Philippe Bousso

Summary

Affiliation: Institut Pasteur
Country: France

Publications

  1. doi Functional immunoimaging: the revolution continues
    Philippe Bousso
    Institut Pasteur, Dynamics of Immune Responses Unit, F 75015 Paris, France
    Nat Rev Immunol 12:858-64. 2012
  2. ncbi Chronic infections capture little attention of the masses
    Philippe Bousso
    Dynamics of Immune Responses Unit, Department of Immunology, Institut Pasteur, Paris, France
    Immunity 34:699-701. 2011
  3. doi Intravital imaging reveals distinct dynamics for natural killer and CD8(+) T cells during tumor regression
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 33:632-44. 2010
  4. doi Decoding the dynamics of T cell-dendritic cell interactions in vivo
    Susanna Celli
    G5 Dynamiques des Reponses Immunes, Departement d Immunologie, Institut Pasteur, Paris, France
    Immunol Rev 221:182-7. 2008
  5. pmc Competition for antigen determines the stability of T cell-dendritic cell interactions during clonal expansion
    Zacarias Garcia
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, F 75015 Paris, France
    Proc Natl Acad Sci U S A 104:4553-8. 2007
  6. doi Dynamic behavior of NK cells during activation in lymph nodes
    Hélène Beuneu
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, France
    Blood 114:3227-34. 2009
  7. ncbi Cutting edge: cognate CD4 help promotes recruitment of antigen-specific CD8 T cells around dendritic cells
    Hélène Beuneu
    G5 Dynamiques des Reponses Immunes, Equipe Avenir Inserm U668, Institut Pasteur, Paris, France
    J Immunol 177:1406-10. 2006
  8. pmc Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice
    Beatrice Breart
    G5 Dynamiques des Reponses Immunes, Institut Pasteur, Paris, France
    J Clin Invest 118:1390-7. 2008
  9. doi Subcapsular sinus macrophages promote NK cell accumulation and activation in response to lymph-borne viral particles
    Zacarias Garcia
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Blood 120:4744-50. 2012
  10. ncbi Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation
    Susanna Celli
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, F 75015, France
    Immunity 27:625-34. 2007

Collaborators

  • Matthew L Albert
  • Yves Levy
  • Toni Aebischer
  • Gerard Eberl
  • James P Di Santo
  • Guillaume Romet-Lemonne
  • Ellen A Robey
  • Shinichiro Sawa
  • Ofer Mandelboim
  • Mark Day
  • Marie France Carlier
  • Lucie Peduto
  • Nico Van Rooijen
  • Nadine Cerf-Bensussan
  • Olivier Schwartz
  • Serge Mostowy
  • Susanna Celli
  • Fabrice Lemaitre
  • Zacarias Garcia
  • Beatrice Breart
  • Hélène D Moreau
  • Jacques Deguine
  • Hélène Beuneu
  • Pervinder Sagoo
  • David Michonneau
  • Kym R Garrod
  • Andreas J Müller
  • Isabelle Bouvier
  • Orchidée Filipe-Santos
  • Ana Maria Lennon-Dumenil
  • Laure Guenin-Macé
  • Wilmara Salgado-Pabon
  • Caroline Demangel
  • Claire Biot
  • Matthias Lochner
  • CAROLYN E CLARK
  • Gerald F Späth
  • Georges A Azar
  • Fabrice Chretien
  • Gernot Sellge
  • Chiara Zurzolo
  • Ellen T Arena
  • Hui Hong
  • Armelle Phalipon
  • Philippe J Sansonetti
  • Elisabetta Frigimelica
  • Romain Veyron-Churlet
  • Marie Therese Ruf
  • Anne Danckaert
  • Maria Isabel Thoulouze
  • Peter F Leadlay
  • Laura Vedele
  • Katharina Nothelfer
  • Pascal Roux
  • Emmanuel Terriac
  • George N Thalmann
  • Hélène Jusforgues-Saklani
  • Alexander Bachmann
  • Georges Azar
  • Frédéric D Birkhäuser
  • Joel R Gsponer
  • Matthieu Piel
  • Carmen Molina-París
  • Cyrill A Rentsch
  • Grant Lythe
  • Charlotte Auriau
  • Milena Hasan
  • Johannes Snel
  • Valerie Gaboriau-Routhiau
  • Siona Hauer
  • Tahia Diana Fernandez
  • Marion Berard
  • Christoph Lippuner
  • Nicolas Glaichenhaus
  • Pascale Pescher
  • Aurélie Simon
  • Emmanuelle Pradelli

Detail Information

Publications39

  1. doi Functional immunoimaging: the revolution continues
    Philippe Bousso
    Institut Pasteur, Dynamics of Immune Responses Unit, F 75015 Paris, France
    Nat Rev Immunol 12:858-64. 2012
    ..These exciting developments further broaden the applications of immunoimaging and provide unprecedented opportunities to probe and decode immune cell communication in situ...
  2. ncbi Chronic infections capture little attention of the masses
    Philippe Bousso
    Dynamics of Immune Responses Unit, Department of Immunology, Institut Pasteur, Paris, France
    Immunity 34:699-701. 2011
    ....
  3. doi Intravital imaging reveals distinct dynamics for natural killer and CD8(+) T cells during tumor regression
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 33:632-44. 2010
    ..Altogether, our results offer mechanistic insight into how NK cells and CTLs can exert cytotoxic activity with remarkably different contact dynamics...
  4. doi Decoding the dynamics of T cell-dendritic cell interactions in vivo
    Susanna Celli
    G5 Dynamiques des Reponses Immunes, Departement d Immunologie, Institut Pasteur, Paris, France
    Immunol Rev 221:182-7. 2008
    ....
  5. pmc Competition for antigen determines the stability of T cell-dendritic cell interactions during clonal expansion
    Zacarias Garcia
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, F 75015 Paris, France
    Proc Natl Acad Sci U S A 104:4553-8. 2007
    ....
  6. doi Dynamic behavior of NK cells during activation in lymph nodes
    Hélène Beuneu
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, France
    Blood 114:3227-34. 2009
    ..That NK cells become activated while remaining motile may constitute an efficient strategy for sampling local concentrations of cytokines around DCs in secondary lymphoid tissues...
  7. ncbi Cutting edge: cognate CD4 help promotes recruitment of antigen-specific CD8 T cells around dendritic cells
    Hélène Beuneu
    G5 Dynamiques des Reponses Immunes, Equipe Avenir Inserm U668, Institut Pasteur, Paris, France
    J Immunol 177:1406-10. 2006
    ..Our results support a model in which CD4 help operates rapidly, in part by favoring CD8 T cells recruitment around those DCs that are the most competent for priming...
  8. pmc Two-photon imaging of intratumoral CD8+ T cell cytotoxic activity during adoptive T cell therapy in mice
    Beatrice Breart
    G5 Dynamiques des Reponses Immunes, Institut Pasteur, Paris, France
    J Clin Invest 118:1390-7. 2008
    ..The ability to visualize when, where, and how tumor cells are killed in vivo offers new perspectives for understanding how immune effectors survey cancer cells and how local tumor microenvironments may subvert immune responses...
  9. doi Subcapsular sinus macrophages promote NK cell accumulation and activation in response to lymph-borne viral particles
    Zacarias Garcia
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Blood 120:4744-50. 2012
    ..Our results identify SCS macrophages as primary mediators of NK cell activation in response to lymph-borne viral particles suggesting that they act as early sensors of local infection or delivery of viral-based vaccines...
  10. ncbi Real-time manipulation of T cell-dendritic cell interactions in vivo reveals the importance of prolonged contacts for CD4+ T cell activation
    Susanna Celli
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Paris, F 75015, France
    Immunity 27:625-34. 2007
    ..These results establish the importance of prolonged T cell-DC interactions for efficient CD4(+) T cell activation in vivo...
  11. doi Intravital two-photon imaging of natural killer cells and dendritic cells in lymph nodes
    Susanna Celli
    Departement d Immunologie, G5 Dynamiques des Reponses Immunes, Equipe Avenir, Institut Pasteur, Paris Cedex, France
    Methods Mol Biol 415:119-26. 2008
    ..Intravital two-photon imaging is a powerful tool to study the migration and cell interactions of immune cells such as NK and DC in physiological settings...
  12. doi A dynamic map of antigen recognition by CD4 T cells at the site of Leishmania major infection
    Orchidée Filipe-Santos
    Institut Pasteur, G5 Dynamiques des Reponses Immunes, Inserm U668, Equipe Avenir, Paris F 75724, France
    Cell Host Microbe 6:23-33. 2009
    ..Thus, disparities in the dynamics of T cell contacts with infected cells and local variation in T cell access to infected cells are important elements of the host-pathogen interplay...
  13. doi The PD-1 Axis Enforces an Anatomical Segregation of CTL Activity that Creates Tumor Niches after Allogeneic Hematopoietic Stem Cell Transplantation
    David Michonneau
    Dynamics of Immune Responses Unit, Equipe Labellisée Ligue contre le Cancer, Institut Pasteur, 75015 Paris, France Inserm U668, rue du Dr Roux, 75015 Paris, France Cellule Pasteur, University Paris Diderot, Sorbonne Paris Cité, 75015 Paris, France
    Immunity 44:143-54. 2016
    ..Our results uncover a mechanism generating an anatomical segregation of CTL activity that might dictate sites of GVHD and create niches for tumor escape. ..
  14. doi In vivo imaging of inflammasome activation reveals a subcapsular macrophage burst response that mobilizes innate and adaptive immunity
    Pervinder Sagoo
    Dynamics of Immune Responses Unit, Institut Pasteur, Paris, France
    Nat Med 22:64-71. 2016
    ....
  15. pmc Signal strength regulates antigen-mediated T-cell deceleration by distinct mechanisms to promote local exploration or arrest
    Hélène D Moreau
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France Inserm U668, 75015 Paris, France Cellule Pasteur, Sorbonne Paris Cité, University Paris Diderot, 75015 Paris, France
    Proc Natl Acad Sci U S A 112:12151-6. 2015
    ....
  16. doi Cutting Edge: A dual role for type I IFNs during polyinosinic-polycytidylic acid-induced NK cell activation
    Hélène Beuneu
    Institut Pasteur, Dynamics of Immune Responses Unit, F 75015 Paris, France
    J Immunol 187:2084-8. 2011
    ..Our results suggest that type I IFNs produced following viral infection simultaneously target accessory cells for IL-15 transpresentation and NK cells themselves and that these two pathways cooperate for NK cell priming...
  17. doi Dynamic in situ cytometry uncovers T cell receptor signaling during immunological synapses and kinapses in vivo
    Hélène D Moreau
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France
    Immunity 37:351-63. 2012
    ..As illustrated here, the DISC approach should provide unique opportunities to link immune cell behavior to phenotype and function in vivo...
  18. doi Phenotypic CD8+ T cell diversification occurs before, during, and after the first T cell division
    Fabrice Lemaitre
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France
    J Immunol 191:1578-85. 2013
    ..Our results support the idea that T cell diversification is a continuous process, initiated before clonal expansion and amplified during the first and subsequent cell divisions. ..
  19. pmc Subcellular dynamics of T cell immunological synapses and kinapses in lymph nodes
    Georges A Azar
    G5 Dynamiques des Reponses Immunes, Institut Pasteur, 75015 Paris, France
    Proc Natl Acad Sci U S A 107:3675-80. 2010
    ..Furthermore, we provide a dynamic look at immunological kinapses and synapses in lymph nodes and suggest the existence of distinct patterns of LAT redistribution during antigen recognition...
  20. ncbi Intravital two-photon imaging of T-cell priming and tolerance in the lymph node
    Susanna Celli
    Department d Immunologie, Institut Pasteur, Paris, France
    Methods Mol Biol 380:355-63. 2007
    ..This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts...
  21. doi T-cell activation by dendritic cells in the lymph node: lessons from the movies
    Philippe Bousso
    G5 Dynamiques des Réponses Immunea, Equipe Avenir, Inserm U668, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris 15, France
    Nat Rev Immunol 8:675-84. 2008
    ..This Review focuses on how this emerging field is changing our perception of T-cell activation by DCs...
  22. doi Dissecting T cell contraction in vivo using a genetically encoded reporter of apoptosis
    Kym R Garrod
    Institut Pasteur, Dynamics of Immune Responses Unit, 75015 Paris, France Inserm U668, 75015 Paris, France
    Cell Rep 2:1438-47. 2012
    ..Our results offer insight into a poorly explored phase of immunity and provide a versatile methodology to study apoptosis during the development or function of a variety of immune cells in vivo...
  23. doi How many dendritic cells are required to initiate a T-cell response?
    Susanna Celli
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Blood 120:3945-8. 2012
    ..In mice and probably humans, we estimate that a minimum of 85 DCs are required to initiate a T-cell response when starting from precursor frequency of 10(-6). Our results have implications for the rational design of DC-based vaccines...
  24. ncbi Real-time imaging of T-cell development
    Philippe Bousso
    Institut National de la Sante et de la Recherche Medicale U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Curr Opin Immunol 16:400-5. 2004
    ..Resolving thymocyte behavior at the single-cell level should help to clarify the mode of trafficking in the thymus and identify the spatio-temporal aspects of thymic selection events...
  25. ncbi Dynamic behavior of T cells and thymocytes in lymphoid organs as revealed by two-photon microscopy
    Philippe Bousso
    Unité U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Immunity 21:349-55. 2004
    ..In this review we summarize what we have learned so far and point out areas for future investigation using this approach...
  26. ncbi Dynamics of CD8+ T cell priming by dendritic cells in intact lymph nodes
    Philippe Bousso
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Immunol 4:579-85. 2003
    ..Taken together, our results identify the cellular behaviors that promote an efficient CD8+ T cell response in the lymph node...
  27. doi Visualizing how T cells collect activation signals in vivo
    Hélène D Moreau
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France Inserm U668, 75015 Paris, France Univ Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, rue du Dr Roux, F 75015 Paris, France Electronic address
    Curr Opin Immunol 26:56-62. 2014
    ..These recent progresses are providing new insights into how T cells sense antigen, collect activation signals during distinct types of interaction and integrate information over successive encounters. ..
  28. doi Cutting edge: tumor-targeting antibodies enhance NKG2D-mediated NK cell cytotoxicity by stabilizing NK cell-tumor cell interactions
    Jacques Deguine
    Dynamics of Immune Responses Unit, Institut Pasteur, 75015 Paris, France
    J Immunol 189:5493-7. 2012
    ..Thus, complementary signals mediated by recognition of stress ligands and tumor-specific Abs may contribute to the efficacy of NK cells during mAb therapy...
  29. ncbi Cellular orchestration of T cell priming in lymph nodes
    Beatrice Breart
    G5 Dynamiques des Reponses Immunes, Equipe Avenir, Inserm U668, Institut Pasteur, Paris, France
    Curr Opin Immunol 18:483-90. 2006
    ..Recent studies have begun to depict the cellular orchestration of T cell priming and to analyze the way it could influence the outcome of T cell responses...
  30. doi Dynamics of NK cell interactions in vivo
    Jacques Deguine
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    Immunol Rev 251:154-9. 2013
    ..We review here our recent understanding of NK cell interactions in vivo at steady state, during inflammation, and during tumor elimination, emphasizing the similarities and differences with T cells...
  31. pmc CD4 T cells integrate signals delivered during successive DC encounters in vivo
    Susanna Celli
    G5 Dynamiques des Reponses Immunes, INSERM 668, Departement d Immunologie, Institut Pasteur, Paris, France
    J Exp Med 202:1271-8. 2005
    ..Thus, repeated encounters with Ag-bearing DCs can occur in vivo and modulate CD4 T cell differentiation programs...
  32. doi Preexisting BCG-specific T cells improve intravesical immunotherapy for bladder cancer
    Claire Biot
    Unité d Immunobiologie des Cellules Dendritiques, Department of Immunology, Institut Pasteur, F 75724 Paris, France
    Sci Transl Med 4:137ra72. 2012
    ....
  33. doi Visualizing the functional diversification of CD8+ T cell responses in lymph nodes
    Hélène Beuneu
    Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Departement d Immunologie, Paris, France
    Immunity 33:412-23. 2010
    ..Thus, the early set of T cell-DC interactions in lymph nodes largely contribute to the heterogeneity of T cell responses through the generation of functionally divergent clonal progenies...
  34. doi Restricted microbiota and absence of cognate TCR antigen leads to an unbalanced generation of Th17 cells
    Matthias Lochner
    Lymphoid Tissue Development Unit, Department of Immunology, Institut Pasteur, 75724 Paris, France
    J Immunol 186:1531-7. 2011
    ..These results demonstrate that a complex microbiota and cognate Ag are required to generate a properly regulated set of RORγt(+) T cells and Th17 cells...
  35. doi CD4+ T cells rely on a cytokine gradient to control intracellular pathogens beyond sites of antigen presentation
    Andreas J Müller
    Dynamics of Immune Responses, Institut Pasteur, Paris, France
    Immunity 37:147-57. 2012
    ..Our results thus demonstrate that CD4(+) T cells can exert their protective activity by engaging a minority of infected cells...
  36. doi Visualizing the innate and adaptive immune responses underlying allograft rejection by two-photon microscopy
    Susanna Celli
    Institut Pasteur, Unité des Dynamiques des Réponses Immunes, Departement d Immunologie, Paris, France
    Nat Med 17:744-9. 2011
    ....
  37. pmc A role for the immediate early gene product c-fos in imprinting T cells with short-term memory for signal summation
    CAROLYN E CLARK
    Institut Pasteur, Dynamics of Immune Responses Unit, Paris, France
    PLoS ONE 6:e18916. 2011
    ....
  38. pmc Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation
    Laure Guenin-Macé
    Institut Pasteur, Unité d Immunobiologie de l Infection, Paris, France
    J Clin Invest 123:1501-12. 2013
    ..Our findings should allow for the rationale design of competitive inhibitors of mycolactone binding to N-WASP, with anti-Buruli ulcer therapeutic potential...
  39. pmc Shigella impairs T lymphocyte dynamics in vivo
    Wilmara Salgado-Pabon
    Molecular Microbial Pathogenesis Unit, Department Cell Biology and Infection, Institut National de Santé et de Recherche Médicale U786, 75724 Paris Cedex 15, France
    Proc Natl Acad Sci U S A 110:4458-63. 2013
    ..These findings indicate that S. flexneri targets T lymphocytes in vivo and highlight the role of type III effector secretion in modulating host adaptive immune responses...