D analogues of the inverted hexagonal phase self-assembled from 4,6-dialkoxylated isophthalic acids at solid-liquid interfaces
    Andrey S Klymchenko
    Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
    Nanoscale 2:1773-80. 2010
    ..Moreover, the substrate potential at the Au(111)-water interface can tune the 2D molecular arrangement...
  5. ncbi request reprint Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes
    Vasyl V Shynkar
    Laboratoire de pharmacologie et physicochimie des interactions cellulaires et moléculaires, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Biochim Biophys Acta 1712:128-36. 2005
    ..Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced...
  6. doi request reprint Detection of apoptosis through the lipid order of the outer plasma membrane leaflet
    Zeinab Darwich
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Biochim Biophys Acta 1818:3048-54. 2012
    ..Thus, for the first time, an environment-sensitive probe, sensitive to lipid order, is shown to detect apoptosis, suggesting a new concept in apoptosis sensing...
  7. doi request reprint Lactose-ornithine bolaamphiphiles for efficient gene delivery in vitro
    Namrata Jain
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Int J Pharm 423:392-400. 2012
    ..Fluorescence imaging showed that the bolaplexes bind rapidly to cell surface and internalize mainly through endocytosis. This work suggests a new type of efficient nonviral vectors based on bolaamphiphiles...
  8. ncbi request reprint Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects
    Gora M'Baye
    Laboratoire de Pharmacologie et Physicochimie des Interactions Moléculaires et Cellulaires, Equipe Photophysique des Interactions Biomoléculaires, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, BP 60024, 67401, Illkirch, France
    J Fluoresc 16:35-42. 2006
    ....
  9. doi request reprint Fluorescent environment-sensitive dyes as reporters of biomolecular interactions
    Andrey S Klymchenko
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
    Prog Mol Biol Transl Sci 113:35-58. 2013
    ..It is shown that solvatochromic dyes are powerful tools for monitoring conformation changes of proteins and their interactions with nucleic acids, proteins, and lipid membranes...
  10. ncbi request reprint Liquid ordered phase in cell membranes evidenced by a hydration-sensitive probe: effects of cholesterol depletion and apoptosis
    Sule Oncul
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
    Biochim Biophys Acta 1798:1436-43. 2010
    ..Our data suggest a new methodology for evaluating the Lo phase in membranes of living cells...
  11. ncbi request reprint Fluorescent dyes undergoing intramolecular proton transfer with improved sensitivity to surface charge in lipid bilayers
    Andrey S Klymchenko
    Département de Pharmacologie et Physicochimie, Photophysique des Interactions Biomoleculaires, UMR 7175, Institut Gilbert Laustriat, 74 route du Rhin, Université Louis Pasteur Strasbourg I, BP 60024, 67401, Illkirch, France
    Photochem Photobiol Sci 6:71-6. 2007
    ..As a consequence, the new dyes appear as prospective fluorophores for the development of new fluorescent probes for biomembranes...
  12. ncbi request reprint Monitoring membrane properties and apoptosis using membrane probes of the 3-hydroxyflavone family
    Zeinab Darwich
    Faculté de Parmacie, Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Illkirch, France
    Methods Mol Biol 1076:419-30. 2014
    ..In the present work, we will describe the protocols used with these probes in order to monitor the physicochemical properties of lipid membrane models and cell plasma membranes and to detect apoptosis. ..
  13. pmc Liquid ordered and gel phases of lipid bilayers: fluorescent probes reveal close fluidity but different hydration
    Gora M'Baye
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Biophys J 95:1217-25. 2008
    ..In this study, the liquid ordered (raft) and gel phases are for the first time clearly distinguished by their strong difference in hydration...
  14. ncbi request reprint Simultaneous probing of hydration and polarity of lipid bilayers with 3-hydroxyflavone fluorescent dyes
    Andrey S Klymchenko
    Laboratoire de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, BP 60024, 67401 Illkirch, France
    Biochim Biophys Acta 1665:6-19. 2004
    ..Furthermore, no correlation was found between dipole potential and the hydration of the bilayers...
  15. ncbi request reprint Modulation of excited-state intramolecular proton transfer by viscosity in protic media
    Dmytro A Yushchenko
    Photophysique des Interactions Biomoleculaires, UMR 7175 LC1 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    J Phys Chem A 111:10435-8. 2007
    ..The present results suggest a new physical principle for the fluorescence ratiometric measurement of local viscosity...
  16. ncbi request reprint Fluorescent biomembrane probe for ratiometric detection of apoptosis
    Vasyl V Shynkar
    Photophysique des interactions moleculaires, UMR 7175 CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, Strasbourg 1, 74 route du Rhin, 67401 Illkirch Cedex, France
    J Am Chem Soc 129:2187-93. 2007
    ....
  17. doi request reprint Excited state proton transfer and solvent relaxation of a 3-hydroxyflavone probe in lipid bilayers
    Ranjan Das
    Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    J Phys Chem B 112:11929-35. 2008
    ....
  18. doi request reprint New unsymmetrical bolaamphiphiles: synthesis, assembly with DNA, and application for gene delivery
    Namrata Jain
    UMR CNRS, Université de Strasbourg, Illkirch, France
    Bioconjug Chem 21:2110-8. 2010
    ..We believe that further optimization of polar head groups and a hydrophobic spacer in the bolas will lead to vectors with controlled small size and high transfection efficiency...
  19. doi request reprint Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranes
    Oleksandr A Kucherak
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    J Am Chem Soc 132:4907-16. 2010
    ..The attractive photophysical and switching properties of NR12S, together with its selective outer leaflet staining and sensitivity to cholesterol and lipid order, make it a new powerful tool for studying model and cell membranes...
  20. doi request reprint Unusually slow proton transfer dynamics of a 3-hydroxychromone dye in protic solvents
    Ranjan Das
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 du CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401, Illkirch, France
    Photochem Photobiol Sci 8:1583-9. 2009
    ..These data provide a physical background for recent applications of this dye as a fluorescence probe of the microenvironment in biomolecules...
  21. doi request reprint Multiparametric probing of microenvironment with solvatochromic fluorescent dyes
    Andrey S Klymchenko
    Lab de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
    Methods Enzymol 450:37-58. 2008
    ..We believe that the methodology of multiparametric probing will extend the capabilities of fluorescent probes as the tools in biomolecular and cellular research...
  22. doi request reprint Two-color fluorescent l-amino acid mimic of tryptophan for probing peptide-nucleic acid complexes
    Aleksandr V Strizhak
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 67401 Illkirch, France
    Bioconjug Chem 23:2434-43. 2012
    ....
  23. doi request reprint Dipolar 3-methoxychromones as bright and highly solvatochromic fluorescent dyes
    Oleksandr A Kucherak
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Phys Chem Chem Phys 14:2292-300. 2012
    ..This new class of fluorescent dyes may serve as attractive building blocks for future molecular sensors utilizing environment-sensitive fluorophores...
  24. doi request reprint Local environment perturbations in alpha1-antitrypsin monitored by a ratiometric fluorescent label
    Christian Boudier
    Laboratoire de Biophotonique et Pharmacologie, UMR CNRS 7213, Faculte de Pharmacie, Universite de Strasbourg, 67401 Illkirch Cedex, France
    Photochem Photobiol Sci 8:814-21. 2009
    ..These results present a convenient fluorescence ratiometric approach based on the BMFC label for studies of protein conformational changes...
  25. doi request reprint Ab initio study of the solvent H-bonding effect on ESIPT reaction and electronic transitions of 3-hydroxychromone derivatives
    Cyril A Kenfack
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 du CNRS, Faculte de Pharmacie, Universite de Strasbourg, 74, route du Rhin, 67401 Illkirch Cedex, France
    Phys Chem Chem Phys 14:8910-8. 2012
    ..Therefore, the ESIPT reaction in DMAF is controlled by the relative energies of the excited states (thermodynamic control), while in FHC the ESIPT is controlled probably by the energetic barrier (kinetic control)...
  26. doi request reprint A FRET-based probe with a chemically deactivatable quencher
    Geoffray Leriche
    Laboratory of Functional Chemo Systems UMR 7199, 74 route du Rhin, 67401, Illkirch, France
    Chem Commun (Camb) 48:3224-6. 2012
    ..This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments...
  27. ncbi request reprint Collective fluorescence switching of counterion-assembled dyes in polymer nanoparticles
    Andreas Reisch
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch, France
    Nat Commun 5:4089. 2014
    ..The counterion-based concept opens the way to a new class of nanomaterials for sensing, imaging and light harvesting. ..
  28. doi request reprint Dual-fluorescence l-amino acid reports insertion and orientation of melittin peptide in cell membranes
    Viktoriia Y Postupalenko
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Bioconjug Chem 24:1998-2007. 2013
    ..The developed amino acid and the proposed methodology will be of interest to study other membrane peptides. ..
  29. doi request reprint Virus-sized DNA nanoparticles for gene delivery based on micelles of cationic calixarenes
    Roman V Rodik
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
    Chemistry 17:5526-38. 2011
    ..Moreover, all cationic calixarenes studied showed low cytotoxicity. Thus, this work presents a two-step hierarchical assembly of small DNA nanoparticles for gene delivery based on amphiphilic cone-shaped cationic calixarenes...
  30. ncbi request reprint Resolution of Cys and Lys labeling of alpha-crystallin with site-sensitive fluorescent 3-hydroxyflavone dye
    Andrey S Klymchenko
    Laboratoire de Pharmacologie et Physicochimie des intercations cellulaires et moléculaires, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401, Illkirch, France
    Anal Biochem 329:43-57. 2004
    ....
  31. pmc Sensing peptide-oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid protein
    Volodymyr V Shvadchak
    Laboratoire de Biophotonique et Pharmacologie, Faculte de Pharmacie, UMR 7213 du CNRS, Universite de Strasbourg, 67401 Illkirch, France
    Nucleic Acids Res 37:e25. 2009
    ..The described methodology could become a new universal tool for investigating peptide-ODN interactions...
  32. ncbi request reprint Steric control of the excited-state intramolecular proton transfer in 3-hydroxyquinolones: steady-state and time-resolved fluorescence study
    Dmytro A Yushchenko
    Department of Chemistry, Kyiv National Taras Shevchenko University, 01033 Kyiv, Ukraine
    J Phys Chem A 111:8986-92. 2007
    ..This conclusion provides a new understanding of the ESIPT process in 3-hydroxyquinolones and related systems and suggests new possibilities for the design of ESIPT based molecular sensors and switchers...
  33. doi request reprint The structure of HBsAg particles is not modified upon their adsorption on aluminium hydroxide gel
    Vanille J Greiner
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
    Vaccine 30:5240-5. 2012
    ..Taken together, our data show that adsorption on Al gel does not affect the structure of the HBsAg particles...
  34. doi request reprint Red fluorescent turn-on ligands for imaging and quantifying G protein-coupled receptors in living cells
    Iuliia A Karpenko
    Laboratoire d Innovation Thérapeutique UMR 7200 UdS CNRS, LabEx Medalis, Faculte de Pharmacie, Universite de Strasbourg, 74 route du Rhin, 67401 Illkirch Cedex France
    Chembiochem 15:359-63. 2014
    ..The new ligand was successfully applied for background-free imaging and quantification of oxytocin receptors in living cells...
  35. ncbi request reprint Counterion-enhanced cyanine dye loading into lipid nano-droplets for single-particle tracking in zebrafish
    Vasyl N Kilin
    Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch, France
    Biomaterials 35:4950-7. 2014
    ..The concept of counterion-based dye loading provides a new effective route to ultra-bright lipid nanoparticles, which enables tracking single particles in live animals, a new dimension of in vivo imaging...
  36. pmc Bimodal distribution and fluorescence response of environment-sensitive probes in lipid bilayers
    Andrey S Klymchenko
    Laboratoire de Pharmacologie et Physicochimie, Unité Mixte de Recherche 7034 du Centre National de la Recherche Scientifique, Faculte de Pharmacie, Universite Louis Pasteur, Illkirch, France
    Biophys J 86:2929-41. 2004
    ..in lipid bilayers allows us to suggest that the bimodal distribution in the lipid bilayer is probably a general feature of low-polar molecules with polar groups capable of H-bonding interactions...