Genomes and Genes
Affiliation: Institut Pasteur
- Normal and pathological expression of GFAP promoter elements in transgenic miceM Galou
Unité de Biochimie des Antigènes, Institut Pasteur, Paris, France
Glia 12:281-93. 1994..These transgenic lines provide a useful tool by enabling further studies of astroglial and, probably, neuronal physiologies...
- Hepatocyte nuclear factor 1 inactivation results in hepatic dysfunction, phenylketonuria, and renal Fanconi syndromeM Pontoglio
Unite des Virus Oncogenes, Departement des Biotechnologies, InstitutPasteur, Paris, France
Cell 84:575-85. 1996..The resulting massive urinary glucose loss leads to energy and water wasting. HNF1-deficient mice may provide a model for human renal Fanconi syndrome...
- Impaired wound healing in embryonic and adult mice lacking vimentinB Eckes
Department of Dermatology, University of Cologne, Koln, Germany
J Cell Sci 113:2455-62. 2000..These observations reveal an in vivo phenotype for the vimentin-deficient mouse, and challenge the dogma that key morphogenetic events occurring during development require generation of significant tractional forces by mesenchymal cells...
- Genome engineering via homologous recombination in mouse embryonic stem (ES) cells: an amazingly versatile tool for the study of mammalian biologyC Babinet
Unite de Biologie du Developpement, Institut Pasteur, CNRS URA 1960, Paris, France
An Acad Bras Cienc 73:365-83. 2001..In this paper, we present an overview of the methods and scenarios used for the programmed modification of mouse genome, and we underline their enormous interest for the study of mammalian biology...
- Teratocarcinomas induced by embryonic stem (ES) cells lacking vimentin: an approach to study the role of vimentin in tumorigenesisF Langa
Unite de Biologie du Developpement, URA C N R S Institut Pasteur, Paris, France
J Cell Sci 113:3463-72. 2000..Taken together, these results demonstrate that in this model vimentin is not essential for efficient tumour growth and differentiation in vivo...
- Transgenic mice: an irreplaceable tool for the study of mammalian development and biologyC Babinet
Developmental Biology Unit, Pasteur Institute, URA CNRS 1960, Paris, France
J Am Soc Nephrol 11:S88-94. 2000..Thus, the transgenic methodology affords a unique and irreplaceable tool for the study of mammalian development and biology and for the creation of animal models for human genetic diseases...
- The murine SNF5/INI1 chromatin remodeling factor is essential for embryonic development and tumor suppressionA Klochendler-Yeivin
Unite des Virus Oncogenes, CNRS URA 1644, Institut Pasteur, Paris, France
EMBO Rep 1:500-6. 2000..Furthermore, heterozygous mice develop nervous system and soft tissue sarcomas. In these tumors the wild-type allele was lost, providing further evidence that SNF5 functions as a tumor suppressor gene in certain cell types...
- Disrupted glial fibrillary acidic protein network in astrocytes from vimentin knockout miceM Galou
Unité de Biochimie des Antigènes, Institut Pasteur, Paris, France
J Cell Biol 133:853-63. 1996..In these cells, therefore, vimentin appears necessary to stabilize GFAP filaments and consequently the network formation...
- A 2-Mb YAC/BAC-based physical map of the ovum mutant (Om) locus region on mouse chromosome 11M Cohen-Tannoudji
Unite de Biologie du Developpement, CNRS URA 1960, Institut Pasteur, 25 rue du Dr Roux, Paris Cedex 15, 75724, France
Genomics 68:273-82. 2000..Two other genes (Rad51l3 and Schlafen 2) and one EST (D11Wsu78e) were also mapped in the Om region. This integrated map provides support for the identification of additional candidate genes for the DDK syndrome...
- Desmin is essential for the tensile strength and integrity of myofibrils but not for myogenic commitment, differentiation, and fusion of skeletal muscleZ Li
Station Centrale de Microscopie Electronique, Institut Pasteur, Paris, France
J Cell Biol 139:129-44. 1997..The results presented here show that desmin is essential to maintain the structural integrity of highly solicited skeletal muscle...
- Mutation in the Trapalpha/Ssr1 gene, encoding translocon-associated protein alpha, results in outflow tract morphogenetic defectsK Mesbah
Laboratoire EGDM, CNRS URA 2578, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
Mol Cell Biol 26:7760-71. 2006....
- BALB/c alleles at modifier loci increase the severity of the maternal effect of the "DDK syndrome"S Le Bras
Unite de Biologie du Developpement, URA CNRS 1960, Departement d Immunologie, Institut Pasteur, 75724 Paris Cedex 15, France
Genetics 154:803-11. 2000..These results indicate that BALB/c alleles at modifier loci increase the severity of the DDK syndrome...
- Healthy mice with an altered c-myc gene: role of the 3' untranslated region revisitedF Langa
Unite de Biologie du Developpement, CNRS URA 1960, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France
Oncogene 20:4344-53. 2001..Moreover, mice homozygous for the 3' UTR-deleted gene were perfectly healthy and fertile. Our results therefore strongly suggest that the 3' UTR of c-myc mRNA does not play a major role in the developmental control of c-myc expression...
- Null mutation of the prolactin receptor gene produces multiple reproductive defects in the mouseC J Ormandy
Institut National de la Santé et de la Recherche Médicale INSERM Unité 344, Endocrinologie Moléculaire Faculté de Médecine Necker, Paris, France
Genes Dev 11:167-78. 1997..This work establishes the prolactin receptor as a key regulator of mammalian reproduction, and provides the first total ablation model to further study the role of the prolactin receptor and its ligands...
- Impaired flow-induced dilation in mesenteric resistance arteries from mice lacking vimentinD Henrion
Institut National de la Santé et de la Recherche Médicale INSERM U 141, IFR 6 Circulation Lariboisière, Hopital Lariboisiere, Paris, France
J Clin Invest 100:2909-14. 1997..Thus, the absence of vimentin decreased selectively the response of resistance arteries to flow, suggesting a role for vimentin in the mechanotransduction of shear stress...
- Essential role for the homeoprotein vHNF1/HNF1beta in visceral endoderm differentiationC Coffinier
Unite des Virus Oncogenes, CNRS URA 1644, and Unité de Biologie du Développement, CNRS URA 1960, Institut Pasteur, F 75724 Paris Cedex 15, France
Development 126:4785-94. 1999..The examination of chimeric embryos suggests that vHNF1 expression might be cell-autonomously required in the gut for the proper morphogenesis of the embryo...
- Gene targeting the myf-5 locus with nlacZ reveals expression of this myogenic factor in mature skeletal muscle fibres as well as early embryonic muscleS Tajbakhsh
Department of Molecular Biology, CNRS URA1947, Pasteur Institute, Paris, France
Dev Dyn 206:291-300. 1996..These results suggest that the myf-5 gene is not activated in only a subset of muscle cells and are consistent with the results on the MyoD knockout mice...
- Gene trap insertion reveals two open reading frames in the mouse SSeCKS gene: the form predominantly detected in the nervous system is suppressed by the insertion while the other, specific of the testis, remains expressedA Camus
Unite de Biologie du Developpement, URA CNRS 1960, Institut Pasteur, 25 rue du Dr Roux, 75724 Cedex 15, Paris, France
Mech Dev 105:79-91. 2001..Nevertheless and surprisingly, these mice do not exhibit any obvious phenotype. The functional implications of these observations are discussed...
- Mice lacking vimentin develop and reproduce without an obvious phenotypeE Colucci-Guyon
Unité de Recherche Associée 361, Centre National de la Recherche Scientifique, Institut Pasteur, France
Cell 79:679-94. 1994....
- Krox-20 controls myelination in the peripheral nervous systemP Topilko
Unité 368 de l Institut National de la Santé et de la Recherche Médicale, Ecole Normale Superieure, Paris, France
Nature 371:796-9. 1994..Therefore Krox-20 is likely to control a set of genes required for completion of myelination in the peripheral nervous system...
- Altered control of cellular proliferation in the absence of mammalian brahma (SNF2alpha)J C Reyes
Unite de Biologie du Developpement, URA 1960 du CNRS, Institut Pasteur, Paris, France
EMBO J 17:6979-91. 1998..These studies suggest that BRM participates in the regulation of cell proliferation in adult mice...
- A high-resolution map around the locus Om on mouse Chromosome 11P A Baldacci
Unite de Biologie du Developpement, U R A C N R S 1960, Institut Pasteur, Paris, France
Mamm Genome 7:114-6. 1996..The distance between these two markers is about 2 cM. These data should facilitate the positional cloning and molecular characterization of Om...
- Transgenic mice carrying an Xist-containing YACE Heard
Unité de Génétique Moléculaire Murine, Institut Pasteur, Paris, France
Hum Mol Genet 5:441-50. 1996....
- Unexpected behavior of a gene trap vector comprising a fusion between the Sh ble and the lacZ genesA Camus
Departement d Immunologie, URA CNRS 1960, Institut Pasteur, Paris, France
Mol Reprod Dev 45:255-63. 1996..Germline chimeras were subsequently obtained and 15 mouse lines have been established. Intercrosses of animals heterozygous for the insertion revealed a mutant phenotype in several lines...
- Cerebellar defect and impaired motor coordination in mice lacking vimentinE Colucci-Guyon
Unite de Biologie du Developpement, Institut Pasteur, Paris, France
Glia 25:33-43. 1999..These observations demonstrate a role for vimentin in contributing to the normal development and morphology of BG and reveal a hitherto unreported functional relationship between BG and PC...
- Compartmentalization of lambda subtype expression in the B cell repertoire of mice with a disrupted or normal C kappa gene segmentP Sanchez
Departement d Immunologie, Institut Pasteur, UA CNRS 359, Paris, France
Int Immunol 6:711-9. 1994..Their prevalence is again observed regardless of the C kappa genotype and seems to be due to B1 cells. These results suggest that different mechanistic processes could control lambda subtype expression in B1 and B2 cell lineages...
- A transgenic model for listeriosis: role of internalin in crossing the intestinal barrierM Lecuit
, Station Centrale de Microscopie Electronique, Institut Pasteur, 75724 Paris Cedex 15, France
Science 292:1722-5. 2001..These results illustrate how relevant animal models for human infections can be generated...
- lacZ sequences prevent regulated expression of housekeeping genesM Cohen-Tannoudji
Unite de Biologie du Developpement, CNRS URA 1960, Institut Pasteur, 25 rue du Dr Roux, 75724, Paris, France
Mech Dev 90:29-39. 2000....
- Disruption of Krox-20 results in alteration of rhombomeres 3 and 5 in the developing hindbrainS Schneider-Maunoury
Unité 368 de l Institut National de la Santé et de la Recherche Médicale, Biologie Moléculaire du Développement, Ecole Normale Superieure, Paris, France
Cell 75:1199-214. 1993..We conclude that Krox-20, although not required for the initial delimitation of r3 and r5, plays an important role in the process of segmentation governing hindbrain development...
- Nonpermissiveness for mouse embryonic stem (ES) cell derivation circumvented by a single backcross to 129/Sv strain: establishment of ES cell lines bearing the Omd conditional lethal mutationC Kress
Unite de Biologie du Developpement, URA C N R S 1960, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cedex 15, France
Mamm Genome 9:998-1001. 1998..Such a scenario could be extended to the generation of ES cell lines bearing any mutation present in an otherwise nonpermissive mouse strain...
- Genomic structure and precise mapping of a thymic regulatory region on mouse chromosome 17 revealed by a c-myc transgene insertionA Lavenu
Department of Immunology, Institut Pasteur, 25 rue du Dr Roux, Paris Cedex, 75724, France
Genomics 34:381-8. 1996..Analysis of Tsil offers a unique opportunity to identify a regulatory region or a gene that might play an important role in T-cell maturation...
- Cardiovascular lesions and skeletal myopathy in mice lacking desminZ Li
Biologie Moleculaire de la Differenciation, SCME, Paris, France
Dev Biol 175:362-6. 1996..In conclusion, our results have demonstrated that desmin is not required for the differentiation of skeletal, cardiac, and smooth muscles but is essential to strengthen and maintain the integrity of these tissues...
- Reduction of renal mass is lethal in mice lacking vimentin. Role of endothelin-nitric oxide imbalanceF Terzi
INSERM U 380, Institut Cochin de Genetique Moleculaire, Paris, France
J Clin Invest 100:1520-8. 1997..These results suggest that vimentin plays a key role in the modulation of vascular tone, possibly via the tuning of endothelin-nitric oxide balance...
- The locus Om, responsible for the DDK syndrome, maps close to Sigje on mouse chromosome 11P A Baldacci
Unite de Genetique des Mammiferes, Institut Pasteur, Paris, France
Mamm Genome 2:100-5. 1992..Our results indicate that a single locus, Om, is responsible for the DDK syndrome and is located on Chromosome (Chr) 11, very close to the Sigje locus...
- MHC class I genes are not imprinted in the mouse placentaJ M Drezen
Department of Immunology, Institut Pasteur, Paris, France
Immunogenetics 40:62-5. 1994
- The DDK inbred strain as a model for the study of interactions between parental genomes and egg cytoplasm in mouse preimplantation developmentC Babinet
Institut Pasteur, Unite de Genetique des Mammiferes, Paris, France
Dev Suppl . 1990..These experiments should help in our understanding of nucleocytoplasmic interactions and the respective roles of parental genomes in early embryonic development...
- Linkage mapping of murine homolog of the yeast SPT6 gene to MMU11B1P W Chiang
Department of Pediatrics, University of Michigan Medical Center, Ann Arbor 48109 0650, USA
Mamm Genome 7:459-60. 1996
- Impaired mechanical stability, migration and contractile capacity in vimentin-deficient fibroblastsB Eckes
Department of Dermatology, University of Cologne, Federal Republic of Germany
J Cell Sci 111:1897-907. 1998....
- Introducing a null mutation in the mouse K6alpha and K6beta genes reveals their essential structural role in the oral mucosaP Wong
Department of Biological Chemistry and Department of Dermatology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
J Cell Biol 150:921-8. 2000..These studies demonstrate an essential structural role for K6 isoforms in the oral mucosa, and implicate filiform papillae as being the major stress bearing structures in dorsal tongue epithelium...
- A new family of mouse genes homologous to the human MAGE genesE De Plaen
Brussels Branch, 74 avenue Hippocrate UCL 74 59, Brussels, B1200, Belgium
Genomics 55:176-84. 1999..Expression of some Mage-a genes was also detected in tumor cell lines. Two Mage-a genes were found to be expressed in blastocysts...
- In vivo, villin is required for Ca(2+)-dependent F-actin disruption in intestinal brush bordersE Ferrary
Institut Curie, UMR 144, 75248 Paris, France
J Cell Biol 146:819-30. 1999..We postulate that this property might be involved in cellular plasticity related to cell injury...
- Targeted disruption of the murine junD gene results in multiple defects in male reproductive functionD Thepot
Laboratoire de Biologie Cellulaire et Moleculaire, INRA, Jouy en Josas, France
Development 127:143-53. 2000..No defects in fertility were observed in JunD-/- female animals. These results provide evidence for redundant functions for members of the Jun family during development and specific functions for JunD in male reproductive function...