Jean Dubuisson

Summary

Affiliation: Institut de Biologie de Lille
Country: France

Publications

  1. pmc NS2 protein of hepatitis C virus interacts with structural and non-structural proteins towards virus assembly
    Costin Ioan Popescu
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille CIIL, Institut Pasteur de Lille, Universite Lille Nord de France, Lille, France
    PLoS Pathog 7:e1001278. 2011
  2. pmc The CD81 partner EWI-2wint inhibits hepatitis C virus entry
    Vera Rocha-Perugini
    Institut de Biologie de Lille UMR8161, CNRS, Universités de Lille I et Lille II, Institut Pasteur de Lille, Lille, France
    PLoS ONE 3:e1866. 2008
  3. pmc Recent advances in hepatitis C virus cell entry
    Birke Bartosch
    INSERM, U871, 69003 Lyon, France
    Viruses 2:692-709. 2010
  4. pmc Enhanced anti-HCV activity of interferon alpha 17 subtype
    Aurelie Dubois
    Virology Laboratory Amiens University Medical Centre, France
    Virol J 6:70. 2009
  5. pmc New pandemics: HIV and AIDS, HCV and chronic hepatitis, influenza virus and flu
    Anne Gatignol
    Lady Davis Institute for Medical Research, and Department of Medicine, Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Retrovirology 4:8. 2007
  6. ncbi request reprint Glycosylation of the hepatitis C virus envelope protein E1 is dependent on the presence of a downstream sequence on the viral polyprotein
    J Dubuisson
    CNRS Unité Mixte de Recherche 8526, Institut de Biologie de Lille Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Biol Chem 275:30605-9. 2000
  7. ncbi request reprint Interaction of hepatitis C virus proteins with host cell membranes and lipids
    Jean Dubuisson
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    Trends Cell Biol 12:517-23. 2002
  8. ncbi request reprint Hepatitis C virus proteins
    Jean Dubuisson
    Hepatitis C Laboratory, CNRS UMR8161, Institut de Biologie de Lille I and II, Universite de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    World J Gastroenterol 13:2406-15. 2007
  9. ncbi request reprint Early steps of the hepatitis C virus life cycle
    Jean Dubuisson
    Institut de Biologie de Lille UMR8161, CNRS, Université de Lille I and II and Institut Pasteur de Lille, Lille, France
    Cell Microbiol 10:821-7. 2008
  10. pmc Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus
    Yves Rouille
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:2832-41. 2006

Collaborators

Detail Information

Publications80

  1. pmc NS2 protein of hepatitis C virus interacts with structural and non-structural proteins towards virus assembly
    Costin Ioan Popescu
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille CIIL, Institut Pasteur de Lille, Universite Lille Nord de France, Lille, France
    PLoS Pathog 7:e1001278. 2011
    ..Together, these observations indicate that NS2 protein attracts the envelope proteins at the assembly site and it crosstalks with non-structural proteins for virus assembly...
  2. pmc The CD81 partner EWI-2wint inhibits hepatitis C virus entry
    Vera Rocha-Perugini
    Institut de Biologie de Lille UMR8161, CNRS, Universités de Lille I et Lille II, Institut Pasteur de Lille, Lille, France
    PLoS ONE 3:e1866. 2008
    ..This is the first example of a pathogen gaining entry into host cells that lack a specific inhibitory factor...
  3. pmc Recent advances in hepatitis C virus cell entry
    Birke Bartosch
    INSERM, U871, 69003 Lyon, France
    Viruses 2:692-709. 2010
    ..We discuss the roles of these cellular factors in HCV cell entry and how association of HCV with lipoproteins may modulate the cell entry process...
  4. pmc Enhanced anti-HCV activity of interferon alpha 17 subtype
    Aurelie Dubois
    Virology Laboratory Amiens University Medical Centre, France
    Virol J 6:70. 2009
    ..Interferon alpha is a type I interferon composed of 12 different subtypes. Each subtype signals by the Jak-Stat pathway but modulations in the antiviral activity was previously described...
  5. pmc New pandemics: HIV and AIDS, HCV and chronic hepatitis, influenza virus and flu
    Anne Gatignol
    Lady Davis Institute for Medical Research, and Department of Medicine, Microbiology and Immunology, McGill University, Montreal, Quebec, Canada
    Retrovirology 4:8. 2007
    ..Aspects of viral structure, virus-host interactions, antiviral defenses, drugs and vaccinations, and epidemiological aspects were discussed for HIV and HCV. Old and recent data on the flu epidemics ended this meeting...
  6. ncbi request reprint Glycosylation of the hepatitis C virus envelope protein E1 is dependent on the presence of a downstream sequence on the viral polyprotein
    J Dubuisson
    CNRS Unité Mixte de Recherche 8526, Institut de Biologie de Lille Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Biol Chem 275:30605-9. 2000
    ..These data indicate that glycosylation of E1 is dependent on the presence of polypeptide sequences located downstream of E1 on HCV polyprotein...
  7. ncbi request reprint Interaction of hepatitis C virus proteins with host cell membranes and lipids
    Jean Dubuisson
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    Trends Cell Biol 12:517-23. 2002
    ....
  8. ncbi request reprint Hepatitis C virus proteins
    Jean Dubuisson
    Hepatitis C Laboratory, CNRS UMR8161, Institut de Biologie de Lille I and II, Universite de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    World J Gastroenterol 13:2406-15. 2007
    ..This review summarizes the current knowledge on the functions and biochemical features of HCV proteins...
  9. ncbi request reprint Early steps of the hepatitis C virus life cycle
    Jean Dubuisson
    Institut de Biologie de Lille UMR8161, CNRS, Université de Lille I and II and Institut Pasteur de Lille, Lille, France
    Cell Microbiol 10:821-7. 2008
    ..The current knowledge accumulated on HCV entry is summarized in this review...
  10. pmc Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus
    Yves Rouille
    CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:2832-41. 2006
    ..In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle...
  11. pmc The neutralizing activity of anti-hepatitis C virus antibodies is modulated by specific glycans on the E2 envelope protein
    François Helle
    Institut de Biologie de Lille UMR8161, CNRS, Université Lille I and II and Institut Pasteur de Lille, Lille, France
    J Virol 81:8101-11. 2007
    ..In conclusion, this work indicates that HCV glycans contribute to the evasion of HCV from the humoral immune response...
  12. doi request reprint Role of low-density lipoprotein receptor in the hepatitis C virus life cycle
    Anna Albecka
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, Lille, France
    Hepatology 55:998-1007. 2012
    ..Conclusion: The LDLR is not essential for infectious HCV particle entry, whereas the physiological function of this receptor is important for optimal replication of the HCV genome...
  13. pmc Interacting regions of CD81 and two of its partners, EWI-2 and EWI-2wint, and their effect on hepatitis C virus infection
    Claire Montpellier
    Center for Infection and Immunity of Lille, Hepatitis C Laboratory, University Lille Nord de France, CNRS UMR8204, Inserm U1019, Pasteur Institute of Lille, 59021 Lille, France
    J Biol Chem 286:13954-65. 2011
    ..Finally, we identified the regions in CD81 that are necessary for its functionality in HCV entry and we demonstrated that EWI-2wint needs to interact with CD81 to exert its inhibitory effect on HCV infection...
  14. pmc The association of CD81 with tetraspanin-enriched microdomains is not essential for Hepatitis C virus entry
    Vera Rocha-Perugini
    Institut de Biologie de Lille, CNRS UMR8161, Universite Lille Nord de France, Institut Pasteur de Lille, Lille, France
    BMC Microbiol 9:111. 2009
    ..Interestingly, CD81 is also required for Plasmodium infection. A major characteristic of tetraspanins is their ability to interact with each other and other transmembrane proteins to build tetraspanin-enriched microdomains (TEM)...
  15. doi request reprint (-)-Epigallocatechin-3-gallate is a new inhibitor of hepatitis C virus entry
    Noémie Calland
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille CIIL, Lille, France
    Hepatology 55:720-9. 2012
    ..Finally, by successive inoculation of naïve cells with supernatant of HCV-infected cells in the presence of EGCG, we observed that EGCG leads to undetectable levels of infection after four passages...
  16. pmc Role of N-linked glycans in the functions of hepatitis C virus envelope proteins incorporated into infectious virions
    François Helle
    Institut Pasteur de Lille, Bâtiment IBL, 59021 Lille Cedex, France
    J Virol 84:11905-15. 2010
    ..Furthermore, these carbohydrates form a "glycan shield" at the surface of the virion, which contributes to the evasion of HCV from the humoral immune response...
  17. pmc Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins
    Anne Goffard
    CNRS UPR2511, Institut de Biologie de Lille, France
    J Virol 79:8400-9. 2005
    ..Altogether, the data indicate that some glycans of HCV envelope glycoproteins play a major role in protein folding and others play a role in HCV entry...
  18. ncbi request reprint High density lipoprotein inhibits hepatitis C virus-neutralizing antibodies by stimulating cell entry via activation of the scavenger receptor BI
    Marlene Dreux
    INSERM, U758, 69007 Lyon, France
    J Biol Chem 281:18285-95. 2006
    ..Consistently, we show that antibodies targeted to HCV-E1 efficiently neutralize HCVpp and HCVcc in the presence of human serum...
  19. ncbi request reprint Contribution of the charged residues of hepatitis C virus glycoprotein E2 transmembrane domain to the functions of the E1E2 heterodimer
    Yann Ciczora
    CNRS UPR2511, Unité Hépatite C, Institut de Biologie de Lille, 1 rue Calmette, BP 447, 59021 Lille Cedex, France
    J Gen Virol 86:2793-8. 2005
    ..However, the Asp and Arg residues do not contribute equally to these functions...
  20. ncbi request reprint Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins
    David Delgrange
    CNRS UMR 8161, IBL, Université de Lille I et Lille II, Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Gen Virol 88:2495-503. 2007
    ..Altogether, our data indicate that a more robust production of HCVcc particles can be obtained by introducing a few specific mutations in JFH-1 structural proteins...
  21. pmc Identification of GBF1 as a cellular factor required for hepatitis C virus RNA replication
    Lucie Goueslain
    CNRS UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
    J Virol 84:773-87. 2010
    ..Altogether, our results highlight a functional connection between the early secretory pathway and HCV RNA replication...
  22. ncbi request reprint Cyanovirin-N inhibits hepatitis C virus entry by binding to envelope protein glycans
    François Helle
    Centre National de la Recherche Scientifique, Institut de Biologie de Lille Unité Mixte de Recherche 8161, Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Biol Chem 281:25177-83. 2006
    ..Furthermore, CV-N is a new invaluable tool to further dissect the early steps of HCV entry into host cells...
  23. ncbi request reprint Regulation of hepatitis C virus polyprotein processing by signal peptidase involves structural determinants at the p7 sequence junctions
    Séverine Carrère-Kremer
    CNRS UPR2511, Institut de Biologie de Lille, 59021 Lille, France
    J Biol Chem 279:41384-92. 2004
    ..Such constraints in the processing of a polyprotein precursor are likely essential for hepatitis C virus to post-translationally regulate the kinetics and/or the level of expression of p7 as well as NS2 and E2 mature proteins...
  24. pmc Hepatitis C virus entry depends on clathrin-mediated endocytosis
    Emmanuelle Blanchard
    Equipe Hepatite C, CNRS UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:6964-72. 2006
    ..These data indicate that HCV enters target cells by clathrin-mediated endocytosis, followed by a fusion step from within an acidic endosomal compartment...
  25. ncbi request reprint High-density lipoproteins reduce the neutralizing effect of hepatitis C virus (HCV)-infected patient antibodies by promoting HCV entry
    Cécile Voisset
    CNRS, Institut de Biologie de Lille UMR8161, Institut Pasteur de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Gen Virol 87:2577-81. 2006
    ..Altogether, these observations indicate that HCV is exploiting the physiological activity of SR-BI for promoting its entry into target cells, which consequently also protects the virus against neutralizing antibodies...
  26. ncbi request reprint Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitors
    Cynthia Chapel
    INSERM, U871, Universite Lyon 1, et IFR62 Laennec, Lyon, France
    J Gen Virol 88:1133-43. 2007
    ..These properties suggest the potential usefulness of DNJ derivatives in combating HCV infection...
  27. doi request reprint Identification of a dominant endoplasmic reticulum-retention signal in yellow fever virus pre-membrane protein
    Yann Ciczora
    Universite Lille Nord de France, F 59000 Lille, France
    J Gen Virol 91:404-14. 2010
    ..Together, these data indicate that a combination of several signals of different strengths contributes to the ER retention of the YFV envelope protein heterodimer...
  28. pmc Topological changes in the transmembrane domains of hepatitis C virus envelope glycoproteins
    Laurence Cocquerel
    CNRS UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    EMBO J 21:2893-902. 2002
    ....
  29. pmc Characterization of the envelope glycoproteins associated with infectious hepatitis C virus
    Gabrielle Vieyres
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, F 59019 Lille, France
    J Virol 84:10159-68. 2010
    ..Overall, our study fills a gap in the description of HCV outer morphology and should guide further investigations into virus entry and assembly...
  30. pmc Hepatitis C virus replication and Golgi function in brefeldin a-resistant hepatoma-derived cells
    Rayan Farhat
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille CIIL, Institut Pasteur de Lille, Universite Lille Nord de France, Lille, France
    PLoS ONE 8:e74491. 2013
    ..Thus, our results confirm the involvement of GBF1 in HCV replication, and suggest that GBF1 might fulfill another function, in addition to the regulation of the secretory pathway, during HCV replication. ..
  31. doi request reprint The antimalarial ferroquine is an inhibitor of hepatitis C virus
    Thibaut Vausselin
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, Lille, France
    Hepatology 58:86-97. 2013
    ..Conclusion: FQ is a novel, interesting anti-HCV molecule that could be used in combination with other direct-acting antivirals...
  32. pmc Identification of new functional regions in hepatitis C virus envelope glycoprotein E2
    Anna Albecka
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, F 59019 Lille, France
    J Virol 85:1777-92. 2011
    ..In conclusion, our study highlights new functional and structural regions in HCV envelope glycoprotein E2...
  33. pmc Identification of basic amino acids at the N-terminal end of the core protein that are crucial for hepatitis C virus infectivity
    Khaled Alsaleh
    Inserm U1019, CNRS UMR 8204, Center for Infection and Immunity of Lille, Institut de Biologie de Lille, F 59021 Lille, France
    J Virol 84:12515-28. 2010
    ..Together, these data indicate that R50, K51, R59, and R62 residues play a major role in the formation of infectious viral particles at a post-nucleocapsid assembly step...
  34. ncbi request reprint Ceramide enrichment of the plasma membrane induces CD81 internalization and inhibits hepatitis C virus entry
    Cécile Voisset
    Institut de Biologie de Lille UMR8161, CNRS, Université de Lille I and II and Institut Pasteur de Lille, Lille, France
    Cell Microbiol 10:606-17. 2008
    ..Together, these data indicate that some specific lipids of the plasma membrane are essential for HCV entry and highlight plasma membrane lipids as potential targets to block HCV entry...
  35. pmc Basic residues in hypervariable region 1 of hepatitis C virus envelope glycoprotein e2 contribute to virus entry
    Nathalie Callens
    Unité Hépatite C, CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 79:15331-41. 2005
    ..Despite the lack of evidence of the involvement of known potential receptors, our results demonstrate that the presence of basic residues in HVR1 facilitates virus entry...
  36. ncbi request reprint Hepatitis C virus entry: potential receptors and their biological functions
    Laurence Cocquerel
    CNRS UMR8161, Institut de Biologie de Lille, Institut Pasteur de Lille, France
    J Gen Virol 87:1075-84. 2006
    ..In this review, the molecules that have been proposed as potential HCV receptors are described and the experimental data indicating that CD81 and SR-BI are potentially involved in HCV entry are presented...
  37. pmc Griffithsin has antiviral activity against hepatitis C virus
    Philip Meuleman
    Institut Pasteur de Lille, Center for Infection and Immunity of Lille, 1 rue Calmette, 59021 Lille Cedex, France
    Antimicrob Agents Chemother 55:5159-67. 2011
    ..GRFT treatment of chronically infected HCV patients undergoing liver transplantation may be a suitable strategy to prevent infection of the liver allograft...
  38. pmc Disulfide bonds in hepatitis C virus glycoprotein E1 control the assembly and entry functions of E2 glycoprotein
    Ahmed Wahid
    Center for Infection and Immunity of Lille, Inserm U1019, CNRS UMR8204, Institut Pasteur de Lille, Universite Lille Nord de France, Lille, France
    J Virol 87:1605-17. 2013
    ..Therefore, in the context of HCV, our data identify an additional function of a class II companion protein as a molecule that can control the binding capacity of the fusion protein...
  39. ncbi request reprint High density lipoproteins facilitate hepatitis C virus entry through the scavenger receptor class B type I
    Cécile Voisset
    CNRS UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, Lille 59021, France
    J Biol Chem 280:7793-9. 2005
    ..Altogether, these data indicate that HDL-mediated enhancement of HCVpp entry involves a complex interplay between SR-BI, HDL, and HCV envelope glycoproteins, and they highlight the active role of HDLs in HCV entry...
  40. ncbi request reprint Serum amyloid A has antiviral activity against hepatitis C virus by inhibiting virus entry in a cell culture system
    Muriel Lavie
    Hepatitis C Laboratory, CNRS Institut de Biologie de Lille UMR8161 and Institut Pasteur de Lille, Lille, France
    Hepatology 44:1626-34. 2006
    ..In conclusion, our data demonstrate an antiviral activity for SAA and suggest a tight relationship between SAA and HDL in modulating HCV infectivity...
  41. ncbi request reprint Characterization of the expression of the hepatitis C virus F protein
    Juliette Roussel
    CNRS UPR 2511, IBL Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Gen Virol 84:1751-9. 2003
    ..Finally, indirect immunofluorescence studies have localized the F protein in the cytoplasm, with notable perinuclear detection...
  42. doi request reprint EWI-2wint promotes CD81 clustering that abrogates Hepatitis C Virus entry
    Julie Potel
    Hepatitis C Laboratory, Center for Infection and Immunity of Lille, University Lille Nord de France, CNRS UMR8204, Inserm U1019, Pasteur Institute of Lille, Lille, France
    Cell Microbiol 15:1234-52. 2013
    ..This study gives new insights on the mechanism by which HCV enters into its target cells, namely by exploiting the dynamic properties of CD81...
  43. pmc Hepatitis C virus and natural compounds: a new antiviral approach?
    Noémie Calland
    Inserm U1019, CNRS UMR8204, Center for Infection and Immunity of Lille, Institut Pasteur de Lille, Universite Lille Nord de France, Lille, France
    Viruses 4:2197-217. 2012
    ..Recent reports about natural compounds highlight their antiviral activity against HCV. Here, we aim to review the natural molecules that interfere with the HCV life cycle and discuss their potential use in HCV therapy...
  44. pmc Transmembrane domains of hepatitis C virus envelope glycoproteins: residues involved in E1E2 heterodimerization and involvement of these domains in virus entry
    Yann Ciczora
    Hepatitis C Laboratory, CNRS UMR8161, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 81:2372-81. 2007
    ..In conclusion, this mutational analysis identified residues involved in E1E2 heterodimerization and revealed that the TM domains of HCV envelope glycoproteins play a major role in the fusion properties of these proteins...
  45. pmc Characterization of functional hepatitis C virus envelope glycoproteins
    Anne Op De Beeck
    CNRS UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, Lille, France
    J Virol 78:2994-3002. 2004
    ..The functional envelope glycoproteins associated with HCV pseudotype particles were also shown to be sensitive to low-pH treatment. Such conformational changes are likely necessary to initiate fusion...
  46. ncbi request reprint Assembly of a functional HCV glycoprotein heterodimer
    Muriel Lavie
    Institut de Biologie de Lille UMR8161, CNRS, Université de Lille I and II, and Institut Pasteur de Lille, Lille, France
    Curr Issues Mol Biol 9:71-86. 2007
    ..Here, we present the recent data that have been accumulated on the assembly of the functional HCV glycoprotein heterodimer...
  47. pmc The transmembrane domains of the prM and E proteins of yellow fever virus are endoplasmic reticulum localization signals
    Anne Op De Beeck
    Unité Hépatite C, CNRS UPR2511, Institut de Biologie de Lille, 1 rue Calmette, BP447, 59021 Lille Cedex, France
    J Virol 78:12591-602. 2004
    ..In addition, our data show that the mechanisms of ER retention of the flavivirus and hepacivirus envelope proteins are different...
  48. pmc Subcellular localization and topology of the p7 polypeptide of hepatitis C virus
    Séverine Carrère-Kremer
    CNRS FRE2369, Institut de Biologie de Lille Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Virol 76:3720-30. 2002
    ..Altogether, these data indicate that p7 is a polytopic membrane protein that could have a functional role in several compartments of the secretory pathway...
  49. ncbi request reprint Interactions between virus proteins and host cell membranes during the viral life cycle
    Rodrigo A Villanueva
    CNRS UPR2511, Institut de Biologie de Lille, Institut Pasteur de Lille, 59021 Lille Cedex, France
    Int Rev Cytol 245:171-244. 2005
    ..This paper reviews recent findings on the interactions of viral proteins with host cell membranes during the viral life cycle...
  50. doi request reprint Hepatocyte-derived cultured cells with unusual cytoplasmic keratin-rich spheroid bodies
    Pierre Yves Delavalle
    Inserm U1019, CNRS UMR 8204, CIIL, F 59021 Lille, France
    Exp Cell Res 317:2683-94. 2011
    ..In conclusion, our data suggest that Huh-7w7.3 cells constitute an excellent model for determining the cellular factor(s) involved in the process of spheroid perinuclear body formation...
  51. ncbi request reprint Another putative receptor for hepatitis C virus
    Anne Op De Beeck
    Institut de Biologie de Lille Institut Pasteur de Lille, Lille, France
    Hepatology 37:705-7. 2003
  52. ncbi request reprint Topology of hepatitis C virus envelope glycoproteins
    Anne Op De Beeck
    CNRS UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, 59021 Lille, France
    Rev Med Virol 13:233-41. 2003
    ....
  53. ncbi request reprint Glycosylation of hepatitis C virus envelope proteins
    Anne Goffard
    CNRS UPR2511, Institut de Biologie de Lille, Institut Pasteur de Lille, Lille, France
    Biochimie 85:295-301. 2003
    ..The knowledge that has been accumulated on the early steps of glycosylation of these proteins is presented in this review...
  54. doi request reprint Role of lipid metabolism in hepatitis C virus assembly and entry
    Costin Ioan Popescu
    Universite Lille Nord de France, Lille, France
    Biol Cell 102:63-74. 2010
    ..The present review aims to summarize the advances in our understanding of the HCV-lipid metabolism association, which may open new therapeutic avenues...
  55. pmc Role of the transmembrane domains of prM and E proteins in the formation of yellow fever virus envelope
    Anne Op De Beeck
    CNRS UPR2511 INSERM IFR17, Institut de Biologie de Lille Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Virol 77:813-20. 2003
    ..In addition, these data indicate some differences between the transmembrane domains of the hepaciviruses and the flaviviruses...
  56. ncbi request reprint Functional hepatitis C virus envelope glycoproteins
    Cécile Voisset
    CNRS UPR2511, Institut de Biologie de Lille and Institut Pasteur de Lille, 59021 Lille Cedex, France
    Biol Cell 96:413-20. 2004
    ....
  57. ncbi request reprint The alphavirus 6K protein activates endogenous ionic conductances when expressed in Xenopus oocytes
    Anne Frédérique Antoine
    Equipe d Accueil 4020, Institut Fédératif de la Recherche 147, Universite de Lille, 59655 Villeneuve d Ascq cedex, France
    J Membr Biol 215:37-48. 2007
    ..Our data confirm that 6K specifically triggers a sequential cascade of events that leads to cytoplasmic calcium elevation and cell permeabilization, which likely play a role in the Sindbis virus life cycle...
  58. pmc Bovine viral diarrhea virus entry is dependent on clathrin-mediated endocytosis
    Steve Lecot
    CNRS UPR2511, Institut de Biologie de Lille, Lille Cedex, France
    J Virol 79:10826-9. 2005
    ..Together, these data indicate that BVDV infection requires an active clathrin-dependent endocytic pathway...
  59. pmc Last stop before exit - hepatitis C assembly and release as antiviral drug targets
    Birke Andrea Tews
    Hepatitis C Laboratory, Center of Infection and Immunity of Lille, University Lille Nord de France, CNRS UMR8204, Inserm U1019, Pasteur Institute of Lille, 1, rue du Professeur Calmette, BP447, 59021 Lille, France E Mails C I P J D
    Viruses 2:1782-803. 2010
    ..This review summarizes the advances in our understanding of HCV particle assembly and the identification of new antiviral targets of potential interest in this late step of the HCV life cycle...
  60. ncbi request reprint Reconstitution of hepatitis C virus envelope glycoproteins into liposomes as a surrogate model to study virus attachment
    Michel Lambot
    CNRS Institut de Biologie de Lille and Institut Pasteur de Lille, 59021 Lille Cedex, France
    J Biol Chem 277:20625-30. 2002
    ..Together, these data indicate that E1E2-liposomes are a valuable tool to study the molecular requirements for HCV binding to target cells...
  61. pmc NMR structure and ion channel activity of the p7 protein from hepatitis C virus
    Roland Montserret
    Institut de Biologie et Chimie des Proteines, UMR 5086, CNRS, Universite de Lyon, IFR128 BioSciences Gerland Lyon Sud, 69367 Lyon, France
    J Biol Chem 285:31446-61. 2010
    ..These results provide the first detailed experimental structural framework for a better understanding of p7 processing, oligomerization, and ion channel gating mechanism...
  62. ncbi request reprint Cell entry of hepatitis C virus requires a set of co-receptors that include the CD81 tetraspanin and the SR-B1 scavenger receptor
    Birke Bartosch
    Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, Institut Fédératif de Recherche 128, Ecole Normale Superieure de Lyon, 46 allee d Italie, 69364 Lyon Cedex 07, France
    J Biol Chem 278:41624-30. 2003
    ..Finally, by correlating expression of HCV receptors and infectivity, we suggest that, besides CD81 and SR-B1, additional hepatocyte-specific co-factor(s) are necessary for HCV entry...
  63. pmc Analysis of a highly flexible conformational immunogenic domain a in hepatitis C virus E2
    Zhen Yong Keck
    Stanford Medical School Blood Center, 3373 Hillview Ave, Palo Alto, CA 94304, USA
    J Virol 79:13199-208. 2005
    ..Collectively, the organization and function of HCV E2 antigenic domains are roughly analogous to the large envelope glycoprotein E organizational structure for other flaviviruses with three distinct structural and functional domains...
  64. pmc Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus
    Daniel X Johansson
    Department of Medicine, Center for Molecular Medicine, Karolinska University Hospital Solna, Karolinska Institutet, 171 76 Stockholm, Sweden
    Proc Natl Acad Sci U S A 104:16269-74. 2007
    ....
  65. pmc Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes
    Birke Bartosch
    Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, Institut National de la Santé et de la Recherche Médicale U412, IFR 128, Ecole Normale Superieure de Lyon, 69364 Lyon Cedex 07, France
    J Exp Med 197:633-42. 2003
    ..Altogether, these studies indicate that these pseudo-particles may mimic the early infection steps of parental HCV and will be suitable for the development of much needed new antiviral therapies...
  66. ncbi request reprint Live and killed rhabdovirus-based vectors as potential hepatitis C vaccines
    Catherine A Siler
    The Dorrance H Hamilton Laboratories, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA
    Virology 292:24-34. 2002
    ..In addition, RV vaccine vehicles were able to induce cellular responses against HCV E2. These results further suggest that recombinant RVs are potentially useful vaccine vectors against important human viral diseases...
  67. pmc CD81-dependent binding of hepatitis C virus E1E2 heterodimers
    Laurence Cocquerel
    Department of Medicine, Stanford University Medical Center, Stanford, California 94305, USA
    J Virol 77:10677-83. 2003
    ..They also delineate a method by which to isolate biologically functional E1E2 complexes for the study of virus-host cell interaction in other cell types...
  68. ncbi request reprint Structural biology of hepatitis C virus
    Francois Penin
    Institut de Biologie et Chimie des Proteines, Lyon, France
    Hepatology 39:5-19. 2004
    ..This article reviews the current knowledge of HCV structural biology...
  69. pmc Hepatitis C virus (HCV)-induced immunoglobulin hypermutation reduces the affinity and neutralizing activities of antibodies against HCV envelope protein
    Keigo Machida
    Department of Molecular Microbiology and Immunology, University of Southern California, Keck School of Medicine, 2011 Zonal Avenue, Los Angeles, CA 90033, USA
    J Virol 82:6711-20. 2008
    ..These results suggest that HCV infection could cause some anti-HCV-antibody-producing hybridoma B cells to make less-protective antibodies...
  70. pmc Binding of hepatitis C virus-like particles derived from infectious clone H77C to defined human cell lines
    Sabine Wellnitz
    Department of Medicine II, University of Freiburg, Freiburg, Germany
    J Virol 76:1181-93. 2002
    ....
  71. ncbi request reprint Effect of ribavirin on the hepatitis C virus (JFH-1) and its correlation with interferon sensitivity
    Etienne Brochot
    Virology Laboratory, Amiens University Medical Centre, France
    Antivir Ther 12:805-13. 2007
    ..Such an accumulation of mutations would threaten the integrity of the virus's genetic information...
  72. pmc Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent
    Sonia Molina
    INSERM U632, Hepatic Physiopathology, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France
    J Virol 82:569-74. 2008
    ..5 cells revealed that the cell-virion combination is determinant of the entry process...
  73. ncbi request reprint Glycosylation of the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell line
    Sandrine Duvet
    CNRS UMR 8576 USTL, 59655 Villeneuve d Ascq cedex, France
    Glycobiology 12:95-101. 2002
    ..Comparisons with the N-glycosylation of other proteins expressed in B3F7 cells indicate that the posttranslational glycosylation of E1 is likely due to specific folding features of this acceptor protein...
  74. pmc CD81 expression is important for the permissiveness of Huh7 cell clones for heterogeneous hepatitis C virus infection
    Daisuke Akazawa
    Pharmaceutical Research Laboratories, Toray Industries, Inc, Kanagawa, Japan
    J Virol 81:5036-45. 2007
    ..In conclusion, CD81 expression is an important determinant of HCV permissiveness of Huh7 cell clones harboring different characteristics...
  75. ncbi request reprint Incomplete humoral immunity against hepatitis C virus is linked with distinct recognition of putative multiple receptors by E2 envelope glycoprotein
    Tae Hwe Heo
    Laboratory of Immunology, Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Shillim Dong, Kwanak gu, Seoul 151 742, Korea
    J Immunol 173:446-55. 2004
    ..Additionally, our data give crucial consideration to the development of HCV vaccines that stimulate protective humoral immune responses...
  76. doi request reprint Low levels of hepatitis C virus (HCV) neutralizing antibodies in patients coinfected with HCV and human immunodeficiency virus
    Sandrine Castelain
    Virologie, CHU Hopital Sud, Amiens, France
    J Infect Dis 198:332-5. 2008
    ..912+/-0.578) (P= .013). Lower HCV nAb titers in coinfected patients could help worsen the outcome of HCV infection. These results favor starting HCV therapy as soon as possible in coinfected patients...
  77. ncbi request reprint Antiviral effect of alpha-glucosidase inhibitors on viral morphogenesis and binding properties of hepatitis C virus-like particles
    Cynthia Chapel
    INSERM U271, Laboratoire des virus hépatiques et pathologies associées, 151 Cours Albert Thomas, 69424 Lyon Cedex 03, France
    J Gen Virol 87:861-71. 2006
    ..These alpha-glucosidase inhibitors may prove to be useful molecules to fight HCV infection in combination protocols...
  78. ncbi request reprint Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets
    Eriko Yamada
    The Edward Jenner Institute for Vaccine Research, Compton, Newbury RG20 7NN, UK
    J Gen Virol 86:2507-12. 2005
    ..However, those PBMC subsets reported to be infected by HCV in vivo (monocytes, DCs and B cells) also exhibited residual, CD81-independent binding, indicating roles for SR-BI/other receptor(s) in mediating haematopoietic cell infection...
  79. ncbi request reprint Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus
    Dimitri Lavillette
    Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, INSERM U412, IFR128, BioSciences Lyon Gerland, Ecole Normale Superieure de Lyon, Lyon, France
    Hepatology 41:265-74. 2005
    ..In conclusion, we characterize common steps in the cell entry pathways of the major HCV genotypes that should provide clues for the development of cell entry inhibitors and vaccines...
  80. pmc Hepatitis C virus E2 has three immunogenic domains containing conformational epitopes with distinct properties and biological functions
    Zhen Yong Keck
    Department of Pathology, Stanford University School of Medicine, Stanford, California, USA
    J Virol 78:9224-32. 2004
    ..Segregation of virus neutralization and sensitivity to low pH to specific regions supports a model of HCV E2 immunogenic domains similar to the antigenic structural and functional domains of other flavivirus envelope E glycoproteins...