Affiliation: Institut de Biologie Structurale
Brutscher B, Simorre J. Transverse relaxation optimized HCN experiment for nucleic acids: combining the advantages of TROSY and MQ spin evolution. J Biomol NMR. 2001;21:367-72 pubmed
..The advantages of the MQ-TROSY-HCN experiment compared to the best previous implementations of HCN are demonstrated for a 33 nucleotide RNA aptamer...
Vallet A, Favier A, Brutscher B. Aromatic SOFAST-HMBC for proteins at natural 13C abundance. J Magn Reson. 2019;300:95-102 pubmed publisher
..Despite the low amount of NMR-active 13C nuclei in these samples, 1H-13C multiple-bond correlation spectra of good quality were obtained in reasonable experimental times of typically less than 24 h. ..
Christou N, Brutscher B. BEST and SOFAST experiments for resonance assignment of histidine and tyrosine side chains in 13C/15N labeled proteins. J Biomol NMR. 2018;72:115-124 pubmed publisher
HoÅ¡ek T, Gil Caballero S, Pierattelli R, Brutscher B, Felli I. Longitudinal relaxation properties of (1)H(N) and (1)H(Î±) determined by direct-detected (13)C NMR experiments to study intrinsically disordered proteins (IDPs). J Magn Reson. 2015;254:19-26 pubmed publisher
..The proposed experiments were tested on human Î±-synuclein and ubiquitin, two representative examples of unfolded and folded proteins. ..
Brutscher B, Felli I, Gil Caballero S, HoÅ¡ek T, KÃ¼mmerle R, Piai A, et al
. NMR Methods for the Study of Instrinsically Disordered Proteins Structure, Dynamics, and Interactions: General Overview and Practical Guidelines. Adv Exp Med Biol. 2015;870:49-122 pubmed publisher
..3.7), sequential NMR assignment: 3D experiments (Sect. 3.8), high-dimensional NMR experiments (nD, with n>3) (Sect. 3.9) and conclusions and perspectives (Sect. 3.10). ..
SÃ³lyom Z, Ma P, Schwarten M, Bosco M, Polidori A, Durand G, et al
. The Disordered Region of the HCV Protein NS5A: Conformational Dynamics, SH3 Binding, and Phosphorylation. Biophys J. 2015;109:1483-96 pubmed publisher
Franco R, Favier A, Schanda P, Brutscher B. Optimized fast mixing device for real-time NMR applications. J Magn Reson. 2017;281:125-129 pubmed publisher
Baronti L, HoÅ¡ek T, Gil Caballero S, Raveh Amit H, CalÃ§ada E, Ayala I, et al
. Fragment-Based NMR Study of the Conformational Dynamics in the bHLH Transcription Factor Ascl1. Biophys J. 2017;112:1366-1373 pubmed publisher
..Our results contribute to a better understanding of the mechanisms of action that govern the regulation of proneural transcription factors. ..