S T Cole

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. ncbi request reprint Learning from the genome sequence of Mycobacterium tuberculosis H37Rv
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    FEBS Lett 452:7-10. 1999
  2. ncbi request reprint Massive gene decay in the leprosy bacillus
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Nature 409:1007-11. 2001
  3. ncbi request reprint Why sequence the genome of Mycobacterium tuberculosis?
    S T Cole
    Unité de Génétique Moléculaire, Bactérienne, Institut Pasteur, Paris, France
    Tuber Lung Dis 77:486-90. 1996
  4. ncbi request reprint Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
    S T Cole
    Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
    Nature 393:537-44. 1998
  5. ncbi request reprint Repetitive sequences in Mycobacterium leprae and their impact on genome plasticity
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, France
    Lepr Rev 72:449-61. 2001
  6. ncbi request reprint Comparative mycobacterial genomics
    S T Cole
    Unité de Génétique Moléculaire Bactérienne Institut Pasteur 28 rue du Docteur Roux 75724 Paris Cedex 15 France
    Curr Opin Microbiol 1:567-71. 1998
  7. ncbi request reprint Comparative mycobacterial genomics as a tool for drug target and antigen discovery
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Eur Respir J Suppl 36:78s-86s. 2002
  8. ncbi request reprint Microbiology. TB--a new target, a new drug
    Stewart T Cole
    Génétique Moléculaire Bactérienne and Biochimie Structurale Units, Institut Pasteur, Paris 75724 Cedex 15, France
    Science 307:214-5. 2005
  9. ncbi request reprint Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays
    S V Gordon
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 32:643-55. 1999
  10. ncbi request reprint Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Microbiology 142:3147-61. 1996

Detail Information

Publications109 found, 100 shown here

  1. ncbi request reprint Learning from the genome sequence of Mycobacterium tuberculosis H37Rv
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    FEBS Lett 452:7-10. 1999
    ..41 Mb genome sequence and provided fresh insight into the biochemistry, physiology. genetics and immunology of this much-feared bacterium. Genomic information is centralised in TubercuList (http://www.pasteur.fr/Bio/TubercuList/)...
  2. ncbi request reprint Massive gene decay in the leprosy bacillus
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Nature 409:1007-11. 2001
    ....
  3. ncbi request reprint Why sequence the genome of Mycobacterium tuberculosis?
    S T Cole
    Unité de Génétique Moléculaire, Bactérienne, Institut Pasteur, Paris, France
    Tuber Lung Dis 77:486-90. 1996
    ....
  4. ncbi request reprint Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
    S T Cole
    Sanger Centre, Wellcome Trust Genome Campus, Hinxton, UK
    Nature 393:537-44. 1998
    ....
  5. ncbi request reprint Repetitive sequences in Mycobacterium leprae and their impact on genome plasticity
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, France
    Lepr Rev 72:449-61. 2001
    ..Evidence is presented that loss of synteny, inversion and genome downsizing may have resulted from recombination between dispersed copies of these repetitive elements...
  6. ncbi request reprint Comparative mycobacterial genomics
    S T Cole
    Unité de Génétique Moléculaire Bactérienne Institut Pasteur 28 rue du Docteur Roux 75724 Paris Cedex 15 France
    Curr Opin Microbiol 1:567-71. 1998
    ..leprae genome appears to have undergone shrinkage and large-scale gene inactivation, which may account for the exceptionally slow growth of this organism...
  7. ncbi request reprint Comparative mycobacterial genomics as a tool for drug target and antigen discovery
    S T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Eur Respir J Suppl 36:78s-86s. 2002
    ..Genomics is also furthering tuberculosis vaccine development by pinpointing potentially antigenic proteins as well as providing better diagnostic tools to detect infection...
  8. ncbi request reprint Microbiology. TB--a new target, a new drug
    Stewart T Cole
    Génétique Moléculaire Bactérienne and Biochimie Structurale Units, Institut Pasteur, Paris 75724 Cedex 15, France
    Science 307:214-5. 2005
  9. ncbi request reprint Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays
    S V Gordon
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 32:643-55. 1999
    ..These deletions affect a further seven genes, including a fourth phospholipase, plcD. In summary, the insertions and deletions described here have important implications for our understanding of the evolution of the tubercle complex...
  10. ncbi request reprint Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Microbiology 142:3147-61. 1996
    ..Among the many other noteworthy features were ORFs encoding putative serine/threonine protein kinases and a protein phosphatase, three tRNA genes, one M. leprae-specific repetitive element and a glnQ pseudogene...
  11. ncbi request reprint Regulation of catalase-peroxidase (KatG) expression, isoniazid sensitivity and virulence by furA of Mycobacterium tuberculosis
    A S Pym
    , Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 40:879-89. 2001
    ..These do not include the oxidative stress genes ahpC and sodA, or those for siderophore production...
  12. ncbi request reprint On the catalase-peroxidase gene, katG, of Mycobacterium leprae and the implications for treatment of leprosy with isoniazid
    K Eiglmeier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    FEMS Microbiol Lett 149:273-8. 1997
    ..Isoniazid is thus likely to be of no therapeutic benefit to leprosy patients...
  13. pmc An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae
    W J Philipp
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Proc Natl Acad Sci U S A 93:3132-7. 1996
    ..Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom...
  14. ncbi request reprint Enhanced protection against tuberculosis by vaccination with recombinant Mycobacterium microti vaccine that induces T cell immunity against region of difference 1 antigens
    Priscille Brodin
    Unités de Génétique Moléculaire Bactérienne, INSERM E0352, Institut Pasteur, Paris, France
    J Infect Dis 190:115-22. 2004
    ..microti alone or with BCG. The M. microti OV254::RD1-2F9 vaccine was less virulent and persistent in mice and than was BCG::RD1-2F9 may represent a safer alternative to BCG::RD1-2F9...
  15. ncbi request reprint Molecular genetic analysis of the nagH gene encoding a hyaluronidase of Clostridium perfringens
    B Canard
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Gen Genet 243:215-24. 1994
    ..Two other reading frames, linked to nagH, also appear to encode proteins with sugar-binding motifs...
  16. pmc Homing events in the gyrA gene of some mycobacteria
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Proc Natl Acad Sci U S A 93:3410-5. 1996
    ....
  17. pmc Characterization of the katG gene encoding a catalase-peroxidase required for the isoniazid susceptibility of Mycobacterium tuberculosis
    B Heym
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 175:4255-9. 1993
    ..tuberculosis, M. smegmatis, and Escherichia coli was demonstrated by Western blotting (immunoblotting). Homologous genes were detected in other mycobacteria, even those which are naturally insensitive to isoniazid...
  18. ncbi request reprint A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae
    N Honore
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Int J Lepr Other Mycobact Dis 61:600-4. 1993
    ..The reliability of the method has been tested on a sample of known drug-resistant and susceptible isolates of M. leprae...
  19. ncbi request reprint Comparative genomics of the mycobacteria
    R Brosch
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Int J Med Microbiol 290:143-52. 2000
    ..This minireview summarises the different techniques that have been employed to compare these genomes and gives an overview of the wealth of data that has already been generated by mycobacterial comparative genomics...
  20. ncbi request reprint The decaying genome of Mycobacterium leprae
    K Eiglmeier
    , Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris, France
    Lepr Rev 72:387-98. 2001
    ..This may explain the unusually long generation time and account for our inability to culture the leprosy bacillus...
  21. ncbi request reprint Role of OxyS of Mycobacterium tuberculosis in oxidative stress: overexpression confers increased sensitivity to organic hydroperoxides
    P Domenech
    , Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbes Infect 3:713-21. 2001
    ....
  22. pmc A new evolutionary scenario for the Mycobacterium tuberculosis complex
    R Brosch
    Unite de Genetique Moleculaire Bacterienne, Laboratoire de Génomique des Microorganismes Pathogènes, and Centre National de Référence des Mycobactéries, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 99:3684-9. 2002
    ..bovis lineage separated from the M. tuberculosis lineage. This observation suggests that the common ancestor of the tubercle bacilli resembled M. tuberculosis or M. canettii and could well have been a human pathogen already...
  23. ncbi request reprint The evolution of mycobacterial pathogenicity: clues from comparative genomics
    R Brosch
    , Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Trends Microbiol 9:452-8. 2001
    ..Comparative genomic analyses also suggest that loss of genes is part of the ongoing evolution of the slow-growing mycobacterial pathogens and might also explain how the vaccine strain BCG became attenuated...
  24. ncbi request reprint Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacterium microti
    Alexander S Pym
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 46:709-17. 2002
    ..Knocking-in five other RD loci did not affect the virulence of BCG. This study describes a genetic lesion that contributes to safety and opens new avenues for vaccine development...
  25. ncbi request reprint The carboxy-terminal C2-like domain of the alpha-toxin from Clostridium perfringens mediates calcium-dependent membrane recognition
    I Guillouard
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Microbiol 26:867-76. 1997
    ..Mutations to this domain may account for the natural lack of toxicity of the alpha-toxin homologue, phospholipase C of Clostridium bifermentans...
  26. pmc Streptomycin resistance in mycobacteria
    N Honore
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Antimicrob Agents Chemother 38:238-42. 1994
    ..avium, M. gordonae, and M. szulgai, that are naturally resistant to streptomycin. This suggests that permeability barriers may be responsible for the resistance to the antibiotic...
  27. ncbi request reprint Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid resistance in Mycobacterium tuberculosis
    B Heym
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Microbiol 15:235-45. 1995
    ..The second group comprises a frequently occurring amino acid substitution and a single mutation that are both located in the C-terminal domain but do not noticeably alter either enzyme activity or heat stability...
  28. ncbi request reprint Mycobacterium tuberculosis in the post-genomic age
    P Domenech
    , Institut Pasteur, 28 rue du Docteur Roux, 75724 Cedex 15, Paris, France
    Curr Opin Microbiol 4:28-34. 2001
    ....
  29. ncbi request reprint The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne
    E Cornillot
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Microbiol 15:639-47. 1995
    ..In these cases, the HindIII repeat was not linked to the cpe gene although this was generally preceded by ORF3...
  30. pmc Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50
    S Katayama
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 177:5680-5. 1995
    ..perfringens, Clostridium beijerinckii, and Bacillus subtilis, revealed a similar order and distribution of key sporulation and heat shock genes which might reflect an ancient evolutionary relationship...
  31. ncbi request reprint The Mycobacterium leprae genome: systematic sequence analysis identifies key catabolic enzymes, ATP-dependent transport systems and a novel polA locus associated with genomic variability
    H Fsihi
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Mol Microbiol 16:909-19. 1995
    ..leprae. This is the first evidence for genomic variability in the leprosy bacillus and might ultimately be useful for developing a molecular test capable of distinguishing between strains of M. leprae...
  32. ncbi request reprint Inactivation of the Mycobacterium tuberculosis Nramp orthologue (mntH) does not affect virulence in a mouse model of tuberculosis
    P Domenech
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    FEMS Microbiol Lett 207:81-6. 2002
    ..In neither of these systems was any loss of fitness associated with inactivation of mntH, demonstrating that Nramp orthologues are not important determinants of mycobacterial virulence...
  33. ncbi request reprint Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis
    Alexander S Pym
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Nat Med 9:533-9. 2003
    ..tuberculosis, showing less severe pathology and reduced dissemination of the pathogen, as compared with control animals immunized with BCG alone...
  34. pmc Bacterial artificial chromosome-based comparative genomic analysis identifies Mycobacterium microti as a natural ESAT-6 deletion mutant
    Priscille Brodin
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75724 Paris Cedex 15, France
    Infect Immun 70:5568-78. 2002
    ..microti-specific deleted regions have been identified (MiD1 to MiD3). Deletion of MiD1 has removed parts of the direct repeat region in M. microti and thus contributes to the characteristic spoligotype of M. microti strains...
  35. ncbi request reprint [Use of the mycobacteria genome to develop new vaccines and therapeutic targets]
    C Demangel
    L Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France
    Med Trop (Mars) 64:221-3. 2004
  36. ncbi request reprint The integrated genome map of Mycobacterium leprae
    K Eiglmeier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28, rue du Dr Roux, 75724 Paris, France
    Lepr Rev 72:462-9. 2001
    ..More than 98% of the M. leprae genome is now covered by overlapping large insert genomic clones representing a renewable source of well defined DNA segments and a powerful tool for functional genomics...
  37. ncbi request reprint Complete nucleotide sequence and genetic organization of the bacteriocinogenic plasmid, pIP404, from Clostridium perfringens
    T Garnier
    Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France
    Plasmid 19:134-50. 1988
    ..Only one of the genes from pIP404 was expressed at a significant level in Escherichia coli, suggesting that the atypical codon usage could represent a major obstacle to heterologous gene expression...
  38. ncbi request reprint A method for rapid detection of rifampicin-resistant isolates of Mycobacterium leprae
    N Honore
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris, France
    Lepr Rev 72:441-8. 2001
    ..The standardized procedure could now be used in a prospective study of potential rifampicin resistance among multibacillary patients...
  39. ncbi request reprint Towards the integration of foreign DNA into the chromosome of Mycobacterium leprae
    K Eiglmeier
    Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris
    Res Microbiol 142:617-22. 1991
    ..tuberculosis. In addition, it is shown that the genes encoding aspartate semialdehyde dehydrogenase, asd, and an anonymous protein antigen recognized by sera from leprosy patients, are linked to the M. leprae att-pSAM2 locus...
  40. ncbi request reprint Modulation of the host immune response by a transient intracellular stage of Mycobacterium ulcerans: the contribution of endogenous mycolactone toxin
    Emmanuelle Coutanceau
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Cell Microbiol 7:1187-96. 2005
    ....
  41. pmc Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence
    Priscille Brodin
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 74:88-98. 2006
    ..The present study has allowed us to identify new aspects of the extRD1 region of M. tuberculosis and to explore its role in the pathogenesis of tuberculosis...
  42. ncbi request reprint Immunogenicity of Mycobacterium ulcerans Hsp65 and protective efficacy of a Mycobacterium leprae Hsp65-based DNA vaccine against Buruli ulcer
    Emmanuelle Coutanceau
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbes Infect 8:2075-81. 2006
    ..ulcerans, such as TB10.4, the 19 kDa antigen, PstS3 and Hsp70, may be interesting to consider as subunit vaccines in future prospects...
  43. pmc Inactivation of Rv2525c, a substrate of the twin arginine translocation (Tat) system of Mycobacterium tuberculosis, increases beta-lactam susceptibility and virulence
    Brigitte Saint-Joanis
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex, France
    J Bacteriol 188:6669-79. 2006
    ..The Tat system thus contributes in multiple ways to survival of the tubercle bacillus...
  44. pmc Protection against Mycobacterium ulcerans lesion development by exposure to aquatic insect saliva
    Laurent Marsollier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    PLoS Med 4:e64. 2007
    ..Thus we set out to investigate whether those individuals might display any immune signatures of exposure to M. ulcerans-free insect predator bites, and whether those could correlate with protection...
  45. pmc Genome plasticity of BCG and impact on vaccine efficacy
    Roland Brosch
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 104:5596-601. 2007
    ..Furthermore, the combined findings suggest that early BCG vaccines may even be superior to the later ones that are more widely used...
  46. pmc Control of M. tuberculosis ESAT-6 secretion and specific T cell recognition by PhoP
    Wafa Frigui
    UP de Pathogénomique Mycobactérienne Intégrée, Institut Pasteur, Paris, France
    PLoS Pathog 4:e33. 2008
    ..This finding established a link between the PhoP regulator and ESAT-6 secretion that opens exciting new perspectives for elucidating virulence regulation in M. tuberculosis...
  47. pmc Use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from Clostridium perfringens
    I Guillouard
    Unité de Génétique Moléculaire Bactaŕienne, Institut Pasteur, Paris, France
    Infect Immun 64:2440-4. 1996
    ....
  48. pmc Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12
    S T Cole
    Biochimie des Régulations Cellulaires, Institut Pasteur, Paris, France
    J Bacteriol 170:2448-56. 1988
    ..Analysis of the GlpC primary structure indicated that the protein lacked extended hydrophobic sequences with the potential to form alpha-helices but did contain several long segments capable of forming transmembrane amphipathic helices...
  49. ncbi request reprint Colonization of the salivary glands of Naucoris cimicoides by Mycobacterium ulcerans requires host plasmatocytes and a macrolide toxin, mycolactone
    Laurent Marsollier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Cell Microbiol 7:935-43. 2005
    ..ulcerans isolates are able to invade the salivary glands, a site where they proliferate. Later, the raptorial legs of Naucoris are covered by M. ulcerans-containing material that displays features of biofilms...
  50. pmc Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR
    Nicolas Berthet
    1Genotyping of Pathogens and Public Health Technological Platform, Institut Pasteur, Paris, France
    BMC Mol Biol 9:77. 2008
    ..We adapted the QuantiTect Whole Transcriptome Kit (Qiagen) to our purposes and designated the method as Whole Transcriptome Amplification (WTA)...
  51. pmc Differential effects of prior exposure to environmental mycobacteria on vaccination with Mycobacterium bovis BCG or a recombinant BCG strain expressing RD1 antigens
    Caroline Demangel
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Infect Immun 73:2190-6. 2005
    ..In hosts sensitized with M. avium, potent immunogenicity of ESAT-6 and increased persistence of BCG::RD1 may allow this recombinant vaccine to overcome preexisting antimycobacterial responses...
  52. ncbi request reprint Influence of ESAT-6 secretion system 1 (RD1) of Mycobacterium tuberculosis on the interaction between mycobacteria and the host immune system
    Laleh Majlessi
    Unité de Biologie des Régulations Immunitaires, Institut Pasteur, Institut National de la Santé et de la Recherche Médicale Equipe 352, Paris, France
    J Immunol 174:3570-9. 2005
    ..These modifications can explain the previously described improved protective capacity of BCG::RD1 vaccine candidate against M. tuberculosis challenge...
  53. pmc A point mutation in the two-component regulator PhoP-PhoR accounts for the absence of polyketide-derived acyltrehaloses but not that of phthiocerol dimycocerosates in Mycobacterium tuberculosis H37Ra
    Marie Laure Chesne-Seck
    Laboratoire de Biochimie et Biophysique des Macromolécules URA2185 CNRS, Paris, France
    J Bacteriol 190:1329-34. 2008
    ....
  54. ncbi request reprint Tuberculosis: from genome to vaccine
    Marien I de Jonge
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 Rue du Docteur Roux 75724 Paris, France
    Expert Rev Vaccines 4:541-51. 2005
    ..For designing subunit vaccines, attenuated or improved recombinant whole-cell live vaccines, information from the genomes of the human host and pathogenic mycobacterial species is of great help...
  55. ncbi request reprint Mycobacterial Ser/Thr protein kinases and phosphatases: physiological roles and therapeutic potential
    Annemarie Wehenkel
    Unité de Biochimie Structurale, URA 2185 CNRS, Institut Pasteur, 25 rue du Docteur Roux, F 75724, Paris Cedex 15, France
    Biochim Biophys Acta 1784:193-202. 2008
    ..tuberculosis, their regulation mechanisms and putative substrates, and we explore their therapeutic potential as possible targets for the development of new anti-mycobacterial compounds...
  56. pmc An increase in antimycobacterial Th1-cell responses by prime-boost protocols of immunization does not enhance protection against tuberculosis
    Laleh Majlessi
    Biologie des Régulations Immunitaires, INSERM, E 352, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 74:2128-37. 2006
    ..These results question the broadly admitted correlation between the frequency of IFN-gamma-producing CD4+ T cells and the level of protection against tuberculosis...
  57. pmc Selective suppression of dendritic cell functions by Mycobacterium ulcerans toxin mycolactone
    Emmanuelle Coutanceau
    Unite de Genetique Moleculaire Bacterienne, Genomes and Genetics Department, Institut Pasteur, 75724 Paris, Cedex 15, France
    J Exp Med 204:1395-403. 2007
    ..Moreover, they highlight a potential interest in mycolactone as a novel immunosuppressive agent...
  58. ncbi request reprint Early trafficking events of Mycobacterium ulcerans within Naucoris cimicoides
    Laurent Marsollier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Cell Microbiol 9:347-55. 2007
    ..ulcerans into the HC, in contrast to the next step, colonization of the salivary glands; second, M. ulcerans clusters bind a member of the serpin protein family present in the salivary gland homogenate...
  59. pmc Impact of Mycobacterium ulcerans biofilm on transmissibility to ecological niches and Buruli ulcer pathogenesis
    Laurent Marsollier
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    PLoS Pathog 3:e62. 2007
    ..The results of this study support a model whereby biofilm changes confer selective advantages to M. ulcerans in colonizing various ecological niches successfully, with repercussions for Buruli ulcer pathogenesis...
  60. pmc Reductive evolution and niche adaptation inferred from the genome of Mycobacterium ulcerans, the causative agent of Buruli ulcer
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75725 Paris Cedex 15, France
    Genome Res 17:192-200. 2007
    ..ulcerans and M. marinum. This work offers significant insight into the biology and evolution of mycobacterial pathogens and is an important component of international efforts to counter Buruli ulcer...
  61. pmc Effect of katG mutations on the virulence of Mycobacterium tuberculosis and the implication for transmission in humans
    Alexander S Pym
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Infect Immun 70:4955-60. 2002
    ..The implications of this finding for the transmission and reactivation of multidrug-resistant strains of M. tuberculosis are severe...
  62. pmc Cell envelope protein PPE68 contributes to Mycobacterium tuberculosis RD1 immunogenicity independently of a 10-kilodalton culture filtrate protein and ESAT-6
    Caroline Demangel
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 75724 Paris Cedex 15, France
    Infect Immun 72:2170-6. 2004
    ..Taken together, these results show that PPE68 is an immunogenic product of the RD1 region, which does not interfere with the secretion and immunogenicity of CFP-10 and ESAT-6...
  63. ncbi request reprint Comparative and functional genomics of the Mycobacterium tuberculosis complex
    Stewart T Cole
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 148:2919-28. 2002
  64. pmc Aquatic snails, passive hosts of Mycobacterium ulcerans
    Laurent Marsollier
    Unité de Génétique bactérienne, Institut Pasteur, 75724 Paris, France
    Appl Environ Microbiol 70:6296-8. 2004
    ..Water bugs, such as Naucoris cimicoides, a potential vector of M. ulcerans, were shown to be infected specifically by this bacterium after feeding on snails experimentally exposed to M. ulcerans...
  65. ncbi request reprint Proteomic identification of M. tuberculosis protein kinase substrates: PknB recruits GarA, a FHA domain-containing protein, through activation loop-mediated interactions
    A Villarino
    Unité de Biochimie Structurale URA 2185 CNRS, Institut Pasteur, Paris, France
    J Mol Biol 350:953-63. 2005
    ..The ensuing model of PknB-GarA interactions suggests a substrate recruitment mechanism that might apply to other mycobacterial kinases bearing multiple phosphorylation sites in their activation loops...
  66. ncbi request reprint Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv
    Jean Christophe Camus
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex, France
    Microbiology 148:2967-73. 2002
    ..Presently, it is possible to assign a function to 2058 proteins (52% of the 3995 proteins predicted) and only 376 putative proteins share no homology with known proteins and thus could be unique to M. tuberculosis...
  67. ncbi request reprint ESAT-6 proteins: protective antigens and virulence factors?
    Priscille Brodin
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex, France
    Trends Microbiol 12:500-8. 2004
    ..Here, we discuss these findings and their implications for the development of new therapeutic and prophylactic interventions against tuberculosis...
  68. doi request reprint Diversity-oriented synthesis of furo[3,2-f]chromanes with antimycobacterial activity
    Luke Alvey
    Institut Pasteur, URA 2128 CNRS Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Eur J Med Chem 44:2497-505. 2009
    ..However, the many analogues which inhibited the growth of M. tuberculosis in the 0.6-5 microg/mL range turned out to be also cytotoxic on VERO cells growth at the same concentration range...
  69. ncbi request reprint Molecular characterization of the resolvase gene, res, carried by a multicopy plasmid from Clostridium perfringens: common evolutionary origin for prokaryotic site-specific recombinases
    T Garnier
    Unité des Applications du Génie Génétique, Institut Pasteur, Paris, France
    Mol Microbiol 1:371-6. 1987
    ..Phylogenetic analysis of the primary structures of ten site-specific recombinases suggested a common descent and showed the RES protein to be closest to the resolvase encoded by Tn917 from Streptococcus faecalis...
  70. pmc Antigen discovery: a postgenomic approach to leprosy diagnosis
    Romulo Araoz
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Infect Immun 74:175-82. 2006
    ..Among them, two class II proteins, ML0308 and ML2498, showed marked humoral and cellular immunogenicity, therefore providing promising candidates for the diagnosis of both tuberculoid and lepromatous forms of leprosy...
  71. doi request reprint Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis
    Petra Tafelmeyer
    Plate forme de Protéomique, Institut Pasteur, Paris, France
    Proteomics 8:3124-38. 2008
    ....
  72. pmc The complete genome sequence of Mycobacterium bovis
    Thierry Garnier
    Unité de Génétique Moléculaire Bactérienne and PT4 Annotation, Genopole, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 100:7877-82. 2003
    ..bovis, implying that differential gene expression may be the key to the host tropisms of human and bovine bacilli. The genome sequence therefore offers major insight on the evolution, host preference, and pathobiology of M. bovis...
  73. ncbi request reprint PknB kinase activity is regulated by phosphorylation in two Thr residues and dephosphorylation by PstP, the cognate phospho-Ser/Thr phosphatase, in Mycobacterium tuberculosis
    Brigitte Boitel
    Unité de Biochimie Structurale, URA 2185 CNRS, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France
    Mol Microbiol 49:1493-508. 2003
    ....
  74. ncbi request reprint Towards an immunodiagnostic test for leprosy
    Romulo Araoz
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbes Infect 8:2270-6. 2006
    ..This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy...
  75. ncbi request reprint Synthesis and antimycobacterial evaluation of benzofurobenzopyran analogues
    Soizic Prado
    Laboratoire de Chimie Organique, URA 2128 CNRS Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
    Bioorg Med Chem 15:2177-86. 2007
    ..We wish to report here the preparation of some structural analogues along with their biological effect on the growth of Mycobacterium smegmatis, M. tuberculosis, as well as on VERO cells for the most active compound...
  76. ncbi request reprint Macro-array and bioinformatic analyses reveal mycobacterial 'core' genes, variation in the ESAT-6 gene family and new phylogenetic markers for the Mycobacterium tuberculosis complex
    Magali Marmiesse
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Microbiology 150:483-96. 2004
    ..tuberculosis complex recently presented...
  77. pmc The Ser/Thr protein kinase PknB is essential for sustaining mycobacterial growth
    Pablo Fernandez
    Unité de Biochimie Structurale, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
    J Bacteriol 188:7778-84. 2006
    ..These findings demonstrate that the Ser/Thr protein kinase PknB is essential for sustaining mycobacterial growth and support the development of protein kinase inhibitors as new potential antituberculosis drugs...
  78. pmc ESAT-6 from Mycobacterium tuberculosis dissociates from its putative chaperone CFP-10 under acidic conditions and exhibits membrane-lysing activity
    Marien I de Jonge
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 189:6028-34. 2007
    ..tuberculosis is the interaction with biomembranes that occurs after dissociation from its putative chaperone CFP-10 under acidic conditions typically encountered in the phagosome...
  79. ncbi request reprint Are the PE-PGRS proteins of Mycobacterium tuberculosis variable surface antigens?
    Sayera Banu
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
    Mol Microbiol 44:9-19. 2002
    ..Subcellular fractionation studies and immunoelectron microscopy localized many PE-PGRS proteins in the cell wall and cell membrane of M. tuberculosis. The data suggest that some PE-PGRS proteins are variable surface antigens...
  80. ncbi request reprint Structure and mechanism of the alkyl hydroperoxidase AhpC, a key element of the Mycobacterium tuberculosis defense system against oxidative stress
    Beatriz G Guimarães
    Unité de Biochimie Structurale, CNRS URA 2185, 25 rue du Docteur Roux and Unité de Génétique Moléculaire Bactérienne, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris
    J Biol Chem 280:25735-42. 2005
    ....
  81. ncbi request reprint Functional analysis and annotation of the virulence plasmid pMUM001 from Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
    Microbiology 151:683-92. 2005
    ..The results presented here provide important insights into this unusual virulence plasmid from an emerging but neglected human pathogen...
  82. ncbi request reprint Molecular genetic analysis of FNR-dependent promoters
    K Eiglmeier
    Laboratoire de Génétique Moléculaire Bactérienne, Institut Pasteur, Paris, France
    Mol Microbiol 3:869-78. 1989
    ..Studies with two mutant frd promoters from Escherichia coli, displaying altered regulation and FNR response, provided additional evidence for recognition of this sequence by FNR...
  83. pmc Use of consensus sequences for the design of high density resequencing microarrays: the influenza virus paradigm
    India Leclercq
    Institut Pasteur, Laboratory for Urgent Response to Biological Threats CIBU, Paris, France
    BMC Genomics 11:586. 2010
    ..The part dedicated to influenza viruses was based on consensus sequences specific for one gene generated from global alignments of a large number of influenza virus sequences available in databanks...
  84. ncbi request reprint On the origin of leprosy
    Marc Monot
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    Science 308:1040-2. 2005
    ..The disease seems to have originated in Eastern Africa or the Near East and spread with successive human migrations. Europeans or North Africans introduced leprosy into West Africa and the Americas within the past 500 years...
  85. pmc Are variable-number tandem repeats appropriate for genotyping Mycobacterium leprae?
    Marc Monot
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Clin Microbiol 46:2291-7. 2008
    ..leprae but also between biopsy samples from the same patient, these VNTR may be too dynamic for use as epidemiological markers for leprosy...
  86. pmc The crystal structure of Rv0813c from Mycobacterium tuberculosis reveals a new family of fatty acid-binding protein-like proteins in bacteria
    William Shepard
    Unité de Biochimie Structurale, CNRS URA 2185, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris, France
    J Bacteriol 189:1899-904. 2007
    ....
  87. pmc Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Institut Pasteur, Paris, France
    J Bacteriol 187:1668-76. 2005
    ..Our results suggest that pMUM is a defining characteristic of M. ulcerans but that in the absence of purifying selection, deletion of plasmid sequences and a corresponding loss of mycolactone production readily arise...
  88. pmc Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans
    Timothy P Stinear
    Unite de Genetique Moleculaire Bacterienne, Genopole, Institut Pasteur, 28 rue du Docteur Roux, 75725 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 101:1345-9. 2004
    ..Such detailed knowledge of mycolactone will further the investigation of its mode of action and the development of urgently needed therapeutic strategies to combat Buruli ulcer...
  89. pmc The crystal structure of M. leprae ML2640c defines a large family of putative S-adenosylmethionine-dependent methyltransferases in mycobacteria
    Martin Grana
    Unité de Biochimie Structurale CNRS URA 2185, Institut Pasteur, 75724 Paris, France
    Protein Sci 16:1896-904. 2007
    ..The ML2640c crystal structures offer the first structural glimpses at this important family of mycobacterial proteins and lend strong support to their functional assignment as AdoMet-dependent methyltransferases...
  90. pmc Application of broad-spectrum resequencing microarray for genotyping rhabdoviruses
    Laurent Dacheux
    Institut Pasteur, Lyssavirus Dynamics and Host Adaptation Unit, Paris, France
    J Virol 84:9557-74. 2010
    ....
  91. doi request reprint Comparative genomic and phylogeographic analysis of Mycobacterium leprae
    Marc Monot
    Institut Pasteur, Paris, France
    Nat Genet 41:1282-9. 2009
    ..The 16 SNP subtypes showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy...
  92. doi request reprint Buruli ulcer: reductive evolution enhances pathogenicity of Mycobacterium ulcerans
    Caroline Demangel
    Institut Pasteur, UP Pathogénomique Mycobactérienne Intégrée, Paris, France
    Nat Rev Microbiol 7:50-60. 2009
    ..Here, the ecology, microbiology, evolutionary genomics and immunopathology of Buruli ulcer are reviewed...
  93. doi request reprint A new synthetic access to furo[3,2-f]chromene analogues of an antimycobacterial
    Luke Alvey
    Laboratoire de Chimie Organique, URA 2128 CNRS Institut Pasteur, 25 28 Rue du Docteur Roux, 75724 Paris Cedex 15, France
    Bioorg Med Chem 16:8264-72. 2008
    ..Their biological evaluation on the growth of Mycobacterium smegmatis as well as Mycobacterium tuberculosis pointed out that some analogues were four times more active than the initial hit...
  94. pmc New approaches to filling the gap in tuberculosis drug discovery
    Martina Casenghi
    Medecins Sans Frontieres, Campaign for Access to Essential Medicines, Geneva
    PLoS Med 4:e293. 2007
  95. pmc Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis
    Timothy P Stinear
    Department of Microbiology, Monash University, Clayton 3800, Australia
    Genome Res 18:729-41. 2008
    ..tuberculosis. The work described herein provides a foundation for using M. marinum to better understand the determinants of pathogenesis of tuberculosis...
  96. doi request reprint A novel drug discovery concept for tuberculosis: inhibition of bacterial and host cell signalling
    Rita Székely
    Vichem Chemie Research Ltd, Herman Ottó 15, H 1022 Budapest, Hungary
    Immunol Lett 116:225-31. 2008
    ..In the course of our future work, we will increase the potency of the next generation of PknB inhibitors in order to improve their antibacterial activity...
  97. ncbi request reprint Royal Society of Tropical Medicine and Hygiene Meeting at Manson House, London, 18th January 2001. Pathogen genomes and human health. Mycobacterial genomics
    Stephen V Gordon
    Veterinary Laboratories Agency, Woodham Lane, New Haw, Addlestone, Surrey KT15 3NB, UK
    Trans R Soc Trop Med Hyg 96:1-6. 2002
    ..This article will focus on what the genome sequences of M. tuberculosis and M. leprae have taught us about these pathogens, and how comparative genomics has exposed some of the fundamental differences between the species...
  98. pmc Mycobacterium tuberculosis and the host response
    Stefan H E Kaufmann
    Max Plank Institute for Infection Biology, Berlin D10117, Germany
    J Exp Med 201:1693-7. 2005
    ..tuberculosis and the infected host, the nature of protective immune responses, adaptation of the bacillus to host-imposed stresses, animal models, and new techniques...
  99. pmc Comparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10
    John S Spencer
    Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523, USA
    Infect Immun 72:3161-70. 2004
    ..leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein...
  100. ncbi request reprint Functional analysis of early secreted antigenic target-6, the dominant T-cell antigen of Mycobacterium tuberculosis, reveals key residues involved in secretion, complex formation, virulence, and immunogenicity
    Priscille Brodin
    Unite de Genetique Moleculaire Bacterienne, Unité de Biologie des Régulations Immunitaires, INSERM E352
    J Biol Chem 280:33953-9. 2005
    ..CFP-10 complex to be constructed that is critical for understanding the structure-function relationship in tuberculosis pathogenesis...