A Kichler

Summary

Country: France

Publications

  1. pmc Efficient DNA transfection mediated by the C-terminal domain of human immunodeficiency virus type 1 viral protein R
    A Kichler
    Genethon III, CNRS URA 1923, Evry, France
    J Virol 74:5424-31. 2000
  2. ncbi request reprint Dilution of reporter gene with stuffer DNA does not alter the transfection efficiency of polyethylenimines
    Antoine Kichler
    Genethon UMR 8115 CNRS, 1bis rue de l Internationale, BP 60, F 91002 Evry, France
    J Gene Med 7:1459-67. 2005
  3. ncbi request reprint The antibiotic and DNA-transfecting peptide LAH4 selectively associates with, and disorders, anionic lipids in mixed membranes
    A James Mason
    Faculte de Chimie, University Louis Pasteur CNRS UMR717 LC3, Institut Le Bel, Strasbourg, France
    FASEB J 20:320-2. 2006
  4. ncbi request reprint Optimising histidine rich peptides for efficient DNA delivery in the presence of serum
    A James Mason
    Faculte de Chimie, Université Louis Pasteur CNRS LC3 UMR7177, Institut Le Bel, 4 rue Blaise Pascal, F 67070 Strasbourg, France
    J Control Release 118:95-104. 2007
  5. pmc A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair
    Xavier Leclerc
    Genethon FRE3087 CNRS Université d Evry Val d Essonne, 1 rue de l Internationale, Département de Recherche Exploratoire, BP 60, 91002 Evry Cedex, France
    BMC Biotechnol 9:35. 2009
  6. ncbi request reprint Characterization of the gene transfer process mediated by histidine-rich peptides
    Antoine Kichler
    Genethon CNRS UMR 8115, 1bis rue de l Internationale, BP60, 91002 Evry, France
    J Mol Med (Berl) 85:191-201. 2007
  7. ncbi request reprint Cationic amphipathic histidine-rich peptides for gene delivery
    Antoine Kichler
    Genethon UMR 8115, 1 rue de l Internationale, F 91002 Evry, France
    Biochim Biophys Acta 1758:301-7. 2006
  8. ncbi request reprint Cationic steroid antibiotics demonstrate DNA delivery properties
    Antoine Kichler
    Genethon UMR 8115 CNRS, 1bis rue de l Internationale, BP 60, F 91002 Evry, France
    J Control Release 107:174-82. 2005
  9. ncbi request reprint Gene transfer with modified polyethylenimines
    Antoine Kichler
    Genethon CNRS UMR 8115, 1 rue de l Internationale, F 91002 Evry, France
    J Gene Med 6:S3-10. 2004
  10. pmc Histidine-rich amphipathic peptide antibiotics promote efficient delivery of DNA into mammalian cells
    Antoine Kichler
    Généthon III Centre National de la Recherche Scientifique Unité de Recherche Associée 1923, 1 rue de l Internationale, F 91002 Evry, France
    Proc Natl Acad Sci U S A 100:1564-8. 2003

Collaborators

Detail Information

Publications25

  1. pmc Efficient DNA transfection mediated by the C-terminal domain of human immunodeficiency virus type 1 viral protein R
    A Kichler
    Genethon III, CNRS URA 1923, Evry, France
    J Virol 74:5424-31. 2000
    ..Vpr(52-96)-DNA complexes were able to reach the cell nucleus through a pH-independent mechanism. These observations possibly identify an alternate pathway for DNA transfection...
  2. ncbi request reprint Dilution of reporter gene with stuffer DNA does not alter the transfection efficiency of polyethylenimines
    Antoine Kichler
    Genethon UMR 8115 CNRS, 1bis rue de l Internationale, BP 60, F 91002 Evry, France
    J Gene Med 7:1459-67. 2005
    ..Here, we investigate whether it is possible to reduce the amount of plasmid DNA while keeping a high transfection efficiency...
  3. ncbi request reprint The antibiotic and DNA-transfecting peptide LAH4 selectively associates with, and disorders, anionic lipids in mixed membranes
    A James Mason
    Faculte de Chimie, University Louis Pasteur CNRS UMR717 LC3, Institut Le Bel, Strasbourg, France
    FASEB J 20:320-2. 2006
    ..This work suggests a mechanism of selective destabilization by LAH4 of anionic lipids in the membranes of cells during transfection with implications for nucleic acid delivery in vivo...
  4. ncbi request reprint Optimising histidine rich peptides for efficient DNA delivery in the presence of serum
    A James Mason
    Faculte de Chimie, Université Louis Pasteur CNRS LC3 UMR7177, Institut Le Bel, 4 rue Blaise Pascal, F 67070 Strasbourg, France
    J Control Release 118:95-104. 2007
    ..The detailed understanding of these two key stages in the transfection process shows that LAH4-L1 and its derivatives are likely to be highly efficient and robust vectors for a range of applications...
  5. pmc A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair
    Xavier Leclerc
    Genethon FRE3087 CNRS Université d Evry Val d Essonne, 1 rue de l Internationale, Département de Recherche Exploratoire, BP 60, 91002 Evry Cedex, France
    BMC Biotechnol 9:35. 2009
    ..Gene targeting events were scored either following transient co-transfection of reporter plasmids and donor DNAs, or in a system where a reporter construct was stably integrated into the chromosome...
  6. ncbi request reprint Characterization of the gene transfer process mediated by histidine-rich peptides
    Antoine Kichler
    Genethon CNRS UMR 8115, 1bis rue de l Internationale, BP60, 91002 Evry, France
    J Mol Med (Berl) 85:191-201. 2007
    ..Taken together, the data also suggest that the peptides efficiently transport DNA into the cytoplasm and that the creation of more potent transfection compounds probably needs improvement of other steps during the process...
  7. ncbi request reprint Cationic amphipathic histidine-rich peptides for gene delivery
    Antoine Kichler
    Genethon UMR 8115, 1 rue de l Internationale, F 91002 Evry, France
    Biochim Biophys Acta 1758:301-7. 2006
    ....
  8. ncbi request reprint Cationic steroid antibiotics demonstrate DNA delivery properties
    Antoine Kichler
    Genethon UMR 8115 CNRS, 1bis rue de l Internationale, BP 60, F 91002 Evry, France
    J Control Release 107:174-82. 2005
    ..Collectively, our results show that DNA delivery agents possessing strong antibacterial properties can be obtained by conjugating amino groups to a steroid nucleus...
  9. ncbi request reprint Gene transfer with modified polyethylenimines
    Antoine Kichler
    Genethon CNRS UMR 8115, 1 rue de l Internationale, F 91002 Evry, France
    J Gene Med 6:S3-10. 2004
    ..Alternatively, incorporation of certain ligands into the DNA complexes also resulted in charge shielding even without PEGylation. Herein, I review the most recent PEI derivatives, with a special focus on PEGylated and targeted polymers...
  10. pmc Histidine-rich amphipathic peptide antibiotics promote efficient delivery of DNA into mammalian cells
    Antoine Kichler
    Généthon III Centre National de la Recherche Scientifique Unité de Recherche Associée 1923, 1 rue de l Internationale, F 91002 Evry, France
    Proc Natl Acad Sci U S A 100:1564-8. 2003
    ..Thus, this family of peptides represents a new class of agents that may have broad utility for gene transfer and gene therapy applications...
  11. ncbi request reprint Preparation and evaluation of a new class of gene transfer reagents: poly(-alkylaminosiloxanes)
    Antoine Kichler
    Genethon CNRS UMR 8115, 1 Rue de l Internationale F 91002 Evry, France
    J Control Release 93:403-14. 2003
    ..The transfection activity was sensitive to bafilomycin A1, suggesting a mechanism that depends on proton capture during the acidification process associated with endocytosis...
  12. ncbi request reprint Intranasal gene delivery with a polyethylenimine-PEG conjugate
    Antoine Kichler
    Genethon III CNRS URA 1923, 1 rue de l Internationale, Evry, France
    J Control Release 81:379-88. 2002
    ..Luciferase activity was greatest 24 h after delivery and decreased thereafter. Our results show that the grafting of PEGs can improve some of the properties of PEIs...
  13. ncbi request reprint Polyethylenimine-mediated gene delivery: a mechanistic study
    A Kichler
    Genethon III, CNRS URA 1923, Evry, France
    J Gene Med 3:135-44. 2001
    ..It has been hypothesized that the efficiency of PEI is due to its capacity to buffer the endosomes...
  14. doi request reprint Evaluation of the muscle gene transfer activity of a series of amphiphilic triblock copolymers
    Debborah Alimi-Guez
    FRE3087 CNRS Genethon, Evry, France
    J Gene Med 11:1114-24. 2009
    ..To better understand the way such copolymers act, we studied the behaviour of different poloxamers, including L64, both in vitro and in vivo...
  15. doi request reprint Soluble TNF-α receptor secretion from healthy or dystrophic mice after AAV6-mediated muscle gene transfer
    G Moulay
    Genethon, BP60, Evry, France
    Gene Ther 17:1400-10. 2010
    ..Together, our results underscore that the pathological state of the muscle has to be taken into consideration when designing gene therapy approaches...
  16. ncbi request reprint The cationic amphipathic alpha-helix of HIV-1 viral protein R (Vpr) binds to nucleic acids, permeabilizes membranes, and efficiently transfects cells
    Emmanuel Coeytaux
    Généthon Unité Mixte de Recherche 8115 CNRS, 1 bis rue de l Internationale, BP 60, F 91002 Evry, France
    J Biol Chem 278:18110-6. 2003
    ..Taken together, the results show that the cationic C-terminal alpha-helix of Vpr has DNA-condensing as well as membrane-destabilizing capabilities, both properties that are indispensable for efficient DNA transfection...
  17. doi request reprint Synthesis and evaluation of amphiphilic poly(tetrahydrofuran-b-ethylene oxide) copolymers for DNA delivery into skeletal muscle
    Catherine Pomel
    Laboratoire Matériaux Polymères aux Interfaces UMR CNRS 7581, Université d Evry, Evry, France
    Pharm Res 25:2963-71. 2008
    ..We were interested in studying the effect of a chemical change of the central block of pluronic polymers on the transfection activity...
  18. doi request reprint Real-time monitoring of cell transplantation in mouse dystrophic muscles by a secreted alkaline phosphatase reporter gene
    X Gerard
    FRE3087 CNRS Genethon, 1 rue de l Internationale, Evry, France
    Gene Ther 16:815-9. 2009
    ..Taken together, our results show that mSeAP is an excellent quantitative 'real-time' reporter gene for cell therapy preclinical studies...
  19. doi request reprint Design and evaluation of histidine-rich amphipathic peptides for siRNA delivery
    Bérangère Langlet-Bertin
    Genethon, 1bis rue de l Internationale, BP60, 91002 Evry, France
    Pharm Res 27:1426-36. 2010
    ..Since such peptides are thought to efficiently disrupt endosomal membranes, we have tested their ability to deliver small interfering RNA (siRNA) into mammalian cells...
  20. ncbi request reprint Self-promoted cellular uptake of peptide/DNA transfection complexes
    Lydia Prongidi-Fix
    Université Louis Pasteur CNRS, UMR 7177, Institut de Chimie de Strasbourg, 4, rue Blaise Pascal, 67070 Strasbourg, France
    Biochemistry 46:11253-62. 2007
    ..When these data are taken together, they indicate a dual role of the peptide during the transfection process, namely, DNA complexation and membrane permeabilization...
  21. ncbi request reprint [Antimicrobial peptides as efficient DNA vectors]
    Antoine Kichler
    Med Sci (Paris) 19:1046-7. 2003
  22. pmc Enhanced membrane disruption and antibiotic action against pathogenic bacteria by designed histidine-rich peptides at acidic pH
    A James Mason
    Faculte de Chimie, University Louis Pasteur, Institut Le Bel, Strasbourg, France
    Antimicrob Agents Chemother 50:3305-11. 2006
    ....
  23. ncbi request reprint Synthesis of linear polyethylenimine derivatives for DNA transfection
    Blandine Brissault
    Laboratory Matériaux Polymères aux Interfaces, UMR CNRS 7581, University of Evry, Bld Mitterrand, 91025 Evry Cedex, France
    Bioconjug Chem 14:581-7. 2003
    ....
  24. ncbi request reprint Membrane interaction of chrysophsin-1, a histidine-rich antimicrobial peptide from red sea bream
    A James Mason
    UMR 7177, Institut de Chimie, Université Louis Pasteur CNRS, 4 rue Blaise Pascal, 67070 Strasbourg, France
    Biochemistry 46:15175-87. 2007
    ..The combination of biophysical data and cell-based assays suggests likely mechanisms involved in both the antibiotic and toxic activity of chrysophsins...
  25. ncbi request reprint Amphiphilic poly[(propylene glycol)-block-(2-methyl-2-oxazoline)] copolymers for gene transfer in skeletal muscle
    Blandine Brissault
    Laboratoire Matériaux Polymères aux Interfaces UMR CNRS 7581, Université d Evry, 91025 Evry, France
    ChemMedChem 2:1202-7. 2007
    ..Overall, these results show that the capacity to enhance DNA transfection in skeletal muscle is not restricted to PEG-PPG-PEG arrangements...