Patrice Courvalin

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. ncbi request reprint Combinatorial approach of bacteria to antibiotic resistance
    P Courvalin
    Department of Biology, University of California, San Diego, La Jolla 92093 0116, USA
    Res Microbiol 150:367-73. 1999
  2. ncbi request reprint Antibiotic resistance: the pros and cons of probiotics
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Dig Liver Dis 38:S261-5. 2006
  3. pmc Antimicrobial drug resistance: "Prediction is very difficult, especially about the future"
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Emerg Infect Dis 11:1503-6. 2005
  4. ncbi request reprint New plasmid-mediated resistances to antimicrobial agents
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724, Paris Cedex 15, France
    Arch Microbiol 189:289-91. 2008
  5. doi request reprint Can pharmacokinetic-pharmacodynamic parameters provide dosing regimens that are less vulnerable to resistance?
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Microbiol Infect 14:989-94. 2008
  6. pmc VanD-type vancomycin-resistant Enterococcus faecium and Enterococcus faecalis
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 48:3892-904. 2004
  7. ncbi request reprint Minimizing potential resistance: the molecular view
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Infect Dis 33:S138-46. 2001
  8. ncbi request reprint Genetics of glycopeptide resistance in gram-positive pathogens
    Patrice Courvalin
    Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Int J Med Microbiol 294:479-86. 2005
  9. ncbi request reprint Vancomycin resistance in gram-positive cocci
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Infect Dis 42:S25-34. 2006
  10. doi request reprint Predictable and unpredictable evolution of antibiotic resistance
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Intern Med 264:4-16. 2008

Detail Information

Publications128 found, 100 shown here

  1. ncbi request reprint Combinatorial approach of bacteria to antibiotic resistance
    P Courvalin
    Department of Biology, University of California, San Diego, La Jolla 92093 0116, USA
    Res Microbiol 150:367-73. 1999
    ..The data provided are mainly, if not exclusively, taken from the work carried out in the laboratory, although there are numerous other examples in the literature...
  2. ncbi request reprint Antibiotic resistance: the pros and cons of probiotics
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Dig Liver Dis 38:S261-5. 2006
    ..This article reviews the experiments to be performed and the criteria for assessment of safety of antibiotic resistant probiotics...
  3. pmc Antimicrobial drug resistance: "Prediction is very difficult, especially about the future"
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Emerg Infect Dis 11:1503-6. 2005
    ..The 2 phenomena are not mutually exclusive and can be associated in the emergence and more efficient spread of resistance. This review discusses the predictable future of the relationship between antimicrobial drugs and bacteria...
  4. ncbi request reprint New plasmid-mediated resistances to antimicrobial agents
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724, Paris Cedex 15, France
    Arch Microbiol 189:289-91. 2008
  5. doi request reprint Can pharmacokinetic-pharmacodynamic parameters provide dosing regimens that are less vulnerable to resistance?
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Microbiol Infect 14:989-94. 2008
    ..In this review, the pharmacokinetic-pharmacodynamic (PK-PD) parameters that are intended to lower resistance dissemination are considered exclusively...
  6. pmc VanD-type vancomycin-resistant Enterococcus faecium and Enterococcus faecalis
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 48:3892-904. 2004
    ....
  7. ncbi request reprint Minimizing potential resistance: the molecular view
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Infect Dis 33:S138-46. 2001
    ....
  8. ncbi request reprint Genetics of glycopeptide resistance in gram-positive pathogens
    Patrice Courvalin
    Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA
    Int J Med Microbiol 294:479-86. 2005
    ..Importantly, it has been recently acquired by Staphylococcus aureus. We shall review the genetics which accounts for this successful dissemination and discuss the possible origins of the resistance genes...
  9. ncbi request reprint Vancomycin resistance in gram-positive cocci
    Patrice Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Infect Dis 42:S25-34. 2006
    ..The diversity, regulation, evolution, and recent dissemination of methicillin-resistant Staphylococcus aureus are then discussed...
  10. doi request reprint Predictable and unpredictable evolution of antibiotic resistance
    P Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Intern Med 264:4-16. 2008
    ..In this review, we will consider the predictable future of the relationship between bacteria and antibiotics...
  11. ncbi request reprint The vanG glycopeptide resistance operon from Enterococcus faecalis revisited
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 50:931-48. 2003
    ..Sequence determination of the flanking regions of the vanG cluster in donor and transconjugants revealed the same 4 bp direct repeats and 22 bp imperfect inverted repeats that delineated the large element...
  12. pmc Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA-mediated efflux in Escherichia coli
    Bruno Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 51:2464-9. 2007
    ..The transporter conferred resistance to the hydrophilic quinolones norfloxacin and ciprofloxacin...
  13. pmc Sequence of conjugative plasmid pIP1206 mediating resistance to aminoglycosides by 16S rRNA methylation and to hydrophilic fluoroquinolones by efflux
    Bruno Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 52:2581-92. 2008
    ....
  14. pmc Regulation of VanB-type vancomycin resistance gene expression by the VanS(B)-VanR (B) two-component regulatory system in Enterococcus faecalis V583
    S Evers
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique EP J0058, Institut Pasteur, Paris, France
    J Bacteriol 178:1302-9. 1996
    ..Vancomycin, but not teicoplanin, was an inducer, which explains teicoplanin susceptibility of VanB-type enterococci...
  15. ncbi request reprint Expression of glycopeptide-resistance gene in response to vancomycin and teicoplanin in the cardiac vegetations of rabbits infected with VanB-type Enterococcus faecalis
    Agnes Lefort
    Institut National de la Sante et de la Recherche Medicale, Equipe Mixté INSERM Université 9933, Paris, France
    J Infect Dis 189:90-7. 2004
    ..Teicoplanin-resistant mutants were detected as fluorescent bacteria in rabbits treated with teicoplanin. Thus, the reporter system monitored expression of a glycopeptide-resistance gene in vivo at a single-cell level...
  16. pmc Heterologous expression of the enterococcal vanA operon in methicillin-resistant Staphylococcus aureus
    Bruno Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 48:4281-5. 2004
    ..aureus PA-VRSA is thus likely due to instability of the genetic element, plasmid or transposon, carrying the vanA operon associated with a longer lag phase before growth resumes after induction by vancomycin...
  17. pmc Synergism between beta-lactams and glycopeptides against VanA-type methicillin-resistant Staphylococcus aureus and heterologous expression of the vanA operon
    Bruno Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 50:3622-30. 2006
    ..These observations indicate that cell wall inhibitors of the beta-lactam and glycopeptide classes exert strong and mutual antagonistic effects on resistance to each other against VanA-type methicillin-resistant S. aureus...
  18. pmc Molecular basis of vancomycin dependence in VanA-type Staphylococcus aureus VRSA-9
    Djalal Meziane-Cherif
    Institut Pasteur, Unite des Agents Antibacteriens, CNRS URA 2185, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 192:5465-71. 2010
    ..Ddl drug targeting could therefore be an effective strategy against vancomycin-resistant S. aureus...
  19. pmc Staphylococcus aureus VRSA-11B is a constitutive vancomycin-resistant mutant of vancomycin-dependent VRSA-11A
    Bruno Perichon
    Institut Pasteur, Unite des Agents Antibacteriens, Paris, France
    Antimicrob Agents Chemother 56:4693-6. 2012
    ..Synthesis of peptidoglycan precursors ending in d-Ala-d-lactate was responsible for oxacillin susceptibility of VRSA-11A and VRSA-11B despite the presence of a wild-type mecA gene in both strains...
  20. pmc VanE-type vancomycin-resistant Enterococcus faecalis clinical isolates from Australia
    Lorena Abadía-Patiño
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 48:4882-5. 2004
    ..Transcription analysis of the vanE operons suggested that the five genes were cotranscribed from an initiation site located 25 bp upstream from the ATG start codon of vanE...
  21. ncbi request reprint A six amino acid deletion, partially overlapping the VanSB G2 ATP-binding motif, leads to constitutive glycopeptide resistance in VanB-type Enterococcus faecium
    Florence Depardieu
    Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France
    Mol Microbiol 50:1069-83. 2003
    ..We suggest that the heptanucleotide direct repeat in vanSB may favour the appearance of high level constitutively expressed vancomycin resistance through a 'slippage' type of genetic rearrangement in VanB-type strains...
  22. pmc vanE gene cluster of vancomycin-resistant Enterococcus faecalis BM4405
    Lorena Abadía Patiño
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 184:6457-64. 2002
    ..The vanE, vanXYE, and vanTE genes conferred inducible low-level resistance to vancomycin after cloning in E. faecalis JH2-2, probably following cross talk with a two-component regulatory system of the host...
  23. pmc VanA-type Staphylococcus aureus strain VRSA-7 is partially dependent on vancomycin for growth
    Carole Moubareck
    Institut Pasteur, Unite des Agents Antibacteriens, Centre National de Référence de la Résistance aux Antibiotiques, Paris Cedex 15 75724, France
    Antimicrob Agents Chemother 53:3657-63. 2009
    ..Since peptidoglycan precursors ending in D-Ala-D-Lac are not processed by PBP 2', the strain was fully susceptible to oxacillin, despite the production of a wild-type PBP 2'...
  24. pmc AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii
    Laurence Damier-Piolle
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 52:557-62. 2008
    ..The chemical structure of these molecules suggests that amphiphilic compounds are the preferred substrates. The AdeABC and AdeIJK efflux systems contributed in a more than additive fashion to tigecycline resistance...
  25. pmc Characterization of the vanD glycopeptide resistance gene cluster from Enterococcus faecium BM4339
    B Casadewall
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    J Bacteriol 181:3644-8. 1999
    ..However, since expression of the resistance genes is constitutive, growth of E. faecium BM4339 was not dependent on the presence of glycopeptides in the culture medium...
  26. pmc Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis
    Djalal Meziane-Cherif
    Institut Pasteur, Unite des Agents Antibacteriens, Paris Cedex 15, France
    J Biol Chem 287:37583-92. 2012
    ..This study provides additional insight into the molecular evolution of d-Ala:d-X ligases and could contribute to the development of new structure-based inhibitors of vancomycin resistance enzymes...
  27. pmc Inducible expression eliminates the fitness cost of vancomycin resistance in enterococci
    Marie Laure Foucault
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 107:16964-9. 2010
    ..Our findings are in agreement with the observation that regulation of expression is common in horizontally acquired resistance and represents an efficient evolutionary pathway for resistance determinants to become selectively neutral...
  28. pmc Genetic and biochemical characterization of OXA-63, a new class D beta-lactamase from Brachyspira pilosicoli BM4442
    Djalal Meziane-Cherif
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 52:1264-8. 2008
    ..16 microM) and exhibited low susceptibility to NaCl (IC50 of >2 M). OXA-63 is the first DBL described for the anaerobic spirochete B. pilosicoli...
  29. pmc Glycopeptide resistance vanA operons in Paenibacillus strains isolated from soil
    Luca Guardabassi
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 49:4227-33. 2005
    ..Altogether, the results are compatible with the notion that van operons in soil Paenibacillus strains and in enterococci have evolved from a common ancestor...
  30. pmc VanB-type Enterococcus faecium clinical isolate successively inducibly resistant to, dependent on, and constitutively resistant to vancomycin
    Alvaro San Millan
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 53:1974-82. 2009
    ..Instead, a mutation in the transcription terminator of the regulatory genes resulted in transcriptional readthrough of the resistance genes from the P(RB) promoter in the absence of vancomycin...
  31. ncbi request reprint Characterization of transposon Tn1549, conferring VanB-type resistance in Enterococcus spp
    F Garnier
    Unité des Agents Antibactériens and Laboratoire de Génomique des Micro organismes Pathogènes, Institut Pasteur, 75724 Paris Cedex 15, France
    Microbiology 146:1481-9. 2000
    ....
  32. pmc Influence of VanD type resistance on activities of glycopeptides in vitro and in experimental endocarditis due to Enterococcus faecium
    Agnes Lefort
    Institut National de la Sante et de la Recherche Medicale, EMI U 9933, Hôpital Bichat Claude Bernard Unité des Agents Antibactériens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 47:3515-8. 2003
    ..These results suggest that detection of VanD type resistance is of major importance because it abolishes in vivo glycopeptide activity and allows the emergence of mutants highly resistant to glycopeptides...
  33. pmc Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation
    Marc Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 47:2565-71. 2003
    ..It therefore appears that posttranscriptional modification of 16S rRNA can confer high-level broad-range resistance to aminoglycosides in gram-negative human pathogens...
  34. doi request reprint Organic and conventional fruits and vegetables contain equivalent counts of Gram-negative bacteria expressing resistance to antibacterial agents
    Raymond Ruimy
    EA 6934 lt lt Résistance Bactérienne in vivo gt gt Université Paris Diderot, and Centre National de Référence sur la Résistance aux Antibiotiques, Hopital Bichat Claude Bernard, APHP, Paris, France
    Environ Microbiol 12:608-15. 2010
    ..which grouped into two phylotypes and all carrying the bla(RAHN) gene. Thus, both organic and conventional fruits and vegetables may constitute significant sources of resistant bacteria and of resistance genes...
  35. pmc GES-11, a novel integron-associated GES variant in Acinetobacter baumannii
    Carole Moubareck
    Institut Pasteur, Unite des Agents Antibacteriens, Centre National de Référence de la Résistance aux Antibiotiques, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 53:3579-81. 2009
    ..The structural gene was part of a class 1 integron borne by self-transferable plasmid pIP847. GES-type beta-lactamases have not been reported previously in A. baumannii...
  36. pmc Genetic and biochemical characterization of CAD-1, a chromosomally encoded new class A penicillinase from Carnobacterium divergens
    Djalal Meziane-Cherif
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 52:551-6. 2008
    ..5 microM). The bla(CAD-1) gene is likely to have been acquired by BM4489 and BM4490 as part of a mobile genetic element, since it was not found in the susceptible type strain CIP 101029 and was adjacent to a gene for a resolvase...
  37. pmc Regulation of expression of the vanD glycopeptide resistance gene cluster from Enterococcus faecium BM4339
    B Casadewall
    , Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 183:3436-46. 2001
    ..This activity is not required for glycopeptide resistance in BM4339, since this strain cannot synthesize D-alanyl-D-alanine...
  38. pmc New combinations of mutations in VanD-Type vancomycin-resistant Enterococcus faecium, Enterococcus faecalis, and Enterococcus avium strains
    F Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 53:1952-63. 2009
    ..Based on sequence differences, the vanD gene clusters could be assigned to two subtypes: vanD-1 and vanD-4...
  39. pmc Silencing of glycopeptide resistance in Enterococcus faecalis BM4405 by novobiocin
    Lorena Abadía Patiño
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 49:1419-25. 2005
    ..Taken together, these data suggest that alteration of DNA supercoiling following a mutation in GyrB was responsible for lack of expression of the vanE operon and thus for vancomycin susceptibility in BM4405-1...
  40. pmc Expression of the resistance-nodulation-cell division pump AdeIJK in Acinetobacter baumannii is regulated by AdeN, a TetR-type regulator
    Nicolas Rosenfeld
    Institut Pasteur, Unite des Agents Antibacteriens, Genopole Plate Forme Génomique, Paris, France
    Antimicrob Agents Chemother 56:2504-10. 2012
    ..The adeN gene was detected in all of the 30 A. baumannii strains tested and in Acinetobacter calcoaceticus, Acinetobacter nosocomialis, and Acinetobacter pittii...
  41. ncbi request reprint Binding sites of VanRB and sigma70 RNA polymerase in the vanB vancomycin resistance operon of Enterococcus faecium BM4524
    Florence Depardieu
    Unite des Agents Antibacteriens, URA CNRS 2172, Institut Pasteur, 75724 Paris, Cedex 15, France
    Mol Microbiol 57:550-64. 2005
    ....
  42. pmc Expression of the RND-type efflux pump AdeABC in Acinetobacter baumannii is regulated by the AdeRS two-component system
    Isabelle Marchand
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 48:3298-304. 2004
    ..These mutations led to constitutive expression of the pump and, thus, to antibiotic resistance. These data indicate that the AdeABC pump is cryptic in wild A. baumannii due to stringent control by the AdeRS two-component system...
  43. pmc Detection of the van alphabet and identification of enterococci and staphylococci at the species level by multiplex PCR
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Clin Microbiol 42:5857-60. 2004
    ..This specific and sensitive technique allows detection of glycopeptide-resistant strains, in particular methicillin-resistant S. aureus, that may escape phenotype-based automated rapid methods...
  44. pmc Activation of the cryptic aac(6')-Iy aminoglycoside resistance gene of Salmonella by a chromosomal deletion generating a transcriptional fusion
    S Magnet
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Bacteriol 181:6650-5. 1999
    ..This genomic environment suggests a role in the catabolism of a specific sugar for AAC(6')-Iy in Salmonella...
  45. ncbi request reprint Sequence of the vanB and ddl genes encoding D-alanine:D-lactate and D-alanine:D-alanine ligases in vancomycin-resistant Enterococcus faecalis V583
    S Evers
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique EP J0058, Institut Pasteur, Paris, France
    Gene 140:97-102. 1994
    ..After induction of VmR, En. faecalis V583 synthesized the cell wall precursor, UDP-N-acetylmuramyl-tetrapeptide-D-lactate, indicating that the mechanism of glycopeptide resistance in strains with the VanA and VanB phenotype is similar...
  46. pmc Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454
    S Magnet
    , Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 45:3375-80. 2001
    ..The products of two upstream open reading frames encoding a putative two-component regulatory system might be involved in the regulation of expression of the adeABC gene cluster...
  47. ncbi request reprint Characterization of the plasmid genes blaT-4 and blaT-5 which encode the broad-spectrum beta-lactamases TEM-4 and TEM-5 in enterobacteriaceae
    W Sougakoff
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Gene 78:339-48. 1989
    ....
  48. ncbi request reprint Aminoglycoside resistance by ArmA-mediated ribosomal 16S methylation in human bacterial pathogens
    Grace F Liou
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    J Mol Biol 359:358-64. 2006
    ....
  49. pmc VanA-type vancomycin-resistant Staphylococcus aureus
    Bruno Perichon
    Institut Pasteur, Unite des Agents Antibacteriens, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 53:4580-7. 2009
    ..The association of multiple molecular and environmental factors has been implicated in the regional emergence of VRSA in Michigan...
  50. pmc Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray
    Sébastien Coyne
    Institut Pasteur, Unite des Agents Antibacteriens, 75724 Paris, Cedex 15, France
    Antimicrob Agents Chemother 54:333-40. 2010
    ..The microarray is potentially useful for detection of resistance in A. baumannii and should allow detection of new efflux systems associated with antibiotic resistance...
  51. pmc Intrinsic resistance to aminoglycosides in Enterococcus faecium is conferred by the 16S rRNA m5C1404-specific methyltransferase EfmM
    Marc Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, F 75724 Paris Cedex 15, France
    RNA 17:251-62. 2011
    ....
  52. pmc Contribution of mutations in gyrA and parC genes to fluoroquinolone resistance of mutants of Streptococcus pneumoniae obtained in vivo and in vitro
    J Tankovic
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique EP J0058, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 40:2505-10. 1996
    ..These data strongly suggest that ParC is the primary target for fluoroquinolones in S. pneumoniae and that BM4205-R3 is resistant to higher levels of the drugs following the acquisition of two mutations, including one in gyrA...
  53. ncbi request reprint The vanB gene of vancomycin-resistant Enterococcus faecalis V583 is structurally related to genes encoding D-Ala:D-Ala ligases and glycopeptide-resistance proteins VanA and VanC
    S Evers
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Gene 124:143-4. 1993
    ..faecalis V583. The DNA fragment hybridized to VmR strains of En. faecium and En. faecalis, but not to their susceptible derivatives...
  54. pmc RmtF, a new member of the aminoglycoside resistance 16S rRNA N7 G1405 methyltransferase family
    Marc Galimand
    Institut Pasteur, Unite des Agents Antibacteriens, Paris, France
    Antimicrob Agents Chemother 56:3960-2. 2012
    ..The deduced RmtF protein had 25 to 46% identity with members of the N7 G1405 family of aminoglycoside resistance 16S rRNA methyltransferases...
  55. pmc Efflux pump Lde is associated with fluoroquinolone resistance in Listeria monocytogenes
    Sylvain Godreuil
    Unité des Agents Antibactériens Laboratoire des Listeria, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 47:704-8. 2003
    ....
  56. pmc Modes and modulations of antibiotic resistance gene expression
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Clin Microbiol Rev 20:79-114. 2007
    ..We will review certain mechanisms, all reversible, that bacteria have elaborated to achieve antibiotic resistance by the fine-tuning of the expression of genetic information...
  57. doi request reprint Acquisition of multidrug resistance transposon Tn6061 and IS6100-mediated large chromosomal inversions in Pseudomonas aeruginosa clinical isolates
    Sébastien Coyne
    Institut Pasteur, Unite des Agents Antibacteriens, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 156:1448-58. 2010
    ..Acquisition of Tn6061 and chromosomal inversions are further examples of intricate mechanisms that contribute to the genome plasticity of P. aeruginosa...
  58. pmc Distribution of extended-spectrum beta-lactamases in clinical isolates of Enterobacteriaceae in Vietnam
    Van Cao
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 46:3739-43. 2002
    ..Molecular typing of the strains with a similar resistance phenotype to broad-spectrum cephalosporins indicated polyclonal spread. ISEcp1 was presumably responsible for dissemination of the bla(CTX-M-like) gene...
  59. ncbi request reprint A new example of physical linkage between Tn1 and Tn21: the antibiotic multiple-resistance region of plasmid pCFF04 encoding extended-spectrum beta-lactamase TEM-3
    C Mabilat
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Mol Gen Genet 235:113-21. 1992
    ..The presence of an integron can account for the various assortments of aminoglycoside resistance genes found associated with blaTEM-3...
  60. ncbi request reprint Single-cell analysis of glycopeptide resistance gene expression in teicoplanin-resistant mutants of a VanB-type Enterococcus faecalis
    M Baptista
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Mol Microbiol 32:17-28. 1999
    ..Transglycosylase inhibition was probably the stimulus for the heterologous kinase, as moenomycin was also an inducer...
  61. pmc RND-type efflux pumps in multidrug-resistant clinical isolates of Acinetobacter baumannii: major role for AdeABC overexpression and AdeRS mutations
    Eun Jeong Yoon
    Institut Pasteur, Unite des Agents Antibacteriens, Paris, France
    Antimicrob Agents Chemother 57:2989-95. 2013
    ..This report outlines the high incidence of AdeABC efflux pump overexpression in MDR A. baumannii as a result of a variety of single mutations in the corresponding two-component regulatory system...
  62. pmc Overexpression of resistance-nodulation-cell division pump AdeFGH confers multidrug resistance in Acinetobacter baumannii
    Sébastien Coyne
    Institut Pasteur, Unite des Agents Antibacteriens, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 54:4389-93. 2010
    ..Mutations in adeL were found in the three adeFGH-overexpressing mutants, suggesting that they were responsible for overexpression of AdeFGH...
  63. ncbi request reprint Emergence and dissemination of a new mechanism of resistance to aminoglycosides in Gram-negative bacteria: 16S rRNA methylation
    Marc Galimand
    Unité des Agents Antibactériens and Centre National de Référence de la Résistance aux Antibiotiques, Institut Pasteur, Paris, France
    Euro Surveill 10:E050127.2. 2005
  64. pmc VanD-type vancomycin-resistant Enterococcus faecium 10/96A
    Florence Depardieu
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris, Cedex 15, France
    Antimicrob Agents Chemother 47:7-18. 2003
    ..This new insertion belonged to the IS605 family and was composed of two open reading frames encoding putative transposases of two unrelated insertion sequence elements, IS200 and IS1341...
  65. ncbi request reprint Eukaryotic promoters can direct protein synthesis in Gram-negative bacteria
    Sylvie Goussard
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    J Mol Microbiol Biotechnol 6:211-8. 2003
    ..These observations should incite to exert caution when interpreting data on the DNA transfer from bacteria to mammallian cells when the genes of interest are under the control of the PCMV or PRSV promoter...
  66. pmc Fitness cost of VanA-type vancomycin resistance in methicillin-resistant Staphylococcus aureus
    Marie Laure Foucault
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 53:2354-9. 2009
    ..Thus, the potential for the dissemination of VRSA clinical isolates should not be underestimated...
  67. ncbi request reprint Resistance phenotypes conferred by macrolide phosphotransferases
    Olivier Chesneau
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    FEMS Microbiol Lett 269:317-22. 2007
    ..The mph(C) gene, as reported for mph(B), also conferred resistance to spiramycin. The mph(A) gene was unique in conferring resistance to azithromycin. The four investigated genes conferred resistance to telithromycin...
  68. pmc Crystallization and preliminary X-ray analysis of a D-Ala:D-Ser ligase associated with VanG-type vancomycin resistance
    Patrick Weber
    Institut Pasteur, Plateforme 6, CNRS URA 2185, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1024-6. 2009
    ..1, c = 177.2 A, and contained two molecules in the asymmetric unit. A complete data set has been collected to 2.35 A resolution from a single crystal under cryogenic conditions using synchrotron radiation...
  69. ncbi request reprint An IS1-like element is responsible for high-level synthesis of extended-spectrum beta-lactamase TEM-6 in Enterobacteriaceae
    S Goussard
    Unite des Agents Antibacteriens, CNRS UA 271, Institut Pasteur, Paris, France
    J Gen Microbiol 137:2681-7. 1991
    ..The element appeared to be widespread and was detected in strains producing TEM-6 or other TEM variants...
  70. pmc TEM-103/IRT-28 beta-lactamase, a new TEM variant produced by Escherichia coli BM4511
    Rodrigo Alonso
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 46:3627-9. 2002
    ..Resistance was due to production of a new TEM-type beta-lactamase, designated TEM-103/IRT-28, characterized by the Arg(275)Leu substitution and encoded by the ca. 62-kb pIP845 conjugative plasmid of the IncI1 incompatibility group...
  71. pmc Aminoglycoside resistance gene ant(4')-IIb of Pseudomonas aeruginosa BM4492, a clinical isolate from Bulgaria
    Stefana Sabtcheva
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 47:1584-8. 2003
    ..aeruginosa tested and was plasmid borne in the remaining strain. The ant(4')-IIb gene was detected by PCR in some clinical strains of P. aeruginosa from the same hospital but not in members of other bacterial genera...
  72. pmc Consequences of VanE-type resistance on efficacy of glycopeptides in vitro and in experimental endocarditis due to Enterococcus faecalis
    M Lafaurie
    , EMI 9933, , Paris, France
    Antimicrob Agents Chemother 45:2826-30. 2001
    ..In conclusion, the use of a high dose of vancomycin was necessary for the treatment of experimental enterococcal endocarditis due to VanE-type strains...
  73. pmc Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France
    A Morvan
    Institut Pasteur, Centre National de Référence de la Résistance aux Antibiotiques, Paris, France
    Antimicrob Agents Chemother 54:2728-31. 2010
    ....
  74. pmc Nucleotide sequence of the erythromycin resistance gene of the conjugative transposon Tn1545
    P Trieu-Cuot
    Unite des Agents Antibacteriens, CNRS UA 271, Institut Pasteur, Paris, France
    Nucleic Acids Res 18:3660. 1990
  75. ncbi request reprint Association between the vanB2 glycopeptide resistance operon and Tn1549 in enterococci from France
    A Umeda
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Antimicrob Chemother 50:253-6. 2002
    ..Linkage between the vanB2 gene cluster and transposon Tn1549 was found in clonally unrelated VanB-type vancomycin-resistant Enterococcus spp. strains isolated in France. The transposon was chromosomally located or plasmid borne...
  76. pmc Efflux-mediated antibiotic resistance in Acinetobacter spp
    Sébastien Coyne
    Institut Pasteur, Unite des Agents Antibacteriens, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 55:947-53. 2011
    ..have been detected and are mainly encoded by mobile genetic elements...
  77. pmc Chromosomal aadD2 encodes an aminoglycoside nucleotidyltransferase in Bacillus clausii
    Bulent Bozdogan
    Service de Microbiologie, CHU Cote de Nacre, Caen Unité des Agents Antibactériens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 47:1343-6. 2003
    ..The aadD2 gene was chromosomally located in all strains and was not transferable by conjugation. These data indicate that chromosomal aadD2 is specific to B. clausii...
  78. pmc Bacterial and host factors implicated in nasal carriage of methicillin-resistant Staphylococcus aureus in mice
    Bruno Gonzalez-Zorn
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 73:1847-51. 2005
    ..aureus strains and demonstrated increased colonization levels by MRSA in cystic fibrosis transmembrane conductance regulator-deficient mice and Toll-like receptor 2 (TLR2)-deficient mice but not TLR4-deficient mice...
  79. pmc Characterization of the chromosomal aac(6')-Iz gene of Stenotrophomonas maltophilia
    T Lambert
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 43:2366-71. 1999
    ..The MICs of gentamicin against these strains of S. maltophilia were lower than those of amikacin, netilmicin, and tobramycin, indicating that production of AAC(6')-Iz contributes to aminoglycoside resistance in S. maltophilia...
  80. pmc Characterization of a mutation in the parE gene that confers fluoroquinolone resistance in Streptococcus pneumoniae
    B Perichon
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique, EPJ 0058, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 41:1166-7. 1997
    ..Sequential acquisition of mutations in parE and gyrA leads to higher levels of resistance. This confirms that topoisomerase IV is the primary target of fluoroquinolones in S. pneumoniae...
  81. pmc Glycopeptide-resistant Enterococcus faecium BM4416 is a VanD-type strain with an impaired D-Alanine:D-Alanine ligase
    B Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 44:1346-8. 2000
    ..faecium BM4339, the only VanD-type strain described so far, BM4416 produced an impaired D-alanine:D-alanine ligase. Unlike for BM4339, which had a 5-bp insertion in ddl, inactivation of the gene in BM4416 was due to insertion of IS19...
  82. pmc Spectinomycin resistance in Neisseria spp. due to mutations in 16S rRNA
    M Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 44:1365-6. 2000
    ..Spectinomycin resistance in clinical isolates of Neisseria meningitidis and Neisseria gonorrhoeae was found to be due to mutations G1064C and C1192U (Escherichia coli numbering) in 16S rRNA genes, respectively...
  83. ncbi request reprint Functional gene transfer from intracellular bacteria to mammalian cells
    C Grillot-Courvalin
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Nat Biotechnol 16:862-6. 1998
    ..Expression of the acquired genes occurs in both dividing and quiescent cells. The only requirement for bacteria to transfer genetic material into nonprofessional phagocytic cells and macrophages is the ability to invade the host cell...
  84. ncbi request reprint Kinetic and mutagenic characterization of the chromosomally encoded Salmonella enterica AAC(6')-Iy aminoglycoside N-acetyltransferase
    S Magnet
    Unite des Agents Antibacteriens, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Biochemistry 40:3700-9. 2001
    ..Solvent kinetic isotope effects on V/K were normal for the WT enzyme and inverse for the double mutant. We discuss a chemical mechanism and the likely rate-limiting steps for both the wild-type and mutant forms of the enzyme...
  85. pmc VanD-type glycopeptide-resistant Enterococcus faecium BM4339
    B Perichon
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 41:2016-8. 1997
    ..This new type of glycopeptide resistance phenotype was designated VanD...
  86. pmc The VanS sensor negatively controls VanR-mediated transcriptional activation of glycopeptide resistance genes of Tn1546 and related elements in the absence of induction
    M Arthur
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique EP J0058, Institut Pasteur, Paris, France
    J Bacteriol 179:97-106. 1997
    ..Activation of the VanR response regulator in the absence of VanS may involve autophosphorylation of VanR with acetyl phosphate or phosphorylation by a heterologous histidine protein kinase...
  87. ncbi request reprint Characterization of the sat4 gene encoding a streptothricin acetyltransferase in Campylobacter coli BE/G4
    J Jacob
    Unite des Agents Antibacteriens, CNRS, Institut Pasteur, Paris, France
    FEMS Microbiol Lett 120:13-7. 1994
    ..coli and streptothricin producers Streptomyces lavendulae and Streptomyces noursei. The sat4 gene was detected by hybridization in clinical and environmental isolates of Campylobacter spp...
  88. ncbi request reprint Glycopeptide resistance in enterococci
    M Arthur
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique, EP J0058, Institut Pasteur, Paris, France
    Trends Microbiol 4:401-7. 1996
    ....
  89. pmc Updated sequence information for TEM beta-lactamase genes
    S Goussard
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 43:367-70. 1999
    ..The sequences of the promoter regions and of the structural genes for 13 penicillinase, extended-spectrum, and inhibitor-resistant TEM-type beta-lactamases have been determined, and an updated blaTEM gene nomenclature is proposed...
  90. ncbi request reprint Quantitative analysis of the metabolism of soluble cytoplasmic peptidoglycan precursors of glycopeptide-resistant enterococci
    M Arthur
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique EP J0058, Institut Pasteur, Paris, France
    Mol Microbiol 21:33-44. 1996
    ..Differences at the regulatory level accounted for phenotypic diversity in acquired glycopeptide resistance by production of D-lac-ending precursors...
  91. pmc Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548
    M Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 49:2949-53. 2005
    ..It thus appears that posttranscriptional modification of 16S rRNA confers high-level resistance to all the clinically available aminoglycosides except streptomycin in gram-negative human and animal pathogens...
  92. ncbi request reprint Evaluation of non-automated techniques for phenotypic detection of VanA-type Staphylococcus aureus
    C Girard-Blanc
    Centre National de Référence de la Résistance aux Antibiotiques and Unité des Agents Antibactériens, Institut Pasteur, 25 rue du Docteur Roux, 75724, Paris Cedex 15, France
    Eur J Clin Microbiol Infect Dis 24:562-5. 2005
    ..In each of these methods, vancomycin was more efficient than teicoplanin for detecting glycopeptide resistance...
  93. pmc High-level aminoglycoside resistance in the beta-hemolytic group G Streptococcus isolate BM2721
    M Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 43:3008-10. 1999
    ..The corresponding genes were found to be adjacent as the result of a recombination event between Tn4001 and Tn5405, two transposons originating in staphylococci...
  94. ncbi request reprint Inactivation of lincosaminide antibiotics in Staphylococcus. Identification of lincosaminide O-nucleotidyltransferases and comparison of the corresponding resistance genes
    A Brisson-Noel
    Unite des Agents Antibacteriens, Centre National de la Recherche Scientifique UA 271, Institut Pasteur, Paris, France
    J Biol Chem 263:15880-7. 1988
    ..haemolyticus BM4610. The two enzymes are 161 amino acids long and differ by 14 amino acid substitutions...
  95. ncbi request reprint Requirement of the VanY and VanX D,D-peptidases for glycopeptide resistance in enterococci
    M Arthur
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Mol Microbiol 30:819-30. 1998
    ..Thus, VanX and VanY had non-overlapping functions involving the hydrolysis of D-Ala-D-Ala and the removal of D-Ala from membrane-bound lipid intermediates respectively...
  96. pmc ColE1-like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum beta-lactamase CTX-M-17
    Van Cao
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 46:1212-7. 2002
    ..Results of primer extension experiments indicated that ISEcp1 provides the promoter for expression of bla(CTX-M-17) and may contribute to dissemination of this gene...
  97. pmc Integron-associated antibiotic resistance and phylogenetic grouping of Escherichia coli isolates from healthy subjects free of recent antibiotic exposure
    David Skurnik
    Université Paris 7 and Groupe Hospitalier Bichat Claude Bernard, AP HP, Paris, France
    Antimicrob Agents Chemother 49:3062-5. 2005
    ....
  98. pmc Resistance of Yersinia pestis to antimicrobial agents
    Marc Galimand
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 50:3233-6. 2006
  99. ncbi request reprint Human infections caused by glycopeptide-resistant Enterococcus spp: are they a zoonosis?
    A Sundsfjord
    Unite des Agents Antibacteriens, Institut Pasteur, Paris, France
    Clin Microbiol Infect 7:16-33. 2001
    ....
  100. pmc Bacterial resistance to penicillin G by decreased affinity of penicillin-binding proteins: a mathematical model
    L Temime
    Institut National de la Sante et de la Recherche Medicale, Unité 444, Paris, France
    Emerg Infect Dis 9:411-7. 2003
    ..pneumoniae and N. meningitidis...
  101. pmc Characterization of In40 of Enterobacter aerogenes BM2688, a class 1 integron with two new gene cassettes, cmlA2 and qacF
    M C Ploy
    Unite des Agents Antibacteriens, Institut Pasteur, 75724 Paris Cedex 15, France
    Antimicrob Agents Chemother 42:2557-63. 1998
    ..The occurrence of this DNA rearrangement, which did not involve homologous sequences, suggests that the In40 integrase could promote recombination at secondary sites...