Research Topics
Genomes and GenesSpecies | Simon ScheuringSummaryAffiliation: Institut Curie Country: France Publications
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Publications
Chromatic adaptation of photosynthetic membranesSimon Scheuring
Institut Curie, Unité Mixte de Recherche CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
Science 309:484-7. 2005..Such structural adaptation ensures efficient photon capture under low-light conditions and prevents photodamage under high-light conditions...- The photosynthetic apparatus of Rhodopseudomonas palustris: structures and organizationSimon Scheuring
Institut Curie, UMR CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
J Mol Biol 358:83-96. 2006..The localization of the W-subunit is not restricted to the periapsis of the core-complex but randomly located with respect to the RC imposed axis... - Structure of the dimeric PufX-containing core complex of Rhodobacter blasticus by in situ atomic force microscopySimon Scheuring
Institut Curie, Unité Mixte de Recherche CNRS 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
J Biol Chem 280:1426-31. 2005..The QB site is located facing a approximately 25-A wide gap within LH1, explaining the PufX-favored quinone passage in and out of the core complex... 
Variable LH2 stoichiometry and core clustering in native membranes of Rhodospirillum photometricumSimon Scheuring
Institut Curie, UMR CNRS 168 and LRC CEA 34V, Paris, France
EMBO J 23:4127-33. 2004..The functional significance of all these findings is discussed...- Watching the photosynthetic apparatus in native membranesSimon Scheuring
Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris 05, France
Proc Natl Acad Sci U S A 101:11293-7. 2004..The functional significance of the observed distribution is discussed... - AFM studies of the supramolecular assembly of bacterial photosynthetic core-complexesSimon Scheuring
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, Paris, France
Curr Opin Chem Biol 10:387-93. 2006..High-resolution AFM of native membranes has revealed variation in core-complex architectures amongst species... 
Atomic force microscopy of the bacterial photosynthetic apparatus: plain pictures of an elaborate machinerySimon Scheuring
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
Photosynth Res 102:197-211. 2009..These conclusions rely on the possibility that the AFM brings of obtaining molecular resolution images of large membrane areas and thereby bridging the resolution gap between atomic structures and cellular ultrastructure...- Nanodissection and high-resolution imaging of the Rhodopseudomonas viridis photosynthetic core complex in native membranes by AFM. Atomic force microscopySimon Scheuring
Institut Curie, Unité Mixte de Recherche Centre National de la Recherche Scientifique 168 and Laboratoire de Recherche Correspondant Commissariat à l Energie Atomique 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
Proc Natl Acad Sci U S A 100:1690-3. 2003..The LH1 subunits rearrange into a circle after removal of the RC from the core complex... - AFM characterization of tilt and intrinsic flexibility of Rhodobacter sphaeroides light harvesting complex 2 (LH2)Simon Scheuring
Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
J Mol Biol 325:569-80. 2003.... - From high-resolution AFM topographs to atomic models of supramolecular assembliesSimon Scheuring
Institut Curie, UMR168 CNRS, Paris, France
J Struct Biol 159:268-76. 2007..Membrane protein assemblies of megadalton size comprising several hundreds of polypeptide chains and pigments were built with Angstrom precision... - Structural models of the supramolecular organization of AQP0 and connexons in junctional microdomainsSimon Scheuring
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
J Struct Biol 160:385-94. 2007..Based on our AFM observations, we propose a mechanism that may explain the supramolecular organization of AQP0 and connexons in junctional domains in native lens membranes... - Structural role of PufX in the dimerization of the photosynthetic core complex of Rhodobacter sphaeroidesSimon Scheuring
Institut Curie, UMR-CNRS 168 and LRC-CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
J Biol Chem 279:3620-6. 2004.... - Watching the components of photosynthetic bacterial membranes and their in situ organisation by atomic force microscopySimon Scheuring
Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
Biochim Biophys Acta 1712:109-27. 2005.... - Membrane insertion of Rhodopseudomonas acidophila light harvesting complex 2 investigated by high resolution AFMRui Pedro Gonçalves
Institut Curie, UMR CNRS 168 and LRC CEA 34V, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
J Struct Biol 149:79-86. 2005..Therefore, we present strong evidence that the tilt of LH2 does not represent the native membrane state and is due to protein-protein contacts in specific 2D-crystals... - Native architecture of the photosynthetic membrane from Rhodobacter veldkampiiLu Ning Liu
Institut Curie, U1006 INSERM, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
J Struct Biol 173:138-45. 2011..veldkampii chromatophores (Rb. veldkampii features equally small vesicular chromatophores alike Rb. sphaeroides) is not due to membrane bending induced by dimeric RC-LH1-PufX cores, as it has been proposed in Rb. sphaeroides... - Forces guiding assembly of light-harvesting complex 2 in native membranesLu Ning Liu
Institut Curie, U1006 Institut National de la Santé et Recherche Médicale, Paris, F 75248 France
Proc Natl Acad Sci U S A 108:9455-9. 2011..In the two-step unfolding process, the β-polypeptide is stabilized by the molecular environment in the membrane. Hence, intermolecular forces influence the structural and functional integrity of LH2... - The supramolecular architecture of junctional microdomains in native lens membranesNikolay Buzhynskyy
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
EMBO Rep 8:51-5. 2007..Finally, time-lapse AFM imaging provided insights into AQP0 array formation. This first high-resolution view of a multicomponent eukaryotic membrane shows how membrane proteins self-assemble into functional microdomains... - Architecture of the native photosynthetic apparatus of Phaeospirillum molischianumRui Pedro Gonçalves
Institut Curie, UMR CNRS 168, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France
J Struct Biol 152:221-8. 2005..blasticus, and Blastochloris viridis... - Mechanical mapping of single membrane proteins at submolecular resolutionFelix Rico
Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
Nano Lett 11:3983-6. 2011.... - Structural information, resolution, and noise in high-resolution atomic force microscopy topographsPeter Fechner
Institut Curie, Equipe Institut National de la Sante et de la Recherche Médicale Avenir, 75248 Paris, France
Biophys J 96:3822-31. 2009..Importantly, we highlight that the best single-molecule images are more accurate molecular representations than ensemble averages, because averaging downsizes the z-dimension and "blurs" structural details... - Rows of ATP synthase dimers in native mitochondrial inner membranesNikolay Buzhynskyy
Institut Curie, UMR168 CNRS, 75248 Paris, France
Biophys J 93:2870-6. 2007..Rotation torque compensation within ATP synthase dimers stabilizes the ATP synthase structure, in particular the stator-rotor interaction... - Malformation of junctional microdomains in cataract lens membranes from a type II diabetes patientStéphanie Mangenot
Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Pflugers Arch 457:1265-74. 2009..In the diabetic cataract lens, connexons were degraded, and AQP0 arrays are malformed. We conceptualize that absence of connexons lead to breakdown of cell nutrition... - Human cataract lens membrane at subnanometer resolutionNikolay Buzhynskyy
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Mol Biol 374:162-9. 2007.... - Dynamics and diffusion in photosynthetic membranes from rhodospirillum photometricumSimon Scheuring
Institut Curie, Unite Mixte de Recherche Centre National de Recherche Scientifique 168, 75231 Paris Cedex 05, France
Biophys J 91:3707-17. 2006..We show that the formation of large static antennae domains may represent a strategy for increasing electron transfer rates between distant complexes within the membrane and thus be important for photosynthetic efficiency... - High-resolution architecture of the outer membrane of the Gram-negative bacteria Roseobacter denitrificansSzymon Jarosławski
Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
Mol Microbiol 74:1211-22. 2009..Finally, we present first structural data of the outer membrane porin from the marine Gram-negative bacteria Roseobacter denitrificans, and we perform a sequence alignment with porins of known structure... - Quinone pathways in entire photosynthetic chromatophores of Rhodospirillum photometricumLu Ning Liu
Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 05, France
J Mol Biol 393:27-35. 2009..These long-range quinone pathway networks result from cooperative short-range interactions of cores with their immediate environment... - Supramolecular assembly of VDAC in native mitochondrial outer membranesRui Pedro Gonçalves
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris Cedex 5, France
J Mol Biol 369:413-8. 2007..The implication of VDAC in cytochrome c release from the mitochondria during cell apoptosis has made it a target in cancer research... - Two-chamber AFM: probing membrane proteins separating two aqueous compartmentsRui Pedro Gonçalves
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
Nat Methods 3:1007-12. 2006..We combined AFM and fluorescence microscopy to demonstrate the functionality of proteins in the setup by documenting proton pumping by Halobacterium salinarium purple membranes... - Direct visualization of KirBac3.1 potassium channel gating by atomic force microscopySzymon Jarosławski
Institut Curie, UMR168 CNRS, 26 Rue d Ulm, 75248 Paris, France
J Mol Biol 374:500-5. 2007..This is the first time AFM has been used as an interactive tool to study K(+) channels. It has enabled us to directly measure the conformational changes in the protein surface produced by ligand binding... - Two-dimensional kinetics of inter-connexin interactions from single-molecule force spectroscopyFelix Rico
Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
J Mol Biol 412:72-9. 2011..Our results propose the gap junction channel as an adhesion complex that associates slowly and dissociates fast at low force but is able to support important pulling forces in its native, hexameric form... - Rhodopsin is spatially heterogeneously distributed in rod outer segment disk membranesNikolay Buzhynskyy
Institut Curie, U1006INSERM, 26rued Ulm, 75005 Paris, France
J Mol Recognit 24:483-9. 2011..The described supramolecular assembly of rhodospin, peripherin/Rom and lipids in native rod outer segment disks is consistent with the functional requirements of photoreception... - Automated setpoint adjustment for biological contact mode atomic force microscopy imagingIgnacio Casuso
Institut Curie, Equipe INSERM AVENIR, UMR168 CNRS, Paris, France
Nanotechnology 21:035104. 2010..A system that permanently performs this methodology steered the AFM towards high resolution imaging forces and imaged purple membrane at molecular resolution and live cells at high signal-to-noise ratio for hours without an operator... - High-speed atomic force microscopy: Structure and dynamics of single proteinsIgnacio Casuso
INSERM U1006, Institut Curie, 26 Rue d Ulm, 75005 Paris, France
Curr Opin Chem Biol 15:704-9. 2011..From this imaging data, watching molecules at work, novel and direct insights could be gained concerning the structure, dynamics and function relationship at the single bio-molecule level... - Atomic force microscopy: probing the spatial organization, interactions and elasticity of microbial cell envelopes at molecular resolutionSimon Scheuring
Institut Curie, Centre de Recherche, Paris, France
Mol Microbiol 75:1327-36. 2010..These nanoscale experiments complement microscopy, genetics and biochemical methods traditionally used to analyse the microbial envelope... - Contact-mode high-resolution high-speed atomic force microscopy movies of the purple membraneIgnacio Casuso
Institut Curie, Equipe Institut National de la Sante et de la Recherche Médicale Avenir, Unite Mixte de Recherche 168 Centre National de la Recherche Scientifique, Paris, France
Biophys J 97:1354-61. 2009..Our findings indicate that the bR arrays are in a mobile association-dissociation equilibrium. HS-AFM on membranes provides novel perspectives for analyzing the membrane diffusion processes of nonlabeled molecules... - Direct measurement of the mechanical properties of lipid phases in supported bilayersLaura Picas
Biophys J 102:L01-3. 2012..3 MPa and 28.1 MPa allowed us to estimate the area stretch modulus (k(A)) as 106 pN/nm and 199 pN/nm and the bending stiffness (k(c)) as 18 k(B)T and 57 k(B)T for the liquid and gel phases, respectively... - Biological AFM: where we come from--where we are--where we may goIgnacio Casuso
Institut Curie, U1006 INSERM, 26 Rue d Ulm, 75005 Paris, France
J Mol Recognit 24:406-13. 2011..Of all these topics, particular representative examples are shown, each of them standing for a variety of achievements by many research groups... 
Carbohydrate-carbohydrate interaction provides adhesion force and specificity for cellular recognitionIwona Bucior
Friedrich Miescher Institute, 4058 Basel, Switzerland
J Cell Biol 165:529-37. 2004..These findings confirm for the first time the existence of relatively strong and species-specific recognition between surface glycans, a process that may have significant implications in cellular recognition...- Sampling the conformational space of membrane protein surfaces with the AFMSimon Scheuring
M E Müller Institute for Microscopy at the Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
Eur Biophys J 31:172-8. 2002..Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00249-001-0197-8.. - The 4.5 A structure of human AQP2Andreas D Schenk
M E Müller Institute for Microscopy, Biozentrum, University of Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland
J Mol Biol 350:278-89. 2005..The structure determined to 4.5 A resolution in the plane of the membrane reveals the typical aquaporin fold but also a particular structure between the stacked layers that is likely to be related to the cytosolic N and C termini... - Single-molecule studies of membrane proteinsDaniel J Muller
Center for Biotechnology, University of Technology, 01307 Dresden, Germany
Curr Opin Struct Biol 16:489-95. 2006..These interactions dictate the stability of the protein and might be modulated by ligands that alter the protein's functional state... - Introduction to atomic force microscopy (AFM) in biologyClaire Goldsbury
Cytoskeleton Group, Max Planck Unit for Structural Molecular Biology, Hamburg, Germany
Curr Protoc Protein Sci . 2002..The physical principles of the technique and methodological aspects of its practical use and applications are also described... - Imaging and manipulation of biological structures with the AFMDimitrios Fotiadis
, Biozentrum of the University of Basel, CH-4056 Basel, Switzerland
Micron 33:385-97. 2002..Future application of these nanotechniques will reveal new information on the structure, function and assembly of biomolecules... - Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscopeSimon Scheuring
, University of Basel, Klingelbergstrasse 70, CH-4056, Switzerland
Mol Microbiol 44:675-84. 2002..The results provide a basis for understanding the amazing stability of this protective bacterial surface coat... - Past, present and future of atomic force microscopy in life sciences and medicinePierre Parot
CEA Valrhô DSV iBEB SBTN, 30207 Bagnols sur Cèze Marcoule, France
J Mol Recognit 20:418-31. 2007..Future prospects in these different fields are also highlighted...