Philippe Langella

Summary

Affiliation: Institut National de la Recherche Agronomique
Country: France

Publications

  1. pmc Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii influence the production of mucus glycans and the development of goblet cells in the colonic epithelium of a gnotobiotic model rodent
    Laura Wrzosek
    INRA, UMR 1319 Micalis, AgroParisTech, Domaine de Vilvert, Jouy en Josas, 78350, France
    BMC Biol 11:61. 2013
  2. pmc Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
    Luis G Bermúdez-Humarán
    INRA, UMR1319 MICALIS, Commensal and Probiotics Host Interactions Laboratory, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microb Cell Fact 10:S4. 2011
  3. pmc Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium
    Naima G Cortes Perez
    , , Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France
    BMC Res Notes 2:167. 2009
  4. doi request reprint Primocolonization is associated with colonic epithelial maturation during conventionalization
    Julie Tomas
    Commensal and Probiotics Host Interactions Laboratory, Institut National de Recherche Agronomique, Jouy en Josas, France
    FASEB J 27:645-55. 2013
  5. pmc Carbohydrate metabolism is essential for the colonization of Streptococcus thermophilus in the digestive tract of gnotobiotic rats
    Muriel Thomas
    Commensal and Probiotics Host Interactions Laboratory, INRA, UMR1319 MICALIS, Jouy en Josas, France
    PLoS ONE 6:e28789. 2011
  6. pmc Impact of the metabolic activity of Streptococcus thermophilus on the colon epithelium of gnotobiotic rats
    Françoise Rul
    INRA, MICALIS UMR 1319, Domaine de Vilvert, F 78352 Jouy en Josas, France
    J Biol Chem 286:10288-96. 2011
  7. ncbi request reprint Influence of the route of immunization and the nature of the bacterial vector on immunogenicity of mucosal vaccines based on lactic acid bacteria
    Naima G Cortes-Perez
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Vaccine 25:6581-8. 2007
  8. ncbi request reprint A novel mucosal vaccine based on live Lactococci expressing E7 antigen and IL-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors
    Luis G Bermúdez-Humarán
    Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France
    J Immunol 175:7297-302. 2005
  9. doi request reprint Perspectives for the development of human papillomavirus vaccines and immunotherapy
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Expert Rev Vaccines 9:35-44. 2010
  10. ncbi request reprint Current prophylactic and therapeutic uses of a recombinant Lactococcus lactis strain secreting biologically active interleukin-12
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Jouy en Josas, France
    J Mol Microbiol Biotechnol 14:80-9. 2008

Collaborators

  • Claire Cherbuy
  • Muriel Thomas
  • Luis G Bermúdez-Humarán
  • Françoise Rul
  • Karine Adel-Patient
  • Nathalie Vergnolle
  • Lothar Steidler
  • Rebeca Martín
  • Catherine Robbe-Masselot
  • Olivier Berteau
  • Jerry M Wells
  • Corine Sandstrom
  • Corinne Grangette
  • Jean Fioramonti
  • Ronan Le Goffic
  • Laurent Ferrier
  • Djamel Drider
  • Vasco Azevedo
  • Jean Jacques Gratadoux
  • Naima G Cortes-Perez
  • Gerard Corthier
  • Julie Tomas
  • François Lefèvre
  • Jean Marc Chatel
  • Alexandra Gruss
  • Laura Wrzosek
  • Valeria Dellaretti Guimarães
  • Tatiana Rochat
  • Sébastien Blugeon
  • Laurie Watterlot
  • Silvia Innocentin
  • Anderson Miyoshi
  • Noura Kechaou
  • Stephan Bouet
  • Marie Louise Noordine
  • Marcela de Azevedo
  • Stéphane Pierre
  • Alain Guillot
  • Elvis Bernard
  • Marie Pierre Chapot-Chartier
  • Chantal Bridonneau
  • Fabrizio Chiaramonte
  • Pascale Kharrat
  • Naima G Cortes Perez
  • Luciana A Ribeiro
  • Harry Sokol
  • Jean Michel Wal
  • Sebastien Nouaille
  • Yves Le Loir
  • Sylvie Miquel
  • Christophe Chevalier
  • Edith Honvo-Houeto
  • Camille Mayeur
  • Stéphanie Courau
  • Marie Joncquel Chevalier-Curt
  • Pascal Molimard
  • Catherine Philippe
  • Véronique Robert
  • Nassim Bouznad
  • Nicolas Bertho
  • Florian Chain
  • Jurgen Karczewski
  • Alhosna Benjdia
  • Pascal Hols
  • Pascal Courtin
  • Thomas Rolain
  • Marie Christine Champomier-Vergès
  • Monique Zagorec
  • Patricia Anglade
  • Maria Jose Flores
  • Fabienne Baraige
  • Mickael Meyrand
  • Nicolas Gaide
  • Sandrine Ah-Leung
  • Valeria Guimaraes
  • Hervé M Blottiere
  • Philippe Pochart
  • Ginette Thomas
  • Philippe Seksik
  • Joel Dore
  • Philippe Marteau
  • Nadia Vasquez
  • Omar Lakhdari
  • Germain Trugnan
  • Jean Pierre Furet
  • Benedicte Pigneur
  • Emmanuelle Maguin
  • Maarten van de Guchte
  • Reyes S Tamez-Guerra
  • María Elena Nader-Macías

Detail Information

Publications36

  1. pmc Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii influence the production of mucus glycans and the development of goblet cells in the colonic epithelium of a gnotobiotic model rodent
    Laura Wrzosek
    INRA, UMR 1319 Micalis, AgroParisTech, Domaine de Vilvert, Jouy en Josas, 78350, France
    BMC Biol 11:61. 2013
    ..thetaiotaomicron affected goblet cell differentiation, mucin synthesis and glycosylation in the colonic epithelium. We then investigated how F. prausnitzii influenced the colonic epithelial responses to B. thetaiotaomicron...
  2. pmc Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
    Luis G Bermúdez-Humarán
    INRA, UMR1319 MICALIS, Commensal and Probiotics Host Interactions Laboratory, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microb Cell Fact 10:S4. 2011
    ....
  3. pmc Heterologous production of human papillomavirus type-16 L1 protein by a lactic acid bacterium
    Naima G Cortes Perez
    , , Institut National de la Recherche Agronomique, 78352 Jouy en Josas, France
    BMC Res Notes 2:167. 2009
    ..lactis, for the production of L1-based VLPs and open the possibility for the development of a new safe mucosal vector for HPV-16 prophylactic vaccination...
  4. doi request reprint Primocolonization is associated with colonic epithelial maturation during conventionalization
    Julie Tomas
    Commensal and Probiotics Host Interactions Laboratory, Institut National de Recherche Agronomique, Jouy en Josas, France
    FASEB J 27:645-55. 2013
    ..The colon matured in parallel with the evolution of the microbiota composition, and we therefore suggest a link between intestinal events regulating homeostasis of the colon and modulation of microbial composition...
  5. pmc Carbohydrate metabolism is essential for the colonization of Streptococcus thermophilus in the digestive tract of gnotobiotic rats
    Muriel Thomas
    Commensal and Probiotics Host Interactions Laboratory, INRA, UMR1319 MICALIS, Jouy en Josas, France
    PLoS ONE 6:e28789. 2011
    ..thermophilus in the GIT. Our results are in accord with the rationale that nutritional allegation via consumption of yogurt alleviates the symptoms of lactose intolerance...
  6. pmc Impact of the metabolic activity of Streptococcus thermophilus on the colon epithelium of gnotobiotic rats
    Françoise Rul
    INRA, MICALIS UMR 1319, Domaine de Vilvert, F 78352 Jouy en Josas, France
    J Biol Chem 286:10288-96. 2011
    ..thermophilus and modulating the colon epithelium...
  7. ncbi request reprint Influence of the route of immunization and the nature of the bacterial vector on immunogenicity of mucosal vaccines based on lactic acid bacteria
    Naima G Cortes-Perez
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Vaccine 25:6581-8. 2007
    ..plantarum play an important role in the immunogenicity of the expressed antigen...
  8. ncbi request reprint A novel mucosal vaccine based on live Lactococci expressing E7 antigen and IL-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors
    Luis G Bermúdez-Humarán
    Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France
    J Immunol 175:7297-302. 2005
    ..These preclinical results suggest the feasibility of the low-cost mucosal vaccination and/or immunotherapy strategies against HPV-related cervical cancer in humans...
  9. doi request reprint Perspectives for the development of human papillomavirus vaccines and immunotherapy
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Expert Rev Vaccines 9:35-44. 2010
    ..This review discusses the actual perspectives on both HPV vaccines and immunotherapy worldwide. In addition, some of the perspectives in France are also briefly discussed...
  10. ncbi request reprint Current prophylactic and therapeutic uses of a recombinant Lactococcus lactis strain secreting biologically active interleukin-12
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Jouy en Josas, France
    J Mol Microbiol Biotechnol 14:80-9. 2008
    ..Three well-illustrated examples demonstrate the high potential of interleukin-12-secreting lactococci strains for future prophylactic and therapeutic uses...
  11. doi request reprint Production of biological active murine IFN-gamma by recombinant Lactococcus lactis
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, Domaine de Vilvert, Jouy en Josas, France
    FEMS Microbiol Lett 280:144-9. 2008
    ..lactis, is able to produce functional muIFN-gamma. This recombinant strain could lead to the development of a new, well-tolerated vector to deliver active muIFN-gamma at the mucosal level...
  12. pmc Impairment of the intestinal barrier by ethanol involves enteric microflora and mast cell activation in rodents
    Laurent Ferrier
    Unité de Neuro Gastroentérologie et Nutrition, Institut National de la Recherche Agronomique, 180 chemin de Tournefeuille, B P 3, 31931 Toulouse Cedex 9, France
    Am J Pathol 168:1148-54. 2006
    ..Such alterations that remain for longer periods could result in excessive endotoxin passage, which could explain the subsequent endotoxemia frequently observed in patients with alcoholic liver disease...
  13. doi request reprint Variations of N-acetylation level of peptidoglycan do not influence persistence of Lactococcus lactis in the gastrointestinal tract
    Laurie Watterlot
    UMR 1319 MICALIS Microbiologie de l Alimentation au Service de la Santé humaine, F 78350, Jouy en Josas, France
    Int J Food Microbiol 144:29-34. 2010
    ..lactis leading to different levels of PG deacetylation does not confer any advantage in the persistence of this bacterium in the GIT and its ability to enhance host immune responses induced by delivered antigen in situ...
  14. pmc Effects of intranasal administration of a leptin-secreting Lactococcus lactis recombinant on food intake, body weight, and immune response of mice
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 73:5300-7. 2007
    ..These results demonstrate that leptin can be produced and secreted in an active form by L. lactis and that leptin-producing L. lactis regulates in vivo antigen-specific immune responses, as well as body weight and food consumption...
  15. pmc Use of native lactococci as vehicles for delivery of DNA into mammalian epithelial cells
    Valeria Dellaretti Guimarães
    Unité de Virologie et Immunologie Moléculaire, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 72:7091-7. 2006
    ..BLG secretion by Caco-2 cells started 48 h after coincubation with MG1363(pLIG:BLG1). We conclude that lactococci can deliver BLG cDNA into mammalian epithelial cells, demonstrating their potential to deliver in vivo a DNA vaccine...
  16. pmc Intranasal immunization with recombinant Lactococcus lactis secreting murine interleukin-12 enhances antigen-specific Th1 cytokine production
    Luis G Bermúdez-Humarán
    Unité de Recherches Laitières et de Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Infect Immun 71:1887-96. 2003
    ..Our data show that IL-12 is produced and secreted in an active form by L. lactis and that the strategy which we describe can be used to enhance an antigen-specific immune response and to stimulate local mucosal immunity...
  17. pmc In vitro and in vivo characterization of DNA delivery using recombinant Lactococcus lactis expressing a mutated form of L. monocytogenes Internalin A
    Marcela de Azevedo
    INRA, UMR1319 MICALIS, Commensals and Probiotics Host Interactions Laboratory, Jouy en Josas, France
    BMC Microbiol 12:299. 2012
    ..In order to avoid the limitations of LL-InlA+ and LL-FnBPA+, a new L. lactis strain was engineered to produce a previously described mutated form of InlA (LL-mInlA+) allowing the binding of mInlA on murine E-cadherin...
  18. doi request reprint Engineering lactococci and lactobacilli for human health
    Luis G Bermúdez-Humarán
    INRA, Commensal and Probiotics Host Interactions Laboratory, UMR 1319 Micalis, F 78350 Jouy en Josas, France AgroParisTech, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    Curr Opin Microbiol 16:278-83. 2013
    ..Further tests and in particular human clinical trials are now important next steps to conclude on the benefit of these approaches for human health. ..
  19. pmc Role of commensal and probiotic bacteria in human health: a focus on inflammatory bowel disease
    Rebeca Martín
    INRA, UMR1319 MICALIS, Jouy en Josas, F 78350, France
    Microb Cell Fact 12:71. 2013
    ..Our aim is to highlight the potential roles of recombinant bacteria in this ecosystem. ..
  20. doi request reprint The intestinal microbiota in the rat model: major breakthroughs from new technologies
    Julie Tomas
    Commensal and Probiotics Host Interactions Laboratory, INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    Anim Health Res Rev 13:54-63. 2012
    ..Determining the non-pathogenic microbial community might be relevant in standards of quality control of laboratory animals. Molecular profiling techniques could be applied to document this information...
  21. ncbi request reprint Heterologous protein production and delivery systems for Lactococcus lactis
    Sebastien Nouaille
    Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Genet Mol Res 2:102-11. 2003
    ..The expression of heterologous proteins and antigens as well as the various delivery systems developed in L. lactis, and its use as an oral vaccine carrier are discussed...
  22. ncbi request reprint Cell-surface display of E7 antigen from human papillomavirus type-16 in Lactococcus lactis and in Lactobacillus plantarum using a new cell-wall anchor from lactobacilli
    Naima G Cortes-Perez
    Unité de Recherches Laitières et de Génétique Appliquée, INRA, Domaine de Vilvert, 78352, Jouy en Josas, Cedex, France
    J Drug Target 13:89-98. 2005
    ..The E7mm production and cell-surface display in both L. lactis and a probiotic bacterium, Lb. plantarum, represent one more step towards the development of a safe and effective treatment against CxCa...
  23. doi request reprint Allergy therapy by intranasal administration with recombinant Lactococcus lactis Producing bovine beta-lactoglobulin
    Naima G Cortes-Perez
    INRA, UR0496, Unité d Immuno Allergie Alimentaire, CEA SPI, iBiTecS, Gif sur Yvette, France
    Int Arch Allergy Immunol 150:25-31. 2009
    ..This study aimed to evaluate the effects of the LL-BLG strain in a therapeutic protocol...
  24. ncbi request reprint Antimicrobial activity of divercin RV41 produced and secreted by Lactococcus lactis
    Luis G Bermúdez-Humarán
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, Jouy en Josas, France
    J Mol Microbiol Biotechnol 13:259-63. 2007
    ..In conclusion, our data show that the bacteriocin DvnRV41 is produced and secreted in an active form by L. lactis and that this approach may have important applications in the preservation of foods...
  25. pmc Identification of one novel candidate probiotic Lactobacillus plantarum strain active against influenza virus infection in mice by a large-scale screening
    Noura Kechaou
    INCommensal and Probiotics Host Interactions Laboratory, Micalis, Jouy en Josas, France
    Appl Environ Microbiol 79:1491-9. 2013
    ..Moreover, we identified a novel candidate probiotic strain, L. plantarum CNRZ1997, active against influenza virus infection in mice...
  26. doi request reprint Microbiota matures colonic epithelium through a coordinated induction of cell cycle-related proteins in gnotobiotic rat
    Claire Cherbuy
    Institut National de la Recherche Agronomique, Micalis, UMR, Pôle Ecosystèmes, Jouy en Josas, France
    Am J Physiol Gastrointest Liver Physiol 299:G348-57. 2010
    ..This event takes place in conjunction with a multistep process: a hyperproliferative boost accompanied by compensatory events as induction of p21(cip1) and p27(kip1) and decrease of Bcl2...
  27. pmc Characterization of O-acetylation of N-acetylglucosamine: a novel structural variation of bacterial peptidoglycan
    Elvis Bernard
    INRA, UMR1319 MICALIS, F 78350 Jouy en Josas, France
    J Biol Chem 286:23950-8. 2011
    ..Our data indicate that in L. plantarum, two different O-acetyltransferases play original and antagonistic roles in the modulation of the activity of endogenous autolysins...
  28. doi request reprint Thiostrepton tryptophan methyltransferase expands the chemistry of radical SAM enzymes
    Stéphane Pierre
    Institut National de la Recherche Agronomique, UMR 1319 Micalis, Jouy en Josas, France
    Nat Chem Biol 8:957-9. 2012
    ..We show that it uses unprecedented chemistry to methylate inactivated sp(2)-hybridized carbon atoms, despite being predicted to be a radical SAM enzyme...
  29. ncbi request reprint Internalin-expressing Lactococcus lactis is able to invade small intestine of guinea pigs and deliver DNA into mammalian epithelial cells
    Valeria Dellaretti Guimarães
    Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Microbes Infect 7:836-44. 2005
    ..The L. lactis inlA+ strain will be a useful bacterial vector for the development of new live oral DNA vaccines. It also constitutes an interesting new model to study the role of internalin in bacterial localization in the animal host...
  30. ncbi request reprint Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis
    Luis G Bermúdez-Humarán
    Unité de Recherches Laitières et de Génétique Appliquée, INRA, Domaine de Vilvert, 78352 Cedex, Jouy en Josas, France
    FEMS Microbiol Lett 224:307-13. 2003
    ..The SE and the quantity of secreted active IFN-omega were evaluated respectively to be approximately 70% and approximately 1 mg x l(-1) ( approximately two-fold higher than the cytoplasmic form)...
  31. pmc Analysis of Lactobacillus sakei mutants selected after adaptation to the gastrointestinal tracts of axenic mice
    Fabrizio Chiaramonte
    Unité Flore Lactique et Environnement Carné, UR309, INRA, Domaine de Vilvert, Jouy en Josas, France
    Appl Environ Microbiol 76:2932-9. 2010
    ....
  32. pmc Lactococcus lactis expressing either Staphylococcus aureus fibronectin-binding protein A or Listeria monocytogenes internalin A can efficiently internalize and deliver DNA in human epithelial cells
    Silvia Innocentin
    Unite de Virologie et Immunologie Moleculaires, UR892 INRA, Domaine de Vilvert, F78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 75:4870-8. 2009
    ..lactis FnBPA(+) or L. lactis InlA(+). Analysis of the in vivo efficiency of these invasive recombinant strains is currently in progress to validate their potential as DNA vaccine delivery vehicles...
  33. pmc Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients
    Harry Sokol
    Unité Ecologie et Physiologie du Système Digestif, Institut National de la Recherche Agronomique U910, Domaine de Vilvert, 78350 Jouy en Josas, France
    Proc Natl Acad Sci U S A 105:16731-6. 2008
    ..These results suggest that counterbalancing dysbiosis using F. prausnitzii as a probiotic is a promising strategy in CD treatment...
  34. pmc Production of a heterologous nonheme catalase by Lactobacillus casei: an efficient tool for removal of H2O2 and protection of Lactobacillus bulgaricus from oxidative stress in milk
    Tatiana Rochat
    Unité d Ecologie et de Physiologie du Système Digestif, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 72:5143-9. 2006
    ..In mixtures of L. casei producing MnKat and L. bulgaricus, L. casei can eliminate H2O2 from the culture medium, thereby protecting both L. casei and L. bulgaricus from its deleterious effects...
  35. pmc Lactococcus lactis SpOx spontaneous mutants: a family of oxidative-stress-resistant dairy strains
    Tatiana Rochat
    Unité de Recherches Laitières et de Génetique, INRA, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 71:2782-8. 2005
    ..delbrueckii strains. Furthermore, the transit kinetics of the SpOx3 mutant in the digestive tract of a human flora-associated mouse model was not affected...
  36. pmc Production and targeting of the Brucella abortus antigen L7/L12 in Lactococcus lactis: a first step towards food-grade live vaccines against brucellosis
    Luciana A Ribeiro
    Laboratoire de Génétique Appliquée, Unité de Recherches Laitières et de Génétique Appliquée, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas Cedex, France
    Appl Environ Microbiol 68:910-6. 2002
    ..lactis. This is the first example of a B. abortus antigen produced in a food-grade bacterium and opens new perspectives for alternative vaccine strategies against brucellosis...