Andrew D Griffiths

Summary

Country: France

Publications

  1. ncbi request reprint High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions
    Enrico Mastrobattista
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Chem Biol 12:1291-300. 2005
  2. ncbi request reprint Directed evolution by in vitro compartmentalization
    Oliver J Miller
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Nat Methods 3:561-70. 2006
  3. ncbi request reprint Kinetic aspects of emulsion stabilization by surfactants: a microfluidic analysis
    Jean Christophe Baret
    , , CNRS UMR 7006, Strasbourg, France
    Langmuir 25:6088-93. 2009
  4. ncbi request reprint Droplets as microreactors for high-throughput biology
    Valerie Taly
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Chembiochem 8:263-72. 2007
  5. ncbi request reprint Miniaturising the laboratory in emulsion droplets
    Andrew D Griffiths
    Institut de Science et d Ingénierie Supramoléculaires ISIS, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Trends Biotechnol 24:395-402. 2006
  6. doi request reprint A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
    Ali Fallah-Araghi
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, Strasbourg, France
    Lab Chip 12:882-91. 2012
  7. pmc High-resolution dose-response screening using droplet-based microfluidics
    Oliver J Miller
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7006, 8 allee Gaspard Monge, F 67083 Strasbourg cedex, France
    Proc Natl Acad Sci U S A 109:378-83. 2012
  8. doi request reprint Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity
    Jean Christophe Baret
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083, Strasbourg Cedex, France
    Lab Chip 9:1850-8. 2009
  9. doi request reprint Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme
    Linas Mazutis
    Institut de Science et d Ingénierie Supramoléculaire ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083, Strasbourg Cedex, France
    Lab Chip 9:2902-8. 2009
  10. doi request reprint Microfluidic platform for combinatorial synthesis in picolitre droplets
    Ashleigh B Theberge
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Lab Chip 12:1320-6. 2012

Collaborators

  • Jean Christophe Baret
  • Christoph A Merten
  • Linas Mazutis
  • Irina V Balyasnikova
  • Dan S Tawfik
  • Mingtan Hai
  • Jenifer Clausell-Tormos
  • Oliver J Miller
  • Bernard T Kelly
  • Valerie Taly
  • Abdeslam El Harrak
  • Lucas Frenz
  • Bachir El Debs
  • Enrico Mastrobattista
  • Shlomo Magdassi
  • Jeremy J Agresti
  • Kalia Bernath
  • Michael Ryckelynck
  • Estelle Mayot
  • Ashleigh B Theberge
  • Majdi Najah
  • Ali Fallah-Araghi
  • Darren R Link
  • Deniz Pekin
  • Lucia Granieri
  • Richard Williams
  • Amir Aharoni
  • Hans Leemhuis
  • Helen M Cohen
  • Yin Fai Lee
  • Armin Sepp
  • Thomas Mangeat
  • Eamonn K Rooney
  • Michael L Samuels
  • Ramesh Utharala
  • Pierre Dieu
  • Wilhelm T S Huck
  • Felix Kleinschmidt
  • Martin Galvan
  • Pierre Laurent-Puig
  • Florian Millot
  • Yousr Skhiri
  • Delphine Le Corre
  • Chaouki Ben Salem
  • Jonathan W Larson
  • J Brian Hutchison
  • Sylvie Begin-Colin
  • Katherine J Humphry
  • Sarah Köster
  • David A Weitz
  • Matthias Pauly
  • Joshua Blouwolff
  • Honey Duan
  • Diana Lieber
  • Christian Holtze
  • Abdeslam El-Harrak
  • Sergio G Peisajovich
  • Gil Amitai
  • Andres Jäschke
  • Florian Hollfelder
  • Viktor Stein
  • Estelle Chanudet
  • Patrick Treacy

Detail Information

Publications31

  1. ncbi request reprint High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions
    Enrico Mastrobattista
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
    Chem Biol 12:1291-300. 2005
    ..Only two specific mutations were ever seen to provide this improvement in Ebg beta-galactosidase activity in vivo. In contrast, nearly all the improved beta-galactosidases selected in vitro resulted from different mutations...
  2. ncbi request reprint Directed evolution by in vitro compartmentalization
    Oliver J Miller
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Nat Methods 3:561-70. 2006
  3. ncbi request reprint Kinetic aspects of emulsion stabilization by surfactants: a microfluidic analysis
    Jean Christophe Baret
    , , CNRS UMR 7006, Strasbourg, France
    Langmuir 25:6088-93. 2009
    ..Microfluidic emulsification devices can therefore be tailored to improve emulsification while decreasing the concentration of surfactant by increasing the time before the droplets first come into contact...
  4. ncbi request reprint Droplets as microreactors for high-throughput biology
    Valerie Taly
    Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK
    Chembiochem 8:263-72. 2007
    ..An exciting synergy is now emerging between these two fields, whereby the tools of ultrahigh-throughput screening promise to open up new directions in molecular engineering...
  5. ncbi request reprint Miniaturising the laboratory in emulsion droplets
    Andrew D Griffiths
    Institut de Science et d Ingénierie Supramoléculaires ISIS, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Trends Biotechnol 24:395-402. 2006
    ..This review describes the scope and potential of IVC in areas such as in vitro evolution of proteins and RNAs, cell-free cloning and sequencing, genetics, genomics, and proteomics...
  6. doi request reprint A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
    Ali Fallah-Araghi
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, Strasbourg, France
    Lab Chip 12:882-91. 2012
    ..Compared to screening using microtiter plate-based systems, the volume and cost of PCR and IVTT reagents are reduced by almost 10(5)-fold, allowing the screening of 10(6) genes using only 150 μL of reagents...
  7. pmc High-resolution dose-response screening using droplet-based microfluidics
    Oliver J Miller
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7006, 8 allee Gaspard Monge, F 67083 Strasbourg cedex, France
    Proc Natl Acad Sci U S A 109:378-83. 2012
    ..We identified a number of novel inhibitors, the most potent being sodium cefsulodine, which has an IC(50) of 27 ± 0.83 μM...
  8. doi request reprint Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity
    Jean Christophe Baret
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083, Strasbourg Cedex, France
    Lab Chip 9:1850-8. 2009
    ..In addition, single active droplets were sorted and cells were successfully recovered...
  9. doi request reprint Multi-step microfluidic droplet processing: kinetic analysis of an in vitro translated enzyme
    Linas Mazutis
    Institut de Science et d Ingénierie Supramoléculaire ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083, Strasbourg Cedex, France
    Lab Chip 9:2902-8. 2009
    ..Furthermore, the system also opens up the possibility of performing a wide range of other new (bio)chemical reactions in droplets...
  10. doi request reprint Microfluidic platform for combinatorial synthesis in picolitre droplets
    Ashleigh B Theberge
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Lab Chip 12:1320-6. 2012
    ....
  11. pmc Functional single-cell hybridoma screening using droplet-based microfluidics
    Bachir El Debs
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Proc Natl Acad Sci U S A 109:11570-5. 2012
    ..A wide variance in antibody expression levels at the single-cell level within a single hybridoma line was observed and high expressors could be successfully sorted and recultivated...
  12. doi request reprint High-throughput screening of enzymes by retroviral display using droplet-based microfluidics
    Lucia Granieri
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Chem Biol 17:229-35. 2010
    ..During a single selection cycle, a more than 1300-fold enrichment of the active wild-type enzyme was demonstrated...
  13. doi request reprint Quantitative and sensitive detection of rare mutations using droplet-based microfluidics
    Deniz Pekin
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Lab Chip 11:2156-66. 2011
    ....
  14. doi request reprint An automated two-phase microfluidic system for kinetic analyses and the screening of compound libraries
    Jenifer Clausell-Tormos
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8, allee Gaspard Monge, BP 70028, 67083, Strasbourg Cedex, France
    Lab Chip 10:1302-7. 2010
    ..g. for recording reaction kinetics). Hence the technology eliminates several bottlenecks of current droplet-based microfluidic systems and should open the way for (bio-)chemical and cell-based screens...
  15. doi request reprint Quantitative cell-based reporter gene assays using droplet-based microfluidics
    Jean Christophe Baret
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, BP 70028, F 67083 Strasbourg cedex, France
    Chem Biol 17:528-36. 2010
    ..Screening approximately 7500 cells per concentration provides statistically relevant data that allow precise measurement of the EC(50) (70 nM +/- 12%, alpha = 0.05), in agreement with standard methods as well as with literature data...
  16. ncbi request reprint Coupling the inhibition of viral transduction with a positive fluorescence signal
    Jenifer Clausell-Tormos
    Institut de Science et d Ingenierie Supramoleculaires, Universite de Strasbourg, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Comb Chem High Throughput Screen 13:352-7. 2010
    ..The assay allows Z-factors of approximately 0.9, takes cytotoxic side effects into account and could in theory be adapted for high-throughput screening of inhibitors against further viral species...
  17. ncbi request reprint Teaching single-cell digital analysis using droplet-based microfluidics
    Majdi Najah
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Anal Chem 84:1202-9. 2012
    ....
  18. doi request reprint A fast and efficient microfluidic system for highly selective one-to-one droplet fusion
    Linas Mazutis
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite de Strasbourg, 67083, Strasbourg Cedex, France
    Lab Chip 9:2665-72. 2009
    ....
  19. doi request reprint Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis
    Linas Mazutis
    Institut de Science et d Ingenierie Supramoleculaire, Universite de Strasbourg, CNRS UMR 7006, 8 allee Gaspard Monge, 67083 Strasbourg Cedex, France
    Anal Chem 81:4813-21. 2009
    ..The clonal amplified DNA in each 2 pL droplet was further analyzed by measuring the enzymatic activity of the encoded proteins after fusion with a 15 pL droplet containing an in vitro translation system...
  20. doi request reprint Droplet-based microreactors for the synthesis of magnetic iron oxide nanoparticles
    Lucas Frenz
    Institut de Science et d Ingénierie Supramoléculaires ISIS, Universite Louis Pasteur, CNRS UMR 7006, 8, allee Gaspard Monge, BP 70028, 67083 Strasbourg Cedex, France
    Angew Chem Int Ed Engl 47:6817-20. 2008
  21. ncbi request reprint In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
    Kalia Bernath
    Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
    Anal Biochem 325:151-7. 2004
    ..The ability of FACS instruments to sort up to 40000 events per second may endow this technology a wide potential in the area of high-throughput screening and the directed evolution of enzymes...
  22. ncbi request reprint Amplification of complex gene libraries by emulsion PCR
    Richard Williams
    MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Nat Methods 3:545-50. 2006
  23. ncbi request reprint Selective gene amplification
    Bernard T Kelly
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK
    Protein Eng Des Sel 20:577-81. 2007
    ..This approach mimics the process of natural selection; 'fitter' genes--encoding more efficient enzymes--have more 'offspring'. It allows selection for any activity so long as a product-specific ligand (e.g. an antibody) is available...
  24. pmc Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization
    Andrew D Griffiths
    MRC Laboratory of Molecular Biology, Cambridge CB2 2QH, UK
    EMBO J 22:24-35. 2003
    ..This completely in vitro process selects for all enzymatic features simultaneously (substrate recognition, product formation, rate acceleration and turnover) and single enzyme molecules can be detected...
  25. ncbi request reprint Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry
    Armin Sepp
    MRC Laboratory of Molecular Biology, MRC Centre, Hills Road, CB2 2QH, Cambridge, UK
    FEBS Lett 532:455-8. 2002
    ..Single beads can be isolated using flow sorting and the single gene on the bead amplified by polymerase chain reaction. Hence, the entire process can be performed completely in vitro...
  26. ncbi request reprint Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
    Helen M Cohen
    MRC Centre for Protein Engineering and MRC Laboratory for Molecular Biology, Cambridge CB2 2QH, UK
    Protein Eng Des Sel 17:3-11. 2004
    ..This study provides a rare example of a laboratory-evolved enzyme whose catalytic efficiency surpasses that of the wild-type enzyme with the principal substrate...
  27. ncbi request reprint High-throughput screens and selections of enzyme-encoding genes
    Amir Aharoni
    Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Opin Chem Biol 9:210-6. 2005
    ..We focus on HTS approaches that enable selection from large libraries (>10(6) gene variants) with relatively humble means (i.e. non-robotic systems), and on in vitro compartmentalization in particular...
  28. ncbi request reprint New genotype-phenotype linkages for directed evolution of functional proteins
    Hans Leemhuis
    Department of Biochemistry, Cambridge University, Cambridge CB2 1GA, UK
    Curr Opin Struct Biol 15:472-8. 2005
    ..New approaches such as mRNA display, ribosome display and in vitro compartmentalisation have complementary areas of application in selections for binding or catalysis...
  29. pmc Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization
    Jeremy J Agresti
    Laboratory of Molecular Biology, Medical Research Council, Hills Road, Cambridge CB2 2QH, United Kingdom
    Proc Natl Acad Sci U S A 102:16170-5. 2005
    ..More efficient Diels-Alderases may need to catalyze a second reaction that transforms the product and prevents product inhibition...
  30. doi request reprint Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms
    Jenifer Clausell-Tormos
    Institut de Science et d Ingenierie Supramoleculaires, Universite Louis Pasteur, Strasbourg Cedex, France
    Chem Biol 15:427-37. 2008
    ..This should open the way for high-throughput, cell-based screening that can use >1000-fold smaller assay volumes and has approximately 500x higher throughput than conventional microtiter plate assays...
  31. pmc Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation
    Yin Fai Lee
    The MRC Laboratory of Molecular Biology and Centre for Protein Engineering, MRC Centre, Hills Road, Cambridge CB2 2QH, UK
    Nucleic Acids Res 30:4937-44. 2002
    ..Our results suggest that recognition of base pairs 3 and 4 of the target site either relies entirely on main chain interactions or that different residues from those identified in the crystal structure contribute to DNA recognition...