Genomes and Genes
Stuart A Ralph
Affiliation: Institut Pasteur
- Transcriptome analysis of antigenic variation in Plasmodium falciparum--var silencing is not dependent on antisense RNAStuart A Ralph
Institut Pasteur, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2581, F 75724 Paris Cedex 15, France
Genome Biol 6:R93. 2005..To investigate transcriptional features of this mechanism, we conducted a microarray analysis of parasites that were selected to express different var genes by adhesion to chondroitin sulfate A (CSA) or CD36...
- The epigenetic control of antigenic variation in Plasmodium falciparumStuart A Ralph
Institut Pasteur, Biology of Host Parasite Interactions, URA 2581, 25 rue du Docteur Roux, Paris, 75015, France
Curr Opin Microbiol 8:434-40. 2005..Recent findings indicate that reversible chromatin modifications and perinuclear gene movement are epigenetic factors that define the silent and active states of telomere-adjacent var genes...
- Strange organelles--Plasmodium mitochondria lack a pyruvate dehydrogenase complexStuart A Ralph
Institut Pasteur, Biology of Host Parasite Interactions Unit CNRS URA2581 25, rue du Docteur Roux, F 75724 Paris Cedex 15, France
Mol Microbiol 55:1-4. 2005..However, the absence of a corresponding mitochondrial complex suggests that energy generation in Plasmodium is considerably different from pathways described in other eukaryotes...
- Antigenic variation in Plasmodium falciparum is associated with movement of var loci between subnuclear locationsStuart A Ralph
Institut Pasteur, Centre National de la Recherche Scientifique, Unité de Recherche Associée 2581, 25 rue du Docteur Roux, F 75724 Paris Cedex 15, France
Proc Natl Acad Sci U S A 102:5414-9. 2005..Our results demonstrate that regulation of antigenic variation is associated with subnuclear position effects and point to the existence of transcriptionally permissive perinuclear zones for var genes...
- Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasitesLucio H Freitas-Junior
Institut Pasteur, Biology of Host Parasite Interactions Unit CNRS URA2581, 25, rue du Dr Roux, 75724 Paris, France
Cell 121:25-36. 2005..Our data demonstrate that mutually exclusive transcription of var genes is linked to the dynamic remodeling of chromatin...
- Differential sub-nuclear localisation of repressive and activating histone methyl modifications in P. falciparumNeha Issar
Institut Pasteur CNRS URA2581, Batiment Nicolle, Biology of Host Parasite Interactions Unit, 25 rue du Docteur Roux, F75724 Paris Cedex 15, France
Microbes Infect 11:403-7. 2009..Our data show the presence of discrete sub-nuclear zones enriched for histone methyl marks, pointing to the existence of specialized transcription factories and repressive regions in Plasmodium...
- Tropical infectious diseases: metabolic maps and functions of the Plasmodium falciparum apicoplastStuart A Ralph
Institut Pasteur, Biology of Host Parasite Interactions, 25 rue du Docteur Roux, 75724, Paris, Cedex 15, France
Nat Rev Microbiol 2:203-16. 2004
- Theileria apicoplast as a target for chemotherapyRegina Lizundia
Département Maladies Infectieuses, Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, UMR 8104 CNRS U567 INSERM, Hôpital Cochin Bâtiment Gustave Roussy, Institut Cochin, Paris, France
Antimicrob Agents Chemother 53:1213-7. 2009..Putative inhibitors of these targets were screened, and we identified antiproliferative compounds that merit further characterization...
- Genome sequence of the human malaria parasite Plasmodium falciparumMalcolm J Gardner
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:498-511. 2002..The genome sequence provides the foundation for future studies of this organism, and is being exploited in the search for new drugs and vaccines to fight malaria...
- Genome sequence of Theileria parva, a bovine pathogen that transforms lymphocytesMalcolm J Gardner
Institute for Genomic Research TIGR, 9712 Medical Center Drive, Rockville, MD 20850, USA
Science 309:134-7. 2005..Several biosynthetic pathways are incomplete or absent, suggesting substantial metabolic dependence on the host cell. One protein family that may generate parasite antigenic diversity is not telomere-associated...
- Evolution of malaria parasite plastid targeting sequencesChristopher J Tonkin
School of Botany, University of Melbourne, Melbourne, Victoria 3010, Australia
Proc Natl Acad Sci U S A 105:4781-5. 2008..Thus, facile acquisition of transit peptides from existing sequence likely expedited endosymbiont integration through intracellular gene transfer...
- Subcellular multitasking - multiple destinations and roles for the Plasmodium falcilysin proteaseStuart A Ralph
The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
Mol Microbiol 63:309-13. 2007..Plasmodium accomplishes this triple act by targeting products of the single falcilysin gene to multiple compartments...
- Membrane transporters in the relict plastid of malaria parasitesKylie A Mullin
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Victoria 3010, Australia
Proc Natl Acad Sci U S A 103:9572-7. 2006..Thus, the transporters could shunt glycolytic derivatives of glucose scavenged from the host into the plastid providing carbon, reducing equivalents and ATP to power the organelle...
- A type II pathway for fatty acid biosynthesis presents drug targets in Plasmodium falciparumRoss F Waller
School of Biochemistry and Molecular Biology, University of Melbourne, Melbourne, Victoria 3010, Australia
Antimicrob Agents Chemother 47:297-301. 2003..This study substantiates the importance of fatty acid synthesis for blood-stage parasite survival and shows that this pathway provides scope for the development of novel antimalarial drugs...
- Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparumBernardo J Foth
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, VIC 3010, Australia
Science 299:705-8. 2003..Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting...
- Phenotypic variation of Plasmodium falciparum merozoite proteins directs receptor targeting for invasion of human erythrocytesManoj T Duraisingh
The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Plant Cell Biology Research Centre, School of Botany, University of Melbourne 3010, Australia
EMBO J 22:1047-57. 2003..Phenotypic variation of the PfRh protein family allows P. falciparum to exploit different patterns of receptors on the erythrocyte surface and thereby respond to polymorphisms in erythrocyte receptors and to evade the host immune system...
- Properties and prediction of mitochondrial transit peptides from Plasmodium falciparumAndreas Bender
Johann Wolfgang Goethe Universitat Frankfurt, Institut für Organische Chemie und Chemische Biologie, Marie Curie Strasse 11, D 60439, Frankfurt, Germany
Mol Biochem Parasitol 132:59-66. 2003..51 (84% correct prediction) in a 10-fold cross-validation study. It predicted 381 (7.1%) mitochondrial genes, based on 5334 annotated genes in the P. falciparum genome...
- Evolutionary pressures on apicoplast transit peptidesStuart A Ralph
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia
Mol Biol Evol 21:2183-94. 2004..These unusual features are explained in the context of secondary plastid trafficking via the endomembrane system...