Franck Perez

Summary

Affiliation: Institut Curie
Country: France

Publications

  1. pmc CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family
    Franck Perez
    Institut Curie, CNRS UMR144, 75248 Paris, France
    J Cell Biol 156:631-42. 2002
  2. ncbi request reprint Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking
    Elaine del Nery
    Molecular Mechanisms of Intracellular Transport, UMR CNRS 144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Traffic 7:394-407. 2006
  3. pmc Characterization of single chain antibody targets through yeast two hybrid
    Ole Vielemeyer
    Institut Curie Research Center, 26 Rue d Ulm, Paris Cedex 05, France
    BMC Biotechnol 10:59. 2010
  4. ncbi request reprint Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo
    Clement Nizak
    CNRS UMR144, Institut Curie, 26 rue d Ulm 75248 Paris Cedex 05 France
    Traffic 4:739-53. 2003
  5. doi request reprint Synchronization of secretory protein traffic in populations of cells
    Gaelle Boncompain
    Institut Curie, Centre de Recherche, Paris, France
    Nat Methods 9:493-8. 2012
  6. pmc A multi-Fc-species system for recombinant antibody production
    Sandrine Moutel
    CNRS UMR144, 26 Rue d Ulm, F75248 Paris Cedex 05, France
    BMC Biotechnol 9:14. 2009
  7. doi request reprint Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues
    Ariane Dimitrov
    CNRS UMR144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Science 322:1353-6. 2008
  8. doi request reprint Selection and use of intracellular antibodies (intrabodies)
    Sandrine Moutel
    Institut Curie, CNRS UMR144, Paris, France
    Methods Mol Biol 907:667-79. 2012
  9. doi request reprint Fully in vitro selection of recombinant antibodies
    Sandrine Moutel
    Transfer Department, Institut Curie, Paris, France
    Biotechnol J 4:38-43. 2009
  10. pmc Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping
    Ole Vielemeyer
    Research Section, Translational Department, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 5, France
    J Biol Chem 284:20791-5. 2009

Collaborators

Detail Information

Publications30

  1. pmc CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family
    Franck Perez
    Institut Curie, CNRS UMR144, 75248 Paris, France
    J Cell Biol 156:631-42. 2002
    ..However, CLIPR-59 overexpression strongly perturbs early/recycling endosome-TGN dynamics, implicating CLIPR-59 in the regulation of this pathway...
  2. ncbi request reprint Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking
    Elaine del Nery
    Molecular Mechanisms of Intracellular Transport, UMR CNRS 144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Traffic 7:394-407. 2006
    ..Thus, our work shows that Rab6A and Rab6A' perform different functions within the cell and suggests a novel role for Rab6A' as the major Rab6 isoform regulating previously described Rab6-dependent transport pathways...
  3. pmc Characterization of single chain antibody targets through yeast two hybrid
    Ole Vielemeyer
    Institut Curie Research Center, 26 Rue d Ulm, Paris Cedex 05, France
    BMC Biotechnol 10:59. 2010
    ..However, such data is frequently difficult to obtain...
  4. ncbi request reprint Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo
    Clement Nizak
    CNRS UMR144, Institut Curie, 26 rue d Ulm 75248 Paris Cedex 05 France
    Traffic 4:739-53. 2003
    ..Altogether, we show that the antibody phage display technique represents an efficient alternative to rapidly generate versatile antibodies that represent new tools to study protein function...
  5. doi request reprint Synchronization of secretory protein traffic in populations of cells
    Gaelle Boncompain
    Institut Curie, Centre de Recherche, Paris, France
    Nat Methods 9:493-8. 2012
    ..The system should help increase the understanding of the mechanisms of trafficking and enable screens for molecules that perturb pathological protein transport...
  6. pmc A multi-Fc-species system for recombinant antibody production
    Sandrine Moutel
    CNRS UMR144, 26 Rue d Ulm, F75248 Paris Cedex 05, France
    BMC Biotechnol 9:14. 2009
    ..However, so far, recombinant antibodies have not managed to impose themselves as efficient alternatives to natural antibodies...
  7. doi request reprint Detection of GTP-tubulin conformation in vivo reveals a role for GTP remnants in microtubule rescues
    Ariane Dimitrov
    CNRS UMR144, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Science 322:1353-6. 2008
    ..Observations in living cells suggested that these GTP remnants may be responsible for the rescue events in which microtubules recover from catastrophe...
  8. doi request reprint Selection and use of intracellular antibodies (intrabodies)
    Sandrine Moutel
    Institut Curie, CNRS UMR144, Paris, France
    Methods Mol Biol 907:667-79. 2012
    ..We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies...
  9. doi request reprint Fully in vitro selection of recombinant antibodies
    Sandrine Moutel
    Transfer Department, Institut Curie, Paris, France
    Biotechnol J 4:38-43. 2009
    ....
  10. pmc Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping
    Ole Vielemeyer
    Research Section, Translational Department, Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 5, France
    J Biol Chem 284:20791-5. 2009
    ....
  11. doi request reprint Imaging GTP-bound tubulin: from cellular to in vitro assembled microtubules
    Hélène de Forges
    Institut Curie, Paris Cedex 05, France
    Methods Cell Biol 115:139-53. 2013
    ..In this chapter, we recapitulate essential tools to study the GTP-tubulin using the recombinant antibody MB11 from permeabilized cells to in vitro assays...
  12. ncbi request reprint ESCRT machinery is required for plasma membrane repair
    Ana Joaquina Jimenez
    Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Science 343:1247136. 2014
    ..Thus, the repair of certain wounds is ensured by ESCRT-mediated extracellular shedding of wounded portions. ..
  13. doi request reprint Synchronizing protein transport in the secretory pathway
    Gaelle Boncompain
    Research Center, Institut Curie, Paris, France
    Curr Protoc Cell Biol . 2012
    ..The use of the RUSH system is detailed in these protocols, from sub-cloning the sequence coding for the protein of interest into RUSH plasmids to visualization of its trafficking...
  14. pmc A role for the Rab6A' GTPase in the inactivation of the Mad2-spindle checkpoint
    Stéphanie Miserey-Lenkei
    UMR 144 CNRS IC, Institut Curie, Paris, France
    EMBO J 25:278-89. 2006
    ..Rab6A', through its interaction with p150(Glued) and GAPCenA, may thus participate in a pathway involved in the metaphase/anaphase transition...
  15. ncbi request reprint Golgi inheritance under a block of anterograde and retrograde traffic
    Clement Nizak
    Institut Curie, CNRS UMR144, 26 rue d Ulm 75248 Paris Cedex 05, France
    Traffic 5:284-99. 2004
    ....
  16. doi request reprint [Intrabodies, potent tools to unravel the function and dynamics of intracellular proteins]
    Sandrine Moutel
    CNRS UMR144, Compartimentation et Dynamique Cellulaires, département de recherche translationnelle, Institut Curie, 26, rue d Ulm, 75248 Paris Cedex 05, France
    Med Sci (Paris) 25:1173-6. 2009
    ..Intrabodies not only represent new tools for fundamental research to study the dynamics of endogenous proteins, but may also bring interesting options for applied research in terms of intracellular immunization for therapeutic use...
  17. doi request reprint The gene responsible for Dyggve-Melchior-Clausen syndrome encodes a novel peripheral membrane protein dynamically associated with the Golgi apparatus
    Ariane Dimitrov
    UMR CNRS 144, Institut Curie, 75005 Paris, France
    Hum Mol Genet 18:440-53. 2009
    ..These data indicate that DMC results from a loss-of-function of Dymeclin, a novel peripheral membrane protein which shuttles rapidly between the cytosol and mature Golgi membranes and point out a role of Dymeclin in cellular trafficking...
  18. doi request reprint Interplay between microtubule dynamics and intracellular organization
    Hélène de Forges
    Institut Curie, 26 Rue d Ulm, 75248 Paris Cedex 05, France
    Int J Biochem Cell Biol 44:266-74. 2012
    ..In these cells, the microtubule network is reorganized and undergoes specific and local regulation events; microtubules also participate in the intracellular reorganization of different organelles to ensure proper cell differentiation...
  19. ncbi request reprint Recombinant antibodies to the small GTPase Rab6 as conformation sensors
    Clement Nizak
    CNRS UMR144, Institut Curie, 26 Rue d Ulm, F75248 Paris Cedex 05, France
    Science 300:984-7. 2003
    ..More generally, the same approach could be applied to other molecules that can be locked in a particular conformation in vitro...
  20. ncbi request reprint Synchronization of secretory cargos trafficking in populations of cells
    Gaelle Boncompain
    Institut Curie, CNRS UMR144, 26 Rue d Ulm, Paris Cedex 05, 75248, France
    Methods Mol Biol 1174:211-23. 2014
    ..Here, we describe how to subclone the gene coding for a cargo of interest into a RUSH plasmid and to monitor its synchronized transport along the secretory pathway in fixed samples and in living cells. ..
  21. doi request reprint Fluorescence-based analysis of trafficking in mammalian cells
    Gaelle Boncompain
    Institut Curie, Paris, France CNRS UMR144, Paris, France
    Methods Cell Biol 118:179-94. 2013
    ..Analysis of the transport steps of the cargos from the donor compartment to the acceptor compartment is accomplished by fluorescence-based methods. ..
  22. doi request reprint The many routes of Golgi-dependent trafficking
    Gaelle Boncompain
    Institut Curie, Paris, France
    Histochem Cell Biol 140:251-60. 2013
    ..We will review here the main characteristics of intra-Golgi trafficking discuss the different models that were built to explain the diversity of intra-Golgi routes and its dynamics and propose an integrative model. ..
  23. doi request reprint "Antibodies--Europe. Engineering the next generation of antibodies"
    Sandrine Moutel
    Institut Curie, Paris, France
    Biotechnol J 3:298-300. 2008
  24. ncbi request reprint Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170
    Masaki Fukata
    Department of Cell Pharmacology, Nagoya University, Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya, Aichi 466 8550, Japan
    Cell 109:873-85. 2002
    ..These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization...
  25. ncbi request reprint CLIPR-59 is a lipid raft-associated protein containing a cytoskeleton-associated protein glycine-rich domain (CAP-Gly) that perturbs microtubule dynamics
    Valérie Lallemand-Breitenbach
    CNRS Unité Mixte de Recherche 144, Institut Curie Section Recherche, 75248 Paris Cedex 05, France
    J Biol Chem 279:41168-78. 2004
    ..In contrast with the role of linker between membranes and microtubules that was proposed for CLIP function, CLIPR-59 thus may have an "anti-CLIP" function by preventing microtubule-raft interactions...
  26. ncbi request reprint Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells
    Ugur Sahin
    Medizinische Klinik I, Saarland University Medical School, Homburg, Germany
    Blood 100:4139-45. 2002
    ..The strong expression of CLIP-170/restin in HRS cells, DCs, and activated B cells underscores their functional similarities supporting a function-based concept of HRS cells as professional antigen-presenting cells...
  27. ncbi request reprint A plus-end raft to control microtubule dynamics and function
    Niels Galjart
    Department of Cell Biology and Genetics, Erasmus University, PO Box 1738, 3000 DR Rotterdam, The Netherlands
    Curr Opin Cell Biol 15:48-53. 2003
    ..This may, in turn, control the dynamic behaviour of this cytoskeletal network and its anchoring to other structures...
  28. pmc Analysis of de novo Golgi complex formation after enzyme-based inactivation
    Florence Jollivet
    Centre National de la Recherche Scientifique Unité Mixte de Recherche 144, 75248 Paris Cedex 05, France
    Mol Biol Cell 18:4637-47. 2007
    ....
  29. pmc Protein interaction mapping: a Drosophila case study
    Etienne Formstecher
    Hybrigenics, 75014 Paris, France
    Genome Res 15:376-84. 2005
    ..Analysis of the differences in data sets and methods suggests alternative strategies to enhance the accuracy and comprehensiveness of the post-genomic generation of broad-scale protein interaction maps...
  30. ncbi request reprint Kinesin is involved in protecting nascent microtubules from disassembly after recovery from nocodazole treatment
    Jérôme Marceiller
    Laboratoire de Biochimie et Biologie Cellulaire, EAD 1595, Faculte de Pharmacie, 5 rue J B Clement, 92296 Chatenay Malabry Cedex, France
    Exp Cell Res 304:483-92. 2005
    ..Altogether, our findings suggest that kinesin is involved in an early microtubule protection process that is linked to the control of their dynamics during their early growth phase...