L M Mir

Summary

Affiliation: Institut Gustave Roussy
Country: France

Publications

  1. doi request reprint Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future
    L M Mir
    Centre National de la Recherche Scientifique, UMR 8121, Institut Gustave Roussy, 39 Rue C Desmoulins, Villejuif Cedex, France
    Mol Biotechnol 43:167-76. 2009
  2. pmc In vivo evolution of tumour cells after the generation of double-strand DNA breaks
    H Mekid
    , UMR 8121 CNRS, Institut Gustave Roussy, 39 Rue C Desmoulins, , France
    Br J Cancer 88:1763-71. 2003
  3. pmc Tumor ablation with irreversible electroporation
    Bassim Al-Sakere
    CNRS UMR 8121, Institut Gustave Roussy, Villejuif, France
    PLoS ONE 2:e1135. 2007
  4. ncbi request reprint In vivo MRI follow-up of murine tumors treated by electrochemotherapy and other electroporation-based treatments
    L Calmels
    Univ Paris Sud, IR4M, UMR 8081 2CNRS, IR4M, UMR 8081, Orsay, 91405, France
    Technol Cancer Res Treat 11:561-70. 2012
  5. ncbi request reprint A study of the immunological response to tumor ablation with irreversible electroporation
    B Al-Sakere
    UMR 8121 CNRS Institut, Gustave Roussy, 39 Rue C Desmoulins, F 94805 Villejuif, France
    Technol Cancer Res Treat 6:301-6. 2007
  6. pmc Treatment of cancer with cryochemotherapy
    L M Mir
    FRE 2530 CNRS, Institut Gustave Roussy, PR II, 39, rue Camille Desmoulins, F 94805 Villejuif Cedex, France
    Br J Cancer 86:1658-60. 2002
  7. ncbi request reprint Therapeutic perspectives of in vivo cell electropermeabilization
    L M Mir
    Laboratoire de Physicochimie et Pharmacologie des Macromolecules Biologiques, UMR 8532 CNRS, Institut Gustave Roussy, Villejuif, France
    Bioelectrochemistry 53:1-10. 2001
  8. pmc High-efficiency gene transfer into skeletal muscle mediated by electric pulses
    L M Mir
    Unité Mixte de Recherche 8532 Centre National de la Recherche Scientifique, Institut Gustave Roussy, 39 Rue C Desmoulins, F 94805 Villejuif Cedex, France
    Proc Natl Acad Sci U S A 96:4262-7. 1999
  9. ncbi request reprint Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle
    L M Mir
    UMR 1772 CNRS, Institut Gustave Roussy, Villejuif, France
    C R Acad Sci III 321:893-9. 1998
  10. doi request reprint Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin
    F M André
    CNRS, UMR 8121, Institute Gustave Roussy, F 94805 Villejuif Cedex, France
    Hum Gene Ther 19:1261-71. 2008

Collaborators

Detail Information

Publications25

  1. doi request reprint Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future
    L M Mir
    Centre National de la Recherche Scientifique, UMR 8121, Institut Gustave Roussy, 39 Rue C Desmoulins, Villejuif Cedex, France
    Mol Biotechnol 43:167-76. 2009
    ..A good understanding of DNA electrotransfer principles and the respect of safe procedures will be key elements for a successful future transfer DNA electrotransfer into the clinics...
  2. pmc In vivo evolution of tumour cells after the generation of double-strand DNA breaks
    H Mekid
    , UMR 8121 CNRS, Institut Gustave Roussy, 39 Rue C Desmoulins, , France
    Br J Cancer 88:1763-71. 2003
    ..These cell death pathways are discussed as a function of the number of DSB generated, by comparison with previous results obtained in vitro using BLM or ionising radiation...
  3. pmc Tumor ablation with irreversible electroporation
    Bassim Al-Sakere
    CNRS UMR 8121, Institut Gustave Roussy, Villejuif, France
    PLoS ONE 2:e1135. 2007
    ..These conditions induced complete regression in 12 out of 13 treated tumors, (92%), in the absence of tissue heating. Irreversible electroporation is thus a new effective modality for non-thermal tumor ablation...
  4. ncbi request reprint In vivo MRI follow-up of murine tumors treated by electrochemotherapy and other electroporation-based treatments
    L Calmels
    Univ Paris Sud, IR4M, UMR 8081 2CNRS, IR4M, UMR 8081, Orsay, 91405, France
    Technol Cancer Res Treat 11:561-70. 2012
    ..Noteworthy, ADC allowed the visualization of early and rapid changes in the treated tumors, when tumor volume monitoring was not yet able to detect any effect of the treatments...
  5. ncbi request reprint A study of the immunological response to tumor ablation with irreversible electroporation
    B Al-Sakere
    UMR 8121 CNRS Institut, Gustave Roussy, 39 Rue C Desmoulins, F 94805 Villejuif, France
    Technol Cancer Res Treat 6:301-6. 2007
    ..We confirm here that immune response is not instrumental in irreversible electroporation efficacy, and we propose that irreversible electroporation may be, therefore, a treatment modality of interest to immunodepressed cancer patients...
  6. pmc Treatment of cancer with cryochemotherapy
    L M Mir
    FRE 2530 CNRS, Institut Gustave Roussy, PR II, 39, rue Camille Desmoulins, F 94805 Villejuif Cedex, France
    Br J Cancer 86:1658-60. 2002
    ..We report that freezing facilitates bleomycin penetration into cells making it toxic to cryosurgery surviving cells at concentrations that are non-toxic systemically...
  7. ncbi request reprint Therapeutic perspectives of in vivo cell electropermeabilization
    L M Mir
    Laboratoire de Physicochimie et Pharmacologie des Macromolecules Biologiques, UMR 8532 CNRS, Institut Gustave Roussy, Villejuif, France
    Bioelectrochemistry 53:1-10. 2001
    ..Present knowledge on DNA electrotransfer mechanisms, as wel as the potentialities of DNA electrotransfer to become an efficient non-viral approach for gene therapy, are reviewed...
  8. pmc High-efficiency gene transfer into skeletal muscle mediated by electric pulses
    L M Mir
    Unité Mixte de Recherche 8532 Centre National de la Recherche Scientifique, Institut Gustave Roussy, 39 Rue C Desmoulins, F 94805 Villejuif Cedex, France
    Proc Natl Acad Sci U S A 96:4262-7. 1999
    ..DNA electrotransfer in muscle may have broad applications in gene therapy and in physiological, pharmacological, and developmental studies...
  9. ncbi request reprint Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle
    L M Mir
    UMR 1772 CNRS, Institut Gustave Roussy, Villejuif, France
    C R Acad Sci III 321:893-9. 1998
    ..Therefore, DNA electrotransfer in muscle possesses broad potential applications in gene therapy and for physiological, pharmacological and developmental studies...
  10. doi request reprint Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin
    F M André
    CNRS, UMR 8121, Institute Gustave Roussy, F 94805 Villejuif Cedex, France
    Hum Gene Ther 19:1261-71. 2008
    ..In conclusion, HV plus LV pulses represent an efficient and safe option for future clinical trials and we suggest recommendations for gene transfer to various types of tissues...
  11. doi request reprint Canstatin gene electrotransfer combined with radiotherapy: preclinical trials for cancer treatment
    C Magnon
    CNRS, UMR8121, Vectorologie et Transfert de Gènes, Villejuif, Cedex, France
    Gene Ther 15:1436-45. 2008
    ..This extremely simple virus free curative protocol could open the door to potential clinical applications, especially for prostate cancer that often develops radioresistance...
  12. pmc Electrochemotherapy on liver tumours in rabbits
    L H Ramirez
    UMR 1772 CNRS, Institut Gustave Roussy, Villejuif, France
    Br J Cancer 77:2104-11. 1998
    ..Thus, ECT could become a novel treatment modality for liver tumours and other solid internal malignancies...
  13. ncbi request reprint Variability of naked DNA expression after direct local injection: the influence of the injection speed
    F M André
    CNRS, UMR 8121, Laboratory of Vectorology and Gene Transfer, Institut Gustave Roussy, Universite Paris XI, 39 rue Camille Desmoulins, F 94805 Villejuif Cedex, France
    Gene Ther 13:1619-27. 2006
    ..There is a limited muscle cell stress independent of the injection speed. Heterogeneity in the injection speed might thus be an explanation for the variability in DNA expression after simple injection...
  14. ncbi request reprint Treatment of muscle injuries by local administration of autologous conditioned serum: a pilot study on sportsmen with muscle strains
    T Wright-Carpenter
    UMR 8121 CNRS, Institut Gustave Roussy, Villejuif, France
    Int J Sports Med 25:588-93. 2004
    ..001). MRI analysis supported the observed acceleration of the lesion recovery time. We conclude that ACS injection is a promising approach to reduce the time to recovery from muscle injury...
  15. ncbi request reprint DNA electrotransfer: its principles and an updated review of its therapeutic applications
    F Andre
    Laboratory of Vectorology and Gene Transfer, UMR 8121 CNRS Institut Gustave Roussy, Villejuif Cedex, France
    Gene Ther 11:S33-42. 2004
    ..A summary of the main applications published until now is also reported, restricted to the in vivo preclinical trials using therapeutic genes...
  16. pmc Effective treatment of cutaneous and subcutaneous malignant tumours by electrochemotherapy
    L M Mir
    LPPMB Biochimie Enzymologie, URA 147 CNRS, Institute Gustave Roussy, Villejuif, France
    Br J Cancer 77:2336-42. 1998
    ..ECT was effective regardless of the histological type of the tumour. Therefore, ECT offers an approach to the treatment of cutaneous and subcutaneous tumours in patients with minimal adverse side-effects and with a high response rate...
  17. ncbi request reprint Treatment of muscle injuries by local administration of autologous conditioned serum: animal experiments using a muscle contusion model
    T Wright-Carpenter
    UMR 8121 CNRS, Institut Gustave Roussy, Villejuif, France
    Int J Sports Med 25:582-7. 2004
    ..In terms of clinical targets, this new approach could be used in the treatment of sports injuries and may also be interesting in postoperative situations...
  18. pmc The ratio of single- to double-strand DNA breaks and their absolute values determine cell death pathway
    O Tounekti
    UMR 8532 CNRS, Institut Gustave Roussy, PR II 39, rue Camille Desmoulins, , France
    Br J Cancer 84:1272-9. 2001
    ..Taken together with previous studies on bleomycin, our data demonstrates that cells can die by apoptosis, mitotic cell death, or pseudoapoptosis, depending on the number of DNA breaks and on the ratio of SSB to DSB...
  19. pmc First clinical trial of cat soft-tissue sarcomas treatment by electrochemotherapy
    L M Mir
    LPPMB URA 147 CNRS, Institut Gustave Roussy, Villejuif, France
    Br J Cancer 76:1617-22. 1997
    ..Electrochemotherapy is clearly effective as a salvage treatment for large spontaneous solid tumours in adverse clinical situations and this is promising for future applications...
  20. ncbi request reprint Electrochemotherapy: a new treatment of solid tumors
    L M Mir
    UMR 8121 CNRS, Institut Gustave Roussy, Villejuif, Cedex, France
    J Exp Clin Cancer Res 22:145-8. 2003
    ..Finally, future perspectives are discussed...
  21. ncbi request reprint Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation
    F X Barre
    Cancer, CNRS UPR9079 Institut Gustave Roussy, Villejuif, France
    Biotechniques 25:294-6. 1998
    ..This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide...
  22. doi request reprint Optimization of a gene electrotransfer method for mesenchymal stem cell transfection
    E Ferreira
    Laboratoire de Recherches Orthopediques B2OA, CNRS UMR 7052, Paris, France
    Gene Ther 15:537-44. 2008
    ..Thus, this study demonstrates the feasibility of efficient transgene electrotransfer into MSCs while preserving cell viability and multipotency...
  23. ncbi request reprint The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy
    G Pucihar
    Laboratory of Biocybernetics, Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI 1000 Ljubljana, Slovenia
    Bioelectrochemistry 57:167-72. 2002
    ..3 kHz. On the basis of these results the possibilities for the clinical use of pulses with high repetition frequency in electrochemotherapy are considered...
  24. ncbi request reprint Slow accumulation of plasmid in muscle cells: supporting evidence for a mechanism of DNA uptake by receptor-mediated endocytosis
    S Satkauskas
    Department of Biology, Vytautas Magnus University, LT-3000 Kaunas, Lithuania
    Mol Ther 4:317-23. 2001
    ..Therefore, uptake proceeds for the entire time during which intact DNA is present in the extracellular compartment. Our results support evidence for a DNA uptake mechanism based on receptor-mediated endocytosis...
  25. ncbi request reprint Role of pulse shape in cell membrane electropermeabilization
    T Kotnik
    Faculty of Electrical Engineering, University of Ljubljana, SI 1000 Ljubljana, Slovenia
    Biochim Biophys Acta 1614:193-200. 2003
    ..We show that the results of these experiments can be explained on the basis of the time during which the pulse amplitude exceeds a certain critical value...