Philippe Bousso

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. doi request reprint T-cell activation by dendritic cells in the lymph node: lessons from the movies
    Philippe Bousso
    G5 Dynamiques des Réponses Immunea, Equipe Avenir, Inserm U668, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris 15, France
    Nat Rev Immunol 8:675-84. 2008
  2. ncbi request reprint Real-time imaging of T-cell development
    Philippe Bousso
    Institut National de la Sante et de la Recherche Medicale U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Curr Opin Immunol 16:400-5. 2004
  3. ncbi request reprint Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes
    Philippe Bousso
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Immunol 4:579-85. 2003
  4. ncbi request reprint Facing two T cell epitopes: a degree of randomness in the primary response is lost upon secondary immunization
    P Bousso
    Unité de Biologie Moléculaire du Gène, Institut National de la Sante et de la Recherche Medicale, Unité 277, Institut Pasteur, Paris, France
    J Immunol 165:760-7. 2000
  5. pmc The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones
    P Bousso
    Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale U277 and Institut Pasteur, 75724 Paris, France
    J Exp Med 189:1591-600. 1999
  6. ncbi request reprint A clonal view of alphabeta T cell responses
    P Bousso
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, 25 rue du Dr Roux, Paris, Cedex 15, 75015 75724, France
    Semin Immunol 11:423-31. 1999
  7. ncbi request reprint Cutting edge: cognate CD4 help promotes recruitment of antigen-specific CD8 T cells around dendritic cells
    Hélène Beuneu
    G5 Dynamiques des Reponses Immunes, Equipe Avenir Inserm U668, Institut Pasteur, Paris, France
    J Immunol 177:1406-10. 2006
  8. doi request reprint Intravital imaging reveals distinct dynamics for natural killer and CD8(+) T cells during tumor regression
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 33:632-44. 2010
  9. pmc Competition for antigen determines the stability of T cell-dendritic cell interactions during clonal expansion
    Zacarias Garcia
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, F 75015 Paris, France
    Proc Natl Acad Sci U S A 104:4553-8. 2007
  10. doi request reprint Visualizing the functional diversification of CD8+ T cell responses in lymph nodes
    Hélène Beuneu
    Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Departement d Immunologie, Paris, France
    Immunity 33:412-23. 2010

Collaborators

Detail Information

Publications47

  1. doi request reprint T-cell activation by dendritic cells in the lymph node: lessons from the movies
    Philippe Bousso
    G5 Dynamiques des Réponses Immunea, Equipe Avenir, Inserm U668, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris 15, France
    Nat Rev Immunol 8:675-84. 2008
    ..This Review focuses on how this emerging field is changing our perception of T-cell activation by DCs...
  2. ncbi request reprint Real-time imaging of T-cell development
    Philippe Bousso
    Institut National de la Sante et de la Recherche Medicale U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Curr Opin Immunol 16:400-5. 2004
    ..Resolving thymocyte behavior at the single-cell level should help to clarify the mode of trafficking in the thymus and identify the spatio-temporal aspects of thymic selection events...
  3. ncbi request reprint Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes
    Philippe Bousso
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Immunol 4:579-85. 2003
    ..Taken together, our results identify the cellular behaviors that promote an efficient CD8+ T cell response in the lymph node...
  4. ncbi request reprint Facing two T cell epitopes: a degree of randomness in the primary response is lost upon secondary immunization
    P Bousso
    Unité de Biologie Moléculaire du Gène, Institut National de la Sante et de la Recherche Medicale, Unité 277, Institut Pasteur, Paris, France
    J Immunol 165:760-7. 2000
    ..We conclude that, at least for this set of epitopes, the CTL hierarchy is not controlled by the same parameters in a primary vs a secondary response...
  5. pmc The composition of a primary T cell response is largely determined by the timing of recruitment of individual T cell clones
    P Bousso
    Unité de Biologie Moléculaire du Gène, Institut National de la Santé et de la Recherche Médicale U277 and Institut Pasteur, 75724 Paris, France
    J Exp Med 189:1591-600. 1999
    ..Therefore, the contribution of a T cell clone to the immune response is mostly determined by the time of its entry into the immune repertoire, i.e., the time of first cell division after antigen encounter...
  6. ncbi request reprint A clonal view of alphabeta T cell responses
    P Bousso
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, 25 rue du Dr Roux, Paris, Cedex 15, 75015 75724, France
    Semin Immunol 11:423-31. 1999
    ..Emphasis is placed on the role of the naive alphabeta T cell repertoire and on the dynamics of individual Ag-specific T cell clones during the successive phases of an immune response...
  7. ncbi request reprint Cutting edge: cognate CD4 help promotes recruitment of antigen-specific CD8 T cells around dendritic cells
    Hélène Beuneu
    G5 Dynamiques des Reponses Immunes, Equipe Avenir Inserm U668, Institut Pasteur, Paris, France
    J Immunol 177:1406-10. 2006
    ..Our results support a model in which CD4 help operates rapidly, in part by favoring CD8 T cells recruitment around those DCs that are the most competent for priming...
  8. doi request reprint Intravital imaging reveals distinct dynamics for natural killer and CD8(+) T cells during tumor regression
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 33:632-44. 2010
    ..Altogether, our results offer mechanistic insight into how NK cells and CTLs can exert cytotoxic activity with remarkably different contact dynamics...
  9. pmc Competition for antigen determines the stability of T cell-dendritic cell interactions during clonal expansion
    Zacarias Garcia
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, F 75015 Paris, France
    Proc Natl Acad Sci U S A 104:4553-8. 2007
    ....
  10. doi request reprint Visualizing the functional diversification of CD8+ T cell responses in lymph nodes
    Hélène Beuneu
    Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Departement d Immunologie, Paris, France
    Immunity 33:412-23. 2010
    ..Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies...
  11. doi request reprint Decoding the dynamics of T cell-dendritic cell interactions in vivo
    Susanna Celli
    G5 Dynamiques des Reponses Immunes, Departement d Immunologie, Institut Pasteur, Paris, France
    Immunol Rev 221:182-7. 2008
    ....
  12. doi request reprint Cutting Edge: A dual role for type I IFNs during polyinosinic-polycytidylic acid-induced NK cell activation
    Hélène Beuneu
    Institut Pasteur, Dynamics of Immune Responses Unit, F 75015 Paris, France
    J Immunol 187:2084-8. 2011
    ..Our results suggest that type I IFNs produced following viral infection simultaneously target accessory cells for IL-15 transpresentation and NK cells themselves and that these two pathways cooperate for NK cell priming...
  13. ncbi request reprint Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation
    Susanna Celli
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, F 75015, France
    Immunity 27:625-34. 2007
    ..These results establish the importance of prolonged T cell-DC interactions for efficient CD4(+) T cell activation in vivo...
  14. doi request reprint Dynamic behavior of NK cells during activation in lymph nodes
    Hélène Beuneu
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, France
    Blood 114:3227-34. 2009
    ..That NK cells become activated while remaining motile may constitute an efficient strategy for sampling local concentrations of cytokines around DCs in secondary lymphoid tissues...
  15. doi request reprint Subcapsular sinus macrophages promote NK cell accumulation and activation in response to lymph-borne viral particles
    Zacarias Garcia
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Blood 120:4744-50. 2012
    ..Our results identify SCS macrophages as primary mediators of NK cell activation in response to lymph-borne viral particles suggesting that they act as early sensors of local infection or delivery of viral-based vaccines...
  16. pmc Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice
    Beatrice Breart
    G5 Dynamiques des Reponses Immunes, Institut Pasteur, Paris, France
    J Clin Invest 118:1390-7. 2008
    ..The ability to visualize when, where, and how tumor cells are killed in vivo offers new perspectives for understanding how immune effectors survey cancer cells and how local tumor microenvironments may subvert immune responses...
  17. ncbi request reprint Dynamic behavior of T cells and thymocytes in lymphoid organs as revealed by two-photon microscopy
    Philippe Bousso
    Unité U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Immunity 21:349-55. 2004
    ..In this review we summarize what we have learned so far and point out areas for future investigation using this approach...
  18. doi request reprint Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes
    Susanna Celli
    Departement d Immunologie, G5 Dynamiques des Reponses Immunes, Equipe Avenir, Institut Pasteur, Paris Cedex, France
    Methods Mol Biol 415:119-26. 2008
    ..Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings...
  19. ncbi request reprint Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy
    Philippe Bousso
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Science 296:1876-80. 2002
    ..Our results identify the diversity and dynamics of thymocyte interactions during positive selection...
  20. ncbi request reprint Intravital two-photon imaging of T-cell priming and tolerance in the lymph node
    Susanna Celli
    Department d Immunologie, Institut Pasteur, Paris, France
    Methods Mol Biol 380:355-63. 2007
    ..This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts...
  21. pmc CD4 T cells integrate signals delivered during successive DC encounters in vivo
    Susanna Celli
    G5 Dynamiques des Reponses Immunes, INSERM 668, Departement d Immunologie, Institut Pasteur, Paris, France
    J Exp Med 202:1271-8. 2005
    ..Thus, repeated encounters with Ag-bearing DCs can occur in vivo and modulate CD4 T cell differentiation programs...
  22. doi request reprint Cutting edge: tumor-targeting antibodies enhance NKG2D-mediated NK cell cytotoxicity by stabilizing NK cell-tumor cell interactions
    Jacques Deguine
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France
    J Immunol 189:5493-7. 2012
    ..Thus, complementary signals mediated by recognition of stress ligands and tumor-specific Abs may contribute to the efficacy of NK cells during mAb therapy...
  23. doi request reprint Dynamic in situ cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo
    Hélène D Moreau
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 37:351-63. 2012
    ..As illustrated here, the DISC approach should provide unique opportunities to link immune cell behavior to phenotype and function in vivo...
  24. doi request reprint CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation
    Andreas J Müller
    Dynamics of Immune Responses, Institut Pasteur, Paris, France
    Immunity 37:147-57. 2012
    ..Our results thus demonstrate that CD4(+) T cells can exert their protective activity by engaging a minority of infected cells...
  25. pmc Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes
    Georges A Azar
    G5 Dynamiques des Reponses Immunes, Institut Pasteur, 75015 Paris, France
    Proc Natl Acad Sci U S A 107:3675-80. 2010
    ..Furthermore, we provide a dynamic look at immunological kinapses and synapses in lymph nodes and suggest the existence of distinct patterns of LAT redistribution during antigen recognition...
  26. doi request reprint A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection
    Orchidée Filipe-Santos
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Inserm U668, Equipe Avenir, Paris F 75724, France
    Cell Host Microbe 6:23-33. 2009
    ..Thus, disparities in the dynamics of T cell contacts with infected cells and local variation in T cell access to infected cells are important elements of the host-pathogen interplay...
  27. doi request reprint How many dendritic cells are required to initiate a T-cell response?
    Susanna Celli
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Blood 120:3945-8. 2012
    ..In mice and probably humans, we estimate that a minimum of 85 DCs are required to initiate a T-cell response when starting from precursor frequency of 10(-6). Our results have implications for the rational design of DC-based vaccines...
  28. doi request reprint Dissecting T cell contraction in vivo using a genetically encoded reporter of apoptosis
    Kym R Garrod
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France Inserm U668, 75015 Paris, France
    Cell Rep 2:1438-47. 2012
    ..Our results offer insight into a poorly explored phase of immunity and provide a versatile methodology to study apoptosis during the development or function of a variety of immune cells in vivo...
  29. ncbi request reprint Cellular orchestration of T cell priming in lymph nodes
    Beatrice Breart
    G5 Dynamiques des Reponses Immunes, Equipe Avenir, Inserm U668, Institut Pasteur, Paris, France
    Curr Opin Immunol 18:483-90. 2006
    ..Recent studies have begun to depict the cellular orchestration of T cell priming and to analyze the way it could influence the outcome of T cell responses...
  30. doi request reprint Quantifying subcellular distribution of fluorescent fusion proteins in cells migrating within tissues
    Heather J Melichar
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Immunol Cell Biol 89:549-57. 2011
    ..These approaches could be readily adapted to other fluorescent fusion proteins, tissues and biological questions...
  31. ncbi request reprint Chronic infections capture little attention of the masses
    Philippe Bousso
    Dynamics of Immune Responses Unit, Department of Immunology, Institut Pasteur, Paris, France
    Immunity 34:699-701. 2011
    ....
  32. ncbi request reprint The Qa-1b molecule binds to a large subpopulation of murine NK cells
    M Salcedo
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris, France
    Eur J Immunol 28:4356-61. 1998
    ..Our data suggest the existence of a Qa-1-recognizing structure on a large subpopulation of murine NK cells that may be similar to the human CD94/NKG2 heterodimeric complex...
  33. doi request reprint Dynamics of NK cell interactions in vivo
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Immunol Rev 251:154-9. 2013
    ..We review here our recent understanding of NK cell interactions in vivo at steady state, during inflammation, and during tumor elimination, emphasizing the similarities and differences with T cells...
  34. doi request reprint Functional immunoimaging: the revolution continues
    Philippe Bousso
    Institut Pasteur, Dynamics of Immune Responses Unit, F 75015 Paris, France
    Nat Rev Immunol 12:858-64. 2012
    ..These exciting developments further broaden the applications of immunoimaging and provide unprecedented opportunities to probe and decode immune cell communication in situ...
  35. doi request reprint Restricted microbiota and absence of cognate TCR antigen leads to an unbalanced generation of Th17 cells
    Matthias Lochner
    Lymphoid Tissue Development Unit, Department of Immunology, Institut Pasteur, 75724 Paris, France
    J Immunol 186:1531-7. 2011
    ..These results demonstrate that a complex microbiota and cognate Ag are required to generate a properly regulated set of RORγt(+) T cells and Th17 cells...
  36. ncbi request reprint Visualizing thymocyte motility using 2-photon microscopy
    Ellen A Robey
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Immunol Rev 195:51-7. 2003
    ..In this review, we discuss the implications of thymocyte motility for T-cell repertoire selection and for the mechanisms that determine the spatial organization of thymocyte subsets within the thymus...
  37. ncbi request reprint Impact of negative selection on the T cell repertoire reactive to a self-peptide: a large fraction of T cell clones escapes clonal deletion
    C Bouneaud
    Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris, France
    Immunity 13:829-40. 2000
    ..These results suggest that numerous low-affinity self-specific T cells persist in the periphery and show that a deleting ligand can specifically narrow the structural diversity of the TCR repertoire...
  38. doi request reprint Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division
    Fabrice Lemaitre
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France
    J Immunol 191:1578-85. 2013
    ..Our results support the idea that T cell diversification is a continuous process, initiated before clonal expansion and amplified during the first and subsequent cell divisions. ..
  39. pmc Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
    Laure Guenin-Macé
    Institut Pasteur, Unité d Immunobiologie de l Infection, Paris, France
    J Clin Invest 123:1501-12. 2013
    ..Our findings should allow for the rationale design of competitive inhibitors of mycolactone binding to N-WASP, with anti-Buruli ulcer therapeutic potential...
  40. doi request reprint Preexisting BCG-specific T cells improve intravesical immunotherapy for bladder cancer
    Claire Biot
    Unité d Immunobiologie des Cellules Dendritiques, Department of Immunology, Institut Pasteur, F 75724 Paris, France
    Sci Transl Med 4:137ra72. 2012
    ....
  41. doi request reprint Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy
    Susanna Celli
    Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Departement d Immunologie, Paris, France
    Nat Med 17:744-9. 2011
    ....
  42. pmc A role for the immediate early gene product c-fos in imprinting T cells with short-term memory for signal summation
    CAROLYN E CLARK
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    PLoS ONE 6:e18916. 2011
    ....
  43. pmc Shigella impairs T lymphocyte dynamics in vivo
    Wilmara Salgado-Pabon
    Molecular Microbial Pathogenesis Unit, Department Cell Biology and Infection, Institut National de Santé et de Recherche Médicale U786, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 110:4458-63. 2013
    ..These findings indicate that S. flexneri targets T lymphocytes in vivo and highlight the role of type III effector secretion in modulating host adaptive immune responses...
  44. ncbi request reprint Antigen persistence is required for dendritic cell licensing and CD8+ T cell cross-priming
    Hélène Jusforgues-Saklani
    Immunology Department, Institut Pasteur, Paris France
    J Immunol 181:3067-76. 2008
    ..These findings imply that long-lived Ag is critical for efficient vaccination protocols in which the CD8(+) T cell response is helper-dependent...
  45. ncbi request reprint Lymph node choreography: Treg cells join the dance
    Philippe Bousso
    Nat Immunol 7:11-3. 2006
  46. ncbi request reprint TNF-mediated toxicity after massive induction of specific CD8+ T cells following immunization of mice with a tumor-specific peptide
    Janine Bilsborough
    Ludwig Institute for Cancer Research and Cellular Genetics Unit, Universite de Louvain, Brussels, Belgium
    J Immunol 169:3053-60. 2002
    ..Our results suggest that immunotherapy trials involving new peptides should be pursued with caution and should include a careful monitoring of the T cell response...
  47. ncbi request reprint Imaging the immunological synapse
    Sylvia Cohen-Kaminsky
    Eur J Immunol 37:299-300. 2007