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Genomes and Genes | Marc GrailleSummaryCountry: France Publications
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Publications
Methylation of class I translation termination factors: structural and functional aspectsMarc Graille
IBBMC, Universite Paris Sud 11, CNRS UMR8619, Orsay Cedex, F 91405, France
Biochimie 94:1533-43. 2012..However, methylation is catalysed by completely unrelated enzymes. The function of this motif and its post-translational modification will be discussed in the context of recent structural and functional studies...- Structure of yeast Dom34: a protein related to translation termination factor Erf1 and involved in No-Go decayMarc Graille
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, UMR8619 CNRS, IFR115, F 91405 Orsay, France
J Biol Chem 283:7145-54. 2008.... - Zinc induces structural reorganization of gelatin binding domain from human fibronectin and affects collagen bindingMarc Graille
IBBMC, Université Paris 11, IFR115, CNRS UMR8619, Orsay Cedex F 91405, France
Structure 18:710-8. 2010..These data suggest the Zn may play a regulatory role for the cellular functions of fibronectin... 
Mechanism of activation of methyltransferases involved in translation by the Trm112 'hub' proteinDominique Liger
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, CNRS UMR 8619, Orsay Cedex F 91405, France
Nucleic Acids Res 39:6249-59. 2011....- The structure of an archaeal homodimeric ligase which has RNA circularization activityMark Adrian Brooks
IBBMC CNRS, Universite de Paris Sud, CNRS UMR8619, IFR115, 91405 Orsay, France
Protein Sci 17:1336-45. 2008..Additionally, we show that the protein is indeed capable of circularizing RNA molecules in an ATP-dependent reaction. These structural and biochemical results provide an insight into the potential physiological roles of Pab1020... - Crystal structure of the YML079w protein from Saccharomyces cerevisiae reveals a new sequence family of the jelly-roll foldCong Zhao Zhou
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Centre National de la Recherche Scientifique Unité Mixte de Recherche 8619, Universite Paris Sud, 91405 Orsay, France
Protein Sci 14:209-15. 2005..The presence of a hydrophobic ligand in a well conserved pocket inside the barrel and local sequence similarity with bacterial epimerases may suggest a biochemical function for this protein... 
Molecular basis for bacterial class I release factor methylation by PrmCMarc Graille
Institut de Biochimie et Biophysique Moleculaire et Cellulaire, CNRS, UMR8619, Universite Paris Sud, Orsay, France
Mol Cell 20:917-27. 2005..Structural and mutagenic data indicate a compact conformation of RF1 that is unlike its conformation when it is bound to the ribosome but is similar to the crystal structure of the protein alone...- Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplexArnaud Hecker
Institut de Genetique et Microbiologie, Universite Paris Sud, IFR115 UMR8621 CNR, Orsay, France
EMBO J 27:2340-51. 2008..Direct interaction between Kae1p and Bud32p in yeast is required both for the transcription and the telomere homeostasis function of EKC/KEOPS... - Structure-based functional annotation: yeast ymr099c codes for a D-hexose-6-phosphate mutarotaseMarc Graille
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, Universite Paris Sud, IFR115, CNRS UMR8619, F 91405 Orsay cedex, France
J Biol Chem 281:30175-85. 2006..The metabolic role of a hexose-6-phosphate mutarotase is discussed. This work illustrates that structural information has been crucial to assign YMR099cp to the orphan EC activity: hexose-phosphate mutarotase... - Crystal structure of the yeast His6 enzyme suggests a reaction mechanismSophie Quevillon-Cheruel
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, 91405 Orsay, France
Protein Sci 15:1516-21. 2006..Asp9 is conserved in yeast His6 and bacterial or archaeal HisA sequences, and Asp134 has equivalents in both HisA and TrpF, but they occur at a different position in the protein sequence... - Crystal structure of yeast YHR049W/FSH1, a member of the serine hydrolase familySophie Quevillon-Cheruel
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
Protein Sci 14:1350-6. 2005..This finding was further strengthened by the unexpected presence of a compound covalently bound to the catalytic serine in the active site. Apparently, the enzyme was trapped with a reactive compound during the purification process... - A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse IIINicolas Leulliot
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Orsay, France
EMBO J 23:2468-77. 2004..The results suggest that this helix allows the Rnt1p dsRBD to bind to short RNA stem-loops by modulating the conformation of helix alpha1, a key RNA-recognition element of the dsRBD... - Crystal structure of yeast YER010Cp, a knotable member of the RraA protein familyNicolas Leulliot
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
Protein Sci 14:2751-8. 2005..A tunnel at the interface between two monomers, lined with conserved charged residues, has unassigned residual electron density and may constitute an active site for a yet unknown activity... - The universal Kae1 protein and the associated Bud32 kinase (PRPK), a mysterious protein couple probably essential for genome maintenance in Archaea and EukaryaArnaud Hecker
Universite Paris Sud, Institut de Genetique et Microbiologie, IFR115, UMR8621 CNRS, 91405 Orsay Cedex, France
Biochem Soc Trans 37:29-35. 2009..Understanding the precise biochemical function and biological role of these two proteins (which are probably essential for genome maintenance) remains a major challenge... - The yeast ribosome synthesis factor Emg1 is a novel member of the superfamily of alpha/beta knot fold methyltransferasesNicolas Leulliot
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619, Bat 430, Universite de Paris Sud, 91405 Orsay Cedex, France
Nucleic Acids Res 36:629-39. 2008..We conclude that the catalytic activity of Emg1 is not essential and that the presence of the protein is both necessary and sufficient for ribosome biogenesis... - Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanismsMarc Graille
Institut de Biochimie et de Biophysique Moleculaire et Cellulaire, UMR8619 CNRS, Universite Paris Sud, IFR115, F 91405 Orsay, France
Structure 16:360-70. 2008..This structure, combined with a wealth of experimental data gathered on these enzymes, allows us to propose a structural model for the two-gate DNA transfer mechanism... - Functional and structural characterization of PaeM, a colicin M-like bacteriocin produced by Pseudomonas aeruginosaHélène Barreteau
Universite Paris Sud, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, UMR 8619, F 91405 Orsay, France
J Biol Chem 287:37395-405. 2012..This isolated domain of the PaeM bacteriocin was further shown to kill E. coli cells when addressed to the periplasm of these bacteria... - The structure of the NasR transcription antiterminator reveals a one-component system with a NIT nitrate receptor coupled to an ANTAR RNA-binding effectorMarion Boudes
IBBMC CNRS UMR8619, Bat 430, Universite Paris Sud, 91405 Orsay, France
Mol Microbiol 85:431-44. 2012..The resulting structural movements in the NIT domain would provoke an active configuration of the ANTAR domains necessary for specific leader mRNA binding... - High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?Nicolas Leulliot
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS UMR 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
Acta Crystallogr D Biol Crystallogr 61:664-70. 2005..This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples... - Structures of in vitro evolved binding sites on neocarzinostatin scaffold reveal unanticipated evolutionary pathwaysAntoine Drevelle
, IBBMC, CNRS, UMR8619, , , 91405 Orsay, France
J Mol Biol 358:455-71. 2006..Comparison of the three complexed forms clearly suggests that these unanticipated structural responses resulted from the molecular arrangement used for the selection experiments... - Design, production and molecular structure of a new family of artificial alpha-helicoidal repeat proteins (αRep) based on thermostable HEAT-like repeatsAgathe Urvoas
Institut de Biochimie Moléculaire et Cellulaire IBBMC, Univ Paris Sud, UMR 8619, Orsay, F 91405 Orsay, France
J Mol Biol 404:307-27. 2010..This fortuitous binding site could later prove to be a useful binding site for other low molecular mass partners... - Surveillance pathways rescuing eukaryotic ribosomes lost in translationMarc Graille
Institut de Biochimie et Biophysique Moléculaire et Cellulaire IBBMC, Centre National de la Recherche Scientifique CNRS, UMR8619, Bat 430, Universite Paris Sud, F 91405 Orsay cedex, France, and at the Laboratoire de Biochimie, CNRS, UMR 7654, Ecole Polytechnique, F 91128 Palaiseau cedex, France
Nat Rev Mol Cell Biol 13:727-35. 2012..Recent studies support a general molecular framework for these surveillance pathways, the mechanisms of which are intimately related to translation termination... - X-ray structure and site-directed mutagenesis analysis of the Escherichia coli colicin M immunity proteinFabien Gérard
Laboratoire des Enveloppes Bactériennes et Antibiotiques, Institut de Biochimie et Biophysique Moleculaire et Cellulaire, UMR 8619, Universite Paris Sud, Orsay F 91405, France
J Bacteriol 193:205-14. 2011..The involvement of DsbA oxidase in the formation of the Cmi disulfide bond is also demonstrated... - The HSP90 and DNA topoisomerase VI inhibitor radicicol also inhibits human type II DNA topoisomeraseDanièle Gadelle
Institut de Genetique et Microbiologie, UMR CNRS 8621, France
Biochem Pharmacol 72:1207-16. 2006..The finding that radicicol can inhibit at least two different antitumoral drug targets in human, and interferes with drugs currently used in cancer treatment, could have implications in cancer therapy... - An archaeal orthologue of the universal protein Kae1 is an iron metalloprotein which exhibits atypical DNA-binding properties and apurinic-endonuclease activity in vitroArnaud Hecker
Institut de Genetique et Microbiologie, Univ Paris Sud, IFR115, UMR8621 CNRS, 91405 Orsay, France
Nucleic Acids Res 35:6042-51. 2007..Kae1 is thus a novel and atypical universal DNA interacting protein whose importance could rival those of RecA (RadA/Rad51) in the maintenance of genome integrity in all living cells... - Crystal structure of yeast allantoicase reveals a repeated jelly roll motifNicolas Leulliot
Institut de Biochimie et de Biophysique Moléculaire et Cellulaire CNRS Unité Mixte de Recherche 8619, Universite Paris Sud, Batiment 430, 91405 Orsay, France
J Biol Chem 279:23447-52. 2004..The hexameric packing concentrates these patches into conserved pockets that probably constitute the active site... - Crystal structure of the bifunctional chorismate synthase from Saccharomyces cerevisiaeSophie Quevillon-Cheruel
, , , 91405 Orsay, France
J Biol Chem 279:619-25. 2004..This hypothesis is supported by previously published spectroscopic measurements implying that the enzyme undergoes considerable structural changes upon binding of both FMN and EPSP... - Structure of protein phosphatase methyltransferase 1 (PPM1), a leucine carboxyl methyltransferase involved in the regulation of protein phosphatase 2A activityNicolas Leulliot
, , , 91405 Orsay, France
J Biol Chem 279:8351-8. 2004..The structure of PPM1 in a second crystal form provides clues to the dynamic nature of the PPM1/PP2A interaction... - Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphateCong-Zhao Zhou
, , , 91405 Orsay, France
J Biol Chem 278:50371-6. 2003..The structure of the free and bound protein are globally similar but show some significant differences in a region containing a polyproline peptide and a putative membrane attachment site... - Crystal structure and functional characterization of yeast YLR011wp, an enzyme with NAD(P)H-FMN and ferric iron reductase activitiesDominique Liger
, , , 91405 Orsay, France
J Biol Chem 279:34890-7. 2004..OY1-2 azoreductase. We show that YLR011wp has an NAD(P)H-dependent FMN reductase and a strong ferricyanide reductase activity. We further demonstrate a weak but specific reductive activity on azo dyes and nitrocompounds... - Crystal structure of the YGR205w protein from Saccharomyces cerevisiae: close structural resemblance to E. coli pantothenate kinaseInes Li de La Sierra-Gallay
Laboratoire d Enzymologie et Biochimie Structurales CNRS UPR 9063, Bat 34, 1 Av de la Terrasse, 91198 Gif sur Yvette, France
Proteins 54:776-83. 2004..The nature of the phosphate accepting substrate remains to be determined... - Structural inhibition of the colicin D tRNase by the tRNA-mimicking immunity proteinMarc Graille
LEBS, CNRS, UPR 9063, Gif sur Yvette, France
EMBO J 23:1474-82. 2004.... - Catalytic mechanism and structure of viral flavin-dependent thymidylate synthase ThyXSébastien Graziani
CNRS, UMR 7645, Laboratory of Optics and Biosciences, Ecole Polytechnique, 91128 Palaiseau, France
J Biol Chem 281:24048-57. 2006..Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate... - Crystal structure of the YDR533c S. cerevisiae protein, a class II member of the Hsp31 familyMarc Graille
Laboratoire d'Enzymologie et Biochimie Structurales, CNRS-UPR 9063, , 1 Avenue de la Terrasse, 91198 Gif sur Yvette, France
Structure 12:839-47. 2004..Detailed structural analysis strongly suggests that the YDR533c protein crystal structure is the first one of a class II member of the Hsp31 family... - The Paris-Sud yeast structural genomics pilot-project: from structure to functionSophie Quevillon-Cheruel
, , , 91405 Orsay, France
Biochimie 86:617-23. 2004..Strategies and final statistics are evaluated. We finally discuss the opportunity of structural genomics programs to contribute to functional biochemical annotation... - Activation of the LicT transcriptional antiterminator involves a domain swing/lock mechanism provoking massive structural changesMarc Graille
Laboratoire d Enzymologie et Biochimie Structurales, CNRS UPR9063, Gif sur Yvette, France
J Biol Chem 280:14780-9. 2005..Our results suggest that PRD2 is highly mobile in the native protein, whereas it is locked upon activation by phosphorylation... - The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeastValérie Heurgué-Hamard
UPR 9073 du CNRS, Institut de Biologie Physico Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France
J Biol Chem 281:36140-8. 2006..The Ynr046w eRF1 methyltransferase subunit is shared by the tRNA methyltransferase Trm11p and probably by two other enzymes containing a Rossman fold... - Fine tuning of the specificity of an anti-progesterone antibody by first and second sphere residue engineeringOlivier Dubreuil
, , , , Centre d'Etudes de Saclay, Gif-sur-Yvette Cedex 91191, France
J Biol Chem 280:24880-7. 2005..The improvements are partly linked to a change in the canonical class of the light chain third hypervariable loop... - Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune responseMarc Graille
, Centre d'Etudes de Saclay, 91191 Gif-sur-Yvette, France
J Mol Biol 354:447-58. 2005..This study provides the first structural description of the monomeric form of SAG1, and significant insights into its dual role of adhesin and immune target during parasite infection... - Evidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interfaceMarc Graille
, , , Centre d'Etudes de Saclay, Gif-sur-Yvette, France
J Biol Chem 277:47500-6. 2002..These sites exhibit a remarkable structural mimicry of growth factors binding to their receptors. This could explain the protein L superantigenic activity on human B lymphocytes... - Observation and characterization of the interaction between a single immunoglobulin binding domain of protein L and two equivalents of human kappa light chainsNicholas G Housden
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, Hants SO16 7PX, United Kingdom
J Biol Chem 279:9370-8. 2004..4 +/- 0.8 microM in good agreement with the value of 4.6 +/- 0.8 microM determined by isothermal titration calorimetry. The mutation Y53F reduces the affinity of site 1 to approximately that of site 2... - HalX: an open-source LIMS (Laboratory Information Management System) for small- to large-scale laboratoriesJaime Prilusky
Israel Structural Proteomics Center, Weizmann Institute of Science, Rehovot 76100, Israel
Acta Crystallogr D Biol Crystallogr 61:671-8. 2005..This should lead to much improved management and tracking of structural genomics experimental data... - Protein L mutants for the crystallization of antibody fragmentsEnrico A Stura
CEA, Département d Ingénierie et d Etudes des Protéines DIEP, CE Saclay, 91191 Gif sur Yvette Cedex, France
Acta Crystallogr D Biol Crystallogr 58:1744-8. 2002..In this study, we have selected PpL mutants with different behavior and that are most useful for crystallization and we present the various packing modes obtained so far...