Mathieu Picardeau

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. pmc Serovar diversity of pathogenic Leptospira circulating in the French West Indies
    Pascale Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France
    PLoS Negl Trop Dis 7:e2114. 2013
  2. pmc Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean)
    Pascale Bourhy
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Negl Trop Dis 4:e724. 2010
  3. ncbi request reprint Diagnosis and epidemiology of leptospirosis
    M Picardeau
    Institut Pasteur, Unité de Biologie des Spirochètes, centre national de référence et centre collaborateur de l OMS de la leptospirose, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Med Mal Infect 43:1-9. 2013
  4. pmc Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features
    P Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Centre for Leptospirosis, WHO Collaborating Center for the Epidemiology of Leptospirosis, Paris, France
    J Clin Microbiol 50:307-11. 2012
  5. doi request reprint Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Genetica 138:551-8. 2010
  6. pmc A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 75:677-83. 2007
  7. pmc Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3255-8. 2005
  8. ncbi request reprint Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    FEMS Microbiol Lett 225:257-62. 2003
  9. pmc Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis
    Hélène Louvel
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3249-54. 2005
  10. pmc The OmpA-like protein Loa22 is essential for leptospiral virulence
    Paula Ristow
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Pathog 3:e97. 2007

Collaborators

Detail Information

Publications34

  1. pmc Serovar diversity of pathogenic Leptospira circulating in the French West Indies
    Pascale Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France
    PLoS Negl Trop Dis 7:e2114. 2013
    ..In this study, we describe the serological and molecular features of leptospires isolated from 104 leptospirosis patients in Guadeloupe (n = 85) and Martinique (n = 19) and six rats captured in Guadeloupe, between 2004 and 2012...
  2. pmc Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean)
    Pascale Bourhy
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Negl Trop Dis 4:e724. 2010
    ..To date, Leptospira isolates have never been isolated in this endemic region...
  3. ncbi request reprint Diagnosis and epidemiology of leptospirosis
    M Picardeau
    Institut Pasteur, Unité de Biologie des Spirochètes, centre national de référence et centre collaborateur de l OMS de la leptospirose, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Med Mal Infect 43:1-9. 2013
    ..Leptospirosis is expected to become more important due to a rapid urbanization in developing countries (slums), global warming, and extreme climatic events (floods)...
  4. pmc Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features
    P Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Centre for Leptospirosis, WHO Collaborating Center for the Epidemiology of Leptospirosis, Paris, France
    J Clin Microbiol 50:307-11. 2012
    ..These results are essential for the improvement of current diagnostic tools and provide a starting point for a better understanding of the epidemiology of leptospirosis in this area of endemicity...
  5. doi request reprint Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Genetica 138:551-8. 2010
    ..Transposon-based approaches greatly facilitate studies of bacterial biology. We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria...
  6. pmc A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 75:677-83. 2007
    ..interrogans virulent strains. This genomic island, containing a high proportion of novel genes, may have an important role in spreading genes, including virulence factors, among bacterial populations...
  7. pmc Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3255-8. 2005
    ..interrogans mutants. This approach of transposon mutagenesis will be useful for understanding the spirochetal physiology and the pathogenic mechanisms of Leptospira, which remain largely unknown...
  8. ncbi request reprint Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    FEMS Microbiol Lett 225:257-62. 2003
    ..In addition, a L. interrogans gene, called metZ, was found to complement an Escherichia coli metB mutant, further suggesting that the transsulfuration pathway is also present in Leptospira spp...
  9. pmc Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis
    Hélène Louvel
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3249-54. 2005
    ..These results confirm the importance of iron for the growth of Leptospira and its ability to use multiple iron sources...
  10. pmc The OmpA-like protein Loa22 is essential for leptospiral virulence
    Paula Ristow
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Pathog 3:e97. 2007
    ..Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species...
  11. ncbi request reprint A putative regulatory genetic locus modulates virulence in the pathogen Leptospira interrogans
    Azad Eshghi
    Institut Pasteur, Biology of Spirochetes Unit, Paris, France
    Infect Immun 82:2542-52. 2014
    ..We provide insight into a possible requirement for transcriptional regulation as it relates to leptospiral virulence and suggest various biological processes that are affected due to the loss of native expression of this genetic locus. ..
  12. pmc Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences
    Pascale Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, France
    J Clin Microbiol 49:2154-60. 2011
    ..However, it is important to continually evaluate and, if necessary, modify the primers and/or probes used to ensure effective detection of the circulating Leptospira isolates...
  13. ncbi request reprint Genetic manipulation of Leptospira biflexa
    Hélène Louvel
    Unité de Biologie des Spirochètes Institut Pasteur, Paris, France
    Curr Protoc Microbiol . 2007
    ..biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes...
  14. doi request reprint Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis
    Kristel Lourdault
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    J Med Microbiol 58:648-55. 2009
    ..qPCR will be used in future studies to evaluate the efficacy of vaccine candidates against leptospirosis and the virulence of selected L. interrogans mutants relative to the parental strain...
  15. pmc Inactivation of clpB in the pathogen Leptospira interrogans reduces virulence and resistance to stress conditions
    Kristel Lourdault
    Unité de Biologie des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 79:3711-7. 2011
    ..Our findings demonstrate that ClpB is involved in the general stress response. The chaperone is also necessary, either directly or indirectly, for the virulence of the pathogen L. interrogans...
  16. pmc The scc spirochetal coiled-coil protein forms helix-like filaments and binds to nucleic acids generating nucleoprotein structures
    Khalil Mazouni
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 188:469-76. 2006
    ..These results provide the first insight into a unique DNA binding filament-forming coiled-coil protein that could play an important role in the subcellular architecture of the spirochetal microorganism...
  17. ncbi request reprint Leptospira spp. possess a complete haem biosynthetic pathway and are able to use exogenous haem sources
    Rozenn Guégan
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    Mol Microbiol 49:745-54. 2003
    ..This may have implications in a better understanding of the pathogenesis of Leptospira...
  18. doi request reprint Evaluation of an in-house ELISA using the intermediate species Leptospira fainei for diagnosis of leptospirosis
    Pascale Bourhy
    Institut Pasteur, Biology of Spirochetes Unit, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France
    J Med Microbiol 62:822-7. 2013
    ..The overall performance of this in-house ELISA suggests applicability as a rapid screening test for the diagnosis of leptospirosis in resource-limited settings and in hospitals and laboratories where a MAT is not available...
  19. doi request reprint Construction of a library of random mutants in the spirochete Leptospira biflexa using a mariner transposon
    Leyla Slamti
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris Cedex 15, France
    Methods Mol Biol 859:169-76. 2012
    ..This chapter describes a procedure for transposition in L. biflexa via conjugation of a plasmid delivering Himar1, isolation of mutants, and mapping of the insertion sites on the chromosome...
  20. pmc FlaA proteins in Leptospira interrogans are essential for motility and virulence but are not required for formation of the flagellum sheath
    Ambroise Lambert
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    Infect Immun 80:2019-25. 2012
    ..This study shows that FlaA subunits contribute to leptospiral translational motility, cellular shape, and virulence...
  21. pmc Heme rescues a two-component system Leptospira biflexa mutant
    Hélène Louvel
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    BMC Microbiol 8:25. 2008
    ..Heme is typically a major iron source for bacteria, but little is known about how bacteria of the Leptospira genus, composed of both saprophytic and pathogenic species, access heme...
  22. pmc Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin
    Claudio Pereira Figueira
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris Cedex 15, France
    BMC Microbiol 11:129. 2011
    ..Here, we describe a system for heterologous expression of the Leptospira interrogans genes ligA and ligB in the saprophyte Leptospira biflexa serovar Patoc...
  23. pmc Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    Appl Environ Microbiol 74:319-22. 2008
    ..interrogans, respectively. Analysis of mutants indicated that transposition occurs randomly, and at single sites in the genome of these strains, allowing the utilization of this system to generate libraries of transposon mutants...
  24. ncbi request reprint The spirochetal chpK-chromosomal toxin-antitoxin locus induces growth inhibition of yeast and mycobacteria
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris15, France
    FEMS Microbiol Lett 229:277-81. 2003
    ..These results show that ChpK possesses a broad activity against both prokaryotes and eukaryotes, suggesting that the cellular target of the toxin is conserved in these organisms...
  25. pmc Expanding the genetic toolbox for Leptospira species by generation of fluorescent bacteria
    Florence Aviat
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    Appl Environ Microbiol 76:8135-42. 2010
    ..Finally, we showed that GFP can be used as a reporter to assess promoter activity in different environmental conditions. These results may facilitate further advances for studying the genetics of Leptospira spp...
  26. doi request reprint Biofilm formation by saprophytic and pathogenic leptospires
    Paula Ristow
    Unité de Biologie des Spirochètes, Institut Pasteur, 75724 Paris Cedex 15, France
    Microbiology 154:1309-17. 2008
    ..The formation of such a biofilm is consistent with the life of saprophytic strains in water and may help pathogenic strains to survive in environmental habitats and to colonize the host...
  27. pmc Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis
    Laurence Salaun
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 44:3954-62. 2006
    ..Our results show that this simple PCR-based MLVA typing technique is a powerful methodology for the epidemiology of leptospirosis...
  28. pmc Deciphering morphological determinants of the helix-shaped Leptospira
    Leyla Slamti
    Institut Pasteur, Unité Biologie des Spirochètes, 25 rue du Dr Roux, 75015 Paris, France
    J Bacteriol 193:6266-75. 2011
    ....
  29. pmc Complete nucleotide sequence of the LE1 prophage from the spirochete Leptospira biflexa and characterization of its replication and partition functions
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3931-40. 2005
    ..These results enable the construction of new shuttle vectors for studying the genetics of Leptospira spp. This study will also contribute to a better knowledge of phages unrelated to lambdoid phages...
  30. pmc Antibiotic resistance markers for genetic manipulations of Leptospira spp
    Dante Poggi
    Unité de Biologie des Spirochètes, Institut Pasteur, 75724 Paris Cedex 15, France
    Appl Environ Microbiol 76:4882-5. 2010
    ..We also generated a gentamicin resistance cassette that allows the use of a third selectable marker in leptospires. These results may facilitate further advances in gene transfer systems in Leptospira spp...
  31. pmc Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology
    Ange Patricia Tchamedeu Kameni
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    J Bacteriol 184:452-8. 2002
    ..e., DNA is condensed at the midcell. Our data indicate that L. biflexa RecA plays a major role in ensuring cell viability via mechanisms such as DNA repair and, indirectly, active chromosome partitioning...
  32. pmc Chemotactic behavior of pathogenic and nonpathogenic Leptospira species
    Ambroise Lambert
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    Appl Environ Microbiol 78:8467-9. 2012
    ..This assay has the general applicability to further our understanding of leptospiral chemotaxis...
  33. pmc Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS ONE 3:e1607. 2008
    ..borgpetersenii has impaired its survival outside a mammalian host, whereas L. interrogans has retained environmental sensory functions that facilitate disease transmission through water...
  34. ncbi request reprint Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri
    Hélène Bauby
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 149:689-93. 2003
    ..The authors have also characterized a second selectable marker that allows the construction of a spectinomycin-resistant L. meyeri-E. coli shuttle vector and the functional complementation of the L. meyeri trpE mutant...