Research Topics
| Andrey S KlymchenkoSummaryCountry: France Publications
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Publications
Fluorescent probe based on intramolecular proton transfer for fast ratiometric measurement of cellular transmembrane potentialAndrey S Klymchenko
UMR 7175 CNRS, Département Pharmacologie et Physicochimie, Université Louis Pasteur Strasbourg 1, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
J Phys Chem B 110:13624-32. 2006..Moreover, the new design principles proposed in the present work should allow further improvement of the probe sensitivity...
Excited-state intramolecular proton transfer distinguishes microenvironments in single- and double-stranded DNAAndrey S Klymchenko
Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401, Illkirch, France
J Phys Chem B 112:12050-5. 2008..Consequently, ESIPT-based dyes are promising for monitoring the interaction of polycationic molecules with DNA and probing the microenvironment of their DNA binding sites...- 2D analogues of the inverted hexagonal phase self-assembled from 4,6-dialkoxylated isophthalic acids at solid-liquid interfacesAndrey S Klymchenko
Department of Chemistry, Katholieke Universiteit Leuven, Leuven, Belgium
Nanoscale 2:1773-80. 2010..Moreover, the substrate potential at the Au(111)-water interface can tune the 2D molecular arrangement... - Visualization of lipid domains in giant unilamellar vesicles using an environment-sensitive membrane probe based on 3-hydroxyflavoneAndrey S Klymchenko
Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
Biochim Biophys Acta 1788:495-9. 2009.... - Detection of apoptosis through the lipid order of the outer plasma membrane leafletZeinab Darwich
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
Biochim Biophys Acta 1818:3048-54. 2012..Thus, for the first time, an environment-sensitive probe, sensitive to lipid order, is shown to detect apoptosis, suggesting a new concept in apoptosis sensing... - Lactose-ornithine bolaamphiphiles for efficient gene delivery in vitroNamrata Jain
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
Int J Pharm 423:392-400. 2012..Fluorescence imaging showed that the bolaplexes bind rapidly to cell surface and internalize mainly through endocytosis. This work suggests a new type of efficient nonviral vectors based on bolaamphiphiles... - Two-color fluorescent probes for imaging the dipole potential of cell plasma membranesVasyl V Shynkar
, UMR 7034 du CNRS, , , 67401 Illkirch, France
Biochim Biophys Acta 1712:128-36. 2005..Using this new tool, a heterogeneous distribution of this potential within the membrane was evidenced... - Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effectsVasyl V Shynkar
, , UMR 7034 du CNRS, , , BP 60024, 67401, Illkirch, France
J Fluoresc 16:35-42. 2006.... - Fluorescent dyes undergoing intramolecular proton transfer with improved sensitivity to surface charge in lipid bilayersAndrey S Klymchenko
Département de Pharmacologie et Physicochimie, Photophysique des Interactions Biomoleculaires, UMR 7175, Institut Gilbert Laustriat, 74 route du Rhin, Université Louis Pasteur Strasbourg I, BP 60024, 67401, Illkirch, France
Photochem Photobiol Sci 6:71-6. 2007..As a consequence, the new dyes appear as prospective fluorophores for the development of new fluorescent probes for biomembranes... - Liquid ordered phase in cell membranes evidenced by a hydration-sensitive probe: effects of cholesterol depletion and apoptosisSule Oncul
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
Biochim Biophys Acta 1798:1436-43. 2010..Our data suggest a new methodology for evaluating the Lo phase in membranes of living cells... - Modulation of excited-state intramolecular proton transfer by viscosity in protic mediaDmytro A Yushchenko
Photophysique des Interactions Biomoleculaires, UMR 7175 LC1 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
J Phys Chem A 111:10435-8. 2007..The present results suggest a new physical principle for the fluorescence ratiometric measurement of local viscosity... - Simultaneous probing of hydration and polarity of lipid bilayers with 3-hydroxyflavone fluorescent dyesAndrey S Klymchenko
Laboratoire de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, BP 60024, 67401 Illkirch, France
Biochim Biophys Acta 1665:6-19. 2004..Furthermore, no correlation was found between dipole potential and the hydration of the bilayers... - Switchable nile red-based probe for cholesterol and lipid order at the outer leaflet of biomembranesOleksandr A Kucherak
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
J Am Chem Soc 132:4907-16. 2010..The attractive photophysical and switching properties of NR12S, together with its selective outer leaflet staining and sensitivity to cholesterol and lipid order, make it a new powerful tool for studying model and cell membranes... - New unsymmetrical bolaamphiphiles: synthesis, assembly with DNA, and application for gene deliveryNamrata Jain
UMR CNRS, Université de Strasbourg, Illkirch, France
Bioconjug Chem 21:2110-8. 2010..We believe that further optimization of polar head groups and a hydrophobic spacer in the bolas will lead to vectors with controlled small size and high transfection efficiency... - Unusually slow proton transfer dynamics of a 3-hydroxychromone dye in protic solventsRanjan Das
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 du CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401, Illkirch, France
Photochem Photobiol Sci 8:1583-9. 2009..These data provide a physical background for recent applications of this dye as a fluorescence probe of the microenvironment in biomolecules... - Multiparametric probing of microenvironment with solvatochromic fluorescent dyesAndrey S Klymchenko
Lab de Pharmacologie et Physicochimie, UMR 7034 du CNRS, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
Methods Enzymol 450:37-58. 2008..We believe that the methodology of multiparametric probing will extend the capabilities of fluorescent probes as the tools in biomolecular and cellular research... - Excited state proton transfer and solvent relaxation of a 3-hydroxyflavone probe in lipid bilayersRanjan Das
Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
J Phys Chem B 112:11929-35. 2008.... 
Liquid ordered and gel phases of lipid bilayers: fluorescent probes reveal close fluidity but different hydrationGora M'Baye
Photophysique des Interactions Biomoleculaires, UMR 7175 du CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, 67401 Illkirch, France
Biophys J 95:1217-25. 2008..In this study, the liquid ordered (raft) and gel phases are for the first time clearly distinguished by their strong difference in hydration...- Fluorescent biomembrane probe for ratiometric detection of apoptosisVasyl V Shynkar
Photophysique des interactions moleculaires, UMR 7175 CNRS, Institut Gilbert Laustriat, Faculte de Pharmacie, Universite Louis Pasteur, Strasbourg 1, 74 route du Rhin, 67401 Illkirch Cedex, France
J Am Chem Soc 129:2187-93. 2007.... - Dipolar 3-methoxychromones as bright and highly solvatochromic fluorescent dyesOleksandr A Kucherak
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
Phys Chem Chem Phys 14:2292-300. 2012..This new class of fluorescent dyes may serve as attractive building blocks for future molecular sensors utilizing environment-sensitive fluorophores... - Local environment perturbations in alpha1-antitrypsin monitored by a ratiometric fluorescent labelChristian Boudier
Laboratoire de Biophotonique et Pharmacologie, UMR CNRS 7213, Faculte de Pharmacie, Universite de Strasbourg, 67401 Illkirch Cedex, France
Photochem Photobiol Sci 8:814-21. 2009..These results present a convenient fluorescence ratiometric approach based on the BMFC label for studies of protein conformational changes... - Ab initio study of the solvent H-bonding effect on ESIPT reaction and electronic transitions of 3-hydroxychromone derivativesCyril A Kenfack
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 du CNRS, Faculte de Pharmacie, Universite de Strasbourg, 74, route du Rhin, 67401 Illkirch Cedex, France
Phys Chem Chem Phys 14:8910-8. 2012..Therefore, the ESIPT reaction in DMAF is controlled by the relative energies of the excited states (thermodynamic control), while in FHC the ESIPT is controlled probably by the energetic barrier (kinetic control)... - A FRET-based probe with a chemically deactivatable quencherGeoffray Leriche
Laboratory of Functional Chemo Systems UMR 7199, 74 route du Rhin, 67401, Illkirch, France
Chem Commun (Camb) 48:3224-6. 2012..This concept allowed us to quantify the caspase-3 cleavage activity in solution and to reveal unreacted probes in cell experiments... - Sensing peptide-oligonucleotide interactions by a two-color fluorescence label: application to the HIV-1 nucleocapsid proteinVolodymyr V Shvadchak
Laboratoire de Biophotonique et Pharmacologie, Faculte de Pharmacie, UMR 7213 du CNRS, Universite de Strasbourg, 67401 Illkirch, France
Nucleic Acids Res 37:e25. 2009..The described methodology could become a new universal tool for investigating peptide-ODN interactions... - Resolution of Cys and Lys labeling of alpha-crystallin with site-sensitive fluorescent 3-hydroxyflavone dyeAndrey S Klymchenko
, UMR 7034 du CNRS, , , 67401, Illkirch, France
Anal Biochem 329:43-57. 2004.... - Virus-sized DNA nanoparticles for gene delivery based on micelles of cationic calixarenesRoman V Rodik
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74, route du Rhin, 67401 Illkirch Cedex, France
Chemistry 17:5526-38. 2011..Moreover, all cationic calixarenes studied showed low cytotoxicity. Thus, this work presents a two-step hierarchical assembly of small DNA nanoparticles for gene delivery based on amphiphilic cone-shaped cationic calixarenes... - Steric control of the excited-state intramolecular proton transfer in 3-hydroxyquinolones: steady-state and time-resolved fluorescence studyDmytro A Yushchenko
Department of Chemistry, Kyiv National Taras Shevchenko University, 01033 Kyiv, Ukraine
J Phys Chem A 111:8986-92. 2007..This conclusion provides a new understanding of the ESIPT process in 3-hydroxyquinolones and related systems and suggests new possibilities for the design of ESIPT based molecular sensors and switchers... - The structure of HBsAg particles is not modified upon their adsorption on aluminium hydroxide gelVanille J Greiner
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, 67401 Illkirch, France
Vaccine 30:5240-5. 2012..Taken together, our data show that adsorption on Al gel does not affect the structure of the HBsAg particles... - Two-color fluorescent l-amino acid mimic of tryptophan for probing peptide-nucleic acid complexesAleksandr V Strizhak
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, 67401 Illkirch, France
Bioconjug Chem 23:2434-43. 2012.... - Fluorescent environment-sensitive dyes as reporters of biomolecular interactionsAndrey S Klymchenko
Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Universite de Strasbourg, Faculte de Pharmacie, Illkirch Cedex, France
Prog Mol Biol Transl Sci 113:35-58. 2013..It is shown that solvatochromic dyes are powerful tools for monitoring conformation changes of proteins and their interactions with nucleic acids, proteins, and lipid membranes... - Bimodal distribution and fluorescence response of environment-sensitive probes in lipid bilayersAndrey S Klymchenko
Laboratoire de Pharmacologie et Physicochimie, Unité Mixte de Recherche 7034 du Centre National de la Recherche Scientifique, Faculte de Pharmacie, Universite Louis Pasteur, Illkirch, France
Biophys J 86:2929-41. 2004..in lipid bilayers allows us to suggest that the bimodal distribution in the lipid bilayer is probably a general feature of low-polar molecules with polar groups capable of H-bonding interactions...