Jean Michel Jault
Mathieu K, Javed W, Vallet S, Lesterlin C, Candusso M, Ding F, et al
. Functionality of membrane proteins overexpressed and purified from E. coli is highly dependent upon the strain. Sci Rep. 2019;9:2654 pubmed publisher
..Our results suggest that the amount of T7 RNA polymerase transcripts may indirectly but notably impact the structure and activity of overexpressed membrane proteins, and advise caution when using GFP fusion strategy. ..
Wicker Planquart C, Jault J. Interaction between Bacillus subtilis YsxC and ribosomes (or rRNAs). FEBS Lett. 2015;589:1026-32 pubmed publisher
..YsxC GTPase activity was stimulated upon 50S ribosomal subunit binding. In addition, it is shown for the first time that YsxC binds both 16S and 23S ribosomal RNAs. ..
Wicker Planquart C, Ceres N, Jault J. The C-terminal Î±-helix of YsxC is essential for its binding to 50S ribosome and rRNAs. FEBS Lett. 2015;589:2080-6 pubmed publisher
..Our results indicate that the basic C-terminal region of YsxC is required for its binding to the 50S ribosomal subunit. ..
Orelle C, Durmort C, Mathieu K, Duchêne B, Aros S, Fenaille F, et al
. A multidrug ABC transporter with a taste for GTP. Sci Rep. 2018;8:2309 pubmed publisher
..Importantly, the ATP and GTP concentrations are similar in S. pneumoniae supporting the physiological relevance of GTP as the energy source of this bacterial transporter. ..
Fribourg P, Chami M, Sorzano C, Gubellini F, Marabini R, Marco S, et al
. 3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment. J Mol Biol. 2014;426:2059-69 pubmed publisher
..Overall, the data are consistent with a mechanism of drug transport involving large conformational changes of BmrA and show that a bacterial ABC exporter can adopt at least two open inward conformations in lipid membrane...
Wiseman B, Jault J. Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA. Methods Mol Biol. 2016;1432:133-42 pubmed publisher
..We show that BmrA can be heterologously overexpressed in huge amount in E. coli and extracted from the membrane in a functionally active form. ..
Nguyen H, El Khoury T, Guiral S, Laaberki M, Candusso M, Galisson F, et al
. Expanding the Kinome World: A New Protein Kinase Family Widely Conserved in Bacteria. J Mol Biol. 2017;429:3056-3074 pubmed publisher
..These results expand the boundaries of the bacterial kinome and support the involvement of YdiB in protein translation and resistance to oxidative stress in B. subtilis. ..