Josef Deutscher

Summary

Affiliation: Institut National de la Recherche Agronomique
Country: France

Publications

  1. doi request reprint Correlations between carbon metabolism and virulence in bacteria
    Sandrine Poncet
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS AgroParisTech INRA, Thiverval Grignon, France
    Contrib Microbiol 16:88-102. 2009
  2. ncbi request reprint Ser/Thr/Tyr protein phosphorylation in bacteria - for long time neglected, now well established
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 9:125-31. 2005
  3. ncbi request reprint P-Ser-HPr--a link between carbon metabolism and the virulence of some pathogenic bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, F 78850 Thiverval Grignon, France
    Biochim Biophys Acta 1754:118-25. 2005
  4. pmc The Lactobacillus casei ptsHI47T mutation causes overexpression of a LevR-regulated but RpoN-independent operon encoding a mannose class phosphotransferase system
    Alain Mazé
    Laboratoire de Microbiologie et Genetique Moleculaire, INRA INAPG CNRS, Thiverval Grignon, France
    J Bacteriol 186:4543-55. 2004
  5. ncbi request reprint HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria
    Sandrine Poncet
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, F 78850 Thiverval Grignon, France
    Biochim Biophys Acta 1697:123-35. 2004
  6. ncbi request reprint How seryl-phosphorylated HPr inhibits PrfA, a transcription activator of Listeria monocytogenes virulence genes
    Rana Herro
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 9:224-34. 2005
  7. ncbi request reprint Transcription regulators potentially controlled by HPr kinase/phosphorylase in Gram-negative bacteria
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS UMR2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 5:206-15. 2003
  8. ncbi request reprint Is 2-phosphoglycerate-dependent automodification of bacterial enolases implicated in their export?
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG, UMR 2585, F 78850, Thiverval Grignon, France
    J Mol Biol 337:485-96. 2004
  9. ncbi request reprint How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF
    Ivan Mijakovic
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR 2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 8:19-25. 2004
  10. pmc Pyrophosphate-producing protein dephosphorylation by HPr kinase/phosphorylase: a relic of early life?
    Ivan Mijakovic
    Laboratoire de génétique des microorganismes, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1925, F 78850 Thiverval Grignon, France
    Proc Natl Acad Sci U S A 99:13442-7. 2002

Collaborators

Detail Information

Publications41

  1. doi request reprint Correlations between carbon metabolism and virulence in bacteria
    Sandrine Poncet
    Laboratoire de Microbiologie et Genetique Moleculaire, CNRS AgroParisTech INRA, Thiverval Grignon, France
    Contrib Microbiol 16:88-102. 2009
    ....
  2. ncbi request reprint Ser/Thr/Tyr protein phosphorylation in bacteria - for long time neglected, now well established
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 9:125-31. 2005
    ..Surprisingly, many bacterial protein kinases do not exhibit any similarity to eukaryotic protein kinases, but rather resemble nucleotide-binding proteins or kinases phosphorylating diverse low-molecular-weight substrates...
  3. ncbi request reprint P-Ser-HPr--a link between carbon metabolism and the virulence of some pathogenic bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, F 78850 Thiverval Grignon, France
    Biochim Biophys Acta 1754:118-25. 2005
    ..Indeed, inactivation of Neisseria meningitidis hprK strongly diminished cell adhesion of this pathogen...
  4. pmc The Lactobacillus casei ptsHI47T mutation causes overexpression of a LevR-regulated but RpoN-independent operon encoding a mannose class phosphotransferase system
    Alain Mazé
    Laboratoire de Microbiologie et Genetique Moleculaire, INRA INAPG CNRS, Thiverval Grignon, France
    J Bacteriol 186:4543-55. 2004
    ....
  5. ncbi request reprint HPr kinase/phosphorylase, a Walker motif A-containing bifunctional sensor enzyme controlling catabolite repression in Gram-positive bacteria
    Sandrine Poncet
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR2585, F 78850 Thiverval Grignon, France
    Biochim Biophys Acta 1697:123-35. 2004
    ..The structures of the complexes of HprK/P with HPr and P-Ser-HPr have also been determined, which allowed proposing a detailed mechanism for the kinase and phosphorylase functions of HprK/P...
  6. ncbi request reprint How seryl-phosphorylated HPr inhibits PrfA, a transcription activator of Listeria monocytogenes virulence genes
    Rana Herro
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 9:224-34. 2005
    ..In agreement with this concept, disruption of the enzyme I- or HPr-encoding genes also strongly inhibited PrfA activity. PrfA activity therefore seems to depend on a fully functional PTS phosphorylation cascade...
  7. ncbi request reprint Transcription regulators potentially controlled by HPr kinase/phosphorylase in Gram-negative bacteria
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS UMR2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 5:206-15. 2003
    ..We propose that HprK/P might control the phosphorylation state of HPr and EIIAs, which in turn could control the transcription regulators...
  8. ncbi request reprint Is 2-phosphoglycerate-dependent automodification of bacterial enolases implicated in their export?
    Gregory Boel
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG, UMR 2585, F 78850, Thiverval Grignon, France
    J Mol Biol 337:485-96. 2004
    ..The K341E mutant enolase was almost as active as the wild-type enzyme and therefore allowed us to establish that the loss of enolase export correlates with the loss of modification and not the loss of glycolytic activity...
  9. ncbi request reprint How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF
    Ivan Mijakovic
    Microbiologie et Genetique Moleculaire, CNRS INRA INA PG UMR 2585, Thiverval Grignon, France
    J Mol Microbiol Biotechnol 8:19-25. 2004
    ..We propose that this effect could at least partly account for the observed activation of YwqF induced by tyrosine phosphorylation. Potential physiological implications of this finding are discussed...
  10. pmc Pyrophosphate-producing protein dephosphorylation by HPr kinase/phosphorylase: a relic of early life?
    Ivan Mijakovic
    Laboratoire de génétique des microorganismes, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1925, F 78850 Thiverval Grignon, France
    Proc Natl Acad Sci U S A 99:13442-7. 2002
    ..In B. subtilis, this might be achieved by YvoE. This protein exhibits pyrophosphatase activity, and its gene is organized in an operon with hprK...
  11. doi request reprint The mechanisms of carbon catabolite repression in bacteria
    Josef Deutscher
    Laboratorie de Microbiologie et Génétique Moléculaire, INRA AgroParisTech CNRS, F 78850 Thiverval Grignon, France
    Curr Opin Microbiol 11:87-93. 2008
    ..PTS-independent CCR mechanisms are operative in several other bacteria...
  12. pmc Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases
    Ivan Mijakovic
    Laboratoire de génétique des microorganismes, CNRS INRA INA PG UMR2585, 78850 Thiverval Grignon, France
    EMBO J 22:4709-18. 2003
    ..YwqE, which is encoded by the fourth gene of the B.subtilis ywqCDEF operon, also dephosphorylates P-Tyr-YwqD...
  13. pmc Bacillus subtilis mutant LicT antiterminators exhibiting enzyme I- and HPr-independent antitermination affect catabolite repression of the bglPH operon
    Cordula Lindner
    Laboratoire de génétique des microorganismes, INRA CNRS, URA1925, F 78850 Thiverval Grignon, France
    J Bacteriol 184:4819-28. 2002
    ..In agreement with this concept, mutants synthesizing a P approximately His-HPr-independent LicT(Pia) had lost LicT-modulated CCR...
  14. pmc How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria
    Josef Deutscher
    Microbiologie et Genetique Moleculaire, INRA CNRS INA PG UMR 2585, Thiverval Grignon, France
    Microbiol Mol Biol Rev 70:939-1031. 2006
    ..As we shall see, the PTS regulation network not only controls carbohydrate uptake and metabolism but also interferes with the utilization of nitrogen and phosphorus and the virulence of certain pathogens...
  15. ncbi request reprint Antitermination by GlpP, catabolite repression via CcpA and inducer exclusion triggered by P-GlpK dephosphorylation control Bacillus subtilis glpFK expression
    Emmanuelle Darbon
    Laboratoire de génétique des microorganismes, INRA CNRS, URA1925, F 78850 Thiverval Grignon, France
    Mol Microbiol 43:1039-52. 2002
    ..Similarly, despite the presence of glucose, large amounts of glycerol-3-P are formed in a glycerol-exposed strain synthesizing GlpKH230R, as this mutant GlpK is as active as P-GlpK...
  16. pmc Identification and characterization of a fructose phosphotransferase system in Bifidobacterium breve UCC2003
    Alain Mazé
    Department of Microbiology and Alimentary Pharmabiotic Centre, University of Ireland, Cork, Western Road, Cork, Ireland
    Appl Environ Microbiol 73:545-53. 2007
    ..breve is involved in fructose transport and that its expression is controlled by an antiterminator mechanism. Biochemical studies unequivocally demonstrated that FruA phosphorylates fructose at the C-6 position...
  17. ncbi request reprint Structural analysis of the bacterial HPr kinase/phosphorylase V267F mutant gives insights into the allosteric regulation mechanism of this bifunctional enzyme
    Vincent Chaptal
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, 1 Avenue de Terrasse, 91198 Gif sur Yvette, France
    J Biol Chem 282:34952-7. 2007
    ..Together with nucleotide binding fluorescence measurements, these results allowed us to decipher the cooperative behavior of the protein and to gain new insights into the allosteric regulation mechanism of HPrK/P...
  18. ncbi request reprint The cold shock response of Lactobacillus casei: relation between HPr phosphorylation and resistance to freeze/thaw cycles
    Sophie Beaufils
    Laboratoire de Microbiologie de l Environnement EA956 USC INRA 2017, IBFA, Universite de Caen, Caen, France
    J Mol Microbiol Biotechnol 13:65-75. 2007
    ..casei...
  19. ncbi request reprint Maltose transport in Lactobacillus casei and its regulation by inducer exclusion
    Vicente Monedero
    Laboratorio de Bacterias Lacticas y Probioticos, IATA CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
    Res Microbiol 159:94-102. 2008
    ..These results strengthen the idea that P-Ser-HPr controls inducer exclusion and make the maltose system of L. casei a suitable model for studying this process in Firmicutes...
  20. ncbi request reprint Protein-tyrosine phosphorylation in Bacillus subtilis
    Ivan Mijakovic
    Microbial Physiology and Genetics Group, BioCentrum, Technical University of Denmark, Lyngby, Denmark
    J Mol Microbiol Biotechnol 9:189-97. 2005
    ....
  21. ncbi request reprint Dimerization of Crh by reversible 3D domain swapping induces structural adjustments to its monomeric homologue Hpr
    Michel Juy
    Institut de Biologie et Chimie des Proteines, C N R S UMR 5086, 69367 Cedex 07, Lyon, France
    J Mol Biol 332:767-76. 2003
    ..Our data suggest that reversible 3D domain swapping of Crh might be an efficient regulatory mechanism to modulate its activity...
  22. ncbi request reprint Analysis of the elements of catabolite repression in Clostridium acetobutylicum ATCC 824
    Martin Tangney
    School of Life Sciences, Napier University, Edinburgh, UK
    J Mol Microbiol Biotechnol 6:6-11. 2003
    ..Acetobutylicum genome sequence, suggesting that a similar mechanism of carbon catabolite repression may operate in this industrially important organism...
  23. ncbi request reprint Autophosphorylation of the Escherichia coli protein kinase Wzc regulates tyrosine phosphorylation of Ugd, a UDP-glucose dehydrogenase
    Christophe Grangeasse
    Institut de Biologie et Chimie des Proteines, CNRS, Universite de Lyon, 69367 Lyon Cedex 07, France
    J Biol Chem 278:39323-9. 2003
    ....
  24. ncbi request reprint Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation
    Joanne I Yeh
    Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island 02912, USA
    Biochemistry 43:362-73. 2004
    ..On the basis of our structural results, we propose a mechanism by which the phosphorylation of a histidyl residue located 25 A from the active site results in a 10-15-fold increase in the activity of the enterococcal glycerol kinase...
  25. pmc HPr kinase/phosphorylase, the sensor enzyme of catabolite repression in Gram-positive bacteria: structural aspects of the enzyme and the complex with its protein substrate
    Sylvie Nessler
    Laboratoire d Enzymologie et Biochimie Structurales, UPR 9063, CNRS, 91198 Gif sur Yvette, France
    J Bacteriol 185:4003-10. 2003
  26. pmc X-ray structure of a bifunctional protein kinase in complex with its protein substrate HPr
    Sonia Fieulaine
    Laboratoire d Enzymologie et Biochimie Structurales, Unité Propre de Recherche UPR 9063, Centre National de la Recherche Scientifique CNRS, 91198 Gif sur Yvette, France
    Proc Natl Acad Sci U S A 99:13437-41. 2002
    ..This study provides the structure of a protein kinase in complex with its protein substrate, giving insights into the chemistry of the phospho-transfer reactions in both directions...
  27. ncbi request reprint Elaborate transcription regulation of the Bacillus subtilis ilv-leu operon involved in the biosynthesis of branched-chain amino acids through global regulators of CcpA, CodY and TnrA
    Shigeo Tojo
    Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura cho, Fukuyama shi, Hiroshima 729 0292, Japan
    Mol Microbiol 56:1560-73. 2005
    ..The physiological role of this elaborate transcription regulation of the B. subtilis ilv-leu operon in overall metabolic regulation in this organism is discussed...
  28. pmc Structural basis for the regulation mechanism of the tyrosine kinase CapB from Staphylococcus aureus
    Vanesa Olivares-Illana
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS, Gif sur Yvette, France
    PLoS Biol 6:e143. 2008
    ..These observations provide a molecular basis for the regulation mechanism of the bacterial tyrosine kinases and give insights into their copolymerase function...
  29. ncbi request reprint Discovering lactic acid bacteria by genomics
    Todd Klaenhammer
    Department of Food Science, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695 7624, USA
    Antonie Van Leeuwenhoek 82:29-58. 2002
    ..Our collective view of the lactic acid bacteria will be fundamentally changed as we rediscover the relationships and capabilities of these organisms through genomics...
  30. pmc Regulation of Lactobacillus casei sorbitol utilization genes requires DNA-binding transcriptional activator GutR and the conserved protein GutM
    Cristina Alcántara
    Laboratorio de Bacterias Lacticas y Probioticos, IATA CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
    Appl Environ Microbiol 74:5731-40. 2008
    ..A gutM mutant, the first constructed in a firmicute, showed drastically reduced gut operon expression and sorbitol uptake, indicating a regulatory role also for GutM...
  31. pmc Identification of a gene cluster enabling Lactobacillus casei BL23 to utilize myo-inositol
    Maria Jesus Yebra
    Laboratorio de Bacterias Lacticas y Probioticos, IATA CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
    Appl Environ Microbiol 73:3850-8. 2007
    ..This is the first example of an operon for MI utilization in lactic acid bacteria and illustrates the versatility of carbohydrate utilization in L. casei BL23...
  32. ncbi request reprint Tyrosine phosphorylation: an emerging regulatory device of bacterial physiology
    Christophe Grangeasse
    Institute of Biology and Chemistry of Proteins, CNRS, University of Lyon, 7 passage du Vercors, 69367 Lyon, France
    Trends Biochem Sci 32:86-94. 2007
    ....
  33. ncbi request reprint The phosphotransferase system of Lactobacillus casei: regulation of carbon metabolism and connection to cold shock response
    Vicente Monedero
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, Burjassot, Spain
    J Mol Microbiol Biotechnol 12:20-32. 2007
    ..Surprisingly, recent evidence suggests that the PTS of L. casei also plays a role in cold shock response...
  34. ncbi request reprint Solution structure and dynamics of Crh, the Bacillus subtilis catabolite repression HPr
    Adrien Favier
    Jean Pierre Ebel C N R S C E A, Institut de Biologie Structurale, 41 rue Jules Horowitz, 38027 Grenoble Cedex, France
    J Mol Biol 317:131-44. 2002
    ..15)N relaxation measured in the monomeric form also identified differential local mobility in the helix B which is located in the vicinity of this site...
  35. ncbi request reprint Structural analysis of B. subtilis CcpA effector binding site
    Vincent Chaptal
    Laboratoire d Enzymologie et Biochimie Structurales, CNRS FRE 2930, Gif sur Yvette, France
    Proteins 64:814-6. 2006
  36. ncbi request reprint Cloning and characterization of a gene encoding a cold-shock protein in Lactobacillus casei
    Nicolas Sauvageot
    Laboratoire de Microbiologie de l Environnement, Universite de Caen, Esplanade de la Paix, Caen Cedex, France
    FEMS Microbiol Lett 254:55-62. 2006
    ..A cspA mutant strain was constructed and it showed reduced growth rate compared with the wild type at both optimal and low temperatures, demonstrating that CspA plays an important role in the physiology of L. casei...
  37. ncbi request reprint Horizontal gene transfer in the molecular evolution of mannose PTS transporters
    Manuel Zúñiga
    Instituto de Agroquimica y Tecnologia de Alimentos, IATA CSIC, 46100 Burjassot, Valencia, Spain
    Mol Biol Evol 22:1673-85. 2005
    ..The functional role and distribution of mannose-class PTS transporters suggest that their expansion may have played a significant role in the establishment of symbiotic relationships between animals and some bacteria...
  38. pmc In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE
    Ivan Mijakovic
    Microbial Physiology and Genetics Group, BioCentrum, Technical University of Denmark, Lyngby, Denmark
    J Bacteriol 187:3384-90. 2005
    ..In this study, we characterize YwqE, compare the activities of the three B. subtilis PTPs (YwqE, YwlE, and YfkJ), and demonstrate that the two B. subtilis class II PTPs do not dephosphorylate the physiological substrates of YwqE...
  39. ncbi request reprint Molecular analysis of the glucose-specific phosphoenolpyruvate : sugar phosphotransferase system from Lactobacillus casei and its links with the control of sugar metabolism
    María J Yebra
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, Apdo Correos 73, 46100 Burjassot, Spain
    Microbiology 152:95-104. 2006
    ..In addition, the manM mutant exhibited neither inducer exclusion of maltose nor glucose repression. This result confirms the need for glucose transport through the PTS to trigger these regulatory processes in L. casei...
  40. ncbi request reprint The glycolytic genes pfk and pyk from Lactobacillus casei are induced by sugars transported by the phosphoenolpyruvate:sugar phosphotransferase system and repressed by CcpA
    Rosa Viana
    Biotechnology Department, Instituto de Agroquimica y Tecnologia de Alimentos, IATA CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
    Arch Microbiol 183:385-93. 2005
    ..Our results suggest that the need to accommodate the levels of pfk-pyk mRNA to the availability of sugars is fulfilled in L. casei by a PTS/CcpA-mediated signal transduction different from L. lactis...
  41. ncbi request reprint An esterase gene from Lactobacillus casei cotranscribed with genes encoding a phosphoenolpyruvate:sugar phosphotransferase system and regulated by a LevR-like activator and sigma54 factor
    María J Yebra
    Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, Burjassot, Spain
    J Mol Microbiol Biotechnol 8:117-28. 2004
    ....