Graca Raposo

Summary

Affiliation: Institut Curie
Country: France

Publications

  1. pmc Dynamics of major histocompatibility complex class II compartments during B cell receptor-mediated cell activation
    Danielle Lankar
    Institut National de la Sante et de la Recherche Medicale U520 INSERM, Institut Curie, 12 rue Lhomond, 75005 Paris, France
    J Exp Med 195:461-72. 2002
  2. pmc Lysosome-related organelles: driving post-Golgi compartments into specialisation
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Curr Opin Cell Biol 19:394-401. 2007
  3. ncbi request reprint BCR-bound antigen is targeted to exosomes in human follicular lymphoma B-cells
    Pascale Rialland
    INSERM U520, Institut Curie, 26, rue d Ulm, 75 248 Paris cedex 05, France
    Biol Cell 98:491-501. 2006
  4. pmc Cells release prions in association with exosomes
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:9683-8. 2004
  5. doi request reprint Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network
    Claudia G Almeida
    Institut Curie, Centre de Recherche, Paris, F 75248, France
    Nat Cell Biol 13:779-89. 2011
  6. pmc Birbeck granules are subdomains of endosomal recycling compartment in human epidermal Langerhans cells, which form where Langerin accumulates
    Ray Mc Dermott
    Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, Laboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, 75248 Paris, France
    Mol Biol Cell 13:317-35. 2002
  7. pmc The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis
    Guillaume van Niel
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Dev Cell 21:708-21. 2011
  8. ncbi request reprint Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
  9. ncbi request reprint TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex
    Nicolas Blanchard
    Institut National de la Sante et de la Recherche Medicale, Unité 520, Institut Curie, Paris, France
    J Immunol 168:3235-41. 2002
  10. ncbi request reprint Exosomes: a common pathway for a specialized function
    Guillaume van Niel
    Institut Curie, CNRS UMR144, 75248 Paris Cedex, France
    J Biochem 140:13-21. 2006

Detail Information

Publications48

  1. pmc Dynamics of major histocompatibility complex class II compartments during B cell receptor-mediated cell activation
    Danielle Lankar
    Institut National de la Sante et de la Recherche Medicale U520 INSERM, Institut Curie, 12 rue Lhomond, 75005 Paris, France
    J Exp Med 195:461-72. 2002
    ..Thus, BcR engagement induced the transient formation of antigen-processing compartments, enabling antigen-specific B cells to become effective antigen-presenting cells...
  2. pmc Lysosome-related organelles: driving post-Golgi compartments into specialisation
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Curr Opin Cell Biol 19:394-401. 2007
    ..Current research reveals how the products of these genes cooperate to generate LROs and how these otherwise diverse organelles are related by the mechanisms through which they form...
  3. ncbi request reprint BCR-bound antigen is targeted to exosomes in human follicular lymphoma B-cells
    Pascale Rialland
    INSERM U520, Institut Curie, 26, rue d Ulm, 75 248 Paris cedex 05, France
    Biol Cell 98:491-501. 2006
    ..Exosomes have been well studied in EBV (Epstein-Barr virus)-transformed human B-cell lines; however, biological stimuli regulating exosome secretion quantitatively and/or qualitatively still remain poorly defined...
  4. pmc Cells release prions in association with exosomes
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Proc Natl Acad Sci U S A 101:9683-8. 2004
    ..Furthermore, we found that exosomes bearing PrPsc are infectious. Our data suggest that exosomes may contribute to intercellular membrane exchange and the spread of prions throughout the organism...
  5. doi request reprint Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network
    Claudia G Almeida
    Institut Curie, Centre de Recherche, Paris, F 75248, France
    Nat Cell Biol 13:779-89. 2011
    ..Our results provide evidence that actin and myosin 1 regulate organelle shape and uncover an important function for myosin 1b in the initiation of post-Golgi carrier formation by regulating actin assembly and remodelling TGN membranes...
  6. pmc Birbeck granules are subdomains of endosomal recycling compartment in human epidermal Langerhans cells, which form where Langerin accumulates
    Ray Mc Dermott
    Unité Mixte de Recherche 144 Centre National de la Recherche Scientifique, Laboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, 75248 Paris, France
    Mol Biol Cell 13:317-35. 2002
    ..Finally, our results implicate ADP-ribosylation factor proteins in Langerin trafficking and the exchange between Birbeck granules and other endosomal membranes...
  7. pmc The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis
    Guillaume van Niel
    Institut Curie, Centre de Recherche, F 75248 Paris, France
    Dev Cell 21:708-21. 2011
    ..Our data thus reveal a tight interplay regulated by CD63 between two distinct endosomal ILV sorting processes for a single cargo during LRO biogenesis...
  8. ncbi request reprint Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes
    Carolina Jancic
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Nat Cell Biol 9:367-78. 2007
    ..The Rab27a-dependent recruitment of these "inhibitory lysosome-related organelles" to phagosomes continuously limits acidification and degradation of ingested particles in dendritic cells, thus promoting antigen cross-presentation...
  9. ncbi request reprint TCR activation of human T cells induces the production of exosomes bearing the TCR/CD3/zeta complex
    Nicolas Blanchard
    Institut National de la Sante et de la Recherche Medicale, Unité 520, Institut Curie, Paris, France
    J Immunol 168:3235-41. 2002
    ....
  10. ncbi request reprint Exosomes: a common pathway for a specialized function
    Guillaume van Niel
    Institut Curie, CNRS UMR144, 75248 Paris Cedex, France
    J Biochem 140:13-21. 2006
    ..These findings open up new therapeutic and diagnostic strategies...
  11. doi request reprint Myosin VI regulates actin dynamics and melanosome biogenesis
    Sylvain Loubéry
    Institut Curie, Centre de Recherche, Paris, F 75248, France
    Traffic 13:665-80. 2012
    ..Together, our results provide new evidence that myosin VI regulates the organization of actin dynamics at the surface of a specialized organelle and unravel a novel function of this myosin in regulating the biogenesis of this organelle...
  12. ncbi request reprint Phosphoinositide 3-kinase activation by Igbeta controls de novo formation of an antigen-processing compartment
    Marie Granboulan
    INSERM U520, UMR 144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    J Biol Chem 278:4331-8. 2003
    ..Altogether, these results strongly suggest that Igbeta couples the BCR to PI3K activation that is instrumental for de novo formation of the antigen processing compartment and efficient antigen presentation...
  13. pmc Human immunodeficiency virus-1 Nef expression induces intracellular accumulation of multivesicular bodies and major histocompatibility complex class II complexes: potential role of phosphatidylinositol 3-kinase
    Pamela Stumptner-Cuvelette
    Institut National de la Santé et de la Recherche Médicale U520, Institut Curie, Section de recherche 75005 Paris, France
    Mol Biol Cell 14:4857-70. 2003
    ..Given that human immunodeficiency virus recruits the MVB machinery for its assembly process, our data raise the possibility that Nef is involved in viral assembly through its effect on MVBs...
  14. pmc BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells
    Leila Rochin
    Institut Curie, and Unité Mixte de Recherche 144, Centre National de la Recherche Scientifique, F 75248 Paris, France
    Proc Natl Acad Sci U S A 110:10658-63. 2013
    ....
  15. doi request reprint Inhibition of retrograde transport protects mice from lethal ricin challenge
    Bahne Stechmann
    Traffic, Signaling, and Delivery Laboratory, Centre de Recherche, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Cell 141:231-42. 2010
    ..Our work discovers the first small molecule that shows efficacy against ricin in animal experiments and identifies the retrograde route as a potential therapeutic target...
  16. pmc AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis
    Cedric Delevoye
    Structure and Membrane Compartments, Centre Nationale de la Recherche Scientifique, UMR 144 Institut Curie, Centre de Recherche, Paris F 75248, France
    J Cell Biol 187:247-64. 2009
    ..Our results reveal that LRO protein sorting is coupled to cell type-specific positioning of endosomes that facilitate endosome-LRO contacts and are required for organelle maturation...
  17. doi request reprint ARF6 Interacts with JIP4 to control a motor switch mechanism regulating endosome traffic in cytokinesis
    Guillaume Montagnac
    Institut Curie, Centre de Recherche, Paris, France
    Curr Biol 19:184-95. 2009
    ..ADP-ribosylation factor 6 (ARF6), which localizes to the plasma membrane and endosomal compartments, regulates endocytic recycling to the bridge during cytokinesis and is required for abscission...
  18. ncbi request reprint Exosomes: endosomal-derived vesicles shipping extracellular messages
    Benoit Fevrier
    Institut Curie, Research Section, CNRS UMR 144, 26 Rue d Ulm, 75005 Paris, France
    Curr Opin Cell Biol 16:415-21. 2004
    ..The mechanisms involved in exosome secretion and interaction with target cells are as yet unclear. A better understanding of these mechanisms is also essential to determine the link between exosomes and retroviruses...
  19. ncbi request reprint Exosomes: a bubble ride for prions?
    Benoit Fevrier
    Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, Institut Curie, 75005 Paris, France
    Traffic 6:10-7. 2005
    ..Thus, exosomes could constitute vehicles for transmission of the infectious prion protein, bypassing cell-cell contact in the dissemination of prions...
  20. pmc AP-1 and ARF1 control endosomal dynamics at sites of FcR mediated phagocytosis
    Virginie Braun
    Institut Curie, Centre de Recherche, Paris, F 75248 France
    Mol Biol Cell 18:4921-31. 2007
    ..Together, our results support a clathrin-independent role for AP complexes in endosomal dynamics in macrophages by retaining some cargo proteins, a process important for membrane remodeling during phagocytosis...
  21. doi request reprint Regulation of dendritic cell migration by CD74, the MHC class II-associated invariant chain
    Gabrielle Faure-André
    INSERM U653, Institut Curie, 12 rue Lhomond, 75005, Paris, France
    Science 322:1705-10. 2008
    ..The regulation of cell motility by Ii depended on the actin-based motor protein myosin II. Coupling antigen processing and cell motility may enable DCs to more efficiently detect and process antigens within a defined space...
  22. doi request reprint Recycling Endosome Tubule Morphogenesis from Sorting Endosomes Requires the Kinesin Motor KIF13A
    Cedric Delevoye
    Institut Curie, Centre de Recherche, Paris 75248, France Structure and Membrane Compartments, CNRS UMR144, Paris 75248, France Electronic address
    Cell Rep 6:445-54. 2014
    ..Moreover, KIF13A interacts and cooperates with RAB11 to generate endosomal tubules. Our data illustrate how a microtubule motor couples early endosome morphogenesis to its motility and function. ..
  23. pmc [Biogenesis of melanosomes - the chessboard of pigmentation]
    Cedric Delevoye
    Institut Curie, Centre de Recherche, CNRS UMR144, 26, rue d Ulm, 75248 Paris, France
    Med Sci (Paris) 27:153-62. 2011
    ..Further dissection of the melanosomal system will likely shed light not only on the biogenesis of lysosome-related organelles but also on general aspects of vesicular transport in the endosomal system...
  24. doi request reprint The ocular albinism type 1 (OA1) G-protein-coupled receptor functions with MART-1 at early stages of melanogenesis to control melanosome identity and composition
    Francesca Giordano
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Hum Mol Genet 18:4530-45. 2009
    ..These data show for the first time that melanosome composition and identity are regulated at early stages by OA1 and that MART-1 likely acts as an escort protein for this GPCR...
  25. ncbi request reprint Exosomes bearing HLA-DR1 molecules need dendritic cells to efficiently stimulate specific T cells
    Hélène Vincent-Schneider
    INSERM U520, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Int Immunol 14:713-22. 2002
    ..By contrast, the incubation of free exosomes with DC resulted in the highly efficient stimulation of specific T cells. Thus, exosomes bearing MHC class II complexes must be taken up by professional APC for efficient T cell activation...
  26. pmc Extracellular vesicles: exosomes, microvesicles, and friends
    Graca Raposo
    Institut Curie, Centre de Recherche, F 75248 Paris, Cedex 05, France
    J Cell Biol 200:373-83. 2013
    ..In this review, we focus on the characterization of EVs and on currently proposed mechanisms for their formation, targeting, and function...
  27. doi request reprint Synchronization of secretory protein traffic in populations of cells
    Gaelle Boncompain
    Institut Curie, Centre de Recherche, Paris, France
    Nat Methods 9:493-8. 2012
    ..The system should help increase the understanding of the mechanisms of trafficking and enable screens for molecules that perturb pathological protein transport...
  28. pmc ARF6 controls post-endocytic recycling through its downstream exocyst complex effector
    Magali Prigent
    Membrane and Cytoskeleton Dynamics Group, UMR144 Centre National de la Recherche Scientifique, Institut Curie, 26 Rue d Ulm, F 75248 Paris Cedex 05, France
    J Cell Biol 163:1111-21. 2003
    ..Our paper suggests that ARF6 specifies delivery and insertion of recycling membranes to regions of dynamic reorganization of the plasma membrane through interaction with the vesicle-tethering exocyst complex...
  29. ncbi request reprint Lysosome-related organelles: a view from immunity and pigmentation
    Graca Raposo
    Centre National de la Recherche Scientifique, UMR 144, Institut Curie, 75005 Paris, France
    Cell Struct Funct 27:443-56. 2002
    ..In this review we highlight adaptations and malfunction of the endosomal/lysosomal system in normal and pathological situations with special focus on MHC class II compartments in antigen presenting cells and melanosomes in pigment cells...
  30. ncbi request reprint Shiga toxin induces tubular membrane invaginations for its uptake into cells
    Winfried Römer
    Institut Curie, Centre de Recherche, Laboratoire Trafic, Signalisation et Ciblage Intracellulaires, Paris Cedex 05, France
    Nature 450:670-5. 2007
    ..The physical principles underlying this basic cargo-induced membrane uptake may also be relevant to other internalization processes, creating a rationale for conceptualizing the perplexing diversity of endocytic routes...
  31. ncbi request reprint The retromer complex and clathrin define an early endosomal retrograde exit site
    Vincent Popoff
    Laboratoire Trafic et Signalisation, UMR144 Curie CNRS, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    J Cell Sci 120:2022-31. 2007
    ..Our findings thus establish a role for the retromer complex in retrograde transport of the B-subunit of Shiga toxin, and strongly suggest that clathrin and retromer function in consecutive retrograde sorting steps on early endosomes...
  32. ncbi request reprint Myosin Ib modulates the morphology and the protein transport within multi-vesicular sorting endosomes
    Laura Salas-Cortes
    Institut Curie, CNRS UMR144, 26 Rue d Ulm, 75248, Paris, Cedex 05, France
    J Cell Sci 118:4823-32. 2005
    ..All together, these observations suggest that myosin 1b controls the traffic of protein cargo in multivesicular endosomes most probably through its ability to modulate with actin the morphology of these sorting endosomes...
  33. ncbi request reprint [Exosomes: carriers of prions?]
    Benoit Fevrier
    UMR144 CNRS, Institut Curie, 26, rue d Ulm, 75248 Paris Cedex 05, France
    Med Sci (Paris) 21:132-3. 2005
  34. pmc The ocular albinism type 1 (OA1) GPCR is ubiquitinated and its traffic requires endosomal sorting complex responsible for transport (ESCRT) function
    Francesca Giordano
    Institut Curie, Centre de Recherche, Paris F 75248, France
    Proc Natl Acad Sci U S A 108:11906-11. 2011
    ..This study highlights the role of ubiquitination and the ESCRT machinery in the intracellular trafficking of mammalian GPCRs and has implications for the physiopathology of ocular albinism type 1...
  35. pmc Melanosomes--dark organelles enlighten endosomal membrane transport
    Graca Raposo
    Institut Curie, Centre de Recherche, Paris, F 75248 France
    Nat Rev Mol Cell Biol 8:786-97. 2007
    ..Moreover, genetic disorders that affect the biogenesis of melanosomes and other lysosome-related organelles have shed light onto the molecular machinery that controls specialized endosomal sorting events...
  36. doi request reprint Isolation and characterization of exosomes from cell culture supernatants and biological fluids
    Clotilde Thery
    Institut Curie, Paris, France
    Curr Protoc Cell Biol . 2006
    ..This unit describes different approaches for exosome purification from various sources, and discusses methods to evaluate the purity and homogeneity of the purified exosome preparations...
  37. ncbi request reprint Prions and exosomes: from PrPc trafficking to PrPsc propagation
    Isabel Porto-Carreiro
    Institut Curie, CNRS UMR 144, 75248 Paris, France
    Blood Cells Mol Dis 35:143-8. 2005
    ..These findings open the possibility that methods to interfere with trafficking of such unconventional pathogens could be envisioned from insights on the mechanisms involved in exosome formation, secretion and targeting...
  38. doi request reprint Exosome secretion: molecular mechanisms and roles in immune responses
    Angélique Bobrie
    Institut Curie, Paris, France
    Traffic 12:1659-68. 2011
    ....
  39. ncbi request reprint The dark side of lysosome-related organelles: specialization of the endocytic pathway for melanosome biogenesis
    Graca Raposo
    UMR 144, Institut Curie, CNRS, Paris, Cedex 75005, France
    Traffic 3:237-48. 2002
    ..Further dissection of the melanosomal system will likely shed light not only on the biogenesis of lysosome-related organelles but also on general aspects of vesicular transport in the endosomal system...
  40. pmc ADP ribosylation factor 6 is activated and controls membrane delivery during phagocytosis in macrophages
    Florence Niedergang
    Membrane and Cytoskeleton Dynamics Laboratory, UMR144 CNRS, Institut Curie, 26 Rue d Ulm, F 75248 Paris Cedex 05, France
    J Cell Biol 161:1143-50. 2003
    ..This resulted in an early blockade in pseudopod extension and accumulation of intracellular vesicles, as observed by electron microscopy. We conclude that ARF6 is a major regulator of membrane recycling during phagocytosis...
  41. ncbi request reprint Rab6-interacting protein 1 links Rab6 and Rab11 function
    Stéphanie Miserey-Lenkei
    Institut Curie, Centre de Recherche, Paris, 75248 France
    Traffic 8:1385-403. 2007
    ..We propose that R6IP1 may couple Rab6 and Rab11 function throughout the cell cycle...
  42. pmc Identification and characterization of multiple novel Rab-myosin Va interactions
    Andrew J Lindsay
    Molecular Cell Biology Laboratory, School of Biochemistry and Cell Biology, Biosciences Institute, University College Cork, Cork, Ireland Centre de Recherche, Molecular Mechanisms of Intracellular Transport, Institut Curie, CNRS UMR144, F 75248 Paris, France School of Biochemistry and Immunology, Trinity College, Dublin 2, Ireland Structure and Membrane Compartments, Institut Curie, CNRS UMR144, F 75248 Paris, France Cell and Tissue Imaging Facility PICT IBiSA, Institut Curie, CNRS UMR144, F 75248 Paris, France
    Mol Biol Cell 24:3420-34. 2013
    ..We also present evidence that myosin Va is necessary for maintaining a peripheral distribution of Rab11- and Rab14-positive endosomes. ..
  43. doi request reprint The gene responsible for Dyggve-Melchior-Clausen syndrome encodes a novel peripheral membrane protein dynamically associated with the Golgi apparatus
    Ariane Dimitrov
    UMR CNRS 144, Institut Curie, 75005 Paris, France
    Hum Mol Genet 18:440-53. 2009
    ..These data indicate that DMC results from a loss-of-function of Dymeclin, a novel peripheral membrane protein which shuttles rapidly between the cytosol and mature Golgi membranes and point out a role of Dymeclin in cellular trafficking...
  44. pmc Sas-4 proteins are required during basal body duplication in Paramecium
    Delphine Gogendeau
    Institut Curie, Center de Recherche, Paris F 75248, France
    Mol Biol Cell 22:1035-44. 2011
    ..In addition, if daughter basal bodies were formed, they invariably had defects in MT recruitment. Our results suggest that PtSas4 has a broader function than its animal orthologues...
  45. doi request reprint Diaphanous-related formins are required for invadopodia formation and invasion of breast tumor cells
    Floria Lizarraga
    Research Center, Institut Curie, Paris, France
    Cancer Res 69:2792-800. 2009
    ....
  46. ncbi request reprint NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells
    Ariel Savina
    Institut Curie, INSERM U653, Immunité et Cancer, 26 Rue d Ulm, 75248 Paris, Cedex 05, France
    Cell 126:205-18. 2006
    ..Therefore, NOX2 plays a critical role in conferring DCs the ability to function as specialized phagocytes adapted to process antigens rather than kill pathogens...
  47. ncbi request reprint Exosomal-like vesicles are present in human blood plasma
    Marie Pierre Caby
    Institut National de la Sante et de la Recherche Medicale, Unité 520, Institut Curie, 12 rue Lhomond, 75005 Paris, France
    Int Immunol 17:879-87. 2005
    ..Our results demonstrate that blood is a physiological fluid for exosome circulation in the body, suggesting their role in cell-cell or organ-organ communications as carriers for molecules that need to reach distant cell targets...
  48. ncbi request reprint Human macrophages accumulate HIV-1 particles in MHC II compartments
    Graca Raposo
    Institut Curie, CNRS UMR 144, Paris, France
    Traffic 3:718-29. 2002
    ..Our observations also suggest direct fusion of the virus containing major histocompatibility complex class II compartment with the plasma membrane, leading to massive release of viral particles into the extracellular medium...