Simon Scheuring

Summary

Affiliation: Institut Curie
Country: France

Publications

  1. ncbi request reprint Chromatic adaptation of photosynthetic membranes
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Science 309:484-7. 2005
  2. ncbi request reprint Structural models of the supramolecular organization of AQP0 and connexons in junctional microdomains
    Simon Scheuring
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    J Struct Biol 160:385-94. 2007
  3. ncbi request reprint Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopy
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche CNRS 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
    J Biol Chem 280:1426-31. 2005
  4. pmc Variable LH2 stoichiometry and core clustering in native membranes of Rhodospirillum photometricum
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, Paris, France
    EMBO J 23:4127-33. 2004
  5. pmc Watching the photosynthetic apparatus in native membranes
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
    Proc Natl Acad Sci U S A 101:11293-7. 2004
  6. ncbi request reprint The photosynthetic apparatus of Rhodopseudomonas palustris: structures and organization
    Simon Scheuring
    Institut Curie, UMR CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Mol Biol 358:83-96. 2006
  7. pmc Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopy
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 100:1690-3. 2003
  8. ncbi request reprint AFM characterization of tilt and intrinsic flexibility of Rhodobacter sphaeroides light harvesting complex 2 (LH2)
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Mol Biol 325:569-80. 2003
  9. ncbi request reprint AFM studies of the supramolecular assembly of bacterial photosynthetic core-complexes
    Simon Scheuring
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, Paris, France
    Curr Opin Chem Biol 10:387-93. 2006
  10. ncbi request reprint From high-resolution AFM topographs to atomic models of supramolecular assemblies
    Simon Scheuring
    Institut Curie, UMR168 CNRS, Paris, France
    J Struct Biol 159:268-76. 2007

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Chromatic adaptation of photosynthetic membranes
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Science 309:484-7. 2005
    ..Such structural adaptation ensures efficient photon capture under low-light conditions and prevents photodamage under high-light conditions...
  2. ncbi request reprint Structural models of the supramolecular organization of AQP0 and connexons in junctional microdomains
    Simon Scheuring
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    J Struct Biol 160:385-94. 2007
    ..Based on our AFM observations, we propose a mechanism that may explain the supramolecular organization of AQP0 and connexons in junctional domains in native lens membranes...
  3. ncbi request reprint Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopy
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche CNRS 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
    J Biol Chem 280:1426-31. 2005
    ..The QB site is located facing a approximately 25-A wide gap within LH1, explaining the PufX-favored quinone passage in and out of the core complex...
  4. pmc Variable LH2 stoichiometry and core clustering in native membranes of Rhodospirillum photometricum
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, Paris, France
    EMBO J 23:4127-33. 2004
    ..The functional significance of all these findings is discussed...
  5. pmc Watching the photosynthetic apparatus in native membranes
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
    Proc Natl Acad Sci U S A 101:11293-7. 2004
    ..The functional significance of the observed distribution is discussed...
  6. ncbi request reprint The photosynthetic apparatus of Rhodopseudomonas palustris: structures and organization
    Simon Scheuring
    Institut Curie, UMR CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Mol Biol 358:83-96. 2006
    ..The localization of the W-subunit is not restricted to the periapsis of the core-complex but randomly located with respect to the RC imposed axis...
  7. pmc Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopy
    Simon Scheuring
    Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Proc Natl Acad Sci U S A 100:1690-3. 2003
    ..The LH1 subunits rearrange into a circle after removal of the RC from the core complex...
  8. ncbi request reprint AFM characterization of tilt and intrinsic flexibility of Rhodobacter sphaeroides light harvesting complex 2 (LH2)
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Mol Biol 325:569-80. 2003
    ....
  9. ncbi request reprint AFM studies of the supramolecular assembly of bacterial photosynthetic core-complexes
    Simon Scheuring
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, Paris, France
    Curr Opin Chem Biol 10:387-93. 2006
    ..High-resolution AFM of native membranes has revealed variation in core-complex architectures amongst species...
  10. ncbi request reprint From high-resolution AFM topographs to atomic models of supramolecular assemblies
    Simon Scheuring
    Institut Curie, UMR168 CNRS, Paris, France
    J Struct Biol 159:268-76. 2007
    ..Membrane protein assemblies of megadalton size comprising several hundreds of polypeptide chains and pigments were built with Angstrom precision...
  11. doi request reprint Atomic force microscopy of the bacterial photosynthetic apparatus: plain pictures of an elaborate machinery
    Simon Scheuring
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    Photosynth Res 102:197-211. 2009
    ..These conclusions rely on the possibility that the AFM brings of obtaining molecular resolution images of large membrane areas and thereby bridging the resolution gap between atomic structures and cellular ultrastructure...
  12. ncbi request reprint Structural role of PufX in the dimerization of the photosynthetic core complex of Rhodobacter sphaeroides
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Biol Chem 279:3620-6. 2004
    ....
  13. ncbi request reprint Watching the components of photosynthetic bacterial membranes and their in situ organisation by atomic force microscopy
    Simon Scheuring
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    Biochim Biophys Acta 1712:109-27. 2005
    ....
  14. doi request reprint Native architecture of the photosynthetic membrane from Rhodobacter veldkampii
    Lu Ning Liu
    Institut Curie, U1006 INSERM, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    J Struct Biol 173:138-45. 2011
    ..veldkampii chromatophores (Rb. veldkampii features equally small vesicular chromatophores alike Rb. sphaeroides) is not due to membrane bending induced by dimeric RC-LH1-PufX cores, as it has been proposed in Rb. sphaeroides...
  15. pmc Forces guiding assembly of light-harvesting complex 2 in native membranes
    Lu Ning Liu
    Institut Curie, U1006 Institut National de la Santé et Recherche Médicale, Paris, F 75248 France
    Proc Natl Acad Sci U S A 108:9455-9. 2011
    ..In the two-step unfolding process, the β-polypeptide is stabilized by the molecular environment in the membrane. Hence, intermolecular forces influence the structural and functional integrity of LH2...
  16. ncbi request reprint Membrane insertion of Rhodopseudomonas acidophila light harvesting complex 2 investigated by high resolution AFM
    Rui Pedro Gonçalves
    Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Struct Biol 149:79-86. 2005
    ..Therefore, we present strong evidence that the tilt of LH2 does not represent the native membrane state and is due to protein-protein contacts in specific 2D-crystals...
  17. pmc The supramolecular architecture of junctional microdomains in native lens membranes
    Nikolay Buzhynskyy
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    EMBO Rep 8:51-5. 2007
    ..Finally, time-lapse AFM imaging provided insights into AQP0 array formation. This first high-resolution view of a multicomponent eukaryotic membrane shows how membrane proteins self-assemble into functional microdomains...
  18. ncbi request reprint Architecture of the native photosynthetic apparatus of Phaeospirillum molischianum
    Rui Pedro Gonçalves
    Institut Curie, UMR CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
    J Struct Biol 152:221-8. 2005
    ..blasticus, and Blastochloris viridis...
  19. doi request reprint Malformation of junctional microdomains in cataract lens membranes from a type II diabetes patient
    Stéphanie Mangenot
    Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Pflugers Arch 457:1265-74. 2009
    ..In the diabetic cataract lens, connexons were degraded, and AQP0 arrays are malformed. We conceptualize that absence of connexons lead to breakdown of cell nutrition...
  20. pmc Rows of ATP synthase dimers in native mitochondrial inner membranes
    Nikolay Buzhynskyy
    Institut Curie, UMR168 CNRS, 75248 Paris, France
    Biophys J 93:2870-6. 2007
    ..Rotation torque compensation within ATP synthase dimers stabilizes the ATP synthase structure, in particular the stator-rotor interaction...
  21. doi request reprint Mechanical mapping of single membrane proteins at submolecular resolution
    Felix Rico
    Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
    Nano Lett 11:3983-6. 2011
    ....
  22. pmc Structural information, resolution, and noise in high-resolution atomic force microscopy topographs
    Peter Fechner
    Institut Curie, Equipe Institut National de la Sante et de la Recherche Médicale Avenir, 75248 Paris, France
    Biophys J 96:3822-31. 2009
    ..Importantly, we highlight that the best single-molecule images are more accurate molecular representations than ensemble averages, because averaging downsizes the z-dimension and "blurs" structural details...
  23. ncbi request reprint Human cataract lens membrane at subnanometer resolution
    Nikolay Buzhynskyy
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    J Mol Biol 374:162-9. 2007
    ....
  24. pmc Dynamics and diffusion in photosynthetic membranes from rhodospirillum photometricum
    Simon Scheuring
    Institut Curie, Unite Mixte de Recherche Centre National de Recherche Scientifique 168, 75231 Paris Cedex 05, France
    Biophys J 91:3707-17. 2006
    ..We show that the formation of large static antennae domains may represent a strategy for increasing electron transfer rates between distant complexes within the membrane and thus be important for photosynthetic efficiency...
  25. ncbi request reprint Supramolecular assembly of VDAC in native mitochondrial outer membranes
    Rui Pedro Gonçalves
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 5, France
    J Mol Biol 369:413-8. 2007
    ..The implication of VDAC in cytochrome c release from the mitochondria during cell apoptosis has made it a target in cancer research...
  26. doi request reprint Quinone pathways in entire photosynthetic chromatophores of Rhodospirillum photometricum
    Lu Ning Liu
    Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    J Mol Biol 393:27-35. 2009
    ..These long-range quinone pathway networks result from cooperative short-range interactions of cores with their immediate environment...
  27. doi request reprint High-resolution architecture of the outer membrane of the Gram-negative bacteria Roseobacter denitrificans
    Szymon Jarosławski
    Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Mol Microbiol 74:1211-22. 2009
    ..Finally, we present first structural data of the outer membrane porin from the marine Gram-negative bacteria Roseobacter denitrificans, and we perform a sequence alignment with porins of known structure...
  28. ncbi request reprint Two-chamber AFM: probing membrane proteins separating two aqueous compartments
    Rui Pedro Gonçalves
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    Nat Methods 3:1007-12. 2006
    ..We combined AFM and fluorescence microscopy to demonstrate the functionality of proteins in the setup by documenting proton pumping by Halobacterium salinarium purple membranes...
  29. doi request reprint Two-dimensional kinetics of inter-connexin interactions from single-molecule force spectroscopy
    Felix Rico
    Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
    J Mol Biol 412:72-9. 2011
    ..Our results propose the gap junction channel as an adhesion complex that associates slowly and dissociates fast at low force but is able to support important pulling forces in its native, hexameric form...
  30. ncbi request reprint Direct visualization of KirBac3.1 potassium channel gating by atomic force microscopy
    Szymon Jarosławski
    Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
    J Mol Biol 374:500-5. 2007
    ..This is the first time AFM has been used as an interactive tool to study K(+) channels. It has enabled us to directly measure the conformational changes in the protein surface produced by ligand binding...
  31. doi request reprint Rhodopsin is spatially heterogeneously distributed in rod outer segment disk membranes
    Nikolay Buzhynskyy
    Institut Curie, U1006INSERM, 26rued Ulm, 75005 Paris, France
    J Mol Recognit 24:483-9. 2011
    ..The described supramolecular assembly of rhodospin, peripherin/Rom and lipids in native rod outer segment disks is consistent with the functional requirements of photoreception...
  32. doi request reprint Automated setpoint adjustment for biological contact mode atomic force microscopy imaging
    Ignacio Casuso
    Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, Paris, France
    Nanotechnology 21:035104. 2010
    ..A system that permanently performs this methodology steered the AFM towards high resolution imaging forces and imaged purple membrane at molecular resolution and live cells at high signal-to-noise ratio for hours without an operator...
  33. doi request reprint High-speed atomic force microscopy: Structure and dynamics of single proteins
    Ignacio Casuso
    INSERM U1006, Institut Curie, 26 Rue d Ulm, 75005 Paris, France
    Curr Opin Chem Biol 15:704-9. 2011
    ..From this imaging data, watching molecules at work, novel and direct insights could be gained concerning the structure, dynamics and function relationship at the single bio-molecule level...
  34. doi request reprint Atomic force microscopy: probing the spatial organization, interactions and elasticity of microbial cell envelopes at molecular resolution
    Simon Scheuring
    Institut Curie, Centre de Recherche, Paris, France
    Mol Microbiol 75:1327-36. 2010
    ..These nanoscale experiments complement microscopy, genetics and biochemical methods traditionally used to analyse the microbial envelope...
  35. pmc Cellular capsules as a tool for multicellular spheroid production and for investigating the mechanics of tumor progression in vitro
    Kevin Alessandri
    Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 110:14843-8. 2013
    ..Overall, our technology offers a unique avenue to produce in vitro cell-based assays useful for developing new anticancer therapies and to investigate the interplay between mechanics and growth in tumor evolution. ..
  36. pmc Contact-mode high-resolution high-speed atomic force microscopy movies of the purple membrane
    Ignacio Casuso
    Institut Curie, Equipe Institut National de la Sante et de la Recherche Médicale Avenir, Unite Mixte de Recherche 168 Centre National de la Recherche Scientifique, Paris, France
    Biophys J 97:1354-61. 2009
    ..Our findings indicate that the bR arrays are in a mobile association-dissociation equilibrium. HS-AFM on membranes provides novel perspectives for analyzing the membrane diffusion processes of nonlabeled molecules...
  37. pmc Direct measurement of the mechanical properties of lipid phases in supported bilayers
    Laura Picas
    Biophys J 102:L01-3. 2012
    ..3 MPa and 28.1 MPa allowed us to estimate the area stretch modulus (k(A)) as 106 pN/nm and 199 pN/nm and the bending stiffness (k(c)) as 18 k(B)T and 57 k(B)T for the liquid and gel phases, respectively...
  38. doi request reprint Biological AFM: where we come from--where we are--where we may go
    Ignacio Casuso
    Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
    J Mol Recognit 24:406-13. 2011
    ..Of all these topics, particular representative examples are shown, each of them standing for a variety of achievements by many research groups...
  39. pmc Carbohydrate-carbohydrate interaction provides adhesion force and specificity for cellular recognition
    Iwona Bucior
    Friedrich Miescher Institute, 4058 Basel, Switzerland
    J Cell Biol 165:529-37. 2004
    ..These findings confirm for the first time the existence of relatively strong and species-specific recognition between surface glycans, a process that may have significant implications in cellular recognition...
  40. ncbi request reprint The 4.5 A structure of human AQP2
    Andreas D Schenk
    M E Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    J Mol Biol 350:278-89. 2005
    ..The structure determined to 4.5 A resolution in the plane of the membrane reveals the typical aquaporin fold but also a particular structure between the stacked layers that is likely to be related to the cytosolic N and C termini...
  41. ncbi request reprint Sampling the conformational space of membrane protein surfaces with the AFM
    Simon Scheuring
    M E Müller Institute for Microscopy at the Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
    Eur Biophys J 31:172-8. 2002
    ..Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00249-001-0197-8..
  42. doi request reprint Introduction to atomic force microscopy (AFM) in biology
    Claire Goldsbury
    Cytoskeleton Group, Max Planck Unit for Structural Molecular Biology, Hamburg, Germany
    Curr Protoc Protein Sci . 2002
    ..The physical principles of the technique and methodological aspects of its practical use and applications are also described...
  43. ncbi request reprint Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope
    Simon Scheuring
    M E Müller Institute for Microscopy at the Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056, Switzerland
    Mol Microbiol 44:675-84. 2002
    ..The results provide a basis for understanding the amazing stability of this protective bacterial surface coat...
  44. ncbi request reprint Past, present and future of atomic force microscopy in life sciences and medicine
    Pierre Parot
    CEA Valrhô DSV iBEB SBTN, 30207 Bagnols sur Cèze Marcoule, France
    J Mol Recognit 20:418-31. 2007
    ..Future prospects in these different fields are also highlighted...
  45. ncbi request reprint Imaging and manipulation of biological structures with the AFM
    Dimitrios Fotiadis
    M E Müller Institute for Microscopy, Biozentrum of the University of Basel, CH 4056 Basel, Switzerland
    Micron 33:385-97. 2002
    ..Future application of these nanotechniques will reveal new information on the structure, function and assembly of biomolecules...
  46. ncbi request reprint Single-molecule studies of membrane proteins
    Daniel J Muller
    Center for Biotechnology, University of Technology, 01307 Dresden, Germany
    Curr Opin Struct Biol 16:489-95. 2006
    ..These interactions dictate the stability of the protein and might be modulated by ligands that alter the protein's functional state...