M Picardeau

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. ncbi request reprint Analysis of the internal replication region of a mycobacterial linear plasmid
    M Picardeau
    Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France
    Microbiology 146:305-13. 2000
  2. pmc A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 75:677-83. 2007
  3. pmc Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa
    Philip E Stewart
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St, Hamilton, Montana 59840, USA
    BMC Microbiol 12:290. 2012
  4. pmc Heme rescues a two-component system Leptospira biflexa mutant
    Hélène Louvel
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    BMC Microbiol 8:25. 2008
  5. pmc Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    Appl Environ Microbiol 74:319-22. 2008
  6. pmc Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin
    Claudio Pereira Figueira
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris Cedex 15, France
    BMC Microbiol 11:129. 2011
  7. ncbi request reprint The spirochetal chpK-chromosomal toxin-antitoxin locus induces growth inhibition of yeast and mycobacteria
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris15, France
    FEMS Microbiol Lett 229:277-81. 2003
  8. ncbi request reprint Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    FEMS Microbiol Lett 225:257-62. 2003
  9. pmc Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli
    M Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    J Bacteriol 183:6494-7. 2001
  10. ncbi request reprint First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella
    M Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 40:189-99. 2001

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Analysis of the internal replication region of a mycobacterial linear plasmid
    M Picardeau
    Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France
    Microbiology 146:305-13. 2000
    ..Thus, this new Escherichia coli-mycobacteria shuttle vector may be used in both slow- and fast-growing mycobacteria and in co-transformation experiments with other mycobacterial vectors...
  2. pmc A genomic island of the pathogen Leptospira interrogans serovar Lai can excise from its chromosome
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 75:677-83. 2007
    ..interrogans virulent strains. This genomic island, containing a high proportion of novel genes, may have an important role in spreading genes, including virulence factors, among bacterial populations...
  3. pmc Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa
    Philip E Stewart
    Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St, Hamilton, Montana 59840, USA
    BMC Microbiol 12:290. 2012
    ..Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific function for these proteins has not been experimentally demonstrated...
  4. pmc Heme rescues a two-component system Leptospira biflexa mutant
    Hélène Louvel
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    BMC Microbiol 8:25. 2008
    ..Heme is typically a major iron source for bacteria, but little is known about how bacteria of the Leptospira genus, composed of both saprophytic and pathogenic species, access heme...
  5. pmc Conjugative transfer between Escherichia coli and Leptospira spp. as a new genetic tool
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    Appl Environ Microbiol 74:319-22. 2008
    ..interrogans, respectively. Analysis of mutants indicated that transposition occurs randomly, and at single sites in the genome of these strains, allowing the utilization of this system to generate libraries of transposon mutants...
  6. pmc Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin
    Claudio Pereira Figueira
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris Cedex 15, France
    BMC Microbiol 11:129. 2011
    ..Here, we describe a system for heterologous expression of the Leptospira interrogans genes ligA and ligB in the saprophyte Leptospira biflexa serovar Patoc...
  7. ncbi request reprint The spirochetal chpK-chromosomal toxin-antitoxin locus induces growth inhibition of yeast and mycobacteria
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris15, France
    FEMS Microbiol Lett 229:277-81. 2003
    ..These results show that ChpK possesses a broad activity against both prokaryotes and eukaryotes, suggesting that the cellular target of the toxin is conserved in these organisms...
  8. ncbi request reprint Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp
    Mathieu Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    FEMS Microbiol Lett 225:257-62. 2003
    ..In addition, a L. interrogans gene, called metZ, was found to complement an Escherichia coli metB mutant, further suggesting that the transsulfuration pathway is also present in Leptospira spp...
  9. pmc Killing effect and antitoxic activity of the Leptospira interrogans toxin-antitoxin system in Escherichia coli
    M Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    J Bacteriol 183:6494-7. 2001
    ..The coexpression of ChpI neutralizes ChpK toxicity. By Southern blot analysis, chp homologs were found in all representative pathogenic strains of L. interrogans...
  10. ncbi request reprint First evidence for gene replacement in Leptospira spp. Inactivation of L. biflexa flaB results in non-motile mutants deficient in endoflagella
    M Picardeau
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 40:189-99. 2001
    ..Our results demonstrate that FlaB is involved in flagella assembly and motility. They also show the feasibility of performing allelic replacement in Leptospira spp. by homologous recombination...
  11. pmc Genomic sequence and transcriptional analysis of a 23-kilobase mycobacterial linear plasmid: evidence for horizontal transfer and identification of plasmid maintenance systems
    C Le Dantec
    Laboratoire de Référence des Mycobactéries, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 183:2157-64. 2001
    ..Functional analysis of these ORFs suggests that they probably carry out similar maintenance roles in pCLP...
  12. ncbi request reprint Mycobacterial linear plasmids have an invertron-like structure related to other linear replicons in actinomycetes
    M Picardeau
    Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France
    Microbiology 144:1981-8. 1998
    ....
  13. ncbi request reprint Leptospira spp. possess a complete haem biosynthetic pathway and are able to use exogenous haem sources
    Rozenn Guégan
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    Mol Microbiol 49:745-54. 2003
    ..This may have implications in a better understanding of the pathogenesis of Leptospira...
  14. pmc The LE1 bacteriophage replicates as a plasmid within Leptospira biflexa: construction of an L. biflexa-Escherichia coli shuttle vector
    I S Girons
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 182:5700-5. 2000
    ..Our data indicate that the replicon derived from the LE1 leptophage, together with the kanamycin resistance gene, is a promising tool with which to develop the genetics of Leptospira species...
  15. ncbi request reprint Erp, an extracellular protein family specific to mycobacteria
    L de Mendonça-Lima
    Unite de Genetique Mycobacterienne, Institut Pasteur, F 75724 Paris Cedex 15, France
    Microbiology 147:2315-20. 2001
    ..The genome sequence flanking the erp gene includes cell-wall-related ORFs and displays extensive conservation between saprophytic and pathogenic mycobacteria...
  16. pmc Isolation and characterization of FecA- and FeoB-mediated iron acquisition systems of the spirochete Leptospira biflexa by random insertional mutagenesis
    Hélène Louvel
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3249-54. 2005
    ..These results confirm the importance of iron for the growth of Leptospira and its ability to use multiple iron sources...
  17. ncbi request reprint Genetic manipulation of Leptospira biflexa
    Hélène Louvel
    Unité de Biologie des Spirochètes Institut Pasteur, Paris, France
    Curr Protoc Microbiol . 2007
    ..biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes...
  18. pmc Comparative and functional genomic analyses of iron transport and regulation in Leptospira spp
    H Louvel
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 188:7893-904. 2006
    ..biflexa. Comparative genomics, in combination with gene inactivation, gives us significant functional information on iron homeostasis in Leptospira spp...
  19. pmc Human leptospira isolates circulating in Mayotte (Indian Ocean) have unique serological and molecular features
    P Bourhy
    Institut Pasteur, Unité de Biologie des Spirochètes, National Reference Centre for Leptospirosis, WHO Collaborating Center for the Epidemiology of Leptospirosis, Paris, France
    J Clin Microbiol 50:307-11. 2012
    ..These results are essential for the improvement of current diagnostic tools and provide a starting point for a better understanding of the epidemiology of leptospirosis in this area of endemicity...
  20. doi request reprint Transposition of fly mariner elements into bacteria as a genetic tool for mutagenesis
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Genetica 138:551-8. 2010
    ..Transposon-based approaches greatly facilitate studies of bacterial biology. We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria...
  21. ncbi request reprint Construction and complementation of the first auxotrophic mutant in the spirochaete Leptospira meyeri
    Hélène Bauby
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 149:689-93. 2003
    ..The authors have also characterized a second selectable marker that allows the construction of a spectinomycin-resistant L. meyeri-E. coli shuttle vector and the functional complementation of the L. meyeri trpE mutant...
  22. doi request reprint Diagnosis and epidemiology of leptospirosis
    M Picardeau
    Institut Pasteur, Unité de Biologie des Spirochètes, centre national de référence et centre collaborateur de l OMS de la leptospirose, 28, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Med Mal Infect 43:1-9. 2013
    ..Leptospirosis is expected to become more important due to a rapid urbanization in developing countries (slums), global warming, and extreme climatic events (floods)...
  23. pmc Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto
    Z Majed
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 43:539-45. 2005
    ..interrogans. This rapid and easy method should greatly contribute to a better knowledge of the epidemiology of Leptospira...
  24. pmc Random insertional mutagenesis of Leptospira interrogans, the agent of leptospirosis, using a mariner transposon
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3255-8. 2005
    ..interrogans mutants. This approach of transposon mutagenesis will be useful for understanding the spirochetal physiology and the pathogenic mechanisms of Leptospira, which remain largely unknown...
  25. pmc Isolation and characterization of new Leptospira genotypes from patients in Mayotte (Indian Ocean)
    Pascale Bourhy
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Negl Trop Dis 4:e724. 2010
    ..To date, Leptospira isolates have never been isolated in this endemic region...
  26. doi request reprint Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis
    Kristel Lourdault
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    J Med Microbiol 58:648-55. 2009
    ..qPCR will be used in future studies to evaluate the efficacy of vaccine candidates against leptospirosis and the virulence of selected L. interrogans mutants relative to the parental strain...
  27. pmc The scc spirochetal coiled-coil protein forms helix-like filaments and binds to nucleic acids generating nucleoprotein structures
    Khalil Mazouni
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 188:469-76. 2006
    ..These results provide the first insight into a unique DNA binding filament-forming coiled-coil protein that could play an important role in the subcellular architecture of the spirochetal microorganism...
  28. pmc Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology
    Ange Patricia Tchamedeu Kameni
    Unité de bactériologie moléculaire et médicale, Institut Pasteur, Paris, France
    J Bacteriol 184:452-8. 2002
    ..e., DNA is condensed at the midcell. Our data indicate that L. biflexa RecA plays a major role in ensuring cell viability via mechanisms such as DNA repair and, indirectly, active chromosome partitioning...
  29. pmc Antibiotic resistance markers for genetic manipulations of Leptospira spp
    Dante Poggi
    Unité de Biologie des Spirochètes, Institut Pasteur, 75724 Paris Cedex 15, France
    Appl Environ Microbiol 76:4882-5. 2010
    ..We also generated a gentamicin resistance cassette that allows the use of a third selectable marker in leptospires. These results may facilitate further advances in gene transfer systems in Leptospira spp...
  30. pmc The OmpA-like protein Loa22 is essential for leptospiral virulence
    Paula Ristow
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS Pathog 3:e97. 2007
    ..Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira species...
  31. pmc Expanding the genetic toolbox for Leptospira species by generation of fluorescent bacteria
    Florence Aviat
    Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France
    Appl Environ Microbiol 76:8135-42. 2010
    ..Finally, we showed that GFP can be used as a reporter to assess promoter activity in different environmental conditions. These results may facilitate further advances for studying the genetics of Leptospira spp...
  32. pmc Complete nucleotide sequence of the LE1 prophage from the spirochete Leptospira biflexa and characterization of its replication and partition functions
    Pascale Bourhy
    Laboratoire des Spirochètes, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 187:3931-40. 2005
    ..These results enable the construction of new shuttle vectors for studying the genetics of Leptospira spp. This study will also contribute to a better knowledge of phages unrelated to lambdoid phages...
  33. doi request reprint Biofilm formation by saprophytic and pathogenic leptospires
    Paula Ristow
    Unité de Biologie des Spirochètes, Institut Pasteur, 75724 Paris Cedex 15, France
    Microbiology 154:1309-17. 2008
    ..The formation of such a biofilm is consistent with the life of saprophytic strains in water and may help pathogenic strains to survive in environmental habitats and to colonize the host...
  34. pmc Genome sequence of the saprophyte Leptospira biflexa provides insights into the evolution of Leptospira and the pathogenesis of leptospirosis
    Mathieu Picardeau
    Unité de Biologie des Spirochètes, Institut Pasteur, Paris, France
    PLoS ONE 3:e1607. 2008
    ..borgpetersenii has impaired its survival outside a mammalian host, whereas L. interrogans has retained environmental sensory functions that facilitate disease transmission through water...
  35. pmc Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of leptospirosis
    Laurence Salaun
    Laboratoire des Spirochètes, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 44:3954-62. 2006
    ..Our results show that this simple PCR-based MLVA typing technique is a powerful methodology for the epidemiology of leptospirosis...
  36. ncbi request reprint A comprehensive survey on isoleucine biosynthesis pathways in seven epidemic Leptospira interrogans reference strains of China
    Ying Zou
    Laboratory of Molecular Microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
    FEMS Microbiol Lett 269:90-6. 2007
    ....