Pascal Pineau

Summary

Affiliation: Institut Pasteur
Location: Paris, France
Summary:
Molecular Epidemiology of primary liver cancer
(mutations, oncogenes, tumor suppressors, chromosomes loss, CGH, methylation, expression changes, microRNA, viral variants)

Publications

  1. pmc p53 regulates epithelial-mesenchymal transition through microRNAs targeting ZEB1 and ZEB2
    Taewan Kim
    Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, 2 Molecular, Cellular, and Developmental Biology Program, The Ohio State University, Columbus, OH 43210, USA
    J Exp Med 208:875-83. 2011
  2. ncbi request reprint [A review of cellular senescence: friend or foe of tumorigenesis?]
    Oliver Bischof
    Unité d Organisation Nucléaire et Oncogenèse ONO, INSERM U579, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Med Sci (Paris) 25:153-60. 2009
  3. ncbi request reprint MDM2 SNP309T>G polymorphism and risk of hepatocellular carcinoma: a case-control analysis in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morocco
    Cancer Detect Prev 32:380-5. 2009
  4. pmc The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer
    Agustin F Fernandez
    Cancer Epigenetics Group, Spanish National Cancer Research Centre CNIO, Madrid E 28029, Spain
    Genome Res 19:438-51. 2009
  5. ncbi request reprint Chromosome instability in human hepatocellular carcinoma depends on p53 status and aflatoxin exposure
    Pascal Pineau
    Unité d Organisation Nucléaire et Oncogénèse, INSERM U579, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mutat Res 653:6-13. 2008
  6. doi request reprint Genotype determination in Moroccan hepatitis B chronic carriers
    Sayeh Ezzikouri
    Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100 Casablanca, Maroc, Morocco
    Infect Genet Evol 8:306-12. 2008
  7. doi request reprint Prevalence of common HFE and SERPINA1 mutations in patients with hepatocellular carcinoma in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morocco
    Arch Med Res 39:236-41. 2008
  8. ncbi request reprint Genetic polymorphism in the manganese superoxide dismutase gene is associated with an increased risk for hepatocellular carcinoma in HCV-infected Moroccan patients
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100, Casablanca, Morocco
    Mutat Res 649:1-6. 2008
  9. ncbi request reprint The Pro variant of the p53 codon 72 polymorphism is associated with hepatocellular carcinoma in Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morrocco
    Hepatol Res 37:748-54. 2007
  10. ncbi request reprint Single nucleotide polymorphism in DNMT3B promoter and its association with hepatocellular carcinoma in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire des hépatites virales, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100 Casablanca, Morocco
    Infect Genet Evol 9:877-81. 2009

Detail Information

Publications41

  1. pmc p53 regulates epithelial-mesenchymal transition through microRNAs targeting ZEB1 and ZEB2
    Taewan Kim
    Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, 2 Molecular, Cellular, and Developmental Biology Program, The Ohio State University, Columbus, OH 43210, USA
    J Exp Med 208:875-83. 2011
    ..Inhibition or overexpression of the miRNAs affects p53-regulated EMT by altering ZEB1 and ZEB2 expression. Our findings indicate that p53 can regulate EMT, and that p53-regulated miRNAs are critical mediators of p53-regulated EMT...
  2. ncbi request reprint [A review of cellular senescence: friend or foe of tumorigenesis?]
    Oliver Bischof
    Unité d Organisation Nucléaire et Oncogenèse ONO, INSERM U579, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Med Sci (Paris) 25:153-60. 2009
    ..Here, we take a snapshot of the progress in understanding the causes and consequences of cellular senescence in vitro and in vivo...
  3. ncbi request reprint MDM2 SNP309T>G polymorphism and risk of hepatocellular carcinoma: a case-control analysis in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morocco
    Cancer Detect Prev 32:380-5. 2009
    ..The aim of this study was to investigate whether this functional SNP is associated with an enhanced risk of liver tumorigenesis in Moroccan patients...
  4. pmc The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer
    Agustin F Fernandez
    Cancer Epigenetics Group, Spanish National Cancer Research Centre CNIO, Madrid E 28029, Spain
    Genome Res 19:438-51. 2009
    ..The observed DNA methylation changes have a major functional impact on the biological behavior of the viruses...
  5. ncbi request reprint Chromosome instability in human hepatocellular carcinoma depends on p53 status and aflatoxin exposure
    Pascal Pineau
    Unité d Organisation Nucléaire et Oncogénèse, INSERM U579, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mutat Res 653:6-13. 2008
    ..4% vs. 16.7%, p<0.0001). We suggest that AFB1-associated mutagenesis represents a plausible cause for the higher chromosome instability observed in Chinese HCCs, when compared with European primary liver carcinomas...
  6. doi request reprint Genotype determination in Moroccan hepatitis B chronic carriers
    Sayeh Ezzikouri
    Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100 Casablanca, Maroc, Morocco
    Infect Genet Evol 8:306-12. 2008
    ..Treatment of chronic hepatitis B is often complicated by the appearance of escape mutants after treatment with nucleoside analogs, especially with genotypes responsible for the more severe form of the disease...
  7. doi request reprint Prevalence of common HFE and SERPINA1 mutations in patients with hepatocellular carcinoma in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morocco
    Arch Med Res 39:236-41. 2008
    ..Our objective was to estimate the prevalence of HFE and SERPINA1 (formerly known as alpha1-antitrypsin, AAT) mutations and assess their influence on hepatocellular carcinoma development...
  8. ncbi request reprint Genetic polymorphism in the manganese superoxide dismutase gene is associated with an increased risk for hepatocellular carcinoma in HCV-infected Moroccan patients
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100, Casablanca, Morocco
    Mutat Res 649:1-6. 2008
    ..8% in the control subjects OR, 5.09; 95% CI, 1.76-14.66). Our findings provide further evidence of an association between the Ala-9Val MnSOD polymorphism and HCC occurrence in hepatitis C virus-infected Moroccan patients...
  9. ncbi request reprint The Pro variant of the p53 codon 72 polymorphism is associated with hepatocellular carcinoma in Moroccan population
    Sayeh Ezzikouri
    Laboratoire de Virologie, Institut Pasteur du Maroc, Casablanca, Morrocco
    Hepatol Res 37:748-54. 2007
    ..31-fold increased risk for HCC. Conclusion: These results provide evidence that p53 polymorphism at codon 72 is a modifier of hepatocarcinogenesis, especially in women and HCV-negative subjects...
  10. ncbi request reprint Single nucleotide polymorphism in DNMT3B promoter and its association with hepatocellular carcinoma in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire des hépatites virales, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20100 Casablanca, Morocco
    Infect Genet Evol 9:877-81. 2009
    ..In addition, we provide genetic evidence for the major difference of HCC risk between men and women. Further mechanistic studies are needed to unravel the underlying molecular mechanisms...
  11. pmc Mutational analysis of TP53, PTEN, PIK3CA and CTNNB1/beta-catenin genes in human herpesvirus 8-associated primary effusion lymphoma
    Emmanuelle Boulanger
    Department of Clinical Immunology, Hopital Saint Louis, Assistance Publique Hopitaux de Paris, Paris, France
    Haematologica 94:1170-4. 2009
    ....
  12. pmc Somatic hypermutation of human mitochondrial and nuclear DNA by APOBEC3 cytidine deaminases, a pathway for DNA catabolism
    Rodolphe Suspène
    Institut Pasteur, Molecular Retrovirology Unit, Centre National de la Recherche Scientifique URA3015, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 108:4858-63. 2011
    ..Nonetheless, hyper- and lightly mutated sequences went hand in hand, raising the hypothesis that recurrent low-level mutation by APOBEC3A could catalyze the transition from a healthy to a cancer genome...
  13. pmc First multicenter study for risk factors for hepatocellular carcinoma development in North Africa
    Olfa Bahri
    Olfa Bahri, Hinda Triki, Laboratory of Clinical Virology, Institut Pasteur of Tunis, Tunis, BP 1002, Tunisia
    World J Hepatol 3:24-30. 2011
    ..To assess the role of the major risk factors for hepatocellular carcinoma (HCC) development in the western part of North Africa...
  14. doi request reprint Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC): molecular mechanisms and novel paradigms
    C Brechot
    INSERM U785, Liver Hepatology centre, Paul Brousse Hospital, University Paris XI, 12, avenue Paul Vaillant Couturier, 94804 Villejuif cedex 15, France
    Pathol Biol (Paris) 58:278-87. 2010
    ....
  15. pmc Massive APOBEC3 editing of hepatitis B viral DNA in cirrhosis
    Jean Pierre Vartanian
    Molecular Retrovirology Unit, Institut Pasteur, Paris, France
    PLoS Pathog 6:e1000928. 2010
    ..Although the mutant spectrum resulting from A3 editing is highly deleterious, a very small part, notably the lightly edited genomes, might help the virus evolve and even escape immune responses...
  16. pmc Reprogramming of miRNA networks in cancer and leukemia
    Stefano Volinia
    Data Mining for Analysis of Microarrays, Department of Morphology and Embryology, Universita degli Studi, Ferrara 44100, Italy
    Genome Res 20:589-99. 2010
    ..Finally, we experimentally validated the miRNA network with acute lymphocytic leukemia originated in Mir155 transgenic mice. Most of miRNAs deregulated in these transgenic mice were located close to hsa-miR-155 in the cancer network...
  17. pmc miR-221 overexpression contributes to liver tumorigenesis
    Pascal Pineau
    Nuclear Organization and Oncogenesis Unit, Institut National de Santé et de Recherche Médicale U579, Institut Pasteur, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 107:264-9. 2010
    ..Thus, the use of synthetic inhibitors of miR-221 may prove to be a promising approach to liver cancer treatment...
  18. doi request reprint Polymorphisms in antioxidant defence genes and susceptibility to hepatocellular carcinoma in a Moroccan population
    Sayeh Ezzikouri
    Laboratoire des hépatites virales, Institut Pasteur du Maroc, 20360 Casablanca, Morocco
    Free Radic Res 44:208-16. 2010
    ..Furthermore, it is possible that cumulative defects in protection from oxidative stress may result in increased risk of liver cancer in the Moroccan population...
  19. doi request reprint [Hepatitis B vaccination: a major player in the control of primary liver cancer]
    P Pineau
    Inserm U579 Organisation nucléaire et oncogenèse, Institut Pasteur, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Pathol Biol (Paris) 58:444-53. 2010
    ..Finally, lack of early immunization of newborns in developing countries still represents a major limitation to the progresses against liver cancer...
  20. ncbi request reprint Genomic stability prevails in North-African hepatocellular carcinomas
    P Pineau
    Nuclear Organization and Oncogenesis Unit, INSERM U579, Pasteur Institute, Paris, France
    Dig Liver Dis 39:671-7. 2007
    ....
  21. ncbi request reprint Tbx3 is a downstream target of the Wnt/beta-catenin pathway and a critical mediator of beta-catenin survival functions in liver cancer
    Claire Angélique Renard
    Institut National de la Sante et de la Recherche Medicale U579, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, France
    Cancer Res 67:901-10. 2007
    ..These results reveal an unsuspected role of Tbx3 as a mediator of beta-catenin activities on cell proliferation and survival and as an important player in liver tumorigenesis...
  22. ncbi request reprint Effect of TT virus infection on hepatocellular carcinoma development: results of a Euro-Asian survey
    P Pineau
    Unite de Recombinaison et Expression Genetique, INSERM U163, Departement des Retrovirus, Institut Pasteur, Paris, France
    J Infect Dis 181:1138-42. 2000
    ..5% of the tumors. Taken together, these data suggest that TTV infection is unlikely to be associated with the induction or acceleration of the hepatocarcinogenic process in humans...
  23. ncbi request reprint Close correlation between beta-catenin gene alterations and nuclear accumulation of the protein in human hepatocellular carcinomas
    B Terris
    Service d Anatomie Pathologique, Hopital Beaujon, Clichy, France
    Oncogene 18:6583-8. 1999
    ..The close association between increased beta-catenin protein stability and mutation indicates that immunohistochemistry may be a powerful method for the detection of the mutated protein in future clinical practice...
  24. ncbi request reprint Identification of three distinct regions of allelic deletions on the short arm of chromosome 8 in hepatocellular carcinoma
    P Pineau
    Unité de Recombinaison and Expression Génétique, INSERM U163, Institut Pasteur, Paris, France
    Oncogene 18:3127-34. 1999
    ..These data strongly suggest the presence of at least three novel tumor suppressor loci on 8p in hepatocellular carcinoma...
  25. ncbi request reprint [Hepatitis B virus and hepatocellular carcinoma]
    P Tiollais
    Unité de Recombinaison et Expression Génétique INSERM U 163, Institut Pasteur, Paris
    Bull Acad Natl Med 182:73-7; discussion 77-8. 1998
    ..Study of transgenic mice have shown the liver specificity of the WHV action. In man, the liver oncogenesis is not explained. Studies are in progress to detect inactivation of tumor suppressor genes...
  26. ncbi request reprint Extensive analysis of duplicated-inverted hepatitis B virus integrations in human hepatocellular carcinoma
    P Pineau
    Unite de Recombinaison et Expression Genetique, INSERM U163, Institut Pasteur, Paris, France
    J Gen Virol 79:591-600. 1998
    ..The implications of our findings for the understanding of primary liver cancer development are discussed...
  27. ncbi request reprint Comprehensive allelotyping of human hepatocellular carcinoma
    H Nagai
    Unite de Recombinaison et Expression Genetique, INSERM U163, Institut Pasteur, Paris, France
    Oncogene 14:2927-33. 1997
    ..This first report of an extensive allelotypic analysis of HCC should help in identifying new genes whose loss of function contributes to the development of liver cancer...
  28. ncbi request reprint Recurrent chromosomal abnormalities in hepatocellular carcinoma detected by comparative genomic hybridization
    A Marchio
    Unite de Recombinaison et Expression Genetique, INSERM U163, Institut Pasteur, Paris, France
    Genes Chromosomes Cancer 18:59-65. 1997
    ..These findings should lead to the characterization of new loci involved in liver cancer pathogenesis...
  29. pmc A t(3;8) chromosomal translocation associated with hepatitis B virus intergration involves the carboxypeptidase N locus
    P Pineau
    Unite de Recombinaison et Expression Genetique, Institut National dela Santé et de la Recherche Médicale INSERM U163, Institut Pasteur, Paris, France
    J Virol 70:7280-4. 1996
    ..The present data strongly support a model in which HBV-induced chromosomal rearrangements play a key role during multistep liver oncogenesis...
  30. ncbi request reprint Studies of genetic defects in hepatocellular carcinoma: recent outcomes and new challenges
    P Pineau
    J Hepatol 33:152-6. 2000
  31. ncbi request reprint Hepatocellular carcinoma occurring in nonfibrotic liver: epidemiologic and histopathologic analysis of 80 French cases
    M P Bralet
    Départements, AP HP, Hopital Beaujon, Clichy Cedex, France
    Hepatology 32:200-4. 2000
    ..Etiologic factors are often unidentified, although presence of HBV infection in 21% suggests a direct oncogenic role of this virus...
  32. ncbi request reprint Distinct chromosomal abnormality pattern in primary liver cancer of non-B, non-C patients
    A Marchio
    Unite de Recombinaison et Expression Genetique, INSERM U163, Institut Pasteur, Paris, France
    Oncogene 19:3733-8. 2000
    ..This region, which is also preferentially amplified in non fibrolamellar tumors (58%), may contain an essential proto-oncogene commonly implicated in liver carcinogenesis...
  33. ncbi request reprint Structural organization and expression of human MTUS1, a candidate 8p22 tumor suppressor gene encoding a family of angiotensin II AT2 receptor-interacting proteins, ATIP
    M Di Benedetto
    Institut Cochin, Departement de Biologie Cellulaire, Paris, F 75014, France
    Gene 380:127-36. 2006
    ..High evolutionary conservation of ATIP amino-acid sequences suggests important biological roles for this new family of proteins in tumor suppression and/or brain function...
  34. ncbi request reprint Mutation analysis of the 8p22 candidate tumor suppressor gene ATIP/MTUS1 in hepatocellular carcinoma
    M Di Benedetto
    Institut Cochin, Departement de Biologie Cellulaire, Paris, F 75014 France
    Mol Cell Endocrinol 252:207-15. 2006
    ....
  35. ncbi request reprint A universal primer set for PCR amplification of nuclear histone H4 genes from all animal species
    Pascal Pineau
    Unité d Organisation Nucléaire et Oncogénèse, INSERM U579, Institut Pasteur, Paris, France
    Mol Biol Evol 22:582-8. 2005
    ..The data indicate that the HIST2H4 amplimers are, thus, suitable for both DNA quality testing as well as species identification in the animal kingdom...
  36. ncbi request reprint Homozygous deletions scanning in tumor cell lines detects previously unsuspected loci
    Pascal Pineau
    Unite de Recombinaison et Expression Genetique, INSERM, Département de Médecine Moléculaire, Institut Pasteur, Paris, France
    Int J Cancer 106:216-23. 2003
    ..Our data suggest that, beside allelotyping or transcriptome/proteome studies, extensive HD profiling represents a promising approach for the detection of hitherto not implicated signalling pathways of tumorigenesis...
  37. ncbi request reprint Homozygous deletion scanning in hepatobiliary tumor cell lines reveals alternative pathways for liver carcinogenesis
    Pascal Pineau
    Unite de Recombinaison et Expression Genetique, INSERM U163, Département de Médecine Moléculaire, Institut Pasteur, Paris, France
    Hepatology 37:852-61. 2003
    ..In conclusion, our data suggest that these genes may represent novel liver tumor suppressive targets. Additional tumorigenic pathways should be carefully considered in hepatocarcinogenesis...
  38. ncbi request reprint Mutation analysis of novel human liver-related putative tumor suppressor gene in hepatocellular carcinoma
    Cheng Liao
    State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China
    World J Gastroenterol 9:89-93. 2003
    ..To find the point mutations meaningful for inactivation of liver-related putative tumor suppressor gene (LPTS) gene, a human novel liver-related putative tumor suppressor gene and telomerase inhibitor in hepatocellular carcinoma...
  39. pmc Identification of a hepatitis B virus genome in wild chimpanzees (Pan troglodytes schweinfurthi) from East Africa indicates a wide geographical dispersion among equatorial African primates
    Jean Pierre Vartanian
    Unite de Retrovirologie Moleculaire, Institut Pasteur, Paris, France
    J Virol 76:11155-8. 2002
    ..An entire hepatitis B virus (HBV) genome was amplified and sequenced from samples taken from one animal. This indicates that HBV is distributed across the entire range of chimpanzee habitats...
  40. pmc Chromosomal abnormalities in liver cell dysplasia detected by comparative genomic hybridisation
    A Marchio
    Unite de Recombinaison et Expression Genetique, INSERM U163, Institut Pasteur, 75724 Paris, France
    Mol Pathol 54:270-4. 2001
    ..The aim of this study was to determine whether there is a genetic link between liver cell dysplasia and HCC that could support the role of dysplasia as a tumour precursor lesion...
  41. doi request reprint Somatic changes in primary liver cancer in Russia: a pilot study
    Olga Kalinina
    Laboratory of Molecular Microbiology, Saint Petersburg Pasteur Institute, Saint Petersburg, Russian Federation, Laboratory of Molecular Biology, Almazov Centre for Heart, Blood and Endocrinology, Saint Petersburg, Russian Federation
    Mutat Res 755:90-9. 2013
    ..An extension of the present cohort as well as further molecular studies are now warranted in order to understand the processes governing liver carcinogenesis affecting Russian populations. ..