Genomes and Genes
Jean Paul Thiery
Affiliation: Institut Curie
- Clinical and biological characteristics of cervical neoplasias with FGFR3 mutationChristophe Rosty
Departement de Pathologie, Institut Curie, Section Médicale, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Mol Cancer 4:15. 2005..To further analyze the role of FGFR3 in cervical tumor progression, we extended our study to screen a total of 75 invasive tumors and 80 cervical intraepithelial neoplasias (40 low-grade and 40 high-grade lesions)...
- Enrichment methods to detect bone marrow micrometastases in breast carcinoma patients: clinical relevanceValerie Choesmel
UMR144 CNRS, Research Division, Institut Curie, Paris, France
Breast Cancer Res 6:R556-70. 2004..In the present study, we have assessed the clinical relevance of current methods aimed at detecting rare disseminated carcinoma cells...
- Complex networks orchestrate epithelial-mesenchymal transitionsJean Paul Thiery
Centre National de la Recherche Scientifique CNRS Unité Mixte Recherche UMR 144 and Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Nat Rev Mol Cell Biol 7:131-42. 2006..Understanding how mesenchymal cells arise from an epithelial default status will also have a strong impact in unravelling the mechanisms that control fibrosis and cancer progression...
- Targeted activation of beta-catenin signaling in basal mammary epithelial cells affects mammary development and leads to hyperplasiaJérôme Teulière
UMR 144 CNRS Institut Curie, Institut Curie, Section de Recherche, 26 Rue d Ulm, 75248, Paris, Cedex 05, France
Development 132:267-77. 2005....
- Targets of fibroblast growth factor 1 (FGF-1) and FGF-2 signaling involved in the invasive and tumorigenic behavior of carcinoma cellsClotilde Billottet
Laboratory of Cell Morphogenesis and Tumor Progression, UMR 144 CNRS, Institut Curie, Section de Recherche, 75248 Paris Cedex 05, France
Mol Biol Cell 15:4725-34. 2004..These molecular analyses show that the potential of these two growth factors in tumor progression is highly dependent on specific FGFR signaling and highlights its importance as a target for antitumor therapy...
- Conditional beta1-integrin gene deletion in neural crest cells causes severe developmental alterations of the peripheral nervous systemThomas Pietri
UMR144, CNRS, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Development 131:3871-83. 2004..Thus, beta1-integrins are important for the control of embryonic and postnatal peripheral nervous system development...
- Beta1 integrin deletion from the basal compartment of the mammary epithelium affects stem cellsIlaria Taddei
Institut Curie, Centre de Recherche, Centre National de la Recherche Scientifique UMR144, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Nat Cell Biol 10:716-22. 2008....
- Isolation of mouse mammary epithelial progenitor cells with basal characteristics from the Comma-Dbeta cell lineMarie Ange Deugnier
Institut Curie, UMR144 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Dev Biol 293:414-25. 2006..These data provide evidence for the existence of basal-type mouse mammary progenitors able to participate in the morphogenetic processes characteristic of mammary gland development...
- Nuclear FGF-2 facilitates cell survival in vitro and during establishment of metastasesRachel J Thomas-Mudge
UMR144 CNRS, Institut Curie, Research Division, 26 Rue d Ulm, 75248 Paris 05, France
Oncogene 23:4771-9. 2004..This study provides both in vitro and in vivo evidence that the role of the nuclear 24 kDa FGF-2 isoform in carcinoma is the promotion of cell survival, thereby defining its association with poor prognosis in some human carcinomas...
- Integrins stimulate E-cadherin-mediated intercellular adhesion by regulating Src-kinase activation and actomyosin contractilityClara Martinez-Rico
UMR144 CNRS Institut Curie, 75248 Paris Cedex 05, France
J Cell Sci 123:712-22. 2010..These findings highlight the importance and mechanisms of molecular crosstalk between cadherins and integrins in the control of cell plasticity during histogenesis and morphogenesis...
- A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchymeMarie Anne Breau
Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Proc Natl Acad Sci U S A 105:7750-5. 2008....
- Modulation of several waves of gene expression during FGF-1 induced epithelial-mesenchymal transition of carcinoma cellsClotilde Billottet
CNRS UMR 144 Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Cell Biochem 104:826-39. 2008....
- Fgf10 expression identifies parabronchial smooth muscle cell progenitors and is required for their entry into the smooth muscle cell lineageArnaud A Mailleux
UMR144 CNRS Institut Curie, 75248 Paris Cedex 05, France
Development 132:2157-66. 2005..We provide support for a role of epithelial BMP4 in mediating the formation of parabronchial smooth muscle cells...
- Integrins in mammary gland development and differentiation of mammary epitheliumIlaria Taddei
UMR 144 CNRS-Institut Curie, Section de Recherche, 26, rue d'Ulm, 75248 Paris Cedex 05, France
J Mammary Gland Biol Neoplasia 8:383-94. 2003....
- Inhibition of human bladder tumour cell growth by fibroblast growth factor receptor 2b is independent of its kinase activity. Involvement of the carboxy-terminal region of the receptorIsabelle Bernard-Pierrot
UMR144, CNRS Institut Curie, Section de Recherche, 26 Rue d Ulm, 75248 Paris Cedex, France
Oncogene 23:9201-11. 2004..Our data suggest that FGFR2b inhibits the growth of bladder carcinoma cells by reducing IGF-II levels via its carboxy-terminal domain, independent of its tyrosine kinase activity...
- Fast dissociation kinetics between individual E-cadherin fragments revealed by flow chamber analysisEmilie Perret
Laboratoire de Morphogenèse Cellulaire et Différenciation Tumorale, UMR 144, CNRS Institut Curie, 26 Rue d Ulm, F 75248 Paris Cedex 05, France
EMBO J 21:2537-46. 2002..A fast off rate and the low tensile strength of the E-cadherin bond may be necessary to support the high selectivity and plasticity of epithelial cell interactions...
- Real-time quantitative PCR determination of urokinase-type plasminogen activator receptor (uPAR) expression of isolated micrometastatic cells from bone marrow of breast cancer patientsJean Yves Pierga
Medical Oncology Department, Institut Curie, 26 Rue d Ulm, 75231 Paris Cedex 05, France
Int J Cancer 114:291-8. 2005..A larger study would be useful to confirm the importance of uPAR to define higher risk subgroups of breast cancer patients with micrometastatic disease...
- Regional copy number-independent deregulation of transcription in cancerNicolas Stransky
UMR 144 Centre National de la Recherche Scientifique (CNRS)/Institut Curie, 75248 Paris Cedex 05, France
Nat Genet 38:1386-96. 2006..We validated one candidate region experimentally, demonstrating histone methylation leading to the loss of expression of neighboring genes without DNA methylation...
- Frequent PTEN genomic alterations and activated phosphatidylinositol 3-kinase pathway in basal-like breast cancer cellsBérengère Marty
Département de Transfert, Institut Curie, 26 Rue d Ulm, 75005 Paris, France
Breast Cancer Res 10:R101. 2008..In contrast to HER2+ carcinomas, no targeted therapy is currently available for the treatment of patients with BLCs. In order to discover potential therapeutic targets, we aimed to discover deregulated signalling pathways in human BLCs...
- Johnson-Kendall-Roberts theory applied to living cellsYeh-Shiu Chu
UMR 144, Centre National de la Recherche Scientifique et Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
Phys Rev Lett 94:028102. 2005..When the cytoskeleton is disrupted, JKR theory is no longer applicable...
- Cell adhesion in development: a complex signaling networkJean Paul Thiery
Unit Mixte de Recherche, 144 Centre National Recherche Scientifique, Institut Curie, 26 Rue d Ulm, 75248 Cedex 05, France
Curr Opin Genet Dev 13:365-71. 2003..Clearly, a wide variety of new biophysical techniques and genomic approaches will permit analysis of the roles of adhesive interactions in development to be addressed with far greater precision...
- Oncogenic properties of the mutated forms of fibroblast growth factor receptor 3bIsabelle Bernard-Pierrot
UMR 144, CNRS-Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, and INSERM 0337 and Service d'Urologie, Centre Hospitalier Universitaire Henri Mondor, AP-HP, , , France
Carcinogenesis 27:740-7. 2006..We showed using siRNA and SU5402, an FGFR inhibitor, that the tumour properties of MGH-U3 depended on mutated receptor activity. Thus, in two different models, mutated FGFR3b presents oncogenic properties...
- YY1 regulates the neural crest-associated slug gene in Xenopus laevisMatthew J Morgan
Institut Curie CNRS UMR144, Morphogenèse cellulaire et progression tumorale, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Biol Chem 279:46826-34. 2004....
- Epithelial-mesenchymal transitions in development and pathologiesJean Paul Thiery
Unite Mixte de Recherche 144, Centre National Recherche Scientifique, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Curr Opin Cell Biol 15:740-6. 2003..Current studies further emphasise the critical importance of EMT and provide a better molecular and functional definition of mesenchymal cells and how they emerged >500 million years ago as a key event in evolution...
- High-resolution mapping of DNA breakpoints to define true recurrences among ipsilateral breast cancersMarc A Bollet
Département d Oncologie Radiothérapie, Institut Curie, 26, rue d Ulm, 75248 Paris Cedex 05, France
J Natl Cancer Inst 100:48-58. 2008..To distinguish new primary breast cancers from true recurrences, pangenomic analyses of DNA copy number alterations (CNAs) using single-nucleotide polymorphism arrays have proven useful...
- Separation force measurements reveal different types of modulation of E-cadherin-based adhesion by nectin-1 and -3Clara Martinez-Rico
UMR 144 CNRS Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Biol Chem 280:4753-60. 2005....
- EGF controls the in vivo developmental potential of a mammary epithelial cell line possessing progenitor propertiesMarie-Ange Deugnier
CNRS-Institut Curie, Section de Recherche, 26 rue d'Ulm, 75248 Paris Cedex 05, France
J Cell Biol 159:453-63. 2002....
- beta-Catenin regulates P-cadherin expression in mammary basal epithelial cellsMarisa M Faraldo
UMR 144, CNRS Institut Curie, Section de Recherche, 26, rue d Ulm, 75248 Paris, Cedex 05, France
FEBS Lett 581:831-6. 2007..These data suggest that beta-catenin-dependent modulation of P-cadherin expression can contribute to the establishment of the basal phenotype...
- Regulation of XSnail2 expression by Rho GTPasesFlorence Broders-Bondon
Laboratoire de Morphogenèse Cellulaire et Progression Tumorale, UMR144 CNRS Institut Curie, Paris, France
Dev Dyn 236:2555-66. 2007..These results show that Rho GTPases can regulate the expression of XSnail2 during NC ontogeny...
- Analysis of array CGH data: from signal ratio to gain and loss of DNA regionsPhilippe Hupe
Service Bioinformatique, UMR 144 CNRS Institut Curie, 26, rue d Ulm, Paris, 75248 Cedex 05, France
Bioinformatics 20:3413-22. 2004..The achievement of these goals requires a methodology for detecting the breakpoints delimiting altered regions in genomic patterns and assigning a status (normal, gained or lost) to each chromosomal region...
- Identification of a proliferation gene cluster associated with HPV E6/E7 expression level and viral DNA load in invasive cervical carcinomaChristophe Rosty
, Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
Oncogene 24:7094-104. 2005..HPV expression level may thus provide a biological marker useful for prognosis assessment and specific therapy of the disease...
- Epithelial-mesenchymal transitions in tumour progressionJean Paul Thiery
, 144 Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
Nat Rev Cancer 2:442-54. 2002
- Mouse embryonic mammogenesis as a model for the molecular regulation of pattern formationJacqueline M Veltmaat
UMR 144 CNRS Institut Curie, Section de Recherche, Equipe de Morphogenèse Cellulaire et Progression Tumorale, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Differentiation 71:1-17. 2003..For future investigations in the field, such a unique molecular identity of each mammary rudiment should be of critical concern...
- Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profilingMathieu Clément-Ziza
Institut Curie, Département de Transfert, 26 Rue d Ulm, F 75248, Paris Cedex 05, France
BMC Genomics 10:246. 2009..To address this issue, RNA amplification methods have been developed to generate sufficient targets from picogram amounts of total RNA to perform microarray hybridisation...
- Translating metastasis-related biomarkers to the clinic--progress and pitfallsFrançois Clément Bidard
Department of Medical Oncology, Institut Curie and Université Paris Descartes, 26 Rue d Ulm, 75005 Paris, France
Nat Rev Clin Oncol 10:169-79. 2013....
- The human tissue plasminogen activator-Cre mouse: a new tool for targeting specifically neural crest cells and their derivatives in vivoThomas Pietri
UMR 144 Compartimentation et Dynamique Cellulaire Centre National de la Recherche Scientifique et Institut Curie, 26 rue d'Ulm, 75248 Paris Cedex 05, France
Dev Biol 259:176-87. 2003..Our Ht-PA-Cre mice represent a novel model to specifically target conditional mutations in migratory NCC...
- Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skinChristine E Wong
Department of Biology, Institute of Cell Biology, Swiss Federal Institute of Technology ETH Zurich, CH-8093 Zurich, Switzerland
J Cell Biol 175:1005-15. 2006..These findings are relevant for the design of therapeutic strategies because the potential of stem and progenitor cells in vivo likely depends on their nature and origin...
- Pre-EMTing metastasis? Recapitulation of morphogenetic processes in cancerGeert Berx
Department for Molecular Biomedical Research, Molecular and Cellular Oncology, VIB, Technologiepark 927, 9052, Ghent Zwijnaarde, Belgium
Clin Exp Metastasis 24:587-97. 2007..Finally we review the recent literature concerning the mechanisms that regulate EMT in the tumour context, with a particular focus on breast cancer...
- The physiology and pathology of the EMT. Meeting on the epithelial-mesenchymal transitionHervé Acloque
Instituto de Neurociencias de Alicante, CSIC UMH, Apartado 18, San Juan de Alicante, 03550 Spain
EMBO Rep 9:322-6. 2008
- Myoepithelial cells in the control of mammary development and tumorigenesis: data from genetically modified miceMarisa M Faraldo
Institut Curie, CNRS UMR144, 26 rue d'Ulm, 75248, Paris Cedex 05, France
J Mammary Gland Biol Neoplasia 10:211-9. 2005....
- Gli3-mediated somitic Fgf10 expression gradients are required for the induction and patterning of mammary epithelium along the embryonic axesJacqueline M Veltmaat
The Saban Research Institute of Childrens Hospital Los Angeles University of Southern California, Developmental Biology Program, Los Angeles, CA 90027, USA
Development 133:2325-35. 2006..We propose that the intra-somitic Fgf10 gradient, together with ventral elongation of the somites, determines the correct dorsoventral position of mammary epithelium along the flank...
- Differential expression of beta3 integrin gene in chick and mouse cranial neural crest cellsThomas Pietri
UMR 144 Compartimentation et Dynamique Cellulaire, Centre National de la Recherche Scientifique et Institut Curie, Paris, France
Dev Dyn 227:309-13. 2003..Therefore, the difference in the beta3 integrin expression suggests that mouse and chicken cranial neural crest cells may have distinct integrin requirements during their ontogenesis...
- Identification of the mammary line in mouse by Wnt10b expressionJacqueline M Veltmaat
UMR 144 CNRS Institut Curie, Equipe de Morphogenèse Cellulaire et Progression Tumorale, Paris, France
Dev Dyn 229:349-56. 2004..To date, the formation of the mammary line expressing Wnt10b is the earliest discernible ectodermal event in murine embryonic mammary gland development...
- Profiles of the 2 INK4a gene products, p16 and p14ARF, in human reference urothelium and bladder carcinomas, according to pRb and p53 protein statusMarie Aude Le Frere-Belda
Service d Anatomie et de Cytologie Pathologiques and Oncogénèse respiratoire et uro génitale EMI Institut National de la Santé de la Recherche Medical INSERM, Centre Hospitalier Universitaire Henri Mondor, Creteil, France
Hum Pathol 35:817-24. 2004..In invasive UCs, the p16-pRb pathway, the p14(ARF)-p53 pathway, or in many cases both pathways were altered or not activated, demonstrating the involvement of these pathways in invasive bladder tumorigenesis...
- Breast cancer progression with a TwistJean Paul Thiery
Nat Med 10:777-8. 2004
- Force measurements in E-cadherin-mediated cell doublets reveal rapid adhesion strengthened by actin cytoskeleton remodeling through Rac and Cdc42Yeh-Shiu Chu
Centre National de la Recherche Scientifique-Institut Curie, Paris, France
J Cell Biol 167:1183-94. 2004..Our findings highlight the crucial roles played by Rac, Cdc42, and actin cytoskeleton dynamics in the development and regulation of strong cell adhesion, defined in terms of mechanical forces...
- Host microenvironment in breast cancer development: epithelial-mesenchymal transition in breast cancer developmentAnne Vincent-Salomon
Medical Division, Department of Tumor Biology, Institut Curie, Paris, France
Breast Cancer Res 5:101-6. 2003..DNA profiling technology is also being applied to uncover pathways that lead to a metastatic phenotype...
- Linking epithelial-mesenchymal transition to the well-known polarity protein Par6Jean Paul Thiery
CNRS UMR 144, Translational Research Department, Institut Curie, 26 rue d'Ulm, 75248 Paris, Cedex 05, France
Dev Cell 8:456-8. 2005..This new study provides further insight into the protein networks involved in topologically regulated control of epithelial cell polarity and plasticity...
- Perturbation of beta1-integrin function in involuting mammary gland results in premature dedifferentiation of secretory epithelial cellsMarisa M Faraldo
Unité Mixte Recherche 144, Centre National de la Recherche Scientifique Institut Curie, Section de Recherche, 75248 Paris, France
Mol Biol Cell 13:3521-31. 2002....
- Activating mutations of the tyrosine kinase receptor FGFR3 are associated with benign skin tumors in mice and humansArmelle Logie
UMR 144, Centre National de la Recherche Scientifique, Section de Recherche, Institut Curie, Paris, France
Hum Mol Genet 14:1153-60. 2005..Our findings directly implicate FGFR3 activation as a major cause of benign epidermal tumors in humans...
- Absence of FGFR3 mutations in urinary bladder tumours of rats and mice treated with N-butyl-N-(-4-hydroxybutyl)nitrosamineClaire Dunois-Lardé
Laboratoire de Morphogenèse Cellulaire et Progression Tumorale, UMR 144, Centre National de la Recherche Scientifique, Institut Curie, Section de Recherche, Paris, France
Mol Carcinog 42:142-9. 2005..The absence of FGFR3 mutations in the rat bladder tumours suggests that, at least at the genetic level, rat superficial papillary tumours differ from their human counterparts...
- Changes in cholesterol levels in the plasma membrane modulate cell signaling and regulate cell adhesion and migration on fibronectinO G Ramprasad
Center for Cellular and Molecular Biology, Hyderabad, India
Cell Motil Cytoskeleton 64:199-216. 2007..Thus, membrane cholesterol content has a specific effect on certain signaling pathways specifically involved in regulating cell motility on fibronectin and organization of the actin cytoskeleton...
- Role of FGF10/FGFR2b signaling during mammary gland development in the mouse embryoArnaud André Mailleux
UMR144 CNRS Institut Curie, 26 rue d Ulm 75248 Paris Cedex 05, France
Development 129:53-60. 2002..Our results also suggest that FGF signaling is involved in the maintenance of mammary bud 4, and that Fgf10 deficient epithelium can undergo branching morphogenesis into the mammary fat pad precursor...
- Differential regulation of the lateral mobility of plasma membrane phospholipids by the extracellular matrix and cholesterolO G Ramprasad
Center for Cellular and Molecular Biology, Hyderabad, India
J Cell Physiol 215:550-61. 2008..Our results outline a new role of integrin-matrix interactions in the regulation of membrane phospholipid behavior...
- Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular domainsYeh Shiu Chu
UMR 144 CNRS Institut Curie, 75248 Paris, France
J Biol Chem 281:2901-10. 2006....
- The importance of being a myoepithelial cellMarie-Ange Deugnier
UMR 144 CNRS-Institut Curie, Section de Recherche, Paris, France
Breast Cancer Res 4:224-30. 2002..These functions include their effects on luminal cell growth and differentiation, their key role in the establishment of the polarised mammary epithelial bilayer and the control of stromal invasion in breast cancer...
- Growth, differentiation and senescence of normal human urothelium in an organ-like cultureAhmad Daher
INSERM EMI 03-37, , 8, , , , France
Eur Urol 45:799-805. 2004..CONCLUSIONS: This model retains many characteristics of the urothelium in vivo. It can be used for pharmacological studies between 15 to 25 days and to study mechanisms such as wound healing, proliferation and senescence...