A Phalipon

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. ncbi request reprint Shigellosis: innate mechanisms of inflammatory destruction of the intestinal epithelium, adaptive immune response, and vaccine development
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France
    Crit Rev Immunol 23:371-401. 2003
  2. ncbi request reprint Induction of anti-carbohydrate antibodies by phage library-selected peptide mimics
    A Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    Eur J Immunol 27:2620-5. 1997
  3. ncbi request reprint Secretory component: a new role in secretory IgA-mediated immune exclusion in vivo
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U 389, France
    Immunity 17:107-15. 2002
  4. ncbi request reprint Shigella's ways of manipulating the host intestinal innate and adaptive immune system: a tool box for survival?
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U786, Institut Pasteur 25, rue du Dr Roux, Paris, France
    Immunol Cell Biol 85:119-29. 2007
  5. ncbi request reprint Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 389
    J Immunol 176:1686-94. 2006
  6. pmc Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody
    B Vulliez-le Normand
    Institut Pasteur, Unité d Immunologie Structurale, Centre National de la Recherche Scientifique Unité de Recherche Associée 2185, F 75015 Paris, France
    Proc Natl Acad Sci U S A 105:9976-81. 2008
  7. pmc Immunogenicity of IpaC-hybrid proteins expressed in the Shigella flexneri 2a vaccine candidate SC602
    S Barzu
    Unite de Pathogenie Microbienne Moleculaire, U389 Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France
    Infect Immun 66:77-82. 1998
  8. doi request reprint A synthetic carbohydrate-protein conjugate vaccine candidate against Shigella flexneri 2a infection
    Armelle Phalipon
    Institut Pasteur, Unite de Pathogenie Microbienne Moleculaire, Paris, France
    J Immunol 182:2241-7. 2009
  9. ncbi request reprint A newborn mouse model for the study of intestinal pathogenesis of shigellosis
    M I Fernandez
    Unite de Pathogenie Microbienne Moleculaire, 28 rue du Dr Roux, 75015 Paris, France
    Cell Microbiol 5:481-91. 2003
  10. ncbi request reprint Anti-inflammatory role for intracellular dimeric immunoglobulin a by neutralization of lipopolysaccharide in epithelial cells
    M Isabel Fernandez
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, 28 rue du Dr Roux, 75015, Paris, France
    Immunity 18:739-49. 2003

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Shigellosis: innate mechanisms of inflammatory destruction of the intestinal epithelium, adaptive immune response, and vaccine development
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U389, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France
    Crit Rev Immunol 23:371-401. 2003
    ..This profile is likely to impact on the nature and quality of the adaptive response, which is dominated by humoral protection at the mucosal level...
  2. ncbi request reprint Induction of anti-carbohydrate antibodies by phage library-selected peptide mimics
    A Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    Eur J Immunol 27:2620-5. 1997
    ..These results are the first example of immunogenic mimicry of carbohydrates by phage-displayed peptides, and indicate a new strategy of selection of immunogens for the development of anti-polysaccharide vaccines...
  3. ncbi request reprint Secretory component: a new role in secretory IgA-mediated immune exclusion in vivo
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U 389, France
    Immunity 17:107-15. 2002
    ..Therefore, binding of IgA(d/p) to SC during the course of SIgA-mediated mucosal response constitutes a crucial step in achieving efficient protection of the epithelial barrier by immune exclusion...
  4. ncbi request reprint Shigella's ways of manipulating the host intestinal innate and adaptive immune system: a tool box for survival?
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U786, Institut Pasteur 25, rue du Dr Roux, Paris, France
    Immunol Cell Biol 85:119-29. 2007
    ..The escape strategies, the possible direct effect of Shigella on B and T lymphocytes, their impact on the development of adaptive immunity, and how they may help explain the limited protection induced by natural infection are discussed...
  5. ncbi request reprint Characterization of functional oligosaccharide mimics of the Shigella flexneri serotype 2a O-antigen: implications for the development of a chemically defined glycoconjugate vaccine
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut National de la Santé et de la Recherche Médicale Unité 389
    J Immunol 176:1686-94. 2006
    ..A pentadecasaccharide representing three biological repeating units was identified as a potential candidate for further development of a chemically defined glycoconjugate vaccine against S. flexneri 2a infection...
  6. pmc Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody
    B Vulliez-le Normand
    Institut Pasteur, Unité d Immunologie Structurale, Centre National de la Recherche Scientifique Unité de Recherche Associée 2185, F 75015 Paris, France
    Proc Natl Acad Sci U S A 105:9976-81. 2008
    ..Our results have implications for vaccine development because they suggest that a minimum of two RUs of synthetic serotype 2a oligosaccharide is required for optimal mimicry of O-Ag epitopes...
  7. pmc Immunogenicity of IpaC-hybrid proteins expressed in the Shigella flexneri 2a vaccine candidate SC602
    S Barzu
    Unite de Pathogenie Microbienne Moleculaire, U389 Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France
    Infect Immun 66:77-82. 1998
    ..flexneri live attenuated strains as mucosal vectors for foreign epitopes...
  8. doi request reprint A synthetic carbohydrate-protein conjugate vaccine candidate against Shigella flexneri 2a infection
    Armelle Phalipon
    Institut Pasteur, Unite de Pathogenie Microbienne Moleculaire, Paris, France
    J Immunol 182:2241-7. 2009
    ..This synthetic carbohydrate-based conjugate may be a candidate for a SF2a vaccine...
  9. ncbi request reprint A newborn mouse model for the study of intestinal pathogenesis of shigellosis
    M I Fernandez
    Unite de Pathogenie Microbienne Moleculaire, 28 rue du Dr Roux, 75015 Paris, France
    Cell Microbiol 5:481-91. 2003
    ..In contrast, 5-day-old mice inoculated with either the invasive or the non-invasive strain were not infected. We have thus established a mouse model that is suitable for the study of the pathogenesis of intestinal Shigella infection...
  10. ncbi request reprint Anti-inflammatory role for intracellular dimeric immunoglobulin a by neutralization of lipopolysaccharide in epithelial cells
    M Isabel Fernandez
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, 28 rue du Dr Roux, 75015, Paris, France
    Immunity 18:739-49. 2003
    ..Thus, intracellular neutralization by dIgA limits the acute local inflammation induced by proinflammatory pathogen-associated molecular patterns such as LPS...
  11. pmc Dipstick for rapid diagnosis of Shigella flexneri 2a in stool
    Faridabano Nato
    Plate Forme 5 Production de Protéines Recombinantes et d Anticorps, Institut Pasteur, Paris, France
    PLoS ONE 2:e361. 2007
    ..Here, we describe a new diagnostic test for rapid detection, in stool, at the bedside of patients, of Shigella flexneri 2a, the most predominant agent of the endemic form of the disease...
  12. doi request reprint Vaccination against shigellosis: is it the path that is difficult or is it the difficult that is the path?
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, 75724 Paris Cedex 15, France
    Microbes Infect 10:1057-62. 2008
    ..More basic research is also required to improve what we can still consider as first-generation vaccines, and to explore possible new paradigms including the search for cross-protective antigens...
  13. ncbi request reprint Blockwise approach to fragments of the O-specific polysaccharide of Shigella flexneri serotype 2a: convergent Synthesis of a decasaccharide representative of a dimer of the branched repeating unit
    Frederic Belot
    Unite de Chimie Organique, URA CNRS 2128, Inserm 389, Institut Pasteur, Paris, France
    J Org Chem 69:1060-74. 2004
    ..It is based on the use of the trichloroacetimidate chemistry. The target branched oligosaccharide was designed to probe the recognition at the molecular level of the natural polysaccharide by protective monoclonal antibodies...
  14. doi request reprint Structural mimicry of O-antigen by a peptide revealed in a complex with an antibody raised against Shigella flexneri serotype 2a
    François Xavier Theillet
    Institut Pasteur, Unité de RMN des Biomolécules, CNRS URA 2185, Paris, France
    J Mol Biol 388:839-50. 2009
    ....
  15. ncbi request reprint MxiG, a membrane protein required for secretion of Shigella spp. Ipa invasins: involvement in entry into epithelial cells and in intercellular dissemination
    A Allaoui
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    Mol Microbiol 17:461-70. 1995
    ..Therefore, MxiG and possibly proteins secreted by the Mxi-Spa translocation are involved not only in entry but also in spread of Shigella between epithelial cells...
  16. ncbi request reprint Toward a better understanding of the basis of the molecular mimicry of polysaccharide antigens by peptides: the example of Shigella flexneri 5a
    Marie Jeanne Clément
    Unité de RMN des Biomolécules, URA CNRS 2185, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    J Biol Chem 281:2317-32. 2006
    ..This newly made available system provides a new contribution to the understanding of the molecular mimicry of complex polysaccharides by peptides and short oligosaccharides...
  17. ncbi request reprint Conformational studies of the O-specific polysaccharide of Shigella flexneri 5a and of four related synthetic pentasaccharide fragments using NMR and molecular modeling
    Marie Jeanne Clément
    Unité de RMN des Biomolécules, URA CNRS 2185, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France
    J Biol Chem 278:47928-36. 2003
    ..The models that are in agreement with NMR data adopt right-handed 3-fold helical structures in which the branched glucosyl residue points outwards...
  18. ncbi request reprint Novel functions of the polymeric Ig receptor: well beyond transport of immunoglobulins
    Armelle Phalipon
    Unite de Pathogenie Microbienne Moleculaire, INSERM U 389, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France
    Trends Immunol 24:55-8. 2003
    ..The multiple mechanisms that they are involved in make pIgR and SC instrumental to mucosal immunity...
  19. doi request reprint Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity
    Gernot Sellge
    Unite de Pathogenie Microbienne Moleculaire, Institut Pasteur, Paris, France
    J Immunol 184:2076-85. 2010
    ..These findings bring new insights into the adaptive immune response to Shigella infection and highlight the importance of pathogen-specific Th17 cell immunity for secondary immune protection...
  20. pmc Differential activation of human TLR4 by Escherichia coli and Shigella flexneri 2a lipopolysaccharide: combined effects of lipid A acylation state and TLR4 polymorphisms on signaling
    Prasad Rallabhandi
    Department of Microbiology and Immunology, Center for Vaccine Development, University of Maryland, Baltimore 21201, USA
    J Immunol 180:1139-47. 2008
    ..Thus, the combination of acylation state and host genetics may significantly impact vaccine immunogenicity and/or efficacy, whether LPS is an integral component of a whole organism vaccine or included as an adjuvant...
  21. ncbi request reprint Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental shigellosis
    Maria Celeste Martino
    Dipartimento di Biologia Cellulare e dello Sviluppo, Universita La Sapienza, Rome, Italy
    J Infect Dis 192:136-48. 2005
    ..Because mice are naturally resistant to shigellosis, there is no mouse model that mimics human disease. We explore the susceptibility of intestinal flora-depleted mice to shigellosis after intragastric infection with Shigella strains...
  22. ncbi request reprint Cytotoxicity and interleukin-1beta processing following Shigella flexneri infection of human monocyte-derived dendritic cells
    Jonathan D Edgeworth
    Department of Infectious Diseases, St George s Hospital Medical School, London, GB
    Eur J Immunol 32:1464-71. 2002
    ..The rapid death of DC during the early stages of shigellosis is likely to have adverse consequences for generation of adaptive immunity...
  23. ncbi request reprint Roles for T and NK cells in the innate immune response to Shigella flexneri
    Karine Le-Barillec
    Unité des Cytokines et Développement Lymphoide, Institut National de la Sante et de la Recherche Medicale, Unité 668, Paris Cedex, France
    J Immunol 175:1735-40. 2005
    ..flexneri infection through amplification of an inflammatory response. This cellular lymphocyte redundancy assures IFN-gamma production, which is central to innate immunity against Shigella infection...
  24. ncbi request reprint Delivery of biologically active anti-inflammatory cytokines IL-10 and IL-1ra in vivo by the Shigella type III secretion apparatus
    Mustapha Chamekh
    Laboratoire de Bactériologie Moléculaire, Faculte de Medecine, Universite Libre de Bruxelles, Brussels, Belgium
    J Immunol 180:4292-8. 2008
    ..These findings demonstrate that the Shigella T3S apparatus can deliver biologically active cytokines in vivo, thus opening new avenues for the use of attenuated bacteria to deliver proteins for immunomodulation or gene therapy purposes...