To T, Grandvalet C, Alexandre H, Tourdot MarÃ©chal R. Cyclopropane fatty acid synthase from Oenococcus oeni: expression in Lactococcus lactis subsp. cremoris and biochemical characterization. Arch Microbiol. 2015;197:1063-74 pubmed publisher
..oeni enzyme. The current study reveals an essential hypothesis about the specificity of O. oeni CFA synthase which could play a key function in the acid tolerance mechanisms of this enological bacterium. ..
Darsonval M, Msadek T, Alexandre H, Grandvalet C. The Antisense RNA Approach: a New Application for In Vivo Investigation of the Stress Response of Oenococcus oeni, a Wine-Associated Lactic Acid Bacterium. Appl Environ Microbiol. 2016;82:18-26 pubmed publisher
..This study describes a new, efficient tool to demonstrate the use of antisense technology for modulating gene expression in O. oeni. ..
Darsonval M, Alexandre H, Grandvalet C. Genetically engineered Oenococcus oeni strains to highlight the impact of estA2 and estA7 esterase genes on wine ester profile. Food Microbiol. 2016;60:21-8 pubmed publisher
..EstA7 has synthetic activity toward hexyl acetate with a significant 22.7% increase. This study reports the first efficient expression system enabling the production of a functional protein in O. oeni in enological conditions. ..
Grandvalet C, Coucheney F, Beltramo C, Guzzo J. CtsR is the master regulator of stress response gene expression in Oenococcus oeni. J Bacteriol. 2005;187:5614-23 pubmed
..Unlike the multiple regulatory mechanisms of stress response described in many closely related gram-positive bacteria, this is the first example where dnaK and groESL are controlled by CtsR but not by HrcA. ..
Grandvalet C, Assad Garc a J, Chu Ky S, Tollot M, Guzzo J, Gresti J, et al
. Changes in membrane lipid composition in ethanol- and acid-adapted Oenococcus oeni cells: characterization of the cfa gene by heterologous complementation. Microbiology. 2008;154:2611-9 pubmed publisher
..oeni. In spite of this partial complementation, the complemented strain of E. coli totally recovered its viability after ethanol shock (10 %, v/v) whereas its viability was only partly recovered after an acid shock at pH 3.0...