Bertrand Raynal

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. doi request reprint Quality assessment and optimization of purified protein samples: why and how?
    Bertrand Raynal
    Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France
    Microb Cell Fact 13:180. 2014
  2. pmc Hydrodynamic characterization of recombinant human fibrinogen species
    Bertrand Raynal
    Institut Pasteur, Protéopole, Plate forme de Biophysique des Macromolecules et de leurs interactions, CNRS UMR 3528, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Thromb Res 132:e48-53. 2013
  3. doi request reprint Biochemical and biophysical characterisation of DBL1alpha1-varO, the rosetting domain of PfEMP1 from the VarO line of Plasmodium falciparum
    Alexandre Juillerat
    Unité d Immunologie Structurale, CNRS URA 2185, Institut Pasteur, 25 28 Rue du Dr Roux, F 75015 Paris, France
    Mol Biochem Parasitol 170:84-92. 2010
  4. pmc Structural basis for the ABO blood-group dependence of Plasmodium falciparum rosetting
    Inès Vigan-Womas
    Institut Pasteur, Unité d Immunologie Moléculaire des Parasites, Paris, France
    PLoS Pathog 8:e1002781. 2012
  5. pmc The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells
    Doranda Perdomo
    Institut Pasteur, Cell Biology of Parasitism Unit, F 75015 Paris, France
    Sci Rep 3:1171. 2013
  6. doi request reprint DARPin-assisted crystallography of the CC2-LZ domain of NEMO reveals a coupling between dimerization and ubiquitin binding
    Olivera Grubisha
    Unité de Biochimie Structurale et Cellulaire, Institut Pasteur, CNRS, URA 2185, Paris, France
    J Mol Biol 395:89-104. 2010
  7. pmc Outer membrane targeting of secretin PulD protein relies on disordered domain recognition by a dedicated chaperone
    Nicholas N Nickerson
    Institut Pasteur, Molecular Genetics Unit, Microbiology Department, rue du Dr Roux, 75015 Paris, France
    J Biol Chem 286:38833-43. 2011
  8. doi request reprint Comparative structural studies of two natural isoforms of ammodytoxin, phospholipases A2 from Vipera ammodytes ammodytes which differ in neurotoxicity and anticoagulant activity
    Frederick A Saul
    Institut Pasteur, Unité d Immunologie Structurale, CNRS, URA 2185, Departement de Biologie Structurale et Chimie, F 75015 Paris, France
    J Struct Biol 169:360-9. 2010
  9. pmc Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR
    Idir Malki
    Institut Pasteur, Unité de RMN des Biomolécules, Departement de Biologie Structurale et Chimie, Paris, France CNRS, UMR 3528, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France
    PLoS ONE 9:e89502. 2014
  10. doi request reprint Regulation of the catalytic activity of the human phosphatase PTPN4 by its PDZ domain
    Pierre Maisonneuve
    Departement de Biologie Structurale et Chimie, Unité de Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France
    FEBS J 281:4852-65. 2014

Collaborators

Detail Information

Publications15

  1. doi request reprint Quality assessment and optimization of purified protein samples: why and how?
    Bertrand Raynal
    Institut Pasteur, Biophysics of Macromolecules and their Interactions, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France
    Microb Cell Fact 13:180. 2014
    ..Approaches are then suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency. ..
  2. pmc Hydrodynamic characterization of recombinant human fibrinogen species
    Bertrand Raynal
    Institut Pasteur, Protéopole, Plate forme de Biophysique des Macromolecules et de leurs interactions, CNRS UMR 3528, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Thromb Res 132:e48-53. 2013
    ..Recombinant production of human fibrinogen allows investigating the impact on the three-dimensional structure of specific changes in the primary structure...
  3. doi request reprint Biochemical and biophysical characterisation of DBL1alpha1-varO, the rosetting domain of PfEMP1 from the VarO line of Plasmodium falciparum
    Alexandre Juillerat
    Unité d Immunologie Structurale, CNRS URA 2185, Institut Pasteur, 25 28 Rue du Dr Roux, F 75015 Paris, France
    Mol Biochem Parasitol 170:84-92. 2010
    ..Thus high yields of soluble NTS-DBL1alpha(1) with native conformation have been produced, opening novel perspectives for both structure-function studies and vaccine development...
  4. pmc Structural basis for the ABO blood-group dependence of Plasmodium falciparum rosetting
    Inès Vigan-Womas
    Institut Pasteur, Unité d Immunologie Moléculaire des Parasites, Paris, France
    PLoS Pathog 8:e1002781. 2012
    ....
  5. pmc The α-helical regions of KERP1 are important in Entamoeba histolytica adherence to human cells
    Doranda Perdomo
    Institut Pasteur, Cell Biology of Parasitism Unit, F 75015 Paris, France
    Sci Rep 3:1171. 2013
    ..Our observations suggest a link between the inhibitory effect of the isolated central region and the structural features of KERP1...
  6. doi request reprint DARPin-assisted crystallography of the CC2-LZ domain of NEMO reveals a coupling between dimerization and ubiquitin binding
    Olivera Grubisha
    Unité de Biochimie Structurale et Cellulaire, Institut Pasteur, CNRS, URA 2185, Paris, France
    J Mol Biol 395:89-104. 2010
    ..Collectively, these data provide structural insights into how several pathological mutations within and outside of the CC2-LZ's NOA ubiquitin binding site affect IkappaB kinase activation in the NF-kappaB signaling pathway...
  7. pmc Outer membrane targeting of secretin PulD protein relies on disordered domain recognition by a dedicated chaperone
    Nicholas N Nickerson
    Institut Pasteur, Molecular Genetics Unit, Microbiology Department, rue du Dr Roux, 75015 Paris, France
    J Biol Chem 286:38833-43. 2011
    ....
  8. doi request reprint Comparative structural studies of two natural isoforms of ammodytoxin, phospholipases A2 from Vipera ammodytes ammodytes which differ in neurotoxicity and anticoagulant activity
    Frederick A Saul
    Institut Pasteur, Unité d Immunologie Structurale, CNRS, URA 2185, Departement de Biologie Structurale et Chimie, F 75015 Paris, France
    J Struct Biol 169:360-9. 2010
    ..The crystal structure shows that the C-terminal region, important for binding to FXa and CaM, is fully exposed and accessible for interaction with proteic receptors in both the monomeric and dimeric forms of ammodytoxin described here...
  9. pmc Interaction of a partially disordered antisigma factor with its partner, the signaling domain of the TonB-dependent transporter HasR
    Idir Malki
    Institut Pasteur, Unité de RMN des Biomolécules, Departement de Biologie Structurale et Chimie, Paris, France CNRS, UMR 3528, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France
    PLoS ONE 9:e89502. 2014
    ..Their conservation in several bacteria suggests wider significance of the proposed model for the understanding of bacterial transmembrane signaling. ..
  10. doi request reprint Regulation of the catalytic activity of the human phosphatase PTPN4 by its PDZ domain
    Pierre Maisonneuve
    Departement de Biologie Structurale et Chimie, Unité de Résonance Magnétique Nucléaire des Biomolécules, Institut Pasteur, Paris, France Université Pierre et Marie Curie, Cellule Pasteur UPMC, Paris, France
    FEBS J 281:4852-65. 2014
    ..This study strengthens the emerging notion that PDZ domains can act as regulators of enzyme activity and therefore are active players in the dynamic regulation of signaling pathways...
  11. doi request reprint Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin
    Johanna C Karst
    Institut Pasteur, Unité de Biochimie des Interactions Macromoléculaires, CNRS URA 2185, Departement de Biologie Structurale et Chimie, 25 28 Rue du Dr Roux, 75724 Paris Cedex 15, France
    Biochemistry 49:318-28. 2010
    ..On the basis of these data, we propose a model for the structural transition between the calmodulin-free and calmodulin-bound AC...
  12. doi request reprint Crystal structure of the extracellular domain of a bacterial ligand-gated ion channel
    Hugues Nury
    Institut Pasteur, Unité de Dynamique Structurale des Macromolécules, CNRS URA 2185, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    J Mol Biol 395:1114-27. 2010
    ..The hexameric or pentameric forms of the ECD have a similar negative curvature in their subunit-subunit interface, while acetylcholine binding proteins have a smaller and positive curvature that increases from the apo to the holo form...
  13. pmc Full-length extracellular region of the var2CSA variant of PfEMP1 is required for specific, high-affinity binding to CSA
    Anand Srivastava
    Institut Pasteur, Unité de Biologie des Interactions Hôte Parasite, Centre National de la Recherche Scientifique Unité de Recherche Associée 2581, 25 rue du Docteur Roux, F 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 107:4884-9. 2010
    ..These results have important consequences for the development of an effective vaccine and therapeutic inhibitors...
  14. pmc Visualization of a substrate-induced productive conformation of the catalytic triad of the Neisseria meningitidis peptidoglycan O-acetylesterase reveals mechanistic conservation in SGNH esterase family members
    Allison H Williams
    Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Dept Microbiologie, 28 rue du Dr Roux, 75015 Paris, France
    Acta Crystallogr D Biol Crystallogr 70:2631-9. 2014
    ..This substrate-induced productive conformation of the catalytic triad shifts the established dogma on these enzymes, generating valuable insight into the structure-based design of drugs targeting the SGNH esterase superfamily. ..
  15. pmc Assembly and proteolytic processing of mycobacterial ClpP1 and ClpP2
    Nadia Benaroudj
    Institut Pasteur, Unité de Biologie des Spirochètes, Institut Pasteur, F 75015 Paris, France
    BMC Biochem 12:61. 2011
    ..The Mycobacterium tuberculosis (MTB) genome contains two genes coding for putative ClpPs, ClpP1 and ClpP2 respectively, that are likely to play a role in the virulence of the bacterium...