C Locht

Summary

Affiliation: Institut Pasteur de Lille
Country: France

Publications

  1. pmc Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis
    Louise H Lefrançois
    INRA, UMR ISP 1282 Infectiologie et Santé Publique, Nouzilly F 37380, France
    BMC Res Notes 6:55. 2013
  2. pmc Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission
    Elian Dupré
    Institut Pasteur de Lille, Center for Infection and Immunity, Lille, France
    BMC Microbiol 13:172. 2013
  3. doi request reprint New pertussis vaccination approaches: en route to protect newborns?
    Camille Locht
    Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Lille, France
    FEMS Immunol Med Microbiol 66:121-33. 2012
  4. doi request reprint The ins and outs of pertussis toxin
    Camille Locht
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Univ Lille Nord de France, France
    FEBS J 278:4668-82. 2011
  5. pmc Genomic island excisions in Bordetella petrii
    Melanie Lechner
    Lehrstuhl fur Mikrobiologie, Biozentrum, Universitat Wurzburg, Am Hubland, Wurzburg, Germany
    BMC Microbiol 9:141. 2009
  6. pmc Detection of small RNAs in Bordetella pertussis and identification of a novel repeated genetic element
    David Hot
    Center for Infection and Immunity of Lille, Institut Pasteur de Lille, France
    BMC Genomics 12:207. 2011
  7. ncbi request reprint Bordetella pertussis, molecular pathogenesis under multiple aspects
    C Locht
    INSERM U447, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019, Lille Cedex, France
    Curr Opin Microbiol 4:82-9. 2001
  8. doi request reprint [New antituberculosis vaccines]
    C Locht
    Inserm 1019, centre d infection et immunité de Lille, CNRS UMR9204, Universite Lille Nord de France, Institut Pasteur de Lille, 1, rue du Professeur Calmette, 59019 Lille Cedex, France
    Arch Pediatr 18:1023-7. 2011
  9. ncbi request reprint Molecular aspects of Bordetella pertussis pathogenesis
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM, Institut Pasteur de Lille, France
    Int Microbiol 2:137-44. 1999
  10. ncbi request reprint How a different look at latency can help to develop novel diagnostics and vaccines against tuberculosis
    Camille Locht
    INSERM U629, Lille, France
    Expert Opin Biol Ther 7:1665-77. 2007

Detail Information

Publications121 found, 100 shown here

  1. pmc Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis
    Louise H Lefrançois
    INRA, UMR ISP 1282 Infectiologie et Santé Publique, Nouzilly F 37380, France
    BMC Res Notes 6:55. 2013
    ..Little is known about the virulence factors for this pathogen. In this study we have developed a method to produce and purify the heparin-binding hemagglutinin (HBHA), a major adhesin of Mycobacteria, from a culture of Map...
  2. pmc Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission
    Elian Dupré
    Institut Pasteur de Lille, Center for Infection and Immunity, Lille, France
    BMC Microbiol 13:172. 2013
    ..PAS domains are small, ubiquitous sensing or regulatory domains. The function of the PAS domain in BvgS remains unknown...
  3. doi request reprint New pertussis vaccination approaches: en route to protect newborns?
    Camille Locht
    Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Lille, France
    FEMS Immunol Med Microbiol 66:121-33. 2012
    ..However, as promising as it may be, in order to protect infants against severe disease, a single approach may not be sufficient, and multiple strategies applied in a concerted fashion may ultimately be required...
  4. doi request reprint The ins and outs of pertussis toxin
    Camille Locht
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Univ Lille Nord de France, France
    FEBS J 278:4668-82. 2011
    ..The toxin is one of the most important virulence factors of B. pertussis and is a component of all current vaccines against whooping cough...
  5. pmc Genomic island excisions in Bordetella petrii
    Melanie Lechner
    Lehrstuhl fur Mikrobiologie, Biozentrum, Universitat Wurzburg, Am Hubland, Wurzburg, Germany
    BMC Microbiol 9:141. 2009
    ..These elements mainly encode accessory metabolic factors enabling this bacterium to grow on a large repertoire of aromatic compounds...
  6. pmc Detection of small RNAs in Bordetella pertussis and identification of a novel repeated genetic element
    David Hot
    Center for Infection and Immunity of Lille, Institut Pasteur de Lille, France
    BMC Genomics 12:207. 2011
    ..So far, little is known about sRNAs in Bordetella, and only very few sRNAs have been identified in the genome of Bordetella pertussis, the causative agent of whooping cough...
  7. ncbi request reprint Bordetella pertussis, molecular pathogenesis under multiple aspects
    C Locht
    INSERM U447, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019, Lille Cedex, France
    Curr Opin Microbiol 4:82-9. 2001
    ..They are regulated by iron, although in one case, the production of a siderophore receptor could be linked to the BvgAS regulon...
  8. doi request reprint [New antituberculosis vaccines]
    C Locht
    Inserm 1019, centre d infection et immunité de Lille, CNRS UMR9204, Universite Lille Nord de France, Institut Pasteur de Lille, 1, rue du Professeur Calmette, 59019 Lille Cedex, France
    Arch Pediatr 18:1023-7. 2011
    ..Although still far from human applications, it is hoped that these strategies will ultimately help to reduce the enormous burden that is caused by tuberculosis...
  9. ncbi request reprint Molecular aspects of Bordetella pertussis pathogenesis
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM, Institut Pasteur de Lille, France
    Int Microbiol 2:137-44. 1999
    ..pertussis strains to be used as recombinant live bacterial vaccine vectors for homologous and heterologous protection...
  10. ncbi request reprint How a different look at latency can help to develop novel diagnostics and vaccines against tuberculosis
    Camille Locht
    INSERM U629, Lille, France
    Expert Opin Biol Ther 7:1665-77. 2007
    ..Potential implications for new diagnostic and vaccine design are discussed...
  11. ncbi request reprint Heparin-binding hemagglutinin, from an extrapulmonary dissemination factor to a powerful diagnostic and protective antigen against tuberculosis
    Camille Locht
    INSERM U629, Institut Pasteur de Lille, France
    Tuberculosis (Edinb) 86:303-9. 2006
    ..This latter activity is most likely perforin mediated. Together, these observations strongly support the potential of methylated HBHA as an important component in future, acellular vaccines against tuberculosis...
  12. doi request reprint A common vaccination strategy to solve unsolved problems of tuberculosis and pertussis?
    Camille Locht
    INSERM U629, Lille, France
    Microbes Infect 10:1051-6. 2008
    ....
  13. ncbi request reprint Bordetella pertussis from functional genomics to intranasal vaccination
    Camille Locht
    INSERM U447, Institut Pasteur de Lille, Lille, France
    Int J Med Microbiol 293:583-8. 2004
    ..Such strains may also serve as vaccine carriers for heterologous antigens, in order to vaccinate against several different pathogens simultaneously...
  14. ncbi request reprint Novel topological features of FhaC, the outer membrane transporter involved in the secretion of the Bordetella pertussis filamentous hemagglutinin
    S Guédin
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Biol Chem 275:30202-10. 2000
    ..The surface exposure of the N terminus and the presence of an odd number of beta-strands are novel features for beta-barrel outer membrane proteins...
  15. pmc Induction of mucosal immune responses against a heterologous antigen fused to filamentous hemagglutinin after intranasal immunization with recombinant Bordetella pertussis
    G Renauld-Mongenie
    Centre d Immunologie et de Biologie Parasitaire Institut National de la Santé et de la Recherche Médicale U167, Lille, France
    Proc Natl Acad Sci U S A 93:7944-9. 1996
    ....
  16. pmc Bordetella pertussis filamentous hemagglutinin enhances the immunogenicity of liposome-delivered antigen administered intranasally
    O Poulain-Godefroy
    INSERM U167, Institut Pasteur de Lille, France
    Infect Immun 66:1764-7. 1998
    ..These results show a promising potential for FHA to enhance the immunogenicity of mucosally administered antigens incorporated into liposomes...
  17. ncbi request reprint Tetanus toxin fragment C-specific priming by intranasal infection with recombinant Bordetella pertussis
    N Reveneau
    INSERM U447, Institut Pasteur de Lille, rue du Pr. Calmette, F-59019 Lille, France
    Vaccine 20:926-33. 2001
    ..pertussis for the development of combined childhood vaccines...
  18. ncbi request reprint The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination
    K Pethe
    Unité INSERM U447, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019 Lille cedex, France
    Nature 412:190-4. 2001
    ..They also suggest that antibody responses to HBHA may add to immune protection against tuberculosis...
  19. pmc Bordetella pertussis TonB, a Bvg-independent virulence determinant
    E Pradel
    INSERM U447, Institut Pasteur de Lille, 59019 Lille Cedex, France
    Infect Immun 68:1919-27. 2000
    ..Nevertheless, in vivo in the mouse respiratory infection model, the colonization ability of the mutant was reduced compared to the parental strain...
  20. pmc Molecular characterization of the mycobacterial heparin-binding hemagglutinin, a mycobacterial adhesin
    F D Menozzi
    Laboratoire de Microbiologie Génétique et Moléculaire, Institut National de la Santé et de la Recherche Médicale U447, Institut Pasteur de Lille, 1 rue Calmette, F 59019 Lille cedex, France
    Proc Natl Acad Sci U S A 95:12625-30. 1998
    ..Finally, the absence of a signal peptide in the coding region of HBHA raises the possibility that this protein is not secreted via the general secretion pathway...
  21. pmc Systemic and mucosal immune responses after intranasal administration of recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing glutathione S-transferase from Schistosoma haematobium
    L Kremer
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex, France
    Infect Immun 66:5669-76. 1998
    ..This difference was not observed after intraperitoneal administration...
  22. ncbi request reprint Variable human minisatellite-like regions in the Mycobacterium tuberculosis genome
    P Supply
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, France
    Mol Microbiol 36:762-71. 2000
    ..Of the 12 variable loci, only one was found to vary among genealogically distant BCG substrains, suggesting that these interspersed bacterial minisatellite-like structures evolve slowly in mycobacterial populations...
  23. pmc Identification and purification of transferrin- and lactoferrin-binding proteins of Bordetella pertussis and Bordetella bronchiseptica
    F D Menozzi
    , Institut Pasteur de Lille, France
    Infect Immun 59:3982-8. 1991
    ..Growth of the organisms under modulating conditions showed that the production of these IRPs is not under the genetic transcriptional control of vir or bvg, the general virulence regulon in Bordetella spp...
  24. ncbi request reprint Biochemical characterization of acyl carrier protein (AcpM) and malonyl-CoA:AcpM transacylase (mtFabD), two major components of Mycobacterium tuberculosis fatty acid synthase II
    L Kremer
    INSERM U447, Institut Pasteur de Lille, 1 rue du Pr. Calmette, BP245-59019 Lille Cedex, France
    J Biol Chem 276:27967-74. 2001
    ..tuberculosis. Since a specific type of FabD is found in mycobacterial species, it represents an attractive new drug target waiting to be exploited...
  25. pmc Production of Neisseria meningitidis transferrin-binding protein B by recombinant Bordetella pertussis
    I Coppens
    , INSERM U447, Institut Pasteur de Lille, F 59019 Lille, France
    Infect Immun 69:5440-6. 2001
    ..meningitidis. These observations indicate that recombinant B. pertussis may be a promising vector for the development of a mucosal vaccine against serogroup B meningococci...
  26. pmc Subtilisin-like autotransporter serves as maturation protease in a bacterial secretion pathway
    L Coutte
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    EMBO J 20:5040-8. 2001
    ..SphB1 is the first described subtilisin-like protein that serves as a specialized maturation protease in a secretion pathway of Gram-negative bacteria. This is reminiscent of pro-protein convertases of eukaryotic cells...
  27. pmc Role of ADP-ribosyltransferase activity of pertussis toxin in toxin-adhesin redundancy with filamentous hemagglutinin during Bordetella pertussis infection
    S Alonso
    INSERM U447, IBL, Institut Pasteur de Lille, F-59019 Lille, France
    Infect Immun 69:6038-43. 2001
    ..This may lead to damage of the pulmonary epithelium, allowing the bacteria to colonize even in the absence of FHA...
  28. ncbi request reprint Immunostimulatory effect of IL-18-encoding plasmid in DNA vaccination against murine Schistosoma mansoni infection
    L Dupre
    Laboratoire des Relations Hôtes Parasite et Stratégies Vaccinales, INSERM U 167, Institut Pasteur de Lille, F 59019 Cedex, Lille, France
    Vaccine 19:1373-80. 2001
    ..Therefore, this study shows that codelivery of an IL-18-encoding plasmid with an antigen-encoding plasmid can stimulate specific cellular responses and induce protective effects against S. mansoni infection...
  29. pmc Expression of the putative siderophore receptor gene bfrZ is controlled by the extracytoplasmic-function sigma factor BupI in Bordetella bronchiseptica
    E Pradel
    INSERM U447, Institut Pasteur de Lille, 59019 Lille Cedex, France
    J Bacteriol 183:2910-7. 2001
    ..By analogy with the FecI-FecR-FecA and PupI-PupR-PupB systems, our data suggest that bfrZ expression is inducible by binding of the cognate ligand to BfrZ and transduction of a signal through the envelope...
  30. pmc Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis
    Mara Cardoso Oelemann
    INSERM, U629, Institut Pasteur de Lille, Lille, France
    J Clin Microbiol 45:691-7. 2007
    ..tuberculosis transmission...
  31. ncbi request reprint Molecular characterization of Bordetella bronchiseptica filamentous haemagglutinin and its secretion machinery
    F Jacob-Dubuisson
    INSERM U447, Institut de Biologie de Lille, France
    Microbiology 146:1211-21. 2000
    ..Characterization of FHA expression and function may provide clues as to the basis of host species tropism, tissue localization and receptor recognition...
  32. doi request reprint Role of DegP for two-partner secretion in Bordetella
    C Baud
    INSERM U629, Lille, France
    Mol Microbiol 74:315-29. 2009
    ..In vitro, DegP binds to non-native FHA with high affinity. We propose that DegP chaperones the extended FHA polypeptide in the periplasm and is thus involved in the TPS pathway...
  33. ncbi request reprint Genomics of Bordetella pertussis toxins
    R Antoine
    INSERM U447, Institut Pasteur de Lille, France
    Int J Med Microbiol 290:301-5. 2000
    ..pertussis chromosome by homologous recombination. The reporter gene present in this vector allowed us to show that at least some of the bug genes are expressed...
  34. ncbi request reprint Channel formation by FhaC, the outer membrane protein involved in the secretion of the Bordetella pertussis filamentous hemagglutinin
    F Jacob-Dubuisson
    INSERM U447, Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Biol Chem 274:37731-5. 1999
    ..We thus propose that FhaC forms a specific beta-barrel channel in the outer membrane for the outward translocation of FHA...
  35. pmc Common accessory genes for the Bordetella pertussis filamentous hemagglutinin and fimbriae share sequence similarities with the papC and papD gene families
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF91 09, Lille, France
    EMBO J 11:3175-83. 1992
    ..Deletion analyses suggest that fhaB and the downstream genes can be transcribed as a polycistronic operon, and primer extension analysis revealed the presence of a second promoter between fhaB and fhaD...
  36. pmc Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units
    P Supply
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex, France
    J Clin Microbiol 39:3563-71. 2001
    ..tuberculosis MIRU-VNTR genotypes via the Internet. This opens the way for global epidemiological surveillance of tuberculosis and should lead to novel insights into the evolutionary and population genetics of this major pathogen...
  37. doi request reprint The coiled-coil N-terminal domain of the heparin-binding haemagglutinin is required for the humoral and cellular immune responses in mice
    Gloria G Guerrero
    INSERM, U629, Lille, France
    Mol Immunol 46:116-24. 2008
    ..Altogether we provide evidence that the coiled-coil N-terminal domain is required for HBHA immunogenicity in vivo...
  38. ncbi request reprint Two-partner secretion in Gram-negative bacteria: a thrifty, specific pathway for large virulence proteins
    F Jacob-Dubuisson
    INSERM U447, IBL, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    Mol Microbiol 40:306-13. 2001
    ..Several adhesins also undergo extensive proteolytic processing upon secretion. The genes of many new TpsAs and TpsBs are found in recently sequenced genomes, suggesting that the TPS pathway is widespread...
  39. ncbi request reprint Glutathione S-transferases of 28kDa as major vaccine candidates against schistosomiasis
    G Riveau
    INSERM U167, Institut Pasteur de Lille, France
    Mem Inst Oswaldo Cruz 93:87-94. 1998
    ..Recent data on acquired immunity directed to 28GST in infected human populations, and new development to draw adapted vaccine formulations, are presented...
  40. ncbi request reprint Identification of novel intergenic repetitive units in a mycobacterial two-component system operon
    P Supply
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U447, Institut Pasteur de Lille, France
    Mol Microbiol 26:991-1003. 1997
    ..Analyses of the sequences at the insertion sites suggest that MIRUs disseminate by transposition into DTGA sites involved in translational coupling in polycistronic operons...
  41. ncbi request reprint Structure-based mechanism of ligand binding for periplasmic solute-binding protein of the Bug family
    J Herrou
    INSERM U629, 1 rue du Pr Calmette, 59019 Lille Cedex, France
    J Mol Biol 373:954-64. 2007
    ..Stabilization of the closed conformation requires proper interactions to be established with domain 2, and thus domain 2 discriminates between productively and non-productively bound ligands...
  42. ncbi request reprint Adjuvant activity of free Bordetella pertussis filamentous haemagglutinin delivered by mucosal routes
    O Poulain-Godefroy
    INSERM U547, Institut Pasteur de Lille, Lille Cedex, France
    Scand J Immunol 58:503-10. 2003
    ....
  43. pmc Recombinant HBHA boosting effect on BCG-induced immunity against Mycobacterium tuberculosis infection
    G G Guerrero
    Inserm U1019, Lille, France
    Clin Dev Immunol 2011:730702. 2011
    ..This report thus provides evidence that rHBHA may be considered as a booster vaccine against disseminated tuberculosis...
  44. ncbi request reprint Nasal vaccination using live bacterial vectors
    N Mielcarek
    INSERM U447, IBL, Institut Pasteur of Lille, 1 rue du Pr. Calmette, 59019, Lille, France
    Adv Drug Deliv Rev 51:55-69. 2001
    ..The strategies of expression of the heterologous antigens, the invasiveness and the immunogenicity of the recombinant bacteria are discussed...
  45. pmc Stability of variable-number tandem repeats of mycobacterial interspersed repetitive units from 12 loci in serial isolates of Mycobacterium tuberculosis
    Evgueni Savine
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex, France
    J Clin Microbiol 40:4561-6. 2002
    ..Moreover, they support MIRU VNTR genotyping as a powerful first-line method followed by subtyping by IS6110 RFLP to define ongoing transmission clusters...
  46. pmc Identification of a heparin-binding hemagglutinin present in mycobacteria
    F D Menozzi
    Laboratoire de Microbiologie Genetique et Moléculare, Institut National de la Santé et de la Recherche Médicale U447, Institut Pasteur de Lille, France
    J Exp Med 184:993-1001. 1996
    ..Identification of putative mycobacterial adhesins, such as the one described in this report, may provide the basis for the development of new therapeutic and prophylactic strategies against mycobacterial diseases...
  47. ncbi request reprint [Cellular and acellular anti-pertussis vaccines]
    C Locht
    INSERM U447, Institut Pasteur de Lille, 1 rue du Prof. Calmette, F59019 Lille Cedex
    Ann Pharm Fr 59:198-205. 2001
    ..In addition, the construction of recombinant attenuated strains would perhaps simultaneously protect against whooping cough and other infectious or parasitic diseases...
  48. pmc High-resolution minisatellite-based typing as a portable approach to global analysis of Mycobacterium tuberculosis molecular epidemiology
    E Mazars
    , , U447, Institut Pasteur de Lille, rue du Prof. Calmette, F-59019 Lille Cedex, France
    Proc Natl Acad Sci U S A 98:1901-6. 2001
    ..This typing method opens the way to the construction of digital global databases for molecular epidemiology studies of M. tuberculosis...
  49. ncbi request reprint A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit
    C Locht
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM CJF 9109, Institut Pasteur de Lille, France
    Biochimie 77:333-40. 1995
    ..His-35 could increase the nucleophilicity of the cysteine in the G protein or the water molecule to attack the weakened N-glycosidic bond of NAD+ and yield the products of the reaction...
  50. pmc Role of adhesin release for mucosal colonization by a bacterial pathogen
    Loic Coutte
    INSERM U447, Institut National de Sante et la Recherche Medical Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Exp Med 197:735-42. 2003
    ..These results indicate that the release of FHA is important for colonization, as it may facilitate the dispersal of bacteria from microcolonies and the binding to new sites in the respiratory tract...
  51. pmc Molecular evolution of the two-component system BvgAS involved in virulence regulation in Bordetella
    Julien Herrou
    Institut National de la Santé Et de la Recherche Médicale U629, Lille, France
    PLoS ONE 4:e6996. 2009
    ..The repertoire of BvgS sequences will pave the way for functional analyses of this prototypic system...
  52. doi request reprint Long-term immunity against pertussis induced by a single nasal administration of live attenuated B. pertussis BPZE1
    Pascal Feunou Feunou
    Institut National de la Santé et de la Recherche Médicale U1019, F 59019, Lille, France
    Vaccine 28:7047-53. 2010
    ..These data highlight the potential of the live attenuated BPZE1 candidate vaccine as part of a strategy to solve the problem of waning protective immunity against B. pertussis observed with the current aPV vaccines...
  53. ncbi request reprint Differential modulation of Bordetella pertussis virulence genes as evidenced by DNA microarray analysis
    D Hot
    Laboratoire des Biopuces, Institut Pasteur de Lille, 1 rue du Prof Calmette, 59019 Lille, France
    Mol Genet Genomics 269:475-86. 2003
    ..Furthermore, surprisingly, in the strain analysed here, which produces only type 2 fimbriae, the fim3 gene was identified as a vrg, while fim2 was confirmed to be a vag...
  54. ncbi request reprint Interaction of Bordetella pertussis with mast cells, modulation of cytokine secretion by pertussis toxin
    N Mielcarek
    Department of Medical Microbiology and Immunology, University of Goteborg, Sweden
    Cell Microbiol 3:181-8. 2001
    ..pertussis-infected mice. These data suggest that mast cells may play a role in the induction of immune responses against B. pertussis through the release of cytokines, especially TNF-alpha...
  55. pmc Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis
    Philip Supply
    INSERM U629, Institut Pasteur de Lille, 1, Rue du Prof Calmette, F 59019 Lille cedex, France
    J Clin Microbiol 44:4498-510. 2006
    ..This 15-locus system is therefore proposed as the new standard for routine epidemiological discrimination of M. tuberculosis isolates and the 24-locus system as a high-resolution tool for phylogenetic studies...
  56. pmc Live attenuated B. pertussis as a single-dose nasal vaccine against whooping cough
    Nathalie Mielcarek
    1INSERM U629, Lille, France
    PLoS Pathog 2:e65. 2006
    ..BPZE1 is thus an attractive vaccine candidate to protect against whooping cough by nasal, needle-free administration early in life, possibly at birth...
  57. doi request reprint The peptidyl-prolyl isomerase and chaperone Par27 of Bordetella pertussis as the prototype for a new group of parvulins
    Hélène Hodak
    INSERM U629, Lille, France
    J Mol Biol 376:414-26. 2008
    ..Par27 displays both PPIase and chaperone activities in vitro. In vivo, Par27 might function as a general periplasmic chaperone in B. pertussis...
  58. ncbi request reprint Interaction of human Tamm-Horsfall glycoprotein with Bordetella pertussis toxin
    Franco D Menozzi
    Institut Pasteur de Lille, INSERM U447, Mécanismes Moléculaires de la Pathogénie Microbienne, 1 rue du Professeur Calmette, 59019 Lille Cedex, France
    Microbiology 148:1193-201. 2002
    ..These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces...
  59. ncbi request reprint Enhanced bacterial virulence through exploitation of host glycosaminoglycans
    Franco D Menozzi
    INSERM IPL U 447, Institut Pasteur de Lille, France
    Mol Microbiol 43:1379-86. 2002
    ..The exploitation of PGs by pathogenic bacteria is thus a multifaceted mechanistic process directly related to the potential virulence of a number of microorganisms...
  60. pmc Eighty-kilodalton N-terminal moiety of Bordetella pertussis filamentous hemagglutinin: adherence, immunogenicity, and protective role
    Sylvie Alonso
    INSERM U447, IBL, Institut Pasteur de Lille, France
    Infect Immun 70:4142-7. 2002
    ..Compared to FHA, Fha44 is much smaller and much more soluble and is therefore easier to purify and to store. These advantages may perhaps warrant considering Fha44 for inclusion in acellular pertussis vaccines...
  61. ncbi request reprint In vivo interaction between the polyprenol phosphate mannose synthase Ppm1 and the integral membrane protein Ppm2 from Mycobacterium smegmatis revealed by a bacterial two-hybrid system
    Alain R Baulard
    INSERM U447, Institut Pasteur de Lille Institut de Biologie de Lille, France
    J Biol Chem 278:2242-8. 2003
    ..This interaction is reminiscent of that of mammalian Dpm1 with Dpm3 and mimics the structure of MtPpm1 as demonstrated by the capacity of the two domains of MtPpm1 to spontaneously interact when co-expressed in E. coli...
  62. ncbi request reprint Linkage disequilibrium between minisatellite loci supports clonal evolution of Mycobacterium tuberculosis in a high tuberculosis incidence area
    Philip Supply
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, Cedex, France
    Mol Microbiol 47:529-38. 2003
    ..This linkage disequilibrium was also evident when the MIRU-VNTR types were compared with the IS6110 restriction fragment length polymorphism types. These results support a predominant clonal evolution of M. tuberculosis...
  63. pmc Dose response of attenuated Bordetella pertussis BPZE1-induced protection in mice
    Nathalie Mielcarek
    Inserm U1019, Institut Pasteur de Lille, Lille Cedex, France
    Clin Vaccine Immunol 17:317-24. 2010
    ..pertussis antigens. These two parameters may perhaps be used as readouts in clinical trials in humans that are currently being planned...
  64. ncbi request reprint Recombinant Mycobacterium bovis BCG producing IL-18 reduces IL-5 production and bronchoalveolar eosinophilia induced by an allergic reaction
    F Biet
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U629, Lille, France
    Allergy 60:1065-72. 2005
    ..The induction of IFN-gamma is mediated by interleukin (IL)-12 known to be secreted upon mycobacterial infections and can be enhanced by IL-18 acting in synergy with IL-12...
  65. ncbi request reprint Structure of the membrane protein FhaC: a member of the Omp85-TpsB transporter superfamily
    Bernard Clantin
    UMR8161 CNRS, Institut de Biologie de Lille, Universite de Lille 1, Universite de Lille 2, 1 rue du Prof Calmette, F 59021 Lille cedex, France
    Science 317:957-61. 2007
    ..General features of the mechanism obtained here are likely to apply throughout the superfamily...
  66. ncbi request reprint Attenuated Bordetella pertussis: new live vaccines for intranasal immunisation
    Nathalie Mielcarek
    INSERM U629, IBL, Institut Pasteur de Lille, 1, rue du Pr Calmette, 59019 Lille, France
    Vaccine 24:S2-54-5. 2006
    ..Moreover, live recombinant B. pertussis may be used as a vector for intranasal vaccination against a variety of diseases...
  67. doi request reprint Subcutaneous boosting with heparin binding haemagglutinin increases BCG-induced protection against tuberculosis
    Carine Rouanet
    INSERM U629, F 59019 Lille, France
    Microbes Infect 11:995-1001. 2009
    ..7log when compared to BCG alone. These results suggest an immunisation strategy where BCG is administered to neonates and is followed by subcutaneous HBHA boosting in young adults...
  68. ncbi request reprint The periplasmic binding protein of a tripartite tricarboxylate transporter is involved in signal transduction
    Rudy Antoine
    INSERM U629, Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    J Mol Biol 351:799-809. 2005
    ..Thus, BctC is part of the signalling cascade leading to upregulation of the transporter operon in the presence of its solute, a new function for periplasmic binding proteins of TT transporters...
  69. pmc Mycobacterial heparin-binding hemagglutinin and laminin-binding protein share antigenic methyllysines that confer resistance to proteolysis
    Kevin Pethe
    Institut National de la Santé et de la Recherche Médicale U447, Lille Cedex, France
    Proc Natl Acad Sci U S A 99:10759-64. 2002
    ..This hypothesis was confirmed by the fact that HBHA produced by recombinant M. smegmatis was also methylated. These results show that mycobacteria use enzymatic methylation of lysines to ensure greater stability of their adhesins...
  70. doi request reprint Genetic stability of the live attenuated Bordetella pertussis vaccine candidate BPZE1
    Pascal Feunou Feunou
    INSERM U629, Lille, France
    Vaccine 26:5722-7. 2008
    ....
  71. ncbi request reprint Intracellular autoregulation of the Mycobacterium tuberculosis PrrA response regulator
    Fanny Ewann
    INSERM U447, Institut Pasteur de Lille, 1 rue du Professeur Calmette, F 59019 Lille, France
    Microbiology 150:241-6. 2004
    ..These results indicate that the early intracellular induction of prrA depends on the autoregulation of this two-component system...
  72. ncbi request reprint Genetic exchange of the S2 and S3 subunits in pertussis toxin
    Dominique Raze
    INSERM U629, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France
    Mol Microbiol 60:1241-50. 2006
    ..These toxin analogues constitute thus interesting probes for the study of cellular functions, in particular immune cell functions, for which natural PTX has already shown its usefulness...
  73. ncbi request reprint Secretion signal of the filamentous haemagglutinin, a model two-partner secretion substrate
    Hélène Hodak
    INSERM U629, 1 rue Calmette, F 59019 Lille cedex, France
    Mol Microbiol 61:368-82. 2006
    ..The N-terminal domain of FhaC is involved in the FHA-FhaC interaction, in agreement with its proposed function and periplasmic localization...
  74. doi request reprint Bordetella pertussis filamentous hemagglutinin delivered by mucosal routes enhances immunoglobulin levels in serum and mucosal fluids
    Odile Poulain-Godefroy
    INSERM U547, Lille, Cedex, France
    FEMS Immunol Med Microbiol 54:129-36. 2008
    ..FHA induces Langerhans cell recruitment and MIP-3alpha mRNA expression within hours after administration. These observations shed a new light on the potential molecular mechanisms of FHA-induced adjuvanticity...
  75. ncbi request reprint Enzymatic methylation of the Mycobacterium tuberculosis heparin-binding haemagglutinin
    Hélène Host
    INSERM U629, Lille, France
    FEMS Microbiol Lett 268:144-50. 2007
    ..Using recombinant hybrid molecules as acceptor substrates, it was found that the N-terminal domain of HBHA is not required for recognition by the HBHA-methyltransferase(s), although it is required for in vivo methylation...
  76. ncbi request reprint Crystal structures of two Bordetella pertussis periplasmic receptors contribute to defining a novel pyroglutamic acid binding DctP subfamily
    Prakash Rucktooa
    UMR 8161 CNRS Institut de Biologie de Lille, Laboratoire de Cristallographie Macromoléculaire, Universite des Sciences et Technologies de Lille, Universite de Lille 2, Institut Pasteur de Lille IFR142, Lille Cedex, France
    J Mol Biol 370:93-106. 2007
    ....
  77. ncbi request reprint Surface anchoring of bacterial subtilisin important for maturation function
    Loic Coutte
    INSERM U44, Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue Calmette, 59019 Lille Cedex, France
    Mol Microbiol 49:529-39. 2003
    ..The tethered protease appears to be stabilized by unusual Gly- and Pro-rich motifs at the N-terminus of the protein. This represents a new mode of localization for a protease involved in protein secretion...
  78. ncbi request reprint Crystal structure of Bordetella pertussis BugD solute receptor unveils the basis of ligand binding in a new family of periplasmic binding proteins
    Isabelle Huvent
    CNRS UMR8117 Institut de Biologie de Lille, Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP245 59021 Lille cedex, France
    J Mol Biol 356:1014-26. 2006
    ..The present structure thus unveils the bases of ligand binding in this large family of periplasmic binding proteins, several hundred members of which have been identified in various bacterial species...
  79. ncbi request reprint Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens
    Franck Biet
    Laboratoire de Microbiologie Génétique et Moléculaire, Institut National de la Santé et de la Recherche Médicale U447, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59021 Lille, France
    J Mol Med (Berl) 80:147-62. 2002
    ..Since this cytokine may be particularly useful in immunoprophylactic and immunotherapeutic interventions in which the cellular response is most desirable, the potential therapeutic aspects of IL-18 is also discussed...
  80. ncbi request reprint Mycobacterium tuberculosis heparin-binding haemagglutinin adhesin (HBHA) triggers receptor-mediated transcytosis without altering the integrity of tight junctions
    Franco D Menozzi
    INSERM, U629, Mécanismes Moléculaires de la Pathogénie Microbienne, Institut Pasteur de Lille, 1, rue du Professeur Calmette, 59019 Lille Cedex, France
    Microbes Infect 8:1-9. 2006
    ..tuberculosis...
  81. pmc Mycobacterium bovis BCG producing interleukin-18 increases antigen-specific gamma interferon production in mice
    Franck Biet
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U447, Institut Pasteur de Lille, France
    Infect Immun 70:6549-57. 2002
    ..This may be particularly useful for immunotherapeutic or prophylactic interventions in which a Th1 response is most desirable...
  82. pmc Transient requirement of the PrrA-PrrB two-component system for early intracellular multiplication of Mycobacterium tuberculosis
    Fanny Ewann
    Laboratoire des Mécanismes Moléculaires de la Pathogenèse Bactérienne, INSERM U447, Institut Pasteur de Lille, F 59019 Lille cedex
    Infect Immun 70:2256-63. 2002
    ..tuberculosis can use genes temporarily required at different stages in the course of macrophage infection...
  83. pmc Overrepresentation of a gene family encoding extracytoplasmic solute receptors in Bordetella
    Rudy Antoine
    INSERM U447 CNRS UMR 8525, Institut de Biologie de Lille, Institut Pasteur de Lille, 59019 Lille Cedex, France
    J Bacteriol 185:1470-4. 2003
    ..pertussis, and one is involved in citrate uptake. The bug genes probably form an ancient family that has been subjected to a large expansion in a restricted phylogenic group...
  84. ncbi request reprint Comparison of the immune responses induced by local immunizations with recombinant Lactobacillus plantarum producing tetanus toxin fragment C in different cellular locations
    Nathalie Reveneau
    Laboratoire de Bactériologie des Ecosystèmes, INSERM IFR17, Institut Pasteur de Lille, 1 rue du Professeur Calmette, F 59019 Lille cedex, France
    Vaccine 20:1769-77. 2002
    ..While cell-surface presentation required lower antigen doses to be immunogenic, the highest IgG serum antibody titers were obtained with the strain producing large amounts of TTFC in the cytoplasm...
  85. pmc Production of nontypeable Haemophilus influenzae HtrA by recombinant Bordetella pertussis with the use of filamentous hemagglutinin as a carrier
    Sylvie Alonso
    INSERM U629, Institut Pasteur de Lille, 1 rue du Prof Calmette, F 59019 Lille, France
    Infect Immun 73:4295-301. 2005
    ..pertussis strain producing the Fha44-HtrA chimera colonized the mouse lungs more efficiently than the parental, Fha44-producing strain and gave rise to higher anti-FHA antibody titers than those induced by the parental strain...
  86. ncbi request reprint Inhibition of InhA activity, but not KasA activity, induces formation of a KasA-containing complex in mycobacteria
    Laurent Kremer
    Laboratoire des Mécanismes Moléculaires de la Pathogénie Microbienne, INSERM U447, Institut Pasteur de Lille IBL, Lille, France
    J Biol Chem 278:20547-54. 2003
    ..1) Only inhibition of InhA, but not KasA, induces the KasA-containing complex. 2) INH is not part of the complex. 3) INH does not target KasA, consistent with InhA being the primary target of INH...
  87. doi request reprint Synthetic EthR inhibitors boost antituberculous activity of ethionamide
    Nicolas Willand
    Institut National de la Sante et de la Recherche Medicale, Lille, France
    Nat Med 15:537-44. 2009
    ..This provides proof of concept that inhibiting EthR improves the therapeutic index of thiocarbamide derivatives, which should prompt reconsideration of their use as first-line drugs...
  88. pmc The crystal structure of filamentous hemagglutinin secretion domain and its implications for the two-partner secretion pathway
    Bernard Clantin
    Institut Fédératif de Recherche du Centre National de la Recherche Scientifique 3, Lille Cedex, France
    Proc Natl Acad Sci U S A 101:6194-9. 2004
    ..This structure is representative of the TPS domains found so far in >100 secreted proteins from pathogenic bacteria. It also provides a mechanistic insight into how protein folding may be linked to secretion in the TPS pathway...
  89. pmc Immune response induced by recombinant Mycobacterium bovis BCG producing the cholera toxin B subunit
    Franck Biet
    Laboratoire de Microbiologie Génétique et Moléculaire, INSERM U447, IBL, Institut Pasteur de Lille, F 59019 Lille, France
    Infect Immun 71:2933-7. 2003
    ..These results suggest that the CTB-producing BCG may be a powerful adjuvant to be considered for future mucosal vaccine development...
  90. ncbi request reprint Mycobacterium smegmatis produces an HBHA homologue which is not involved in epithelial adherence
    Franck Biet
    UR1282, Infectiologie Animale, Santé Publique IASP 311, INRA Centre de Tours, F 37380 Nouzilly France
    Microbes Infect 9:175-82. 2007
    ..We therefore conclude that TB-HBHA may have evolved as an adhesin in pathogenic mycobacteria from a homolog that serves a different function in a saprophytic mycobacterium...
  91. pmc Immunogenicity and protective efficacy of neonatal vaccination against Bordetella pertussis in a murine model: evidence for early control of pertussis
    Caroline Roduit
    World Health Organization Collaborating Center for Vaccinology and Neonatal Immunology, Department of Pathology and Pediatrics, University of Geneva, Geneva, Switzerland
    Infect Immun 70:3521-8. 2002
    ..pertussis challenge. Thus, neonatal priming with antipertussis vaccines should be considered to reduce the window of vulnerability to pertussis at the time of its greatest severity...
  92. ncbi request reprint Genotyping of the Mycobacterium tuberculosis complex using MIRUs: association with VNTR and spoligotyping for molecular epidemiology and evolutionary genetics
    Christophe Sola
    Unite de la Tuberculose et des Mycobacteries, Institut Pasteur de Guadeloupe, Morne Joliviere, BP 484, F 97165 Pointe à Pitre Cédex, Guadeloupe, France
    Infect Genet Evol 3:125-33. 2003
    ..tuberculosis phylogeny reconstruction. We also conclude that MIRU typing is a very promising method that may be used in a "two PCR-based" genotyping strategy, in conjunction to conventional epidemiological investigations...
  93. ncbi request reprint Bordetella pertussis infection in 2-month-old infants promotes type 1 T cell responses
    Francoise Mascart
    Laboratory of Immunology, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
    J Immunol 170:1504-9. 2003
    ..We conclude that the immune system of 2-mo-old infants is developmentally mature enough to develop Th1 responses in vivo upon infection by B. pertussis or vaccination with whole-cell pertussis vaccines...
  94. ncbi request reprint Differential T and B cell responses against Mycobacterium tuberculosis heparin-binding hemagglutinin adhesin in infected healthy individuals and patients with tuberculosis
    Chantal Masungi
    Laboratory of Immunology, Erasme Hospital, Brussels, Belgium
    J Infect Dis 185:513-20. 2002
    ..These observations indicate that HBHA is recognized differently by the immune systems of patients with tuberculosis and infected healthy individuals and might provide a marker for protection against tuberculosis...
  95. pmc Single-molecule force spectroscopy of mycobacterial adhesin-adhesin interactions
    Claire Verbelen
    Unite de Chimie des Interfaces, Universite Catholique de Louvain, Croix du Sud 2 18, B 1348 Louvain la Neuve, Belgium
    J Bacteriol 189:8801-6. 2007
    ..Together with the fact that HBHA mediates bacterial aggregation, these observations suggest that the single homophilic HBHA interactions measured here reflect the formation of multimers that may promote mycobacterial aggregation...
  96. pmc Heparin-binding-hemagglutinin-induced IFN-gamma release as a diagnostic tool for latent tuberculosis
    Jean Michel Hougardy
    Laboratory of Vaccinology and Mucosal Immunity, Hopital Erasme, Universite Libre de Bruxelles, Brussels, Belgium
    PLoS ONE 2:e926. 2007
    ..We have therefore evaluated the potential of the novel antigen heparin-binding hemagglutinin (HBHA) for in vitro detection of LTBI...
  97. pmc Use of mycobacterial interspersed repetitive unit-variable-number tandem repeat typing to examine genetic diversity of Mycobacterium tuberculosis in Singapore
    Yong Jiang Sun
    Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Republic of Singapore
    J Clin Microbiol 42:1986-93. 2004
    ..These results indicate that MIRU-VNTR typing can be a useful first-line tool for studying the genetic diversity of M. tuberculosis isolates in a large urban setting such as Singapore...
  98. ncbi request reprint In vitro expansion of CD4+CD25highFOXP3+CD127low/- regulatory T cells from peripheral blood lymphocytes of healthy Mycobacterium tuberculosis-infected humans
    Jean Michel Hougardy
    Laboratory of Vaccinology and Mucosal Immunity, Universite Libre de Bruxelles, Belgium
    Microbes Infect 9:1325-32. 2007
    ....
  99. ncbi request reprint Antibody response in pulmonary tuberculosis against recombinant 27kDa (MPT51, Rv3803c) protein of Mycobacterium tuberculosis
    Ramalingam Bethunaickan
    Department of Immunology, Tuberculosis Research Centre ICMR, Chetput, Chennai, India
    Scand J Infect Dis 39:867-74. 2007
    ..Measuring the IgG+IgA combination positivities of the CIC-bound antibodies gave a poor sensitivity of 21%, 18% and 10%, respectively. The specificity of the assay by these combinations was maintained at 94%...
  100. pmc The mycobacterial heparin-binding hemagglutinin is a protective antigen in the mouse aerosol challenge model of tuberculosis
    Marcela Parra
    Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, Food and Drug Administration, Bldg 29, Rm 503, HFM 431, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Infect Immun 72:6799-805. 2004
    ..tuberculosis are critical for generating effective host immune responses. The vaccine studies described here demonstrate that HBHA is a promising new vaccine candidate for tuberculosis...
  101. ncbi request reprint Channel properties of TpsB transporter FhaC point to two functional domains with a C-terminal protein-conducting pore
    Albano C Meli
    UMR 5048 CNRS, U554 INSERM, Centre de Biochimie Structurale, 29 rue de Navacelles, 34090 Montpellier Cedex, France
    J Biol Chem 281:158-66. 2006
    ..The N-terminal 200 residues of FhaC constitute a functionally distinct domain that modulates the pore properties and may participate in FHA recognition...