William Vainchenker

Summary

Affiliation: Institut Gustave Roussy
Country: France

Publications

  1. ncbi request reprint RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes
    Magali Berthebaud
    Institut National de la Santé et de la Recherche Médicale INSERM U 362, Institut Gustave Roussy, Villejuif, France
    Blood 106:2962-8. 2005
  2. pmc Heterozygous and homozygous JAK2(V617F) states modeled by induced pluripotent stem cells from myeloproliferative neoplasm patients
    Joseph Saliba
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Recherche 1009, Laboratory of Excellence, Globule rouge Excellence GR Ex, Villejuif, France University Paris Sud 11, Le Kremlin Bicetre, France Institut Gustave Roussy, Villejuif, France
    PLoS ONE 8:e74257. 2013
  3. doi request reprint JAK/STAT signaling in hematological malignancies
    W Vainchenker
    INSERM, UMR 1009, Hématopoïèse Normale et Pathologique, Villejuif, France
    Oncogene 32:2601-13. 2013
  4. ncbi request reprint Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates
    N Debili
    Institut National de la Santé et de la Recherche Médicale INSERM U 362, Institut Gustave Roussy, Cedex, France
    Blood 97:2023-30. 2001
  5. doi request reprint New mutations and pathogenesis of myeloproliferative neoplasms
    William Vainchenker
    INSERM, UMR1009, Institut Gustave Roussy, Villejuif, France
    Blood 118:1723-35. 2011
  6. doi request reprint JAKs in pathology: role of Janus kinases in hematopoietic malignancies and immunodeficiencies
    William Vainchenker
    Institut Gustave Roussy PR 1, 94805 Villejuif, France
    Semin Cell Dev Biol 19:385-93. 2008
  7. pmc Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    William Raoul
    Unité INSERM TGU841 Université Paris XII, Creteil, France
    Respir Res 8:8. 2007
  8. doi request reprint MAL/SRF complex is involved in platelet formation and megakaryocyte migration by regulating MYL9 (MLC2) and MMP9
    Laure Gilles
    INSERM, U790, Villejuif, France
    Blood 114:4221-32. 2009
  9. doi request reprint A major role of TGF-beta1 in the homing capacities of murine hematopoietic stem cell/progenitors
    Claude Capron
    Inserm U1009, Faculté de Médecine Paris XI, Institut Gustave Roussy, Villejuif, France
    Blood 116:1244-53. 2010
  10. ncbi request reprint Monocyte/macrophage dysfunctions do not impair the promotion of myelofibrosis by high levels of thrombopoietin
    Orianne Wagner-Ballon
    Institut National de la Sante et de la Recherche Medicale INSERM U790, Pavillon de Recherche 1, Institut Gustave Roussy, Villejuif, France
    J Immunol 176:6425-33. 2006

Detail Information

Publications72

  1. ncbi request reprint RGS16 is a negative regulator of SDF-1-CXCR4 signaling in megakaryocytes
    Magali Berthebaud
    Institut National de la Santé et de la Recherche Médicale INSERM U 362, Institut Gustave Roussy, Villejuif, France
    Blood 106:2962-8. 2005
    ..Thus, our data reveal that RGS16 is a negative regulator of CXCR4 signaling in megakaryocytes. We postulate that RGS16 regulation is a mechanism that controls megakaryocyte maturation by regulating signals from the microenvironment...
  2. pmc Heterozygous and homozygous JAK2(V617F) states modeled by induced pluripotent stem cells from myeloproliferative neoplasm patients
    Joseph Saliba
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Recherche 1009, Laboratory of Excellence, Globule rouge Excellence GR Ex, Villejuif, France University Paris Sud 11, Le Kremlin Bicetre, France Institut Gustave Roussy, Villejuif, France
    PLoS ONE 8:e74257. 2013
    ..Altogether, this study brings the proof of concept that iPS can be used for studying MPN pathogenesis, clonal architecture, and drug efficacy. ..
  3. doi request reprint JAK/STAT signaling in hematological malignancies
    W Vainchenker
    INSERM, UMR 1009, Hématopoïèse Normale et Pathologique, Villejuif, France
    Oncogene 32:2601-13. 2013
    ....
  4. ncbi request reprint Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates
    N Debili
    Institut National de la Santé et de la Recherche Médicale INSERM U 362, Institut Gustave Roussy, Cedex, France
    Blood 97:2023-30. 2001
    ..Blood. 2001;97:2023-2030)..
  5. doi request reprint New mutations and pathogenesis of myeloproliferative neoplasms
    William Vainchenker
    INSERM, UMR1009, Institut Gustave Roussy, Villejuif, France
    Blood 118:1723-35. 2011
    ..Their precise roles in hematopoiesis and in the pathogenesis of MPN, as well as their prognostic impact and potential as a therapeutic target, are currently under investigation...
  6. doi request reprint JAKs in pathology: role of Janus kinases in hematopoietic malignancies and immunodeficiencies
    William Vainchenker
    Institut Gustave Roussy PR 1, 94805 Villejuif, France
    Semin Cell Dev Biol 19:385-93. 2008
    ..In contrast, loss-of-function mutations of JAK3 and TYK2 lead to immunodeficiency. The role of JAKs as therapeutic targets is starting to expand, as more insights into their structure and activation mechanisms become available...
  7. pmc Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    William Raoul
    Unité INSERM TGU841 Université Paris XII, Creteil, France
    Respir Res 8:8. 2007
    ..Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown...
  8. doi request reprint MAL/SRF complex is involved in platelet formation and megakaryocyte migration by regulating MYL9 (MLC2) and MMP9
    Laure Gilles
    INSERM, U790, Villejuif, France
    Blood 114:4221-32. 2009
    ..Finally, the use of a shRNA to decrease MYL9 expression showed that MYL9 was involved in proplatelet formation. MAL/SRF complex is thus involved in platelet formation and megakaryocyte migration by regulating MYL9 and MMP9...
  9. doi request reprint A major role of TGF-beta1 in the homing capacities of murine hematopoietic stem cell/progenitors
    Claude Capron
    Inserm U1009, Faculté de Médecine Paris XI, Institut Gustave Roussy, Villejuif, France
    Blood 116:1244-53. 2010
    ..Similar defects were found in liver cells from TGF-beta1(-/-) embryos on day 14 after vaginal plug. These data indicate that TGF-beta1 is a critical regulator for in vivo homeostasis of the HSCs, especially for their homing potential...
  10. ncbi request reprint Monocyte/macrophage dysfunctions do not impair the promotion of myelofibrosis by high levels of thrombopoietin
    Orianne Wagner-Ballon
    Institut National de la Sante et de la Recherche Medicale INSERM U790, Pavillon de Recherche 1, Institut Gustave Roussy, Villejuif, France
    J Immunol 176:6425-33. 2006
    ....
  11. pmc Ligand-independent thrombopoietin mutant receptor requires cell surface localization for endogenous activity
    Caroline Marty
    INSERM, U790, Villejuif 94805, France
    J Biol Chem 284:11781-91. 2009
    ....
  12. pmc An activating mutation in the CSF3R gene induces a hereditary chronic neutrophilia
    Isabelle Plo
    Research Laboratory on Hematopoiesis and Normal and Leukemic Stem Cells, U790, Institut National de la Sante et de la Recherche Medicale, 94805 Villejuif, France
    J Exp Med 206:1701-7. 2009
    ..Our data thus indicate that mutations in the CSF3R gene can be responsible for hereditary neutrophilia mimicking a myeloproliferative disorder...
  13. ncbi request reprint The SCL relative LYL-1 is required for fetal and adult hematopoietic stem cell function and B-cell differentiation
    Claude Capron
    Institut National de la Santé et de la Recherche Médicicale INSERM U362, Institut Gustave Roussy, 94805 Villejuif, France
    Blood 107:4678-86. 2006
    ..Our results demonstrate for the first time that Lyl-1 functions are important for HSC properties and B-cell differentiation and that they are largely distinct from scl functions...
  14. ncbi request reprint Differential signalling of NH2-terminal flag-labelled thrombopoietin receptor activated by TPO or anti-FLAG antibodies
    Gael A Millot
    INSERM U362, Institut Gustave Roussy, 39 rue Camille Desmoulins, Villejuif, France
    Cell Signal 16:355-63. 2004
    ..Since the anti-FLAG antibodies do not react with the TPO binding site of the receptor, we hypothesize that they can trigger a distinct signal by dimerizing Mpl in a manner different from that induced by TPO...
  15. doi request reprint Myeloproliferative neoplasm induced by constitutive expression of JAK2V617F in knock-in mice
    Caroline Marty
    Inserm U1009, Institut Gustave Roussy, PR1, 114 rue Edouard Vaillant, 94805 Villejuif, France
    Blood 116:783-7. 2010
    ..In conclusion, constitutive heterozygous expression of JAK2(V617F) in mice is not embryo-lethal but results in severe PV-like disease with secondary myelofibrosis and not in ET-like disease as expected from patient study...
  16. ncbi request reprint Distinct effects of the soluble versus membrane-bound forms of the notch ligand delta-4 on human CD34+CD38low cell expansion and differentiation
    Medhi Lahmar
    U790 INSERM, Institut Gustave Roussy, University Paris XI, PR1, 94800 Villejuif, France
    Stem Cells 26:621-9. 2008
    ..Altogether, these results suggest that part of solD4 activity did not rely upon canonical Notch pathway...
  17. ncbi request reprint Identification of CXCR4 as a new nitric oxide-regulated gene in human CD34+ cells
    Yanyan Zhang
    INSERM U790, Institut Gustave Roussy, PR1, Villejuif, France
    Stem Cells 25:211-9. 2007
    ..This study demonstrates that the NO pathway can modulate CXCR4 expression in human CD34(+) cells and suggests that NO may play a critical role in the trafficking of hematopoietic progenitors...
  18. ncbi request reprint A unique clonal JAK2 mutation leading to constitutive signalling causes polycythaemia vera
    Chloe James
    INSERM U362, Institut Gustave Roussy, Paris XI University, PR1, 39 rue Camille Desmoulins, 94805 Villejuif Cedex, France
    Nature 434:1144-8. 2005
    ..As this mutation is also found in other myeloproliferative disorders, this unique mutation will permit a new molecular classification of these disorders and novel therapeutical approaches...
  19. ncbi request reprint JAK2V617F expression in murine hematopoietic cells leads to MPD mimicking human PV with secondary myelofibrosis
    Catherine Lacout
    Institut National de la Sante et de la Recherche Medicale INSERM U790, Universite Paris XI, Institut Gustave Roussy IGR, Villejuif, France
    Blood 108:1652-60. 2006
    ..Questions remain regarding the exact contribution of JAK2(V617F) in other myeloproliferative disorders...
  20. ncbi request reprint Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach
    Hana Raslova
    INSERM Unité 790, Institut Gustave Roussy, 1 rue Camille Desmoulins, 94805 Villejuif, France
    Blood 109:3225-34. 2007
    ..Together, these results suggest that MK polyploidization per se does not regulate gene expression but is intrinsically included in the differentiation process...
  21. ncbi request reprint Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4-/- chimeric mice
    Adlen Foudi
    INSERM U362, IFR54, Institut Gustave Roussy, Villejuif Cedex, France
    Blood 107:2243-51. 2006
    ..Together, the data strongly suggest that impaired retention may, at least in short-term hematopoietic reconstitution, lead to a diminution in the number of available progenitors required for radioprotection...
  22. ncbi request reprint p210BCR-ABL inhibits SDF-1 chemotactic response via alteration of CXCR4 signaling and down-regulation of CXCR4 expression
    Jean Francois Geay
    Institut National de la Sante et de la Recherche Medicale, Institut Gustave Roussy, Villejuif, France
    Cancer Res 65:2676-83. 2005
    ..Collectively, these data indicate that p210(BCR-ABL) could affect CXCR4 by more than one mechanism and suggest that down-regulation of CXCR4 may have important implications in chronic myelogenous leukemia pathogenesis...
  23. ncbi request reprint Deficiency in the Wiskott-Aldrich protein induces premature proplatelet formation and platelet production in the bone marrow compartment
    Siham Sabri
    Institut National de la Santé etde la Recherche Médicale INSERM, U790, Universite Paris XI, Institut Gustave Roussy, Villejut, France
    Blood 108:134-40. 2006
    ..In addition, they suggest a novel explanation for some of the platelet abnormalities characteristic of patients with WAS...
  24. ncbi request reprint Proplatelet formation is regulated by the Rho/ROCK pathway
    Yunhua Chang
    Institut National de la Sante et de la Recherche Medicale, Unité U 790, Pavillon de Recherche 1, Institut Gustave Roussy, Villejuif, France
    Blood 109:4229-36. 2007
    ..Taken together, our results suggest that MLC2 phosphorylation is regulated by both ROCK and MLC kinase and plays an important role in platelet biogenesis by controlling PPF and fragmentation...
  25. ncbi request reprint Gfi-1B plays a critical role in terminal differentiation of normal and transformed erythroid progenitor cells
    Loic Garcon
    Institut National de la Santé et de la Recherche Médicale INSERM U362, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Blood 105:1448-55. 2005
    ..Deletion of the SNAG repressor domain abolished Gfi-1B-induced erythroid maturation, strongly suggesting that Gfi-1B acts in the late stage of erythroid differentiation as a transcriptional repressor...
  26. doi request reprint Megakaryocyte endomitosis is a failure of late cytokinesis related to defects in the contractile ring and Rho/Rock signaling
    Larissa Lordier
    INSERM, U790, Villejuif, France
    Blood 112:3164-74. 2008
    ..Our results suggest that the late failure of cytokinesis responsible for the endomitotic process is related to a partial defect in the Rho/Rock pathway activation...
  27. doi request reprint Selective reduction of JAK2V617F-dependent cell growth by siRNA/shRNA and its reversal by cytokines
    Abire Jedidi
    INSERM, Unité 790, Institut Gustave Roussy, Villejuif, France
    Blood 114:1842-51. 2009
    ..This strongly suggests that patient cytokine levels in current JAK2 inhibitor clinical trials modulate the outcome of these therapies...
  28. pmc FLI1 monoallelic expression combined with its hemizygous loss underlies Paris-Trousseau/Jacobsen thrombopenia
    Hana Raslova
    Institut National de la Santé et de la Recherche Médicale U 362, Villejuif, France
    J Clin Invest 114:77-84. 2004
    ....
  29. ncbi request reprint Spontaneous STAT5 activation induces growth factor independence in idiopathic myelofibrosis: possible relationship with FKBP51 overexpression
    Emiko Komura
    INSERM U362, Pavillon de Recherche 1, Institut Gustave Roussy, Villejuif Cedex, France
    Exp Hematol 31:622-30. 2003
    ..They also suggest that FKBP51 overexpression could be involved in STAT5 activation in IMF cells and in subsequent abnormal growth...
  30. ncbi request reprint Adenoviral-mediated TGF-beta1 inhibition in a mouse model of myelofibrosis inhibit bone marrow fibrosis development
    Thomas Gastinne
    INSERM U790, Pavillon de Recherche 1, Institut Gustave Roussy, Villejuif Cedex, France
    Exp Hematol 35:64-74. 2007
    ..However, lethality of TPO overexpression was not decreased after treatment, indicating that in this mice model, myeloproliferation rather than fibrosis was probably responsible for the lethality induced by the disorder...
  31. ncbi request reprint Stimulation of osteoprotegerin production is responsible for osteosclerosis in mice overexpressing TPO
    Hedia Chagraoui
    IFR 54 INSERM U 362, Institut Gustave Roussy, Villejuif, France
    Blood 101:2983-9. 2003
    ..These findings suggest that osteosclerosis in mice with TPO overexpression occurs predominantly via an up-regulation of OPG in host stromal cells leading to disruption of osteoclastogenesis...
  32. ncbi request reprint Evidence for MPL W515L/K mutations in hematopoietic stem cells in primitive myelofibrosis
    Ronan Chaligne
    Institut National de la Sante et de la Recherche Medicale INSERM, U790, Universite Paris XI, Institut Gustave Roussy, Villejuif, France
    Blood 110:3735-43. 2007
    ..In contrast, erythroid progenitors remained EPO dependent. These results demonstrate that in PMF, the MPL W515L or K mutation induces a spontaneous megakaryocyte (MK) differentiation and occurs in a multipotent HSCs...
  33. ncbi request reprint Intracellular localization and constitutive endocytosis of CXCR4 in human CD34+ hematopoietic progenitor cells
    Yanyan Zhang
    INSERM U362, Institut Gustave Roussy, PR1, 39 rue Camille Desmoulins, 94805 Villejuif, France
    Stem Cells 22:1015-29. 2004
    ....
  34. ncbi request reprint Expression of osteoprotegerin mRNA and protein in murine megakaryocytes
    Hedia Chagraoui
    INSERM U362, IFR 54, Institut Gustave Roussy, Villejuif, France
    Exp Hematol 31:1081-8. 2003
    ..However, OPG expression and regulation in primary hematopoietic cells have not been fully investigated...
  35. ncbi request reprint Dysmegakaryopoiesis of FPD/AML pedigrees with constitutional RUNX1 mutations is linked to myosin II deregulated expression
    Dominique Bluteau
    Institut National de la Sante et de la Recherche Medicale, Villejuif, France
    Blood 120:2708-18. 2012
    ..Together, these results demonstrate that the defects in megakaryopoiesis observed in FPD/AML are, in part, related to a deregulation of myosin IIA and IIB expression leading to both a defect in ploidization and proplatelet formation...
  36. doi request reprint LYL-1 deficiency induces a stress erythropoiesis
    Claude Capron
    Inserm U1009, IFR 54, Institut Gustave Roussy, Villejuif, France
    Exp Hematol 39:629-42. 2011
    ..Because LYL-1 is expressed in erythroid cell populations, we addressed its role in erythropoiesis using knockin mice...
  37. doi request reprint Monocytic cells derived from human embryonic stem cells and fetal liver share common differentiation pathways and homeostatic functions
    Olena Klimchenko
    INSERM, Institut Gustave Roussy, Villejuif, France
    Blood 117:3065-75. 2011
    ..Trophic and immunosuppressive functions of M2-polarized macrophages link fetus and tumor development, and hESCs offer a valuable experimental model for in vitro studies of mechanisms sustaining these processes...
  38. ncbi request reprint Evaluation of hematopoietic potential generated by transplantation of muscle-derived stem cells in mice
    Francoise Farace
    Translational Research Cell Therapy Laboratory, Department of Clinical Biology, Institut Gustave Roussy, Villejuif, France
    Stem Cells Dev 13:83-92. 2004
    ....
  39. ncbi request reprint Proteasome inhibitor bortezomib impairs both myelofibrosis and osteosclerosis induced by high thrombopoietin levels in mice
    Orianne Wagner-Ballon
    Institut National de la Sante et de la Recherche Medicale, U790, Universite Paris XI, Villejuif, France
    Blood 110:345-53. 2007
    ..Finally, bortezomib dramatically improved TPO(high) mouse survival (89% vs 8% at week 52). We conclude that bortezomib appears as a promising therapy for future treatment of PMF patients...
  40. doi request reprint The hematopoietic stem cell compartment of JAK2V617F-positive myeloproliferative disorders is a reflection of disease heterogeneity
    Chloe James
    INSERM, U876, Bordeaux, France
    Blood 112:2429-38. 2008
    ..These experiments provide new insights into the pathogenesis of JAK2V617F MPD and demonstrate that a JAK2 inhibitor needs to target the HSC compartment for optimal disease control in classical MPD...
  41. ncbi request reprint Quantitative and qualitative in vitro analysis of the stem cell potential of hematopoietic cells purified from murine skeletal muscle
    Celine Haond
    Inserm U362 and Translational Research Laboratory, Institut Gustave Roussy, Villejuif, France
    Cell Res 17:783-91. 2007
    ....
  42. ncbi request reprint The JAK2 617V>F mutation triggers erythropoietin hypersensitivity and terminal erythroid amplification in primary cells from patients with polycythemia vera
    Sabrina Dupont
    INSERM Unité 790, Universite Paris Sud, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif, France
    Blood 110:1013-21. 2007
    ....
  43. ncbi request reprint Role for the nuclear factor kappaB pathway in transforming growth factor-beta1 production in idiopathic myelofibrosis: possible relationship with FK506 binding protein 51 overexpression
    Emiko Komura
    Institut National de la Sante et de la Recherche Medicale U362, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805, Villejuif Cedex, France
    Cancer Res 65:3281-9. 2005
    ..They also suggest that FKBP51's overexpression in IMF cells could play an important role in the pathogenesis of this myeloproliferative disorder...
  44. doi request reprint P19INK4D links endomitotic arrest and megakaryocyte maturation and is regulated by AML-1
    Laure Gilles
    Institut National de la Santé et de la Recherche Médicale U790, Villejuif, France
    Blood 111:4081-91. 2008
    ..Moreover, AML-1 inhibition led to the p19(INK4D) down-regulation in human MKs. These results may explain the molecular link at the transcriptional level between the arrest of endomitosis and the acceleration of MK differentiation...
  45. ncbi request reprint BCR-ABL activates STAT3 via JAK and MEK pathways in human cells
    Paul Coppo
    INSERM U362 Unité Hématopoïèse et Cellules Souches, Institut Gustave Roussy, Villejuif, France
    Br J Haematol 134:171-9. 2006
    ..Targeting JAK, MEK and STAT3 pathways could therefore be of therapeutic value, especially in advanced stage CML...
  46. doi request reprint JAK2V617F expression in mice amplifies early hematopoietic cells and gives them a competitive advantage that is hampered by IFNα
    Salma Hasan
    INSERM U 1009, Institut Gustave Roussy, Villejuif, France
    Blood 122:1464-77. 2013
    ....
  47. pmc Inhibition of TET2-mediated conversion of 5-methylcytosine to 5-hydroxymethylcytosine disturbs erythroid and granulomonocytic differentiation of human hematopoietic progenitors
    Elodie Pronier
    INSERM, U1009, Institut Gustave Roussy, Villejuif, France
    Blood 118:2551-5. 2011
    ..Our results indicate that TET2 disruption affects 5-hmC levels in human myeloid cells and participates in the pathogenesis of myeloid malignancies through the disturbance of myeloid differentiation...
  48. doi request reprint BCR-ABL induces opposite phenotypes in murine ES cells according to STAT3 activation levels
    Paul Coppo
    INSERM, Unités Mixtes de Recherche UMR 790, Institut Gustave Roussy, Universite Paris XI, Villejuif, France
    Cell Signal 21:52-60. 2009
    ....
  49. ncbi request reprint MYH10 protein expression in platelets as a biomarker of RUNX1 and FLI1 alterations
    Iléana Antony-Debré
    Institut National de la Sante et de la Recherche Medicale, Unité Mixte de Recherche UMR 1009, Villejuif, France
    Blood 120:2719-22. 2012
    ..We propose MYH10 detection as a new and simple tool to identify inherited platelet disorders and myeloid neoplasms with abnormalities in RUNX1 and its associated proteins...
  50. doi request reprint Down-regulation of the RUNX1-target gene NR4A3 contributes to hematopoiesis deregulation in familial platelet disorder/acute myelogenous leukemia
    Dominique Bluteau
    INSERM, U1009, Villejuif, France
    Blood 118:6310-20. 2011
    ..We propose that the down-regulation of NR4A3 in RUNX1-mutated hematopoietic progenitors leads to an increase in the pool of cells susceptible to be hit by secondary leukemic genetic events...
  51. ncbi request reprint Constitutive and specific activation of STAT3 by BCR-ABL in embryonic stem cells
    Paul Coppo
    INSERM U362, Translational Research Cell Therapy Laboratory, Villejuif, Cedex, France
    Oncogene 22:4102-10. 2003
    ..Thus, the BCR-ABL embryonic stem cell model that we developed as well as the results obtained in human CML samples suggests a role for STAT3 in the pathogenesis of human CML...
  52. doi request reprint Aurora B is dispensable for megakaryocyte polyploidization, but contributes to the endomitotic process
    Larissa Lordier
    Inserm U1009, Institut Gustave Roussy, 39 rue Camille Desmoulins, Villejuif, France
    Blood 116:2345-55. 2010
    ..This indicates that Aurora B is dispensable for MK polyploidization but is necessary to achieve a normal endomitotic process...
  53. doi request reprint A common bipotent progenitor generates the erythroid and megakaryocyte lineages in embryonic stem cell-derived primitive hematopoiesis
    Olena Klimchenko
    INSERM U790, Institut Gustave Roussy, Villejuif, France
    Blood 114:1506-17. 2009
    ..This novel MEP differentiation system may allow an in-depth exploration of the molecular bases of erythroid and MK commitment and differentiation...
  54. doi request reprint Mutation in TET2 in myeloid cancers
    Francois Delhommeau
    INSERM U790, Institut Gustave Roussy, Villejuif, France
    N Engl J Med 360:2289-301. 2009
    ..The myelodysplastic syndromes and myeloproliferative disorders are associated with deregulated production of myeloid cells. The mechanisms underlying these disorders are not well defined...
  55. ncbi request reprint Constitutive activation of STAT5 and Bcl-xL overexpression can induce endogenous erythroid colony formation in human primary cells
    Loic Garcon
    Unité U 790 Institut National de la Santé et de la Recherche Médicale INSERM, Institut Gustave Roussy, Villejuif, France
    Blood 108:1551-4. 2006
    ....
  56. doi request reprint Prognostic score including gene mutations in chronic myelomonocytic leukemia
    Raphael Itzykson
    Institut Gustave Roussy, Villejuif, France
    J Clin Oncol 31:2428-36. 2013
    ..We developed and validated a prognostic score for overall survival (OS) based on mutational status and standard clinical variables...
  57. pmc Apoptosis regulation in tetraploid cancer cells
    Maria Castedo
    CNRS, UMR8125, Institut Gustave Roussy, Villejuif, France
    EMBO J 25:2584-95. 2006
    ..Tetraploid cells were also more resistant against UVC and gamma-irradiation. These data indicate the existence of p53-dependent alterations in apoptosis regulation in tetraploid cells...
  58. doi request reprint Notch/Delta4 signaling inhibits human megakaryocytic terminal differentiation
    Sonia Poirault-Chassac
    Inserm U1009, Villejuif, France
    Blood 116:5670-8. 2010
    ..This blockade was accompanied by a modulation of the transcriptional program of megakaryocytic differentiation. All these results indicate that Dll4/Notch signaling inhibits human terminal MK differentiation...
  59. ncbi request reprint A partial down-regulation of WASP is sufficient to inhibit podosome formation in dendritic cells
    Aurelie Olivier
    Unité mixte INSERM Université Paris XI, U362, Institut Gustave Roussy, Villejuif F 94805 and CNRS, UMR 8115, Genethon, 91002 Evry, France
    Mol Ther 13:729-37. 2006
    ....
  60. ncbi request reprint Mammalian target of rapamycin (mTOR) regulates both proliferation of megakaryocyte progenitors and late stages of megakaryocyte differentiation
    Hana Raslova
    INSERM U790, Institut Gustave Roussy PR1, Villejuif, France
    Blood 107:2303-10. 2006
    ..These findings indicate that the mTOR pathway plays an important role during megakaryopoiesis by regulating ploidy, cell size, and maturation, in part by regulating p21 and cyclin D3...
  61. pmc A senescence-like cell-cycle arrest occurs during megakaryocytic maturation: implications for physiological and pathological megakaryocytic proliferation
    Rodolphe Besancenot
    INSERM, U790, Institut Gustave Roussy, Villejuif, France
    PLoS Biol 8:. 2010
    ..An escape from this physiological process may be associated with certain myeloproliferative neoplasms leading to abnormal megakaryocytic proliferation...
  62. ncbi request reprint Platelet formation is the consequence of caspase activation within megakaryocytes
    Stephane De Botton
    Institut National de la Santé et de la Recherche Médicale U362 and the Centre National de la Recherche Scientifique Unité Mixte de Recherche 1599, Institut Gustave Roussy, Institut Fédératif de Recherche 54, Villejuif, France
    Blood 100:1310-7. 2002
    ..Thus, localized caspase activation is causal to proplatelet formation. We conclude that proplatelet formation is regulated by a caspase activation limited to only some cellular compartments...
  63. ncbi request reprint Prominent role of TGF-beta 1 in thrombopoietin-induced myelofibrosis in mice
    Hedia Chagraoui
    INSERM U362, Institut Gustave Roussy, Villejuif, France
    Blood 100:3495-503. 2002
    ..These data provide evidence that TGF-beta1 produced by hematopoietic cells is pivotal for the pathogenesis of myelofibrosis that develops in mice with TPO overexpression...
  64. ncbi request reprint Quantitative Oct4 overproduction in mouse embryonic stem cells results in prolonged mesoderm commitment during hematopoietic differentiation in vitro
    Valerie Camara-Clayette
    Institut Gustave Roussy, INSERM U790, PR1, 94805 Villejuif Cedex, France
    Stem Cells 24:1937-45. 2006
    ..These results demonstrate that Oct4 acts as a master regulator of ESC differentiation...
  65. ncbi request reprint Microarray analysis of LIF/Stat3 transcriptional targets in embryonic stem cells
    Dalila Sekkai
    INSERM U362, Institut Gustave Roussy, 94805 Villejuif Cedex, France
    Stem Cells 23:1634-42. 2005
    ..Furthermore, chromatin immunoprecipitation experiment demonstrated that Stat3 is recruited to the promoter of aes1 in ES cells. These data demonstrated that the aes1 gene is a direct transcriptional target of Stat3 in ES cells...
  66. ncbi request reprint Isolation and culture of megakaryocyte precursors
    Najet Debili
    Institut Gustave Roussy, Institut Fédéeratif de Recherche, Villejuif, France
    Methods Mol Biol 272:293-308. 2004
  67. doi request reprint RUNX1-induced silencing of non-muscle myosin heavy chain IIB contributes to megakaryocyte polyploidization
    Larissa Lordier
    INSERM UMR 1009, Institut Gustave Roussy, 114, rue Edouard Vaillant, Villejuif 94805, France
    Nat Commun 3:717. 2012
    ..Thus, RUNX1-mediated silencing of MYH10 is required for the switch from mitosis to endomitosis, linking polyploidization with megakaryocyte differentiation...
  68. doi request reprint JAK2 stimulates homologous recombination and genetic instability: potential implication in the heterogeneity of myeloproliferative disorders
    Isabelle Plo
    INSERM, Unités Mixtes de Recherche 790, Villejuif, France
    Blood 112:1402-12. 2008
    ..Our study might provide some keys to understand how a single mutation can give rise to different pathologies...
  69. doi request reprint TET2 inactivation results in pleiotropic hematopoietic abnormalities in mouse and is a recurrent event during human lymphomagenesis
    Cyril Quivoron
    INSERM, U, Villejuif, France
    Cancer Cell 20:25-38. 2011
    ....
  70. doi request reprint The transcription factor Srf regulates hematopoietic stem cell adhesion
    Christine Ragu
    INSERM, U985, IGR, Villejuif, France
    Blood 116:4464-73. 2010
    ..Altogether, our results demonstrate that Srf is an essential regulator of stem cells and MPP adhesion, and suggest that Srf acts mainly through cell-matrix interactions and integrin signaling...
  71. ncbi request reprint Differential regulation of actin stress fiber assembly and proplatelet formation by alpha2beta1 integrin and GPVI in human megakaryocytes
    Siham Sabri
    Institut National de la Sante et de la Recherche Medicale, U362, Institut Gustave Roussy, rue Camille Desmoulins, 94800 Villejuif, France
    Blood 104:3117-25. 2004
    ..Thus, proplatelet formation in human MKs could be tightly regulated by differential interactions with their collagen receptors. We propose that this interaction with collagen prevents proplatelet formation within the marrow...
  72. ncbi request reprint Mitotic catastrophe constitutes a special case of apoptosis whose suppression entails aneuploidy
    Maria Castedo
    CNRS UMR 8125, Institut Gustave Roussy, 39 rue Camille Desmoulins, F 94805 Villejuif, France
    Oncogene 23:4362-70. 2004
    ..Our findings delineate a molecular pathway through which DNA damage, failure to arrest the cell cycle and inhibition of apoptosis can favor the occurrence of cytogenetic abnormalities that are likely to participate in oncogenesis...